Mycosporine-like amino acids, commonly known as “MAAs” represent a diverse family of more than 40, small <400 Da, water-soluble, colorless UV-absorbing compounds that protect against highly energetic UV photons. They have a unique absorption spectrum with a single, narrow band with an absorption maximum between 309 and 362 nm. Structurally, MAAs are divided into two groups; (i) the mycosporines, which have a single modified amino acid residue connected to a cyclohexenone core, and (ii) MAAs, have two such amino acids substituents [71]. MAAs have a 5-hydroxy-5-hydroxymethyl-cyclohex-1, 2-ene ring structure, and a methoxy-substituent in C2 position. In the MAA structure, the C3 position is always substituted with an amino group, whereas the C1 position can be substituted with either an oxo- or an imino-moiety. In some instances, the term ‘mycosporine’ refers to those with a ketone group at the C1 position, known as oxo-mycosporines or mono-substituted mycosporines.
The presence of MAAs has been reported in a wide range of living organisms, including invertebrates and vertebrates such as cyanobacteria, red and green algae, dinoflagellates, fungi, lichens, corals, sponges, sea urchins, scallop and fish [
]. Nevertheless, the evolution of UV-screening compounds is a subject that has not been extensively studied. Mulkidjanian and Junge, [
] hypothesized that aromatic-groups bearing reaction center compounds were the earliest UV-screens that subsequently started to perform a light-harvesting role in photosynthesis. The mechanism behind the origin of UV-screening compounds is still undiscovered, although it is assumed that initially, many photoprotective compounds evolved for other biological functions but later developed UV-screening functions under selection pressure [
]. Besides the photoprotective role, MAAs also functioned as osmotic regulators, especially where a hypersaline environment surrounded the cyanobacterial cell. Probably, such roles may have given rise to the first UV screening MAAs [
]. To provide the necessary osmotic balance, most cyanobacteria accumulate MAAs as “osmotic solutes” or “compatible solutes” in the cell’s intracellular space, which builds osmotic pressure within the cell. MAAs naturally accumulate as solutes in the cytoplasm, but their derivatives, covalently bound to oligosaccharides, can be excreted into the enveloping ‘sheath’ (glycocalyx), as in
]. Generally, MAA production is stimulated by UV-B radiation. Nevertheless, it is suggested that as oxygen levels increased in cells, UV-A screening, mainly with di-substituted MAAs, became important because most of the effects of UV-A are mediated through oxygen-free radicals [
]. This change in the absorption spectrum from UV-B to UV-A can be achieved by replacing the ketone group with a nitrogen atom in UV-B absorbing compounds. This has a more significant mesomeric effect on the benzene ring, and absorbance is shifted into the UV-A region. A mutation in a UV-B screening compound’s proposed biosynthetic pathway may have also caused a shift towards UV-A absorption [
]. In cyanobacteria, the MAA content can be correlated with a moderate physiological amelioration of photo-damage, which persists even under conditions of physiological inactivity, as expected for a sunscreen [
]. Exposure to intense solar radiation induced or enhanced the accumulation of MAAs in most of the MAA-synthesizing organisms, although the exact action spectrum for their responses varies [
].
