- Please check and comment entries here.
DNA-Dependent Protein Kinase Catalytic Subunit
The DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is a key component of the DNA-PK complex that has a well-characterized function in the non-homologous end-joining repair of DNA double-strand breaks. Since its identification, a large body of evidence has demonstrated that DNA-PKcs is frequently overexpressed in cancer, plays a critical role in tumor development and progression, and is associated with poor prognosis of cancer patients.
The DNA-dependent protein kinase (DNA-PK) is a large holoenzyme composed of the DNA-PK catalytic subunit (DNA-PKcs) and a heterodimer of Ku proteins (Ku70/Ku80) . It was originally identified as part of the SP1 (specificity protein 1) transcription complex and as a regulatory component of transcriptionally poised RNA polymerase II (RNAPII) . Subsequently, it has been studied in detail concerning its function in DNA double-strand break (DSB) repair, especially in the non-homologous end joining (NHEJ) repair pathway .
Consistent with the role in maintaining genomic instability, a hallmark of cancer, a considerable number of studies have demonstrated the association between dysregulated DNA-PKcs and cancer development . Overexpression of DNA-PKcs is frequent in a variety of cancer types and predicts poor prognosis in patients . Consequently, DNA-PKcs has been shown to be a potential therapeutic target, especially in combination with radio- and chemotherapy that induce DNA damage .
Intriguingly, recent studies have revealed novel functions of DNA-PKcs in cancer, such as cell cycle progression, tumor metastasis and resistance to therapy, metabolic regulation, and tumor immunity , which transforms the paradigm of DNA-PKcs and expands the utility of DNA-PKcs inhibitors in cancer treatment. Nevertheless, the molecular underpinnings for the pleiotropy of DNA-PKcs remain largely unknown.
In the light of an increasing spectrum of DNA-PKcs functions, therapeutic targeting of DNA-PKcs has regained the momentum as a promising cancer therapy. Reflecting this, numerous highly selective DNA-PKcs inhibitors such as NU7441, M3814, AZD7648, and NU5455 have been developed  and are being evaluated in clinical trials (NCT03770689, NCT04172532, NCT04533750). Although some promising preclinical and early-phase clinical results have been reported recently , the precise management of DNA-PKcs-based therapy for cancer patients remains a challenge due to the physiological role of DNA-PKcs and the lack of biomarkers that predict response to DNA-PKcs inhibitors. Therefore, the identification of biomarkers for DNA-PKcs inhibitor sensitivity is of great importance, not only for the development of precision therapeutic options based on DNA-PKcs inhibitor drugs but also for the mechanistic understanding of DNA-PKcs regulation. The classical role of DNA-PKcs in DNA damage response has been extensively reviewed elsewhere .
2. DNA-PKcs and Cancer
DNA-PKcs overexpression is associated with poor prognosis in several cancer cohorts . In the public The Cancer Genome Atlas (TCGA) database, genetic alterations of PRKDC (Protein Kinase, DNA-Activated, Catalytic Subunit; encoding DNA-PKcs), e.g., point mutations and copy number amplifications, are common in a variety of cancer types, particularly in uterine corpus endometrial carcinoma (UCEC), uterine carcinosarcoma (UCS), skin cutaneous melanoma (SKCM), stomach adenocarcinoma (STAD), liver hepatocellular carcinoma (LIHC), lung adenocarcinoma (LUAD), and colorectal adenocarcinoma (COADRE) (frequency > 10%) (Figure 1A). High expression of PRKDC occurs in the majority of human cancers, compared to the matched normal tissue (Figure 1B), which predicts dismal patient survival in the majority of TCGA cancer types (Figure 1C). However, in hTERT (human telomerase reverse transcriptase)/CDK4 (cyclin-dependent kinase 4) immortalized human bronchial epithelial cells (HBECs), DNA-PKcs knockdown to levels simulating haploinsufficiency dramatically reduced DNA repair capacity with significantly increased transformation efficiency of HBEC lines exposed to bleomycin . The transformation enabled by compromised DNA-PKcs is mainly due to the epigenome reprogramming . Thus, DNA-PKcs may have a dual role, e.g., as a tumor suppressor in premalignant stages but an oncogenic driver in the advanced stage. Supporting this notion, the expression of PRKDC is significantly associated with histological grades (Figure 2A).
Figure 1. DNA-dependent protein kinase catalytic subunit (DNA-PKcs) in human cancer. (A) Genetic landscape of PRKDC (encoding DNA-PKcs) across the pan-cancer cohort in The Cancer Genome Atlas (TCGA). Data were downloaded from the cBioPortal database (https://www.cbioportal.org/). PRKDC was queried in TCGA pan-cancer cohort. (B) Bar plots showing the PRKDC gene expression profile across all tumor samples and paired normal tissues. The height of the bar represents the median expression of the indicated tumor (in red) or normal (in blue) tissue. (C) Forest blots showing the survival analysis of cancer patients stratified by the gene expression of PRKDC across the TCGA pan-solid cancer cohort. Only significant (p < 0.05) results were presented. The "high" and "low" expression groups were stratified by the optimal cutoff value using "survminer" and "survival" packages in R software. N, the total number in each group. Scale line indicates the 95% confidence interval for effect estimate for each survival-influencing factor with the hazard ratio showing to the right. ACC, adrenocortical carcinoma; BLCA, bladder urothelial carcinoma; BRCA, breast invasive carcinoma; CESC, cervical squamous cell carcinoma and endocervical adenocarcinoma; CHOL, cholangiocarcinoma; COAD, colon adenocarcinoma; ESCA, esophageal carcinoma; GBM, glioblastoma multiforme; HNSC, head and neck squamous cell carcinoma; KICH, kidney chromophobe; KIRC, kidney renal clear cell carcinoma; KIRP, kidney renal papillary cell carcinoma; LGG, brain lower grade glioma; LIHC, liver hepatocellular carcinoma; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; MESO, mesothelioma; OV, ovarian serous cystadenocarcinoma; PAAD, pancreatic adenocarcinoma; PCPG, pheochromocytoma and paraganglioma; PRAD, prostate adenocarcinoma; READ, rectum adenocarcinoma; SARC, sarcoma; SKCM, skin cutaneous melanoma; STAD, stomach adenocarcinoma; TGCT, testicular germ cell tumors; THCA, thyroid carcinoma; UCS, uterine carcinosarcoma; UCEC, uterine corpus endometrial carcinoma; UVM, uveal melanoma. * p < 0.05, ** p < 0.01, *** p < 0.001.
3. DNA-PKcs in Tumor Metastasis and Therapy Resistance
Metastasis and resistance to cancer therapeutics are the main cause of poor clinical outcomes. Intriguingly, there is emerging evidence that DNA-PKcs plays an important role in tumor metastasis and therapy resistance through transcriptional regulation .
By analyzing the secretome of isogenic cells differing in DNA-PKcs expression, a previous study  showed that, compared to DNA-PKcs-deficient cells, DNA-PKcs-proficient cells secrete higher levels of at least 103 proteins, including 44 metastasis-associated FBLN1 (Fibulin 1), SERPINA3 (Serpin Family A Member 3), MMP-8 (Matrix Metallopeptidase 8), and HSPG2 (Heparan Sulfate Proteoglycan 2), as well as the inhibitors of matrix metalloproteinases, such as α-2M (Alpha-2-macroglobulin) and TIMP-2 (TIMP Metallopeptidase Inhibitor 2) that are associated with a pro-metastatic activity. Consistently, clinical evidence shows that DNA-PKcs expression is dramatically elevated in tumor samples with distant metastasis . These results suggest that DNA-PKcs may facilitate metastasis by modifying the tumor microenvironment.
