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Gravity is fundamental factor determining all processes of development and vital activity on Earth. During evolution, a complex mechanism of response to gravity alterations was formed in multicellular organisms. It includes the “gravisensors” in extracellular and intracellular spaces. Inside the cells, the cytoskeleton molecules are the principal gravity-sensitive structures, and outside the cells these are extracellular matrix (ECM) components. The cooperation between the intracellular and extracellular compartments is implemented through specialized protein structures, integrins. The gravity-sensitive complex is a kind of molecular hub that coordinates the functions of various tissues and organs in the gravitational environment. The functioning of this system is of particular importance under extremal conditions, such as spaceflight microgravity.
Object | Cell Type | Duration | Matrisome | Exp. Approach | Study | ||
---|---|---|---|---|---|---|---|
Core Matrisome | ECM-Associated Molecules | ||||||
Collagens | Proteoglycans, Glycoproteins |
||||||
Space flight | |||||||
Medaka fish | OB, Ocl (ISS) | 1, 5, 8 d | U: COL10A1 | U: OCN | U: MMP9 | DsRed live-imaging, GGA | [15] |
Mouse | MG-63, osteoinduced (Foton 10) | 9 d | D: COL1A; ND: collagen type I | D: OCN | RT-PCR, WB | [16] | |
Mouse | 2T3 OB (STS-54) | 6 d | D: OCN | RT-PCR | [17] | ||
Chicken | OB (STS-59) | 12 d | D: COL1A; ND: collagen type I | D: OCN | RT-PCR, WB | [18] | |
Rat | OB (STS-65) | 5 d | D: OCN | RT-PCR | [19] | ||
Human | OB, MSC-derived (SJ-10) | 2 d | D: COL1A1, -1A2, -3A1, -4A1, -5A1, -6A1, -8A1 | D: TNC, COMP | U: MMP1 | RT-PCR | [20] |
Human | OB, MSC-derived (SJ-10) | 5 d | D: COL1A1 | RT-PCR | [20] | ||
Hind-limb suspension (HS) | |||||||
Rat | MSC-BM, tibia | 5 d | D: OCN | RT-PCR | [17] | ||
Rat | MSC-BM, tibia | 14 d | U: OCN | RT-PCR | [17] | ||
Rat | MSC-BM, femur | 28 d | D: RUNX2, COLI, ALP, OCN | D: osteogenic potential; expression of osteoblast gene marker mRNAs under osteogenic conditions. | RT-PCR, HS | [21] | |
Mouse | MSC-BM, femur | 7 d | D: mineralization | HS | [22] | ||
Rotating-Wall Vessel (RWV) | |||||||
Human | MSC-BM | 7 d | D: COL2A1, COL10A1 | D: ON | RT-PCR | [23] | |
Mouse | OB MC3T3-E1 | 1–14 d | ND: COL1A2 | ND: OCN | RT-PCR | [23] | |
Mouse | OB/OC MLO-A5 | 1–14 d | ND: COL1A3 | ND: OCN, OPN | RT-PCR | [24] | |
Mouse | MSC C3H10T(1/2) | 1–14 d | ND: OCN, OPN, COL1A4 | ND: OCN, OPN | RT-PCR | [24] | |
Mouse | OB MC3T3-E1 | 1 d | D: OCN | RT-PCR | [25] | ||
Human | MG-63, osteoinduced | 3 d | D: COL1 | D: OCN | RT-PCR | [26] | |
Human | ChB, MSC-derived | 21 d | D: COL1 | D: AGN | RT-PCR | [27] | |
Mouse | OB | 1d | ND: COL1A2 | ND: OCN, OPN | RT-PCR | [28] | |
Random Positioning Machine (RPM) | |||||||
