Plectranthus neochilus Schltr. (Lamiaceae) is a plant recently introduced in Cuba. Worldwide, it is an ethnomedicinal alternative for its use against microbial infections, but the Cuban population use the extracts to treat sleep disorders. To address this apparent incongruity, four collections (from different seasonal conditions in the year) of Cuban P. neochilus cultivars were analyzed in terms of their pharmacognostic characteristics.
1. Introduction
The World Health Organization (WHO) highlights that about 80% of the population from developing countries make use of medicinal plant remedies as a cost-effective way to solve health problems. Low-income countries have limited access to expensive commercial drugs on the international market, which forces people to resolve health issues with natural herbal plants/remedies
[1]. In this context, natural products or natural product derivatives continue to lead the process of obtaining new entities in drug discovery. In fact, of the 1881 new drugs approved in the period between 1981 and 2019, 787 (41.8%) are directly related to a natural origin. In addition, 489 analogs were synthesized that mimic natural compounds, which together represent two-thirds of the novel drugs
[2]. The importance of natural products in health management also becomes apparent from the average growth rate of the global trade of medicinal herbal derivatives, reaching an estimated market of USD 83 billion in 2014
[3]. Therefore, natural medicines should be the focus of underdeveloped countries not only as a way to solve their own health problems but also to get access to new income sources.
All those factors increase the common practice to introduce non-native medicinal plants in countries, with the purpose to reproduce this source of raw material either by private/individual or governmental entities for medical/commercial purposes. However, this intention to cultivate non-native medicinal plants will not always lead to the exact reproduction of the bioactive properties that characterize them in their natural habitat
[4]. Intrinsic factors can be better controlled by determining the identity of the plant, as well as its genetic authenticity, but the external factors, such as environment, cultivation, harvest, and post-harvest processing/storage practices, become more difficult to manage, especially when the activity is developed by the population
[5].
Plectranthus neochilus Schltr., an aromatic and succulent species from the Lamiaceae, is one of these plants whose cultivation has spread to almost the entire world. The endemic plant from South Africa and Namibia is commonly known as ‘blue coleus’ or ‘lobster flower’ (English speakers), ‘muskietbossie’ by Africans, and ‘boldo-rasteiro’ by Portuguese. It is traditionally used in African and Brazilian folk medicine to treat skin diseases, respiratory ailments, disturbed digestion, hepatic insufficiency, and dyspepsia
[6][7][6,7]. From the pharmacologic point of view, antioxidant
[8], antibacterial
[9], cytotoxic
[10], and α-glucosidase-inhibitory activities
[11] of
P. neochilus have been reported, but prevailing is the antimicrobial activity. In a recent paper, it was demonstrated that even after being used for phytoremediation, the essential oils from this plant conserves its high antimicrobial profile
[12].
A few years ago,
P. neochilus was introduced in Cuba and quickly started to be cultivated because of its ethnobotanical and pharmacological benefits and its ornamental properties
[13]. Recently, an ethnobotanical survey revealed that this plant is mainly consumed by the Cuban population for its sedative and hypnotic effects rather than for its traditional antimicrobial and antidiabetic properties
[14]. Due to the recent introduction, its wide use, and the lack of information other than ethnobotany on this species in Cuba, the purpose of this research was to investigate the pharmacognostic parameters, the phytochemical profile of leaves and extracts of