The importance of DNA-PKcs for metastasis has been comprehensively demonstrated in prostate cancer, in which DNA-PKcs-mediated gene regulation promotes tumor migration, invasion, and metastases while DNA-PKcs suppression inhibits metastases . In detail, gene set enrichment analysis (GSEA) of the differentially downregulated genes in prostate cancer cells with DNA-PKcs knockdown revealed significant enrichment of genes regulated by MAZ (MYC Associated Zinc Finger Protein), MYC (Myelocytomatosis), and SP1, a well-characterized DNA-PKcs-interacting partner . Of note, DNA-PKcs is highly activated in advanced tumors, which is independent of DNA damage indicators . This study convincingly showed that DNA-PKcs is a potent driver of tumor progression and metastases. Moreover, in an attempt to reveal the underlying mechanisms that mediate the aggressive phenotypes of DNA-PKcs, the same group performed unbiased investigations in multiple tumor models, which revealed that DNA-PKcs is pleiotropic, involved in the regulation of various biological processes beyond DNA repair, such as epithelial–mesenchymal transition (EMT), immune response, and metabolic processes . Supporting the findings, a recent study by Kothari et al. showed that, among other kinases, DNA-PKcs is most significantly associated with metastatic progression in high-risk prostate cancer and its inhibition suppressed the growth of both androgen receptor (AR)-dependent and AR-independent prostate cancer cells . Mechanistically, DNA-PKcs interacts with the Wnt transcription factor LEF1 (Lymphoid Enhancer Binding Factor 1) and is critical for LEF1-mediated transcription .
By using the previously curated EMT signature , we found that the gene expression of DNA-PKcs is positively correlated with the EMT signature across a pan-cancer cohort in TCGA (Figure 2B). Interestingly, we also identified a positive correlation between DNA-PKcs and a curated stemness signature , defining the status of cancer stemness that plays a crucial role in tumor initiation, relapse, and metastasis in the majority of cancer types (Figure 2B).
In addition to promoting tumor metastasis, preclinical evidence also revealed that DNA-PKcs is associated with primary resistance to chemo- and targeted therapy . However, regarding its role in mediating resistance to therapy, the extent to which these functions of DNA-PKcs are independent of DNA repair has not been well clarified in these studies. Interestingly, a recent study showed that DNA repair-independent functions of DNA-PKcs protect irradiated cells from mitotic slippage and accelerated senescence .
Figure 2. The role of DNA-PKcs in cancer progression. (A) Violin plots showing the association between PRKDC expression and histological grades of TCGA tumors. Note that the information on the histological grades is only available for several cancer types. (B) Correlation analysis of the epithelial-to-mesenchymal transition (EMT; left) and stemness (right) signatures and gene expression of PRKDC across the TCGA pan-solid cancer cohort. Curated EMT  and mRNA-based stemness scores  derived by the stemness group was used. ACC, adrenocortical carcinoma; BLCA, bladder urothelial carcinoma; BRCA, breast invasive carcinoma; CESC, cervical squamous cell carcinoma and endocervical adenocarcinoma; CHOL, cholangiocarcinoma; COAD, colon adenocarcinoma; ESCA, esophageal carcinoma; GBM, glioblastoma multiforme; HNSC, head and neck squamous cell carcinoma; KICH, kidney chromophobe; KIRC, kidney renal clear cell carcinoma; KIRP, kidney renal papillary cell carcinoma; LGG, brain lower grade glioma; LIHC, liver hepatocellular carcinoma; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; MESO, mesothelioma; OV, ovarian serous cystadenocarcinoma; PAAD, pancreatic adenocarcinoma; PCPG, pheochromocytoma and paraganglioma; PRAD, prostate adenocarcinoma; READ, rectum adenocarcinoma; SARC, sarcoma; SKCM, skin cutaneous melanoma; STAD, stomach adenocarcinoma; TGCT, testicular germ cell tumors; THCA, thyroid carcinoma; UCS, uterine carcinosarcoma; UCEC, uterine corpus endometrial carcinoma; UVM, uveal melanoma.
4. DNA-PKcs in Metabolism
Metabolic dysregulation is a hallmark of cancer . Emerging evidence has associated DNA-PKcs with metabolic regulation in physiological and pathological contexts . First, DNA-PKcs can transcriptionally upregulate the genes involved in lipogenesis in response to feeding and insulin signaling . Secondly, DNA-PKcs can facilitate the activation of AMP (adenosine monophosphate)-activated protein kinase (AMPK), a central energy sensor, and, consequently, promote the restoration of energy balance in response to glucose deprivation . Moreover, blocking DNA-PKcs can rescue the age-related metabolic decline . Finally, more recent studies have also revealed that DNA-PKcs regulates cancer metabolism . In detail, the c-Myc (cellular Myelocytomatosis) oncoprotein is a master regulator controlling cellular growth and metabolism  and DNA-PKcs leads to the activation of the AKT (AKR thymoma)/GSK3 (Glycogen synthase kinase 3) pathway, thereby indirectly stabilizing c-Myc. Indeed, the silencing of PRKDC expression decreases Myc protein levels in HeLa cells . Thus, it has been suggested that DNA-PKcs has a dual role. On one side, sufficient DNA-PKcs activity is necessary to maintain genomic integrity. On the other side, dysregulated overexpression of DNA-PKcs may contribute to stabilizing the c-Myc oncoprotein, thereby contributing to the aberrant cellular metabolism characteristic for cancer cells, resulting in oncogenic transformation and aberrant cellular proliferation at the early and late stage of cancer development, respectively.
Autophagy is crucial to maintain cellular metabolic homeostasis and plays a key role in promoting the survival of cancer cells in the case of metabolic stress and facilitating the development of therapeutic resistance . DNA-PKcs-mediated NHEJ functions as the major repair pathway in response to ionizing radiation (IR), providing the rationale for combined treatment with IR and DNA-PKcs inhibitors in various cancers . However, it remains unclear as to whether the combinatorial effects are exclusively mediated by impaired DSB repair. Accumulating evidence has shown that autophagic cell death also plays a critical role in mediating the effects of IR and DNA-PKcs inhibitor combination . Taking advantage of isogenic cancer cell models (human malignant glioma), including M059J cells that lack the DNA-PKcs and the counterpart M059K cells proficient in DNA-PKcs, Daido et al.  showed that low-dose IR induced massive autophagic cell death in M059J but not in M059K cells, providing new insights into the molecular mechanisms of combined IR and DNA-PKcs inhibitor treatment, although it is still not clear whether the effect is tissue-specific.
Intriguingly, combined treatment with the DNA-PKcs inhibitor NU7441 and IR showed different effects on non-small cell lung cancer cells (A549, H460, and H1299). Specifically, NU7441 induced G2/M checkpoint and mitotic catastrophe exclusively in H1299, whereas it upregulated autophagy in A549 and induced senescence in H460, suggesting the heterogeneity of DNA-PKcs inhibitor on the choice of cell death. Of note, all the three cell lines harbor KRAS (Kirsten Rat Sarcoma Viral Oncogene Homolog) mutations, but differ in amino acid substitutions, which can engage different downstream effectors , with the genes co-mutated with KRAS also potentially playing a role . Further studies are warranted to identify factors affecting the heterogeneous effects of DNA-PKcs targeted therapy.
Questions remain with regards to how DNA-PKcs interacts with autophagy. A recent study by Puustinen et al. showed that DNA-PKcs acts upstream of AMPK and ULK1 (Unc-51 Like Autophagy Activating Kinase 1) kinase, core regulators of autophagy . Mechanistically, DNA-PKcs interacts with the AMPK complex and phosphorylated its nucleotide-sensing γ1 subunit PRKAG1 /AMPKγ1 (Protein Kinase AMP-Activated Non-Catalytic Subunit Gamma 1) at Ser192 and Thr284. DNA-PKcs-mediated phosphorylation of PRKAG1 inhibited lysosomal localization of the AMPK complex and the starvation-induced association with STK11 (serine/threonine kinase 11). These data suggest that DNA-PKcs-mediated phosphorylation of PRKAG1 primes the AMPK complex to the lysosomal activation by STK11 in cancer cells, thereby linking the function of DNA-PKcs with autophagy and cellular metabolism .
5. DNA-PKcs in Immunity
NHEJ is required for both V(D)J recombination and class switch recombination (CSR), two Ig (immunoglobulin) gene-diversification processes occurring during B cell development , and, consequently, DNA-PKcs inactivation leads to immune deficiency . The association of DNA-PKcs with B cell-mediated immunity raises the question of how DNA-PKcs dysregulation is involved in shaping the cancer immune microenvironment.