The common mono- and di-substituted MAAs identified from cyanobacteria are listed in with their molecular weights, molar extinction coefficients and absorption maxima [
]. Because cyanobacteria arose in the Precambrian era and have been exposed to high evolutionary pressure, they had the potential to produce MAAs, which, in contrast to other organisms, protect them against diverse environmental conditions. The occurrence of MAAs has been recorded in all diverse habitats, especially in those that are exposed to extensive solar radiation, desiccation, as well as high temperature and other stresses. Similarly, cyanobacteria exposed to intense solar radiation such as in paddy fields, on the bark of trees, roof-tops etc., have been isolated and have been shown to contain mycosporine-Gly and shinorine, with a role in photoprotection [
]. Some common types of MAAs such as asterina-330, shinorine, and mycosporine-Gly have been reported to play a significant photoprotective role in freshwater cyanobacteria of the high-mountain lakes situated in Austria’s Central Alps [
]. Similarly, the bloom-forming cyanobacterium,
synthesizes shinorine and porphyra-334, which absorb UV-B radiation and thus allows the cyanobacterium to develop and maintain surface blooms, even in the presence of high solar irradiance, including ultraviolet radiation [
]. Oren [
] first reported the presence of high levels of MAAs in the halophilic cyanobacterial community [
]. They found a very high intracellular concentration of about 98 mM MAAs in a community of unicellular cyanobacteria inhabiting a gypsum crust developing on the bottom of a hypersaline saltern pond in Eilat, Israel. Rastogi et al. [
] showed the presence of different MAAs in a cyanobacterial mat collected from the old temple located in the Phra Nakhon district, Bangkok, Thailand. The cyanobacterial mat was found to contain shinorine, porphyra-334, mycosporine-Gly, and palythiol, along with two unknown MAAs having absorption maxima at 320 and 329 nm. The mat included the presence of
sp. [
]. Singh et al. [
] have described the presence of mycosporine-Gly, shinorine and a few unidentified MAAs in different cyanobacterial crust samples collected from the rice-field, roof-top and bark of trees growing on the campus of Banaras Hindu University, Varanasi, India [
].
The accumulation of MAAs in vertebrates such as fish is the best example of symbiosis. Fish lack the appropriate biosynthetic pathways and therefore accumulate MAAs via their algal diet or by bacterial or algal symbionts. Besides the dietary source, de novo synthesis of the MAA precursor compound, gadusol, was observed in coral and fish [
]. The
coral has a wide range of MAAs including mono- and di-substituted MAAs such as mycosporine-Gly, porphyra-334, shinorine, mycosporine-methylamine-serine, palythine-serine, mycosporine-methylamine-threonine, palythine, and palythine-threonine [
].
Earlier studies suggested that the biosynthesis of MAAs arose in fungi and cyanobacteria from the first part of the shikimate pathway, probably directly from 3-dehydroquinate synthase (DHQ synthase encoded by aroB) via 4-deoxygadusol [
]. Studies on
sp. revealed that condensation of the cyclohexenone ring, in terms of amino acids, could lead to the formation of new substituents for MAAs. Mycosporine-Gly is the first oxo-mycosporine converted into imino-mycosporines by chemical or biochemical modifications, more precisely via amino acid substitution [
]. This is depicted in showing how mycosporine-Gly was the first MAA synthesized, and this acted as a precursor for other mono- and bi-substituted imino-mycosporines, such as mycosporine-glycine-valine, shinorine and porphyra-334 [
].
A study of comparative genomics of four cyanobacteria has simplified the identification of the MAA biosynthetic gene locus [
]. Genome mining revealed that
sp. PCC 7120 had two sets of the 3-dehydroquinate synthase (DHQS) gene, whereas
sp. PCC 6803 and
sp. PCC 6301 had a single set of this gene in their genome. In
PCC 7937, the cyclohexenone core was possibly formed by dehydroquinate (DHQ) synthase homologues (locus: Ava_3858), which are flanked by a putative O-methyltransferase (O-MT) (locus: Ava_4386) [
]. Likewise, the cyanobacterium
ATCC 29413 a known producer of shinorine possesses a putative gene cluster consisting of four ORFs. It is thought that the DHQS homolog Ava_3858 and O-MT Ava_3857 could assemble into 4-deoxygadusol (4-DG), which is a cyclohexenone core product and precursor of mycosporine. Other two ORFs such as the ATP-grasp homolog Ava_3856 and the NRPS-like enzyme Ava_3855, perhaps accountable for the assignment of glycine and serine to precursor molecules via imine linkages.
