Usutu virus in Europe: Comparison
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Please note this is a comparison between Version 1 by Tatjana Vilibic-Cavlek and Version 2 by Felix Wu.

Usutu virus (USUV) is an emerging arbovirus isolated in 1959 (Usutu River, Swaziland). Previously restricted to sub-Saharan Africa, the virus was introduced in Europe in 1996. While USUV has received little attention in Africa, the virus emergence has prompted numerous studies with robust epidemiological surveillance programs in Europe. Epizootics and genetic diversity of USUV in different bird species as well as detection of the virus in mosquitoes suggest repeated USUV introductions into Europe with endemization in some countries. The zoonotic potential of USUV has been reported in a growing number of human cases. Clinical cases of neuroinvasive disease and USUV fever, as well as seroconversion in blood donors were reported in Europe since 2009. Since spreading trends of USUV are likely to continue, continuous multidisciplinary interventions ('One Health' concept) should be conducted for monitoring and prevention of this emerging arboviral infection.

  • Usutu virus
  • Epidemiology
  • Europe
  • "One Health"

1. Introduction

Note：All the information in this draft can be edited by authors. And the entry will be online only after authors edit and submit it.

Usutu virus (USUV) is an emerging arbovirus that belongs to the family Flaviviridae, genus Flavivirus, Japanese encephalitis serocomplex. Similar to other flaviviruses, USUV is a spherical, small, enveloped virus with a single stranded positive-sense RNA genome of ~12 kb. Phylogenetic analyses of the NS5 gene have shown that USUV strains clustered into eight genetic lineages: three African (Africa 1–3) and five European (Europe 1–5) [1]. In nature, USUV is maintained in a bird–mosquito–bird cycle, however the virus or antibodies detection were reported sporadically in humans, horses and other mammals (Figure 1).

USUV was isolated in 1959 from Culex neavei mosquito caught near the Usutu River in Swaziland [2]. After that, the virus was confined to Africa with only few human cases with fever, rash and jaundice reported [3]. In Africa, the USUV host range includes mosquitoes, birds, equids and dogs [4][5][4,5]. The virus was also isolated and nucleotide sequenced from five asymptomatic small mammals in Senegal that belong to two rodent species (black rat; Rattus rattus and multimammate rat; Mastomys natalensis) and a single species of shrew (Crocidura sp.) (Figure 1) [6].

A retrospective analysis of archived tissue samples originating from a bird die-off showed that USUV emerged in Europe in 1996 (Tuscany region, Italy), five years before the advent of USUV-associated bird deaths in Austria which has been generally assumed as the starting point of the virus spread in Europe [7]. In 2001, the virus caused the first large outbreak in several bird species in the region of Vienna (Austria) [8]. In the following years, continuous geographic expansion of the USUV in Europe has been shown by reports of epizootics or small outbreaks as well as serologic detection in different wild and captive bird species [9][10][11][12][9–12]. In addition, the virus was detected in different native (mainly Culex pipiens) and invasive mosquito species (Aedes albopictus, Ae. japonicus) [13][14][15][16][17][18][13–18]. USUV RNA was also found in bats (Pipistrellus pipistrellus) in Germany and Belgium [19][20][19,20]. Moreover, USUV antibodies were sporadically detected in horses [21][22][23][21–23], dogs [24], squirrels [25], wild boar, roe deer [26] and lizards [27] (Figure 1), expanding the USUV host range (Figure 1), however these species are considered incidental hosts.

Figure 1. Usutu virus (USUV) transmission cycle involves birds (amplifying hosts) and mosquitoes (vectors). Infection can be transmitted to humans and horses which are generally considered incidental or “dead-end” hosts. USUV isolation/detection (black symbols) and serologic evidence (blue symbols) were reported in different animal species, expanding the incidental host range.

 

The zoonotic potential of USUV has been reported in a limited number of human cases. Few cases of neuroinvasive disease and USUV fever, as well as seroconversion in blood donors were reported in Europe since 2009 [11][28][29][30][31][32][33][11,28–33]. Phylogenetic analyses showed that the majority of USUV strains detected in humans, birds and mosquitoes belong to European USUV lineages, however several reports indicated the presence of African lineages as well [18][34][35][36][18,34–36].

The USUV diversity in Europe appeared in the last decade, however, the phylogenies suggest a long-term virus circulation in this region [37]. The presence of USUV was documented by virus isolation/detection or serologically in Austria, Belgium, Croatia, the Czech Republic, France, Germany, Greece, Hungary, Italy, the Netherlands, Poland, Serbia, Slovakia, Spain, Switzerland and the United Kingdom.

The emergence and geographic distribution of USUV infections in European countries is presented in Table 1 and Figure 2.

Table 1. Emergence of Usutu virus (USUV) in Europe: clinical cases/seroconversion/RNA detection/serologic evidence. In Europe, USUV emerged in 1996 in the Tuscany region (Italy) when it was detected by retrospective analysis of archived tissues from dead birds. In mosquitoes, USUV was discovered for the first time in 2006 (Spain) in a pool of Cx. pipiens. Seroconversion in horses was reported in 2008 (Italy), following the first two human clinical cases in 2009. In 2013, the virus was detected in two dead bats (Pipistrellus pipistrellus) in Germany. Until 2018, USUV infections were reported in 16 European countries.

Country Pathogens 09 00699 i001 Pathogens 09 00699 i002 Pathogens 09 00699 i003 Pathogens 09 00699 i004 Pathogens 09 00699 i005Reference
Austria2016*2001 2017 [8][17][37][38][39]
Belgium 2012 20162017[1][20][40]
Croatia2012201820112016 [15][22][30][32]
Czech Republic 2004 2013 [41][42]
France20162015 2015 [31][43][44]
Germany2012*2011 20102013[9][13][19][45]
Greece 2010   [46]
Hungary20182005   [34][47]
Italy2009199620082009 [7][21][28][29][48]
Netherlands 2016   [49]
Poland 20062012  [50][51]
Serbia2015201220092014 [52][53][54]
Slovakia 2010   [23][55]
Spain 201120112006 [56][57][58][59]
Switzerland 2006   [60]
United Kingdom 2001   [61]

                

Pathogens 09 00699 i006Clinical cases/RNA detection/seroconversion; Pathogens 09 00699 i007serologic evidence; *asymptomatic blood donors/blood donations.

 

Figure 2. Geographic distribution of Usutu virus in Europe (clinical cases/RNA detection/seroconversion/serologic evidence).

2. Conclusion

Circulation of different European and African USUV lineages (Table 2, Figure 3), suggests multiple introduction events to Europe from Africa. The Europe 2 lineage is the most commonly detected USUV lineage in the European countries [16][32][62][63][16, 32, 90, 92]. The Europe 3 USUV lineage was predominantly circulating in Southern Belgium in 2017 and 2018 [20]. Recent studies from Germany [64][63] and the Netherlands [65][84] showed an increase in the USUV Africa 3 lineage detection during the same period. The genetic diversity of European lineages is most likely shaped by enzootic maintenance (in situ evolution) rather than by extensive migration, while African lineages are driven mostly by extensive migration and repeated introduction of viral variants from different geographic origins [37]. In addition to data from the published articles, the sequences retrieved from the GenBank suggest for certain European countries broader host range as well as USUV lineage diversity within a specific host.

 

Table 2. Molecular epidemiology of Usutu virus (USUV) in Europe. USUV Europe 2 lineage is the most prevalent genetic lineage detected in birds, mosquitoes and humans. In birds, Europe lineage 1, 3-5 and Africa 2 and 3 lineages were detected as well. Europe 3 and 4 and Africa 2 and 3 lineages were detected in mosquitoes. In bats, only Europe 3 lineage was documented so far.                                                      

Country Pathogens 09 00699 i008 Pathogens 09 00699 i009 Pathogens 09 00699 i010 Pathogens 09 00699 i011Reference
AustriaEurope 2*

Africa 3*		Europe 1,2

Africa 3		Europe 2** [34][35][64][66]
Belgium Europe 1,3

Africa 3		Europe 3Europe 3[20][36][64][66]
CroatiaEurope 2Europe 2Europe 2 [32]
Czech Republic Europe 1,2,3

Africa 3		Europe 2 [18][42]
FranceAfrica 2Europe 3Europe 2

Africa 2,3		 [31][44]
GermanyEurope 3Europe 2,3,5

Africa 2,3

Africa 3-like		Europe 3

Africa 3		Europe 3[9][19][64][67][68][69]
HungaryEurope 2Europe 1,2  [34][39][47][70]
ItalyEurope 1,2*,3*,4*Europe 2,4Europe 2,4 [16][29][64][71][72]
NetherlandsEurope 3Europe 3

Africa 3		  [73][74]
Serbia  Europe 1,2 [75][76]
Slovakia  Europe 2 [55]
Spain Africa 2Africa 2 [1][77]
Switzerland Europe 1  [60]

                   

*Blood donors/donations; **based on nucleotide sequences retrieved from the GenBank.