As a key component of DSB repair and recombination, DNA-PKcs plays a pivotal role in genomic stability. Microsatellite instability (MSI) status has been identified as a biomarker to predict patients who could benefit from immunotherapy. A recent study  showed that PRKDC mutation was significantly associated with a high mutation load or high-MSI status in cancer on the basis of the TCGA pan-cancer cohort and, more importantly, PRKDC knockout and DNA-PKcs inhibitor enhanced the efficacy of immunological checkpoint inhibitors (ICI). These findings highlight PRKDC mutation as a potential biomarker for immunotherapy. Moreover, Chen et al. recently investigated the association between PRKDC mutations and tumor mutation burden (TMB), tumor microenvironment (TME), and response to ICI by integrated analysis of sequencing data of solid tumors in TCGA (n = 4023) and Geneplus (n = 3877) . They showed that PRKDC mutant tumors have significantly higher TMB than that of PRKDC wild-type tumors. Further, solid tumors harboring PRKDC mutations were enriched in tumor immunogenicity microenvironments, such as CD8+ T cells, NK (natural killer) cells, and chemokines. Importantly, PRKDC mutations were associated with better survival in patients after ICI treatment . These results support the use of PRKDC mutations as a stratification marker for immunotherapy.
Nevertheless, how the expression level of DNA-PKcs affects the tumor immune microenvironment is still unknown. Our analysis of a pan-cancer cohort in the TCGA showed that solid tumors expressing high levels of PRKDC differ in the immune subtype from those with low PRKDC expression (Figure 3A), on the basis of previously curated immune subtype models . Specifically, high PRKDC tumors were enriched with IFN (interferon)-γ-dominant and wound healing subtypes, and PRKDC expression significantly correlates CD8+ T cell and B cell signatures across different cancer types (Figure 3B) . Despite the potential roles of DNA-PKcs in cancer immunity, it remains unclear as to whether targeting DNA-PKcs could affect the efficacy of ICI treatment. To improve the immunotherapy for melanoma patients, Tsai and colleagues used the unbiased high-throughput flow cytometry-based screening to identify and characterize candidate therapies that might synergize with and augment T-cell immunotherapy efficacy . Surprisingly, NU7441, a selective inhibitor of DNA-PKcs , was identified as one of the two lead therapies, as NU7441 could alter a variety of immunomodulatory proteins, e.g., CD55, CD73, CD155, programmed death-ligand 1 (PD-L1), nerve growth factor receptor (NGFR), and HLA (human leukocyte antigen) class I in a heterogeneous panel of melanomas, leading to proliferative inhibition of melanoma cells . Consequently, targeting DNA-PKcs enhances the efficacy of immunotherapies. These findings provided mechanistic insights and a strong rationale for co-targeting DNA-PKcs to improve immunotherapy.
Figure 3. PRKDC expression is associated with immune cell infiltrates across diverse cancer types. (A) Percentage (PRKDC expression low vs. high) of immune subtype models (C1–C6) across TCGA pan-cancer cohort. The genes contained in each signature were evaluated using model-based clustering by p the "mclust" R package. Each sample was finally to be grouped on the basis of its predominance with the C1-C6 signature. The immune subtype models were based on Thorsson et al. (2018) . (B) Systematic correlation analysis of immune infiltrates (CD8+, left; CD4+, right) with gene expression of PRKDC across TCGA pan-cancer cohort. The number of patients was shown in parenthesis. Data were downloaded from TIMER (version 2.0; with "PRKDC" as a query), a comprehensive resource for systematic analysis of immune infiltrates across diverse cancer types (http://timer.comp-genomics.org/) . The red color indicates a positive correlation (0–1), while the blue color represents a negative correlation (−1–0). The correlation with p-value < 0.05 is considered as significant. The correlation coefficient values with p-value > 0.05 are marked with a cross. ACC, adrenocortical carcinoma; BLCA, bladder urothelial carcinoma; BRCA, breast invasive carcinoma; CESC, cervical squamous cell carcinoma and endocervical adenocarcinoma; CHOL, cholangiocarcinoma; COAD, colon adenocarcinoma; ESCA, esophageal carcinoma; GBM, glioblastoma multiforme; HNSC, head and neck squamous cell carcinoma; KICH, kidney chromophobe; KIRC, kidney renal clear cell carcinoma; KIRP, kidney renal papillary cell carcinoma; LGG, brain lower grade glioma; LIHC, liver hepatocellular carcinoma; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; MESO, mesothelioma; OV, ovarian serous cystadenocarcinoma; PAAD, pancreatic adenocarcinoma; PCPG, pheochromocytoma and paraganglioma; PRAD, prostate adenocarcinoma; READ, rectum adenocarcinoma; SARC, sarcoma; SKCM, skin cutaneous melanoma; STAD, stomach adenocarcinoma; TGCT, testicular germ cell tumors; THCA, thyroid carcinoma; UCS, uterine carcinosarcoma; UCEC, uterine corpus endometrial carcinoma; UVM, uveal melanoma.
6. DNA-PKcs Interactors
Although the pleiotropic role of DNA-PKcs—DNA repair-dependent or -independent—have been well established, the way in which the activity of DNA-PKcs is mediated is still not fully elucidated. DNA-PKcs and the regulatory subunits Ku70/80 form with a 1:1:1 stoichiometry the DNA-PK complex, which has serine/threonine protein kinase activity and plays a pivotal role in the repair and recovery from DNA damage . In addition, DNA-PKcs phosphorylates a variety of transcription factors and the large subunit of RNA polymerase II, thereby indirectly regulating gene transcription . Additionally, DNA-PKcs is a large protein with multiple domains that can potentially interact with plenty of other proteins within a cell , which might contribute to the multifaceted roles of DNA-PKcs .
A recent study by Song and colleagues systematically explored the DNA-PKcs–RNA interactome and characterized the genome-wide landscape of DNA-PKcs-associated RNAs on the basis of RNA immunoprecipitation coupled with next-generation sequencing (RIP-seq) . Approximately 500 RNAs were co-precipitated with DNA-PKcs with a stringent cutoff. Subsequent pathway enrichment analysis showed that the RNAs bound to DNA-PKcs were mainly involved in the focal adhesion and receptor–ECM (extracellular matrix) interaction signaling pathways, indicating that DNA-PKcs may control a variety of biological processes partially through its RNA-binding activity.
Through integrated analysis of the publicly curated protein interaction databases (Protein—interacting data downloaded from Agile Protein Interactomes DataServer (APID, http://cicblade.dep.usal.es:8080/APID/init.action); BioGRID, version 4.0; https://thebiogrid.org/; HitPredict, http://www.hitpredict.org/), we identified 136 common interacting proteins with DNA-PKcs (Figure 4A). Pathway analysis showed that these proteins are enriched in diverse cellular processes apart from DNA repair (Figure 4B), e.g., regulation of processes related to DNA metabolism, response to oxidative stress, and telomere maintenance, which were also observed in previous studies . Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that these proteins were significantly enriched in multiple cancer types (Figure 4C).
Figure 4. Pathway analysis of DNA-PKcs interacting proteins. (A) Venn diagram showing the proteins commonly interacting with DNA-PKcs on the basis of three publicly curated datasets (Agile Protein Interactomes DataServer (http://cicblade.dep.usal.es:8080/APID/init.action), BioGRID (version 4.0; https://thebiogrid.org/), and HitPredict (http://www.hitpredict.org/)) using PRKDC as input. Venn diagram was generated using "VennDiagram" package in R software. (B,C) Top 10 significantly enriched GO (Gene Ontology; biological process, BP) and Kyoto Encyclopedia of Genes and Genomes (KEGG) (C) pathways based on proteins commonly interacting with DNA-PKcs. Cnetplot in B (right) listed gene names of the proteins enriched in the GO pathway. The KEGG and GO pathway enrichment analyses were performed using the R package "clusterprofiler".
Overall, the evidence above suggests that DNA-PKcs can regulate a variety of pathways while its own activity is modulated by a network of interacting pathways. Thus, DNA-PKcs serves as a central node of multiple interconnected signaling pathways to orchestrate diverse cellular processes including DNA repair, metabolism, and transcription.
