Submitted Successfully!
To reward your contribution, here is a gift for you: A free trial for our video production service.
Thank you for your contribution! You can also upload a video entry or images related to this topic.
Version Summary Created by Modification Content Size Created at Operation
1 -- 2479 2023-06-15 13:20:55 |
2 format change Meta information modification 2479 2023-06-16 03:58:47 |

Video Upload Options

Do you have a full video?


Are you sure to Delete?
If you have any further questions, please contact Encyclopedia Editorial Office.
Řepka, D.; Kurillová, A.; Murtaja, Y.; Lapčík, L. Physical-Chemical Active Substances Encapsulation Approaches in Pharmaceutical Industry. Encyclopedia. Available online: (accessed on 16 June 2024).
Řepka D, Kurillová A, Murtaja Y, Lapčík L. Physical-Chemical Active Substances Encapsulation Approaches in Pharmaceutical Industry. Encyclopedia. Available at: Accessed June 16, 2024.
Řepka, David, Antónia Kurillová, Yousef Murtaja, Lubomír Lapčík. "Physical-Chemical Active Substances Encapsulation Approaches in Pharmaceutical Industry" Encyclopedia, (accessed June 16, 2024).
Řepka, D., Kurillová, A., Murtaja, Y., & Lapčík, L. (2023, June 15). Physical-Chemical Active Substances Encapsulation Approaches in Pharmaceutical Industry. In Encyclopedia.
Řepka, David, et al. "Physical-Chemical Active Substances Encapsulation Approaches in Pharmaceutical Industry." Encyclopedia. Web. 15 June, 2023.
Physical-Chemical Active Substances Encapsulation Approaches in Pharmaceutical Industry

Encapsulation is a valuable method used to protect active substances and enhance their physico-chemical properties. It can also be used as protection from unpleasant scents and flavors or adverse environmental conditions. Encapsulation has demonstrated effectiveness and versatility in multiple industries, such as food, nutraceutical, and pharmaceuticals. Moreover, the selection of appropriate encapsulation methods is critical for the effective encapsulation of specific active compounds. 

encapsulation pharmaceutical physico-chemical properties

1. Introduction

Different substances have great health benefits or function as a drug, but they cannot be used directly or stored for a long period. This is due to their low solubility, low bio-availability, tendency to oxidize, or their strong odor or flavor. Encapsulation is a method that has been known for more than 60 years, but it still holds great interest among researchers [1][2]. Typically, a substance is enclosed by a coating material which forms a barrier to protect the substance from the environment and chemical interaction [3][4][5][6]. Encapsulated substances can be also called core, fill, or matrix substances. The coating material is called a shell or wall material, as can be seen in Figure 1. Encapsulation can be used for improving the mentioned properties of different substances as well as protecting the core substance from, for example, light, moisture, and changes in pH [2][7][8][9]. Apart from protecting of core, it can also be used in the prolongation of release. This can be helpful with gradual and controlled release of drugs [10][11].
Figure 1. Substance encapsulated into a coating material.
Encapsulation can be achieved by many methods, some of which are described in this entry. These methods can be divided into three main types: chemical, physico-chemical, and physico-mechanical [7][12]. This entry does not mention every encapsulation approach since it is a very broad topic, but rather focuses on methods mostly used in pharmaceutical and food industries. The global food encapsulation market has undergone continuous growth and is expected to reach a value of USD 17 billion by 2027 [13].
With regard to encapsulation of pharmacologically active substances, it is important to note that this technology can have broader applications beyond just the pharmaceutical and food industries. Encapsulation can also be utilized in various other industries; for example, it can be used in nutraceuticals or in the construction industry as a self-healing material such as concrete [14][15]. Specifically, in concrete, types of bacteria can be encapsulated for a self-healing effect [16]. The principle of self-healing materials is applied when the treated material is mechanically damaged; the encapsulate is released after a rupture of capsules and the core material is used to repair the damaged part [17][18][19]. In the same industry, the encapsulation is also used for anticorrosive coating. An example of encapsulated food is linseed oil [20]. The anticorrosive coating can have three mechanisms of protection. Firstly, it is a basic barrier on top of the material; secondly, the coating material can carry corrosion inhibitors; lastly, the coating can provide cathodic protection [21]. Self-healing materials and anticorrosion coatings of materials aim to prolong material use and prevent and avoid structural damage that may occur [22]. For example, marine infrastructure such as bridges and tunnels are in hostile environments and are prone to micro-cracks [23].
Quantum dots (QDs) are closely examined within chemistry. QDs are between 2 and 10 nm in diameter and have a semiconductor core with a shell, together with ligands [24]. Quantum dots can be also used together with encapsulation methods to achieve better properties, which are already unique. Quantum dots are widely used in the biomedical industry [24][25], for example, in biomolecular tracking, tumor imaging, and photodynamic therapy [24]. Encapsulated QDs can be used for the detection of heavy metals and other possible harmful substances [26]. Table 1 provides specific examples of the encapsulation methods described in this entry, along with several examples from other industries that demonstrate the diversity of encapsulation.
Table 1. Different methods of encapsulation.







