Submitted Successfully!
To reward your contribution, here is a gift for you: A free trial for our video production service.
Thank you for your contribution! You can also upload a video entry or images related to this topic.
Version Summary Created by Modification Content Size Created at Operation
1 -- 1539 2022-10-31 10:31:49 |
2 format correct -1 word(s) 1538 2022-11-01 02:10:39 |

Video Upload Options

Do you have a full video?

Confirm

Are you sure to Delete?
Cite
If you have any further questions, please contact Encyclopedia Editorial Office.
Naskar, A.;  Kim, K. Photo-Stimuli-Responsive Antibacterial Applications of CuS and Its Nanocomposites. Encyclopedia. Available online: https://encyclopedia.pub/entry/32065 (accessed on 17 April 2024).
Naskar A,  Kim K. Photo-Stimuli-Responsive Antibacterial Applications of CuS and Its Nanocomposites. Encyclopedia. Available at: https://encyclopedia.pub/entry/32065. Accessed April 17, 2024.
Naskar, Atanu, Kwang-Sun Kim. "Photo-Stimuli-Responsive Antibacterial Applications of CuS and Its Nanocomposites" Encyclopedia, https://encyclopedia.pub/entry/32065 (accessed April 17, 2024).
Naskar, A., & Kim, K. (2022, October 31). Photo-Stimuli-Responsive Antibacterial Applications of CuS and Its Nanocomposites. In Encyclopedia. https://encyclopedia.pub/entry/32065
Naskar, Atanu and Kwang-Sun Kim. "Photo-Stimuli-Responsive Antibacterial Applications of CuS and Its Nanocomposites." Encyclopedia. Web. 31 October, 2022.
Photo-Stimuli-Responsive Antibacterial Applications of CuS and Its Nanocomposites
Edit

Photo-stimuli-responsive therapeutic nanomaterials have gained widespread attention as frontline materials for biomedical applications. The photoactivation strategies are classified as single-modality (based on either reactive oxygen species (ROS)-based photodynamic therapy (PDT), hyperthermia-based photothermal therapy (PTT)), or dual-modality (which combines PDT and PTT). CuS has excellent photoactivated antibacterial properties and shows promising antibacterial activity when irradiated in the so-called biological window (i.e., NIR region).

photo-stimuli-responsive nanomaterial CuS photodynamic therapy photothermal therapy

1. CuS Nanomaterials

CuS nanomaterials exhibit inherent photoactivated antibacterial potential, especially in the NIR region. For example, Qiao et al. [1], developed ultrasmall copper sulfide (covellite) nanodots (CuS NDs), which showed effectiveness in the treatment of multidrug-resistant bacteria-infected chronic non-healing wounds. The dual functional mode of the CuS nanosystem enabled eradication of extended-spectrum β-lactamase (ESBL) E. coli and methicillin-resistant S. aureus (MRSA) in both in vitro and in vivo studies, while simultaneously expediting wound healing. The NIR laser (808 nm, 2.5 W·cm−2)-mediated photothermal effect and remote control of copper-ion (Cu2+) release were the main origins of the dual-mode activity. Ren et al. [2] utilized CuS nanoparticles (NPs) to prepare a photothermal antibacterial silk fabric. A chitosan quaternary ammonium salt (QCS) was used as a template. The antibacterial efficiency of CuS NP-deposited silk fabric with QCS as a template (SF/QCS/CuS) resulted in 99.99% bacterial death of S. aureus and E. coli within 5 min of irradiation (400 mW·cm−2). The nanocomposite also showed good UV-resistance and superb light-to-heat conversion efficiency. Even after 10 washes, the antibacterial activity of the nanocomposite remained at a high level, demonstrating excellent bonding between the photothermal nanomaterials. In a similar experiment, CuS NPs were coated onto glass slides, and the antibacterial properties were investigated [3]. The CuS NP–glass samples showed good stability and an admirable photothermal effect when irradiated with NIR light. The samples were also effective for the controlled release of copper in water. Hence, the antibacterial activity of the CuS NP-glass samples against E. coli and S. aureus was attributed to the dual mechanism of hyperthermia-induced NIR laser irradiation and the slow and sustained copper release. As discussed earlier, CuS has shown morphology-dependent photodynamic and photothermal antibacterial activities [4].