Another type of biosynthetic pathway was identified by studying
gene products, where 4-deoxygadusol was produced in vitro when the culture was supplemented with sedoheptulose phosphate (SHP) as a substrate, but not when supplied with 3-DHQ. In
a demethyldeoxygadusol synthase (DDG synthase) was encoded by NpR5600 and Ava_3858, although demethyldeoxygadusol methyltransferase (O-MT) were encoded by Ava_3857 and NpR5599, respectively. These results show that this pathway branches out from central metabolism rather than from the early shikimate pathway. The Ava_3858/Ava_3857 couple and Ava_3856, a three-gene core, is highly conserved in cyanobacteria, as well as in fungi, and is essential for the biosynthesis of a mono-substituted MAA (mycosporine-Gly) from central metabolites (Gly and sedoheptulose-6-phosphate (SHP). Heterologously expressed Ava_3855 formed shinorine from mycosporine-Gly and serine in vitro, and the Ava_3855 enzyme is likely to activate serine carboxylate to catalyze imine formation. However, it remains to be seen how the cyanobacteria that lack Ava_3855 homologues achieve the synthesis of imino-mycosporines [
].
Identification and characterization of MAAs have been accomplished by using several techniques that can determine their unique structure. The most commonly used methods for structural determinations of MAAs are UV-Vis spectroscopy, HPLC, ESI-MS, LC-MS, FTIR, NMR spectra such as 1D (1H and 13C) and 2D (COSY, NOSY and HMBC). A few other methodologies such as chemical assays (amino acid composition after alkaline hydrolysis, methylation and interchange H/D), melting point (m.p.) determination, elemental analysis, optical activity, and X-ray diffraction have also been used to characterize MAAs [
]. As mentioned earlier, one of the most significant features of MAAs is the occurrence of a unique, strong absorption peak in the UV region. Due to this exceptional characteristic feature, extracted MAAs are commonly characterized by diode-array detection (DAD) followed by HP-TLC plates or HPLC/UPLC columns [
]. Sometimes characterization and quantification of MAAs are performed via reverse-phase DAD columns with a TFA/ammonium mobile phase to increase the polarity separation of MAA mixtures [
]. Over recent decades, several mass spectroscopic techniques have been used to elucidate the structure of MAAs [
], especially since the analysis of fragmentation patterns has become more reliable. NMR always remains the method of choice for the prediction of MAA structures. These are well-conserved, consisting of a cyclohexenone or cyclohexenimine ring substituted with amino alcohols or amino acids [
]. The characteristic absorption peak of FTIR spectra of MAA indicates the presence of several functional groups such as hydroxyl, amino (NH
]. The crystal, molecular structure and absolute configuration of palythene and palythine were unambiguously determined by X-ray analysis [
]. Recently, Orfanoudaki et al. [
] determined the crystal structure of a shinorine hydrate from single-crystal X-ray diffraction and its absolute configuration was established from anomalous-dispersion effects. Along with this, they have also determined the absolute configuration of 14 MAAs by combining the results of electronic circular dichroism (ECD) experiments with advanced Marfey’s method using LC-MS.
3.5. Mycosporine-Like Amino Acids and Their Applications
MAAs are considered multipurpose metabolites with various functions, including antioxidant and anti-inflammatory activities, accessory pigments in photosynthesis, nitrogen storage, thermal protection, osmotic stress protection, anti-aging, anti-cancer, and wound healing. They have also been widely accepted as UV-A and UV-B photoprotection agents [93]. The evidence supporting these functions of MAAs is mostly indirect and is based on induction of MAA production following stress conditions. MAAs have well-documented applications in cosmetics, toiletries, as UV protectors, and activators of cell proliferation and as suppressors of UV-induced aging in human skin [66,67]. MAAs appear to be promising compounds in artificial sunscreens for future biotechnological research [117]. Some of their properties are briefly discussed below, and shows a diagrammatic representation MAA applications.
Figure 4. A scheme to represent MAA-producing organisms, biological functions of MAAs and their cosmetic applications.
