Practical Methods for Liver Cell Biology: Comparison
Please note this is a comparison between Version 3 by Camila Xu and Version 2 by Sandra Torres Núñez.

Molecular and cellular research modalities for the study of liver pathologies have been tremendously improved over the recent decades. Advanced technologies offer novel opportunities to establish cell isolation techniques with excellent purity, paving the path for 2D and 3D microscopy and high-throughput assays (e.g., bulk or single-cell RNA sequencing). The use of stem cell and organoid research will help to decipher the pathophysiology of liver diseases and the interaction between various parenchymal and non-parenchymal liver cells. Furthermore, sophisticated animal models of liver disease allow for the in vivo assessment of fibrogenesis, portal hypertension and hepatocellular carcinoma (HCC) and for the preclinical testing of therapeutic strategies. The purpose of this review is to portray in detail novel in vitro and in vivo methods for the study of liver cell biology that had been presented at the workshop of the 8th meeting of the European Club for Liver Cell Biology (ECLCB-8) in October of 2018 in Bonn, Germany. 

  • hepatic stellate cells
  • hepatocellular cancer
  • fibrogenesis
  • steatosis
  • in vitro models
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References

  1. Kaori Endo-Umeda; Makoto Makishima; Liver X Receptors Regulate Cholesterol Metabolism and Immunity in Hepatic Nonparenchymal Cells.. International Journal of Molecular Sciences 2019, 20, 5045, 10.3390/ijms20205045.
  2. G Pott; J Rauterberg; B Voss; U Gerlach; Connective tissue components of the normal and fibrotic human liver. II. Clinical aspects.. null 1982, 60, , null.
  3. Kyo-Sang Yoo; Woo Taek Lim; Ho Soon Choi; Biology of Cholangiocytes: From Bench to Bedside. Gut and Liver 2016, 10, 687-698, 10.5009/gnl16033.
  4. Oliver Krenkel; Jana Hundertmark; Ali T Abdallah; Marlene Kohlhepp; Tobias Puengel; Tilmann Roth; Diogo Philippini Pontual Branco; Jana C Mossanen; Tom Luedde; Christian Trautwein; Ivan G Costa; Frank Tacke; Myeloid cells in liver and bone marrow acquire a functionally distinct inflammatory phenotype during obesity-related steatohepatitis. Gut 2019, 69, 551-563, 10.1136/gutjnl-2019-318382.
  5. Robert Huebert; Sampaziotis F; Justin Aw; Tysoe Oc; Sawiak S; Godfrey Em; Upponi Ss; Gieseck Rl; De Brito Mc; Berntsen Nl; Gómez-Vázquez Mj; Ortmann D; Yiangou L; Ross A; Bargehr J; Bertero A; Zonneveld McF; Pedersen Mt; Pawlowski M; Valestrand L; Madrigal P; Georgakopoulos N; Pirmadjid N; Skeldon Gm; Casey J; Shu W; Materek Pm; Snijders Ke; Brown Se; Rimland Ca; Simonic I; Davies Se; Jensen Kb; Zilbauer M; Gelson Wth; Alexander Gj; Sinha S; Hannan Nrf; Wynn Ta; Karlsen Th; Melum E; Markaki Ae; Saeb-Parsy K; Vallier L; Faculty of 1000 evaluation for Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids.. F1000 - Post-publication peer review of the biomedical literature 2017, 23, , 10.3410/f.727772304.793536483.
  6. H. Tsukamoto; R. D. Reidelberger; S. W. French; C. Largman; Long-term cannulation model for blood sampling and intragastric infusion in the rat. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 1984, 247, R595-R599, 10.1152/ajpregu.1984.247.3.r595.
  7. Maximilian J Brol; Felicitas Rösch; Robert Schierwagen; Fernando Magdaleno; Frank Erhard Uschner; Steffen Manekeller; Alexander Queck; Katharina Schwarzkopf; Margarete Odenthal; Uta Drebber; Maja Thiele; Philipp Lingohr; Andreas Plamper; Glen Kristiansen; Sophie Lotersztajn; Aleksander Krag; Sabine Klein; Karl P. Rheinwalt; Jonel Trebicka; Combination of CCl4 with alcoholic and metabolic injuries mimics human liver fibrosis.. American Journal of Physiology-Gastrointestinal and Liver Physiology 2019, 317, G182-G194, 10.1152/ajpgi.00361.2018.
  8. Matthias Bartneck; Peter L. Schrammen; Diana Möckel; Olivier Govaere; Anke Liepelt; Oliver Krenkel; Can Ergen; Misti Vanette McCain; Dirk Eulberg; Tom Luedde; Christian Trautwein; Fabian Kiessling; Helen Reeves; Twan Lammers; Frank Tacke; The CCR2+ Macrophage Subset Promotes Pathogenic Angiogenesis for Tumor Vascularization in Fibrotic Livers. Cellular and Molecular Gastroenterology and Hepatology 2019, 7, 371-390, 10.1016/j.jcmgh.2018.10.007.
  9. Takuma Tsuchida; Youngmin A. Lee; Naoto Fujiwara; Maria Ybanez; Brittany Allen; Sebastiao Martins; M. Isabel Fiel; Nicolas Goossens; Hsin-I. Chou; Yujin Hoshida; Scott L. Friedman; Corrigendum to "A simple diet- and chemical-induced murine NASH model with rapid progression of steatohepatitis, fibrosis and liver cancer" [J Hepatol 69 (2018) 385-395].. Journal of Hepatology 2018, 69, 988, 10.1016/j.jhep.2018.07.010.
  10. Albert Geerts; Toshiro Niki; Karine Hellemans; Dirk De Craemer; Kit Van Den Berg; Jean-Marc Lazou; Geert Stange; Marnix Van De Winkel; Pieter De Bleser; Purification of rat hepatic stellate cells by side scatter-activated cell sorting. Hepatology 1998, 27, 590-598, 10.1002/hep.510270238.
  11. Sabine Weiskirchen; Carmen G. Tag; Sibille Sauer-Lehnen; Frank Tacke; Ralf Weiskirchen; Isolation and Culture of Primary Murine Hepatic Stellate Cells. Advanced Structural Safety Studies 2017, 1627, 165-191, 10.1007/978-1-4939-7113-8_11.