7. Biomarkers for Sensitivity to DNA-PKcs Targeted Therapy
DNA-PKcs inhibitors have been shown to be essential in several cancers and these inhibitors alone have been shown to strongly affect the survival of cancer cells of different lineages  despite ambivalent responses , highlighting the need for biomarkers to improve the clinical utility of DNA-PKcs-targeted therapy.
TP53 (Tumor Protein P53) mutational status was identified as a biomarker for therapeutic response to combined treatment with radiation and DNA-PKcs targeted therapy . Specifically, cancer cells with dysfunctional p53 were unable to fully arrest their cell cycle and thus entered S and M phases with unrepaired DNA, leading to mitotic catastrophe and apoptotic cell death. In contrast, in cancer cells with wild-type TP53; ataxia telangiectasia-mutated (ATM); and its targets, p53 and checkpoint kinase 2 (CHK2), were more strongly activated by the addition of DNA-PKcs inhibitor M3814, leading to a complete p53-dependent cell-cycle block and premature cell senescence . Supporting this notion, pharmacological or genetic abrogation of DNA-PKcs leads to apoptotic death of ATM-defective cells, highlighting DNA-PKcs inhibitors being a promising treatment for ATM-defective malignancies. By integrating the mutational landscape of 1319 cancer-associated genes with sensitivity data of DNA-PKcs inhibition across 67 cell lines , Dietlein and colleagues identified a considerable number of genes involved in homologous recombination-mediated DNA repair, including BRCA1 (Breast And Ovarian Cancer Susceptibility Protein 1), BRCA2 (Breast And Ovarian Cancer Susceptibility Protein 2), ATM (Ataxia Telangiectasia Mutated), PAXIP1 (paired box paired box 1), and RAD50 (DNA repair protein RAD50), whose mutations led to non-oncogene addiction to DNA-PKcs. In particular, mutations in the mismatch repair gene MSH3 are the most significant predictors of DNA-PKcs addiction. Consequently, DNA-PKcs inhibition robustly induced apoptosis in MSH3-mutant tumors in vitro and in vivo. Thus, the mutational status in TP53, ATM, and MSH3 should be taken into account for the future design of clinical trials.
Synergistic combination therapy with DNA-PKcs inhibitors has also been investigated, especially with chemo- and radiotherapy . More recently, combined PARP (Poly (ADP-ribose) polymerase)-targeted therapy and DNA-PKcs inhibitor have been demonstrated to synergistically inhibit tumor growth in both orthotopic mouse and patient-derived xenograft models of hepatocellular carcinoma . Importantly, the combination regimen showed low toxicity, as no significant influence on liver, kidney, heart, and body weight was observed .
It has been shown that DNA-PKcs is important for sustained activation of the AKT signaling pathway , which is interlinked with EMT, therapy resistance, and cancer metabolism . These observations suggest that activation of the AKT signaling pathway in tumors may serve as a potential biomarker for response to DNA-PKcs-targeted therapy.
We mined drug sensitivity data of NU7441, a selective DNA-PKcs inhibitor , in GDSC (Genomics of Drug Sensitivity in Cancer, https://www.cancerrxgene.org/) and showed that cancer cell lines from soft tissue and thyroid are particularly sensitive to NU7441, whereas cancer cell lines with other lineages display highly heterogeneous responses to NU7441 (Figure 5A,B). To systematically probe the biomarkers for response to DNA-PKcs inhibitors, we correlated the drug response profiles of NU7441 and the protein reverse phase (RPPA) dataset of TCPA (The Cancer Proteome Atlas) cancer cell line cohort (https://tcpaportal.org/), which identified several proteins whose expression is significantly correlated, negatively and positively, with the IC50 value of NU7441 (Figure 5C). It is noteworthy that a negative correlation indicates that a higher protein content correlates with a lower IC50 value and thus represents a biomarker of sensitivity to treatment and vice versa. S6, a key downstream effector of the AKT/mTOR (mechanistic target of rapamycin) signaling pathway, is most negatively correlated with NU7441 (high S6 level and low IC50), which is in line with the role of DNA-PKcs in activating AKT (Figure 5C) . Of particular interest, E-cadherin and claudin 7 (typical epithelial markers) are positively correlated with the IC50 value (Figure 5C), indicating that DNA-PKcs inhibitors preferentially target mesenchymal cells that are actively involved in tumor metastasis and therapy resistance .
Figure 5. Biomarkers predicting therapeutic response to the DNA-PKcs inhibitor NU7441. (A) The molecular structure of NU7441 compound. (B) IC50 (half maximal inhibitory concentration) values of cancer cell lines with different lineages to NU7441. Data were downloaded and reanalyzed from GDSC (Genomics of Drug Sensitivity in Cancer, https://www.cancerrxgene.org/). (C) Integrated correlation analysis of IC50 of NU7441 and protein array data across cancer cell lines (left) and solid cancer cell lines (right). Red dots indicate proteins whose expression level significantly (p < 0.05) positively correlates with the effectiveness (IC50 value) of NU7441, while the blue dots represent the significantly negatively correlated proteins. The protein array data were downloaded from TCPA (The Cancer Proteome Atlas) cancer cell line cohort (https://tcpaportal.org/), which contains 216 proteins critical for cancer.
Of note, although numerous clinical trials (NCT03724890, NCT03770689, NCT03983824, NCT04071236, NCT04172532, NCT02316197, NCT04092270) with DNA-PKcs inhibitors are ongoing, few involve biomarker-guided patient stratification, which is critical, as we discuss above.
8. Toxicity Concern of DNA-PKcs Targeted Therapy
DNA-PKcs plays essential functions in both normal tissues and tumors , raising the possibility of toxicity concern of DNA-PKcs-targeted therapy in vivo. Mice with PRKDC knockout exhibit severe combined immune-deficiency and increased sensitivity to ionizing radiation . Further, compared to wild-type mice, DNA-PKcs-null mice have reduced viability and display an earlier onset of aging-related pathologies, e.g., thymus lymphoma, infection, and intestinal atrophy, as well as compromised telomere activity due to the role of DNA-PKcs in the maintenance of the telomere length . Notably, the toxicity of DNA-PKcs inhibitors is expected to be significantly increased when administered in combination with DNA-damaging agents, given that they directly block the DNA repair machinery. Note that DNA-PKcs deletion is not identical as pharmacological inhibition , with the latter typically inducing a transient inhibition of its kinase activity that may explain the reported tolerability of selective DNA-PKcs inhibitors , although long-term toxicity data are still missing.
The potential side effects may be overridden by co-targeting other genes, e.g., the aforementioned MSH3, ATM, BRCA1, and BRCA2, whose inactivation is synthetic lethal with DNA-PKcs inhibitors.
The tumor hypoxic microenvironment plays a critical role in malignant phenotypes and therapy resistance. The importance of DNA-PKcs for tumors cells in response to hypoxic microenvironment has been well documented , which can also be taken into account to enhance the efficacy of DNA-PKcs inhibitors , thereby preferentially affecting the tumor cells and reducing the adverse effects. Intriguingly, Wong et al. recently showed that SN38023, a novel prodrug, can be metabolized to a potent DNA-PKcs inhibitor (IC87361) selectively in hypoxic cells , such that SN38023 selectively inhibited radiation-activated Ser2056 autophosphorylation of DNA-PKcs and radio-sensitized cancer cells under anoxia , indicating the promise to exploit hypoxia for selective delivery of DNA-PKcs inhibitors to target tumor cells.
This entry is adapted from 10.3390/cancers12113389
- Dvir, A.; Peterson, S.R.; Knuth, M.W.; Lu, H.; Dynan, W.S. Ku autoantigen is the regulatory component of a template-associated protein kinase that phosphorylates RNA polymerase II. Proc. Natl. Acad. Sci. USA 1992, 89, 11920–11924.
- Gottlieb, T.M.; Jackson, S.P. The DNA-dependent protein kinase: Requirement for DNA ends and association with Ku antigen. Cell 1993, 72, 131–142, doi:10.1016/0092-867490057-w.
- Falck, J.; Coates, J.; Jackson, S.P. Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nat. Cell Biol. 2005, 434, 605–611, doi:10.1038/nature03442.
- Blackford, A.N.; Jackson, S.P. ATM, ATR, and DNA-PK: The Trinity at the Heart of the DNA Damage Response. Mol. Cell 2017, 66, 801–817, doi:10.1016/j.molcel.2017.05.015.
