Medicinal plants have been used since the beginning of human civilization to treat various diseases. Different properties have been discovered for each plant, due to many researchers’ focus on plants as a natural resource for treating human health ^[1][2][1,2]. Of the various medicinal plants, species belonging to the Prosopis genus have been widely used in folk medicine. The Prosopis genus belongs to the Fabaceae or Leguminosae family, and includes about 45 species of spiny trees and shrubs. This genus is found in both subtropical and tropical areas of the world. Briefly, species belonging to the Prosopis genus have been traditionally used for the treatment of asthma, birth/postpartum pains, callouses, conjunctivitis, diabetes, diarrhea, expectorant, fever, flu, lactation, liver infection, malaria, otitis, pains, pediculosis, rheumatism, scabies, skin inflammations, spasm, stomach ache, removal of bladder and pancreas stones, among other applications ^[3][4][5][3,4,5]. On the other hand, in addition to being being used for centuries for medicinal purposes Prosopis plants are also of commercial interest. The paste, gum, and leaves and pods smoke of Prosopis plants possess various bioactive properties, such as anticancer, antidiabetic, anti-inflammatory, antimicrobial and antioxidant effects ^[6][7][8][6,7,8]. These effects have been increasingly correlated with their content in phytoconstituents, namely flavonoids, tannins, alkaloids, quinones and phenolics. Indeed, Prosopis plants have been reported as a rich source of phenolic compounds, being anthocyanins and the flavonoids apigenin, luteolin, quercetin and their derivatives the most abundant ones ^[9][10][11][9,10,11].

Thus, and given the above highlighted aspects, the aim of this review is to provide an in-depth overview of the literature data on the biological activities of the Prosopis genus, and to present its potential benefits and applications in both the pharmaceutical and food industries.

2. Prosopis Plants Phytochemical Composition

Epidemiological studies have suggested an inverse association between the consumption of phytochemicals (such as carotenoids and phenolics) and a reduced risk of certain diseases, namely chronic disorders [12]. Medicinal plants, and specifically Prosopis plants, are rich sources of phytochemicals, among them alkaloids, phenolic compounds, particularly flavonoids (Figure 1; Figure 2) and phenolic acids (Figure 3), glycosides, steroids, tannins and triterpenoids, increasingly recognized as having positive health effects.

Figure 1. Structures of the main flavonoids in Prosopis plants.

Figure 2. Structures of some C- glycosyl flavones from Prosopis plants.

Figure 3. Chemical structures of some phenolic acids from Prosopis plants.

Several studies have been conducted to identify and quantify the chemical composition of Prosopis plants. The most commonly studied plants and their abundance in phytochemicals are listed in Table 1.

Table 1. Identification/quantification of phytochemicals in Prosopis species.

Prosopis Plant and Part	Identified/Quantified Phytochemicals	References
P. alba flours	Isovitexin (1.12–0.48 μg/mg)	[9]
	Vicenin II (1.07–0.34 μg/mg)
	Vitexin (0.91–0.47 μg/mg)
	Schaftoside (0.42–0.00 μg/mg)
	Ferulic acid (4.01–0.28 μg/mg)
	Coumaric acid (3.94–0.33 μg/mg)
P. alba pods	Q-dihexoside rhamnoside	[10]
	Q-dihexoside
	Q-methylether dihexoside
	Vitexin
	Q-rhamnoside hexoside
	Isovitexin
	Q-hexoside
	K-hexoside
P. alba flour	Isoschaftoside hexoside (2.43 mg/g)	[13]
	Schaftoside hexoside (3.33 mg/g)
	Vicenin II/Isomer (0.67 mg/g)
	Vicenin II/Isomer (2.34 mg/g)
	Gallic acid (8.203 mg/g)
	[
			21
]
		Hydroxybenzoic acid (1.797 mg/g)
Pyrocatechol (5.538 mg/g)
Caffeic acid (0.295 mg/g)
Ferulic acid (0.466 mg/g)
Quercetin (0.045 mg/g)

3. Traditional Medicinal Uses of Prosopis Plants

Recent ethnopharmacological studies have shown that P. africana, P. alba, P. cineraria, P. farcta, P. glandulosa, P. juliflora, P. nigra, P. ruscifolia and P. spicigera are amongst the most commonly used Prosopis plants in folk medicine treatments. As shown in Table 2, different Prosopis plants parts are used in diverse regions of the world for the treatment of various diseases. In particular, the positive health effects of P. cineraria, P. juliflora and P. africana have been well-documented ^{[3][4][5][13][14][15][16][17][18][19][20][21][22]}[3,4,5,13,14,15,16,17,18,19,20,21,22].

Table 2. Prosopis plants traditionally used in the treatment of various diseases in diverse regions of the world.