3.5.1. Antioxidative Properties of MAAs
UV radiation interacts with oxygen and other organic compounds and can generate oxidative stress by producing highly reactive oxygen intermediates such as superoxide (O2.-), hydroxyl radical (OH) or hydrogen peroxide (H2O2). To counteract the damaging effects of oxidative stress, cyanobacteria exhibit several defence mechanisms, such as the production of MAAs [68]. Several in vitro analyses of MAAs suggest that different abiotic stresses such as temperature, salinity, desiccation and acidity may significantly increase their antioxidative properties [81,93,118]. Oren [94] has clearly described an osmotic role of MAAs in a hypersaline environment. The antioxidative activities of mycosporine-Gly and shinorine have been very effective against ROS scavenging, although mycosporine-Gly has the highest antioxidative activity among several naturally occurring compounds. Compared with ascorbic acid, an eight-fold higher antioxidant activity has been reported at pH 8.5 for mycosporine-Gly, which was isolated from the marine lichen Lichina pygmaea [84,119]. The activity of porphyra-334, as an antioxidant on human skin fibroblasts, was also studied, and results showed a dose-dependent reduction in intracellular UV-A-induced ROS generation based on a modified DCF-DA fluorescence assay [119]. Recently, Kageyama and Waditee-Sirisattha [118] compiled a list of a few mono- and di-substituted MAAs as ROS scavengers, both in vitro and in vivo. Mycosporine-2-glycine has antioxidative effects in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages by down-regulated Sod1, Cat, and Nrf2 expression [120]. Ryu et al. [119] showed that treatment of porphyra-334 decreased the UV-A-induced intracellular ROS generation in human skin fibroblasts in a dose-dependent manner. Likewise, palythine isolated from the red alga Chrondus yendoi significantly reduced SSR-irradiated (20 J cm?2) production of oxidizing species in Ha- CaT immortal human keratinocytes [68]. Recently, RNA-sequencing analysis of human follicle dermal papilla (HFDP) cells following treatment with porphyra-334 proved the antioxidant role of porphyra-334 by upregulating metallothionein (MT)-associated gene expression [121].
3.5.2. Anti-Inflammatory Properties of MAAs
Inflammation is a physiological defense mechanism to fight against molecular and cellular damage caused by oxidative stress or UV irradiation. UV mediated inflammatory responses includes synthesis of inducible NO synthase (iNOS), nitric oxide (NO),
cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2), tumor necrosis factor- (TNF- ), and other cytokines, such as interleukin-1 (IL-1) and interleukin-6 (IL-6) [118]. Expression of the COX-2 protein, linked with PGE2 production, is upregulated by UV-B exposure and ROS generation in both human skin and cultured human keratinocytes [118]. Suh et al. [69] studied the anti-inflammatory response of mycosporine-Gly, porphyra-334 and shinorine against UV exposure on HaCaT cell lines. They evaluated expression levels of the COX-2 gene (linked with tissue inflammation) under three different concentrations of MAAs (0.03, 0.15, or 0.3 mM). Results showed that mycosporine-Gly and shinorine can inhibit the expression of the COX-2 gene, whereas porphyra did not show any significant effect [69]. Becker et al. [122] studied the immunomodulatory effects of shinorine and porphyra-334 in the human myelomonocytic cell line THP-1 and their descendent reporter line THP-1-Blue by observing activation of transcription factor NF-B. Both cells were exposed to the MAAs in the presence or absence of lipopolysaccharide (LPS). They observed that both MAAs had immunomodulatory effects on NF-B activity in unstimulated THP-1-Blue cells, whereas the activity of NF-B was increased by shinorine in a more pronounced and dose-dependent manner. In contrast to this, the activity of NF-B was reduced following porphyra-334 treatment and confirmed its anti-inflammatory potential. Likewise, Tarasuntisuk et al. [120] also reported anti-inflammatory activity in mycosporine- 2-glycine lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Transcriptional analyses of this study showed that mycosporine-2-glycine significantly repressed the expression of iNOS and COX-2. Consequently, it also inhibited the generation of inflammatory intermediaries by suppressing the NF-B pathway. Similarly, aqueous extracts of Gracilariopsis longissima and Hydropuntia cornea induced both TNF- and IL-6 production in macrophages of cell line RAW264.7. These results demonstrate the anti-inflammatory properties of MAAs, particularly palythine, asterina-330, shinorine, porphyra-334, and palythinol, which are present in cell extracts of H. cornea and G. longissima [123].
