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HandWiki. Read. Encyclopedia. Available online: https://encyclopedia.pub/entry/36299 (accessed on 26 September 2026).
HandWiki. Read. Encyclopedia. Available at: https://encyclopedia.pub/entry/36299. Accessed September 26, 2026.
HandWiki. "Read" Encyclopedia, https://encyclopedia.pub/entry/36299 (accessed September 26, 2026).
HandWiki. (2022, November 24). Read. In Encyclopedia. https://encyclopedia.pub/entry/36299
HandWiki. "Read." Encyclopedia. Web. 24 November, 2022.
Read
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The content is sourced from: https://handwiki.org/wiki/Biology:Read

In DNA sequencing, a read is an inferred sequence of base pairs (or base pair probabilities) corresponding to all or part of a single DNA fragment. A typical sequencing experiment involves fragmentation of the genome into millions of molecules, which are size-selected and ligated to adapters. The set of fragments is referred to as a sequencing library, which is sequenced to produce a set of reads.

base pair fragmentation DNA sequencing

References

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  2. Junemann, Sebastian (2013). "Updating benchtop sequencing performance comparison". Nature Biotechnology 31 (4): 294–296. doi:10.1038/nbt.2522. PMID 23563421.  https://dx.doi.org/10.1038%2Fnbt.2522
  3. Quail, Michael A. (2012). "A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers". BMC Genomics 13 (1): 341. doi:10.1186/1471-2164-13-341. PMID 22827831.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3431227
  4. Chhangawala, Sagar; Rudy, Gabe; Mason, Christopher E.; Rosenfeld, Jeffrey A. (23 June 2015). "The impact of read length on quantification of differentially expressed genes and splice junction detection". Genome Biology 16 (1): 131. doi:10.1186/s13059-015-0697-y. PMID 26100517.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4531809
  5. Chaisson, Mark J.P. (2015). "Genetic variation and the de novo assembly of human genomes". Nature Reviews Genetics 16 (11): 627–640. doi:10.1038/nrg3933. PMID 26442640.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4745987
  6. Conesa, Ana; Madrigal, Pedro; Tarazona, Sonia; Gomez-Cabrero, David; Cervera, Alejandra; McPherson, Andrew; Szcześniak, Michał Wojciech; Gaffney, Daniel J. et al. (26 January 2016). "A survey of best practices for RNA-seq data analysis". Genome Biology 17 (1): 13. doi:10.1186/s13059-016-0881-8. PMID 26813401.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4728800
  7. Giani, Alice Maria; Gallo, Guido Roberto; Gianfranceschi, Luca; Formenti, Giulio (2020). "Long walk to genomics: History and current approaches to genome sequencing and assembly". Computational and Structural Biotechnology Journal 18: 9–19. doi:10.1016/j.csbj.2019.11.002. PMID 31890139.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=6926122
  8. Qiang-long, Zhu; Shi, Liu; Peng, Gao; Fei-shi, Luan (1 September 2014). "High-throughput Sequencing Technology and Its Application". Journal of Northeast Agricultural University (English Edition) 21 (3): 84–96. doi:10.1016/S1006-8104(14)60073-8.  https://dx.doi.org/10.1016%2FS1006-8104%2814%2960073-8
  9. Chaisson, M.; Pevzner, P.; Tang, H. (1 September 2004). "Fragment assembly with short reads". Bioinformatics 20 (13): 2067–2074. doi:10.1093/bioinformatics/bth205.  https://dx.doi.org/10.1093%2Fbioinformatics%2Fbth205
  10. Kraft, Florian; Kurth, Ingo (16 July 2019). "Long-read sequencing in human genetics". Medizinische Genetik 31 (2): 198–204. doi:10.1007/s11825-019-0249-z.  https://dx.doi.org/10.1007%2Fs11825-019-0249-z
  11. Sung, Wing-Kin (2017). Algorithms for next-generation sequencing. Boca Raton. ISBN 978-1466565500. 
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  13. Adewale, Boluwatife A. (26 November 2020). "Will long-read sequencing technologies replace short-read sequencing technologies in the next 10 years?". African Journal of Laboratory Medicine 9 (1): 5. doi:10.4102/ajlm.v9i1.1340. PMID 33354530.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=7736650
  14. "DNA Sequencing Costs: Data" (in en). https://www.genome.gov/about-genomics/fact-sheets/DNA-Sequencing-Costs-Data. 
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  21. Simpson, Jared T; Workman, Rachael E; Zuzarte, P C; David, Matei; Dursi, L J; Timp, Winston (April 2017). "Detecting DNA cytosine methylation using nanopore sequencing". Nature Methods 14 (4): 407–410. doi:10.1038/nmeth.4184. PMID 28218898.  https://dx.doi.org/10.1038%2Fnmeth.4184
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