Field of


Enhanced Property


Emulsion electro-spraying assisted by pressurized gas

Algae oil

Wheat gluten extract

3.34 ± 1.77 µm

Nutraceutical industry

Oxidation, bioavailability, organoleptic properties, controlled




Polyphenols of Piper Betel leaves



Nutraceutical industry, food supplementation industry


oxidation, taste


Polyphenols from Mesona chinensis Benth extract


1516.67 ± 40.96 µm

Traditional medicine

Bioaccessibility, bioavailability


Phage SL01


2.110 ± 0.291–2.982 ± 0.477 mm

Pharmaceutical industry

Bioavailability, better survivability (pH, enzymes)



Thyme oil


50.18 ± 2.32 nm

Bioinsecticides, larvicides





Medium-chain triglyceride

108–166 nm

Food application

Water solubility, bioavailability




Sunflower oil, carboxy-methylcellu-lose, lecithin

~20 mm

Food delivery

Bioavailability, photochemical stability, less degradation


Spray drying




Nutraceutical industry, colorant

Shelf life,



Saccharomyces boulardii

Rice protein, maltodextrin


Functional foods and beverages, supplements, animal feed

Effectiveness, prolongue storage, less degradation


Freeze drying

Blackthorn (Prunus spinosa L.) extract



Functional foods, supplements, pharmaceutical


bioavailability, physico-chemical and biological




Whey protein isolate

99.76 ± 21.56–242.22 ± 81.78 nm

Alternative medicine, food, cosmetic and pharmaceutical industries

Odor, taste,






Stearic acid, sunflower oil/rice bran phospholipids, Tween 80

143 ± 3.46 nm

Nutraceutical industry


solubility, size


Internal phase separation



100–1000 µm

Nutritional supplement



astringency, sulfur smell


Self-assembly of biopolymers


Whey protein isolate, pectin

~ 200 nm

Nutraceutical industry, colorant




Vacuum facilitated infusion


Geotrichum candidum arthrospores


Food industry, pharmaceutical industry

Water solubility, chemical stability,



Multiple step preparation including modified Störber sol-gel process


Hollow carbon sphere coated by graphene layer


Battery industry

Enhancing performance of lithium-ion batteries,




Synthesis of QD, growth of iron shell, and oxidation to form iron oxide shell

Quantum dots

Iron oxide

~20 nm

Bifunctional markers, virus detection




Pelletization process,



Calcium acetate/sodium carbonate (or composite of two), superabsorbent

polymers, poly(ethylene glycol)

Epoxy resin, fine sand


Self-healing concrete

Waterproof and alkali


mineralization time,



Sol-gel method






energy, UV-protecting

Photoactivity properties


2. Physical-Chemical Approaches in Pharmaceutical Industry

2.1. Encapsulation of Bacteriophages

Encapsulation can be used for the treatment of bacterial infections [46]. Due to the still-greater risk of bacteria obtaining resistance to antibiotics, the encapsulation of bacteriophages can be one of the main options to fight bacterial infection without antibiotics [46]. Bacteriophages are viruses that can infect and kill bacteria with high specificity and do not threaten healthy human microflora [46][47][48][49]. Without encapsulation, the bacteriophages have lower survivability in gastro-intestinal conditions; specifically, they can be affected by gastric enzymes, bile, and the acidic environment when used orally [46][48][50].

2.1.1. Emulsification

This method is based on dispersing one liquid with the active compound into a second liquid that is not miscible, and creating small droplets [51][52][53][54][55]. Emulsions tend to be thermodynamically unstable and surfactants as emulsifiers need to be added to decrease surface tension [56][57][58]. Solid particles, such as nanoparticles, can also function as a stabilizer and emulsions stabilized in such a way are called Pickering emulsions [56][58][59].
The method mentioned by Anna Choinska-Pulit et al. includes a mixture of microorganism cells and polymer, which is added to vegetable oil (canola, sunflower, and corn oil, for example) [46]. Mixtures must be homogenous until a water-in-oil emulsion is formed, and stirring of the emulsion is a key step to obtain droplets having the right size and shape. Emulsification creates droplets called capsules, whereas extrusion droplets are called beads. The capsule core is liquid, and the bead core is porous. Capsules are at least 100 times smaller than beads [60].
Emulsifiers, for example, guar gum or lecithin, must be included for stabilization. Settling is used to recover hardened capsules. Researchers in the publication from Dini et al. used this method to encapsulate bacteriophages to reduce enterohemorrhagic E. coli in the bovine gastric environment; methoxylated pectin was used as the material for encapsulation and was emulsified by mixing with Tween 20 (Polyoxyethylene sorbitan monolaurate) [61]. Homogenization was performed by mixing and oleic acid was added to reach a final concentration of 10 vol%. Additionally, coating was carried out with 0.2 wt% of high-methoxylated pectin or guar gum.
Double water-in-oil-in-water (W/O/W) emulsion was conducted by Kim, S. et al. [62]. PLGA microspheres were prepared using a bacteriophage solution of pAh-6C in phosphate buffered saline (PBS), mixed with PLGA, and dissolved in dichloromethane to form a W/O emulsion. To form a double emulsion, the primary W/O was homogenized together with polyvinyl alcohol (PVA). A second set of microspheres comprised PLGA/alginate composite. This composite was prepared by preparing W/O/W as before. Calcium chloride solution was added and homogenized to crosslink the alginate. To the emulsion was added deionized water and, after stirring and evaporating, microspheres were obtained [62].