3.5.3. Anti-Aging and Wound Healing Properties of MAAs
Prolonged UV exposure leads to skin aging by degrading collagen fiber and reducing elastin content. Like anti-inflammatory activity, Suh et al. [69] also demonstrated MAAs efficacy against aging. They found that UV irradiation strongly suppressed expression of elastin and the procollagen c-endopeptidase enhancer (PCOLCE) gene, which binds to procollagen and enhances procollagen c-proteinase activity. However, the presence of mycosporine-Gly, porphyra-334, and shinorine elevated the UV-suppressed levels of PCOLCE and elastin in a concentration-dependent manner and also showed their wound healing properties [69]. Recently, Rui et al. [124] studied the anti-aging properties of a mixture of porphyra-334 and shinorine on ICR mice and demonstrated that MAAs havethe potential to suppress collagen and elastin degradation; this could therefore be an
effective treatment against skin aging. Similarly, Ryu et al. [119] showed that porphyra- 334 suppressed ROS generation and the expression of matrix metalloproteinases (MMPs) that are linked with connective tissue degradation during photo-aging. At the same time, it increases the levels of procollagen, type I collagen and elastin to help maintain healthy skin cell and the healing of fibroblasts [119]. They found that porphyra-334 can suppress the expression of MMP in a dose-dependent manner. The highest concentration of porphyra-334 (40 M) inhibited up to 56.2% MMP-1 mRNA expression in UV-A exposed human skin fibroblasts [119]. Recently, Kim et al. [121] proved the role of porphyra-334 as an anti-aging agent, including the promotion of collagen formation, improvement of periorbital wrinkles, and promotion of cell proliferation, in the human cell lines human Detroit 551 fibroblast cells, HaCaT cells, and HFDP cells derived mainly from normal human scalp hair follicles [121]. They observed that procollagen expression levels (PIP) increased in Detroit 551 cells with increasing levels of porphyra extract and porphyra-334. One ppm of porphyra extract and porphyra-334 increased the PIP content by 121% and 130%, respectively. In contrast, 10 ppm of porphyra extract and porphyra-334 increased PIP content by 147% and 154%, respectively. The in vitro efficacy of few common MAAs against UV-induced damage are listed in Table 2.
3.5.4. Photo-Protective Properties of MAAs
Over recent years, the application of MAAs in sunscreen products has attracted increasing interest. Various properties of MAAs, such as strong UV-absorption maxima (310–365 nm), high molar extinction coefficients (" = 28,100–50,000 M?1cm?1), photostability, ability to prevent UV-induced thymine dimer formation and resistance to several abiotic stressors demonstrates that MAAs are potent photo-absorbing compounds [72,73,117]. The ability of MAAs to absorb UV radiation and dissipate energy as heat without generating reactive photoproducts makes it significant photoprotective compounds (Figure 5). Torres et al. [86] demonstrated the UV-B photoprotective activity of a novel mycosporine collemin A isolated from Collema cristatum, a lichenized ascomycete [86]. The photoprotective roles of porphyra-334, shinorine, and mycosporine-Gly isolated from Patinopecten yessoensis ovaries has been studied in human skin fibroblast cells by Oyamada et al. [80]. They found that all three MAAs can protect the cells against UV-induced cell death, but the most substantial effect was shown by mycosporine-Gly. Besides this, MAAs also promoted the proliferation of human skin fibroblast cells [80]. Lately, another interesting study conducted by de la Coba et al. [125] showed that natural sunscreen formulations combining porphyra-334 and shinorine act nearly as well as OMC synthetic UV-A filters and mycosporine-serinol as a UV-B filter. The results showed that each natural and artificial sunscreen formulation exhibited comparable SPFs when applied in identical concentrations as UV-A and UV-B filters [125].