  12. Frank Tacke; Ralf Weiskirchen; Update on hepatic stellate cells: pathogenic role in liver fibrosis and novel isolation techniques. Expert Review of Gastroenterology & Hepatology 2012, 6, 67-80, 10.1586/egh.11.92.
  13. Jana C. Mossanen; Oliver Krenkel; Can Ergen; Olivier Govaere; Anke Liepelt; Tobias Puengel; Felix Heymann; Sandra Kalthoff; Eric Lefebvre; Dirk Eulberg; Tom Luedde; Gernot Marx; Christian P. Strassburg; Tania Roskams; Christian Trautwein; Frank Tacke; Chemokine (C-C motif) receptor 2-positive monocytes aggravate the early phase of acetaminophen-induced acute liver injury. Hepatology 2016, 64, 1667-1682, 10.1002/hep.28682.
  14. A. Mennone; Domenico Alvaro; W. Cho; J. L. Boyer; Isolation of small polarized bile duct units.. Proceedings of the National Academy of Sciences 1995, 92, 6527-6531, 10.1073/pnas.92.14.6527.
  15. Li Yang; Ronald A. Faris; Uglas C. Hixson; Characterization of a mature bile duct antigen expressed on a subpopulation of biliary ductular cells but absent from oval cells. Hepatology 1993, 18, 357-366, 10.1002/hep.1840180220.
  16. Mühlbauer, M.; Weiss, T.S.; Thasler, W.E.; Gelbmann, C.M.; Schnabl, B.; Schölmerich, J.; Hellerbrand, C. LPS-mediated NFkappaB activation varies between activated human hepatic stellate cells from different donors. Biochem. Biophys. Res. Commun. 2004, 325, 191–197. [Google Scholar] [CrossRef]
  17. Claus Hellerbrand; Hepatic stellate cells—the pericytes in the liver. Pflügers Archiv - European Journal of Physiology 2013, 465, 775-778, 10.1007/s00424-012-1209-5.
  18. Tatjana Seitz; Kim Freese; Peter Dietrich; Wolfgang Erwin Thasler; Anja Bosserhoff; Claus Hellerbrand; Fibroblast Growth Factor 9 is expressed by activated hepatic stellate cells and promotes progression of hepatocellular carcinoma. Scientific Reports 2020, 10, 1-9, 10.1038/s41598-020-61510-4.
  19. Theresa Meyer; Andreas Koch; Eva-Vanessa Ebert; Barbara Czech; Martina Mueller; Anja Bosserhoff; Sven Arke Lang; Claus Hellerbrand; Effect of melanoma cells on proliferation and migration of activated hepatic stellate cells in vitro. Pathology - Research and Practice 2017, 213, 400-404, 10.1016/j.prp.2016.12.014.
  20. Hella Wobser; Christoph Dorn; Thomas S. Weiss; Thomas Amann; Cornelius Bollheimer; Roland Büttner; Jürgen Schölmerich; Claus Hellerbrand; Roland B; [Uuml]; Lipid accumulation in hepatocytes induces fibrogenic activation of hepatic stellate cells. Cell Research 2009, 19, 996-1005, 10.1038/cr.2009.73.
  21. Oliver Krenkel; Tobias Puengel; Olivier Govaere; Ali T. Abdallah; Jana C. Mossanen; Marlene Kohlhepp; Anke Liepelt; Eric Lefebvre; Tom Luedde; Claus Hellerbrand; Ralf Weiskirchen; Thomas Longerich; Ivan G. Costa; Quentin M. Anstee; Christian Trautwein; Frank Tacke; Therapeutic inhibition of inflammatory monocyte recruitment reduces steatohepatitis and liver fibrosis.. Hepatology 2018, 67, 1270-1283, 10.1002/hep.29544.
  22. Charlotte L. Scott; Wouter T’Jonck; Liesbet Martens; Helena Todorov; Dorine Sichien; Bieke Soen; Johnny Bonnardel; Sofie De Prijck; Niels Vandamme; Robrecht Cannoodt; Wouter Saelens; Bavo Vanneste; Wendy Toussaint; Pieter De Bleser; Nozomi Takahashi; Peter Vandenabeele; Sandrine Henri; Clare Pridans; David A. Hume; Bart N. Lambrecht; Patrick De Baetselier; Simon W.F. Milling; Jo A. Van Ginderachter; Bernard Malissen; Geert Berx; Alain Beschin; Yvan Saeys; Martin Guilliams; The Transcription Factor ZEB2 Is Required to Maintain the Tissue-Specific Identities of Macrophages.. Immunity 2018, 49, 312-325.e5, 10.1016/j.immuni.2018.07.004.
  23. Sonya A. MacParland; Jeff C. Liu; Xue-Zhong Ma; Brendan Innes; Agata M. Bartczak; Blair K. Gage; Justin Manuel; Nicholas Khuu; Juan Echeverri; Ivan Linares; Rahul Gupta; Michael Cheng; Lewis Y. Liu; Damra Camat; Sai W. Chung; Rebecca K. Seliga; Zigong Shao; Elizabeth Lee; Shinichiro Ogawa; Mina Ogawa; Michael D. Wilson; Jason E. Fish; Markus Selzner; Anand Ghanekar; D. R. Grant; Paul Greig; Gonzalo Sapisochin; Nazia Selzner; Neil Winegarden; Oyedele Adeyi; Gordon Keller; Gary Bader; Ian D. McGilvray; Single cell RNA sequencing of human liver reveals distinct intrahepatic macrophage populations. Nature Communications 2018, 9, 4383, 10.1038/s41467-018-06318-7.
  24. Neta Lavon; Ofra Yanuka; Nissim Benvenisty; Differentiation and isolation of hepatic-like cells from human embryonic stem cells. Differentiation 2004, 72, 230-238, 10.1111/j.1432-0436.2004.07205002.x.
  25. Robert E. Schwartz; Jonathan L. Linehan; Matthew S. Painschab; Wei-Shou Hu; Catherine M. Verfaillie; Dan S. Kaufman; Defined Conditions for Development of Functional Hepatic Cells from Human Embryonic Stem Cells. Stem Cells and Development 2005, 14, 643-655, 10.1089/scd.2005.14.643.