2.1.2. Extrusion

The principle of this method is the forceful flow of material through a slit [8]. Extrusion is a suitable method for the preparation of capsules with hydrocolloids by adding microorganisms. The cell suspension is extruded through a syringe needle in the form of droplets into a bath or a hardening solution, which is mostly calcium chloride [46][63][64][65].
The group of Zhenxing Tang et al. used extrusion as the method to encapsulate Felix O1 bacteriophages into alginate-whey protein microspheres [66]. In this work, researchers focused on the growing problem of bacterial contamination in food poisoning. Worldwide treatment of bacterial infections in food animals is carried out using antibiotics, which causes the overuse of these drugs. Bacteriophage treatment can be a good substitute. Encapsulation is needed because gastric acidity decreases the viability of bacteriophages. Within minutes, the phages were found to be inactive in the simulated gastric fluid. The viability of Felix O1 increased to 2 h of incubation in alginate-whey protein microcapsules [66]. Encapsulation efficiency describes how much of the core material is successfully entrapped into the capsules. The encapsulation efficiency of bacteriophages is calculated as the quantity released from capsules divided by the initial quantity in the capsules multiplied by 100% [67]:
E E = ( m s m t ) · 100 %
where 𝑚𝑖 is the initial mass and 𝑚𝑠 is the mass of the compound left in solution. Here the encapsulation efficiency reached 99% for mixtures of alginate-whey proteins from 93% of pure alginate microspheres. The extrusion method is simple and cheap but has a big disadvantage for use at a mass-production scale [46].
In the publication by Savic et al., researchers encapsulated extracted antioxidants from orange peel into alginate-chitosan microparticles [68]. The extrusion method with coaxial airflow was used for encapsulation. To the solution of alginate 1.5% (v/v) was added ethanol extract of orange peels. From the homogeneous solution, which was in a plastic syringe, drops were torn using coaxial airflow. Droplets fell into the crosslinking solution while stirring and solidifying. The crosslinking solution was prepared using calcium chloride 2% (w/v) and chitosan 0.5% (w/v). The chitosan was prepared using 0.5% (v/v) acetic acid. The encapsulation efficiency was 89.2% [68].

2.2. Probiotic Encapsulation by Chitosan-Gel Particles

Probiotics are helpful bacteria for maintaining a healthy bowel environment. A problem in administering probiotics is that they are prone to degradation because of humidity and low pH in the human intestines. The group of Albadran H. et al. devised a novel method to encapsulate probiotics in chitosan-coated agar-gelatin particles for releasing probiotics into the large intestine [69]. This method should be scalable and thus more suitable than commonly used extrusion methods. Firstly, they prepared agar-gelatin particles loaded with bacteria. This was undertaken by separately dissolving agar and gelatin in deionized water for 2 h at 70–80 °C. Both substances were mixed together at a 1:1 ratio and autoclaved. A small volume of cell suspension was mixed with agar-gelatin and poured into a petri dish, left at room temperature to solidify, and cut into particles having a size of approximately 6 mm. Secondly, particles were coated with chitosan. Agar-gelatin particles were added into the chitosan solution and stirred. Particles were then collected by filtration and washed with phosphate buffered saline (PBS). As a result, particles prepared by the method described by Albadran H. et al. showed great potential for delivery of probiotics into the large intestine [69]. Coated particles showed the ability to withstand the environment of simulated gastric fluid (SGF) for 2 h of incubation and 3 h in simulated intestinal fluid (SIF). X-ray diffraction analysis showed a change in the physico-chemical properties of agar. This change caused by thermal treatment resulted in a strong and tight polymer network.

2.3. Nanoemulsion

The definition of a nanoemulsion system can vary as some sources describe that the droplet size is smaller than 500 nm whereas others claim the droplet size is up to 1000 nm [70][71][72][73][74]. The system is made of two immiscible liquids, as stated previously, which are stabilized using surfactant [75][76][77][78]. Nanoemulsions, as opposed to macro and microemulsions, have improved physico-chemical stability [79]. The immiscible liquids used most are water and oil [74][80]. There are two main types of nanoemulsion. The first type can be formed as oil-in-water (O/W); the second type is water-in-oil (W/O), depending on the dispersed liquid. Apart from these, they are also water-in-oil-in-water (W/O/W), and vice versa, and bi-continuous types [55][72][81][82]. Two main approaches are used for preparation, the so-called high and low-energy methods [83][84].
High-energy methods include high-pressure homogenization, microfluidization, and ultrasonication [74][85][86]. With these methods, mechanical energy is used to break large droplets. The main disadvantage of high-energy methods is cost, due to energy demand. Conversely, the advantages are good control of droplet size and possibility of choosing the formulation composition [85][87].
Low-energy methods include the phase inversion temperature and the emulsion inversion point [74][85]. Their principle uses internal chemical energy and formation of droplets with a change in, for example, temperature or chemical composition [86][87][88].
Particles are spheres with amorphous, lipophilic, and negatively charged surfaces [73][89]. A significant number of newly investigated drugs have problems with water solubility and thus their bioavailability is very low [78]. In the study by Dey et al. [90]., it was found that nanoemulsion enhances the absorption of lipids in the small intestine of rats more than conventional emulsion.
The group of Oh et al. [91]. developed a lecithin nano-liposol system loaded with astaxanthin (ASTA). Like other antioxidants, ASTA is susceptible to degradation and thus needs to be encapsulated. The method used for preparation was emulsion evaporation. Chloroform with different concentrations of ASTA was added to lecithin and mixed for 2 h. The final mixture was then added to deionized water and homogenized. Chloroform was removed by drying. The last steps were carried out using ultrasonication and purification by centrifuge. The best results were achieved with a loading of 15 wt% as it had a similar hydrodynamic diameter to that prior of loading. The diameter was around 140 nm ± 4 nm. The encapsulation efficiency for 15 wt% was 98.8% [91].
The publication by Tayeb and Sainsbury describes different uses of nanoemulsion in the pharmaceutical industry [78]. For the delivery of the drug, different ways of application can be used, i.e., nasal, ocular, oral, and parenteral [78][92]. The application of drugs through the skin can be challenging because of the protectiveness of skin layers. Nanoemulsion encapsulation can improve both bioavailability and penetration chance because of the nano-dimensions and low surface tension [78]. Quercetin as a well-known antioxidant with various health benefits, which can be encapsulated. It is necessary to do so because of its poor water solubility, skin absorption, and penetration ability, which are improved by nanoemulsion encapsulation [78][93][94]. Oral drug intake is specific because of the acidic environment and enzymes [95][96]. Encapsulation can decrease the impact of the environment and help with absorption of active substances [97].


