Plasma Proteomics in Morquio-A Disease: Comparison
Please note this is a comparison between Version 1 by María L. Couce and Version 2 by Bruce Ren.

Mucopolysaccharidosis type IVA (MPS IVA) is a lysosomal disease caused by mutations in the gene encoding the enzymeN-acetylgalactosamine-6-sulfate sulfatase (GALNS), and is characterized by systemic skeletal dysplasia due to excessive storage of keratan sulfate (KS) and chondroitin-6-sulfate in chondrocytes. Although improvements in the activity of daily living and endurance tests have been achieved with enzyme replacement therapy (ERT) with recombinant human GALNS, recovery of bone lesions and bone growth in MPS IVA has not been demonstrated to date. Moreover, no correlation has been described between therapeutic efficacy and urine levels of KS, which accumulates in MPS IVA patients. 

  • biomarkers
  • enzyme replacement therapy
  • lysosomal disorders
  • proteomics

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1. Introduction

Mucopolysaccharidosis type IVA (MPS IVA, OMIM #253000) or Morquio A syndrome is an autosomal recessive disease caused by mutations in the GALNS gene. This molecular defect results in a deficiency in the enzyme N-acetylgalactosamine-6-sulfate sulfatase (GALNS, E.C.3.1.6.4) [1][2][3][1,2,3], which degrades the specific glycosaminoglycans(GAGs) keratan sulfate (KS) and chondroitin-6-sulfate (C6S) [4][5][6][7][4,5,6,7]. These GAGs accumulate in multiple tissues, mainly bone, cartilage, heart valves, and cornea, leading to progressive systemic skeletal dysplasia [8][9][8,9]. Significant non-skeletal manifestations, including respiratory disease, spinal cord compression, cardiac disease, impaired vision, hearing loss, and dental problems, have also been described in MPS IVA patients [10][11][12][13][10,11,12,13]. MPS IVA is more frequently associated with severe and extensive skeletal manifestations than the other MPS types. Specifically, hypermobility of the joints is a characteristic of MPS IVA that distinguishes this disease from other forms of MPS. Furthermore, cognitive involvement is generally absent in MPS IVA [14]. Among untreated patients, respiratory failure is the primary cause of death, which typically occurs during the second or third decades of life [15][16][15,16].
Currently, two therapies for MPS IVA are used in clinical practice: enzyme replacement therapy (ERT) with recombinant human GALNS; and hematopoietic stem cell transplantation (HSCT) [5][11][5,11]. ERT and HSCT are based on the cross-correction principle, where by lysosomal enzymes are taken up by the cells of deficient recipients and targeted to the lysosomes via the mannose-6-phosphate receptor. However, as described for other lysosomal storage diseases, ERT for MPS IVA suffers from several limitations, including the need for weekly infusions of 4–6 h; rapid clearance due to a short half-life (35 min in human, 2 min in mouse) [17][18][19][17,18,19]; a high price [19][20][19,20]; limited penetration of the avascular cartilage; and immunological responses against the infused enzyme [16][17][21][16,17,21]. Moreover, clinical trials have shown little improvements in terms of bone growth and skeletal dysplasia. There is no definitive therapy that markedly improves bone and cartilage lesions in MPS IVA [14][18][19][20][14,18,19,20]. Potential biomarkers proposed in recent studies include KS, C6S, and blood levels of collagen II [22][23][22,23]. Although clinical trials have studied urinary KS as a potential biomarker, it has not been demonstrated that decreases in this parameter correlate with clinical improvement. Urinary KS originates in the kidneys but does not reflect the involvement of bone or other relevant tissues in MPS IVA, and is considered a pharmacokinetic marker but not a surrogate biomarker. The identification of valid biomarkers of bone lesions to evaluate therapeutic efficacy thus remains an unmet need. While bone cartilage samples are the best samples in which to search for candidate biomarkers of MPS IVA, the objective of this study was to assess the validity of candidate plasma biomarkers proposed in previous studies and to search for new biomarkers. To this end, we carried out both quantitative and qualitative proteomic analyses of plasma samples from.