  26. Arefeh Ghodsizadeh; Adeleh Taei; Mehdi Totonchi; Ali Seifinejad; Hamid Gourabi; Behshad Pournasr; Nasser Aghdami; Reza Malekzadeh; Navid Almadani; Ghasem Hosseini Salekdeh; Hossein Baharvand; Generation of Liver Disease-Specific Induced Pluripotent Stem Cells Along with Efficient Differentiation to Functional Hepatocyte-Like Cells. Stem Cell Reviews and Reports 2010, 6, 622-632, 10.1007/s12015-010-9189-3.
  27. Romina Fiorotto; Mariangela Amenduni; Valeria Mariotti; Luca Fabris; Carlo Spirli; Mario Strazzabosco; Liver diseases in the dish: iPSC and organoids as a new approach to modeling liver diseases.. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2018, 1865, 920-928, 10.1016/j.bbadis.2018.08.038.
  28. Patricio Godoy; Nicola J. Hewitt; Ute Albrecht; Melvin E. Andersen; Nariman Ansari; Sudin Bhattacharya; Johannes Georg Bode; Jennifer Bolleyn; Christoph Borner; Jan Böttger; Albert Braeuning; Robert A. Budinsky; Britta Burkhardt; Neil R. Cameron; Giovanni Camussi; Chong-Su Cho; Yun-Jaie Choi; J. Craig Rowlands; Uta Dahmen; Georg Damm; Olaf Dirsch; M. Teresa Donato; Jian Dong; Steven Dooley; Dirk Drasdo; Rowena Eakins; Karine Sá Ferreira; Valentina Fonsato; Joanna Fraczek; Rolf Gebhardt; Andrew Gibson; Matthias Glanemann; Chris E. P. Goldring; María José Gomez-Lechon; Geny M. M. Groothuis; Lena Gustavsson; Christelle Guyot; David Hallifax; Seddik Hammad; Adam Simon Hayward; Dieter Häussinger; Claus Hellerbrand; Philip Hewitt; Stefan Hoehme; Hermann-Georg Holzhütter; J. Brian Houston; Jens Hrach; Kiyomi Ito; Hartmut Jaeschke; Verena Keitel; Jens M. Kelm; B. Kevin Park; Claus Kordes; Gerd A. Kullak-Ublick; Edward Lecluyse; Peng Lu; Jennifer Luebke-Wheeler; Anna Lutz; Daniel J. Maltman; Madlen Matz-Soja; Patrick McMullen; Irmgard Merfort; Simon Messner; Christoph Meyer; Jessica Mwinyi; Dean J. Naisbitt; Andreas K. Nussler; Peter Olinga; Francesco Pampaloni; Jingbo Pi; Linda Pluta; Stefan A. Przyborski; Anup Ramachandran; Vera Rogiers; Cliff Rowe; Celine Schelcher; Kathrin Schmich; Michael Schwarz; Bijay Singh; Ernst H. K. Stelzer; Bruno Stieger; Regina Stöber; Yuichi Sugiyama; Ciro Tetta; Wolfgang E Thasler; Tamara Vanhaecke; Mathieu Vinken; Thomas S. Weiss; Agata Widera; Courtney G. Woods; Jinghai James Xu; Kathy M. Yarborough; Jan G. Hengstler; Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME. Archives of Toxicology 2013, 87, 1315-530, 10.1007/s00204-013-1078-5.
  29. Marta Ramirez; Angiogenesis in Liver Disease Research Group; Mercedes Fernandez; 08036 Barcelona Rossello 149-153; Pathological Angiogenesis: The New Culprit behind Chronic Liver Disease. OBM Hepatology and Gastroenterology 2019, 3, 1-1, 10.21926/obm.hg.1903030.
  30. Ester García-Pras; Javier Gallego; Laura Coch; Marc Mejias; Gonzalo Fernandez-Miranda; Ricardo Pardal; Jaime Bosch; Raúl Méndez; Mercedes Fernandez; Role and therapeutic potential of vascular stem/progenitor cells in pathological neovascularisation during chronic portal hypertension. Gut 2016, 66, 1306-1320, 10.1136/gutjnl-2015-311157.
  31. Feifei Guo; Kang Zheng; Raquel Benedé-Ubieto; Francisco Javier Cubero; Yulia A. Nevzorova; The Lieber-DeCarli Diet-A Flagship Model for Experimental Alcoholic Liver Disease. Alcoholism: Clinical and Experimental Research 2018, 42, 1828-1840, 10.1111/acer.13840.
  32. Liquid Diet Technique of ethanol administration: 1989 update. Alcohol Alcohol. 1989, 24, 197–211.
  33. Bertola, A.; Mathews, S.; Ki, S.H.; Wang, H.; Gao, B. Mouse model of chronic and binge ethanol feeding (the NIAAA model). Nat. Protoc. 2013, 8, 627–637. [Google Scholar] [CrossRef] [PubMed]
  34. Sameek Roychowdhury; Dian J. Chiang; Megan R. McMullen; Laura E. Nagy; Moderate, chronic ethanol feeding exacerbates carbon tetrachloride-induced hepatic fibrosis via hepatocyte-specific hypoxia-inducible factor 1α. Pharmacology Research & Perspectives 2014, 2, e00061, 10.1002/prp2.61.
  35. Robert Schierwagen; Lara Maybüchen; Sebastian Zimmer; Kanishka Hittatiya; Christer Bäck; Sabine Klein; Frank E. Uschner; Winfried Reul; Peter Boor; Georg Nickenig; Christian P. Strassburg; Christian Trautwein; Jogchum Plat; Dieter Lütjohann; Tilman Sauerbruch; Frank Tacke; Jonel Trebicka; Seven weeks of Western diet in apolipoprotein-E-deficient mice induce metabolic syndrome and non-alcoholic steatohepatitis with liver fibrosis. Scientific Reports 2015, 5, 12931, 10.1038/srep12931.