  1. Trojanowska, A.; Nogalska, A.; Valls, R.G.; Giamberini, M.; Tylkowski, B. Technological solutions for encapsulation. Phys. Sci. Rev. 2017, 2.
  2. Huang, Y.; Stonehouse, A.; Abeykoon, C. Encapsulation methods for phase change materials—A critical review. Int. J. Heat Mass Transf. 2023, 200, 123458.
  3. Zabot, G.L.; Rodrigues, F.S.; Ody, L.P.; Tres, M.V.; Herrera, E.; Palacin, H.; Córdova-Ramos, J.S.; Best, I.; Olivera-Montenegro, L. Encapsulation of Bioactive Compounds for Food and Agricultural Applications. Polymers 2022, 14, 4194.
  4. Guía-García, J.L.; Charles-Rodríguez, A.V.; Reyes-Valdés, M.H.; Ramírez-Godina, F.; Robledo-Olivo, A.; García-Osuna, H.T.; Cerqueira, M.A.; Flores-López, M.L. Micro and nanoencapsulation of bioactive compounds for agri-food applications: A review. Ind. Crops Prod. 2022, 186, 115198.
  5. Sultana, M.; Chan, E.-S.; Pushpamalar, J.; Choo, W.S. Advances in extrusion-dripping encapsulation of probiotics and omega-3 rich oils. Trends Food Sci. Technol. 2022, 123, 69–86.
  6. Di Giorgio, L.; Salgado, P.R.; Mauri, A.N. Encapsulation of fish oil in soybean protein particles by emulsification and spray drying. Food Hydrocoll. 2019, 87, 891–901.
  7. Kandasamy, S.; Naveen, R. A review on the encapsulation of bioactive components using spray-drying and freeze-drying techniques. J. Food Process. Eng. 2022, 45, e14059.
  8. Bamidele, O.P.; Emmambux, M.N. Encapsulation of bioactive compounds by “extrusion” technologies: A review. Crit. Rev. Food Sci. Nutr. 2021, 61, 3100–3118.
  9. Devi, N.; Sarmah, M.; Khatun, B.; Maji, T.K. Encapsulation of active ingredients in polysaccharide–protein complex coacervates. Adv. Colloid Interface Sci. 2017, 239, 136–145.
  10. Grgić, J.; Šelo, G.; Planinić, M.; Tišma, M.; Bucić-Kojić, A. Role of the Encapsulation in Bioavailability of Phenolic Compounds. Antioxidants 2020, 9, 923.
  11. Dias, D.R.; Botrel, D.A.; Fernandes, R.V.D.B.; Borges, S.V. Encapsulation as a tool for bioprocessing of functional foods. Curr. Opin. Food Sci. 2017, 13, 31–37.
  12. Marcillo-Parra, V.; Tupuna-Yerovi, D.S.; González, Z.; Ruales, J. Encapsulation of bioactive compounds from fruit and vegetable by-products for food application—A review. Trends Food Sci. Technol. 2021, 116, 11–23.
  13. Food Encapsulation Market Size, Share, Global Trends, Forecasts to 2027. Available online: (accessed on 10 May 2023).
  14. Gao, J.; Jin, P.; Zhang, Y.; Dong, H.; Wang, R. Fast-responsive capsule based on two soluble components for self-healing concrete. Cem. Concr. Compos. 2022, 133, 104711.
  15. Timilsena, Y.P.; Haque, A.; Adhikari, B. Encapsulation in the Food Industry: A Brief Historical Overview to Recent Developments. Food Nutr. Sci. 2020, 11, 481–508.
  16. Mahmood, F.; Rehman, S.K.U.; Jameel, M.; Riaz, N.; Javed, M.F.; Salmi, A.; Awad, Y.A. Self-Healing Bio-Concrete Using Bacillus subtilis Encapsulated in Iron Oxide Nanoparticles. Materials 2022, 15, 7731.
  17. Li, H.; Wang, X. Preparation of microcapsules with IPDI monomer and isocyanate prepolymer as self-healing agent and their application in self-healing materials. Polymer 2022, 262, 125478.
  18. Papaioannou, S.; Amenta, M.; Kilikoglou, V.; Gournis, D.; Karatasios, I. Critical Aspects in the Development and Integration of Encapsulated Healing Agents in Cement and Concrete. J. Adv. Concr. Technol. 2021, 19, 301–320.
  19. Reda, M.A.; Chidiac, S.E. Performance of Capsules in Self-Healing Cementitious Material. Materials 2022, 15, 7302.
  20. Wang, H.; Zhou, Q. Evaluation and failure analysis of linseed oil encapsulated self-healing anticorrosive coating. Prog. Org. Coat. 2018, 118, 108–115.
  21. He, S.; Gao, Y.; Gong, X.; Wu, C.; Cen, H. Advance of design and application in self-healing anticorrosive coating: A review. J. Coat. Technol. Res. 2023, 20, 819–841.