- Medová, M.; Medo, M.; Hovhannisyan, L.; Muñoz-Maldonado, C.; Aebersold, D.M.; Zimmer, Y. DNA-PK in human malignant disorders: Mechanisms and implications for pharmacological interventions. Pharmacol. Ther. 2020, 215, 107617, doi:10.1016/j.pharmthera.2020.107617.
- Toulany, M.; Lee, K.-J.; Fattah, K.R.; Lin, Y.-F.; Fehrenbacher, B.; Schaller, M.; Chen, B.P.; Chen, D.J.; Rodemann, H.P. Akt Promotes Post-Irradiation Survival of Human Tumor Cells through Initiation, Progression, and Termination of DNA-PKcs-Dependent DNA Double-Strand Break Repair. Mol. Cancer Res. 2012, 10, 945–957, doi:10.1158/1541-7786.mcr-11-0592.
- Goodwin, J.F.; Kothari, V.; Drake, J.M.; Zhao, S.; Dylgjeri, E.; Dean, J.L.; Schiewer, M.J.; McNair, C.; Jones, J.K.; Aytes, A.; et al. DNA-PKcs-Mediated Transcriptional Regulation Drives Prostate Cancer Progression and Metastasis. Cancer Cell 2015, 28, 97–113, doi:10.1016/j.ccell.2015.06.004.
- Teneng, I.; Picchi, M.A.; Leng, S.; Dagucon, C.P.; Ramalingam, S.; Tellez, C.S.; Belinsky, S.A. DNA-PKc deficiency drives pre-malignant transformation by reducing DNA repair capacity in concert with reprogramming the epigenome in human bronchial epithelial cells. DNA Repair 2019, 79, 1–9, doi:10.1016/j.dnarep.2019.04.006.
- Lee, H.S.; Choe, G.; Park, K.U.; Park, D.J.; Yang, H.K.; Lee, B.L.; Kim, W.H. Altered expression of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) during gastric carcinogenesis and its clinical implications on gastric cancer. Int. J. Oncol. 2007, 31, 859–866, doi:10.3892/ijo.31.4.859.
- He, S.-S.; Chen, Y.; Shen, X.-M.; Wang, H.-Z.; Sun, P.; Dong, J.; Guo, G.-F.; Chen, J.-G.; Xia, L.-P.; Hu, P.-L.; et al. DNA-dependent protein kinase catalytic subunit functions in metastasis and influences survival in advanced-stage laryngeal squamous cell carcinoma. J. Cancer 2017, 8, 2410–2416, doi:10.7150/jca.20069.
- Xing, J.; Wu, X.; Vaporciyan, A.A.; Spitz, M.R.; Gu, J. Prognostic significance ofataxia-telangiectasia mutated, DNA-dependent protein kinase catalytic subunit, and Ku heterodimeric regulatory complex 86-kD subunit expression in patients with nonsmall cell lung cancer. Cancer 2008, 112, 2756–2764, doi:10.1002/cncr.23533.
- Ren, F.; Yang, Z.-L.; Tan, X.; Liu, D.; Zou, Q.; Yuan, Y.; Li, J.; Liang, L.; Zeng, G.; Chen, S. DNA-PKcs and Ku70 are Predictive Markers for Poor Prognosis of Patients With Gall Bladder Malignancies. Appl. Immunohistochem. Mol. Morphol. 2014, 22, 741–747, doi:10.1097/pai.0000000000000017.
- Sun, G.; Yang, L.; Dong, C.; Ma, B.; Shan, M.; Ma, B. PRKDC regulates chemosensitivity and is a potential prognostic and predictive marker of response to adjuvant chemotherapy in breast cancer patients. Oncol. Rep. 2017, 37, 3536–3542, doi:10.3892/or.2017.5634.
- Zhang, X.; Wang, Y.; Ning, Y. Down-regulation of protein kinase, DNA-activated, catalytic polypeptide attenuates tumor progression and is an independent prognostic predictor of survival in prostate cancer. Urol. Oncol. Semin. Orig. Investig. 2017, 35, 111.e15–111.e23, doi:10.1016/j.urolonc.2016.10.012.
- Willoughby, C.E.; Jiang, Y.; Thomas, H.D.; Willmore, E.; Kyle, S.; Wittner, A.; Phillips, N.; Zhao, Y.; Tudhope, S.J.; Prendergast, L.; et al. Selective DNA-PKcs inhibition extends the therapeutic index of localized radiotherapy and chemotherapy. J. Clin. Investig. 2019, 130, 258–271, doi:10.1172/jci127483.
- Fok, J.H.L.; Ramos-Montoya, A.; Vazquez-Chantada, M.; Wijnhoven, P.W.G.; Follia, V.; James, N.; Farrington, P.M.; Karmokar, A.; Willis, S.E.; Cairns, J.; et al. AZD7648 is a potent and selective DNA-PK inhibitor that enhances radiation, chemotherapy and olaparib activity. Nat. Commun. 2019, 10, 1–15, doi:10.1038/s41467-019-12836-9.
- Zhang, J.; Jiang, H.; Xu, D.; Wu, W.; Chen, H.-D.; He, L. DNA-PKcs Mediates an Epithelial-Mesenchymal Transition Process Promoting Cutaneous Squamous Cell Carcinoma Invasion and Metastasis by Targeting The TGF-β1/Smad Signaling Pathway. Onco. Targets Ther. 2019, 12, 9395–9405, doi:10.2147/OTT.S205017.
- Liu, Y.; Efimova, E.V.; Ramamurthy, A.; Kron, S.J. Repair-independent functions of DNA-PKcs protect irradiated cells from mitotic slippage and accelerated senescence. J. Cell Sci. 2019, 132, jcs229385, doi:10.1242/jcs.229385.
- Tsai, A.K.; Khan, A.Y.; Worgo, C.E.; Wang, L.L.; Liang, Y.; Davila, E. A Multikinase and DNA-PK Inhibitor Combination Immunomodulates Melanomas, Suppresses Tumor Progression, and Enhances Immunotherapies. Cancer Immunol. Res. 2017, 5, 790–803, doi:10.1158/2326-6066.cir-17-0009.
- Dylgjeri, E.; McNair, C.; Goodwin, J.F.; Raymon, H.K.; McCue, P.A.; Shafi, A.A.; Leiby, B.E.; De Leeuw, R.; Kothari, V.; McCann, J.J.; et al. Pleiotropic Impact of DNA-PK in Cancer and Implications for Therapeutic Strategies. Clin. Cancer Res. 2019, 25, 5623–5637, doi:10.1158/1078-0432.ccr-18-2207.
- Puustinen, P.; Keldsbo, A.; Corcelle-Termeau, E.; Ngoei, K.; Sønder, S.L.; Farkas, T.; Andersen, K.K.; Oakhill, J.S.; Jäättelä, M. DNA-dependent protein kinase regulates lysosomal AMP-dependent protein kinase activation and autophagy. Autophagy 2020, 16, 1871–1888, doi:10.1080/15548627.2019.1710430.
- Zenke, F.T.; Zimmermann, A.; Sirrenberg, C.; Dahmen, H.; Kirkin, V.; Pehl, U.; Grombacher, T.; Wilm, C.; Fuchss, T.; Amendt, C.; et al. Pharmacologic Inhibitor of DNA-PK, M3814, Potentiates Radiotherapy and Regresses Human Tumors in Mouse Models. Mol. Cancer Ther. 2020, 19, 1091–1101, doi:10.1158/1535-7163.mct-19-0734.
- Leahy, J.J.; Golding, B.T.; Griffin, R.J.; Hardcastle, I.R.; Richardson, C.; Rigoreau, L.; Smith, G.C. Identification of a highly potent and selective DNA-dependent protein kinase (DNA-PK) inhibitor (NU7441) by screening of chromenone libraries. Bioorganic Med. Chem. Lett. 2004, 14, 6083–6087, doi:10.1016/j.bmcl.2004.09.060.
