Various Uses of Lycopene: Comparison
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Lycopene is a carotenoid abundantly found in red vegetables. This natural pigment displays an important role in human biological systems due to its excellent antioxidant and health-supporting functions, which show a protective effect against cardiovascular diseases, hypertension, cancers, and diabetes.

  • lycopene
  • carotenoids
  • isomers
  • antioxidants
  • cancers
  • food technology
  • pharmaceuticals
  • cosmetics
  • biotechnology

1. Lycopene Bio-availability

Lycopene is a carotenoid abundantly found in red vegetables (Figure 1). This natural pigment displays an important role in human biological systems due to its excellent antioxidant and health-supporting functions, which show a protective effect against cardiovascular diseases, hypertension, cancers, and diabetes [1][2].
Figure 1. Representation of bio-availability and functions of lycopene in humans as well as products from foods, pharmaceutical and cosmetic products.
Furthermore, studies have shown the potential anticancer activity of lycopene, suggesting that its consumption may prevent prostate, esophagus, stomach, colorectal, pancreas, breast, and cervix cancers. However, the literature has not determined the cause–effect relationship and how the consumption of lycopene-rich food decreases cancer risk [3][4][5][6].
Lycopene is an organic molecule whose molecular formula is C40H56 and molecular weight is 536.85 g·mol−1. It is insoluble in water but soluble in some organic solvents [7][8]. Its molecular structure contains 13 double bonds, 11 of which are conjugated and provide characteristics for lycopene’s antioxidant activity and strong red color [7][9].
These double bonds are affected by the action of oxidants, and can be damaged by light, acid, and heat, which destroy or rearrange the structure of lycopene to different spatial cis configurations from all-trans-isomers [10]. These effects may deteriorate and/or lead to the loss of lycopene bioactivity [5][10]. The latter seems to depend on several factors, including lycopene content, the complex composition of food, and particle size consumed in the digestive process [4].
All-trans-lycopene (Figure 2) is interesting for industrial use in food and pharmaceuticals because of its high stability compared to other isomers of lycopene. All-trans-lycopene presents higher color intensity than cis isomers due to its low extinction coefficient. In nature, the trans-lycopene configuration has better stability compared with the cis isomer [3]. Nevertheless, the industry has also demonstrated interest in cis-lycopene structures (Figure 2) because they seem to have better bio-availability when compared to the all-trans isomer and can also prevent breast and prostate cancer [11][12][13].
Figure 2. Representation of cis and trans-lycopene structure. Data deposited in computer by PubChem [14] and converted to 2D with ACD/Labs [15].
The nutritional efficacy and industrial applicability of lycopene is limited due to its insolubility in water. On the other hand, nano-emulsified or micro-encapsulated lycopene, as well as lycopene nanoparticles, have demonstrated great in vitro bio-accessibility throughout the liberation of lycopene content from the nanostructure [3][11][16][17]. Therefore, micro- or nano-encapsulated lycopene may both overcome and avoid the problems related to lycopene structure and can present opportunities for the use of this potent antioxidant.

2. Production and Extraction Process

Several processes for the production and extraction of carotenoids such as lycopene have been proposed (Table 1). The most used method for extraction is solvent extraction and supercritical fluid extraction—SFE (supercritical CO2) [3][18][19]—and for production the most used is biosynthesis in a reactor or flask fermentation using biological strains, such as bacteria and yeasts [1][8][20][21]. Regardless of the method used, the lycopene extracts obtained have a red color, and their color intensity depends on lycopene concentration in the extraction media [22].
Table 1. Type of lycopene obtained from different extraction methods and biosynthesis from 2017 to 2021.
Technique Solvent/Mobile Phase/Flow Rate Device Temperature/