  22. Ouarga, A.; Lebaz, N.; Tarhini, M.; Noukrati, H.; Barroug, A.; Elaissari, A.; Ben Youcef, H. Towards smart self-healing coatings: Advances in micro/nano-encapsulation processes as carriers for anti-corrosion coatings development. J. Mol. Liq. 2022, 354, 118862.
  23. Zhang, C.; Liu, R.; Chen, M.; Li, X.; Zhu, Z.; Yan, J. Effects of independently designed and prepared self-healing granules on self-healing efficiency for cement cracks. Constr. Build. Mater. 2022, 347, 128626.
  24. Reshma, V.; Mohanan, P. Quantum dots: Applications and safety consequences. J. Lumin. 2019, 205, 287–298.
  25. Lisi, F.; Sawayama, J.; Gautam, S.; Rubanov, S.; Duan, X.; Kirkwood, N. Re-Examination of the Polymer Encapsulation of Quantum Dots for Biological Applications. ACS Appl. Nano Mater. 2023, 6, 4046–4055.
  26. Ahmed, S.; Lahkar, S.; Doley, S.; Mohanta, D.; Dolui, S.K. A hierarchically porous MOF confined CsPbBr3 quantum dots: Fluorescence switching probe for detecting Cu (II) and melamine in food samples. J. Photochem. Photobiol. A Chem. 2023, 443, 114821.
  27. Prieto, C.; Talón, E.; Lagaron, J. Room temperature encapsulation of algae oil in water insoluble gluten extract. Food Hydrocoll. Health 2021, 1, 100022.
  28. Noor, A.; Al Murad, M.; Chitra, A.J.; Babu, S.N.; Govindarajan, S. Alginate based encapsulation of polyphenols of Piper betel leaves: Development, stability, bio-accessibility and biological activities. Food Biosci. 2022, 47, 101715.
  29. Wongverawattanakul, C.; Suklaew, P.O.; Chusak, C.; Adisakwattana, S.; Thilavech, T. Encapsulation of Mesona chinensis Benth Extract in Alginate Beads Enhances the Stability and Antioxidant Activity of Polyphenols under Simulated Gastrointestinal Digestion. Foods 2022, 11, 2378.
  30. Zhou, Y.; Xu, D.; Yu, H.; Han, J.; Liu, W.; Qu, D. Encapsulation of Salmonella phage SL01 in alginate/carrageenan micro-capsules as a delivery system and its application in vitro. Front. Microbiol. 2022, 13, 2718.
  31. Gupta, P.; Preet, S.; Ananya; Singh, N. Preparation of Thymus vulgaris (L.) essential oil nanoemulsion and its chitosan encapsulation for controlling mosquito vectors. Sci. Rep. 2022, 12, 4335.
  32. Chomchoey, S.; Klongdee, S.; Peanparkdee, M.; Klinkesorn, U. Fabrication and characterization of nanoemulsions for encapsulation and delivery of vitexin: Antioxidant activity, storage stability and in vitro digestibility. J. Sci. Food Agric. 2023, 103, 2532–2543.
  33. Opustilová, K.; Lapčíková, B.; Lapčík, L.; Gautam, S.; Valenta, T.; Li, P. Physico-Chemical Study of Curcumin and Its Application in O/W/O Multiple Emulsion. Foods 2023, 12, 1394.
  34. Paul, A.; Dutta, A.; Kundu, A.; Saha, S. Resin Assisted Purification of Anthocyanins and Their Encapsulation. J. Chem. Educ. 2023, 100, 885–892.
  35. Savoldi, T.E.; Scheufele, F.B.; Drunkler, D.A.; da Silva, G.J.; de Lima, J.D.; Maestre, K.L.; Triques, C.C.; da Silva, E.A.; Fiorese, M.L. Microencapsulation of Saccharomyces boulardii using vegan and vegetarian wall materials. J. Food Process. Preserv. 2022, 46, e16596.
  36. Blagojević, B.; Četojević-Simin, D.; Djurić, S.; Lazzara, G.; Milioto, S.; Agić, D.; Vasile, B.S.; Popović, B.M. Anthocyanins and phenolic acids from Prunus spinosa L. encapsulation in halloysite and maltodextrin based carriers. Appl. Clay Sci. 2022, 222, 106489.
  37. Tavares, L.; Smaoui, S.; Lima, P.S.; de Oliveira, M.M.; Santos, L. Propolis: Encapsulation and application in the food and pharmaceutical industries. Trends Food Sci. Technol. 2022, 127, 169–180.
  38. Shakoury, N.; Aliyari, M.A.; Salami, M.; Emam-Djomeh, Z.; Vardhanabhuti, B.; Moosavi-Movahedi, A.A. Encapsulation of propolis extract in whey protein nanoparticles. LWT 2022, 158, 113138.
  39. Villar, M.A.L.; Vidallon, M.L.P.; Rodriguez, E.B. Nanostructured lipid carrier for bioactive rice bran gamma-oryzanol. Food Biosci. 2022, 50, 102064.
  40. Enayati, M.; Madarshahian, S.; Yan, B.; Ufheil, G.; Abbaspourrad, A. Granulation and encapsulation of N-Acetylcysteine (NAC) by internal phase separation. Food Hydrocoll. 2022, 130, 107699.