2. CuS with 2D Nanomaterials

In recent times, 2D nanomaterials have revolutionized research due to their unique physiochemical properties, non-toxicity, and excellent utility in various biomedical applications, including antibacterial treatments [5][6][7]. After the immense success of graphene [8][9], researchers have been exploring different 2D nanomaterials as antibacterial agents, namely graphitic carbon nitride (g-C3N4) nanosheets [10][11], titanium carbide (Mxene) [12], boron nitride (BN) [13], transition metal dichalcogenides [e.g., molybdenum disulfide (MoS2)] [14], BP [15][16][17][18], and others. The basic advantage of using 2D nanomaterials with CuS is the synergetic improvement in the antibacterial activity, with less chance of toxicity, due to the biocompatibility of 2D materials.
In this respect, Lv et al. [19] demonstrated the importance of CuS nanocomposites with graphene oxide (GO) for photothermal antibacterial applications by utilizing visible light. A simple one-pot hydrothermal method was utilized to synthesize flower-like CuS anchored on GO sheets. Due to the morphology-dependent high surface area of CuS and defects in GO, the nanocomposite showed excellent photocatalytic performance. The nanocomposite showed excellent antibacterial activity against E. coli and S. aureus. This antibacterial activity was attributed to the excellent photocatalytic activity of the nanocomposite, coupled with the visible-light-induced (0.2 W·cm−2) photothermal response and release of Cu ions. The nanomaterial was also biocompatible with L929, a murine fibroblast cell line. Another 2D nanomaterial, graphitic carbon nitride (g-C3N4) nanosheets [20], were utilized with CuS nanomaterials for the photothermal eradication of bacteria. In that study, a simple hydrothermal process was again utilized to fabricate CuS nanomaterial-decorated graphitic carbon nitride (g-C3N4) nanosheets. Thereafter, polyethylene glycol (PEG) was capped with the nanocomposite to make it more biocompatible. The PEG-CuS@g-C3N4 nanocomposite was effective as an antibacterial agent, killing up to 99% of both E. coli. and S. aureus in a 200 µg·mL−1 suspension within 20 min of NIR irradiation (808 nm, 2.5 W·cm−2). The biocompatibility of the nanocomposite was also verified using mouse skin fibroblast NIH-3T3 cell lines. Ti3C2Tx Mxene was also used with CuS to achieve PDT- and PTT-mediated multimodal activity against E. coli and S. aureus, with bactericidal rates of 99.6 and 99.1%, respectively [21]. Ti3C2Tx@CuS composites were synthesized by a simple hydrothermal method, where the CuS NPs were distributed on the surface of Ti3C2Tx, affording synergistic effects. ROS generation, which eventually facilitated destruction of the bacterial cells, was attributed to the formation of the Ti3C2Tx@CuS heterojunction, which promoted the separation of electrons and holes, leading to improved electron transport efficiency. Furthermore, the nanocomposite had a stronger photothermal effect than the individual components, confirming its synergistic action. Recently, the researchers reported a BP-based CuS nanoplatform (CB) with photothermal activity, demonstrating antibacterial activity against environmental bacterial pathogens [16]. In the work, a low-temperature solution synthesis method was utilized to prepare CuS nanomaterials, which were then immobilized on BP nanosheets. The synthesized CB nanocomposites showed excellent antibacterial activity against P. aeruginosa and S. aureus, and also showed good activity against the MDR strains of these bacteria after irradiation with NIR light (808 nm, 2.5 W·cm−2). The CB nanocomposite also showed exceptional ROS generation ability under NIR irradiation relative to the non-NIR-irradiated sample.

3. CuS with Polymers

CuS nanomaterials were also utilized with different polymers to achieve synergistic photoactivated antibacterial activity. Dai et al. [22] synthesized a collection of poly(5-(2-ethyl acrylate)-4-methylthiazole-g-butyl)/copper sulfide nanoclusters (PATAC4@CuS), which were applied to levofloxacin-resistant Gram-negative and Gram-positive bacteria for efficient capture and effective ablation by NIR laser irradiation. The nanoclusters not only showed excellent photothermal activity under NIR irradiation, but also demonstrated admirable photodynamic properties. PATAC4@CuS nanoclusters showed excellent antibacterial activity against B. amyloliquefaciens, E. coli, P. aeruginosa, and levofloxacin-resistant S. aureus at 5.5 μg·mL−1 under NIR laser irradiation (980 nm, 1.5 W·cm−2, 5 min). The heat and ROS generated from the NIR-irradiated PATAC4@CuS nanoclusters were stated as the reasons for the effective elimination and prevention of regrowth of the bacteria. The nanocluster also showed wound healing potential in bacteria-infected rat wounds without nonspecific harm to normal tissue. In another similar experiment, Wang et al. [23] introduced a thiol-terminated, alkyl-containing short-chain poly(ethylene glycol) (HS-(CH2)11-(OCH2CH2)6-OH, abbreviated as MUH, onto CuS nanoclusters (NCs) to achieve photothermal antibacterial activity under NIR irradiation. The MUH-coated CuS NCs exhibited excellent stability in solutions with various pH values and stayed stable in pure water for at least 10 months. The MUH-coated CuS NCs showed strong antibacterial activity toward E. coli bacteria at 800 μM (76.8 μg·mL−1) concentration.

