Homologous Recombination Repair Deficiency
Homologous recombination repair deficiency (HRD) can be observed in virtually all cancer types. Cells possess a complex set of non-redundant and partially overlapping pathways to detect and repair DNA damage. In cancer, DNA damage repair (DDR) is frequently disrupted, leading to genomic instability. One of the pathways that is regularly altered in cancer is HR. HR is an important pathway for the repair of double-strand DNA breaks (DSBs) during the S and G2 phase of the cell cycle, i.e., after DNA replication has occurred. HR is considered a relatively error-free process because it uses an intact sister chromatid to guide DNA repair. HR deficiency (HRD) leads to enhanced reliance on alternative pathways involved in DSB repair, i.e., classical NHEJ, alternative end joining, and single-strand annealing. These pathways repair DSBs without a homologous DNA template, resulting in characteristic genomic scars across the genome.
1. How HRD Influences Antitumor Immunity
1.1. Tumor Mutational Burden and Neoantigen Load
To avoid autoimmunity, the immune system discriminates self-antigens from non-self-antigens. Due to mutations in protein-encoding genes, tumors may express aberrant antigens, known as neoantigens. These neoantigens may be recognized by the immune system as non-self, thereby generating an adaptive immune response, resulting in the selective elimination of cancer cells. HRD tumors exhibit a unique mutational signature, characterized by base-substitution signature 3 (enriched in C > G substitutions) and 8 (enriched in C > A substitutions) as well as an elevated number of small deletions (indels) with flanking microhomology (Figure 1) . Although tumor mutational burden (TMB) in HRD tumors is generally not as high as in MMR-deficient tumors, HRD tumors have consistently been described to have a higher TMB as compared to HR-proficient tumors . For instance, among two cohorts of breast cancer patients, the TMB was 2.0 to 2.6 times higher in patients with a BRCA1 or BRCA2 mutation as compared to those without a BRCA mutation . Across several types of cancers, high TMB has been associated with improved outcomes of checkpoint inhibitor therapy . A recent analysis among 1662 patients with various cancer types showed that high TMB, defined as the highest 20% of each tumor type, was associated with improved OS (hazard ratio = 0.61, p = 1.3 × 10–7) .
Figure 1. The genomic landscape of BRCA-mutated and BRCA-wildtype tumors. The depicted circos plots were generated using whole genome sequencing data of CPCT-02 study participants treated in the Radboudumc. Results of the CPCT-02 have previously been published elsewhere . The outer first circle shows the chromosomes. The darker areas represent large gaps in the human reference genome, i.e., regions of centromeres. The second circle shows all somatic single nucleotide variants (SNVs) across the genome. Tumor purity-adjusted allele frequencies are scaled from 0% to 100%. SNVs are colored according to the type of base change in concordance with coloring used in previous literature . Base substitutions that frequently occur in HRD are displayed in blue (C > A) and black (C > G). The third circle depicts short insertions (yellow) and deletions (red, indels). The fourth circle shows all copy number changes. Losses and gains are indicated in red and green, respectively. The scale ranges from 0 (complete loss) to 6 (high-level gains). Absolute copy numbers above 6 are indicated by a green dot on the diagram. The fifth circle represents the observed minor allele copy numbers. The scale ranges from 0 to 3, with losses (<1) shown in orange and gains (>1) shown in blue. The innermost circle displays the structural variants within or between the chromosomes. Translocations are indicated in blue, deletions are indicated in red, insertions are shown in yellow, tandem duplications are indicated in green, and inversions are shown in black. The figure shows that BRCA-mutated tumors generally have higher numbers of SNVs, small indels, deletions, and tandem duplications (the latter is only more frequent in BRCA1-mutated tumors).
Although a higher TMB increases the likelihood of the formation of neoantigens that are able to induce an immune response, not all non-synonymous mutations give rise to immunogenic neoantigens. Neoantigens are presented on the surface of cancer cells by major histocompatibility complex (MHC) molecules. The immunogenicity of neoantigens depends on its binding affinity to the patients’ MHC molecule. Several tools have been developed to predict neoantigen load, by inferring the MHC-peptide binding affinity from sequencing data. Like TMB, a high neoantigen load has been associated with checkpoint inhibitor efficacy . The neoantigen load has been described to be 2-fold to 3-fold higher in BRCA-mutated tumors as compared to BRCA wild-type tumors .
1.2. Copy Number Variations
The genomic instability of HRD tumors not only leads to a higher TMB, but also to large structural changes that result in a gain or loss of part of a chromosome. Research in breast and ovarian cancer identified three genomic signatures characteristic for HRD, which may result in copy number variations (CNVs). These include telomeric allelic imbalance (TAI) , loss of heterozygosity (LOH) , and large-scale state transitions (LST)  (Figure 2). Furthermore, the presence of ~10 kb duplications is specific for BRCA1-mutated tumors but not for other HRD tumors (Figure 1) .
Figure 2. Telomeric allelic imbalance (TAI), loss of heterozygosity (LOH), and large-scale state transitions (LSTs). (a) Genomic scars characteristic for homologous recombination repair deficiency (HRD) include TAI, LOH, and LSTs. Allelic imbalance is the imbalance in paternal and maternal alleles with or without changes in the overall copy number of that region. Characteristic for HRD is AI at the telomeric end of a chromosome (TAI). LOH refers to the situation where one of the two alleles that was originally present in the cell is lost. LSTs are defined as chromosomal breaks between adjacent regions of at least 10 mb. (b) CNV profile of an HRD tumor. The plot was generated using whole genome sequencing data of a CPCT-02 study participant treated in the Radboudumc . Dots represent regions of 10 mb. As LSTs lead to copy number changes, dots with a log ratio ≠ 0 indicate LSTs.