  41. Arroyo-Maya, I.J.; McClements, D.J. Biopolymer nanoparticles as potential delivery systems for anthocyanins: Fabrication and properties. Food Res. Int. 2015, 69, 1–8.
  42. Wu, Y.; Wang, X.; Yin, Z.; Dong, J. Geotrichum candidum arthrospore cell wall particles as a novel carrier for curcumin encapsulation. Food Chem. 2023, 404, 134308.
  43. Thauer, E.; Shi, X.; Zhang, S.; Chen, X.; Deeg, L.; Klingeler, R.; Wenelska, K.; Mijowska, E. Mn3O4 encapsulated in hollow carbon spheres coated by graphene layer for enhanced magnetization and lithium-ion batteries performance. Energy 2021, 217, 119399.
  44. Ganganboina, A.B.; Chowdhury, A.D.; Khoris, I.M.; Doong, R.-A.; Li, T.-C.; Hara, T.; Abe, F.; Suzuki, T.; Park, E.Y. Hollow magnetic-fluorescent nanoparticles for dual-modality virus detection. Biosens. Bioelectron. 2020, 170, 112680.
  45. Surynek, M.; Spanhel, L.; Lapcik, L.; Mrazek, J. Tuning the photocatalytic properties of sol–gel-derived single, coupled, and alloyed ZnO–TiO2 nanoparticles. Res. Chem. Intermed. 2019, 45, 4193–4204.
  46. Choińska-Pulit, A.; Mituła, P.; Śliwka, P.; Łaba, W.; Skaradzińska, A. Bacteriophage encapsulation: Trends and potential applications. Trends Food Sci. Technol. 2015, 45, 212–221.
  47. Bacteriophage | Definition, Life Cycle, & Research | Britannica. Available online: (accessed on 3 January 2022).
  48. Rahimzadeh, G.; Saeedi, M.; Moosazadeh, M.; Hashemi, S.M.H.; Babaei, A.; Rezai, M.S.; Kamel, K.; Asare-Addo, K.; Nokhodchi, A. Encapsulation of bacteriophage cocktail into chitosan for the treatment of bacterial diarrhea. Sci. Rep. 2021, 11, 15603.
  49. Huff, W.; Huff, G.; Rath, N.; Donoghue, A. Method of administration affects the ability of bacteriophage to prevent colibacillosis in 1-day-old broiler chickens. Poult. Sci. 2013, 92, 930–934.
  50. Kaikabo, A.A.; Mohammed, A.S.; Abas, F. Chitosan Nanoparticles as Carriers for the Delivery of ΦKAZ14 Bacteriophage for Oral Biological Control of Colibacillosis in Chickens. Molecules 2016, 21, 256.
  51. Camelo-Silva, C.; Verruck, S.; Ambrosi, A.; Di Luccio, M. Innovation and Trends in Probiotic Microencapsulation by Emulsification Techniques. Food Eng. Rev. 2022, 14, 462–490.
  52. Fujiu, K.B.; Kobayashi, I.; Uemura, K.; Nakajima, M. Temperature effect on microchannel oil-in-water emulsification. Microfluid. Nanofluid. 2011, 10, 773–783.
  53. McClements, D.J. Edible nanoemulsions: Fabrication, properties, and functional performance. Soft Matter 2010, 7, 2297–2316.
  54. Ozkan, G.; Kostka, T.; Esatbeyoglu, T.; Capanoglu, E. Effects of Lipid-Based Encapsulation on the Bioaccessibility and Bioavailability of Phenolic Compounds. Molecules 2020, 25, 5545.
  55. Lu, W.; Kelly, A.; Miao, S. Emulsion-based encapsulation and delivery systems for polyphenols. Trends Food Sci. Technol. 2016, 47, 1–9.
  56. Calabrese, V.; Courtenay, J.C.; Edler, K.J.; Scott, J.L. Pickering emulsions stabilized by naturally derived or biodegradable particles. Curr. Opin. Green Sustain. Chem. 2018, 12, 83–90.
  57. Comunian, T.A.; Anthero, A.G.D.S.; Bezerra, E.O.; Moraes, I.C.F.; Hubinger, M.D. Encapsulation of Pomegranate Seed Oil by Emulsification Followed by Spray Drying: Evaluation of Different Biopolymers and Their Effect on Particle Properties. Food Bioprocess Technol. 2019, 13, 53–66.
  58. Dinkgreve, M.; Velikov, K.P.; Bonn, D. Stability of LAPONITE®-stabilized high internal phase Pickering emulsions under shear. Phys. Chem. Chem. Phys. 2016, 18, 22973–22977.
  59. Ganley, W.J.; van Duijneveldt, J.S. Controlling the Rheology of Montmorillonite Stabilized Oil-in-Water Emulsions. Langmuir 2017, 33, 1679–1686.
  60. Gbassi, G.K.; Vandamme, T. Probiotic Encapsulation Technology: From Microencapsulation to Release into the Gut. Pharmaceutics 2012, 4, 149–163.
