Interactions of oxygen molecules with electrons leaked from enzymatic and non-enzymatic processes produces reactive oxygen species (ROS), such as superoxide (O₂⁻•) and hydrogen peroxides (H₂O₂) ^[1][2][3][1,2,3]. Hydroxyl radicals (HO•) are the most reactive type of ROS and are likely produced by the reaction of H₂O₂ and ferrous iron, via the so called Fenton reaction [4], although there is some debate as to which ROS are the primary products in this reaction ^[5][6][5,6]. Singlet molecular oxygen (¹O₂), is a high-energy oxygen species but possesses unique properties that are different from other ROS ^[7][8][9][7,8,9]. While most ROS are produced by electron transfer reactions, ¹O₂ is generated by the transfer of energy to the ground state, triplet molecular oxygen (³O₂), the most abundant oxygen molecule in atmosphere. The type II photodynamic reaction promoted by the presence of photosensitizing molecules is widely employed to generate ¹O₂ in biological systems ^[10][11][10,11]. However, the problem with the photodynamic action is that other ROS are also generated as byproducts ^[12][13][12,13]. This methodological issue appears to have hindered progress in research on the biological effects of ¹O₂, despite its importance.

¹O₂ possesses high energy and is considered to be a major cause for skin damage induced by ultraviolet (UV) irradiation ^[14][15][14,15]. Meanwhile, due to its strong cytotoxicity, ¹O₂ is the molecule that is responsible for killing tumor cells during photodynamic therapy (PDT) ^[10][11][10,11]. ¹O₂ as well as HO• preferentially reacts with conjugated double bonds, and hence polyunsaturated fatty acids (PUFA), which are dominantly present in the form of phospholipids in the cell membrane, are likely targets [9]. It is known that, upon mitotic stimuli, a small amount of ROS, notably H₂O₂, is produced, and this species modulates phosphorylation-mediated signaling pathways ^[16][17][16,17]. While signal modulation by H₂O₂ involves the transient oxidation of cysteine (Cys), reactions with ¹O₂ tend to result in irreversible oxidation. In most cases, exposure to ¹O₂ impairs cellular function but also occasionally stimulates tumorigenic cell growth ^[18][19][18,19]. Concerning cell death, results reported in many studies indicate that the apoptotic pathway is activated by ¹O₂ ^[20][21][22][20,21,22]. However, recent studies suggest that ferroptosis, an iron-dependent necrotic cell death ^[23][24][23,24], is also involved in ¹O₂-promoted cell death ^[25][26][25,26].

Oxidative stress is induced by either the production of large amounts of ROS or an insufficient amount of antioxidants which include enzymes that eliminate ROS or small antioxidant compounds, such as glutathione (GSH), carotenoids and tocopherols [2]. Electrons that are leaked from enzymatic and non-enzymatic reactions initiate the generation of ROS, as represented by O₂⁻•, H₂O₂ and HO•, and hence, the radical electron plays pivotal roles in the development of oxidative stress in many situations ^[1][3][1,3]. However, ¹O₂ is generated when the oxygen molecule in the ground triplet state ³O₂ is excited by receiving energy without the transfer of an electron. The ¹O₂-generating system involves enzymatic reactions, such as myeloperoxidase, lipoxygenase and cyclooxygenase as well as chemical reactions, such as O₂⁻•-mediated GSH oxidation and the interaction of peroxides with hypochlorite or peroxynitrite ^{[13][27][28][29]}[13,28,29,30]. Photoaging and PDT are the subjects that have been most extensively investigated in terms of ¹O₂–mediated reactions that are associated with human physiology and the pathogenesis of related diseases. In spite of the high oxidizing power, reactions of ¹O₂ are thought to exert only limited effects compared to those of HO• in biological systems.