Pressure/Time/

V/Hz/rpm		 Lycopene Source/

Carotenoid Extracted		 References
Extraction Hexane Soxhlet extractor 37 °C/6 h Tomato peel and seed/

cis and trans-lycopene		 [19]
* SC-CO2/1 mL·min−1 SFT 110 extractor 40 and 80 °C/30 and 50 MPa/30, 45, 60, 90, 120, 180, 240 min
Hexane/Acetone/Ethanol (2:1:1) Vortex 2 h
Washed in 0.1 M NaCl     Tomato peel and seed/

Lycopene and β-carotene		 [22]
OH pre-treatment   55 °C/1 min
Water/Ethanol (70%)

(1:6 w/v) &		 Thermal extraction 55 °C/15 min
Water/Ethanol (1:6 w/v) & + OH solution
OH application Ohmic heating (OH) technology

(6–11 V·cm−1)

pre-treatment		 0–100 °C/30 min/

60–280 V/

25 kHz
* SC-CO2
Bioreactor


(37 °C, pH = 7.2, 48 h)
Escherichia coli


FA03-PM
Glucose
Glucose + oleic acid + yeast extract
Glucose + waste cooking oil + yeast extract
Lactic acid
Flask fermentation


(120 h)
B. trispora

NRRL 2895 (+) and N6 (−) Lycopene and

β-carotene		 [8]
* SC-CO2 = Supercritical Carbon Dioxide; (w/v) & = sample weight/solvent volume.
High temperatures (above 80 °C), light, oxygen, and exposure time may degrade lycopene, while the type of solvent can increase the isomerization from all-trans-lycopene to cis-lycopene. Acetone, for example, is one of the best solvents for extracting lycopene from fresh material once it provides better solubilization of the lipophilic intracellular content [26].
The use of electric processes to extract carotenoids from food has been successfully applied, with the advantage of promoting a selective extraction and improving carotenoid bio-availability [22]. Although the effects of electric processes on carotenoids are still unknown, applying low voltages could reduce the risk of damage to their structures [26]. These results have ushered in new studies about the factors that influence degradation, as well as “green” methods of lycopene recovery.
Supercritical CO2 is a technique that significantly impacts lycopene extraction, as it is considered an environmentally friendly method when compared with those that use solvents and lower temperatures [1][18][19][26]. The bacteria and yeasts introduced into bioreactors and the species Escherichia coli, Blakeslea trispora, and Saccharomyces cerevisiae stood out for lycopene production [8][20][21]. These microorganisms consume glucose, lactic acid, and fatty acids as nutrients at a temperature of around 30 °C, producing β-carotene and lycopene as final products [1][21].
Therefore, biosynthesis methods followed by ultrasound or enzymatic lysis to damage the cell membrane before supercritical fluid extraction could be an alternative to obtaining lycopene using a clean methodology.
It is noteworthy that the extraction methods applied in obtaining lycopene from red guava are patented methodologies and, for this reason, these methods are not mentioned in Table 1.

3. Lycopene Bio-accessibility and Bio-availability—Novel Technologies

Carotenoids can offer numerous health benefits when consumed consistently (Table 2). Nevertheless, for this purpose, there must be a first release from the food matrix followed by carotenoid diffusion into oil droplets. The bile salts help to create micelles to assist the digestion of lipid forms, converting them in free fatty acids, mono- and diacylglycerides, lysophospholipids, and free cholesterol [28]. The harsh conditions throughout the absorption and assimilation process might conduct lycopene degradation due to exposure to pH changes, increased temperature, and oxidation [29]. Consequently, bio-availability and absorption are much lower than water-soluble molecules [30].
Table 2. Recent examples of methods used to encapsulate lycopene from 2017 to 2021.
Delivery System Encapsulation Method Results References
β-cyclodextrins A mixture of methylene chloride solution of lycopene with ethanol at 37 °C. Higher stability against oxidizing agents (AAPH and H2O2). [31]
β-cyclodextrins Lycopene inclusion complexes with β-cyclodextrin were prepared by

the precipitation method.		 Increased thermal stability, photostability, and antioxidant activity. [32]
Nanoliposomes Sonication of lycopene, soybean phosphatidylcholine, cholesterol, and

aqueous solution.		 Neuronal protection against cerebral ischemia/reperfusion.