  61. Dini, C.; Islan, G.A.; de Urraza, P.J.; Castro, G.R. Novel Biopolymer Matrices for Microencapsulation of Phages: Enhanced Protection Against Acidity and Protease Activity. Macromol. Biosci. 2012, 12, 1200–1208.
  62. Kim, S.-G.; Giri, S.S.; Jo, S.-J.; Kang, J.-W.; Lee, S.-B.; Jung, W.-J.; Lee, Y.-M.; Kim, H.-J.; Kim, J.-H.; Park, S.-C. Prolongation of Fate of Bacteriophages In Vivo by Polylactic-Co-Glycolic-Acid/Alginate-Composite Encapsulation. Antibiotics 2022, 11, 1264.
  63. Koh, W.Y.; Lim, X.X.; Tan, T.-C.; Kobun, R.; Rasti, B. Encapsulated Probiotics: Potential Techniques and Coating Materials for Non-Dairy Food Applications. Appl. Sci. 2022, 12, 10005.
  64. Safiah Sabrina Hassan, Intan Nabihah Ahmad Fadzil, Anida Yusoff, and Khalilah Abdul Khalil. A Review on Microencap-sulation in Improving Probiotic Stability for Beverages Application. 2020. Available online: (accessed on 23 January 2023).
  65. Liliana, S.C.; Vladimir, V.C.; Serna-Cock, L.; Vallejo-Castillo, V. Probiotic encapsulation. Afr. J. Microbiol. Res. 2013, 7, 4743–4753.
  66. Tang, Z.; Huang, X.; Baxi, S.; Chambers, J.R.; Sabour, P.M.; Wang, Q. Whey protein improves survival and release characteristics of bacteriophage Felix O1 encapsulated in alginate microspheres. Food Res. Int. 2013, 52, 460–466.
  67. Yin, H.; Li, J.; Huang, H.; Wang, Y.; Qian, X.; Ren, J.; Xue, F.; Dai, J.; Tang, F. Microencapsulated phages show prolonged stability in gastrointestinal environments and high therapeutic efficiency to treat Escherichia coli O157:H7 infection. Vet. Res. 2021, 52, 118.
  68. Savic, I.M.; Gajic, I.M.S.; Milovanovic, M.G.; Zerajic, S.; Gajic, D.G. Optimization of Ultrasound-Assisted Extraction and Encapsulation of Antioxidants from Orange Peels in Alginate-Chitosan Microparticles. Antioxidants 2022, 11, 297.
  69. Albadran, H.A.; Monteagudo-Mera, A.; Khutoryanskiy, V.V.; Charalampopoulos, D. Development of chitosan-coated agar-gelatin particles for probiotic delivery and targeted release in the gastrointestinal tract. Appl. Microbiol. Biotechnol. 2020, 104, 5749–5757.
  70. Gupta, A. Nanoemulsions. In Nanoparticles for Biomedical Applications: Fundamental Concepts, Biological Interactions and Clinical Applications; Elsevier Science: Amsterdam, The Netherlands, 2020; Available online: (accessed on 10 January 2023).
  71. Mandal, A.; Bera, A.; Ojha, K.; Kumar, T. Characterization of Surfactant Stabilized Nanoemulsion and Its Use in Enhanced Oil Recovery. In Proceedings of the SPE International Oilfield Nanotechnology Conference and Exhibition, Noordwijk, The Netherlands, 12–14 June 2012.
  72. Rodríguez, J.; Martín, M.J.; Ruiz, M.A.; Clares, B. Current encapsulation strategies for bioactive oils: From alimentary to pharmaceutical perspectives. Food Res. Int. 2016, 83, 41–59.
  73. Jaiswal, M.; Dudhe, R.; Sharma, P.K. Nanoemulsion: An advanced mode of drug delivery system. 3 Biotech 2014, 5, 123–127.
  74. Oprea, I.; Fărcaș, A.C.; Leopold, L.F.; Diaconeasa, Z.; Coman, C.; Socaci, S.A. Nano-Encapsulation of Citrus Essential Oils: Methods and Applications of Interest for the Food Sector. Polymers 2022, 14, 4505.
  75. Singh, Y.; Meher, J.G.; Raval, K.; Khan, F.A.; Chaurasia, M.; Jain, N.K.; Chourasia, M.K. Nanoemulsion: Concepts, development and applications in drug delivery. J. Control. Release 2017, 252, 28–49.
  76. McClements, D.J.; Das, A.K.; Dhar, P.; Nanda, P.K.; Chatterjee, N. Nanoemulsion-Based Technologies for Delivering Natural Plant-Based Antimicrobials in Foods. Front. Sustain. Food Syst. 2021, 5, 643208.
  77. Kale, S.N.; Deore, S.L. Emulsion Micro Emulsion and Nano Emulsion: A Review. Syst. Rev. Pharm. 2017, 8, 39–47.
  78. Tayeb, H.H.; Sainsbury, F. Nanoemulsions in drug delivery: Formulation to medical application. Nanomedicine 2018, 13, 2507–2525.
  79. Sharma, S.; Cheng, S.-F.; Bhattacharya, B.; Chakkaravarthi, S. Efficacy of free and encapsulated natural antioxidants in oxidative stability of edible oil: Special emphasis on nanoemulsion-based encapsulation. Trends Food Sci. Technol. 2019, 91, 305–318.