HO• is considered to be the most reactive ROS and appears to be responsible for a variety of pathological conditions. However, the half-life of HO• is quite short (10 nsec), so it only reacts with molecules that are in close proximity to the site where it is generated. On the other hand, the half-life of ¹O₂ is approximately 4 µsec in aqueous solution, which allows it to diffuse 150–220 nm ^[30][31][31,32]. Thus, ¹O₂ may react at various locations beyond where it is generated and, therefore, can affect surrounding molecules and organelles more widely compared to HO•. Nevertheless, this distance is insufficient for extracellularly produced ¹O₂ to move to the interior of a cell. Therefore, ¹O₂ that is generated inside the cell has the ability to damage various cellular components including DNA and organelles.

Analyses employing a cell biological approach are essential for answering basic questions as to which part of the cell produces ¹O₂ in photoaging and during PDT and how cellular responses proceed in such situations. For that purpose, the use of a fluorescent chemical probe is the most convenient approach. ¹O₂ sensor green (SOSG) is a prototype that is popularly used in studies for detecting ¹O₂, although it has some disadvantages such as lack in membrane permeability ^[32][33]. Other compounds have been designed to overcome the disadvantage of SOSG. For example, Aarhus Sensor Green, which is a tetrafluoro-substituted fluorescein derivative that is covalently linked to a 9,10-diphenyl anthracene moiety ^[33][34] and the classic indocyanine green probe may also be applicable for this objective in certain experiments ^[34][35]. To increase the cellular delivery of SOSG, biocompatible nanosensors, with SOSG encapsulated within their hydrophobic core, have been developed, and these modifications appear to improve its delivery ^[35][36][37][36,37,38].

The cell membrane permeable far-red fluorescence probe Si-DMA, which is composed of silicon-containing rhodamine and anthracene moieties as a chromophore, has also been developed ^[38][39]. Upon reaction with ¹O₂, Si-DMA is converted into an endoperoxide at the anthracene moiety that emits strongly. The use of Si-DMA reportedly enables the visualization of ¹O₂ generated in a single mitochondrial tubule during PDT. After the treatment of cells with the endoperoxide, dose-dependent increases in fluorescence of Si-DMA were observed ^[39][40]. Thus, these results suggest that chemical probes may be applicable for studies concerning the cellular effects of ¹O₂. This compound is now commercially available. Another compound, a rhodamine 6G-aminomethylanthracene-linked donor–acceptor molecule (RA), was reported to exhibit unique properties ^[40][41]. RA acts as a fluorogenic ¹O₂ sensor molecule and also acts as a photosensitizer to generate ¹O₂ upon exposure to green light. Other fluorescent reagents, such as one based on an aminocoumarin-methylanthracene-based electron donor–acceptor molecule ^[41][42], are also being developed. Since information on the use of these newly developed probes is currently limited, it is necessary to carefully choose which compounds are suitable for the intended research.

PDT mainly contributes to ¹O₂ generation in biological systems through type II mechanisms that involve energy transfer from triplet excited molecules to triplet oxygen. Photosensitizers may also act according to competitive type I photosensitization mechanism that mostly involves charge transfer between suitable targets and a photosensitizer in its triplet excited state ^[42][43][43,44]. In order to protect cells against the deteriorating action of UV light, the effects of ¹O₂ on skin tissues have been extensively investigated. In the meantime, ¹O₂ is considered to be the main molecule that promotes cytotoxic processes during PDT, and hence, multiple studies are currently underway with the aim of understanding the mechanism responsible for ¹O₂-mediated cell death and developing efficient photosensitizers ^[8][10][8,10]. UV radiation causes skin photoaging and oxidatively generated damage to dermal cells and is especially troublesome in cases of sunburn which occurs by exposure to excessive UV for long periods of time ^[14][44][14,45]. UVB (280–315 nm) comprises approximately 5% of the solar UV and causes the direct photodamage to many molecules including DNA and proteins in skin tissues through its high energy photochemical reactions. Genetic damage caused by the oxidative modification of DNA and other molecules emerges in a short time after exposure to UVB. In the case of UVA (315–400 nm) that accounts for approximately 95% of the solar UV, cellular damage occurs through the activation of chromophores that act as photosensitizers to generate ¹O₂ and other ROS, and hence, the oxidative reaction proceeds indirectly via the ROS that are generated. It is rather difficult to determine if changes in cells that have been exposed to UVA are the consequence of the generation of either ¹O₂ or other ROS because they are produced simultaneously by the photodynamic reactions and result in essentially the same end products ^[45][46].