Within rare disease research, proteomics is a fast-growing field. Approaches based on liquid chromatography-tandem mass spectrometry (LC-MS/MS), which are used to search for biomarkers in other human diseases [24][25][26][27][28][24,25,26,27,28], are key tools used to study normal and affected tissues and body fluids (e.g., plasma) in rare diseases [29].
Disease states involve alterations in the expression levels, phosphorylation states, and post-transcriptional modifications of proteins. Therefore, proteomics techniques have been applied to the study of normal and pathological conditions to search for biological information that could further our understanding of pathophysiological mechanisms and identify novel disease biomarkers [26][28][30][26,28,30]. Specific disease biomarkers identified using proteomics techniques could help improve diagnostic and prognostic precision and serve as key tools for the evaluation of treatment outcomes [24][25][26][27][28][29][30][31][24,25,26,27,28,29,30,31].

2. Qualitative Analysis of Proteins in Plasma Samples

In this study, we identified all proteins in plasma samples using data-dependent acquisition (DDA)-LC-MS/MS. Subsequent analysis was limited to proteins identified with 1% error (i.e., those with a false discovery rate (FDR) < 1). To obtain the most representative proteins per group, we selected proteins identified in at least n-1 samples per group (where n is the number of samples per group). The Venn diagram in Figure 1 depicts the distribution of proteins in each of the 4 groups, and indicates those that were common to multiple groups.
Figure 1. Venn diagram showing the distribution of proteins identified in the qualitative proteomic analysis across each of the 4 study groups.
Once we determined the proteins characteristic of each group, we performed a functional analysis using the FunRich program. This analysis allows us to characterize the behavior of the proteins identified in each group. Comparison of ERT-a and ERT-b samples revealed a favorable evolution (i.e., values obtained in ERT-a or ERT-b were close to thoseobtained for control samples) for certain proteins. Next, we selected groups of proteins in which alterations are observed in MPS IVA, and analyzed their behavior in each study group. From these, we identified proteins involved in hemostasis, bone regeneration, reorganization of theextracellular matrix and collagen, the immune response, complement system, and oxidative stress.
Among proteins implicated in hemostasis (blood coagulation, fibrin clot formation, and blood coagulation intrinsic pathway), we observed that values obtained for ERT-a samples (i.e., those collected 6 days after the preceding enzyme infusion) were closer to those of control samples than to ERT-b samples (Figure 2a). This result suggests that blood levels of these proteins require several days to stabilize after ERT infusion. A similar effect was observed for proteins implicated in different aspects of hemostatic processes, including platelet activation, platelet aggregation, and platelet degranulation (Figure 2b).
Figure 2. Functional enrichment analysis using FunRich. Proteins identified in each the different groupswere submitted to molecular functional analysis according to their involvement inhemostasis (a) and platelet function (b). CG, control group; ERT-a, patients sampled before ERT; ERT-b, patients sampled 24 h after ERT; UG, untreated group.
Other proteins identified in the proteomic analysis were implicated in bone regeneration and collagen function. Analysis of the behavior of these proteins showed that ERT had a negative regulatory effect on some (including proteins involved in mineralization, ossification, and osteoblast differentiation) and a positive regulatory effect on others (bone resorption and regulation of bone mineralization) (Figure 3a). In the case of proteins that were negatively regulated, ERT required more than 24 h to affect protein expression. Conversely, in the case of positively regulated proteins (involved in bone resorption and osteoblast differentiation) plasma levels increased after treatment in the ERT-b group (Figure 3a).
Figure 3. Functional enrichment analysis using FunRich. Proteins identified in each the different groups were submitted to molecular functional analysis according to their involvement in: (a) processes that occur at the bone level; (b) collagen function (activity/processing/biosynthesis); (c) collagen-containing extracellular matrix. CG, control group; ERT-a, patients sampled before ERT; ERT-b, patients sampled 24 h after ERT; UG, untreated group.
