SFKs in intestine: Comparison
Please note this is a comparison between Version 1 by Audrey Sirvent and Version 4 by serge roche.

Src, originally identified as an oncogene, is a membrane-anchored tyrosine kinase (TK) and the Src family kinase (SFK) prototype. SFKs regulate the signalling induced by a wide range of cell surface receptors leading to epithelial cell growth and adhesion. In the intestine, the SFK members Src, Fyn and Yes regulate epithelial cell proliferation and migration during tissue regeneration and transformation, thus implicating conserved and specific functions. In patients with colorectal cancer (CRC), SFK activity is a marker of poor clinical prognosis and a potent driver of metastasis formation. These tumorigenic activities are linked to SFK capacity to promote the dissemination and tumour-initiating capacities of epithelial tumour cells. However, it is unclear how SFKs promote colon tumour formation and metastatic progression because SFK-encoding genes are unfrequently mutated in human cancer. Here, we review recent findings on SFK signalling during intestinal homeostasis, regeneration and tumorigenesis, and discuss about therapeutic strategies to efficiently target Src signalling in CRC

  • Src family kinases
  • intestinal homeostasis
  • cell signalling
  • colon cancer
  • cancer therapy

1. Introduction of SFKs

  1. Introduction of SFKs

 Src, originally identified as an oncogene, is a membrane-anchored cytoplasmic TK that mediates signalling induced by a wide range of cell surface receptors [1][2]. Notably, Src is a master regulator of cell growth and migration induced by extracellular cues. Src is also the prototype of SFK that includes eight members (Src, Fyn, Yes, Lck, Fgr, Hck, Blk and Lyn) of which three (Src, Fyn and Yes) are widely expressed [3][2]. Src shares with the other SFKs a common modular structure formed by the membrane-anchoring SH4 region through lipid attachment (i.e., myristoyl), followed by an intrinsically disordered region named the unique domain (UD), and the SH3, SH2 and kinase domain [3][2]. SH4 contains also a palmitoylation site for membrane anchoring, except in Src and Blk. The kinase domain is bordered by two short regulatory sequences, named the linker and the tail, involved in the tight regulation of the kinase activity to prevent aberrant protein tyrosine phosphorylation. Crystallography studies revealed that intramolecular interactions are part of the mechanisms that control SFK catalytic activity [4]. Notably, phosphorylation of a conserved tyrosine in the tail (Tyr 530 in the human Src sequence) promotes SH2 intramolecular interactions that, combined with the SH3-linker interaction, stabilise the enzyme in an autoinhibited conformation. This Src phospho-regulatory mechanism is conserved in all SFKs and is mediated by the cytoplasmic TKs C-terminal Src Kinase (CSK) and CSK homologous kinase [5]. Disruption of any of the SH2 or SH3-mediated protein interactions or tyrosine phosphatase activity leads to the kinase active conformation. Catalytic de-repression enables SFK autophosphorylation of the activation loop (Tyr419 in the human Src sequence) that further supports the kinase active state [4]. In agreement with this model, stabilization of the enzyme in a de-repressed conformation by somatic mutation or protein association results in constitutive SFK activity that can lead to oncogenic properties [6].

Recent findings revealed additional unsuspected Src regulatory mechanisms involving its UD. This unstructured region of about 70–80 amino acids is conserved in vertebrates and is unique among the different SFKs (e.g., different sequences). Although Src UD function remained mysterious until recently, NMR analyses revealed that this region has a compact, yet highly dynamic structure, described as an intramolecular fuzzy complex [7]. NMR-guided mutations that affect UD-SH3 interactions revealed an essential role for this fuzzy complex in Src signalling leading to CRC cell migration [8]. Moreover, Src can dimerise through involvement of the UD in the binding to a hydrophobic pocket within the kinase domain of the dimeric partner [9]. A biophysical study showed that the Src SH4 domain has dimerisation capacity on its own, suggesting a complex mechanism underlying Src dimerisation [10]. Importantly, Src dimerisation may define a novel regulatory mechanism because it substantially enhances Src autophosphorylation and phosphorylation of selected substrates [9]. Interestingly, SFKs share well-conserved sequence features involving aromatic residues in their UDs, suggesting a similar UD-dependent regulatory region in the other SFKs [7].