- Wang, C.; Tang, H.; Geng, A.; Dai, B.; Zhang, H.; Sun, X.; Chen, Y.; Qiao, Z.; Zhu, H.; Yang, J.; et al. Rational combination therapy for hepatocellular carcinoma with PARP1 and DNA-PK inhibitors. Proc. Natl. Acad. Sci. USA, 2020, 42, doi: 10.1073/pnas.2002917117.
- Bendell, J.C.; Shafique, M.R.; Perez, B.; Chennoufi, S.; Beier, F.; Trang, K.; Antonia, S.J. Phase 1, open-label, dose-escalation study of M3814 + avelumab ± radiotherapy (RT) in patients (pts) with advanced solid tumors. J. Clin. Oncol. 2019, 37, TPS3169, doi:10.1200/jco.2019.37.15_suppl.tps3169.
- Van Triest, B.; Damstrup, L.; Falkenius, J.; Budach, V.; Troost, E.; Samuels, M.; Debus, J.; Sørensen, M.M.; Berghoff, K.; Strotman, R.; et al. A phase Ia/Ib trial of the DNA-PK inhibitor M3814 in combination with radiotherapy (RT) in patients (pts) with advanced solid tumors: Dose-escalation results. J. Clin. Oncol. 2018, 36, 2518, doi:10.1200/jco.2018.36.15_suppl.2518.
- Sirbu, B.M.; Cortez, D. DNA Damage Response: Three Levels of DNA Repair Regulation. Cold Spring Harb. Perspect. Biol. 2013, 5, a012724, doi:10.1101/cshperspect.a012724.
- Jette, N.; Lees-Miller, S.P. The DNA-dependent protein kinase: A multifunctional protein kinase with roles in DNA double strand break repair and mitosis. Prog. Biophys. Mol. Biol. 2015, 117, 194–205, doi:10.1016/j.pbiomolbio.2014.12.003.
- Evert, M.; Frau, M.; Tomasi, M.; Latte, G.; Simile, M.; Seddaiu, M.; Zimmermann, A.; Ladu, S.; Staniscia, T.; Brozzetti, S.; et al. Deregulation of DNA-dependent protein kinase catalytic subunit contributes to human hepatocarcinogenesis development and has a putative prognostic value. Br. J. Cancer 2013, 109, 2654–2664, doi:10.1038/bjc.2013.606.
- Kothari, V.; Goodwin, J.F.; Zhao, S.G.; Drake, J.M.; Yin, Y.; Chang, S.L.; Evans, J.R.; Wilder-Romans, K.; Gabbara, K.; Dylgjeri, E.; et al. DNA-Dependent Protein Kinase Drives Prostate Cancer Progression through Transcriptional Regulation of the Wnt Signaling Pathway. Clin. Cancer Res. 2019, 25, 5608–5622, doi:10.1158/1078-0432.CCR-18-2387.
- Kotula, E.; Berthault, N.; Agrario, C.; Lienafa, M.-C.; Simon, A.; Dingli, F.; Loew, D.; Sibut, V.; Saule, S.; Dutreix, M. DNA-PKcs plays role in cancer metastasis through regulation of secreted proteins involved in migration and invasion. Cell Cycle 2015, 14, 1961–1972, doi:10.1080/15384101.2015.1026522.
- Wang, Y.; Xu, H.; Liu, T.; Huang, M.; Butter, P.-P.; Li, C.; Zhang, L.; Kao, G.D.; Gong, Y.; Maity, A.; et al. Temporal DNA-PK activation drives genomic instability and therapy resistance in glioma stem cells. JCI Insight 2018, 3, doi:10.1172/jci.insight.98096.
- Hu, H.; Gu, Y.; Qian, Y.; Hu, B.; Zhu, C.; Wang, G.; Li, J. DNA-PKcs is important for Akt activation and gemcitabine resistance in PANC-1 pancreatic cancer cells. Biochem. Biophys. Res. Commun. 2014, 452, 106–111, doi:10.1016/j.bbrc.2014.08.059.
- Yang, H.; Liang, S.-Q.; Xu, D.; Yang, Z.; Marti, T.M.; Gao, Y.; Kocher, G.J.; Zhao, H.; Schmid, R.A.; Peng, R.-W. HSP90/AXL/eIF4E-regulated unfolded protein response as an acquired vulnerability in drug-resistant KRAS-mutant lung cancer. Oncogenesis 2019, 8, 1–13, doi:10.1038/s41389-019-0158-7.
- Kitai, H.; Ebi, H.; Tomida, S.; Floros, K.V.; Kotani, H.; Adachi, Y.; Oizumi, S.; Nishimura, M.; Faber, A.C.; Yano, S. Epithelial-to-Mesenchymal Transition Defines Feedback Activation of Receptor Tyrosine Kinase Signaling Induced by MEK Inhibition in KRAS-Mutant Lung Cancer. Cancer Discov. 2016, 6, 754–69, doi:10.1158/2159-8290.CD-15-1377.
- Malta, T.M.; Sokolov, A.; Gentles, A.J.; Burzykowski, T.; Poisson, L.; Weinstein, J.N.; Kamińska, B.; Huelsken, J.; Omberg, L.; Gevaert, O.; et al. Machine Learning Identifies Stemness Features Associated with Oncogenic Dedifferentiation. Cell 2018, 173, 338–354.e15, doi:10.1016/j.cell.2018.03.034.
- Cheng, L.; Liu, Y.-Y.; Lu, P.-H.; Peng, Y.; Yuan, Q.; Gu, X.-S.; Jin, Y.; Chen, M.-B.; Bai, X.-M. Identification of DNA-PKcs as a primary resistance factor of TIC10 in hepatocellular carcinoma cells. Oncotarget 2017, 8, 28385–28394, doi:10.18632/oncotarget.16073.
- Javvadi, P.; Makino, H.; Das, A.K.; Lin, Y.-F.; Chen, D.J.; Chen, B.P.; Nirodi, C.S. Threonine 2609 Phosphorylation of the DNA-Dependent Protein Kinase Is a Critical Prerequisite for Epidermal Growth Factor Receptor-Mediated Radiation Resistance. Mol. Cancer Res. 2012, 10, 1359–1368, doi:10.1158/1541-7786.mcr-12-0482-t.
- Hanahan, D.; Weinberg, R.A. Hallmarks of Cancer: The Next Generation. Cell 2011, 144, 646–674, doi:10.1016/j.cell.2011.02.013.
- Faubert, B.; Solmonson, A.; DeBerardinis, J.K.Z.H.L.C.K.L.B.F.D.B.M.N.J.X.R.J. Metabolic reprogramming and cancer progression. Science 2020, 368, eaaw5473, doi:10.1126/science.aaw5473.
- Park, S.-J.; Gavrilova, O.; Brown, A.L.; Soto, J.E.; Bremner, S.; Kim, J.; Xu, X.; Yang, S.; Um, J.-H.; Koch, L.G.; et al. DNA-PK Promotes the Mitochondrial, Metabolic, and Physical Decline that Occurs During Aging. Cell Metab. 2017, 25, 1135–1146.e7, doi:10.1016/j.cmet.2017.04.008.
- Amatya, P.N.; Kim, H.-B.; Park, S.-J.; Youn, C.-K.; Hyun, J.-W.; Chang, I.-Y.; Lee, J.; You, H.J. A role of DNA-dependent protein kinase for the activation of AMP-activated protein kinase in response to glucose deprivation. Biochim. Biophys. Acta Bioenerg. 2012, 1823, 2099–2108, doi:10.1016/j.bbamcr.2012.08.022.
- Wong, R.H.; Chang, I.; Hudak, C.S.S.; Hyun, S.; Kwan, H.-Y.; Sul, H.S. A Role of DNA-PK for the Metabolic Gene Regulation in Response to Insulin. Cell 2009, 136, 1056–1072, doi:10.1016/j.cell.2008.12.040.
- Chung, J.H. The role of DNA ‐ PK in aging and energy metabolism. FEBS J. 2018, 285, 1959–1972, doi:10.1111/febs.14410.
- Miller, D.M.; Thomas, S.D.; Islam, A.; Muench, D.; Sedoris, K. c-Myc and Cancer Metabolism. Clin. Cancer Res. 2012, 18, 5546–5553, doi:10.1158/1078-0432.ccr-12-0977.
