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Gastric cancer (GC) remains a significant global health challenge, with late-stage diagnosis impacting treatment options and decreased survival rates. To address this, there has been a growing interest in the development of innovative screening and diagnostic methods. Nanobiosensors have undergone multiple iterations and unveiled remarkable features that pledge to reshape patient care.
Sensing Platform | Transducer | Biomarker | Human Sample | LoD | Takeaways | Ref. |
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High-density “hot spot” AuNPs@SiO array substrate with RCA strategy | Optical (SERS) | M.SssI | Serum | 2.51 × 10−4 U mL−1 | Simple preparation, high biocompatibility, uniformity, reproducibility, stability | [30] |
Polymeric l-arginine and rGO-AuNSs on glass electrode | Electrochemical (CV) | PIK3CA ctDNA | 1.0 × 10−20 M | Label-free, desirable stability, wide dynamic response | [31] | |
SWCN DMEJ with DNA–gold urchin | Electrochemical (IDE) | SOX-17 | 1 aM | High performance, efficiency, biocompatibility, no cross-reactivity | [32] | |
Nitrophenyl-functionalized black phosphorus nanosheets and FAM labelling | Optical (fluorescence) | PIK3CA E542K ctDNA | Tumor cell lines | 50 fM | Enzyme-free, long-term stability, simple manufacturing process, good discrimination ability of interferences | [33] |
Nanoplasmonic, nanogold-linked sorbent assay | Optical (FOPPR and FONLISA) | Methylated SOCS-1 | Tumor tissue and cell lines | 0.81 fM | PCR- and amplification-free, label- and sequencing-free; superior to PCR and other assays | [34] |
Sensing Platform | Transducer | Biomarker | Human Sample | LoD | Takeaways | Ref. |
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Blackberry-like magnetic DNA/FMMA nanospheres on gold stir-bar using CHA-HCR and RAFT amplification | Electrochemical (V) | miR-106a | Serum | 0.68 aM | Enzyme-free, simple nanomaterials, acceptable storage stability, RNA extraction-free, sample pretreatment-free technique, high recovery | [49] |
Gold–magnetic NPs single-strand (ss) probe 1 (P1) | Electrochemical (EIS, CV, DPV) | miR-106a | Serum | 0.3 fM | Great performance, stability, simplicity, reproducibility, agreeable storage stability | [50] |
AuNPs and CdSe@CdS QDs-contained magnetic nanocomposites labels with polythiophene/rGO-modified carbon electrodes | Electrochemical (CV, DPV) | miR-106a let-7a |
Plasma | 0.06 fM (miR-106a) 0.02 fM (let-7a) |
Multiplexing, good recovery, reproducibility, appropriate storage stability | [51] |
AgNRs array coated by the mF-MoS2 NSs, dual mode detection assay | Optical (SERS) and electrochemical (SWV) | miR-106a | Serum | 67.44 fM 248.01 fM |
In situ, stability, reliability, reproducibility, minimal interference | [52] |
Perovskite–graphene oxide nanocomposite on an electrode, genosensing assay | Electrochemical (chronoamperometry) | miR-21 | Cell lines | 2.94 fM | Label-free, reproducibility, reusability, stability, versatility, robustness | [53] |
Ratiometric strategy using CDs with triple function and FAM-labeled ssDNA | Optical (fluorescence) | miR-21 | Plasma | 1 pM | Reproducibility, reliability, simplicity, strong anti-interference ability, excellent performance | [54] |
Two-stage cyclic enzymatic amplification with T4 RNA ligase 2 and T7 exonuclease and AuNPs | Electrochemical (DPV) | miR-21 | Serum | 0.36 fM | Convenience, reproducibility, excellent performance, stability | [55] |
MXene-derivative QDs (Mo2TiC2 QDs) and SnS2 nanosheets/lipid bilayer | Electrochemical and optical (voltammetry and fluorescence) | miR-27a-3p | Ascites | 1 fM | Reproducibility, low background noise, wide dynamic range, good stability, minimal interference | [56] |
“Hot spot” bismuth nano-nest/Ti3CN QD- SPC-ECL | Electrochemical and optical (voltammetry and fluorescence) | miR-421 | Ascites | 0.3 fM | Improved luminescence and catalytic activity, stability, controllability | [57] |
Dual-response–single-amplification nanomachine | Optical (fluorescence) | miR-5585-5p & PLS3 mRNA | Serum | 1.19 fM (miR-5585-5p) 16.37 fM (PLS3) |
Enzyme-free, extraction-free, high recovery, great performance | [58] |
CPs/AuNP-AuE with DSN | Electrochemical (chronoamperometry and CV) | miR-100 | Serum | 100 aM | Enzyme-free, reliability, controllability, effectiveness | [59] |
Sensing Platform | Transducer | Biomarker | Human Sample | LoD | Takeaways | Ref. |
---|---|---|---|---|---|---|
MoS2 QDs-MXene heterostructure and AuNPs@biomimetic lipid layer | Electrochemical and optical (V and fluorescence) | Exosomal miR-135b | Ascites | 10 fM | Versatility, reproducibility, reliability, low background noise, high accuracy; large surface area, excellent flexibility and superior conductivity of substrates, excellent antifouling property | [68] |
“Hot spot” AuNSs-decorated MoS2 nanocomposite (MoS2-AuNSs) aptasensors | Optical (SERS) | CD63 of exosomes | Serum | 17 particles μL−1 | Reliability, reproducibility, good stability long term, excellent Raman enhancement effect and generability in bioanalysis | [14] |