Scientific Name	Location	Local Name	Parts Used	Administration	Disease(s) Treated/Bioactive Effects	References
P. africana	Sélingué subdistrict, Mali	Guele	Bark trunk	Oral, Bath	Malaria	[19]
	Guinea-Bissau	Tentera, Buiengué, Bussagan, Coquengue karbon, Késeg-késeg, Paucarvão, Pócarvão, Pó-de-carbom, Po-di-carvom, Tchelem, Tchalem-ai, tchela, Tchelangadje, Tchelem, Bal-tencali, Culengô, Culim-ô, Djandjam-ô, Quéssem-quéssem, Djeiha, Ogea	Leaves, bark, roots	Unspecified	Pains, pregnancy (childbirth, breastfeeding, diseases of the newborn), skin inflammations (wounds, burns)	[20]
	Nsukka Local Government Area, South-eastern Nigeria	Ugba	Leaf	Oral	Malaria	[21]
	North-West Nigeria	Kirya, Ko-hi	Roots	Oral	Analgesic, anti-inflammatory	[22]
P. alba	Wichí people of Salta province, Argentina	Jwaayukw, Algarrobo blanco	Resin	Oral	Conjunctivitis, post-abortion pain	[23]
P. cineraria	Bahawalnagar, Punjab, Pakistan	Drucey	Leaves, stem	Oral	Spasm, diabetes, liver infection, diarrhea, removal of bladder and pancreas stone, fever, flu	[5]
				Topical	Rheumatism
	Thar Desert (Sindh), Pakistan	Gujjo	Fruit	Oral	Tonic for body, leucorrhea	[13]
	South of Kerman, Iran	Kahour	Fruit	Topical	Asthma, skin rash	[14]
	Pakistan	Unspecified	Flower	Oral	Rheumatism	[15]
	Hafizabad district, Punjab, Pakistan	Jhand	Leaf, bark, stem, flower, fruit	Oral, topical, eye drop	Liver tonic, boils and blisters, scorpion bite, pancreatic stone, leucorrhoea, chronic dysentery, cataract	[3]
	Pakistan	Unspecified	Fruit, pods	Unspecified	Asthma	[4]
	Pakistan	Jandi, Kanda, Kandee, Jhand	Leaves, Bark, Flowers, Pods and wood	Oral	Menstrual disorders, contraceptive, prevention of abortion	[16]
P. farcta	Jahrom, Iran	Kourak	Fruit	Oral	Constipation, febrifuge	[24]
P. glandulosa Torr	Bustamante, Nuevo León, Mexico	Mezquite	Inflorescences	Oral	Stomach pain	[25]
P. juliflora	Thar Desert (Sindh), Pakistan	Devi	Leaves, Gum	Oral	Painkiller, boils opening, eye inflammation, body tonic, muscular pain	[13]
	Isoschaftoside (23.67 mg/g)
	Hafizabad district, Punjab, Pakistan	Mosquit pod	Whole plant, Flower, Stem, Leaves, Bark	Oral, topical, and as toothbrush	Galactagogue, kidney stones, toothache, breast cancer, asthma, boils	[3	Schaftoside (14.86 mg/g)
]
Pakistan	Unspecified	Xerophytic shrub	Unspecified	Vitexin (0.46 mg/g)
Isovitexin (2.09 mg/g)
Asthma, cough	[	4	]
Mohmand Agency, FATA, Pakistan	Kikrye	Leaves	Oral	Lactation, expectorant	[17]
Western Madhya Pradesh, India	Reuja	Stem bark	Oral	Asthma	[18]	P. alba
P. nigra	Wichí people of Salta province, Argentina	Wosochukw, Algarrobo negro	Resin	Oral	Ocular trauma, conjunctivitis		[23]	exudate gum
P. ruscifolia	Wichí people of Salta province, Argentina	Atek, Vinal	LeavesFerulic acid 4-glucuronide (E)	[11]
Apigetrin, chrysin (E)
Chlorogenic acid (E and NE)
3-O-feruloylquinic acid (E)
p-Coumaroylquinic acid (E)
Valoneic acid dilactone (E)
Digallic acid (E)
Ferulic acid (NE)
Esculetin derivative (NE)
7-O-Methylapigenin (NE)
P. nigra pods	Cyanidin rhamnosyl hexoside	[10]
	Cyanidin-3-hexoside
	Peonidin-3-hexoside
	Malvidin dihexoside
	Cyanidin malonoyl hexoside
	Petunidin-3-hexoside
	Malvidin rhamnosyl hexoside
	Malvidin-3-hexoside
	Vicenin II
	Q-dihexoside rhamnoside
	Isoschaftoside
	Q-dihexoside
	Schaftoside
	Q-hexoside rhamnose
	K-hexoside rhamnoside
	Isovitexin
	Q-hexoside
	K-hexoside
	Apigenin hexoside rhamnoside
	Q methyl ether hexoside rhamnoside
	Oral		K-methyl ether hexoside rhamnoside
P. nigra flour	Vicenin II (0.34 μg/mg)	[14]
	Schaftoside (0.24 μg/mg)
	Isoschaftoside (0.27 μg/mg)
	Isovitexin (0.81 μg/mg)
	Protocatechuic acid (0.33 μg/mg)
	Coumaric acid (8.16 μg/mg)
	Ferulic acid (4.47 μg/mg)
Conjunctivitis, stomachache, pimples/rash, scabies, callouses, fever, birth/postpartum pains, diarrhoea, pediculosis, otitis	[	23	]
P. spicigera	Pakistan	Unspecified	Bark, leaves, flowers	Unspecified	Asthma	P. cineraria	Protocatechuic acid (31.65 mg/g) Chlorogenic acid (22.31 mg/g)	[15][16][17][15,16,17]
Caffeic acid (6.02 mg/g)
Ferulic acid (9.24 mg/g)
Prosogerin A, B, C and D
β-sitosterol
Hentriacontane
Rutin
Gallic acid
Patulitrin
Luteolin
[	Spicigerin
4	P. laevigata	Gallic acid (8–25 mg/100 g)	[18]
Coumaric acid (335–635 mg/100 g)
Catechin (162.5 mg/100g)
Gallocatechin (340–648 mg/100 g)
Epicatechin gallate (10–71 mg/100 g)
Rutin (222.4–256.1 mg/100 g)
Morin (236.5 mg/100 g)
]		Naringenin (20 mg/100 g)
Luteolin (13 mg/100 g)
P. juliflora	4′-O-Methylgallocatechin	[19][20][19,20]
	(+)-catechins
	(-)-mesquitol
	Apigenin
	Luteolin
	Apigenin-6,8-di-C-glycoside
	Chrysoeriol 7-O-glucoside
	Luteolin 7-O-glucoside
	Kaempferol 3-O-methyl ether
	Quercitin 3-O-methyl ether
	Isoharmentin 3-O-glucoside
	Isoharmentin 3-O-rutinoside
	Quercitin 3-O-rutinoside
	Quercitin 3-O-diglycoside
P. glandulosa