Proteomic analysis of proteins involved in collagen function revealed values close to those of the control group for proteins related to a positive regulation of the collagen biosynthesis (Figure 4b) in the ERT-a group. Moreover, in the ERT-a study group, we observed decreases in the expression of proteins implicated in collagen biosynthesis and collagen fibril organization. The behavior of proteins involved in metabolic and catabolic processes was unaltered after ERT administration (data not shown).
A search for proteins that interact with collagen in the extracellular matrix identified 5 proteins; P02671, P02751 (fibronectin 1), P02675, P51884 (lumican), and P02679.
We detected a relatively high number of proteins involved in the immune response. The results of the functional analysis of these proteins are shown in Figure 4 and Figure 5. Figure 4a shows the results of functional enrichment analysis of proteins implicated in inflammation, including proteins related to interleukins (ILs) IL-1, IL-1β, IL-6, IL-8, and to interferon γ (TNF-γ). Proteins were grouped according to their participation in different processes, including the cellular response to interferon γ, the cellular response to IL-1, the cellular response to IL-6, positive regulation of IL-1 secretion, positive regulation of IL-1β secretion, negative regulation of IL-1β secretion, negative regulation of IL-6 production, and positive regulation of IL-8 biosynthesis. In all cases, we observed a reduction in the expression of the proteins of interest24 h after ERT administration (ERT-b), except for proteins involved in positive regulation of IL-1 secretion (no change observed after ERT). In UG we detected 1.79% of proteins related to IL-1, IL-1β, and TNF-γ. This corresponding value in the ERT-a group (1.25%) was closer to that observed in controls (1.23%). In the case of proteins related to IL-6 and IL-8 we detected values of 0.88% in UG,0.63% in ERT-a, and 0.61 in the control group.
Figure 4. Functional enrichment analysis using FunRich. Proteins identified in each the different groups were submitted to molecular functional analysis according to their involvement in the immune response: proteinsrelated tointerleukins (ILs) IL-1, IL-1β, IL-6, IL-8, and interferon γ (a); proteins involved in immunoglobulin production, positive regulation of B-cell activation, negative regulation of the inflammatory response, and leukocyte migration (b).
Figure 5. Functional enrichment analysis using FunRich. Proteins identified in each of the different groups were submitted to molecular functional analysis according to their involvement in immune processes: proteins involved in the classical or alternative complement activation pathways (a); proteins involved in the response to oxidative stress (b).
The results of the functional enrichment analysis of proteins involved in biological processes related tothe immune response, immunoglobulin production, positive regulation of B cell activation, negative regulation inflammatory response, and leukocyte migrationare shown in Figure 4b. In all cases, ERT increased the expression of proteins involved in each of these biological processes, except for negative regulation of the inflammatory response.
Functional enrichment analysis was also performed to identify protein partner expression between groups of proteins related to inflammatory processes (biological processes relating to (i) the complement system, including the classical complement activation pathway, (ii) general complement activation, and (iii) the alternative complement activation pathway). The results (Figure 5) reveal a positive effect of ERT, although levels of protein expression levels did not reach those observed in the control group (Figure 5a). Figure 5b shows the results of the functional enrichment analysis of proteins involved in the oxidative stress response. Treatment (ERT-a) resulted in protein levels similar to those observed in the control group for proteins involved in both complement activation and oxidative stress.

3. SWATH Quantitative Analysisof Proteins in Plasma Samples

Quantitative analysis of proteins using SWATH revealed significant alterations in the expression of multiple proteins in the ERT-a and ERT-b groups relative to the control group (Table 1).
Table 1. Changes in plasma protein expression detected by SWATH in any of the patient groups (UG, ERT-a, or ERT-b) relative to the control group.