4. CuS with Protein

Despite numerous advantages of CuS nanomaterials for biomedical applications, toxicity remains an issue that needs to be resolved. To address this issue, researchers have tried to coat CuS with proteins, especially bovine serum albumin (BSA). BSA is selected as a coating for CuS due its various advantageous properties, such as versatility, non-toxicity, stability, and biodegradability. Additionally, its functional groups allow easy binding and capping of nanomaterials, such as CuS.
Zhao et al. [24] applied BSA-CuS NPs to the treatment of diabetic wound infection in vivo as a method of photothermal therapy. A facile biomineralization method was used to synthesize BSA-CuS nanomaterials, which were successfully used to destroy A. baumannii, S. aureus, and S. haemolyticus under NIR irradiation (808 nm, 8.0 W·cm−2) for 10 min at 200 mg·L−1 concentration. Both in vitro and in vivo experiments were performed in this case. The BSA-CuS NPs showed excellent NIR-mediated photothermal antibacterial activity, with low cytotoxicity, in vivo toxicity, and excellent water solubility. Dual-mode PTT and PDT was applied using Ce6-labeled BSA-CuS NPs, enabling a synergistic effect against E. coli and S. aureus both in vitro and in vivo [25]. Most importantly, the same NIR light source was utilized to generate the ROS required for PDT activity and for PTT. The CuS NPs were prepared in the presence of BSA, which prevents aggregation of the CuS NPs. BSA also acts as a linker between the CuS and Ce6 molecules through amide bonds. The Ce6-labeled BSA-CuS NPs showed an overall bacterial killing efficacy of 97% in vitro due to the synergistic effects of combined PTT and PDT. The nanocomposite also successfully eliminated bacterial infection in vivo. BSA was also utilized as a template to synthesize uniform CuS NPs by a biomineralization method, and its NIR-mediated photothermal antibacterial activity against E. coli and S. aureus was evaluated. The as-prepared BSA-CuS nanocomposites showed good biocompatibility with skin fibroblast cells. Moreover, the nanocomposites showed excellent antibacterial activity under NIR light (980 nm, 1.59 W·cm−2). The biocompatible CuS-BSA/lysozyme nanocomposite was also effective against B. subtilis and E. coli under NIR irradiation (980 nm, 0.7 W·cm−2) [26].

5. CuS with Metals

The use of intrinsic antibacterial agents, such as Ag [9][14], Au [15], and ZnO [14][27], in combination with CuS to achieve photothermal antibacterial activity, is still in the infancy stage. Addae et al. [28] demonstrated the strong photothermal antibacterial potential of Au/CuS NPs against Bacillus anthracis. The antibacterial effect was dependent on both the NP concentration and treatment time. Similarly, Park et al. [29] showed the photothermal antibacterial potential of Au@CuS against E. coli. The Au@CuS yolk-shell nanomaterials on a PDMS film induced excellent photothermal sterilization against E. coli, where the photothermal efficiency of the nanocomposite reached 79.8%. Further research in this area is still necessary to completely understand the antibacterial mechanism of the nanocomposites.