  36. Priya Handa; Vicki Morgan-Stevenson; Bryan D. Maliken; James E. Nelson; Shenna Washington; Mark Westerman; Matthew M. Yeh; Kris V Kowdley; Iron overload results in hepatic oxidative stress, immune cell activation, and hepatocellular ballooning injury, leading to nonalcoholic steatohepatitis in genetically obese mice. American Journal of Physiology-Gastrointestinal and Liver Physiology 2016, 310, G117-G127, 10.1152/ajpgi.00246.2015.
  37. Montserrat Marí; Francisco Caballero; Anna Colell; Albert Morales; Juan Caballería; Ana Fernandez; Carlos Enrich; José C. Fernándezcheca; Carmen García-Ruiz; Mitochondrial free cholesterol loading sensitizes to TNF- and Fas-mediated steatohepatitis. Cell Metabolism 2006, 4, 185-198, 10.1016/j.cmet.2006.07.006.
  38. Frank Tacke; Targeting hepatic macrophages to treat liver diseases. Journal of Hepatology 2017, 66, 1300-1312, 10.1016/j.jhep.2017.02.026.
  39. Howard Spiro; Animal models of portal hypertension. Gastroenterology 1989, 96, 1223-1224, 10.1016/0016-5085(89)91656-9.
  40. Christer Baeck; Alexander Wehr; Karlin Raja Karlmark; Felix Heymann; Mihael Vucur; Nikolaus Gassler; Sebastian Huss; Sven Klussmann; Dirk Eulberg; Tom Luedde; Christian Trautwein; Frank Tacke; Pharmacological inhibition of the chemokine CCL2 (MCP-1) diminishes liver macrophage infiltration and steatohepatitis in chronic hepatic injury. Gut 2011, 61, 416-426, 10.1136/gutjnl-2011-300304.
  41. Claudia Von Montfort; Nuria Matias; Ana Fernandez; Raquel Fucho; Laura Conde De La Rosa; María Luz Martínez-Chantar; José M Mato; Keigo Machida; Hidekazu Tsukamoto; Michael P. Murphy; Abdellah Mansouri; Neil Kaplowitz; Carmen García-Ruiz; José C. Fernándezcheca; Mitochondrial GSH determines the toxic or therapeutic potential of superoxide scavenging in steatohepatitis.. Journal of Hepatology 2012, 57, 852-9, 10.1016/j.jhep.2012.05.024.
  42. Mikhaïl A. Van Herck; Luisa Vonghia; S. Francque; Animal Models of Nonalcoholic Fatty Liver Disease—A Starter’s Guide. Nutrients 2017, 9, 1072, 10.3390/nu9101072.
  43. Longato, L. Non-alcoholic fatty liver disease (NAFLD): A tale of fat and sugar? Fibrogenes. Tissue Repair 2013, 6, 14. [Google Scholar] [CrossRef] [PubMed]
  44. Per Lindström; The Physiology of Obese-Hyperglycemic Mice [ob/ob Mice]. The Scientific World Journal 2007, 7, 666-685, 10.1100/tsw.2007.117.
  45. Christian Liedtke; Tom Luedde; Tilman Sauerbruch; D. Scholten; K.L. Streetz; Frank Tacke; René H Tolba; C. Trautwein; Jonel Trebicka; Ralf Weiskirchen; Experimental liver fibrosis research: update on animal models, legal issues and translational aspects. Fibrogenesis & Tissue Repair 2013, 6, 19-19, 10.1186/1755-1536-6-19.
  46. Tom Luedde; Naiara Beraza; Vasileios Kotsikoris; Geert Van Loo; Arianna Nenci; Rita De Vos; Tania Roskams; Christian Trautwein; Manolis Pasparakis; Deletion of NEMO/IKKγ in Liver Parenchymal Cells Causes Steatohepatitis and Hepatocellular Carcinoma. Cancer Cell 2007, 11, 119-132, 10.1016/j.ccr.2006.12.016.
  47. J.J.M. Smit; A.H. Schinkel; R.P.J.Oude Elferink; A.K. Groen; E. Wagenaar; L. Van Deemter; C.A.A.M. Mol; R. Ottenhoff; N.M.T. Van Der Lugt; M.A. Van Roon; M.A. Van Der Valk; G.J.A. Offerhaus; A.J.M. Berns; P. Borst; Homozygous disruption of the murine MDR2 P-glycoprotein gene leads to a complete absence of phospholipid from bile and to liver disease. Cell 1993, 75, 451-462, 10.1016/0092-8674(93)90380-9.
  48. Tolba, R.; Kraus, T.; Liedtke, C.; Schwarz, M.; Weiskirchen, R. Diethylnitrosamine (DEN)-induced carcinogenic liver injury in mice. Lab. Anim. 2015, 49, 59–69. [Google Scholar] [CrossRef]
  49. Uehara, T.; Pogribny, I.P.; Rusyn, I. The DEN and CCl4 -Induced Mouse Model of Fibrosis and Inflammation-Associated Hepatocellular Carcinoma. Curr. Protoc. Pharmacol. 2014, 66, 1–10. [Google Scholar] [CrossRef]
  50. S Klein; C Hinüber; K Hittatiya; R Schierwagen; Fe Uschner; Cp Strassburg; Hp Fischer; U Spengler; J Trebicka; Novel rat model of repetitive portal venous embolization mimicking human non-cirrhotic idiopathic portal hypertension. Zeitschrift für Gastroenterologie 2016, 54, 1343-1404, 10.1055/s-0036-1597369.
  51. Philipp Königshofer; Ksenia Brusilovskaya; Philipp Schwabl; Bruno Podesser; Michael Trauner; Thomas Reiberger; Invasive Hemodynamic Characterization of the Portal-hypertensive Syndrome in Cirrhotic Rats. Journal of Visualized Experiments 2018, null, , 10.3791/57261.
  52. Sabine Klein; Johanna Rick; Jennifer Lehmann; Robert Schierwagen; Irela Gretchen Schierwagen; Len Verbeke; Kanishka Hittatiya; Frank Erhard Uschner; Steffen Manekeller; Christian P Strassburg; Kay-Uwe Wagner; Peter P. Sayeski; Dominik Wolf; Wim Laleman; Tilman Sauerbruch; Jonel Trebicka; Janus-kinase-2 relates directly to portal hypertension and to complications in rodent and human cirrhosis. Gut 2015, 66, 145-155, 10.1136/gutjnl-2015-309600.