Group Comparison Proteins with Altered Expression* Upregulated Proteins Downregulated Proteins
Protein Code Protein Name p-Value Fold Change
Upregulated in ERT-a
P0DJI8 SAA1 Serum amyloid A-1 protein 0.0487 Only ERT-a
Q13790 APOF Apolipoprotein F 0.0487 Only ERT-a
A0A0C4DH68 KV224 Immunoglobulin kappa variable 2-24
UG vs CG 14 12 2
ERT-a vs CG 8 5(2 only in ERT-a) 1
ERT-b vs CG 14 10(2 only in ERT-b) 1

Quantitative Analysis Using SWATH

Proteins that were upregulated or down regulated in each group may be relevant in MPS IVA disease. Differences in protein expression in the UG may reveal candidate biomarkers of disease. Proteins upregulated or downregulated in the ERT-a and ERT-b groups can be considered as markers of disease evolution as they were altered before treatment. We identified certain proteins that were downregulated by more than 100 fold, including AMBP, ASM3B, KV117, and ZBTB4 (highlighted in dark red in Figure 6 and Table 2).
Figure 6. Volcano plot showing the results of the SWATH analysis of plasma protein expression. Data are represented as the results obtained for the control group relative to all other groups (UG, ERT-a, and ERT-b). Graph shows the negative base 10 log of the p-value plotted against the base 2 log of the fold change for each protein included in the SWATH analysis. Changes were considered significant at p < 0.05 and fold change >1.5 compared with control group.
Table 2. Changes in plasma protein expression detected in all patient groups (UG, ERT-a, and ERT-b) relative to the control group. Protein expression is considered altered in cases in which p < 0.05 and the fold change >1.5 (upregulated proteins) or <0.6 (downregulated proteins).
Uniprot Code Protein

Code
Protein Name p-Value Fold Change
0.0109
6.1248
P05156 CFAI Complement factor I 0.0065 5.2409
A0A075B6I0 LV861 Immunoglobulin lambda variable 8-61 0.0386 3.1547
P43251 BTD Biotinidase 0.0301 2.8996
P04196 HRG Histidine-rich glycoprotein 0.0224 1.5688
Downregulated in ERT-a
P02747 C1QC Complement C1q subcomponent subunit C 0.0465 0.5069
Uniprot Code Protein Code Protein Name p-Value Fold Change
Upregulated in ERT-b
P60174 TPIS Triosephosphateisomerase 0.0487 Only ERT-b
O00391 QSOX1 Sulfhydryl oxidase 1 0.0487 Only ERT-b
A0A0C4DH67 KV108 Immunoglobulin kappa variable 1-8 0.0028 Only ERT-b
P0C0L5 CO4B Complement C4-B 0.0029 17.9592
O14791 APOL1 Apolipoprotein L1 0.0480 2.2700
P20742 PZP Pregnancyzone protein 0.0418 2.1171
P06396 GELS Gelsolin 0.0080 2.0649
P00751 CFAB Complement factor B 0.0184 2.0058
P19823 ITIH2 Inter-alpha-trypsin inhibitor heavy chain H2 0.0093 1.9167
P01023 A2MG Alpha-2-macroglobulin 0.0093 1.8574
P08697 A2AP Alpha-2-antiplasmin 0.0298 1.8165
Q14624 ITIH4 Inter-alpha-trypsin inhibitor heavy chain H4 0.0389 1.7428
P05546 HEP2 Heparin cofactor 2 0.0421 1.6111
Downregulated in ERT-b
P02766 TTHY Transthyretin 0.0224 0.2358
Downregulated in UG, ERT-a, and ERT-b
P01860 IGHG3 Immunoglobulin heavy constant gamma 3 3.30 × 10−5 Only CG
P02741 CRP C-reactive protein 8.93 × 10−3 Only CG
P02760 AMBP Protein AMBP LV321 Immunoglobulin lambda variable 3-217.75 × 10−5 0.00070.0008
4.6098 Q92485 ASM3B Acid sphingomyelinase-like phosphodiesterase 3b 4.80 × 10−6 0.0021