3.1. Prosopis cineraria

P. cineraria is traditionally used as a medicine in different regions of Pakistan, including Bahawalnagar in Punjab Province, the Thar Desert (Sindh) and Hafizabad district in Punjab (Table 2). The oral or topical administration of P. cineraria leaves, stems, fruits, flowers, barks and pods is used for the treatment of spasms, diabetes, liver infection, diarrhea, bladder and pancreas stones, fever, flu, rheumatism, leucorrhea, boils, blisters, scorpion bite, chronic dysentery, cataract, asthma, sexually-transmitted infections, and gynecological complaints, including menstrual disorders, as contraceptive and to prevent abortion ^{[3][4][5][13][15][16]}[3,4,5,13,15,16]. Besides Pakistan, in the South of Kerman in Iran, P. cineraria flowers are also topically applied to treat asthma and skin rashes [14].

3.2. Prosopis juliflora

P. juliflora is commonly used in Pakistan, and can be either orally consumed or topically applied. Leaves, gum, whole plant, flower, stem and bark of P. juliflora are used as a painkiller, body tonic, galactagogue, and expectorant, or to treat boils, eye inflammation, muscular pain, kidney stones, toothache, breast cancer, asthma and cough in Thar Desert (Sindh), Bahawalnagar in Punjab province and Mohmand Agency of Federally Administered Tribal Areas (FATA) ^{[3][4][13][17]}[3,4,13,17]. In addition to Pakistan, the stem bark of P. juliflora is also consumed in western Madhya Pradesh, India for the treatment of asthma [18].

3.3. Prosopis africana

P. africana is commonly known as African mesquite, and is also traditionally used as a medicine. The oral administration of leaves and bark of this plant is used for the treatment of malaria in Sélingué subdistrict in Mali [19] and Nsukka Local Government Area in south-eastern Nigeria [21].

In addition to malaria, various parts of P. africana, including roots, leaves and bark, are also used as analgesic and anti-inflammatory in Guinea-Bissau [22] and for the treatment of pains, pregnancy-related conditions (childbirth, breastfeeding, newborn diseases), and skin inflammations (i.e., wounds, burns) in north-west of Nigeria [20].

3.4. Other Prosopis Plants

The resin of P. alba and P. nigra, and the leaves of P. ruscifolia have also been consumed by the Wichí people of Salta province in Argentina against conjunctivitis, post-abortion pain, ocular trauma, stomachache, pimples or rash, scabies, callouses, fever, birth or postpartum pains, diarrhea, pediculosis and otitisSuárez [23].

A study carried out in South America (Bustamante, Nuevo León, Mexico), also pointed out that the ingestion of P. glandulosa inflorescences may be useful to relief stomach pain [25]. In addition to the above, in Jahrom, Iran, P. farcta fruits have been consumed to prevent constipation and to reduce fever [24], whereas P. spicigera bark, leaves and flowers are used for the treatment of asthma in Pakistan [4].