Finally, emerging evidence supports the existence of an additional regulatory mechanism through Src myristoylation, as described for the cytoplasmic TK Abelson (ABL) where a myristoyl binding pocket in the kinase domain maintains ABL in an inactive state [11]. Structural analyses suggested, but did not confirm yet, the presence of a similar binding pocket in Src [12]. Nevertheless, Moasser et al. reported that Src dimerisation also involves the interaction of the myristoylated N-terminal region with the kinase domain pocket in trans [9]. Surprisingly, Pons et al. discovered an additional myristoyl binding site in Src-SH3, that contributes to Src membrane anchoring [13]. Interestingly this interaction is modulated by the fuzzy complex contained in the UD, suggesting a mechanism linking Src activation and membrane anchoring. Therefore, we predict an important role for the SH4 and the UD in controlling the Src topology at the membrane or the local microenvironment for substrate selection and signalling. Whether this myristoylation-switch mechanism is conserved in other SFKs is currently unknown. Overall, these recent findings uncover a much higher complexity of SFK regulation than previously expected, which may have important implications on the SFK’s oncogenic functions.

2. SFKs in Intestinal Homeostasis and Regeneration

  1. SFKs in Intestinal Homeostasis and Regeneration

 Genetic analyses in animals established essential physiological roles for SFKs [14]. For instance, constitutive SFK gene knock-out experiments in mice revealed an important function for Src in early development, at least partially shared with Fyn and Yes. Specifically, Src-deficient mice die early after birth because of defects in bones where Src is normally highly expressed. Conversely, combined Src, Fyn and Yes gene inactivation leads to mouse embryonic lethality [14]. Consistent with this, disruption of the SFK negative regulator Csk leads to embryonic lethality with excessive tissue SFK activity, indicating that SFK regulation is essential for mouse development [14]. However, other genetic analyses revealed that Src, but not Fyn, is partly epistatic to the Csk gene, consistent with SFK partially redundant functions during development [13]. Then, tissue-specific gene manipulation studies showed important roles for SFKs in epithelial tissues. For instance, Cordero et al. performed SFK gain and loss of function experiments in mouse and fruit fly intestines to address SFK’s physiological role in the intestinal epithelium [15]. Drosophila is a useful genetic model to study intestinal homeostasis because, as in mammals, adult fly midgut epithelium is renewed by intestinal stem cells (ISC) [16]. Src42A and Src64B are the two Src-like kinases expressed in Drosophila, and are the likely orthologues of Src and Fyn, respectively. However, only Src42A loss of function inhibits ISC proliferation in conditions of homeostasis and stress response to bacterial infection, suggesting a specific Src function in the intestine. Nevertheless, genetic overactivation of any of these SFKs is sufficient to drive ISC hyperproliferation, indicating potential SFK redundant functions above a certain threshold [15][17]. An Src key role was confirmed in a mouse model where Src ablation in the intestine prevented ISC proliferation and crypt regeneration after induction of DNA damage by gamma irradiation [15]. However, Src ablation alone was not sufficient to affect intestinal homeostasis because of its overlapping functions with Fyn and Yes. In agreement, combined inactivation of Src, Fyn and Yes in the intestine leads to intestinal epithelial cell (IEC) apoptosis and reduction of the number of Paneth cells in the small intestine [15], Conversely, ablation of their negative regulator Csk increases IEC proliferative activity and turnover [18] (Figure 1).