P01594 KV133 Immunoglobulin kappa variable 1-33 0.0182 3.5490 P01599 KV117 Immunoglobulin kappa variable 1–17 6.69 × 10−4 0.0027
P02652 APOA2 Apolipoprotein A-II 0.0197 2.8151 Q9P1Z0 ZBTB4 Zinc finger and BTB domain-containing protein 4 2.81 × 10−7 0.0086
P01591 IGJ Immunoglobulin J chain 0.0395 2.4479 P01780 HV307 Immunoglobulin heavy variable 3–7 8.23 × 10−6 0.0333
P63261 ACTG Actin, cytoplasmic 2 0.0304 2.2374 P22352 GPX3 Glutathione peroxidase 3 1.31 × 10
P00734−4 0.0349
THRB Prothrombin 0.0265 2.0770 P35542 SAA4
B9A064Serum amyloid A-4 protein 8.01 × 10−5 0.0368
IGLL5 Immunoglobulin lambda-like polypeptide 5 0.0420 1.9341 P01766 HV313 Immunoglobulin heavy variable 3–13 4.50 × 10−2 0.0402
P01714 LV319 Immunoglobulin lambda variable 3-19 0.0313 1.7496 P04004 VTNC Vitronectin 1.00 × 10−6 0.0417
P00742 FA10 Coagulation factor X 4.71 × 10−9 0.0535
P02042 HBD Hemoglobin subunit delta 2.79 × 10−5 0.0565
A0A075B6J9 LV218 Immunoglobulin lambda variable 2–18 8.30 × 10−4 0.0588
P02753 RET4 Retinol-binding protein 4 6.31 × 10−3 0.1103
P06312 KV401 Immunoglobulin kappa variable 4–1 2.12 × 10−6 0.1292
P02654 APOC1 Apolipoprotein C-I 7.01 × 10−6 0.1515
P02765 FETUA Alpha-2-HS-glycoprotein 6.60 × 10−5 0.1527
P69905 HBA Hemoglobin subunit alpha 1.86 × 10−5 0.1585
P02655 APOC2 Apolipoprotein C-II 8.27 × 10−5 0.1642
P00915 CAH1 Carbonicanhydrase 1 1.37 × 10−2 0.1984
P02751 FINC Fibronectin 9.79 × 10−3 0.2304
A0A0B4J1Y8 LV949 Immunoglobulin lambda variable 9–49 6.70 × 10−3 0.2353
P02746 C1QB Complement C1q subcomponent subunit B 1.68 × 10−3 0.2381
P00738 HPT Haptoglobin 3.00 × 10−3 0.2445
P01857 IGHG1 Immunoglobulin heavy constant gamma 1 2.37 × 10−2 0.2519
P01834 IGKC Immunoglobulin kappa constant 4.67 × 10−4 0.2591
P68871 HBB Hemoglobin subunit beta 1.01 × 10−3 0.3597
P02763 A1AG1 Alpha-1-acid glycoprotein 1 2.43 × 10−2 0.4115
Of the proteins that were the upregulated and downregulated in the UG, ERT-a, and ERT-b groups relative to controls, 31% were related to immune system function. It is important to point out that most of the proteins for which changes in expression were observed in the study groups were down regulated. In addition to upregulated protein in the UG, ERT-a, and ERT-b groups relative to CG, we alsodetected2 immunoglobulins (KV320 and LV321) that were not present in the CG.
Some of these downregulated proteins (highlighted in red in Table 2 and in the heatmap in Figure 7) may be of particular importance in MPS IVA.
Figure 7. Heatmap depicting the expression profiles of different proteins (also shown in Table 2, Table 3 and Table 4) as determined by SWATH quantitative analysis. The relative abundance of each protein is reflected by the colors in the heatmap, based on the z-score of the protein’s normalized peak area. The dendrogram represents the biological replicates of the UG, ERT-a, ERT-b, and CG groups. Proteins, listed in the sidebar on the right, are color coded according to their function, as follows: apolipoprotein-related proteins, dark green; blood coagulation, purple; calcium binding, dark orange; carrier proteins, light green; cytoskeletal proteins, light pink; immune system, grey; metabolic processes, yellow; metabolic interconversion, light blue; other functions, white; protein binding, pink; transport, light grey.