  80. Salvia-Trujillo, L.; Martín-Belloso, O.; McClements, D.J. Excipient Nanoemulsions for Improving Oral Bioavailability of Bioactives. Nanomaterials 2016, 6, 17.
  81. Sneha, K.; Kumar, A. Nanoemulsions: Techniques for the preparation and the recent advances in their food applications. Innov. Food Sci. Emerg. Technol. 2022, 76, 102914.
  82. Liu, Q.; Huang, H.; Chen, H.; Lin, J.; Wang, Q. Food-Grade Nanoemulsions: Preparation, Stability and Application in Encapsulation of Bioactive Compounds. Molecules 2019, 24, 4242.
  83. Modarres-Gheisari, S.M.M.; Gavagsaz-Ghoachani, R.; Malaki, M.; Safarpour, P.; Zandi, M. Ultrasonic nano-emulsification—A review. Ultrason. Sonochem. 2019, 52, 88–105.
  84. Salem, M.A.; Ezzat, S.M. Nanoemulsions in Food Industry. In Some New Aspects of Colloidal Systems in Foods; IntechOpen: London, UK, 2019.
  85. Kumar, M.; Bishnoi, R.S.; Shukla, A.K.; Jain, C.P. Techniques for Formulation of Nanoemulsion Drug Delivery System: A Review. Prev. Nutr. Food Sci. 2019, 24, 225–234.
  86. Gonçalves, A.; Nikmaram, N.; Roohinejad, S.; Estevinho, B.N.; Rocha, F.; Greiner, R.; McClements, D.J. Production, properties, and applications of solid self-emulsifying delivery systems (S-SEDS) in the food and pharmaceutical industries. Colloids Surfaces A Physicochem. Eng. Asp. 2018, 538, 108–126.
  87. Jasmina, H.; Džana, O.; Alisa, E.; Edina, V.; Ognjenka, R. Preparation of Nanoemulsions by high-energy and lowenergy emulsification methods. In CMBEBIH 2017: Proceedings of the International Conference on Medical and Biological Engineering 2017; Springer Verlag: Berlin/Heidelberg, Germany, 2017; Volume 62, pp. 317–322.
  88. Solans, C.; Solé, I. Nano-emulsions: Formation by low-energy methods. Curr. Opin. Colloid Interface Sci. 2012, 17, 246–254.
  89. Moghaddasi, F.; Housaindokht, M.R.; Darroudi, M.; Bozorgmehr, M.R.; Sadeghi, A. Synthesis of nano curcumin using black pepper oil by O/W Nanoemulsion Technique and investigation of their biological activities. LWT 2018, 92, 92–100.
  90. Dey, T.K.; Ghosh, S.; Ghosh, M.; Koley, H.; Dhar, P. Comparative study of gastrointestinal absorption of EPA & DHA rich fish oil from nano and conventional emulsion formulation in rats. Food Res. Int. 2012, 49, 72–79.
  91. Oh, H.; Lee, J.S.; Sung, D.; Lim, J.M.; Choi, W.I. Potential Antioxidant and Wound Healing Effect of Nano-Liposol with High Loading Amount of Astaxanthin. Int. J. Nanomed. 2020, 15, 9231–9240.
  92. Bonferoni, M.C.; Rossi, S.; Sandri, G.; Ferrari, F.; Gavini, E.; Rassu, G.; Giunchedi, P. Nanoemulsions for “Nose-to-Brain” Drug Delivery. Pharmaceutics 2019, 11, 84.
  93. Ulusoy, H.G.; Sanlier, N. A minireview of quercetin: From its metabolism to possible mechanisms of its biological activities. Crit. Rev. Food Sci. Nutr. 2020, 60, 3290–3303.
  94. Lu, B.; Huang, Y.; Chen, Z.; Ye, J.; Xu, H.; Chen, W.; Long, X. Niosomal Nanocarriers for Enhanced Skin Delivery of Quercetin with Functions of Anti-Tyrosinase and Antioxidant. Molecules 2019, 24, 2322.
  95. Basha, S.K.; Muzammil, M.S.; Dhandayuthabani, R.; Kumari, V.S. Development of nanoemulsion of Alginate/Aloe vera for oral delivery of insulin. Mater. Today Proc. 2021, 36, 357–363.
  96. Sánchez-Navarro, M.; Garcia, J.; Giralt, E.; Teixidó, M. Using peptides to increase transport across the intestinal barrier. Adv. Drug Deliv. Rev. 2016, 106, 355–366.
  97. Meng, Q.; Long, P.; Zhou, J.; Ho, C.-T.; Zou, X.; Chen, B.; Zhang, L. Improved absorption of β-carotene by encapsulation in an oil-in-water nanoemulsion containing tea polyphenols in the aqueous phase. Food Res. Int. 2019, 116, 731–736.
Contributors MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to : , , ,
View Times: 327
Revisions: 2 times (View History)
Update Date: 16 Jun 2023
Video Production Service