Similarly, Moline et al. [85] showed that the photoprotective activity of yeast involved the synthesis of mycosporine-glutaminol glucoside (MGG). In this work, they analyzed the relationship between MGG production, cell survival after UV-B irradiation, formation of CPDs, photostability and singlet oxygen quenching activity of MGG [85]. Their results showed that CPD accumulation and MGG accumulation were inversely related. The conclusion of their work was that MGG plays an important role as a UV-B photoprotective metabolite in yeasts by protecting against direct DNA damage and probably against indirect damage by singlet oxygen quenching. Likewise, porphyra-334 isolated from Porphyra yezoensis exhibited a protective effect on human skin fibroblasts against exposure to UV-A radiation. Cell viability was increased in a dose dependent manner similar to 40 Mporphyra-334 that increased cell viability by up to 88% [119]. Recently, Suh et al. [70] used porphyra-334 to minimize the UV-induced apoptosis of HaCaT cell lines. Likewise, they also showed that UV-absorbing compounds (M-Gly, shinorine and porphyra-334) modulated gene expression associated with oxidative stress, inflammation, and skin aging caused by UV [69]. Álvarez-Gómez et al. [123] have investigated the effect of algal cellextracts of G. longissima and H. cornea on two human and one murine cell lines and found that they had no cytotoxic effects on human cell lines. Nevertheless, murine cell lines exhibited cytotoxic effects linked to immunomodulatory roles. The algal extracts included five different MAAs: palythine, asterina-330, shinorine, porphyra-334, and palythinol. They also found that the photoprotective capacity of the algal extracts in terms of SPF values showed a gradual increase with extract concentration. Both algal extracts induced the production of TNF- and IL-6 [123].
Figure 5. Use of natural eco-friendly mycosporine-like amino acids (MAAs) as a green sunscreen to protect skin against UV-induced skin damage. Created with BioRender (https://biorender.com/ accessed on 15 April 2021).
4. Stability and Enhanced Effectiveness of MAA-Based Sunscreens, and MAA-Conjugates
Fernandes et al. [126] made an effort to enhance the UV protective properties of MAAs by grafting them with a chitosan (CS) matrix through amide bond formation based on carbodiimide coupling. Results showed that CS-MAA conjugates, as an extraordinarily stable combination of natural biological molecules, exhibited various benefits such as being biodegradable, biocompatible, thermoresistant, photoresistant, and with increased efficacy against both UV-A and UV-B radiation compared to individual MAAs [127]. This provides an opportunity to further engineer conjugates to generate new multifunctional materials through the modification of several remaining free amino groups on a CS matrix. It also fulfills the current requirements for cosmetic products or biopharmaceutical agents because the carbodiimide-based grafting procedure and products are already used extensively in these fields. This study provides more emphasis on the applicability of CS-MAA conjugates to a wide range of applications in fields such as cosmetics, artificial skin, wound healing, contact lenses, artificial cornea, textiles, food, drug packaging, and coatings [126]. Similarly, Singh et al. [79] have attempted to synthesize a stable ZnONPs conjugate with the UV-absorbing compound shinorine (10 mM concentration) at pH-7. They found that ZnONPs shinorine conjugate treatment reduced in vivo ROS generation by up to 75% in Anabaena strain L31. Schmid et al. [128] introduced a Helioguard®365 commercialized natural sunscreen formulation of porphyra-334 and shinorine, extracted from the red alga Porphyra umbilicalis. Helioguard®365 has anti-aging as well as photoprotective capacity against UV-A induced skin or DNA damage. It improved cell viability in a dose-dependent manner; for example, 0.25% Helioguard®365 increased cell viability by up to 97.8%. In addition, 3% and 5% of Helioguard®365 reduced DNA damage of IMR- 90 human fibroblasts following exposure to UV-A radiation. A study conducted in vivo on ten human subjects showed that Helioguard®365 (2% concentration) boosted the SPF value of sunscreen from 7.2 to 8.2 [129]. Likewise, twenty women volunteers applied a 5% concentration of Helioguard®365 twice a day. After four weeks this treatment prevented the appearance of lines