Improved therapeutic efficacy and attenuated the cardiotoxicity of the chemotherapy drug doxorubicin.		 [33]
Phospholipid nanoliposomes Nanospheres of phospholipids with lycopene produced by evaporation and nanoliposomes produced by sonication with the presence of buffer and

recovered by centrifugation.		 Enhanced antioxidant activity.

Prevented reactive oxygen species-induced kidney tissue damage.		 [34]
Double-loaded

liposomes		 Lycopene, β-cyclodextrins encapsulated with soy lecithin and cholesterol. Prolonged-release.

Improvement of lycopene solubility.

Cardioprotective activity tested

in vivo.		 [35]
Oil-in-water

nano-emulsions		 Octenyl succinate anhydride-modified starch mixed with lycopene using high-pressure homogenization and medium-chain triglycerides as carrier oils. Stable nano-emulsions system with potential application for

functional foods.		 [2]
Oil-in-water

emulsions		 Emulsion of water, pure whey isolate, citric acid, triglycerides, and lycopene created with pressure homogenizer. Increased lycopene bio-accessibility.

System critical for the delivery of

lipophilic bioactive compounds in

functional drinks.		 [36]
Nanodispersions Homogenization of lycopene dissolved in dichloromethane, aqueous phase,

and Tween 20.		 Small-size lycopene nanodispersions.

Good stability for application in

beverage products.		 [37]
/1 L·min−1 SFT 110 extractor
Feed emulsions Homogenization of tomato powders, maltodextrin, and gum Arabic in aqueous solution and encapsulation made

by spray-drying.		60 °C and 40 MPa/

30, 45, 60, 90, 120,

180, 240 min		 Increased lycopene stability.Tomato peel and seed/

Highest 		 [cis-lycopene content 38][23]
Hexane Soxhlet extractor/0.22 µm hydrophobic PTFE 12 h Tomato peel and seed/

Lycopene		 [24]
Solid lipid

nanoparticles (SLN)		 Lycopene-loaded solid lipid nanoparticles using Precirol® ATO 5, Compritol® 888 ATO, and myristic acid by

hot homogenization.		 Stable after 2 months in

an aqueous medium (4 °C).		 Olive oil Maceration (15 to 150 min)/Magnetic stirrer/Box–Behnken 40–80 °C/

200–400 rpm
[39]
Solid lipid

nanoparticles (SLN)		 Cold homogenization technique with glyceryl monostearate and lycopene. Gel with a promising antioxidant therapy in periodontal defects. [40] Methanol/Ethyl acetate/

Petroleum ether (1:1:1, v/v/v)		     Tomato peel and seed from 10 varieties/

Solid lipid

nanoparticles (SLN)		 Homogenization-evaporation technique of lycopene-loaded SLN with different ratios of biocompatible Compritol® 888 ATO

and gelucire.		 Particles showed in vitro

anticancer activity.		Lycopene, β-carotene, and lutein [25]
[41] 30% Methanolic potassium hydroxide   Room temperature

Nanostructure lipid carriers (NLCs)/6 h
Ultrasonication of lycopene with

Tween 80 and Poloxamer 188.		 Enhanced oral bio-availability.

Increased cytotoxicity against human breast tumor cells.		 [42] Saturated saline solution/

Diethyl ether/

Distilled water		 Washed  
Nanostructure lipid carriers (NLCs) Homogenization and ultrasonication method (aqueous phase with Tween 80, lecithin, and lycopene). Increased lycopene aqueous solubility.

Improved solubility masking

tomato aftertaste.