While little is known about the link between TAI, LOH, and LSTs and antitumor immunity, a relation has been suggested between immunity and the fraction of the genome altered by CNVs (CNV fraction). A large-scale analysis, including 9125 samples of 33 cancer types, demonstrated that the total number of TAI, LOH, and LSTs positively correlates with the CNV fraction, indicating that HRD tumors generally have a higher CNV fraction . A pan-cancer analysis of The Cancer Genome Atlas (TCGA) data showed that the CNV fraction negatively correlates with cytotoxic immune signatures, i.e., genes specific for cytotoxic CD8+ T cells and natural killer cells . The relation between the CNV fraction and the clinical outcome following treatment with anti-CTLA-4 was assessed in two independent cohorts of melanoma patients (n = 110 and n = 64). In both cohorts, a high CNV fraction was predictive of poor survival following treatment with anti-CTLA-4 (hazard ratio = 2.2, p = 0.0004 and hazard ratio = 2.3, p = 0.03, resp.) . Another study that assessed the relationship between the CNV fraction and response to anti-PD-(L)1 in 248 non-small cell lung cancer (NSCLC) patients showed an inverse relation between the CNV fraction and response to checkpoint inhibitors (p = 0.02) .
There is increasing evidence that CNVs play a critical role in tumorigenesis . Nevertheless, it is largely unclear why a high CNV fraction is associated with low cytotoxic immune signatures and a poor response to checkpoint inhibitors. It has been suggested that CNVs induce proteotoxic stress and, thereby, impair the signals needed to attract cytotoxic immune cells . An alternative hypothesis is that patients with a high CNV fraction more frequently harbor loss of tumor suppressor genes or amplification of driver genes that have been implicated in antitumor immunity, such as PTEN loss  or MYC amplification . In addition, loss of HLA loci, which encode MHC (or HLA) molecules, has been suggested to provide an advantage to cancers and allow for the outgrowth of subclones with an increased neoantigen load .
1.3. STING Pathway
Apart from the distinct genomic aberrations found in HRD tumors, the accumulation of DNA damage in these tumors may also affect their immunogenicity. Defects in the HR pathway have been associated with activation of the stimulator of interferon genes (STING) pathway in dendritic cells  and tumor cells . In this pathway, cytosolic DNA is sensed by cyclic GMP-AMP synthase (cGAS), leading to activation of STING and enhanced transcription of type I interferon (IFN) genes . Type I IFNs have immunostimulatory functions and play a role in promoting cross-presentation of antigens by dendritic cells, thereby, enhancing antigen-specific T cell responses . Preclinical research has shown that activation of the STING pathway by STING agonists induces immune-mediated tumor regression .
There is accumulating evidence that cytosolic DNA is increased in DDR-deficient cells and that this leads to altered STING pathway activity. Research in mice deficient for ATM and patients with congenital ATM deficiency demonstrated that loss of ATM, which is a DNA damage sensor, is associated with enhanced type I IFN production, which results from the accumulation of cytosolic DNA and activation of the STING pathway . In BRCA1-mutated breast cancer cells, increased cytosolic DNA levels and enhanced STING pathway activation have also been observed . Additionally, in HRD breast cancer cell lines and in vivo models, treatment with PARP inhibitors, which increases DSB formation, enhanced STING pathway activation and resulted in the recruitment of immune cells .
In summary, the slightly increased TMB and the STING-mediated upregulation of type I IFN genes observed in HRD tumors suggest that these tumors might be more immunogenic. The higher number of CNVs, on the other hand, might suppress antitumor immunity. This raises the question which of the mechanisms predominates in driving the immunogenicity of HRD tumors.
2. The Tumor Immune Microenvironment in BRCA-Inactivated Tumors
A comparison of the immune infiltrate between HRD and HR-proficient tumors could provide important insights into the immunogenic consequences of HRD. While a uniform definition of immunogenicity is lacking, a high number of tumor-infiltrating lymphocytes (TILs), especially of CD8+ T cells, is commonly considered indicative of immunogenicity . A more detailed description of the different immune cell subsets and checkpoint molecules discussed in this paragraph is given in the Box 1. In this section, we focus on differences in the immune infiltrate between BRCA-inactivated and BRCA wild-type tumors.
2.1. Breast Cancer
Several studies in breast cancer suggest an association between BRCA mutation status and increased immune cell infiltration, especially for the BRCA1-mutated tumors. Nolan and colleagues evaluated the presence of TILs in triple negative breast cancer (TNBC) patients with (n = 29) and without (n = 64) pathogenic germline BRCA1 variants. Higher numbers of TILs were observed in BRCA1-mutated tumors as compared to BRCA1 wild-type tumors. The immune infiltrate in BRCA1-mutated tumors consisted of cytotoxic (CD8+) and helper (CD4+) T cells, with a low frequency of regulatory T cells (Tregs) . In accordance with this, a large-scale analysis in 1269 breast cancer patients revealed that low protein expression of BRCA1 was associated with high numbers of CD8+ TILs as compared to patients with normal BRCA1 expression . While the previously mentioned studies focused on BRCA1-mutated tumors, others also took BRCA2 mutation status into account. Kraya and colleagues found that cytolytic activity, defined as the mean expression of PRF1 and GZMA, was higher in patients with a BRCA-mutated tumor (48 BRCA1-mutated, 41 BRCA2-mutated) compared to patients with an HR-proficient tumor (n = 652), with no difference between BRCA1-mutated tumors and BRCA2-mutated tumors . Wen and colleagues, on the other hand, showed that only pathogenic BRCA1 but not BRCA2 variants were associated with a higher number of activated CD4+ and CD8+ T cells using transcriptome data of the Wellcome Sanger Institute and TCGA (n = 1418, 78 BRCA1-mutated and 53 BRCA2-mutated) .
While several studies suggest that BRCA1-mutated breast cancers have increased immune cell infiltration, there are also numerous studies that did not find any association between BRCA mutation status and immune cell infiltration . Further complicating the interpretation of the results, a recent study indicates that BRCA1-mutated tumors (n = 17) have a more immunosuppressed tumor microenvironment as compared to BRCA1 wild-type tumors, as evidenced by higher expression of immunoregulatory and suppressive genes . Interestingly, this was not the case for BRCA2-mutated tumors (n = 18). The authors observed lower numbers of SNVs and indels and higher CNV fractions in BRCA1-mutated tumors as compared to BRCA2-mutated tumors and suggest that these genomic differences may contribute to the observed differences in immunogenicity.