In order to elucidate the reactions caused by ¹O₂, reliable methods for generating ¹O are required [12]. The most common method for this purpose is irradiation of the photosensitizer with UV or visible light because it is simple and easy to control its production ^[46][47][27,47]. Figure 1 represents the “Jablonski diagram” that depicts conceptual images of the generation of ¹O₂ by the irradiation of a photosensitizing molecule (S) with light followed by transferring energy to ³O₂ [48]. When a photosensitizer is exposed to light, most likely the UVA in natural light, photon energy converts the photosensitizer in the ground state (⁰S) to that in the excited state (¹S). On returning to the ground state ⁰S, a part of the released photoenergy can be transferred to ³O₂, which results in the electron spin state being altered and the generation of ¹O₂. Under this situation, photodynamic action generates not only ¹O₂ but also other ROS such as O₂⁻• and HO• [8].

To observe cellular responses to ¹O₂, cell-permeable and non-cytotoxic compounds need to be used as the photosensitizer. For example, Rose Bengal and methylene blue meet the conditions and, hence, are popularly used for the purpose of examining biological action of ¹O₂ [47]. Since PDT is a useful therapeutic for eliminating tumors, many attempts have been made to improve the treatment by developing convenient photosensitizers ^[11][46][49][11,27,49].

Endoperoxides that release ¹O₂ without other ROS have been developed to evaluate its unique reaction ^[13][50][13,50]. Naphthalene endoperoxide-based ¹O₂ donor compounds were first developed, and the structure–function relationships of the compounds have been described in detail in a review article [13]. Consequently, several naphthalene endoperoxides have been established and utilized for the in vitro evaluation of the effects of ¹O₂, as representative structures in Figure 2A. Upon mild heating at 37 °C, the endoperoxides spontaneously release pure ¹O₂, which then directly reacts with surrounding compounds and organelles. Heat-labile endoperoxides are considered clean sources of ¹O₂ for highly specific oxidation of cellular biomolecules and have been applied for ¹O₂-mediated oxidation of the DNA guanine base in cells ^[51][52][51,52].

Here discuss the advantages and disadvantages of ¹O₂ donor compounds in comparison with the photodynamic action. The benefits include the following: (1) Endoperoxides produce pure ¹O₂. (2) The concentrations of the released ¹O₂ are easily controlled. (3) Heating at physiological temperature, generally under cell culture conditions, can promote the release of ¹O₂ from endoperoxides. (4) It may be possible to design endoperoxides that are localized to a specific organelle by appropriate chemical modification of the compounds. Limitations include the following: (1) A high concentration of endoperoxides is required to generate sufficient levels of ¹O₂. (2) It is essential to consider effects of the raw material after the release of ¹O₂ because they are sometimes toxic to cells. (3) Endoperoxides may not be evenly distributed inside cells due to their chemical nature. (4) The amount of ¹O₂ released is initially maximal but then gradually decreases with increasing endoperoxide consumption.

When endoperoxides are applied to a cell culture system, it is necessary to use compounds that are able to pass through the cell membrane. In fact, the side chains of naphthalene endoperoxides determine whether they enter cells or remain outside of cells (Figure 2B) [53]. 1-Methylnaphthalene-4-propanonate endoperoxide (MNPO₂) is cell membrane-permeable and generates ¹O₂ within cells. However, 1,4-naphthalenedipropanoate endoperoxide (NDPO₂) cannot enter cells. Accordingly, while MNPO₂ induces cell damage, NDPO₂ at the same concentration has no effects, although both compounds trigger the release of cyt c from isolated mitochondria to a similar extent [53].