                                               

Figure 3. Neighbor-Joining tree of USUV sequences based on 495 nucleotides of the NS5 gen illustrating phylogenetic and host diversity of USUV strains detected in European countries. Taxon information includes the GenBank accession number, country in which the virus was detected, host and isolation/detection year. Colour codes for different hosts are shown in the upper left corner. USUV genetic lineages are indicated on the right. Scale bar indicates the mean number of nucleotide substitutions per site.

 

The integrated veterinary and human surveillance system ('One Health') based on the viruses detection in mosquitoes, migratory and resident birds as well as horses and poultry was proved to be useful for estimating the public health risk for other flaviviruses such as WNV. This enables the effective and timely control of the diseases in humans [78][79][80][112-114]. Since spreading trends of USUV are likely to continue, continuous multidisciplinary interventions in accordance with the 'One Health' concept, should be conducted to increase the awareness of USUV and implement appropriate monitoring and prevention methods for this emerging arboviral infection.

References

  1. Cadar, D.; Lühken, R.; van der Jeugd, H.; Garigliany, M.; Ziegler, U.; Keller, M.; Lahoreau, J.; Lachmann, J.; Becker, N.; Kik, M.; Oude Munnink, B.B.; Bosch, S.; Tannich, E.; Linden, A.; Schmidt, V.; P Koopmans, M., Rijks, J., Desmecht, D.; H Groschup, M.; Reusken, C.; Schmidt-Chanasit, J. Widespread activity of multiple lineages of Usutu virus, western Europe, 2016. Euro Surveill. 2016, 22, 30452; DOI:10.2807/1560-7917.ES.2017.22.4.30452.
  2. Williams, M.C.; Simpson D.I.; Haddow A.J.; Knight E.M. The isolation of West Nile Virus from man and of Usutu virus from the bird-biting mosquito Mansonia aurites (Theobald) in the Entebbe area of Uganda. Ann. Trop. Med. Parasitol. 1964, 58, 367-374; DOI:10.1080/00034983.1964.11686258.
  3. Nikolay, B.; Diallo, M.; Boye C.S.; Sall A.A. Usutu virus in Africa. Vector Borne Zoonotic Dis. 2011, 11, 1417-1423; DOI:10.1089/vbz.2011.0631.
  4. Durand, B.; Haskouri, H.; Lowenski, S.; Vachiery, N.; Beck, C.; Lecollinet, S. Seroprevalence of West Nile and Usutu viruses in military working horses and dogs, Morocco, 2012: dog as an alternative WNV sentinel species? Epidemiol Infect. 2016, 144(9), 1857-1864; DOI:10.1017/S095026881600011X.
  5. Ben Hassine, T.; De Massis, F.; Calistri, P.; Savini, G.; Bel Haj Mohamed, B.; Ranen, A.; Di Gennaro, A.; Sghaier, S.; Hammami, S. First detection of co-circulation of West Nile and Usutu viruses in equids in the south-west of Tunisia. Transbound Emerg Dis. 2014, 61(5), 385-389; DOI:10.1111/tbed.12259.
  6. Diagne, M.M.; Henriette, M.; Ndione, D.; Di Paola, N.; Fall, G.; Pouwedeou Bedekelabou, A.; Mbacké Sembène, P.; Faye, O.; Marinho de Andrade Zanotto, P.; Sall A.A. Usutu virus isolated from rodents in Senegal. Viruses. 2019, 11(2), 181; DOI:10.3390/v11020181.
  7. Weissenböck, H.; Bakonyi, T.; Rossi, G.; Mani, P.; Nowotny N. Usutu virus, Italy, 1996. Emerg Infect Dis. 2013, 19, 274-277; DOI:10.3201/eid1902.121191.
  8. Weissenböck, H.; Kolodziejek, J.; Url, A.; Lussy, H.; Rebel-Bauder, B.; Nowotny, N. Emergence of Usutu virus, an African mosquito-borne flavivirus of the Japanese encephalitis virus group, central Europe. Emerg Infect Dis. 2002, 8, 652-656; DOI:10.3201/eid0807.020094
  9. Becker, N.; Jöst, H.; Ziegler, U.; Eiden, M.; Höper, D.; Emmerich, P.; Fichet-Calvet, E.; Ehichioya, DU.; Czajka, C.; Gabriel, M.; Hoffmann, B.; Beer, M.; Tenner-Racz, K.; Racz, P.; Günther, S.; Wink, M.; Bosch, S.; Konrad, A.; Pfeffer, M.; Groschup, M.H.; Schmidt-Chanasit, J. Epizootic emergence of Usutu virus in wild and captive birds in Germany. PLoS One. 2012, 7, e32604; DOI: 10.1371/journal.pone.0032604.
  10. Rouffaer, L.O.; Steensels, M.; Verlinden, M.; Vervaeke, M.; Boonyarittichaikij, R.; Martel, A.; Lambrecht, B. Usutu virus epizootic and Plasmodium coinfection in Eurasian blackbirds (Turdus merula) in Flanders, Belgium. J Wildl Dis. 2018, 54, 859-862; DOI:10.7589/2017-07-163.
  11. Zannoli, S.; Sambri V. West Nile virus and Usutu virus co-circulation in Europe: epidemiology and implications. Microorganisms. 2019, 7, e184; DOI:10.3390/microorganisms7070184.
  12. Benzarti, E.; Linden, A.; Desmecht, D.; Garigliany, M. Mosquito-borne epornitic flaviviruses: an updateand review. J Gen Virol. 2019, 100, 119-132, DOI:10.1099/jgv.0.001203.
  13. Jöst, H.; Bialonski, A.; Maus, D.; Sambri, V.; Eiden, M.; Groschup, M.H.; Günther, S.; Becker, N.; Schmidt-Chanasit, J. Isolation of Usutu virus in Germany. Am J Trop Med Hyg. 2011, 85, 551-553; DOI:10.4269/ajtmh.2011.11-0248.
  14. Calzolari, M.; Gaibani, P.; Bellini, R.; Defilippo, F.; Pierro, A.; Albieri, A.; Maioli, G.; Luppi, A.; Rossini, G.; Balzani, A.; Tamba, M.; Galletti, G.; Gelati, A.; Carrieri, M.; Poglayen, G.; Cavrini, F.; Natalini, S.; Dottori, M.; Sambri, V.; Angelini, P.; Bonilauri, P. Mosquito, bird and human surveillance of West Nile and Usutu viruses in Emilia-Romagna Region (Italy) in 2010. PLoS One. 2012, 7, e38058; DOI:10.1371/journal.pone.0038058.
  15. Klobucar, A.; Benic, N.; Krajcar, D.; Kosanovic-Licina, M.L.; Tesic, V.; Merdic, E.; Vrucina, I.; Savic, V.; Barbic, L.; Stevanovic, V.; Pem-Novosel, I.; Vilibic-Cavlek, T. An overview of mosquitoes and emerging arboviral infections in the Zagreb area, Croatia. J Infect Dev Ctries. 2016, 10, 1286-1293; DOI:10.3855/jidc.7988.
  16. Calzolari, M.; Chiapponi, C.; Bonilauri, P.; Lelli, D.; Baioni, L.; Barbieri, I.; Lavazza, A.; Pongolini, S.; Dottori, M.; Moreno, A. Co-circulation of two Usutu virus strains in Northern Italy between 2009 and 2014. Infect Genet Evol. 2017, 51, 255-262; DOI:10.1016/j.meegid.2017.03.022.