Table 3. Changes in protein expression in the untreated group relative to all other groups. Protein expression is considered altered in cases in which p < 0.05 and the fold change >1.5 (upregulated proteins) or <0.6 (downregulated proteins).
Uniprot Code Protein Code Protein Name p-Value Fold Change
Upregulated in Untreated Group
P06702 S10A9 Protein S100-A9 0.0036 17.4468
P01615 KVD28 Immunoglobulin kappa variable 2D-28 0.0008
0.2681
P01859
IGHG2
Immunoglobulin heavy constant gamma 2
3.60 × 10
−4
0.2959
P29622
KAIN
Kallistatin
1.99 × 10
−3
0.3344
P02743 SAMP Serum amyloid P-component 1.91 × 10−2 0.3509
P02768 ALBU
P01009 A1AT Alpha-1-antitrypsin 0.0014 1.6875
P02747 C1QC Complement C1q subcomponent subunit C 0.0223 1.6522
Downregulated in Untreated Group
P02746 C1QB Complement C1q subcomponent subunit B 0.0329 0.1549 Serum albumin
P08697 A2AP Alpha-2-antiplasmin 0.0275 0.4938 1.92 × 10−5 0.3509
P02787 TRFE Serotransferrin 4.11 × 10−2 0.3571
P02749 APOH Beta-2-glycoprotein 1 1.26 × 10−2 0.3584
P02679 FIBG Fibrinogen gamma chain 5.71 × 10−3 P05543 THBG Thyroxine-binding globulin 4.82 × 10−4 0.4348
Upregulated in UG, ERT-a, and ERT-b
P01619 KV320 Immunoglobulin kappa variable 3–20 0.0005 Not found CG
P80748 LV321 Immunoglobulin lambda variable 3-21 0.0179 Not found CG
B9A064 IGLL5 Immunoglobulin lambda-like polypeptide 5 0.0002 44.0529
P01615 KVD28 Immunoglobulin kappa variable 2D–28 0.0474 27.4725
P0DOY2 IGLC2 Immunoglobulin lambda constant 2 0.0217 26.1780
P35858 ALS Insulin-like growth factor-binding protein complex acid labile subunit 0.0299 10.8460
P10909 CLUS Clusterin 0.0010 10.1523
P02750 A2GL Leucine-rich alpha-2-glycoprotein 0.0004 9.9800
P01023 A2MG Alpha-2-macroglobulin 0.0000 4.7170
P00734 THRB Prothrombin 0.0132 4.3178
P06396 GELS 0.0057 3.3223
5.6619 Gelsolin P19823 ITIH2 Inter-alpha-trypsin inhibitor heavy chain H2 0.0023 3.1536
Q96IY4 CBPB2 Carboxypeptidase B2 0.0121 2.8977
Q08380 LG3BP Galectin-3-binding protein 0.0361 2.5880
P08185
P80748 CBG Corticosteroid-binding globulin 0.0325 2.2763
A0A0A0MS15 HV349 Immunoglobulin heavy variable 3–49 0.0482 2.1418
A0A0C4DH38 HV551 Immunoglobulin heavy variable 5–51 0.0165 2.1345
Table 4. Changes in protein expression in the ERT-a or ERT-b groups relative to all other groups. Protein expression is considered altered in cases in which p < 0.05 and the fold change >1.5 (upregulated proteins) or <0.6 (downregulated proteins).
Uniprot Code
In order to shown the results in a better way, we represent a heatmap using the proteins up and down regulated shown in Table 2. Moreover, the proteins were classified into different functions and were highline in different colors. Among these proteins, those shown in Figure 8 and Figure 9 stand out.