Mechanistically, Src drives ISC proliferation through upregulation of EGFR, activation of Ras/MAPK and signal transducer and activator of transcription 3 (Stat3) signalling [15]. This Src function was revealed after intestinal injury induced by irradiation. The mechanism underlying Src-mediated ISC proliferation is not fully clear, but may implicate important intestinal regulators, such as Wnt/beta-catenin signalling [15], that controls proliferation and differentiation of crypt-localised ISCs, and Notch that controls the enterocyte lineage [19] (Figure 1B). Src also uses the transcription factor Yes Associated Protein (YAP), an essential sensor of the cell microenvironment structural and mechanical features [20], to mediate epithelial regeneration during intestinal inflammation. This new Src signalling activity was uncovered from a genetic mouse model with persistent intestinal inflammation upon IEC overexpression of gp130, the coreceptor for interleukins of the IL-6 family [21]. These animals display aberrant IEC proliferation and differentiation and are resistant to mucosal erosion. This gp130 activity is mediated by interaction with Src and Yes to phosphorylate the transcription factor YAP on specific tyrosine residues and to induce its stabilisation and nuclear translocation [22]. Surprisingly, this inflammatory mechanism is independent from the effector Stat3 [21]. A similar Src/YAP signalling has been described during intestinal regeneration mediated by dietary and metabolic factors [23] strengthening the conserved role of Src/YAP signalling in intestinal repair. Src may also induce ISC proliferation through a cell nonautonomous mechanism. Indeed, in Drosophila gut, upon bacterial infection, Src activation in enterocytes induces IL-6 expression that leads to ISC proliferation [24]. Similarly, in the mouse, in physiological conditions, intestinal tissue is renewed by Leucine-rich repeat-containing G-protein coupled receptor 5-positive (Lgr5+) ISCs localised at the bottom of the crypt [25]. However, upon severe damage, intestinal epithelium can be regenerated by a distinct mechanism [26] that involves IEC dedifferentiation via transient foetal-like features [27]. Importantly, this regenerative mechanism is mediated by extracellular matrix remodelling that enables biomechanic Src/YAP signalling for efficient tissue repair [27] (Figure 1B). A similar mechanism was reported for Class A basic helix-loop-helix protein 15-positive (Bhlha15+) intestinal secretory precursors that transiently convert into enterocyte progenitors after doxorubicin-induced epithelial injury [19]. In the mouse, elevated SFK activity induced by Csk ablation in IECs activates an additional Rac signalling mechanism to promote IEC proliferation [18]. Interestingly, these animals display enhanced susceptibility to colitis induced by the chemical irritant dextran sodium sulphate (DSS), due to low epithelial barrier function caused by tight junctions reduction [28].

Finally, SFKs have also specific functions in immune cells. Indeed, in Fyn-deficient mice exposed to DSS to induce intestinal injury, the number of CD4+FOXP3+ cells was reduced as well as the capacity of lymphocytes to differentiate into regulatory T cells [29]. This indicates that Fyn has a protective role against intestinal injury. Using a similar approach it was demonstrated that Lyn also is protective against intestinal injury, microbiota-dependent intestinal inflammation and susceptibility to enteric pathogens [30]. Another mouse strain with an activated Lyn mutation (LynY508F) revealed a Lyn key role in the control of the innate immune response and validated its protective role against colitis [31].

Figure 1. SFKs in intestinal homeostasis, regeneration and transformation. (A) SFKs regulate ISC proliferation, Paneth cell differentiation and IEC survival during intestinal homeostasis. SFKs, Wnt and Slap activity in the intestinal epithelium is indicated. (B) SFKs mediate intestinal regeneration by activating ISC proliferation. (C) SFKs mediate tumour formation induced by activating CSC survival. Src signalling involved in intestinal regeneration and transformation is indicated.