  53. Marc Mejias; Carolina Tiani; Rosa Miquel; Mercedes Fernandez; Ester García-Pras; Jaime Bosch; Beneficial effects of sorafenib on splanchnic, intrahepatic, and portocollateral circulations in portal hypertensive and cirrhotic rats†. Hepatology 2008, 49, 1245-1256, 10.1002/hep.22758.
  54. Vittorio Calderone; Javier Gallego; Gonzalo Fernandez-Miranda; Ester García-Pras; Carlos Maillo; Annalisa Berzigotti; Marc Mejias; Felice-Alessio Bava; Ana Angulo-Urarte; Mariona Graupera; Pilar Navarro; Jaime Bosch; Mercedes Fernandez; Raúl Méndez; Sequential Functions of CPEB1 and CPEB4 Regulate Pathologic Expression of Vascular Endothelial Growth Factor and Angiogenesis in Chronic Liver Disease. Gastroenterology 2016, 150, 982-997.e30, 10.1053/j.gastro.2015.11.038.
  55. Carlos Maillo; Judit Martín; David Sebastián; María Isabel Hernández-Alvarez; Mar García-Rocha; Oscar Reina; Antonio Zorzano; Mercedes Fernandez; Raúl Méndez; Circadian- and UPR-dependent control of CPEB4 mediates a translational response to counteract hepatic steatosis under ER stress. Nature 2017, 19, 94-105, 10.1038/ncb3461.
  56. Laura Coch; Marc Mejias; Annalisa Berzigotti; Javier Gallego; Jaime Bosch; Raúl Méndez; Mercedes Fernandez; Ester García-Pras; Disruption of negative feedback loop between vasohibin-1 and vascular endothelial growth factor decreases portal pressure, angiogenesis, and fibrosis in cirrhotic rats. Hepatology 2014, 60, 633-647, 10.1002/hep.26995.
  57. A Blouin; R P Bolender; E R Weibel; Distribution of organelles and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A stereological study. Journal of Cell Biology 1977, 72, 441-455, null.
  58. Ryan J. Schulze; Micah B. Schott; Carol A. Casey; Pamela L. Tuma; Mark A. McNiven; The cell biology of the hepatocyte: A membrane trafficking machine. Journal of Cell Biology 2019, 218, 2096-2112, 10.1083/jcb.201903090.
  59. A. L. Dounce; THE PREPARATION OF ISOLATED CELL NUCLEI OF RAT LIVER. Science 1944, 99, 512-513, 10.1126/science.99.2582.512.
  60. Victoria Kegel; Daniela Deharde; Elisa Pfeiffer; Katrin Zeilinger; Daniel Seehofer; Georg Damm; Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells. Journal of Visualized Experiments 2016, null, e53069-e53069, 10.3791/53069.
  61. M. Teresa Donato; Miguel Bolonio; Estefanía Cabezas; María Pelechá; Eugenia Pareja; Anna Domènech; José V. Castell; M. José Gómez-Lechón; Laia Tolosa; Improved in vivo efficacy of clinical-grade cryopreserved human hepatocytes in mice with acute liver failure. Cytotherapy 2020, 22, 114-121, 10.1016/j.jcyt.2019.12.005.
  62. Behshad Pournasr; Stephen Duncan; Modeling Inborn Errors of Hepatic Metabolism Using Induced Pluripotent Stem Cells.. Arteriosclerosis, Thrombosis, and Vascular Biology 2017, 37, 1994-1999, 10.1161/ATVBAHA.117.309199.
  63. James L. Corbett; Stephen Duncan; iPSC-Derived Hepatocytes as a Platform for Disease Modeling and Drug Discovery.. Frontiers in Medicine 2019, 6, 265, 10.3389/fmed.2019.00265.
  64. Andrés J M Ferreri; Gerald Illerhaus; Emanuele Zucca; Franco Cavalli; International Extranodal Lymphoma Study group; on behalf of the International Extranodal Lymphoma Study Group; Flows and flaws in primary central nervous system lymphoma.. Nature Reviews Clinical Oncology 2010, 7, 1-2, 10.1038/nrclinonc.2010.9-c1.
  65. Hans-Theo Schon; Matthias Bartneck; Erawan Borkham-Kamphorst; Jacob Nattermann; Twan Lammers; Frank Tacke; Ralf Weiskirchen; Pharmacological Intervention in Hepatic Stellate Cell Activation and Hepatic Fibrosis. Frontiers in Pharmacology 2016, 7, 536, 10.3389/fphar.2016.00033.
  66. H.F.J. Hendriks; W.A.M.M. Verhoofstad; A. Brouwer; A.M. De Leeuw; D.L. Knook; Perisinusoidal fat-storing cells are the main vitamin A storage sites in rat liver. Experimental Cell Research 1985, 160, 138-149, 10.1016/0014-4827(85)90243-5.
  67. Samuele De Minicis; Ekihiro Seki; Hiroshi Uchinami; Johannes Kluwe; Yonghui Zhang; David A. Brenner; Robert F. Schwabe; Gene Expression Profiles During Hepatic Stellate Cell Activation in Culture and In Vivo. Gastroenterology 2007, 132, 1937-1946, 10.1053/j.gastro.2007.02.033.
  68. Ryan E. Feaver; Banumathi K. Cole; Mark J. Lawson; Stephen A. Hoang; Svetlana Marukian; Brett R. Blackman; Robert A. Figler; Arun J. Sanyal; Brian R. Wamhoff; Ajit Dash; Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis.. JCI Insight 2016, 1, e90954, 10.1172/jci.insight.90954.
  69. Marcus Mühlbauer; Anja Bosserhoff; Arndt Hartmann; Wolfgang E Thasler; Thomas S. Weiss; Hans Herfarth; Guntram Lock; Jürgen Schölmerich; Claus Hellerbrand; A novel MCP-1 gene polymorphism is associated with hepatic MCP-1 expression and severity of HCV-related liver disease.. Gastroenterology 2003, 125, 1085-1093, 10.1016/s0016-5085(03)01213-7.