2.2. Ovarian Cancer
Most studies in ovarian cancer have reported increased TILs and immune checkpoint expression in BRCA-mutated tumors. In a cohort of 53 patients with serous ovarian cancer (29 BRCA1-mutated, 8 BRCA2-mutated, 16 HR-proficient), BRCA-mutated tumors exhibited increased CD3+ and CD8+ T cells as compared to HR-proficient tumors. PD-1 and PD-L1 expression on tumor-infiltrating immune cells was also higher in BRCA-mutated tumors, but no significant difference was observed in PD-L1 expression on tumor cells or the number of B cells . In line with these findings, a study in 40 patients, including 18 patients with a BRCA1-mutated tumor (n = 9) or a tumor with epigenetic loss of BRCA1 (n = 9), demonstrated that intraepithelial CD8+ TILs were more frequently observed in tumors with BRCA1 abnormalities (94.4% vs. 57.9%) . Additionally, a study among 158 ovarian cancer patients (37 BRCA-mutated, 121 BRCA-wildtype) showed that BRCA-mutated tumors had significantly higher levels of PD-1 and PD-L1 mRNA as compared to BRCA wild-type tumors . Finally, in a study among 103 patients with serous ovarian cancer (21 BRCA1-mutated, 10 BRCA2-mutated, 21 BRCA1 methylation, and 51 no BRCA loss), BRCA-mutated tumors tended to be more frequently infiltrated by CD8+ T cells (92.9%) as compared to tumors with BRCA1 methylation (76.2%) or no BRCA loss (73.9%, p = 0.057) . In contrast to the breast cancer studies that are described above, the ovarian cancer studies analyzed BRCA1-mutated and BRCA2-mutated tumors as a single group, making it impossible to evaluate the contribution of the individual genes.
Like in breast cancer, there are also a few studies in ovarian cancer that do not support an association between BRCA-inactivation and immune cell infiltration. In an immunohistochemistry study including 48 patients with serous ovarian cancer and known germline BRCA mutation status (4 BRCA1-mutated, 8 BRCA2-mutated, 36 BRCA-wildtype), no association was found between germline BRCA mutation status and the infiltration of CD8+ T cells or Tregs or checkpoint expression (PD-L1 or LAG-3) . Furthermore, analyses of transcriptome data of the TCGA yielded conflicting results .
2.3. Prostate Cancer
In an attempt to better understand the impact of BRCA2 mutations on the immune phenotype of prostate cancer, Jenzer and colleagues performed immunohistochemistry and T-cell receptor (TCR) sequencing in nine BRCA2-mutated and nine BRCA wild-type, hormone-sensitive prostate cancers. No difference was observed in the number of T cell clones or TCR clonality. In BRCA2-mutated tumors, however, the ratio between intratumoral and stromal CD4+ T cells, CD8+ T cells and Tregs was higher as compared to BRCA2 wild-type tumors. Although the location of the T cells does not inform us about the antitumor activity of these cells, the closer proximity to the tumor cells does suggest a more active immune response .
Box 1. Immune Cell Subsets and Immune Checkpoints.
T cells are key players in antitumor immunity. They are able to selectively target cancer cells following recognition of non-self-antigens. T cells, characterized by the expression of CD3, can be subdivided into cytolytic T cells (CD8+), helper T cells (CD4+), and regulatory T cells (CD4+FoxP3+). While cytolytic T cells and helper T cells play an important role in tumor immunosurveillance, regulatory T cells suppress antitumor immunity. Studies in various cancer types indicate that high intratumoral CD8+ T cell density is associated with favorable outcomes to checkpoint inhibitor therapy . Nevertheless, the sole presence of CD8+ T cells does not necessarily indicate an active immune response. Immune activity can be inhibited by a lack of antigen presentation or by the presence of immune suppressive cells, cytokines, or inhibitory checkpoint molecules.
B cells play a major role in antibody-mediated immunity. Although their role in antitumor immunity is not completely understood, recent data suggest that B cells play a role in antitumor immunity and promote checkpoint inhibitor efficacy .
Natural killer cells are innate immune cells with a cytolytic function.
Checkpoint molecules play an important role in regulating immune responses. PD-L1, PD-1, and LAG-3 are all inhibitory checkpoint molecules. Activation of these checkpoints suppresses immune cell activation. In some cancer types, PD-L1 expression is associated with a response to PD-(L)1 inhibitors . In NSCLC and urothelial cancer, PD-L1 expression is used for treatment stratification.
Although several studies in various cancer types indicate that BRCA-inactivated tumors have more dense immune infiltrates, current data is inconclusive. There are several possible explanations for these heterogeneous results. First, study results might have been biased due to the presence of sporadic cancers in the BRCA-mutated group. In most studies the BRCA-mutated group was not limited to patients with biallelic BRCA inactivation. Across cancer types, only 89% of patients with a germline BRCA1 variant and 79% of patients with a BRCA2 variant have a tumor with complete loss of the wild-type allele . Besides the presence of sporadic cancers in the BRCA-mutated group, there might also have been HRD tumors in the BRCA-wildtype group as HRD can also arise from mutations in other HR genes or promoter hypermethylation . Recently, genome-wide, mutational scar-based scores have been developed for the assessment of HRD, such as HRDetect  and CHORD . Up to 45% of cancer patients with an HRD tumor according to CHORD do not have an event in BRCA1 or BRCA2 . Unfortunately, currently available data on the association of HRD and immune cell infiltration has focused on the BRCA mutation status and did not take genome-wide HRD signatures into account. Finally, an explanation for the inconsistent results might be the heterogeneity of HRD tumors. The immunogenicity of the HRD tumor might differ depending on the degree of genomic instability and the genomic regions where alterations have occurred. It is conceivable, for example, that amplification or loss of driver genes involved in immune suppression might hamper antitumor immunity despite higher TMB and STING pathway activation. While evidence on this subject is currently limited, it is plausible that only those tumors with a high number of SNVs and indels and a low CNV fraction are more immunogenic. In support of this, a study in breast cancer patients described a negative association between T cell infiltration and the degree of LOH, TAI, and LSTs within the BRCA-mutated subgroup .