Figure 8. Box plot depicting expression levels of the6 most relevant plasma proteins (ALBU, HBA, FETUA, APOC1, APOC2, VTNC, and GPX3) for which downregulation was observed in the UG and ERT-a and ERT-b groups relative to the control group (CG). Each data point represents the median protein expression value obtained in an individual sample. The line inside the box represents the median of all values obtained. The upper and lower limits of the box represent the first and third quartiles. Whiskers represent the minimum and maximum values within 1.5 times the interquartile range. Any data points not included between the whiskers are considered outliers. * p < 0.05; ** p < 0.01; *** p < 0.001.
Figure 9. Box plot depicting expression levels of the 5 most relevant plasma proteins (RET4, SAA4, FA10, FINC, and C1QB) for which very low expression was observed in the UG and ERT-a and ERT-b groups relative to the control group (CG). Each data point represents the median value obtained in an individual sample. The line inside the box represents the median of all values obtained. The upper and lower limits of the box represent the first and third quartiles. Whiskers represent the minimum and maximum values within 1.5 times the interquartile range. Any data points not included between the whiskers are considered outliers. * p < 0.05; ** p < 0.01; *** p < 0.001.
The SWATH analysis identified proteins that were downregulated in the UG and the ERT-a and b groups relative to controls, or were absent entirely. These included FA10 and SAA4, which were absent in all patient groups, and RET4 and C1QB, which were absent in UG and in UG and ERT-a, respectively (see Figure 9 and Figure 10).
Figure 10. Box plot depictingthe4 plasma proteins (A2GL, CLUS, GELS, ITIH2) with the highest expression levels in the UG and ERT-a and ERT-b groups relative to the control group (CG). Each data point represents the median value obtained in an individual sample. The line inside the box represents the median of all values obtained. The upper and lower limits of the box represent the first and third quartiles. Whiskers represent the minimum and maximum values within 1.5 times the interquartile range. Any data points not included between the whiskers are considered outliers. * p < 0.05; ** p < 0.01; *** p < 0.001.
In the UG group, we detected changes in expression in 4 proteins with respect to the 3 other groups (Figure 11 and Table 3).
Figure 11. Volcano plot showing the results of the SWATH analysis of plasma protein expression. Data are represented as the results obtained for the untreated group (UG) relative to all other groups (CG, ERT-a, and ERT-b). Graph shows the negative base 10 log of the p-value plotted against the base 2 log of the fold change for each protein included in the SWATH analysis. Changes were considered significant at p < 0.05 and fold change >1.5.
Because these proteins were likely dysregulated as a consequence of the disease, they may include potential disease biomarkers that are either up-or down regulated in MPS IVA.
Theproteinsfor which changes in expression were detected in the UG included severalimmunoglobulins. This is unsurprising, given the important inflammatory component of MPS. Moreover, proteins that were dysregulated in this group included some previously proposed as biomarkers of MPS (e.g., A1AT[alpha 1 antitrypsin]) [32][33].We also observed dysregulation of proteins involved in inflammation (S10A9), binding to collagen (C1QC), and prothrombin (THRB), a thyroid hormone receptor that mediates the biological activities of thyroid hormone [33][34]. Proteins that were downregulated in the UG included C1QB (in line with previously reports, Figure 8) and the serine protease inhibitor A2AP (Figure 9 and Table 4).
The most important dysregulated protein identified in this analysis was A1AT. Analysis of A1AT levels in individuals in each of the different groups (Figure 12) revealed significant upregulation in the UG compared with each of the other groups. A1AT levels in the ERT-a and ERT-b groups were comparable to those observed in controls. This protein may constitute not only a biomarker of MPSIVA, but also an indicator of disease progression, potentially providing a better means of monitoring treatment responses in MPS IVA.
Figure 12. Box plot depicting ATA1 levels in each of the study groups. Each data point represents the median value obtained in an individual sample. The line inside the box represents the median of all values obtained. The upper and lower limits of the box represent the first and third quartiles. Whiskers represent the minimum and maximum values within 1.5 times the interquartile range. Any data points not included between the whiskers are considered outliers. * p < 0.05.
While treatment resulted in changes in the expression of proteins in both the ERT-a and ERT-b groups (Table 4), none of these proteins were common to both groups.