  70. Julia C. Engelmann; Thomas Amann; Birgitta Ott-Rötzer; Margit Nützel; Yvonne Reinders; Jörg Reinders; Wolfgang E. Thasler; Theresa Kristl; Andreas Teufel; Christian G Huber; Peter J. Oefner; Rainer Spang; Claus Hellerbrand; Causal Modeling of Cancer-Stromal Communication Identifies PAPPA as a Novel Stroma-Secreted Factor Activating NFκB Signaling in Hepatocellular Carcinoma. PLOS Computational Biology 2015, 11, e1004293, 10.1371/journal.pcbi.1004293.
  71. Oliver Krenkel; Frank Tacke; Liver macrophages in tissue homeostasis and disease. Nature Reviews Immunology 2017, 17, 306-321, 10.1038/nri.2017.11.
  72. Prakash Ramachandran; R. Dobie; J. R. Wilson-Kanamori; E. F. Dora; B. E. P. Henderson; N. T. Luu; J. R. Portman; K. P. Matchett; M. Brice; J. A. Marwick; R. S. Taylor; M. Efremova; R. Vento-Tormo; N. O. Carragher; T. J. Kendall; J. A. Fallowfield; E. M. Harrison; D. J. Mole; S. J. Wigmore; P. N. Newsome; C. J. Weston; J. P. Iredale; F. Tacke; J. W. Pollard; C. P. Ponting; J. C. Marioni; S. A. Teichmann; N. C. Henderson; Resolving the fibrotic niche of human liver cirrhosis at single-cell level. Nature 2019, 575, 512-518, 10.1038/s41586-019-1631-3.
  73. Matthias Bartneck; Klaudia Theresa Warzecha; Carmen Gabriele Tag; Sibille Sauer-Lehnen; Felix Heymann; Christian Trautwein; Ralf Weiskirchen; Frank Tacke; Isolation and Time Lapse Microscopy of Highly Pure Hepatic Stellate Cells. Analytical Cellular Pathology 2015, 2015, 1-13, 10.1155/2015/417023.
  74. Johnny Bonnardel; Wouter T’Jonck; Djoere Gaublomme; Robin Browaeys; Charlotte L. Scott; Liesbet Martens; Bavo Vanneste; Sofie De Prijck; Sergei A. Nedospasov; Anna Kremer; Evelien Van Hamme; Peter Borghgraef; Wendy Toussaint; Pieter De Bleser; Inge Mannaerts; Alain Beschin; Leo A. Van Grunsven; Bart N. Lambrecht; Tom Taghon; Saskia Lippens; Dirk Elewaut; Yvan Saeys; Martin Guilliams; Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche.. Immunity 2019, 51, 638-654.e9, 10.1016/j.immuni.2019.08.017.
  75. Maureen E. Taylor; Tom Snelling; David F Smith; Kurt Drickamer; Absence of a human ortholog of rodent Kupffer cell galactose-binding receptor encoded by the CLEC4f gene.. Glycobiology 2019, 29, 332-345, 10.1093/glycob/cwy113.
  76. Mario Strazzabosco; Luca Fabris; Functional anatomy of normal bile ducts.. The Anatomical Record 2008, 291, 653-60, 10.1002/ar.20664.
  77. Luca Fabris; Carlo Spirli; Massimiliano Cadamuro; Romina Fiorotto; Mario Strazzabosco; Emerging concepts in biliary repair and fibrosis.. American Journal of Physiology-Gastrointestinal and Liver Physiology 2017, 313, G102-G116, 10.1152/ajpgi.00452.2016.
  78. Carlo Spirli; Luca Fabris; Elena Duner; Romina Fiorotto; Giorgio Ballardini; Tania Roskams; Nicholas F. LaRusso; Aurelio Sonzogni; Lajos Okolicsanyi; Mario Strazzabosco; Cytokine-stimulated nitric oxide production inhibits adenylyl cyclase and cAMP-dependent secretion in cholangiocytes. Gastroenterology 2003, 124, 737-753, 10.1053/gast.2003.50100.
  79. Carlo Spirlì; Romina Fiorotto; Lei Song; Joseph Santos-Sacchi; Lajos Okolicsanyi; Sara Masier; Loretta Rocchi; Mariapia Vairetti; Marina De Bernard; Saida Melero; Tullio Pozzan; Mario Strazzabosco; Glibenclamide stimulates fluid secretion in rodent cholangiocytes through a cystic fibrosis transmembrane conductance regulator-independent mechanism.. Gastroenterology 2005, 129, 220-233, 10.1053/j.gastro.2005.03.048.
  80. Carlo Spirlı̀; Michael H. Nathanson; Romina Fiorotto; Elena Duner; Lee A. Denson; Juana M Sanz; Francesco Di Virgilio; Lajos Okolicsanyi; Fabio Casagrande; Mario Strazzabosco; Proinflammatory Cytokines Inhibit Secretion in Rat Bile Duct Epithelium. Gastroenterology 2001, 121, 156-169, 10.1053/gast.2001.25516.
  81. Luca Fabris; Massimiliano Cadamuro; Romina Fiorotto; Tania Roskams; Carlo Spirli; Saida Melero; Aurelio Sonzogni; Ruth Joplin; Lajos Okolicsanyi; Mario Strazzabosco; Effects of angiogenic factor overexpression by human and rodent cholangiocytes in polycystic liver diseases. Hepatology 2006, 43, 1001-1012, 10.1002/hep.21143.
  82. Romina Fiorotto; Ambra Villani; Antonis Kourtidis; Roberto Scirpo; Mariangela Amenduni; Peter J. Geibel; Massimiliano Cadamuro; Carlo Spirli; Panos Z. Anastasiadis; Mario Strazzabosco; Massimilano Cadamuro; The cystic fibrosis transmembrane conductance regulator controls biliary epithelial inflammation and permeability by regulating Src tyrosine kinase activity.. Hepatology 2016, 64, 2118-2134, 10.1002/hep.28817.
  83. Ruth Joplin; A. J. Strain; J. M. Neuberger; Biliary epithelial cells from the liver of patients with primary biliary cirrhosis: Isolation, characterization, and short-term culture. The Journal of Pathology 1990, 162, 255-260, 10.1002/path.1711620312.