3. Checkpoint Inhibitor Therapy in BRCA-Inactivated Tumors
3.1. Tumor Types with Low Sensitivity to Checkpoint Inhibitor Monotherapy
Although checkpoint inhibitors have greatly improved clinical outcomes in some cancers, checkpoint inhibitors have had limited success in many other tumor types so far. These tumor types include breast cancer, ovarian cancer, and prostate cancer, i.e., tumor types where HRD occurs rather frequently. Although checkpoint inhibitors are not beneficial for the entire group of breast cancer, ovarian cancer, or prostate cancer patients, selected subgroups may benefit. HRD has been suggested to function as a biomarker to select patients for checkpoint inhibitor therapy.
3.1.1. Breast Cancer
Most research on checkpoint inhibitors in breast cancer has focused on patients with TNBC, a subgroup that is enriched for BRCA1 mutations . Checkpoint inhibitors have shown modest activity in breast cancer, when used as a single agent . Nevertheless, the combination of the PD-L1 inhibitor atezolizumab with the chemotherapeutic agent nab-paclitaxel has been shown to improve median OS as compared with nab-paclitaxel alone in PD-L1+ TNBC (25.0 to 15.5 months) . Preclinical studies suggest that BRCA2-mutated but not BRCA1-mutated breast cancers are responsive to treatment with checkpoint inhibitor monotherapy . However, clinical studies supporting this are lacking. Data from clinical trials suggest that BRCA-mutated TNBCs are not more susceptible to treatment with atezolizumab plus nab-paclitaxel . Furthermore, BRCA1-like, genomic copy number profiles appear to be negatively associated with response to PD-1 blockade in TNBC  (Table 1).
Table 1. The efficacy of checkpoint inhibitors in HRD tumors.
|Reference||Tumor Type||N||Genes||Treatment||Results 1|
|||TNBC||612||89||Pathogenic germline or somatic BRCA1/2 variants, zygosity status not assessed||Atezolizumab + nab-paclitaxel||PFS: hazard ratio 1.07, 95% CI 0.77–1.49
OS: hazard ratio 1.07, 95% CI 0.71–1.62
|||TNBC||49||25||BRCA1-like genomic copy number profiles||Nivolumab with or without induction chemotherapy or irradiation||Lower ORR in BRCA1-like patients (p < 0.05)|
|||Ovarian cancer||46||8||Pathogenic germline BRCA1/2 variants, zygosity status not assessed||Avelumab||ORR: 12.5% (1/8) in BRCA-mut vs 7.9% (3/38) in BRCA-WT|
|||Ovarian or fallopian tubal cancer||6||6||Pathogenic germline BRCA1/2 variants, zygosity status not assessed||Nivolumab||ORR: 76% (3/6 CR, 1/6 PR, 2/6 PD)|
|||Ovarian or uterine cancer||25||2||Pathogenic germline BRCA1 variants||Atezolizumab||ORR: Both had PD|
|||CRPC||153||19||Pathogenic homozygous BRCA1/2 or ATM aberrations||Pembrolizumab||ORR: 11% (2/19) in patients with BRCA1/2 or ATM aberrations and 3% (4/124) in patients without HR aberrations|
|||CRPC||28||5||Pathogenic homozygous aberrations in BRCA2, ATM, CDK12, or FANCA||Ipilimumab + nivolumab||ORR: 50% (3/6) in DDR-mut vs 22.6% (7/31) in DDR-WT.
Of note, responding patients in the DDR group had mutations in BRCA2 or FANCA
|||CRPC with AR-V7 expression||15||6||Pathogenic mutations in BRCA2 (3), ATM (2), ERCC4 (1)2, LOH in two BRCA2-mut patients||Ipilimumab + nivolumab||ORR: 40% (2/5) in DDR-mut vs 0% (0/3) in DDR-WT (p = 0.46)
PSA response: 33% (2/6) vs 0% (0/9) (p = 014)
PFS: hazard ratio = 0.31, 95% CI 0.10–0.92, p = 0.01
OS: hazard ratio = 0.41, 95% CI 0.14–1.21, p = 0.1
|||Urothelial cancer||60||15||Pathogenic alterations in BRCA1/2 (3) and other DDR genes (12; ATM, POLE, ERCC2, CHEK2, FANCA, and MSH2, MSH6). Zygosity status n/a||Anti-PD-(L)1||ORR: 80% (12/15) and 19% (6/32) in patients with deleterious DDR alterations and no DDR alterations, resp.
PFS: Median PFS NR3 and 2.9 months, resp
|||NSCLC||44||9||BRCA1/2 mutations. Zygosity status and pathogenicity n/a||Anti-PD-(L)1||10% and 29% of patients with and without durable benefit resp, harbored a mutation in BRCA1/2|
|||Renal cell carcinoma||34||12||BRCA1/2 mutations. Zygosity status and pathogenicity n/a||Anti-PD-1 alone (32) or combined with anti-CTLA-4 (2)||38% (6/16) of patients with disease control vs. 33% (6/18) of patients with PD had a mutation in an BRCA1/2|
|||Metastatic melanoma||38||7||BRCA2 mutations. Zygosity status and pathogenicity n/a||Anti-PD-1||BRCA2 mutation in 28% (6/21) of responders vs. 6% (1/17) of non-responders|
|||Various solid tumors||1661||335||ARID1 A, BLM, BRCA2, MRE11, NBN, RAD50, RAD51/B/D, RAD52, RAD54 L, XRCC2
Zygosity status and pathogenicity n/a
|Anti-CTLA-4 (9%), anti-PD-(L)1 (76%), or both (16%)||OS:
Median OS 41 months in HR-mut vs 16 months in HR-WT
Adj hazard ratio4 = 1.39, 95% CI 1.15–1.70, p = 0.022
|||Various tumors||2185||95||Pathogenic somatic or germline BRCA1 (28) or BRCA2 (67) mutations. Zygosity statis n/a||Anti-PD-(L)1, CTLA-4 or a combination||OS BRCA1:
Hazard ratio 0.76, 95% CI 0.48–1.54, p = 0.45
Hazard ratio 0.48, 95% CI 0.29–0.80
Adj hazard ratio5 = 0.50, 95% CI 0.30–0.83, p = 0.008
1 A hazard ratio <1 indicates better outcomes in patients with HRD tumors. 2 ERCC4 is involved in nucleotide excision repair. 3 Median follow up was 19.6 months. 4 Adjusted for high TMB, type of ICI administered, and tumor type. 5 Adjusted for TMB and cancer type. Abbreviations: adj = adjusted; AR-V7 = androgen receptor variant 7; 95% CI = 95% confidence interval; CRPC = castrate-resistant prostate cancer; DDR = DNA damage repair; HR = homologous recombination; LOH = loss of heterozygosity, mut = mutated; NSCLC = non-small cell lung cancer; ORR = objective response rate; OS = overall survival; PFS = progression-free survival; PD-1 = programmed cell death protein-1; PD-L1 = programmed cell death ligand 1; TNBC = triple negative breast cancer; WT = wild type.