Human | FB | 3 d | U: COL4A5 | U: FN/FN | U: TGF | RT-PCR, WB | [3] |
Human | MSC-AT | 4 d | U: COL12A1, COL15A1, COL16A1, COL1A1, COL5A1, COL8A1 | U: THBS1, THBS2, THBS3, LAMA, SPARC, TNC, VCAN, VTN; D: CLEC3B | RT-PCR | [29] | |
Human | MSC-AT | 10 d | D: COL11A1; D: collagenous proteins | D: LAMB3, TNC; U: non-collagenous proteins | RT-PCR, HC | [30] | |
Human | MSC-BM, osteoinduced | 20 d | U: COL1A1 | D: OMD; ND: OCN | D: ECM mineralization | RT-PCR, HC | [31] |
Human | MSC-BM | 5 d | D: COL9A1, COL2A1 | RT-PCR | [32] | ||
Human | MSC-BM, osteoinduced | 10 d | ND: COL1A1 | D: OMD; ND: OCN | RT-PCR, ICC | 77] | |
Human | MSC-BM | 20 d | ND: COL1A1; ND: collagen tot | RT-PCR, ICC | [32] | ||
Human | OB | 20 d | U: ECM mineralization | HC | [32] | ||
Mouse | 2T3 OB | 3 d | D: OMD | RT-PCR | [33] | ||
2D, 3D-clinorotation | |||||||
Mouse | MC3T3-E2 | 3 d | U: PLOD1, PLOD2; U: enzymes activity | RT-PCR, enzyme assay | [34] | ||
Human | MSC-AT | 7 d | U: COL1 COL3 | D: FBN1 | D: MMP1 | RT-PCR | [35] |
Mouse | MC3T3-E1 | 7 d | D: COL1A1 | RT-PCR | [36] | ||
Rat | MSC | 1–4 d | D: cbfa1/RUNX2 | RT-PCR | [37] |
Protein | Uniprot Index | Biological Process (ANDCell) |
---|---|---|
Alpha-2-HS-glycoprotein | FETUA_HUMAN | Positive regulation of ECM constituent secretion; Regulation of ECM assembly; erk 1/2 mitogen-activated protein kinase pathway ANG 2; ANG2 expression of ECM proteins; ANG2 erk1/2 pathway; mek/erk pathway; erk pathway |
Angiotensinogen | ANGT_HUMAN | erk pathway |
Apolipoprotein A-I | APOA1_HUMAN | erk pathway |
Apolipoprotein E | APOE_HUMAN | Positive regulation of ECM constituent secretion |
Carboxypeptidase B2 | CBPB2_HUMAN | erk pathway |
Cathelicidin antimicrobial peptide | CAMP_HUMAN | ras-erk pathway |
CD44 antigen | CD44_HUMAN | MMP9 signaling pathway; ras-erk1/2 pathway; mek/erk pathway; erk pathway. |
Clusterin | CLUS_HUMAN | Inhibition of ECM disassembly; ECM organization; mapk/erk pathway; MMP9 signaling pathway |
Cystatin-C | CYTC_HUMAN | ECM organization |
Fibronectin | FINC_HUMAN | ECM organization; Activation of erk pathway; ECM assembly; erk1/2 pathway; Cell–matrix adhesion; erk pathway; Calcium independent cell matrix adhesion; mapk/erk pathway |
Fibulin-1 | FBLN1_HUMAN | Cell–matrix adhesion |
Insulin-like growth factor-binding protein 3 | IBP3_HUMAN | erk1/2 pathway |
Intercellular adhesion molecule 1 | ICAM1_HUMAN | erk1/2 pathway; mek/erk pathway; Cell–matrix adhesion |
Kininogen-1 | KNG1_HUMAN | Bradykinin in MMP secretion; ECM secretion; mapk/erk pathway |
Lumican | LUM_HUMAN | ECM assembly |
Pigment epithelium-derived factor | PEDF_HUMAN | MMP secretion; apoptotic signaling pathway; erk1/2 pathway |
Transthyretin | TTHY_HUMAN | Apoptotic signaling pathway; erk1/2 pathway |
Vitronectin | VTNC_HUMAN | ECM organization; Cell–matrix adhesion |