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Reference Point Indentation (RPI) refers to a specialized form of indentation testing. RPI utilizes a unique method of measurement by establishing a relative reference point at the location of measurement. This unique capability makes it possible to measure materials that are in motion, oddly shaped, visco-elastic, or that may be coated or covered by another, softer material. Unlike traditional indentation testing, RPI testing uses the location of measurement as the relative displacement reference position. Indentation itself is perhaps the most commonly applied means of testing the mechanical properties of materials. The technique has its origins in the Mohs scale of mineral hardness, in which materials are ranked according to what they can scratch and are, in turn, scratched by. The characterization of solids in this way takes place on an essentially discrete scale, so much effort has been expended in order to develop techniques for evaluating material hardness over a continuous range. Hence, the adoption of the Meyer, Knoop, Brinell, Rockwell, and Vickers hardness tests. More recently (ca. 1975), nanoindentation techniques have been established as the primary tool for investigating the hardness of small volumes of material. However, even more recently (ca. 2006), interest in measuring functional roles of biomaterials drove the development of the Reference Point Indentation technique. New research in field such as biomaterials has led scientists to begin considering materials as complex systems that behave differently than the constituent parts. For example, materials like bone are hierarchical and made of many components including calcium, collagen, water, and non-collagenous proteins. Each of these components has unique material properties. When combined to form bone, the function of the tissue is different than any one constituent. Understanding this mechanical system is becoming a new field of research called Materiomics. RPI specifically aims to aid materiomics researchers understand the functional capabilities of these types of materials at a relevant length-scale.