  17. Camp, J.V.; Kolodziejek, J.; Nowotny, N. Targeted surveillance reveals native and invasive mosquito species infected with Usutu virus. Parasit Vectors. 2019, 12, 46; DOI:10.1186/s13071-019-3316-z.
  18. Hönig, V.; Palus, M.; Kaspar, T.; Zemanova, M.; Majerova, K.; Hofmannova, L.; Papezik, P.; Sikutova, S.; Rettich, F.; Hubalek, Z.; Rudolf, I.; Votypka, J.; Modry, D.; Ruzek, D. Multiple lineages of Usutu virus (Flaviviridae, Flavivirus) in blackbirds (Turdus merula) and mosquitoes (Culex pipiens, Cx. modestus) in the Czech Republic (2016-2019). Microorganisms. 2019, 7, e568; DOI:10.3390/microorganisms7110568.
  19. Cadar, D.; Becker, N.; Campos Rde, M.; Börstler, J.; Jöst, H.; Schmidt-Chanasit, J. Usutu virus in bats, Germany, 2013. Emerg Infect Dis. 2014, 20, 1771-1773; DOI:10.3201/eid2010.140909.
  20. Benzarti, E.; Sarlet, M.; Franssen, M.; Cadar, D.; Schmidt-Chanasit, J.; Rivas, J.F.; Linden, A.; Desmecht, D.; Garigliany, M. Usutu virus epizootic in Belgium in 2017 and 2018: evidence of virus endemization and ongoing introduction events. Vector Borne Zoonotic Dis. 2020, 20, 43-50; DOI:10.1089/vbz.2019.2469.
  21. Savini, G.; Monaco, F.; Terregino, C.; Di Gennaro, A.; Bano, L.; Pinoni, C.; De Nardi, R.; Bonilauri, P.; Pecorari, M.; Di Gialleonardo, L.; Bonfanti, L.; Polci, A.; Calistri, P.; Lelli, R. Usutu virus in Italy: an emergence or a silent infection? Vet Microbiol. 2011, 151, 264-274; DOI:10.1016/j.vetmic.2011.03.036.
  22. Barbic, L.; Vilibic-Cavlek, T.; Listes, E.; Stevanovic, V.; Gjenero-Margan, I.; Ljubin-Sternak, S.; Pem-Novosel, I.; Listes, I.; Mlinaric-Galinovic, G.; Di Gennaro, A.; Savini, G. Demonstration of Usutu virus antibodies in horses, Croatia. Vector Borne Zoonotic Dis. 2013, 13, 772-774; DOI:10.1089/vbz.2012.1236.
  23. Csank, T.; Drzewnioková, P.; Korytár, Ľ.; Major, P.; Gyuranecz, M.; Pistl, J.; Bakonyi, T. A Serosurvey of flavivirus infection in horses and birds in Slovakia. Vector Borne Zoonotic Dis. 2018, 18, 206-213; DOI:10.1089/vbz.2017.2216.
  24. Montagnaro, S.; Piantedosi, D.; Ciarcia, R.; Loponte, R.; Veneziano, V.; Fusco, G.; Amoroso, M.G.; Ferrara, G.; Damiano, S.; Iovane, G.; Pagnini, U. Serological evidence of mosquito-borne flaviviruses circulation in hunting dogs in Campania Region, Italy. Vector Borne Zoonotic Dis. 2019, 19, 142-147; DOI:10.1089/vbz.2018.2337.
  25. Romeo, C.; Lecollinet, S.; Caballero, J.; Isla, J.; Luzzago, C.; Ferrari, N.; García-Bocanegra, I. Are tree squirrels involved in the circulation of flaviviruses in Italy? Transbound Emerg Dis. 2018, 65, 1372-1376; DOI:10.1111/tbed.12874.
  26. Bournez, L.; Umhang, G.; Faure, E.; Boucher, J.M.; Boué, F.; Jourdain, E.; Sarasa, M.; Llorente, F.; Jiménez-Clavero, M.A.; Moutailler, S.; Lacour, S-A.; Lecollinet, S.; Beck, C. Exposure of wild ungulates to the Usutu and tick-borne encephalitis viruses in France in 2009-2014: evidence of undetected flavivirus circulation a decade ago. Viruses. 2019, 12, e10; DOI:10.3390/v12010010.
  27. Csank, T.; Pikalík, M.; Majláthová, V.; Majláth, I.; Pistl, J. Detection of neutralizing antibodies against Usutu virus in green lizards (Lacerta viridis). Proceedings of the Joint Czechoslovak Virology Conference 2019 and 1st SK-AT Structural Virology Meeting, Bratislava, Slovakia, 13-15 February 2019. Klempa, Nemčovicová, Černý, Tomášková, Stolt-Bergner; Biology Centre AS CR v.v.i; České Budějovice, Czech Republic, 2019. pp. 48-49.
  28. Cavrini, F.; Gaibani, P.; Longo, G.; Pierro, A.M.; Rossini, G.; Bonilauri, P.; Gerunda, G.E.; Di Benedetto, F.; Pasetto, A.; Girardis, M.; Dottori, M.; Landini, M.P.; Sambri, V. Usutu virus infection in a patient who underwent orthotropic liver transplantation, Italy, August-September 2009. Euro Surveill. 2009, 14, 19448.
  29. Pecorari, M.; Longo, G.; Gennari, W.; Grottola, A.; Sabbatini, A.; Tagliazucchi, S.; Savini, G.; Monaco, F.; Simone, M.; Lelli, R.; Rumpianesi, F. First human case of Usutu virus neuroinvasive infection, Italy, August-September 2009. Euro Surveill. 2009, 14, 19446.
  30. Vilibic-Cavlek, T.; Kaic, B.; Barbic, L.; Pem-Novosel, I.; Slavic-Vrzic, V.; Lesnikar, V.; Kurecic-Filipovic, S.; Babic-Erceg, A.; Listes, E.; Stevanovic, V.; Gjenero-Margan, I.; Savini, G. First evidence of simultaneous occurrence of West Nile virus and Usutu virus neuroinvasive disease in humans in Croatia during the 2013 outbreak. Infection. 2014, 42, 689-695. DOI:10.1007/s15010-014-0625-1.
  31. Simonin, Y.; Sillam, O.; Carles, M.J.; Gutierrez, S.; Gil, P.; Constant, O.; Martin, M.F.; Girard, G.; Van de Perre, P.; Salinas, S.; Leparc-Goffart, I.; Foulongne, V. Human Usutu virus infection with atypical neurologic presentation, Montpellier, France, 2016. Emerg Infect Dis. 2018, 24, 875-878: DOI:10.3201/eid2405.171122.
  32. Vilibic-Cavlek, T.; Savic, V.; Sabadi, D.; Peric, L.; Barbic, L.; Klobucar, A.; Miklausic, B.; Tabain, I.; Santini, M.; Vucelja, M.; Dvorski, E.; Butigan, T.; Kolaric-Sviben, G.; Potocnik-Hunjadi, T.; Balenovic, M.; Bogdanic, M.; Andric, Z.; Stevanovic, V.; Capak, K.; Balicevic, M.; Listes, E.; Savini, G. Prevalence and molecular epidemiology of West Nile and Usutu virus infections in Croatia in the 'One health' context, 2018. Transbound Emerg Dis. 2019, 66, 1946-1957; DOI:10.1111/tbed.13225.
  33. Pacenti, M.; Sinigaglia, A.; Martello, T.; De Rui, M.E.; Franchin, E.; Pagni, S.; Peta, E.; Riccetti, S.; Milani, A.; Montarsi, F.; Capelli, G.; Doroldi, C.G.; Bigolin, F.; Santelli, L.; Nardetto, L.; Zoccarato, M.; Barzon, L. Clinical and virological findings in patients with Usutu virus infection, northern Italy, 2018. Euro Surveill. 2019, 24; DOI:10.2807/1560-7917.ES.2019.24.47.1900180.
  34. Bakonyi, T.; Erdélyi, K.; Brunthaler, R.; Dán, Á.; Weissenböck, H.; Nowotny, N. Usutu virus, Austria and Hungary, 2010-2016. Emerg Microbes Infect. 2017, 6, e85; DOI:10.1038/emi.2017.72.