 

3. SFKs in Intestinal Cell Transformation

  1. SFKs in Intestinal Cell Transformation

 Intestinal transformation is mainly caused by deregulated ISC proliferation [32], that can be mediated by SFKs (Figure 1C) [21]. Their transforming activity was revealed using genetically-modified animal models [21]. In CRC, the most frequent tumour-initiating event is abnormally elevated Wnt/beta-catenin signalling. Mechanistically, Apc inactivation leads to protein stabilization and activation of beta-catenin transcriptional activity [33]. Additionally, Apc inactivation leads to upregulation of SFK activity in the hyperproliferative “crypt progenitor cell-like” domain of the intestinal epithelium [21][32]. Src gene inactivation in IECs revealed its essential role in intestinal tumorigenesis-induced by Apc loss in mouse and fly models [21]. Specifically, conditional Src inactivation in IECs impairs adenoma tumour initiation and progression. However, this Src activity is not mediated by MAPK or STAT3 signalling, contrary to what is observed during intestinal regeneration [21]. In agreement, Apc inactivation also induces local inflammation due to a reduction in intestinal epithelial barrier function, which also contributes to tumour development [34]. Mechanistically, colon microbiota invasion activates IL-23-producing myeloid cells and expand tumour-resident IL-17-producing T lymphocytes, resulting in proliferation of transformed IECs through IL-11 receptor and gp130 upregulation, and eventually gp130/Src/YAP signalling [35][36]. Importantly, a positive autoregulatory loop controls gp130 and YAP expression, enabling tumour development. In agreement, in transgenic mice that express an active gp130 mutant in IECs, intestinal tumour development is accelerated upon Apc inactivation. Importantly, this gp130 pro-tumoral function depends on SFK activity [36]. The intestinal tumour-promoting role of other SFKs is largely unknown. Nevertheless, a recent study uncovered an important HCK function in colon tumorigenesis [37]. They observed that mutant mice carrying an oncogenic mutation in the Hck gene (HCKY520F) are prone to colon cancer development when treated with DSS and the carcinogen azoxymethane (AOM). This tumour-promoting effect was associated with HCK capacity to induce tumour-promoting M2-like macrophages and the accumulation of IL-6/lL-11 family cytokines [37].

4. Therapeutic Strategies to Target SFKs Signalling in CRC

  1. Therapeutic Strategies to Target SFKs Signalling in CRC

 4.1. Therapeutic Utility

Accumulated evidences obtained in experimental CRC models suggest that SFKs could be attractive targets in advanced CRC. SFK inhibition may be of clinical value in advanced CRC due to their role in CRC cell dissemination and CSC activity, which are the main causes of tumour relapse and metastatic progression. In agreement, specific Src inhibitors (Srci) reduce liver metastasis development in nude mouse models, an effect associated with decreased tumour angiogenesis, cell proliferation and survival [38][39]. However, it was not assessed whether SFK inhibition reduces CSC activity. In addition, Src oncogenic role in RTK signalling may explain why Srci sensitises CRC to RTK inhibitors in experimental CRC models [40]. Similarly, the effect of Src activity on MAPK/PI3K signalling is consistent with findings showing the potential clinical utility of combining Srci with KRAS effector inhibitors (MAPK kinase, and PI3K inhibitors) in KRAS mutant CRC [41][42]. This observation is clinically relevant because these tumours are refractory to the upstream EGFR antibody currently used in the clinic, cetuximab [43]. Src activity has been identified as a mechanism of tumour resistance to oxaliplatin in metastatic CRC, suggesting that its pharmacological inhibition could enhance the efficacy of oxaliplatin-based chemotherapy in patients with CRC [44][45]. Finally, some evidences suggest that Srci might sensitise CRC to anti PD-L1 immune checkpoint inhibitors [46][47].