3.1.2. Ovarian Cancer
Clinical trials on the efficacy of single-agent checkpoint inhibitor therapy in ovarian cancer have reported response rates around 10% . Due to these low response rates, checkpoint inhibitors have not (yet) been approved for the treatment of ovarian cancer, apart from the subset of patients with MMR-deficient tumors . Data on the efficacy of checkpoint inhibitors in HRD ovarian cancer is limited. A phase Ib trial on the efficacy of PD-L1 inhibitor avelumab reported only one objective response among eight patients with a pathogenic germline BRCA variant (12.5%). This response rate was very similar to that observed in BRCA-wildtype patients (7.9%) . A case series, on the other hand, described very promising responses to PD-1 inhibitor nivolumab among six patients with germline BRCA mutations and recurrent ovarian (n = 5) or fallopian tube (n = 1) cancer. Four out of six patients achieved an objective response, including three complete responses . Although it is possible that checkpoint inhibitors are more effective in patients with HRD ovarian cancer, the low response rates to checkpoint inhibitors together with the high frequency of HRD in ovarian cancer (up to 50%) indicates that checkpoint inhibitors are not effective in all patients with HRD ovarian cancer .
3.1.3. Prostate Cancer
Checkpoint inhibitor monotherapy has not been able to improve the clinical outcome in unselected patients with castrate-resistant prostate cancer (CRPC). Only 3–5% of CRPC patients achieve an objective response to anti-PD-1 . The response rate to combination therapy with ipilimumab and nivolumab appears to be higher. Yet, still only 10–26% of patients achieve an objective response . Exploratory biomarker analyses in clinical trials have suggested that HRD tumors might be more sensitive to checkpoint inhibitors. In the KEYNOTE-199, the objective response rate to pembrolizumab was 11% in patients with BRCA1-mutated, BRCA2-mutated, or ATM-mutated tumors and 3% in patients without mutations in HR-related genes . In the CheckMate 650, 50% of patients with an HRD tumor and only 22.6% of patients with HR-proficient tumors responded to combination therapy . Importantly, in the latter study, the authors used a broad definition of HRD, including not only BRCA, but also ATM, CDK12, and FANCA alterations. Only tumors with BRCA2 and FANCA mutations responded to therapy. Although promising, one should keep in mind that the number of patients in this trial were low (Table 1).
3.2. Tumor Types Responsive to Checkpoint Inhibitor Monotherapy
Biomarkers that enrich for response to checkpoint inhibitors may also have great utility in tumor types where checkpoint inhibitors are already part of standard care. In these tumor types, biomarkers may guide the treatment sequence and may help decide between checkpoint inhibitors monotherapy or combinational treatment strategies. Therefore, it is important to know how HRD affects checkpoint inhibitor sensitivity in these tumors.
3.2.1. Urothelial Cancer
In urothelial cancer, PD-(L)1 inhibitors are currently mostly used as second-line treatment for patients with metastatic disease who progressed on platinum-based chemotherapy . Recent trials investigating the efficacy of checkpoint inhibition in the first-line setting showed no survival benefit for checkpoint inhibitors over chemotherapy in the overall population . Nevertheless, first-line therapy with checkpoint inhibitors may be very effective in selected subgroups. A study of 60 patients with advanced urothelial cancer showed that 80% of patients who had tumors with a deleterious DDR alteration had an objective response to anti-PD-(L)1, whereas responses were seen in only 18.8% of patients without DDR alterations. Importantly, only three out of fifteen patients with deleterious DDR alterations harbored tumor mutations in BRCA1 or BRCA2 and no information is provided on the responses of these three patients. Other DDR mutations included ATM, POLE, ERCC2, FANCA, and MSH6 .
3.2.2. Other Cancer Types
The incidence of HRD in other tumor types where checkpoint inhibitors are part of standard care is very low. This includes melanoma, non-small cell lung cancer, and renal cell carcinoma . There have been a few reports on the association between BRCA mutations and checkpoint inhibitor efficacy in these tumors. Nevertheless, none of these studies reported on the zygosity status or pathogenicity of the identified mutations, making it difficult to interpret the results. These data are summarized in Table 1.
3.3. Pan-Cancer Analyses
A recent, large-scale, pan-cancer analysis in 1661 patients treated with checkpoint inhibitors demonstrated a significantly longer OS in patients with tumors with a mutation in an HR-related gene . Patients were treated with antibodies targeting CTLA-4 (9%), PD-(L)1 (76%), or both (16%). The authors distinguished between HR-related genes (ARID1A, BLM, BRCA2, MRE11, NBN, RAD50, RAD51/B/D, RAD52, RAD54L, XRCC2) and DNA checkpoints (including, among others, BRCA1, ATM, CHEK1, and CHEK2). Patients with tumor mutations in HR-related genes had significantly longer OS as compared to those without these mutations, independent of tumor type or TMB (41 months vs. 16 months, adjusted hazard ratio = 1.39, 95% CI 1.15–1.70, p < 0.001). In contrast to the HR-related genes, the DNA checkpoints were not associated with OS after adjustment for TMB and tumor type. The most frequently mutated HR-related genes were ARID1A (11.4%) and BRCA2 (5.6%). Mutations in BRCA2 as well as most other HR-related genes with an incidence of least 1% (ARID1A, RAD50, RAD51B, and MRE11) were individually associated with longer OS. In a cohort of patients not treated with checkpoint inhibitors, mutations in HR-related genes were associated with worse OS (HR 0.86, 95% CI 0.78–0.95, p = 0.003), suggesting that mutations in the designated HR-related genes have predictive value for response to checkpoint inhibitors rather than a prognostic value. Despite the retrospective character, the broad definition of HR-related genes, and the fact that the observed non-synonymous mutations were not assessed for their functional effects, this large scale analysis supports the idea that mutations in BRCA2 and other genes with a direct or indirect role in HR render tumors more susceptible for treatment with checkpoint inhibitors.
