Recombinant antigens are antigenic proteins or protein-derived constructs produced through expression of engineered genetic sequences. Their molecular identity includes the encoded amino-acid sequence and any structural or chemical features introduced during biosynthesis. Glycosylation can contribute directly to recognized epitopes or influence the folding and accessibility of antigenic surfaces [1]. Recombinant expression can generate a selected antigenic protein as part of a larger fusion polypeptide [2]. The spatial arrangement of exposed residues establishes conformational epitopes recognized by antibodies, making folding and structural integrity integral properties of the expressed antigen [3]. Genetic fusion can also join an antigenic sequence to another protein domain within a single translation product [4].
- recombinant antigens
- protein expression
- antigenic determinants
- protein conformation
Molecular Biology Techniques and Applications·Molecular Biology·Biochemistry, Genetics and Molecular Biology·Life Sciences
References
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