and wrinkles on the skin and improved firmness and smoothness by 10% and 12%, respectively. This commercial product has broad stability such that it can be stable for 3 months at temperatures ranging from 4 to 37 C [66,128]. In addition, Torres et al. [86] determined the SPF value of a formulation of collemin A and olive oil, in a ratio of 1:10, on the inside forearm of a volunteer at a concentration of 6 gcm?2. Fifteen minutes after application, the treated area was exposed to four MEDs (360 mJ cm?2) of UV-B radiation and 24 h later erythema was observed. They observed that in comparison to the positive control, i.e., octinoxate commercial sunscreen, this formulation was equally effective. However, the reliability of this study is low because only a single volunteer was involved. de la Coba et al. [125] studied the SPF value of a sunscreen formulation containing porphyra-334 (+shinorine) as a UV-A filter, and mycosporine-serinol as a UV-B filter in the ratio of 4.1:2.9%, respectively. They observed that separately these MAAs had SPF values between 4 and 6. However, in combination, the value increased to 8.37 2.12, which is quite similar to the value of 9.54 1.53 for the reference sunscreen formulation of avobenzone (UV-A) and octinoxate (UV-B) in a ratio of 4.5:2.6%, respectively. This study was performed in vitro [125]. Likewise, the SPF values of the algal extracts (containing palythine, asterina-330, shinorine, porphyra-334 and palythinol) of H. cornea and G. longissima showed a gradual increase with increasing concentration of extract. The highest values of SPF were recorded at 13.9 mg DW of algae per cm?2 which was 7.5 for G. longissima and 4.8 for H. cornea [123]. Helionori® is another commercially active natural sunscreen product that includes palythine, porphyra-334 and shinorine and was extracted from the red algae P. umbilicalis. This product exhibited UV-A protective effects on human fibroblasts and keratinocytes cell lines, and, like 2% Helionori®, increased cell viability by up to 57 and 135% in cultures of human keratinocytes and fibroblast cell lines, respectively. This product has excellent stability against exposure to light and temperature and it can also provide a maximum level of protection to DNA by preserving membrane lipids [66,82,130]. By making comparisons over the past few decades, it is clear that the use of naturally synthesized products has significantly augmented and replaced chemical-based products. Along with other bio-based products, MAAs have also gained the attention of several researchers and industries. The application of MAA-based sunscreens may be an efficient and safer alternative for health products and cosmetics. As discussed in the previous paragraph, there are very few commercial MAA-based sunscreens on the market, so thereis still a long way to go to gain acceptance of naturally derived sunscreens such as MAAs.
5. Conclusions
A rapid increase in skin damage in humans due to UV radiation has been reported over the past few decades, which has led to the use of many chemical/physical UV filters in order to protect skin against damage. However, a wide range of chemicals that are used to treat skin damage also have harmful effects on human health, the environment and damage to aquatic life, eventually disturbing the whole ecosystem through their bioaccumulation. In this review, we considered the use of MAAs as a natural sunscreen against skin damage, and which can be used as an adequate substitute for damaging and harmful chemicals. MAAs are known to be a functionally very diverse group of natural compounds that effectively absorb UV rays. Apart from functioning as a photo protectant, MAAs also act as anti-photoaging compounds, cell proliferation activators, anti-inflammatory or anticancer agents, and skin cell renewal stimulators. MAAs are now attracting commercial attention since they can provide a wide range of protection against UV rays. They conjugate with biopolymers or nanoparticles, eventually increasing their stability and effectiveness. Despite having extensive literature on the extraction and characterization of MAAs from their sources, the critical mechanisms involved in their protection against UVR has yet to be clearly addressed and is a topic for further research. Ultimately, they may become commercially available as a personalized natural sunscreen.
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