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HandWiki. RESOLFT. Encyclopedia. Available online: https://encyclopedia.pub/entry/36543 (accessed on 21 September 2026).
HandWiki. RESOLFT. Encyclopedia. Available at: https://encyclopedia.pub/entry/36543. Accessed September 21, 2026.
HandWiki. "RESOLFT" Encyclopedia, https://encyclopedia.pub/entry/36543 (accessed September 21, 2026).
HandWiki. (2022, November 25). RESOLFT. In Encyclopedia. https://encyclopedia.pub/entry/36543
HandWiki. "RESOLFT." Encyclopedia. Web. 25 November, 2022.
RESOLFT
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The content is sourced from: https://handwiki.org/wiki/Physics:RESOLFT

RESOLFT, an acronym for REversible Saturable OpticaL Fluorescence Transitions, denotes a group of optical fluorescence microscopy techniques with very high resolution. Using standard far field visible light optics a resolution far below the diffraction limit down to molecular scales can be obtained. With conventional microscopy techniques, it is not possible to distinguish features that are located at distances less than about half the wavelength used (i.e. about 200 nm for visible light). This diffraction limit is based on the wave nature of light. In conventional microscopes the limit is determined by the used wavelength and the numerical aperture of the optical system. The RESOLFT concept surmounts this limit by temporarily switching the molecules to a state in which they cannot send a (fluorescence-) signal upon illumination. This concept is different from for example electron microscopy where instead the used wavelength is much smaller.

fluorescence microscopy electron microscopy fluorescence

References

  1. Stefan W. Hell; Jan Wichmann (1994). "Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy". Optics Letters 19 (11): 780–2. doi:10.1364/OL.19.000780. PMID 19844443. Bibcode: 1994OptL...19..780H. http://www.opticsinfobase.org/ol/viewmedia.cfm?URI=ol-19-11-780&seq=0. 
  2. Thomas A. Klar; Stefan W. Hell (1999). "Subdiffraction resolution in far-field fluorescence microscopy". Optics Letters 24 (14): 954–956. doi:10.1364/OL.24.000954. PMID 18073907. Bibcode: 1999OptL...24..954K. http://www.opticsinfobase.org/ol/viewmedia.cfm?uri=ol-24-14-954&seq=0. 
  3. See also Confocal laser scanning microscopy.
  4. Stefan W. Hell (2004). "Strategy for far-field optical imaging and writing without diffraction limit". Physics Letters A 326 (1–2): 140–145. doi:10.1016/j.physleta.2004.03.082. Bibcode: 2004PhLA..326..140H.  https://dx.doi.org/10.1016%2Fj.physleta.2004.03.082
  5. Rainer Heintzmann; Thomas M. Jovin; Christoph Cremer (2002). "Saturated patterned excitation microscopy a concept for optical resolution improvement". Journal of the Optical Society of America A 19 (8): 1599–2109. doi:10.1364/JOSAA.19.001599. PMID 12152701. Bibcode: 2002JOSAA..19.1599H. http://www.opticsinfobase.org/josaa/viewmedia.cfm?uri=josaa-19-8-1599&seq=0. 
  6. Mats G. L. Gustafsson (2005). "Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution". Proceedings of the National Academy of Sciences of the United States of America 102 (37): 13081–6. doi:10.1073/pnas.0406877102. PMID 16141335. Bibcode: 2005PNAS..10213081G.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1201569
  7. Michael Hofmann; Christian Eggeling; Stefan Jakobs; Stefan W. Hell (2005). "Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins". Proceedings of the National Academy of Sciences of the United States of America 102 (49): 17565–9. doi:10.1073/pnas.0506010102. PMID 16314572. Bibcode: 2005PNAS..10217565H.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1308899
  8. Ulrike Böhm; Stefan W. Hell; Roman Schmidt (2016). "4Pi-RESOLFT nanoscopy". Nature Communications 7 (10504): 1–8. doi:10.1038/ncomms10504. PMID 26833381. Bibcode: 2016NatCo...710504B.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4740410
  9. Mariano Bossi; Jonas Fölling; Marcus Dyba; Volker Westphal; Stefan W. Hell (2006). "Breaking the diffraction resolution barrier in far-field microscopy by molecular optical bistability". New Journal of Physics 8 (11): 275. doi:10.1088/1367-2630/8/11/275. Bibcode: 2006NJPh....8..275B.  https://dx.doi.org/10.1088%2F1367-2630%2F8%2F11%2F275
  10. Jiwoong Kwon; Jihee Hwang; Jaewan Park; Gi Rim Han; Kyu Young Han; Seong Keun Kim (2015). "RESOLFT nanoscopy with photoswitchable organic fluorophores". Scientific Reports 5: 17804. doi:10.1038/srep17804. PMID 26639557. Bibcode: 2015NatSR...517804K.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4671063
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