  35. Aberle, S.W.; Kolodziejek, J.; Jungbauer, C.; Stiasny, K.; Aberle, J.H.; Zoufaly, A.; Hourfar, M.K.; Weidner, L.; Nowotny, N. Increase in human West Nile and Usutu virus infections, Austria, 2018. Euro Surveill. 2018, 23; DOI: 10.2807/1560-7917.ES.2018.23.43.1800545.
  36. Garigliany, M.; Linden, A.; Gilliau, G.; Levy, E.; Sarlet, M.; Franssen, M.; Benzarti, E.; Derouaux, A.; Francis, F.; Desmecht, D. Usutu virus, Belgium, 2016. Infect Genet Evol. 2017, 48, 116-119; DOI:10.1016/j.meegid.2016.12.023.
  37. Engel, D.; Jöst, H.; Wink, M.; Börstler J, Bosch S, Garigliany M.M.; Jöst A.; Czajka C.; Lühken, R.; Ziegler, U.; Groschup, M.H.; Pfeffer, M.; Becker, N.; Cadar, D.; Schmidt-Chanasit, J. Reconstruction of the evolutionary history and dispersal of Usutu virus, a neglected emerging arbovirus in Europe and Africa. mBio. 2016, 7, e01938-15; DOI:10.1128/mBio.01938-15.
  38. Weissenböck, H.; Kolodziejek, J.; Fragner. K.; Kuhn, R.; Pfeffer, M.; Nowotny, N. Usutu virus activity in Austria, 2001–2002. Microbes Infect. 2003, 5, 1132-1136; DOI:10.1016/s1286-4579(03)00204-1.
  39. Weidinger, P.; Kolodziejek. J.; Bakonyi, T.; Brunthaler, R.; Erdélyi, K.; Weissenböck, H.; Nowotny, N. Different dynamics of Usutu virus infections in Austria and Hungary, 2017-2018. Transbound Emerg Dis. 2020, 67, 298-307; DOI:10.1111/tbed.13351.
  40. Chvala, S.; Bakonyi, T.; Bukovsky, C.; Meister, T.; Brugger, K.; Rubel, F.; Nowotny, N.; Weissenböck, H. Monitoring of Usutu virus activity and spread by using dead bird surveillance in Austria, 2003-2005. Vet Microbiol. 2007, 122, 237-245; DOI:10.1016/j.vetmic.2007.01.029.
  41. Meister, T.; Lussy, H.; Bakonyi,T.; Sikutová, S.; Rudolf, I.; Vogl, W.; Winkler, H.; Frey, H.; Hubálek, Z.; Nowotny, N.; Weissenböck, H. Serological evidence of continuing high Usutu virus (Flaviviridae) activity and establishment of herd immunity in wild birds in Austria. Vet Microbiol. 2008, 127, 237-248; DOI:10.1016/j.vetmic.2007.08.023.
  42. Buchebner, N.; Zenker, W.; Wenker, C.; Steinmetz, H.W.; Sós, E.; Lussy, H.; Nowotny, N. Low Usutu virus seroprevalence in four zoological gardens in central Europe. BMC Vet. Res. 2013, 9, 153; DOI:10.1186/1746-6148-9-153.
  43. Bakonyi, T.; Jungbauer, C.; Aberle, SW.; Kolodziejek, J.; Dimmel, K.; Stiasny, K.; Allerberger, F.; Nowotny, N. Usutu virus infections among blood donors, Austria, July and August 2017 - Raising awareness for diagnostic challenges. Euro Surveill. 2017, 22; DOI:10.2807/1560-7917.ES.2017.22.41.17-00644.
  44. Garigliany, M.M.; Marlier, D.; Tenner-Racz, K.; Eiden, M.; Cassart, D.; Gandar, F.; Beer, M.; Schmidt-Chanasit, J.; Desmecht, D. Detection of Usutu virus in a bullfinch (Pyrrhula pyrrhula) and a great spotted woodpecker (Dendrocopos major) in north-west Europe. Vet J. 2014, 199, 191-193; DOI:10.1016/j.tvjl.2013.10.017.
  45. Benzarti, E.; Garigliany, M.; Hauman, D.; Paternostre, J.; Linden, A.; Franssen, M.; Sarlet, M.; Cassart, D.; Desmecht, D. First evidence of fatal Usutu virus natural infections in an Anatidae, the Common Scoter (Melanitta nigra). Vector Borne Zoonotic Dis. 2019, 19, 777-780; DOI:10.1089/vbz.2019.2460.
  46. Vilibić-Čavlek, T.; Barbić Lj.; Stevanović, V.; Mlinarić-Galinović, G. Usutu virus: a novel flavivirus in Croatia. Lijec Vjesn. 2015, 137, 46-51. (In Croatian)
  47. Santini, M.; Vilibic-Cavlek, T.; Barsic, B.; Barbic, L.; Savic, V.; Stevanovic, V.; Listes, E.; Di Gennaro, A.; Savini, G. First cases of human Usutu virus neuroinvasive infection in Croatia, August-September 2013: clinical and laboratory features. J Neurovirol. 2015, 21, 92-97; DOI:10.1007/s13365-014-0300-4.
  48. Vilibic-Cavlek, T.; Savic, V.; Petrovic, T.; Toplak, I.; Barbic, L.; Petric, D.; Tabain, I.; Hrnjakovic-Cvjetkovic, I.; Bogdanic, M.; Klobucar, A.; Mrzljak, A.; Stevanovic, V.; Dinjar-Kujundzic, P.; Radmanic, L.; Monaco, F.; Listes, E.; Savini, G. Emerging trends in the epidemiology of West Nile and Usutu virus infections in Southern Europe. Front Vet Sci. 2019, 6, 437; DOI:10.3389/fvets.2019.00437.
  49. Hubálek, Z.; Halouzka, J.; Juricová, Z.; Sikutová, S.; Rudolf, I.; Honza, M.; Janková, J.; Chytil, J.; Marec, F.; Sitko, J. Serologic survey of birds for West Nile flavivirus in southern Moravia (Czech Republic). Vector Borne Zoonotic Dis. 2008, 8, 659-666; DOI:10.1089/vbz.2007.0283.
  50. Hubálek, Z.; Rudolf, I.; Čapek, M.; Bakonyi, T.; Betášová, L.; Nowotny, N. Usutu virus in blackbirds (Turdus merula), Czech Republic, 2011-2012. Transbound Emerg Dis. 2014, 61, 273-276; DOI:10.1111/tbed.12025.
  51. Rudolf, I.; Bakonyi, T.; Šebesta, O.; Mendel, J.; Peško, J.; Betášová, L.; Blažejová, H.; Venclíková, K.; Straková, P.; Nowotny, N.; Hubálek, Z. Co-circulation of Usutu virus and West Nile virus in a reed bed ecosystem. Parasit Vectors. 2015, 8, 520; DOI:10.1186/s13071-015-1139-0.
  52. Vittecoq, M.; Lecollinet, S.; Jourdain, E.; Thomas, F.; Blanchon, T.; Arnal, A.; Lowenski, S.; Gauthier-Clerc, M. Recent circulation of West Nile virus and potentially other closely related flaviviruses in Southern France. Vector Borne Zoonotic Dis. 2013, 13, 610-613; DOI:10.1089/vbz.2012.1166.
  53. Lecollinet, S.; Blanchard, Y.; Manson, C.; Lowenski, S.; Laloy, E.; Quenault, H.; Touzain, F.; Lucas, P.; Eraud, C.; Bahuon, C.; Zientara, S.; Beck, C.; Decors, A. Dual emergence of Usutu virus in common blackbirds, Eastern France, 2015. Emerg Infect Dis. 2016, 22, 2225; DOI:10.3201/eid2212.161272.
  54. Eiden, M.; Gil, P.; Ziegler, U.; Rakotoarivony, I.; Marie, A.; Frances, B.; L'Ambert, G.; Simonin, Y.; Foulongne, V.; Groschup, M.H.; Gutierrez, S.; Eloit, M. Emergence of two Usutu virus lineages in Culex pipiens mosquitoes in the Camargue, France, 2015. Infect Genet Evol. 2018, 61, 151-154; DOI:10.1016/j.meegid.2018.03.020.
  55. Roesch, F.; Fajardo, A.; Moratorio, G.; Vignuzzi, M. Usutu virus: An arbovirus on the rise. Viruses. 2019, 11, 640; DOI:10.3390/v11070640.