- Yan-Ying, H.; Yang, D.-Y.; Xu, Q.; Zhang, S.-M.; Huo, Y.-Y.; Shang, Z.-F.; Wang, Y.; Wu, D.-C.; Zhou, P. DNA-dependent protein kinase catalytic subunit modulates the stability of c-Myc oncoprotein. Mol. Cancer 2008, 7, 32, doi:10.1186/1476-4598-7-32.
- Guo, J.Y.; White, E. Autophagy, Metabolism, and Cancer. Cold Spring Harb. Symp. Quant. Biol. 2016, 81, 73–78, doi:10.1101/sqb.2016.81.030981.
- Daido, S.; Yamamoto, A.; Fujiwara, K.; Sawaya, R.; Kondo, S.; Kondo, Y. Inhibition of the DNA-Dependent Protein Kinase Catalytic Subunit Radiosensitizes Malignant Glioma Cells by Inducing Autophagy. Cancer Res. 2005, 65, 4368–4375, doi:10.1158/0008-5472.CAN-04-4202.
- Zhuang, W.; Li, B.; Long, L.; Chen, L.; Huang, Q.; Liang, Z. Knockdown of the DNA-dependent protein kinase catalytic subunit radiosensitizes glioma-initiating cells by inducing autophagy. Brain Res. 2011, 1371, 7–15, doi:10.1016/j.brainres.2010.11.044.
- Yu, L.; Shang, Z.-F.; Hsu, F.-M.; Zhang, Z.; Tumati, V.; Lin, Y.-F.; Chen, B.P.C.; Saha, D. NSCLC cells demonstrate differential mode of cell death in response to the combined treatment of radiation and a DNA-PKcs inhibitor. Oncotarget 2015, 6, 3848–3860, doi:10.18632/oncotarget.2975.
- Yu, L.; Tumati, V.; Tseng, S.-F.; Hsu, F.-M.; Kim, D.N.; Hong, D.; Hsieh, J.-T.; Jacobs, C.; Kapur, P.; Saha, D. DAB2IP Regulates Autophagy in Prostate Cancer in Response to Combined Treatment of Radiation and a DNA-PKcs Inhibitor. Neoplasia 2012, 14, 1203–IN36, doi:10.1593/neo.121310.
- Yang, H.; Liang, S.-Q.; Schmid, R.A.; Peng, R. New Horizons in KRAS-Mutant Lung Cancer: Dawn After Darkness. Front. Oncol. 2019, 9, 953, doi:10.3389/fonc.2019.00953.
- Björkman, A.; Du, L.; Felgentreff, K.; Rosner, C.; Kamdar, R.P.; Kokaraki, G.; Matsumoto, Y.; Davies, E.G.; Van Der Burg, M.; Notarangelo, L.D.; et al. DNA-PKcs Is Involved in Ig Class Switch Recombination in Human B Cells. J. Immunol. 2015, 195, 5608–5615, doi:10.4049/jimmunol.1501633.
- Rooney, S.; Alt, F.W.; Sekiguchi, J.; Manis, J.P. Artemis-independent functions of DNA-dependent protein kinase in Ig heavy chain class switch recombination and development. Proc. Natl. Acad. Sci. USA 2005, 102, 2471–2475.
- Crowe, J.L.; Wang, X.S.; Shao, Z.; Lee, B.J.; Estes, V.M.; Zha, S. DNA-PKcs phosphorylation at the T2609 cluster alters the repair pathway choice during immunoglobulin class switch recombination. Proc. Natl. Acad. Sci. USA 2020, 117, 22953–22961.
- Tan, K.T.; Yeh, C.-N.; Chang, Y.-C.; Cheng, J.-H.; Fang, W.-L.; Yeh, Y.-C.; Wang, Y.-C.; Hsu, D.S.-S.; Wu, C.-E.; Lai, J.-I.; et al. PRKDC: new biomarker and drug target for checkpoint blockade immunotherapy. J. Immunother. Cancer 2020, 8, e000485, doi:10.1136/jitc-2019-000485.
- Chen, Y.; Li, Y.; Guan, Y.; Huang, Y.; Lin, J.; Chen, L.; Li, J.; Chen, G.; Pan, L.K.; Xia, X.; et al. Prevalence of PRKDC mutations and association with response to immune checkpoint inhibitors in solid tumors. Mol. Oncol. 2020, 14, 2096–2110, doi:10.1002/1878-0261.12739.
- Chen, M.-H.; Tan, K.T.; Cheng, J.-H.; Fang, W.-L.; Yeh, Y.C.; Yeh, C.N.; Chang, Y.C.; Chen, S.-J.; Hsiao, M.; Chao, Y. PRKDC: A new candidate for checkpoint blockade immunotherapy? J. Clin. Oncol. 2017, 35, 3022, doi:10.1200/jco.2017.35.15_suppl.3022.
- Thorsson, V.; Gibbs, D.L.; Brown, S.; Wolf, D.; Bortone, D.S.; Yang, T.-H.O.; Porta-Pardo, E.; Gao, G.F.; Plaisier, C.L.; Eddy, J.A.; et al. The Immune Landscape of Cancer. Immunity 2018, 48, 812–830.e14, doi:10.1016/j.immuni.2018.03.023.
- Li, T.; Fu, J.; Zeng, Z.; Cohen, D.; Li, J.; Chen, Q.; Li, B.; Liu, X.S. TIMER2.0 for analysis of tumor-infiltrating immune cells. Nucleic Acids Res. 2020, 48, W509–W514, doi:10.1093/nar/gkaa407.
- Yin, X.; Liu, M.; Tian, Y.; Wang, J.; Xu, Y. Cryo-EM structure of human DNA-PK holoenzyme. Cell Res. 2017, 27, 1341–1350, doi:10.1038/cr.2017.110.
- Song, Z.; Xie, Y.; Guo, Z.; Han, Y.; Guan, H.; Liu, X.; Ma, T.; Zhou, P.-K. Genome-wide identification of DNA-PKcs-associated RNAs by RIP-Seq. Signal Transduct. Target. Ther. 2019, 4, 1–3, doi:10.1038/s41392-019-0057-6.
- Musiani, D.; Giambruno, R.; Massignani, E.; Ippolito, M.R.; Maniaci, M.; Jammula, S.; Manganaro, D.; Cuomo, A.; Nicosia, L.; Pasini, D.; et al. PRMT1 Is Recruited via DNA-PK to Chromatin Where It Sustains the Senescence-Associated Secretory Phenotype in Response to Cisplatin. Cell Rep. 2020, 30, 1208–1222.e9, doi:10.1016/j.celrep.2019.12.061.
- Zhang, Y.; He, Q.; Hu, Z.; Feng, Y.; Fan, L.; Tang, Z.; Yuan, J.; Shan, W.; Li, C.; Hu, X.; et al. Long noncoding RNA LINP1 regulates repair of DNA double-strand breaks in triple-negative breast cancer. Nat. Struct. Mol. Biol. 2016, 23, 522–530, doi:10.1038/nsmb.3211.
- Ting, N.S.Y.; Yu, Y.; Pohorelic, B.; Lees-Miller, S.P.; Beattie, T.L. Human Ku70/80 interacts directly with hTR, the RNA component of human telomerase. Nucleic Acids Res. 2005, 33, 2090–2098, doi:10.1093/nar/gki342.
- Peddi, P.; Loftin, C.W.; Dickey, J.S.; Hair, J.M.; Burns, K.J.; Aziz, K.; Francisco, D.C.; Panayiotidis, M.I.; Sedelnikova, O.A.; Bonner, W.M.; et al. DNA-PKcs deficiency leads to persistence of oxidatively induced clustered DNA lesions in human tumor cells. Free. Radic. Biol. Med. 2010, 48, 1435–1443, doi:10.1016/j.freeradbiomed.2010.02.033.
- Riabinska, A.; Daheim, M.; Herter-Sprie, G.S.; Winkler, J.; Fritz, C.; Hallek, M.; Thomas, R.K.; Kreuzer, K.-A.; Frenzel, L.P.; Monfared, P.; et al. Therapeutic Targeting of a Robust Non-Oncogene Addiction to PRKDC in ATM-Defective Tumors. Sci. Transl. Med. 2013, 5, 189ra78, doi:10.1126/scitranslmed.3005814.