4.2. Therapeutic Strategies

Several Src-like ATP competitive inhibitors have been developed for oncology, including dasatinib, bosutinib and saracatinib [48]. Although dasatinib and bosutinib were originally developed to target Src/Abl activities, they are multikinase inhibitors [49][50][51]. Moreover, tirbanibulin, a Src-like peptide binding site inhibitor, inhibits also tubulin polymerization [52]. Although most of these Srci display significant anti-tumour activity in experiment tumour models, they gave disappointing results in patients with CRC, both as monotherapy and in combination with the current therapies [53][54][55]. For instance, the combination of dasatinib with the chemotherapy regimen FOLFOX (folinic acid, 5-fluorouracil, and oxaliplatin) with or without cetuximab did not show any meaningful clinical activity in refractory CRC [55].

The complex mechanisms of Src regulation and hyperactivation in CRC discussed above may explain the lack of anticancer effect of these drugs, particularly the lack of patient stratification, drug efficacy and selectivity, resulting in significant toxicity. These complex mechanisms also suggest new therapeutic strategies to better target Src signalling in CRC (Figure 2).

Figure 2. Therapeutic strategies to efficiently target Src signalling in CRC, including drug inhibition of Src UD signalling, kinase dimerisation, membrane localisation, drugs that reactivate Src inhibitors and drugs that inhibit activity of downstream signals.

For instance, several noncatalytic strategies could be developed to improve Src inhibition in CRC, including allosteric inhibitors of the myristoyl switch regulatory mechanism, as recently demonstrated with asciminib in BCR-ABL expressing chronic myeloid leukaemia [56]. Small molecules that interfere with Src UD signalling, kinase dimerisation or membrane localisation by disrupting UNC119-Src interaction may lead to Srci with higher specificity and lower toxicity [57]. Additionally, drugs that reactivate SRC inhibitors, such as PAG or SLAP, could limit CRC invasion or metastatic reactivation. Finally, patient selection based on Src activity level in CRC would clearly improve the overall therapeutic response. While CMS2 tumours should preferentially respond to Srci, studies in advanced CRC suggest that left-sided tumours with elevated RTK signalling are also good targets [58][59]. Moreover, there is no validated biomarker for Src-dependent tumours. Nevertheless, several candidates could be proposed, such as high Src tumour activity or SRC copy number, high phosphorylation of Src effectors, such as FAK, YAP/TAZ, RTKs, or even high tyrosine phosphorylation level. However, high SLAP expression could limit Src oncogenic signalling, and therefore, tumours could be less responsive to Srci, despite their high aberrant Src activity [60]. Therefore, SLAP expression could be an additional predictor of the tumour response to Srci.

5. Conclusions and Perspectives

  1. Conclusions and Perspectives

 Since the first observation of abnormal Src activity in CRC samples [61], much has been learned about SFK physiological and oncogenic functions in the intestine. Although, their oncogenic roles have been underestimated because of the absence of frequent somatic mutations in CRC, there is now strong evidence of their detrimental role in CRC cell invasion. This suggests that Srci could be useful for the management of patients with metastatic CRC. However, Srci clinical utility in CRC has not been demonstrated yet, because of lack of patient stratification, drug efficacy and selectivity. Clearly much more needs to be learned about how SFKs function during CRC development to reach this objective. Recent molecular studies have highlighted the much higher complexity of SFK regulation, which needs to be investigated in order to efficiently target these activities. Future studies on SFK physiological roles in the intestine may bring important insights into SFK influence on CRC development. Moreover, appropriate CRC models are crucially needed, including genetically modified mice, to recapitulate some of the activating mechanisms reported in human CRC, in order to assess the complexity of Src signalling. It would be important to analyse the respective oncogenic roles of these SFKs in the epithelial and microenvironment compartments of these tumours. Moreover, their contribution to metastatic reactivation and immune evasion also are important questions that could be addressed with these models. Finally, phosphoproteomic studies are needed to decipher the molecular complexity of Src signalling in CRC. For instance, the large group of mRNA regulators identified in such studies [62] points to an unsuspected feature of Src tumour activity. Overall, future studies should allow understanding of how SFKs regulate epithelial homeostasis and tumorigenesis, and improving Src-based therapies in CRC.

 

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