Increased homogeneity of fortified orange drink.		 [43] Dry over anhydrous

sodium sulfate		 Rotary evaporator
Nanostructure lipid carriers (NLCs)

R-124		 Emulsion created with lycopene, a lipid mixture, Tween 80 followed by pressure homogenization.35 °C
Biphasic release pattern with fast release initially and a slower afterward. [6]   PEF (pre-treatment)1,3; 5 kV·cm−1/0.012 kJ·kg−1, 0.160 kJ·kg−1, 0.475 kJ·kg−1/10 Hz/20 μs 20 ± 2 °C Tomato peels/

Whey protein isolate nanoparticles Lycopene loaded whey protein

isolate nanoparticles.		cis Enhance the oral bio-availability of lycopene.

Controlled release.		 and all trans-lycopene [26]


Facilitated absorption through the lymphatic pathway. [17] Acetone (1:40 w/v) & Extraction flask 25 °C/0–24 h

/160 rpm
Gelatin nanofibers A mixture of gelatin from bovine skin and tomato extract is used in electrospinning. Better retention of lycopene.

Better antioxidant activity during

14-days storage.		 [44] Ethyl lactate (1:40 w/v) &
Ionic gelation Lycopene watermelon concentrate mixed with sodium alginate or pectin.

Encapsulation by dipping in CaCl2 and drying under vacuum.		 More stable lycopene-rich beads.

Good application as natural colorants/antioxidants in different types of food products.		 [45] Ethyl acetate Thermal extraction 75 °C/1 or 2 h Plum tomato peels/

cis
Nano-encapsulation CPCs (Chlorella pyrenoidosa cells)

loaded with lycopene into a complex

nutraceutical and exogenous.		 and  Feasibility of lycopene encapsulation in the CPCs.

Combined the activities of both

materials.

Novel nutraceuticals to reduce cellular oxidative stress.		all trans-lycopene [27]
[10] Ultrasounds Approximately 0 °C/30 min
Nano-emulsion Lycopene from guava on nanoemulsifying system of natural oils. Lycopene nano-emulsion with

high stability.

Significant inhibition of edema formation, suggesting a potential candidate for anti-inflammatory therapy.		 [16] Magnesium carbonate (20%) (sample/solution 1:1) Orbital shaker 25 °C/2 h Tomato peels/

Lycopene		 [3]
Lipid-core Nanocapsules Nano-encapsulation process mixed lycopene extract from guava with polycaprolactone polymer in acetone sorbitan monostearate. The nanostructure was cytotoxic against cancer cells (human breast adenocarcinoma line MCF-7). [12] n-hexane/Acetone (3:1)
NanoparticleUltrasonic 50 °C/30 min (10 times)
Polymer nanoparticle fucan-coated based on acetylated cashew gum and lycopene extract from guava. Promising results for applicability in hydrophobic compounds carrying systems as lycopene with cytotoxic effect on the breast cancer cell. [11]   Centrifuge 10 °C/10 min
Microencapsulation Microencapsulation of lycopene from

tomato peels by complex coacervation

and freeze-drying.		 The fine orange-yellow powder could be micro-encapsulated as stable

lycopene applied to the food industry with properties against metabolic syndrome.		 [3]   Reduced volume 40 °C/Low pressure
Technique Substrate Device Biological Strain Carotenoid Produced References
Biosynthesis Isopropyl-β-d-thiogalactoside (IPTG)

as inducer		 Shaking flask

(37 °C and 200 rpm)		 E. coli Lycopene [20]
Glucose Shake flask

(30 °C, 300–600 rpm)		 S. cerevisiae Lycopene [21]
Glucose + Glycerol Shake flask