In line with these findings, another large study of 2185 patients with various cancer types also suggests higher sensitivity of BRCA2-mutated tumors to checkpoint inhibitors. Included patients were treated with anti-PD-(L)1, CTLA-4, or a combination of both. In total, 67 patients harbored a pathogenic germline or somatic variant in BRCA2 and 28 in BRCA1. Zygosity status was not assessed. In univariate analysis, BRCA2 but not BRCA1 mutations were associated with improved OS after checkpoint inhibitor therapy. The correlation between BRCA2 mutations and OS remained significant after controlling for tumor type and TMB (HR 0.50, 95% CI 0.30 – 0.83, p = 0.008). It is difficult to make a direct comparison between BRCA2-mutated and BRCA1-mutated tumors as the distribution of these mutations differs across cancer types and the correlation between the BRCA1 mutation status and OS was not controlled for tumor type. Nevertheless, the data suggest that patients with BRCA2-mutated tumors are more susceptible for treatment with checkpoint inhibitors.
There is evidence from two large pan-cancer analyses suggesting that checkpoint inhibitors are more effective in patients with BRCA2-mutated tumors. Data from other studies is limited by the small sample size, the lack of information on the pathogenicity of the identified mutations and zygosity status, and/or the broad definition of HR-related genes. All clinical data is summarized in Table 1. Prospective studies are needed to validate the findings of the two large pan-cancer trials and to provide more insight into HRD-associated hallmarks associated with responses to checkpoint inhibitors. As evident from the low response rates to checkpoint inhibitors in ovarian cancer, where HRD occurs in up to 50% of patients, it is clear that not all patients with HRD will respond to checkpoint inhibitors. Additional factors, such as the presence of a BRCA1-type or BRCA2-type HRD signature, the TMB, and the CNV fraction, might influence sensitivity to checkpoint inhibitors in these tumors. Phase II trials in patients with advanced solid tumors (ClinicalTrials.gov Identifier: NCT03428802) and metastatic CRPC (ClinicalTrials.gov Identifier: NCT04717154) have recently been initiated to study the efficacy of checkpoint inhibitors in HRD tumors. If (a subset) of HRD tumors prove to be more sensitive to checkpoint inhibitor therapy, this will have important implications for treating patients with HRD tumors.
The entry is from 10.3390/cancers13092249
- Nguyen, L.; Martens, J.W.; Van Hoeck, A.; Cuppen, E. Pan-cancer landscape of homologous recombination deficiency. Nat. Commun. 2020, 11, 5584.
- Davies, H.; Glodzik, D.; Morganella, S.; Yates, L.R.; Staaf, J.; Zou, X.; Ramakrishna, M.; Martin, S.; Boyault, S.; Sieuwerts, A.M.; et al. HRDetect is a predictor of BRCA1 and BRCA2 deficiency based on mutational signatures. Nat. Med. 2017, 23, 517–525.
- Birkbak, N.J.; Kochupurakkal, B.; Izarzugaza, J.M.G.; Eklund, A.C.; Li, Y.; Liu, J.; Szallasi, Z.; Matulonis, U.A.; Richardson, A.L.; Iglehart, J.D.; et al. Tumor Mutation Burden Forecasts Outcome in Ovarian Cancer with BRCA1 or BRCA2 Mutations. PLoS ONE 2013, 8, e80023.
- Przybytkowski, E.; Davis, T.; Hosny, A.; Eismann, J.; Matulonis, U.A.; Wulf, G.M.; Nabavi, S. An immune-centric exploration of BRCA1 and BRCA2 germline mutation related breast and ovarian cancers. BMC Cancer 2020, 20, 197.
- Van Dessel, L.F.; van Riet, J.; Smits, M.; Zhu, Y.; Hamberg, P.; van der Heijden, M.S.; Bergman, A.M.; van Oort, I.M.; de Wit, R.; Voest, E.E.; et al. The genomic landscape of metastatic castration-resistant prostate cancers reveals multiple distinct genotypes with potential clinical impact. Nat. Commun. 2019, 10, 5251.
- Nolan, E.; Savas, P.; Policheni, A.N.; Darcy, P.K.; Vaillant, F.; Mintoff, C.P.; Dushyanthen, S.; Mansour, M.; Pang, J.M.B.; Fox, S.B.; et al. Combined immune checkpoint blockade as a therapeutic strategy for BRCA1-mutated breast cancer. Sci. Transl. Med. 2017, 9, eaal4922.
- Wen, W.X.; Leong, C.O. Association of BRCA1- And BRCA2-deficiency with mutation burden, expression of PD-L1/ PD-1, immune infiltrates, and T cell-inflamed signature in breast cancer. PLoS ONE 2019, 14, e0215381.
- Kraya, A.A.; Maxwell, K.N.; Wubbenhorst, B.; Wenz, B.M.; Pluta, J.; Rech, A.J.; Dorfman, L.M.; Lunceford, N.; Barrett, A.; Mitra, N.; et al. Genomic signatures predict the immunogenicity of BRCA-deficient breast cancer. Clin. Cancer Res. 2019, 25, 4363–4374.
- Li, H.; Wu, L.; Shou, T.; Jiang, B.; Zhuang, L.; Li, K.; Tan, X.; Guo, C.; Guo, W.; Guan, Y.; et al. Abstract 1369: Analysis of association between homologous recombination deficiency and tumor mutational burden in solid tumors. In Proceedings of the Cancer Research, American Association for Cancer Research (AACR), Chicago, IL, USA, 14–18 April 2018; Volume 78, p. 1369.
- Chae, Y.K.; Anker, J.F.; Bais, P.; Namburi, S.; Giles, F.J.; Chuang, J.H. Mutations in DNA repair genes are associated with increased neo-antigen load and activated T cell infiltration in lung adenocarcinoma. Oncotarget 2018, 9, 7949–7960.
