A rate limiting enzyme is an enzyme whose activity substantially constrains flux through a metabolic pathway under specified conditions. Metabolic control analysis quantifies this relationship by measuring how a change in enzyme activity changes the flux of the connected system [1]. Control can be distributed among several reactions and can shift when the metabolic state changes [2]. Regulatory circuits that coordinate expression with intracellular metabolites can alter the balance between precursor supply and downstream conversion [3]. In particular settings, regulation of one biosynthetic enzyme can exert strong control over production of a required metabolite pool [4]. The designation therefore requires evidence about the whole pathway under the conditions examined. Enzyme abundance, kinetic parameters, and reaction reversibility alone do not establish its quantitative contribution to pathway control [5].
- metabolic control
- pathway flux
- enzyme capacity
- flux control coefficient
Enzyme function and inhibition·Molecular Biology·Biochemistry, Genetics and Molecular Biology·Life Sciences
References
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