  56. Ziegler, U.; Jöst, H.; Müller, K.; Fischer, D.; Rinder, M.; Tietze, D.T.; Danner, K.J.; Becker, N.; Skuballa, J.; Hamann, H.P.; Bosch, S.; Fast, C.; Eiden, M.; Schmidt-Chanasit, J.; Groschup, M.H. Epidemic spread of Usutu virus in Southwest Germany in 2011 to 2013 and monitoring of wild birds for Usutu and West Nile viruses. Vector Borne Zoonotic Dis. 2015, 15, 481-488; DOI:10.1089/vbz.2014.1746.
  57. Allering, L; Jöst, H; Emmerich, P; Günther, S; Lattwein, E; Schmidt, M; Seifried, E; Sambri, V; Hourfar, K; Schmidt-Chanasit, J. Detection of Usutu virus infection in a healthy blood donor from south-west Germany, 2012. Euro Surveill. 2012; 17(50). pii: 20341.
  58. Lühken, R.; Jöst, H.; Cadar, D.; Thomas, S.M.; Bosch, S.; Tannich, E.; Becker, N.; Ziegler, U.; Lachmann, L.; Schmidt-Chanasit, J. Distribution of Usutu virus in Germany and its effect on breeding bird populations. Emerg Infect Dis. 2017, 23, 1994-2001; DOI:10.3201/eid2312.171257.
  59. Scheuch, D.E.; Schäferm M.; Eiden, M.; Heym, E.C.; Ziegler, U.; Walther, D.; Schmidt-Chanasit, J.; Keller, M.; Groschup, M.H.; Kampen, H. Detection of Usutu, Sindbis, and Batai viruses in mosquitoes (Diptera: Culicidae) collected in Germany, 2011⁻2016. Viruses. 2018, 10, E389; DOI:10.3390/v10070389.
  60. Ziegler, U.; Fast, C.; Eiden, M.; Bock, S.; Schulze, C.; Hoeper, D.; Ochs, A.; Schlieben, P.; Keller, M.; Zielke, D.E.; Luehken, R.; Cadar, D.; Walther, D.; Schmidt-Chanasit, J.; Groschup, M,H. Evidence for an independent third Usutu virus introduction into Germany. Vet Microbiol. 2016, 192, 60-66; DOI:10.1016/j.vetmic.2016.06.007.
  61. Sieg, M.; Schmidt, V.; Ziegler, U.; Keller, M.; Höper, D., Heenemann, K.; Rückner, A.; Nieper, H.; Muluneh, A.; Groschup, M.H.; Vahlenkamp, T.W. Outbreak and cocirculation of three different Usutu virus strains in Eastern Germany. Vector Borne Zoonotic Dis. 2017, 17, 662-664; DOI:10.1089/vbz.2016.2096.
  62. Llopis, I.V.; Rossi, L.; Di Gennaro, A.; Mosca, A.; Teodori, L.; Tomassone, L.; Grego, E.; Monaco, F.; Lorusso, A.; Savini, G. Further circulation of West Nile and Usutu viruses in wild birds in Italy. Infect. Genet. Evol. 2015, 32, 292–297.Cadar, D.; Maier, P.; Müller, S.; Kress, J.; Chudy, M.; Bialonski, A.; Schlaphof, A.; Jansen, S.; Jöst, H.; Tannich, E.; Runkel, S.; Hitzler, W.E.; Hutschenreuter, G.; Wessiepe, M.; Schmidt-Chanasit, J. Blood donor screening for West Nile virus (WNV) revealed acute Usutu virus (USUV) infection, Germany, September 2016. Euro Surveill. 2017, 22, 30501; DOI:10.2807/1560-7917.ES.2017.22.14.30501.
  63. Pautasso, A.; Radaelli, M.C.; Ballardini, M.; Francese, D.R.; Verna, F.; Modesto, P.; Grattarola, C.; Desiato, R.; Bertolini, S.; Vitale, N.; et al. Detection of West Nile and Usutu Viruses in Italian Free Areas: Entomological Surveillance in Piemonte and Liguria Regions, 2014. Vector Borne Zoonotic Dis. 2016, 16, 292–294.Michel, F.; Sieg, M.; Fischer, D.; Keller, M.; Eiden, M.; Reuschel, M.; Schmidt, V.; Schwehn, R.; Rinder, M.; Urbaniak, S.; Müller, K.; Schmoock, M.; Lühken, R.; Wysocki, P.; Fast, C.; Lierz, M.; Korbel, R.; Vahlenkamp, T.W.; Groschup, M.H.; Ziegler, U. Evidence for West Nile virus and Usutu virus infections in wild and resident birds in Germany, 2017 and 2018. Viruses. 2019, 11, E674. DOI:10.3390/v11070674.
  64. Daniel Cadar; Philipp Maier; Susanne Müller; Julia Kress; Michael Chudy; Alexandra Bialonski; Alexander Schlaphof; Stephanie Jansen; Hanna Jöst; Egbert Tannich; et al.Stefan RunkelWalter E. HitzlerGabriele HutschenreuterMartina WessiepeJonas Schmidt-Chanasit Blood donor screening for West Nile virus (WNV) revealed acute Usutu virus (USUV) infection, Germany, September 2016. Eurosurveillance 2017, 22, 30501, 10.2807/1560-7917.es.2017.22.14.30501.Chaintoutis, S.C.; Dovas, C.I.; Papanastassopoulou, M.; Gewehr, S.; Danis, K.; Beck, C.; Lecollinet, S.; Antalis, V.; Kalaitzopoulou, S.; Panagiotopoulos, T.; Mourelatos, S.; Zientara, S. Papadopoulos O. Evaluation of a West Nile virus surveillance and early warning system in Greece, based on domestic pigeons. Comp Immunol Microbiol Infect Dis. 2014, 37, 131-141; DOI:10.1016/j.cimid.2014.01.004.
  65. Ute Ziegler; Hanna Jöst; Kerstin Müller; D. Fischer; Monika Rinder; Dieter Thomas Tietze; Klaus-Jürgen Danner; Norbert Becker; Jasmin Skuballa; Hans-Peter Hamann; et al.Stefan BoschChristine FastMartin EidenJonas Schmidt-ChanasitMartin H. Groschup Epidemic Spread of Usutu Virus in Southwest Germany in 2011 to 2013 and Monitoring of Wild Birds for Usutu and West Nile Viruses. Vector-Borne and Zoonotic Diseases 2015, 15, 481-488, 10.1089/vbz.2014.1746.Bakonyi, T.; Erdélyi, K.; Ursu, K.; Ferenczi, E.; Csörgo, T.; Lussy, H.; Chvala, S.; Bukovsky, C.; Meister, T.; Weissenböck, H.; Nowotny, N. Emergence of Usutu virus in Hungary. J Clin Microbiol. 2007, 45, 3870-3874; DOI:10.1128/JCM.01390-07.
  66. Pia Weidinger; Jolanta Kolodziejek; Tamás Bakonyi; René Brunthaler; Károly Erdélyi; Herbert Weissenböck; Norbert Nowotny; Different dynamics of Usutu virus infections in Austria and Hungary, 2017–2018. Transboundary and Emerging Diseases 2019, 67, 298-307, 10.1111/tbed.13351.Nagy, A.; Mezei, E.; Nagy, O.; Bakonyi, T.; Csonka, N.; Kaposi, M.; Koroknai, A.; Szomor, K.; Rigó, Z.; Molnár, Z.; Dánielisz, Á.; Takács, M. Extraordinary increase in West Nile virus cases and first confirmed human Usutu virus infection in Hungary, 2018. Euro Surveill. 2019, 24, 1900038; DOI:10.2807/1560-7917.ES.2019.24.28.1900038.
  67. Hubálek, Z.; Rudolf, I.; Čapek, M.; Bakonyi, T.; Betášová, L.; Nowotny, N. Usutu virus in blackbirds (Turdus merula), Czech Republic, 2011–2012. Transbound. Emerg. Dis. 2014, 61, 273–276.Manarolla, G.; Bakonyi, T.; Gallazzi, D.; Crosta, L.; Weissenböck, H.; Dorrestein, G.M.; Nowotny, N. Usutu virus in wild birds in northern Italy. Vet Microbiol. 2010, 141, 159-163; DOI:10.1016/j.vetmic.2009.07.036.