References

  1. Qiao, Y.; Ping, Y.; Zhang, H.; Zhou, B.; Liu, F.; Yu, Y.; Xie, T.; Li, W.; Zhong, D.; Zhang, Y.; et al. Laser-Activatable CuS Nanodots to Treat Multidrug-Resistant Bacteria and Release Copper Ion to Accelerate Healing of Infected Chronic Nonhealing Wounds. ACS Appl. Mater. Interfaces 2019, 11, 3809–3822.
  2. Ren, Y.; Yan, B.; Wang, P.; Yu, Y.; Cui, L.; Zhou, M.; Wang, Q. Construction of a Rapid Photothermal Antibacterial Silk Fabric via QCS-Guided In Situ Deposition of CuSNPs. ACS Sustain. Chem. Eng. 2022, 10, 2192–2203.
  3. Gargioni, C.; Borzenkov, M.; D’Alfonso, L.; Sperandeo, P.; Polissi, A.; Cucca, L.; Dacarro, G.; Grisoli, P.; Pallavicini, P.; D‘Agostino, A.; et al. Self-Assembled Monolayers of Copper Sulfide Nanoparticles on Glass as Antibacterial Coatings. Nanomaterials 2020, 10, 352.
  4. Mutalik, C.; Okoro, G.; Krisnawati, D.I.; Jazidie, A.; Rahmawati, E.Q.; Rahayu, D.; Hsu, W.T.; Kuo, T.R. Copper sulfide with morphology-dependent photodynamic and photothermal antibacterial activities. J. Colloid Interface Sci. 2022, 607, 1825–1835.
  5. Derakhshi, M.; Daemi, S.; Shahini, P.; Habibzadeh, A.; Mostafavi, E.; Ashkarran, A.A. Two-Dimensional Nanomaterials beyond Graphene for Biomedical Applications. J. Funct. Biomater. 2022, 13, 27.
  6. Naskar, A.; Kim, S.; Kim, K.-s. A nontoxic biocompatible nanocomposite comprising black phosphorus with Au-γ-Fe2O3 nanoparticles. RSC Adv. 2020, 10, 16162–16167.
  7. Naskar, A.; Lee, S.; Ko, D.; Kim, S.; Kim, K.-s. Bovine Serum Albumin-Immobilized Black Phosphorus-Based γ-Fe2O3 Nanocomposites: A Promising Biocompatible Nanoplatform. Biomedicines 2021, 9, 858.
  8. Ansari, M.O.; Gauthaman, K.; Essa, A.; Bencherif, S.A.; Memic, A. Graphene and Graphene-Based Materials in Biomedical Applications. Curr. Med. Chem. 2019, 26, 6834–6850.
  9. Naskar, A.; Khan, H.; Sarkar, R.; Kumar, S.; Halder, D.; Jana, S. Anti-biofilm activity and food packaging application of room temperature solution process based polyethylene glycol capped Ag-ZnO-graphene nanocomposite. Mater. Sci. Eng. C Mater. Biol. Appl. 2018, 91, 743–753.
  10. Huang, J.; Ho, W.; Wang, X. Metal-free disinfection effects induced by graphitic carbon nitride polymers under visible light illumination. Chem. Commun. 2014, 50, 4338–4340.
  11. Mirzaei, A.; Peymanfar, R.; Javanshir, S.; Fallahi, R.; Karimi, J. Antibacterial Characteristics of CuS Nanoplates Anchored onto g-C3N4 Nanosheets, Suspended in PMMA Matrix. Int. J. Nanosci. Nanotechnol. 2021, 17, 249–260.
  12. Zheng, K.; Li, S.; Jing, L.; Chen, P.Y.; Xie, J. Synergistic Antimicrobial Titanium Carbide (MXene) Conjugated with Gold Nanoclusters. Adv. Healthc. Mater. 2020, 9, e2001007.
  13. Li, M.; Wang, S.; Li, R.; Wang, Y.; Fan, X.; Gong, W.; Ma, Y. The Mechanical and Antibacterial Properties of Boron Nitride/Silver Nanocomposite Enhanced Polymethyl Methacrylate Resin for Application in Oral Denture Bases. Biomimetics 2022, 7, 138.
  14. Naskar, A.; Shin, J.; Kim, K.-s. A MoS2 based silver-doped ZnO nanocomposite and its antibacterial activity against β-lactamase expressing Escherichia coli. RSC Adv. 2022, 12, 7268–7275.
  15. Naskar, A.; Lee, S.; Kim, K.-s. Au-ZnO Conjugated Black Phosphorus as a Near-Infrared Light-Triggering and Recurrence-Suppressing Nanoantibiotic Platform against Staphylococcus aureus. Pharmaceutics 2021, 13, 52.