- Johnson, D.B.; Frampton, G.M.; Rioth, M.J.; Yusko, E.; Xu, Y.; Guo, X.; Ennis, R.C.; Fabrizio, D.; Chalmers, Z.R.; Greenbowe, J.; et al. Targeted next generation sequencing identifies markers of response to PD-1 blockade. Cancer Immunol. Res. 2016, 4, 959–967.
- Rizvi, N.A.; Hellmann, M.D.; Snyder, A.; Kvistborg, P.; Makarov, V.; Havel, J.J.; Lee, W.; Yuan, J.; Wong, P.; Ho, T.S.; et al. Mutational landscape determines sensitivity to PD-1 blockade in non-small cell lung cancer. Science 2015, 348, 124–128.
- Goodman, A.M.; Kato, S.; Bazhenova, L.; Patel, S.P.; Frampton, G.M.; Miller, V.; Stephens, P.J.; Daniels, G.A.; Kurzrock, R. Tumor Mutational Burden as an Independent Predictor of Response to Immunotherapy in Diverse Cancers. Mol. Cancer Ther. 2017, 16, 2598–2608.
- Samstein, R.M.; Lee, C.-H.; Shoushtari, A.N.; Hellmann, M.D.; Shen, R.; Janjigian, Y.Y.; Barron, D.A.; Zehir, A.; Jordan, E.J.; Omuro, A.; et al. Tumor mutational load predicts survival after immunotherapy across multiple cancer types. Nat. Genet. 2019, 51, 202–206.
- Van Allen, E.M.; Miao, D.; Schilling, B.; Shukla, S.A.; Blank, C.; Zimmer, L.; Sucker, A.; Hillen, U.; Geukes Foppen, M.H.; Goldinger, S.M.; et al. Genomic correlates of response to CTLA-4 blockade in metastatic melanoma. Science 2015, 350, 207–211.
- Le, D.T.; Uram, J.N.; Wang, H.; Bartlett, B.R.; Kemberling, H.; Eyring, A.D.; Skora, A.D.; Luber, B.S.; Azad, N.S.; Laheru, D.; et al. PD-1 Blockade in Tumors with Mismatch-Repair Deficiency. N. Engl. J. Med. 2015, 372, 2509–2520.
- Priestley, P.; Baber, J.; Lolkema, M.P.; Steeghs, N.; de Bruijn, E.; Shale, C.; Duyvesteyn, K.; Haidari, S.; van Hoeck, A.; Onstenk, W.; et al. Pan-cancer whole-genome analyses of metastatic solid tumours. Nature 2019, 575, 210–216.
- Alexandrov, L.B.; Nik-Zainal, S.; Wedge, D.C.; Aparicio, S.A.J.R.; Behjati, S.; Biankin, A.V.; Bignell, G.R.; Bolli, N.; Borg, A.; Børresen-Dale, A.L.; et al. Signatures of mutational processes in human cancer. Nature 2013, 500, 415–421.
- Birkbak, N.J.; Wang, Z.C.; Kim, J.Y.; Eklund, A.C.; Li, Q.; Tian, R.; Bowman-Colin, C.; Li, Y.; Greene-Colozzi, A.; Dirk Iglehart, J.; et al. Telomeric allelic imbalance indicates defective DNA repair and sensitivity to DNA-damaging agents. Cancer Discov. 2012, 2, 366–375.
- Abkevich, V.; Timms, K.M.; Hennessy, B.T.; Potter, J.; Carey, M.S.; Meyer, L.A.; Smith-Mccune, K.; Broaddus, R.; Lu, K.H.; Chen, J.; et al. Patterns of genomic loss of heterozygosity predict homologous recombination repair defects in epithelial ovarian cancer. Br. J. Cancer 2012, 107, 1776–1782.
- Popova, T.; Manié, E.; Rieunier, G.; Caux-Moncoutier, V.; Tirapo, C.; Dubois, T.; Delattre, O.; Sigal-Zafrani, B.; Bollet, M.; Longy, M.; et al. Ploidy and large-scale genomic instability consistently identify basal-like breast carcinomas with BRCA1/2 inactivation. Cancer Res. 2012, 72, 5454–5462.
- Willis, N.A.; Frock, R.L.; Menghi, F.; Duffey, E.E.; Panday, A.; Camacho, V.; Hasty, E.P.; Liu, E.T.; Alt, F.W.; Scully, R. Mechanism of tandem duplication formation in BRCA1-mutant cells. Nature 2017, 551, 590–595.
- Knijnenburg, T.A.; Wang, L.; Zimmermann, M.T.; Chambwe, N.; Gao, G.F.; Cherniack, A.D.; Fan, H.; Shen, H.; Way, G.P.; Greene, C.S.; et al. Genomic and Molecular Landscape of DNA Damage Repair Deficiency across The Cancer Genome Atlas. Cell Rep. 2018, 23, 239–254.e6.
- Davoli, T.; Uno, H.; Wooten, E.C.; Elledge, S.J. Tumor aneuploidy correlates with markers of immune evasion and with reduced response to immunotherapy. Science 2017, 355, eaaf8399.
- Kim, H.S.; Cha, H.; Kim, J.; Park, W.Y.; La Choi, Y.; Sun, J.M.; Ahn, J.S.; Ahn, M.J.; Park, K.; Lee, S.H. Genomic scoring to determine clinical benefit of immunotherapy by targeted sequencing. Eur. J. Cancer 2019, 120, 65–74.
- Piro, G.; Carbone, C.; Carbognin, L.; Pilotto, S.; Ciccarese, C.; Iacovelli, R.; Milella, M.; Bria, E.; Tortora, G. Revising PTEN in the Era of Immunotherapy: New Perspectives for an Old Story. Cancers 2019, 11, 1525.
- Casey, S.C.; Baylot, V.; Felsher, D.W. MYC: Master Regulator of Immune Privilege. Trends Immunol. 2017, 38, 298–305.
- McGranahan, N.; Rosenthal, R.; Hiley, C.T.; Rowan, A.J.; Watkins, T.B.K.; Wilson, G.A.; Birkbak, N.J.; Veeriah, S.; Van Loo, P.; Herrero, J.; et al. Allele-Specific HLA Loss and Immune Escape in Lung Cancer Evolution. Cell 2017, 171, 1259–1271.e11.