  68. Sieg, M.; Schmidt, V.; Ziegler, U.; Keller, M.; Höper, D.; Heenemann, K.; Rückner, A.; Nieper, H.; Muluneh, A.; Groschup, M.H.; et al. Outbreak and Cocirculation of Three Different Usutu Virus Strains in Eastern Germany. Vector Borne Zoonotic Dis. 2017, 17, 662–664.Lelli, R.; Savini, G.; Teodori, L.; Filipponi, G.; Di Gennaro, A.; Leone, A.; Di Gialleonardo, L.; Venturi, L.; Caporale, V. Serological evidence of Usutu virus occurrence in north-eastern Italy. Zoonoses Public Health. 2008, 55, 361-367; DOI:10.1111/j.1863-2378.2008.01146.x.
  69. Michel, F.; Sieg, M.; Fischer, D.; Keller, M.; Eiden, M.; Reuschel, M.; Schmidt, V.; Schwehn, R.; Rinder, M.; Urbaniak, S.; et al. Evidence for West Nile Virus and Usutu Virus Infections in Wild and Resident Birds in Germany, 2017 and 2018. Viruses 2019, 11, 674.Tamba, M.; Bonilauri, P.; Bellini, R.; Calzolari, M.; Albieri, A.; Sambri, V.; Dottori, M.; Angelini, P. Detection of Usutu virus within a West Nile virus surveillance program in Northern Italy. Vector Borne Zoonotic Dis. 2011, 11, 551-557; DOI:10.1089/vbz.2010.0055.
  70. Tamás Bakonyi; Károly Erdélyi; Krisztina Ursu; Emoke Ferenczi; Tibor Csörgo; Helga Lussy; Sonja Chvala; Christiane Bukovsky; Tanja Meister; Herbert Weissenböck; et al.Norbert Nowotny Emergence of Usutu Virus in Hungary. Journal of Clinical Microbiology 2007, 45, 3870-3874, 10.1128/jcm.01390-07.Llopis, I.V.; Rossi, L.; Di Gennaro, A.; Mosca, A.; Teodori, L.; Tomassone, L.; Grego, E.; Monaco, F.; Lorusso, A.; Savini, G. Further circulation of West Nile and Usutu viruses in wild birds in Italy. Infect Genet Evol. 2015, 32, 292-297; DOI:10.1016/j.meegid.2015.03.024.
  71. Caracciolo, I.; Cardenas, E.M.; Aloise, C.; Carletti, T.; Segat, L.; Burali, M.S.; Chiarvesio, A.; Totis, V.; Avšič–Županc, T.; Mastrangelo, E.; et al. Comprehensive response to Usutu virus following first isolation in blood donors in the Friuli Venezia Giulia region of Italy: Development of recombinant NS1-based serology and sensitivity to antiviral drugs. PLoS Negl. Trop. Dis. 2020, 14, e0008156.Puggioli, A.; Bonilauri, P.; Calzolari, M.; Lelli, D.; Carrieri, M.; Urbanelli, S.; Pudar, D.; Bellini, R. Does Aedes albopictus (Diptera: Culicidae) play any role in Usutu virus transmission in Northern Italy? Experimental oral infection and field evidences. Acta Trop. 2017, 172, 192-196; DOI:10.1016/j.actatropica.2017.05.006.
  72. Carletti, F.; Colavita, F.; Rovida, F.; Percivalle, E.; Baldanti, F.; Ricci, I.; De Liberato, C.; Rosone, F.; Messina, F.; Lalle, E.; et al. Expanding Usutu virus circulation in Italy: Detection in the Lazio region, central Italy, 2017 to 2018. Eurosurveillance 2019, 24, 1800649.Pautasso, A.; Radaelli, M.C.; Ballardini, M.; Francese, D.R.; Verna, F.; Modesto, P.; Grattarola, C.; Desiato, R.; Bertolini, S.; Vitale, N.; Ferrari, A.; Rossini, I.; Accorsi, A.; Mosca, A.; Monaco, F.; Savini, G.; Prearo, M.; Mignone, W.; Chiavacci, L.; Casalone, C. Detection of West Nile and Usutu viruses in Italian free Areas: entomological surveillance in Piemonte and Liguria Regions, 2014. Vector Borne Zoonotic Dis. 2016, 16, 292-294; DOI:10.1089/vbz.2015.1851.
  73. Munnink, B.B.O.; Münger, E.; Nieuwenhuijse, D.F.; Kohl, R.; Van Der Linden, A.; Schapendonk, C.M.E.; Van Der Jeugd, H.; Kik, M.; Rijks, J.M.; Reusken, C.B.E.M.; et al. Genomic monitoring to understand the emergence and spread of Usutu virus in the Netherlands, 2016–2018. Sci. Rep. 2020, 10, 1–10.Llopis, I.V.; Tomassone, L.; Grego, E.; Silvano, F.; Rossi, L. Investigation into Usutu and West Nile viruses in ticks from wild birds in Northwestern Italy, 2012-2014. New Microbiol. 2017, 40, 56-57.
  74. Zaaijer, H.L.; Slot, E.; Molier, M.; Reusken, C.B.; Koppelman, M.H. Usutu virus infection in Dutch blood donors. Transfusion 2019, 59, 2931–2937.Caracciolo, I.; Mora-Cardenas, E.; Aloise, C.; Carletti, T.; Segat, L.; Burali, M.S.; Chiarvesio, A.; Totis, V.; Avšič-Županc, T.; Mastrangelo, E.; Manfroni, G.; D’Agaro, P.; Marcello A. Comprehensive response to Usutu virus following first isolation in blood donors in the Friuli Venezia Giulia region of Italy: Development of recombinant NS1-based serology and sensitivity to antiviral drugs. PLoS Negl Trop Dis. 2020, 14(3), e0008156; DOI:10.1371/journal.pntd.0008156.
  75. Kemenesi, G.; Buzás, D.; Zana, B.; Kurucz, K.; Krtinić, B.; Kepner, A.; Földes, F.; Jakab, F. First genetic characterization of Usutu virus from Culex pipiens mosquitoes Serbia, 2014. Infect. Genet. Evol. 2018, 63, 58–61.Grottola, A.; Marcacci, M.; Tagliazucchi, S.; Gennari, W.; Di Gennaro, A.; Orsini, M.; Monaco, F.; Marchegiano, P.; Marini, V.; Meacci, M.; Rumpianesi, F.; Lorusso, A.; Pecorari, M.; Savini, G. Usutu virus infections in humans: a retrospective analysis in the municipality of Modena, Italy. Clin Microbiol Infect. 2017, 23, 33-37; DOI:10.1016/j.cmi.2016.09.019.
  76. Petrović, T.; Šekler, M.; Petrić, D.; Vidanović, D.; Potkonjak, A.; Cvjetković, I.H.; Savić, S.; Debeljak, Z.; Lazić, G.; Ćupina, A.I.; et al. Flaviviruses at the territory of Serbia—Present situation and challenges. Arch. Vet. Med. 2018, 11, 53–70.Gaibani, P.; Pierro, A.; Alicino, R.; Rossini, G.; Cavrini, F.; Landini, M.P.; Sambri, V. Detection of Usutu-virus-specific IgG in blood donors from northern Italy. Vector Borne Zoonotic Dis. 2012, 12, 431-433; DOI:10.1089/vbz.2011.0813.
  77. Ana Vázquez; Juana Moreno; Antonio Tenorio; Jordi Figuerola; Maria Paz Sánchez-Seco; Santiago Ruiz; Antonio Magallanes; Francisca Molero; Laura Herrero; West Nile and Usutu Viruses in Mosquitoes in Spain, 2008–2009. The American Journal of Tropical Medicine and Hygiene 2011, 85, 178-181, 10.4269/ajtmh.2011.11-0042.Percivalle, E.; Sassera, D.; Rovida, F.; Isernia, P.; Fabbi, M.; Baldanti, F.; Marone, P. Usutu virus antibodies in blood donors and healthy forestry workers in the Lombardy Region, Northern Italy. Vector Borne Zoonotic Dis. 2017, 17, 658-661; DOI:10.1089/vbz.2017.2126.