  16. Naskar, A.; Cho, H.; Kim, K.S. Black phosphorus-based CuS nanoplatform: Near-infrared-responsive and reactive oxygen species-generating agent against environmental bacterial pathogens. J. Environ. Chem. Eng. 2022, 10, 108226.
  17. Naskar, A.; Kim, K.-s. Black phosphorus nanomaterials as multi-potent and emerging platforms against bacterial infections. Microb. Pathog. 2019, 137, 103800.
  18. Cho, H.; Naskar, A.; Lee, S.; Kim, S.; Kim, K.-s. A New Surface Charge Neutralizing Nano-Adjuvant to Potentiate Polymyxins in Killing Mcr-1 Mediated Drug-Resistant Escherichia coli. Pharmaceutics 2021, 13, 250.
  19. Lv, R.; Liang, Y.-Q.; Li, Z.-Y.; Zhu, S.-L.; Cui, Z.-D.; Wu, S.-L. Flower-like CuS/graphene oxide with photothermal and enhanced photocatalytic effect for rapid bacteria-killing using visible light. Rare Met. 2022, 41, 639–649.
  20. Liu, X.; Li, X.; Shan, Y.; Yin, Y.; Liu, C.; Lin, Z.; Kumar, S.S. CuS nanoparticles anchored to g-C3N4 nanosheets for photothermal ablation of bacteria. RSC Adv. 2020, 10, 12183–12191.
  21. Li, Q.; Wang, W.; Feng, H.; Cao, L.; Wang, H.; Wang, D.; Chen, S. NIR-triggered photocatalytic and photothermal performance for sterilization based on copper sulfide nanoparticles anchored on Ti3C2Tx MXene. J. Colloid Interface Sci. 2021, 604, 810–822.
  22. Dai, X.; Zhao, Y.; Yu, Y.; Chen, X.; Wei, X.; Zhang, X.; Li, C. Single Continuous Near-Infrared Laser-Triggered Photodynamic and Photothermal Ablation of Antibiotic-Resistant Bacteria Using Effective Targeted Copper Sulfide Nanoclusters. ACS Appl. Mater. Interfaces. 2017, 9, 30470–30479.
  23. Wang, H.Y.; Hua, X.W.; Wu, F.G.; Li, B.; Liu, P.; Gu, N.; Wang, Z.; Chen, Z. Synthesis of ultrastable copper sulfide nanoclusters via trapping the reaction intermediate: Potential anticancer and antibacterial applications. ACS Appl. Mater. Interfaces 2015, 7, 7082–7092.
  24. Zhao, Y.; Cai, Q.; Qi, W.; Jia, Y.; Xiong, T.; Fan, Z.; Liu, S.; Yang, J.; Li, N.; Chang, B. BSA-CuS Nanoparticles for Photothermal Therapy of Diabetic Wound Infection In Vivo. ChemistrySelect 2018, 3, 9510–9516.
  25. Gao, D.Y.; Ji, X.; Wang, J.L.; Wang, Y.T.; Li, D.L.; Liu, Y.B.; Chang, K.W.; Qu, J.L.; Zheng, J.; Yuan, Z. Engineering a protein-based nanoplatform as an antibacterial agent for light activated dual-modal photothermal and photodynamic therapy of infection in both the NIR I and II windows. J. Mater. Chem. B 2018, 6, 732–739.
  26. Swaidan, A.; Ghayyem, S.; Barras, A.; Addad, A.; Szunerits, S.; Boukherroub, R. Enhanced Antibacterial Activity of CuS-BSA/Lysozyme under Near Infrared Light Irradiation. Nanomaterials 2021, 11, 2156.
  27. Gupta, J.; Mohapatra, J.; Bahadur, D. Visible light driven mesoporous Ag-embedded ZnO nanocomposites: Reactive oxygen species enhanced photocatalysis, bacterial inhibition and photodynamic therapy. Dalton Trans. 2017, 46, 685–696.
  28. Addae, E.; Dong, X.; McCoy, E.; Yang, C.; Chen, W.; Yang, L. Investigation of antimicrobial activity of photothermal therapeutic gold/copper sulfide core/shell nanoparticles to bacterial spores and cells. J. Biol. Eng. 2014, 8, 11.
  29. Park, E.; Selvaraj, R.; Kim, Y. High-efficiency photothermal sterilization on PDMS film with yolk-shell nanoparticles. J. Ind. Eng. Chem. 2022, 113, 522–529.
More
Information
Contributors MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to https://encyclopedia.pub/register : ,
View Times: 414
Entry Collection: Biopharmaceuticals Technology
Revisions: 2 times (View History)
Update Date: 01 Nov 2022
1000/1000