(37 °C, pH = 7.2, 48 h)		 Escherichia coli

R122		 Lycopene [1]
Glucose
Oleic acid
Strategies have been created to control problems related to the practical application of lycopene, which is strongly restricted due to its high sensitivity when exposed to light, oxygen, and heat, as well as contact with metal ions, besides processing conditions and low water solubility [11][46]. Encapsulation is one of the techniques that frequently uses oligosaccharides, such as cyclodextrins, to associate compounds in a hydrophobic core, while on the outside it forms a hydrophilic shell. The encapsulation protects lycopene from degradation and isomerization besides increasing its solubility in aqueous environments [46]. A report on the lycopene/α- and β-CD complexes pointed that both could provide stable associations in water with profound differences in structure [47]. Maltodextrins were used during tomato processing to powders aiming to increase lycopene stability [48]. Respective delivery systems have been created to enhance lycopene bio-availability and absorption rates (Figure 1) in the gastrointestinal environment [37][49]. During digestion, carotenoids are assimilated with other lipids into mixed micelles containing bile salts and phospholipids, which perform as carriers to solubilize the carotenoids and transport them to the zone of maximum absorption in the intestine. Incorporating lycopene into the oil phase of emulsions is an alternative to protect it from oxidation and chemical degradation, providing better bio-availability and prolonging shelf-life [28]. Nano-emulsions have been reported to be suitable delivery systems with favorable results for the encapsulation of low-solubility compounds, such as lycopene [46]. Recent works have shown efficient delivery systems for lycopene: its incorporation in oil-in-water emulsions for orange beverages [50], lycopene encapsulated in isolate-Xylo-oligosaccharide protein conjugates made by Maillard reaction [49], and oil-in-water emulsions with long- to short-chain triglycerides [37]. The literature also reports the formation of liposomes and nanoliposomes, which are spherical vesicles created with a concentric phospholipid bilayer of hydrophilic center. It was reported that lycopene tends to be entrapped in the hydrophobic bilayer, enhancing bio-accessibility when exposed to the gastrointestinal tract and with increased antioxidant capacity compared with the free form [51][52]. Oil–water nano-emulsions are nanoparticles dispersed in heterogeneous systems with an inner lipidic and an external aqueous phase stabilized by one or two surfactants. Unlike the nano-emulsions, lipid nanoparticles have an internal solid lipid phase since these nanoparticles are totally or mainly composed of solid lipids at room temperature. Such a solid matrix allows the controlled release of the encapsulated molecules and protects them from degradation while increasing the long-term stability of the system [53]. Lycopene-loaded SLNs demonstrated stability in an aqueous medium for two months, producing an applicable system for future in vivo trials in nutraceutical industries [39]. The encapsulation in SLN showed an improvement in lycopene oral delivery, and an ex vivo assessment determined that this carotenoid had better permeation besides causing more cytotoxicity against breast cancer cells [42]. Lycopene loaded into nanostructured lipid carriers (NLC) composed of Eumulgin SG, orange wax, and rice bran oil, employing high pressure in homogenization process, showed chemical stability and delayed degradation when put into cold storage [54]. Moreover, lycopene nano-emulsions have provided more thermal stability for lycopene and significantly inhibited edema formation. For this reason, these nanoparticles may be considered to be a potential candidate for anti-inflammatory therapy [16]. Lipid-core nanocapsules of lycopene, in turn, optimized stability for 7 months at 5 °C storage, and improved its toxicity against breast cancer cells. The nanocapsules also inhibited the production of intracellular peroxyl radicals in human microglial cells and maintained the membrane integrity of erythrocytes, highlighting its potential to be employed in cancer treatment [12]. Lycopene encapsulated in polymeric nanoparticles showed high anti-tumor potential, with cytotoxicity against cancer cells at low concentrations and no toxicity against Galleria mellonella. Additionally, nanoparticles with sizes of 162.10 ± 3.21 nm were efficient with a passive mechanism of permeability for targeting tumor tissues [11]. On the other hand, lycopene powder produced by complex coacervation and freeze-drying after microencapsulation had promising results as a biopolymeric composite with inhibitory effect potential on α-amylase associated with metabolic syndrome, and demonstrated high antioxidant activity in formulations [3].

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