  78. Rizzo, C.; Napoli, C.; Venturi, G.; Pupella, S.; Lombardini, L.; Calistri, P.; Monaco, F.; Cagarelli, R.; Angelini, P.; Bellini, R.; et al. West Nile virus transmission: Results from the integrated surveillance system in Italy, 2008 to 2015. Eurosurveillance 2016, 21.Percivalle, E.; Cassaniti, I.; Sarasini, A.; Rovida, F.; Adzasehoun, K.M.G.; Colombini, I.; Isernia, P.; Cuppari, I.; Baldanti, F. West Nile or Usutu virus? A three-year follow-up of humoral and cellular response in a group of asymptomatic blood donors. Viruses. 2020, 12, E157; DOI:10.3390/v12020157.
  79. Young, J.J.; Coulombier, D.; Domanović, D.; Zeller, H.; Gossner, C.M.; European Union West Nile Fever Working Group. One Health approach for West Nile virus surveillance in the European Union: Relevance of equine data for blood safety. Eurosurveillance 2019, 24, 1800349.Carletti, F.; Colavita, F.; Rovida, F.; Percivalle, E.; Baldanti, F.; Ricci, I.; De Liberato, C.; Rosone, F.; Messina, F.; Lalle, E.; Bordi, L.; Vairo, F., Capobianchi, M.R.; Ippolito, G.; Cappiello, G.; Spanò ,A.; Meschi, S.; Castilletti, C. Expanding Usutu virus circulation in Italy: detection in the Lazio region, central Italy, 2017 to 2018. Euro Surveill. 2019, 24; DOI:10.2807/1560-7917.ES.2019.24.3.1800649.
  80. Vilibic-Cavlek, T.; Vidanović, D.; Barbić, L.; Jeličić, P.; Lazić, S.; Radmanić, L.; Lupulović, D.; Janev-Holcer, N.; Tešović, B.; Milošević, V.; et al. Importance of Multidisciplinary and Regional Collaboration in Integrated West Nile Virus Surveillance—The “One Health” Concept. Infektološki Glasn. 2019, 39, 40–47.Rijks, J.M.; Kik, M.L.; Slaterus, R.; Foppen, R.; Stroo, A.; IJzer, J.; Stahl, J.; Gröne, A.; Koopmans, M.; van der Jeugd, H.P.; Reusken, C. Widespread Usutu virus outbreak in birds in the Netherlands, 2016. Euro Surveill. 2016, 21, 30391; DOI:10.2807/1560-7917.ES.2016.21.45.30391.
  81. Lim, S.M.; Geervliet, M.; Verhagen, J.H.; Müskens, G.J.D.M.; Majoor, F.A.; Osterhaus, A.D.M.E.; Martina, B.E.E. Serologic evidence of West Nile virus and Usutu virus infections in Eurasian coots in the Netherlands. Zoonoses Public Health. 2018, 65, 96-102; DOI:10.1111/zph.12375
  82. Bas Oude Munnink, B.; Münger, E.; Nieuwenhuijse, D.F.; Kohl, R.; van der Linden, A.; Schapendonk, C.M.E.; van der Jeugd, H.; Kik, M.; Rijks, J.M.; Reusken, C.B.E.M.; Koopmans, M. Genomic monitoring to understand the emergence and spread of Usutu virus in the Netherlands, 2016-2018. Sci Rep. 2020, 10, 2798.
  83. Zaaijer, H.L.; Slot, E.; Molier, M.; Reusken, C.B.E.M.; Koppelman, M.H.G.M. Usutu virus infection in Dutch blood donors. Transfusion. 2019, 59(9), 2931-2937; DOI:10.1111/trf.15444.
  84. Hubálek, Z.; Wegner, E.; Halouzka, J.; Tryjanowski, P.; Jerzak, L.; Sikutová, S.; Rudolf, I.; Kruszewicz, A.G.; Jaworski, Z.; Wlodarczyk, R. Serologic survey of potential vertebrate hosts for West Nile virus in Poland. Viral Immunol. 2008, 21, 247-253; DOI:10.1089/vim.2007.0111.
  85. Bażanów, B.; Jansen van Vuren, P.; Szymański, P.; Stygar, D.; Frącka, A.; Twardoń, J.; Kozdrowski, R.; Pawęska, J.T. A survey on West Nile and Usutu viruses in horses and birds in Poland. Viruses. 2018, 10, E87; DOI:10.3390/v10020087.
  86. Lupulović, D.; Martín-Acebes, M.A.; Lazić, S.; Alonso-Padilla, J.; Bláquez, A.B.; Escribano-Romero, E.; Petrovic, T.; Saiz, J.C. First serological evidence of West Nile virus activity in horses in Serbia. Vector Borne Zoonotic Dis. 2011, 11, 1303-1305; DOI:10.1089/vbz.2010.0249.
  87. Escribano-Romero, E.; Lupulović, D.; Merino-Ramos, T.; Blázquez, A.B.; Lazić, G.; Lazić, S.; Saiz, J.C.; Petrović, T. West Nile virus serosurveillance in pigs, wild boars, and roe deer in Serbia. Vet Microbiol. 2015, 176, 365-369; DOI:10.1016/j.vetmic.2015.02.005.
  88. Kemenesi, G.; Buzás, D.; Zana, B.; Kurucz, K.; Krtinic, B.; Kepner, A.; Földes, F.; Jakab, F. First genetic characterization of Usutu virus from Culex pipiens mosquitoes Serbia, 2014. Infect Genet Evol. 2018, 63, 58-61; DOI:10.1016/j.meegid.2018.05.012.
  89. Hrnjaković Cvjetković, I.; Petrovic, T.; Petric, D.; Milosevic, U.; Radovanov, J.; Kovacevic, G.; Jovanović Galović, A.; Patić, A.; Nikolić, N.; Cvjetković, D.; Stefan Mikić, S., Milošević, V. Usutu virus: an emerging flavivirus in Europe. Arch Vet Med. 2017, 10, 25-35.
  90. Petrović, T.; Šekler, M.; Petrić, D.; Vidanović, D.; Potkonjak, A.; Hrnjaković Cvjetković, I.; Savić, S.; Debeljak, Z.; Lazić, G.; Ignjatović Ćupina, A.; Lupulović, D.; Samojlović, M.; Jurišić, A.; Petrović, A.; Ivanović, I.; Milošević, V.; Lazić, S.. Flaviviruses at the territory of Serbia - present situation and challenges. Arch Vet Med. 2018, 11, 53-70.
  91. Petrović, T.; Blázquez, A.B.; Lupulović, D.; Lazić, G.; Escribano-Romero, E.; Fabijan, D.; Kapetanov, M.; Lazić, S.; Saiz, J.C. Monitoring West Nile virus (WNV) infection in wild birds in Serbia during 2012: first isolation and characterisation of WNV strains from Serbia. Euro Surveill. 2013, 18(44), pii=20622.
  92. Čabanová, V.; Šikutová, S.; Straková, P.; Šebesta, O.; Vichová, B.; Zubríková, D.; Miterpáková, M.; Mendel, J.; Hurníková, Z.; Hubálek, Z.; Rudolf, I. Co-circulation of West Nile and Usutu flaviviruses in mosquitoes in Slovakia, 2018. Viruses. 2019, 11, E639; DOI:10.3390/v11070639.
  93. Busquets, N.; Alba, A.; Allepuz, A.; Aranda, C.; Núñez, J.I. Usutu virus sequences in Culex pipiens (Diptera: Culicidae), Spain. Emerg. Infect. Dis. 2008, 14, 861-862; DOI:10.3201/eid1405.07157.
  94. Vázquez, A.; Ruiz, S.; Herrero, L.; Moreno, J.; Molero, F.; Magallanes, A.; Sánchez-Seco M.P.; Figuerola, J.; Tenorio, A. West Nile and Usutu viruses in mosquitoes in Spain, 2008-2009. Am J Trop Med Hyg. 2011, 85, 178-181; DOI:10.4269/ajtmh.2011.11-0042.
  95. Jurado-Tarifa, E.; Napp, S.; Lecollinet, S.; Arenas, A.; Beck, C.; Cerdà-Cuéllar, M.; Fernández-Morente, M.; García-Bocanegra, I. Monitoring of West Nile virus, Usutu virus and Meaban virus in waterfowl used as decoys and wild raptors in southern Spain. Comp Immunol Microbiol Infect Dis. 2016, 49, 58-64; DOI:10.1016/j.cimid.2016.10.001.