  84. A. J. Strain; L. Wallace; R. Joplin; Y. Daikuhara; T. Ishii; D. A. Kelly; J. M. Neuberger; Characterization of biliary epithelial cells isolated from needle biopsies of human liver in the presence of hepatocyte growth factor.. The American Journal of Pathology 1995, 146, 537-545, null.
  85. Romina Fiorotto; Mariangela Amenduni; Valeria Mariotti; Luca Fabris; Carlo Spirli; Mario Strazzabosco; Src kinase inhibition reduces inflammatory and cytoskeletal changes in ΔF508 human cholangiocytes and improves cystic fibrosis transmembrane conductance regulator correctors efficacy.. Hepatology 2018, 67, 972-988, 10.1002/hep.29400.
  86. Mercedes Fernandez; Molecular pathophysiology of portal hypertension. Hepatology 2015, 61, 1406-1415, 10.1002/hep.27343.
  87. Patrycja Nowak-Sliwinska; Kari Alitalo6; Elizabeth Allen; Andrey Anisimov; Alfred C. Aplin; Robert Auerbach; Hellmut G. Augustin; David O. Bates; Judy R. Van Beijnum; R. Hugh F. Bender; Gabriele Bergers; Andréas Bikfalvi; Joyce Bischoff; Barbara C. Böck; Peter C. Brooks; F. Bussolino; Bertan Cakir; Peter Carmeliet; Daniel Castranova; Anca M. Cimpean; Ondine Cleaver; George Coukos; George E. Davis; Michele De Palma; Anna Dimberg; Ruud Dings; Valentin Djonov; Andrew C. Dudley; Neil Dufton; Sarah-Maria Fendt; Napoleone Ferrara; Marcus Fruttiger; Dai Fukumura; Bart Ghesquière; Yan Gong; Robert J. Griffin; Adrian L. Harris; Christopher C. W. Hughes; Nan W. Hultgren; M. Luisa Iruela-Arispe; Melita Irving; Rakesh K. Jain; Raghu Kalluri; Joanna Kalucka; Robert S. Kerbel; Jan Kitajewski; Ingeborg Klaassen; Hynda K. Kleinmann; Pieter Koolwijk; Elisabeth Kuczynski; Marc Chanson; Koen Mariën; Juan M. Melero-Martin; Lance L. Munn; Roberto F. Nicosia; Agnes Noel; Jussi Nurro; Anna-Karin Olsson; Tatiana V. Petrova; Kristian Pietras; Roberto Pili; John S. Condeelis; Mark J. Post; Paul H. A. Quax; Gabriel A. Rabinovich; Marius Raica; Anna M. Randi; Menico Ribatti; Curzio Rüegg; Reinier O. Schlingemann; Stefan Schulte-Merker; Lois E. H. Smith; Jonathan W. Song; Steven A. Stacker; Jimmy Stalin; Amber N. Stratman; Maureen Van De Velde; Victor W. M. Van Hinsbergh; Peter B. Vermeulen; Johannes Waltenberger; Brant M. Weinstein; Hong Xin; Bahar Yetkin-Arik; Seppo Ylä-Herttuala; Mervin C. Yoder; Arjan W. Griffioen; Consensus guidelines for the use and interpretation of angiogenesis assays. Angiogenesis 2018, 21, 425-532, 10.1007/s10456-018-9613-x.
  88. Thomas Reiberger; Bernhard Angermayr; Philipp Schwabl; Natascha Rohr-Udilova; Markus Mitterhauser; Alfred Gangl; Markus Peck-Radosavljevic; Sorafenib attenuates the portal hypertensive syndrome in partial portal vein ligated rats. Journal of Hepatology 2009, 51, 865-873, 10.1016/j.jhep.2009.06.024.
  89. Javier Gallego; Ester Garcia-Pras; Marc Mejias; Nuria Pell; Ute Schaeper; Mercedes Fernandez; Therapeutic siRNA targeting endothelial KDR decreases portosystemic collateralization in portal hypertension.. Scientific Reports 2017, 7, 14791, 10.1038/s41598-017-14818-7.
  90. Marc Mejias; L. Coch; A. Berzigotti; Ester García-Pras; J. Gallego; J. Bosch; M. Fernández; Antiangiogenic and antifibrogenic activity of pigment epithelium-derived factor (PEDF) in bile duct-ligated portal hypertensive rats. Gut 2014, 64, 657-666, 10.1136/gutjnl-2014-307138.
  91. Amir Anushiravani; Sadaf Ghajarieh Sepanlou; Burden of Liver Diseases: A Review from Iran. Middle East Journal of Digestive Diseases 2019, 11, 189-191, 10.15171/mejdd.2019.147.
  92. Nympha B. D'souza El-Guindy; Elizabeth J. Kovacs; Philippe De Witte; Claudia Spies; John M. Littleton; Willem J. S. De Villiers; Amanda J. Lott; Timothy P. Plackett; Nadine Lanzke; Gary G. Meadows; Laboratory models available to study alcohol-induced organ damage and immune variations: choosing the appropriate model.. Alcoholism: Clinical and Experimental Research 2010, 34, 1489-511, 10.1111/j.1530-0277.2010.01234.x.
  93. Elizabeth Brandon-Warner; Laura W. Schrum; C. Max Schmidt; Iain H McKillop; Rodent models of alcoholic liver disease: of mice and men.. Alcohol 2012, 46, 715-25, 10.1016/j.alcohol.2012.08.004.
  94. Benedicte Delire; Peter Starkel; Isabelle Leclercq; Animal Models for Fibrotic Liver Diseases: What We Have, What We Need, and What Is under Development. Journal of Clinical and Translational Hepatology 2015, 3, 53-66, 10.14218/JCTH.2014.00035.
  95. Hiroko Itagaki; Kazuhiko Shimizu; Shunichi Morikawa; Kenji Ogawa; Taichi Ezaki; Morphological and functional characterization of non-alcoholic fatty liver disease induced by a methionine-choline-deficient diet in C57BL/6 mice. International journal of clinical and experimental pathology 2013, 6, 2683-2696, null.