P. neochilus cultivated in Cuba, as well as its antimicrobial activity.
2. Plant Quality Control Parameters
2.1. Fresh Leaves
Macroscopic determinations did not reveal phenotypical differences throughout the year of study (four lots collected) and are in agreement with the deposited botanical information for this plant in Cuba
[13] and Brazil
[15]. Leaves with creased edges, perinervic venation, petiole with a wedge-shaped base, membranous texture, greenish-gray color, and a strong and characteristic aromatic odor are the most representative macroscopic quality control parameters. Other remarkable characteristics are an adaxial and abaxial pubescent leaf surface, with generally short hairs. In spite of the strong and characteristic aromatic odor, none of the eight determined essential oil (EO) yields (two replicates for the four lots) allows us to obtain quantifiable values greater than the sensitivity of the determination method. Accordingly, the EO yield values (EOY) measured did not exceed the detection limit (EOY < 0.01%). In general, the EO is detected at relatively low yields. For the species that grows in Brazil, values around 0.03% have been regularly reported
[6][16][6,16], while for the species that grow in Portugal
[8] and South Africa, yields exceed 0.2%
[17].
The micro-morphological analysis revealed that both leaf surfaces are covered with trichomes and abundant orange-colored glandular cells that are more evident in the adaxial surface when observed under the stereo-microscope 40× (
Figure 1A). The multicellular and uni-serial nature of non-glandular trichomes (
Figure 1B), as well as the orange-colored glandular cells (
Figure 1C), can be better observed when using higher magnifications of the bright field microscope at 100×. The adaxial surface has a cuticle of approximately 1 μm thick with a single layer epidermis with polygonal cells of 10 μm in size, periclinal and anticline walls, tracing straight or convex lines that are up to 1.25 µm thick (
Figure 1D). The abaxial epidermis is also 1.25 µm thick without a cuticle and has diacytic stomata (
Figure 1E) with a density of 22.5/100 µm
2. The parenchyma is homogeneous, lacunar, with five to six strata of amorphous cells reaching a thickness of 35 µm. Most of these observations match with the micro-morphological characteristics described for the species, which were recently updated, with the only exception of the presence of glandular trichomes
[18].
Figure 1. Photomicrograph of transverse sections of leaves from Plectranthus neochilus collected in Santiago de Cuba. (A) Adaxial surface of the foliar lamina. (B) Non-glandular trichomes. (C) Orange-colored glandular cells. (D) Polygonal cells. (E) Diacytic stomata. (A) × 40× and (B–E) × 100×.
2.2. Dry Leaves
The drying process was carried out by sun-drying, shade-drying, and oven-drying (45 °C). Due to the succulent characteristics, none of the air-exposure methods was effective since more than three weeks were needed to achieve drying, causing microbiological contamination in the case of shade-dried batches. Under these circumstances, only the oven-dried method was able to eliminate the water from the leaves in 6–8 days, preserving most of the organoleptic characteristics. The residual moisture content was 10.5 ± 1.7% (azeotropic method) (n = 8, two determinations on the four batches).
The other quality control parameters repeatable within batches (
Table 1) showed no appreciable differences between the different harvesting moments. In all cases, it was possible to define an upper and lower decision limit, allowing to predict a range in which the results should be expected. Even when no statistical differences (
p > 0.05) among batches were found for none of the quality control parameters, the batch selected to prepare the extracts from
P. neochilus leaves was batch 2 (May), considering the maximal number of soluble compounds.
Table 1. Quantitative quality control parameters of dried leaves from Plectranthus neochilus collected in Santiago de Cuba at different times.
Parameter |
Batch 1
(February) |
Batch 2
(May) |
Batch 3
(August) |
Batch 4
(November) |
LDL (95%) |
UDL (95%) |
Total ash content (%) |
8.1 | a | ± 1.4 |
8.4 | a | ± 2.0 |
9.7 | a | ± 1.3 |
8.5 | a | ± 1.7 |
4.8 |
12.5 |
Ethanol total soluble substances (%) |
18.5 | b | ± 2.0 |
20.2 | b | ± 2.7 |
17.5 | b | ± 1.9 |
20.2 | b | ± 0.4 |
14.5 |
23.7 |
Water total soluble substances (%) |
22.6 | c | ± 1.3 |
24.1 | c | ± 1.4 |
22.1 | c | ± 1.1 |
23.5 | c | ± 1.6 |
19.8 |
26.3 |