  96. Llorente, F.; Pérez-Ramírez, E.; Fernández-Pinero, J.; Soriguer, R.; Figuerola, J.; Jiménez-Clavero, M.A. Flaviviruses in game birds, southern Spain, 2011-2012. Emerg Infect Dis. 2013, 19, 1023-1025; DOI:10.3201/eid1906.130122.
  97. Vanhomwegen, J.; Beck, C.; Desprès, P.; Figuerola, A.; García, R.; Lecollinet, S.; López-Roig, M.; Manuguerra, J.C.; Serra-Cobo, J. Circulation of zoonotic arboviruses in equine populations of Mallorca Island (Spain). Vector Borne Zoonotic Dis. 2017, 17, 340-346; DOI:10.1089/vbz.2016.2042.
  98. Höfle, U.; Gamino, V.; de Mera, I.G.; Mangold, A.J.; Ortíz, J.A.; de la Fuente, J. Usutu virus in migratory song thrushes, Spain. Emerg Infect Dis. 2013, 19, 1173-1175; DOI:10.3201/eid1907.130199.
  99. Ferraguti, M.; LA Puente, J.M.; Soriguer, R.; Llorente, F.; Jiménez-Clavero, M.Á.; Figuerola, J. West Nile virus-neutralizing antibodies in wild birds from southern Spain. Epidemiol Infect. 2016, 144, 1907-1911; DOI:10.1017/S0950268816000133.
  100. García-Bocanegra, I.; Paniagua, J.; Gutiérrez-Guzmán, A.V.; Lecollinet, S.; Boadella, M.; Arenas-Montes, A.; Cano-Terriza, D.; Lowenski, S.; Gortázar, C.; Höfle, U. Spatio-temporal trends and risk factors affecting West Nile virus and related flavivirus exposure in Spanish wild ruminants. BMC Vet Res. 2016, 12, 249.
  101. Cano-Terriza, D.; Guerra, R.; Lecollinet, S.; Cerdà-Cuéllar, M.; Cabezón, O.; Almería, S.; García-Bocanegra, I. Epidemiological survey of zoonotic pathogens in feral pigeons (Columba livia var. domestica) and sympatric zoo species in Southern Spain. Comp Immunol Microbiol Infect Dis. 2015, 43, 22-27; DOI:10.1016/j.cimid.2015.10.003.
  102. Steinmetz, H.W.; Bakonyi, T.; Weissenbock, H.; Hatt, J.M.; Eulenberger, U.; Robert, N.; Hoop, R.; Nowotny, N. Emergence and establishment of Usutu virus infection in wild and captive avian species in and around Zurich, Switzerland–genomic and pathologic comparison to other central European outbreaks. Vet. Microbiol. 2011, 148, 207-212; DOI:10.1016/j.vetmic.2010.09.018.
  103. Cordey, S.; Vieille, G.; Turin, L.; Kaiser, L. Usutu virus in cerebrospinal fluid: A 2-year survey in a tertiary care hospital, Geneva, Switzerland. J Med Virol. 2018, 90, 609-611; DOI:10.1002/jmv.24967.
  104. Engler, O.; Savini, G.; Papa, A.; Figuerola, J.; Groschup, M.H.; Kampen, H.; Medlock, J.; Vaux, A.; Wilson, A.J.; Werner, D.; Jöst, H.; Goffredo, M.; Capelli, G.; Federici, V.; Tonolla, M.; Patocchi, N.; Flacio, E.; Portmann, J.; Rossi-Pedruzzi, A.; Mourelatos, S.; Ruiz, S.; Vasquez, A.; Calzolari, M.; Bonilauri, P; Dottori, M.; Schaffner, F.; Mathis, A.; Johnson, N. European surveillance for West Nile virus in mosquito populations. Int J Environ Res Public Health. 2013, 10, 4869-4895; DOI: 10.3390/ijerph10104869
  105. Wipf, N.C.; Guidi, V.; Tonolla, M.; Ruinelli, M.; Müller, P.; Engler, O. Evaluation of honey-baited FTA cards in combination with different mosquito traps in an area of low arbovirus prevalence. Parasit Vectors. 2019, 12, 554; DOI:10.1186/s13071-019-3798-8.
  106. Buckley, A.; Dawson, A.; Moss, S.R.; Hinsley, S.A.; Bellamy, P.E.; Gould, E.A. Serological evidence of West Nile virus, Usutu virus and Sindbis virus infection of birds in the UK. J Gen Virol. 2003, 84, 2807-2817; DOI: 10.1099/vir.0.19341-0.
  107. Buckley, A.; Dawson, A.; Gould, E.A. Detection of seroconversion to West Nile virus, Usutu virus and Sindbis virus in UK sentinel chickens. Virol. J. 2006, 3, 71; DOI:10.1186/1743-422X-3-71.
  108. Horton, D.L.; Lawson, B.; Egbetade, A.; Jeffries, C.; Johnson, N.; Cunningham, A.A.; Fooks, A.R. Targeted surveillance for Usutu virus in British birds (2005-2011). Vet Rec. 2013, 172, 17; DOI:10.1136/vr.101275.
  109. Vaux, A.G.; Gibson, G.; Hernandez-Triana, L.M.; Cheke, R.A.; McCracken, F.; Jeffries, C.L.; Horton, D.L.; Springate, S.; Johnson, N.; Fooks, A.R.; Leach, S.; Medlock, J.M. Enhanced West Nile virus surveillance in the North Kent marshes, UK. Parasit Vectors. 2015, 8, 91; DOI:10.1186/s13071-015-0705-9.
  110. Mannasse, B.; Mendelson, E.; Orshan, L.; Mor, O.; Shalom, U.; Yeger, T.; Lustig, Y. Usutu virus RNA in mosquitoes, Israel, 2014-2015. Emerg Infect Dis. 2017, 23, 1699-1702; DOI: 10.3201/eid2310.171017.
  111. Clé, M.; Beck, C.; Salinas, S.; Lecollinet, S.; Gutierrez, S.; Van de Perre, P.; Baldet, T.; Foulongne, V.; Simonin, Y. Usutu virus: A new threat? Epidemiol Infect. 2019, 147, 232; DOI:10.1017/S0950268819001213.
  112. Rizzo, C.; Napoli, C.; Venturi, G.; Pupella, S.; Lombardini, L.; Calistri, P.; Monaco, F.; Cagarelli, R.; Angelini, P.; Bellini, R.; Tamba, M.; Piatti, A.; Russo, F.; Palù, G.; Chiari, M.; Lavazza, A.; Bella, A.; the Italian WNV surveillance working group. West Nile virus transmission: results from the integrated surveillance system in Italy, 2008 to 2015. Euro Surveill. 2016, 21(37), pii=30340. DOI: 10.2807/1560-7917.ES.2016.21.37.30340.
  113. Young, J.J.; Coulombier, D.; Domanović, D.; European Union West Nile fever working group, Zeller, H.; Gossner C.M.; European Union West Nile fever working group. One Health approach for West Nile virus surveillance in the European Union: relevance of equine data for blood safety. Euro Surveill. 2019, 24(16), pii=1800349. DOI: 10.2807/1560-7917.ES.2019.24.16.1800349.
  114. Vilibić-Čavlek, T.; Petrović, T.; Savić, V.; Šekler, M.; Klobučar, A.; Petrić, D.; Tabain, I.; Vidanović, D.; Bogdanić, M.; Lazić, G.; Cvjetković-Hrnjaković, I.; Stevanović, V.; Milošević, V.; Tešović, B.; Janev-Holcer, N.; Lupulović, D.; Radmanić, L.; Lazić, S.; Jeličić, P.; Barbić, Lj. Importance of multidisciplinary and regional collaboration in integrated West Nile virus surveillance - The "One health" concept. Croat J Infect. 2019, 39(2), 40-7; DOI:10.37797/ig.39.2.2.
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