  96. Kirstine S. Tølbøl; Birgit Stierstorfer; Jörg F. Rippmann; Sanne Skovgård Veidal; Kristoffer T. G. Rigbolt; Tanja Schönberger; Matthew P. Gillum; Henrik Hansen; Niels Vrang; Jacob Jelsing; Michael Feigh; Andre Broermann; Disease Progression and Pharmacological Intervention in a Nutrient-Deficient Rat Model of Nonalcoholic Steatohepatitis. Digestive Diseases and Sciences 2018, 64, 1238-1256, 10.1007/s10620-018-5395-7.
  97. Kashireddy, P.V.; Rao, M.S. Lack of peroxisome proliferator-activated receptor α in mice enhances methionine and choline deficient diet-induced steatohepatitis. Hepatol. Res. 2004, 30, 104–110. [Google Scholar] [CrossRef]
  98. Samuele De Minicis; Laura Agostinelli; Chiara Rychlicki; Gian Pio Sorice; Stefania Saccomanno; Cinzia Candelaresi; Andrea Giaccari; Luciano Trozzi; Irene Pierantonelli; Eleonora Mingarelli; Marco Marzioni; Giovanna Muscogiuri; Melania Gaggini; Antonio Benedetti; Amalia Gastaldelli; Maria Guido; Gianluca Svegliati-Baroni; HCC Development Is Associated to Peripheral Insulin Resistance in a Mouse Model of NASH. PLOS ONE 2014, 9, e97136, 10.1371/journal.pone.0097136.
  99. Hu-Quan Yin; Mingoo Kim; Ju-Han Kim; Gu Kong; Mi-Ock Lee; Kyung-Sun Kang; Byung-Il Yoon; Hyung-Lae Kim; Byung-Hoon Lee; Hepatic gene expression profiling and lipid homeostasis in mice exposed to steatogenic drug, tetracycline.. Toxicological Sciences 2006, 94, , 10.1093/toxsci/kfl078.
  100. Sayantan Nath; Sankar Kumar Ghosh; Yashmin Choudhury; A murine model of type 2 diabetes mellitus developed using a combination of high fat diet and multiple low doses of streptozotocin treatment mimics the metabolic characteristics of type 2 diabetes mellitus in humans. Journal of Pharmacological and Toxicological Methods 2017, 84, 20-30, 10.1016/j.vascn.2016.10.007.
  101. Martijn W H Leenders; Maarten W Nijkamp; Inne H M Borel Rinkes; Mouse models in liver cancer research: a review of current literature.. World Journal of Gastroenterology 2008, 14, , null.
  102. Dianne H. Dapito; Ali Mencin; Geum-Youn Gwak; Jean-Philippe Pradère; Myoung-Kuk Jang; Ingmar Mederacke; Jorge M. Caviglia; Hossein Khiabanian; Adebowale Adeyemi; Ramon Bataller; Jay H. Lefkowitch; Maureen Bower; Richard Friedman; R. Balfour Sartor; Raul Rabadan; Robert F. Schwabe; Promotion of Hepatocellular Carcinoma by the Intestinal Microbiota and TLR4. Cancer Cell 2012, 21, 504-16, 10.1016/j.ccr.2012.02.007.
  103. Michelle Dow; Rachel M. Pyke; Brian Y. Tsui; Ludmil B. Alexandrov; Hayato Nakagawa; Koji Taniguchi; Ekihiro Seki; Olivier Harismendy; Shabnam Shalapour; Michael Karin; Hannah Carter; Joan Font-Burgada; Integrative genomic analysis of mouse and human hepatocellular carcinoma. Proceedings of the National Academy of Sciences 2018, 115, E9879-E9888, 10.1073/pnas.1811029115.
  104. Ju-Seog Lee; In-Sun Chu; Arsen Mikaelyan; Diego F Calvisi; Jeonghoon Heo; Janardan K Reddy; Snorri S Thorgeirsson; Application of comparative functional genomics to identify best-fit mouse models to study human cancer. Nature Genetics 2004, 36, 1306-1311, 10.1038/ng1481.
  105. Pimpin, L.; Cortez-Pinto, H.; Negro, F.; Corbould, E.; Lazarus, J.V.; Webber, L.; Sheron, N. EASL HEPAHEALTH Steering Committee Burden of liver disease in Europe: Epidemiology and analysis of risk factors to identify prevention policies. J. Hepatol. 2018, 69, 718–735. [Google Scholar] [CrossRef]
  106. Thomas Reiberger; Mattias Mandorfer; Beta adrenergic blockade and decompensated cirrhosis. Journal of Hepatology 2017, 66, 849-859, 10.1016/j.jhep.2016.11.001.
  107. Directive 2010/63/EU of the European Parliament and of the Council on the protection of animals used for scientific purposes. Official Journal of the European Union. 2010. L276/33–L276/79 (22 September 2010). Available online: http://eur-lex.europa.eu/LexUriServ (accessed on 5 November 2019).
  108. EARA Statement supporting European Directive 2010/63/EU on the protection of animals used for scientific purposes n.d. Available online: http://www.ehnheart.org/projects/1037:eara-statement-supporting-european-directive-201063eu-on-the-protection-of-animals-used-for-scient (accessed on 5 November 2019).
  109. FELASA working group on revision of guidelines for health monitoring of rodents and rabbits; M Mähler (Convenor); M Berard; R Feinstein; A Gallagher; B Illgen-Wilcke; K Pritchett-Corning; Marcello Raspa; FELASA recommendations for the health monitoring of mouse, rat, hamster, guinea pig and rabbit colonies in breeding and experimental units. Laboratory Animals 2014, 48, 178-192, 10.1177/0023677213516312.
  110. Russell, W.M.S.; Burch, R. The Principles of Humane Experimental Technique; Methuen: London, UK, 1959. [Google Scholar]
  111. Sara Gargiulo; Adelaide Greco; Matteo Gramanzini; Silvia Esposito; Andrea Affuso; A. Brunetti; Giancarlo Vesce; Mice Anesthesia, Analgesia, and Care, Part I: Anesthetic Considerations in Preclinical Research. The ILAR Journal 2012, 53, , 10.1093/ilar.53.1.55.
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