While the generation of ¹O₂ by a light-irradiated photosensitizer is frequently used, the use of ¹O₂ donor compounds has been limited because they are complex molecules that are difficult to synthesize. Some naphthalene endoperoxides are now commercially available. New compounds other than naphthalene-based endoperoxides are also being developed. For the efficient delivery of a ¹O₂ donor to cancer cells, a porphyrin-based covalent organic framework that contains a naphthalene endoperoxide has also been developed [54]. Trials to develop new types of endoperoxides, which are based on 2-pyridone and anthracene, are also underway [50]. Two ¹O₂-producing systems, photodynamic reactions and naphthalene endoperoxides, have provided rather consistent results so far [13], implying that the contribution of other byproducts may be negligible.

The body is protected from oxidatively generated damage by a variety of natural and synthetic compounds that scavenge ¹O₂. The quantitative evaluation of the ¹O₂-scavenging ability of a compound provides useful information not only for basic research but also for developing functional foods and medicines concerning antioxidation [55]. Many nutritional compounds, such as tocopherols, carotenoids and flavonoids, possess antioxidant capacity and protect susceptible molecules from ¹O₂. The oxygen radical absorption capacity (ORAC) assay is a representative method for the detection of ¹O₂-scavenging ability of food ingredients [56]. Thereafter, a simple method called a singlet oxygen absorption capacity (SOAC) assay has been established for the evaluation of ¹O₂-scavenging ability ^[56][57][56,57]. These methods are useful in exploring popularly used ¹O₂-scavenging compounds, especially in the field of food chemistry.

While carotenoids react with ¹O₂ more rapidly than α-tocopherol, in a nearly diffusion-limited manner (~10¹⁰ M⁻¹s⁻¹) ^[58][59][58,59], lycopene, which is found in fruits and vegetables such as tomato, is one of the strongest natural carotenoids [60]. By employing the SOAC assay method, carotenoids have been found to quench ¹O₂ approximately 30–100 times faster than α-tocopherol [57]. After transferring excitation energy to carotenoids, ¹O₂ returns to the ground state. The excited carotenoids spontaneously release thermal energy and then return to the ground state. Hence, carotenoids are spontaneously recycled and have the advantage of quenching ¹O₂ without affecting other molecules. This chemical property of ¹O₂ is a major difference from other radicals that are generated by electron transfer reactions that require another radical to quench.

Based on in vitro data on the action of anti-oxidants, the biological benefit of carotenoids has also been examined by some studies on humans. The administration of a representative carotenoid β-carotene to humans failed to alleviate sunburn reactions under the conditions used [61]. However, a later study reported that carotenoids, β-carotene and lycopene effectively protect erythema formation induced using a solar light simulator ^[62][63][62,63]. Lycopene has also attracted attention as a nutrient with anticancer effects [64]. For another example, lutein is a xanthophyll carotenoid that is found in foods such as dark green leafy vegetables and exhibits strong antioxidant activity via its ability to scavenge ROS including ¹O₂ and lipid peroxyl radicals [65]. Lutein appears to exert anti-inflammatory actions against some diseases, including neurodegenerative disorders, eye diseases, cardiovascular diseases and skin diseases.

Many synthetic antioxidant compounds that scavenge ROS including ¹O₂ have been developed as medicines. Edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one) is a compound that eliminates a variety of radical species and was the first approved compound for use as a medicine for the treatment of acute brain infarctions. Edaravone can scavenge ¹O₂ that is generated by activated human neutrophils [66] and by photoactivated Rose Bengal [67]. The plasma lipid peroxidation caused by ¹O₂, however, cannot be suppressed by edaravone and other clinical drugs with antioxidant ability, which include roglitazone, probucol, carvedilol, pentoxifylline and ebselen, although they exhibit suppressive effects on lipid peroxidation caused by free radicals, peroxynitrite, hypochlorite, and lipoxygenase reactions [68]. Because blood plasma contains high concentrations of proteins and many other compounds that could potentially interfere with the scavenging reaction by these chemicals, such biological compounds may have influenced the results.