- Sun, S.; Cheng, S.; Zhu, Y.; Zhang, P.; Liu, N.; Xu, T.; Sun, C.; Lv, Y. Identification of PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide) as an essential gene for colorectal cancer (CRCs) cells. Gene 2016, 584, 90–96, doi:10.1016/j.gene.2016.03.020.
- Zhang, Y.; Yang, W.; Wen, G.; Tang, H.; Wu, C.; Wu, Y.; Jing, Z.; Tang, M.; Liu, G.; Li, D.; et al. High expression of PRKDC promotes breast cancer cell growth via p38 MAPK signaling and is associated with poor survival. Mol. Genet. Genom. Med. 2019, 7, e908, doi:10.1002/mgg3.908.
- Sun, Q.; Guo, Y.; Liu, X.; Czauderna, F.; Carr, M.I.; Zenke, F.T.; Blaukat, A.; Vassilev, L.T. Therapeutic Implications of p53 Status on Cancer Cell Fate Following Exposure to Ionizing Radiation and the DNA-PK Inhibitor M3814. Mol. Cancer Res. 2019, 17, 2457–2468, doi:10.1158/1541-7786.mcr-19-0362.
- Dietlein, F.; Thelen, L.; Jokic, M.; Jachimowicz, R.D.; Ivan, L.; Knittel, G.; Leeser, U.; Van Oers, J.; Edelmann, W.; Heukamp, L.C.; et al. A Functional Cancer Genomics Screen Identifies a Druggable Synthetic Lethal Interaction between MSH3 and PRKDC. Cancer Discov. 2014, 4, 592–605, doi:10.1158/2159-8290.cd-13-0907.
- Yanai, M.; Makino, H.; Ping, B.; Takeda, K.; Tanaka, N.; Sakamoto, T.; Yamaguchi, K.; Kodani, M.; Yamasaki, A.; Igishi, T.; et al. DNA-PK Inhibition by NU7441 Enhances Chemosensitivity to Topoisomerase Inhibitor in Non-Small Cell Lung Carcinoma Cells by Blocking DNA Damage Repair. Yonago Acta Medica 2017, 60, 9–15.
- Kopa, P.; Macieja, A.; Gulbas, I.; Pastwa, E.; Poplawski, T. Inhibition of DNA-PK potentiates the synergistic effect of NK314 and etoposide combination on human glioblastoma cells. Mol. Biol. Rep. 2019, 47, 67–76, doi:10.1007/s11033-019-05105-x.
- Davidson, D.; Coulombe, Y.; Martinez-Marignac, V.L.; Amrein, L.; Grenier, J.; Hodkinson, K.; Masson, J.-Y.; Aloyz, R.; Panasci, L. Irinotecan and DNA-PKcs inhibitors synergize in killing of colon cancer cells. Investig. New Drugs 2012, 30, 1248–1256, doi:10.1007/s10637-010-9626-9.
- Xi, G.; A Hayes, E.; E Lewis, R.; Ichi, S.; Maniafarnell, B.; Shim, K.; Takao, T.; Allender, E.; Mayanil, C.S.; Tomita, T. CD133 and DNA-PK regulate MDR1 via the PI3K- or Akt-NF-κB pathway in multidrug-resistant glioblastoma cells in vitro. Oncogene 2015, 35, 241–250, doi:10.1038/onc.2015.78.
- Tu, Y.; Ji, C.; Yang, B.; Yang, Z.; Gu, H.; Lu, C.-C.; Wang, R.; Su, Z.-L.; Chen, B.; Sun, W.-L.; et al. DNA-dependent protein kinase catalytic subunit (DNA-PKcs)-SIN1 association mediates ultraviolet B (UVB)-induced Akt Ser-473 phosphorylation and skin cell survival. Mol. Cancer 2013, 12, 172, doi:10.1186/1476-4598-12-172.
- Stronach, E.A.; Chen, M.; Maginn, E.N.; Agarwal, R.; Mills, G.B.; Wasan, H.; Gabra, H. DNA-PK Mediates AKT Activation and Apoptosis Inhibition in Clinically Acquired Platinum Resistance. Neoplasia 2011, 13, 1069–IN35, doi:10.1593/neo.111032.
- Xu, W.; Yang, Z.; Lu, N. A new role for the PI3K/Akt signaling pathway in the epithelial-mesenchymal transition. Cell Adhes. Migr. 2015, 9, 317–324, doi:10.1080/19336918.2015.1016686.
- Lien, E.C.; Lyssiotis, C.A.; Cantley, L.C. Metabolic Reprogramming by the PI3K-Akt-mTOR Pathway in Cancer. Methods Mol. Biol. 2016, 207, 39–72, doi:10.1007/978-3-319-42118-6_3.
- Pastushenko, I.; Blanpain, C. EMT Transition States during Tumor Progression and Metastasis. Trends Cell Biol. 2019, 29, 212–226, doi:10.1016/j.tcb.2018.12.001.
- Woodbine, L.; Neal, J.A.; Sasi, N.-K.; Shimada, M.; Deem, K.; Coleman, H.; Dobyns, W.B.; Ogi, T.; Meek, K.; Davies, E.G.; et al. PRKDC mutations in a SCID patient with profound neurological abnormalities. J. Clin. Investig. 2013, 123, 2969–2980, doi:10.1172/jci67349.
- Menolfi, D.; Zha, S. ATM, ATR and DNA-PKcs kinases—the lessons from the mouse models: inhibition ≠ deletion. Cell Biosci. 2020, 10, 1–15, doi:10.1186/s13578-020-0376-x.
- Espejel, S.; Martin, M.; Klatt, P.; Martín-Caballero, J.; Flores, J.M.; Blasco, M.A. Shorter telomeres, accelerated ageing and increased lymphoma in DNA‐PKcs‐deficient mice. EMBO Rep. 2004, 5, 503–509, doi:10.1038/sj.embor.7400127.
- Kang, M.J.; Jung, S.-M.; Kim, M.J.; Bae, J.H.; Kim, H.B.; Kim, J.Y.; Park, S.J.; Song, H.S.; Kim, D.W.; Kang, C.D.; et al. DNA-dependent protein kinase is involved in heat shock protein-mediated accumulation of hypoxia-inducible factor-1α in hypoxic preconditioned HepG2 cells. FEBS J. 2008, 275, 5969–5981, doi:10.1111/j.1742-4658.2008.06725.x.
- Um, J.H.; Kang, C.D.; Bae, J.H.; Shin, G.G.; Kim, D.W.; Kim, D.W.; Chung, B.S.; Kim, S.H. Association of DNA-dependent protein kinase with hypoxia inducible factor-1 and its implication in resistance to anticancer drugs in hypoxic tumor cells. Exp. Mol. Med. 2004, 36, 233–242, doi:10.1038/emm.2004.32.
- Bouquet, F.; Ousset, M.; Biard, D.; Fallone, F.; Dauvillier, S.; Frit, P.; Salles, B.; Muller, C. A DNA-dependent stress response involving DNA-PK occurs in hypoxic cells and contributes to cellular adaptation to hypoxia. J. Cell Sci. 2011, 124, 1943–1951, doi:10.1242/jcs.078030.
- Lee, T.W.; Wong, W.W.; Dickson, B.D.; Lipert, B.; Cheng, G.J.; Hunter, F.W.; Hay, M.P.; Wilson, W.R. Radiosensitization of head and neck squamous cell carcinoma lines by DNA-PK inhibitors is more effective than PARP-1 inhibition and is enhanced by SLFN11 and hypoxia. Int. J. Radiat. Biol. 2019, 95, 1597–1612, doi:10.1080/09553002.2019.1664787.
- Wong, W.W.; Jackson, R.K.; Liew, L.P.; Dickson, B.D.; Cheng, G.J.; Lipert, B.; Gu, Y.; Hunter, F.W.; Wilson, W.R.; Hay, M.P. Hypoxia-selective radiosensitisation by SN38023, a bioreductive prodrug of DNA-dependent protein kinase inhibitor IC87361. Biochem. Pharmacol. 2019, 169, 113641, doi:10.1016/j.bcp.2019.113641.