- Corrales, L.; Gajewski, T.F. Molecular pathways: Targeting the Stimulator of Interferon Genes (STING) in the immunotherapy of cancer. Clin. Cancer Res. 2015, 21, 4774–4779.
- Pantelidou, C.; Sonzogni, O.; Taveira, M.D.O.; Mehta, A.K.; Kothari, A.; Wang, D.; Visal, T.; Li, M.K.; Pinto, J.; Castrillon, J.A.; et al. Parp inhibitor efficacy depends on CD8+ T-cell recruitment via intratumoral sting pathway activation in brca-deficient models of triple-negative breast cancer. Cancer Discov. 2019, 9, 722–737.
- Zitvogel, L.; Galluzzi, L.; Kepp, O.; Smyth, M.J.; Kroemer, G. Type I interferons in anticancer immunity. Nat. Rev. Immunol. 2015, 15, 405–414.
- Corrales, L.; Glickman, L.H.; McWhirter, S.M.; Kanne, D.B.; Sivick, K.E.; Katibah, G.E.; Woo, S.R.; Lemmens, E.; Banda, T.; Leong, J.J.; et al. Direct Activation of STING in the Tumor Microenvironment Leads to Potent and Systemic Tumor Regression and Immunity. Cell Rep. 2015, 11, 1018–1030.
- Ramanjulu, J.M.; Pesiridis, G.S.; Yang, J.; Concha, N.; Singhaus, R.; Zhang, S.Y.; Tran, J.L.; Moore, P.; Lehmann, S.; Eberl, H.C.; et al. Design of amidobenzimidazole STING receptor agonists with systemic activity. Nature 2018, 564, 439–443.
- Härtlova, A.; Erttmann, S.F.; Raffi, F.A.M.; Schmalz, A.M.; Resch, U.; Anugula, S.; Lienenklaus, S.; Nilsson, L.M.; Kröger, A.; Nilsson, J.A.; et al. DNA Damage Primes the Type I Interferon System via the Cytosolic DNA Sensor STING to Promote Anti-Microbial Innate Immunity. Immunity 2015, 42, 332–343.
- Parkes, E.E.; Walker, S.M.; Taggart, L.E.; McCabe, N.; Knight, L.A.; Wilkinson, R.; McCloskey, K.D.; Buckley, N.E.; Savage, K.I.; Salto-Tellez, M.; et al. Activation of STING-dependent innate immune signaling by s-phase-specific DNA damage in breast cancer. J. Natl. Cancer Inst. 2017, 109, djw199.
- Chen, D.S.; Mellman, I. Elements of cancer immunity and the cancer-immune set point. Nature 2017, 541, 321–330.
- Green, A.R.; Aleskandarany, M.A.; Ali, R.; Hodgson, E.G.; Atabani, S.; De Souza, K.; Rakha, E.A.; Ellis, I.O.; Madhusudan, S. Clinical impact of tumor DNA repair expression and T-cell infiltration in breast cancers. Cancer Immunol. Res. 2017, 5, 292–299.
- Loibl, S.; Weber, K.E.; Timms, K.M.; Elkin, E.P.; Hahnen, E.; Fasching, P.A.; Lederer, B.; Denkert, C.; Schneeweiss, A.; Braun, S.; et al. Survival analysis of carboplatin added to an anthracycline/taxane-based neoadjuvant chemotherapy and HRD score as predictor of response—final results from GeparSixto. Ann. Oncol. 2018, 29, 2341–2347.
- Solinas, C.; Marcoux, D.; Garaud, S.; Vitória, J.R.; Van den Eynden, G.; de Wind, A.; De Silva, P.; Boisson, A.; Craciun, L.; Larsimont, D.; et al. BRCA gene mutations do not shape the extent and organization of tumor infiltrating lymphocytes in triple negative breast cancer. Cancer Lett. 2019, 450, 88–97.
- Sobral-Leite, M.; Van De Vijver, K.; Michaut, M.; Van Der Linden, R.; Hooijer, G.K.J.; Horlings, H.M.; Severson, T.M.; Mulligan, A.M.; Weerasooriya, N.; Sanders, J.; et al. Assessment of PD-L1 expression across breast cancer molecular subtypes, in relation to mutation rate, BRCA1-like status, tumor-infiltrating immune cells and survival. Oncoimmunology 2018, 7, e1509820.
- Samstein, R.M.; Krishna, C.; Ma, X.; Pei, X.; Lee, K.-W.; Makarov, V.; Kuo, F.; Chung, J.; Srivastava, R.M.; Purohit, T.A.; et al. Mutations in BRCA1 and BRCA2 differentially affect the tumor microenvironment and response to checkpoint blockade immunotherapy. Nat. Cancer 2020, 1, 1188–1203.
- Strickland, K.C.; Howitt, B.E.; Shukla, S.A.; Rodig, S.; Ritterhouse, L.L.; Liu, J.F.; Garber, J.E.; Chowdhury, D.; Wu, C.J.; D’Andrea, A.D.; et al. Association and prognostic significance of BRCA1/2-mutation status with neoantigen load, number of tumor-infiltrating lymphocytes and expression of PD-1/PD-L1 in high grade serous ovarian cancer. Oncotarget 2016, 7, 13587–13598.
- Clarke, B.; Tinker, A.V.; Lee, C.H.; Subramanian, S.; Van De Rijn, M.; Turbin, D.; Kalloger, S.; Han, G.; Ceballos, K.; Cadungog, M.G.; et al. Intraepithelial T cells and prognosis in ovarian carcinoma: Novel associations with stage, tumor type, and BRCA1 loss. Mod. Pathol. 2009, 22, 393–402.
- Wieser, V.; Gaugg, I.; Fleischer, M.; Shivalingaiah, G.; Wenzel, S.; Sprung, S.; Lax, S.F.; Zeimet, A.G.; Fiegl, H.; Marth, C. BRCA1/2 and TP53 mutation status associates with PD-1 and PD-L1 expression in ovarian cancer. Oncotarget 2018, 9, 17501–17511.
- McAlpine, J.N.; Porter, H.; Köbel, M.; Nelson, B.H.; Prentice, L.M.; Kalloger, S.E.; Senz, J.; Milne, K.; Ding, J.; Shah, S.P.; et al. BRCA1 and BRCA2 mutations correlate with TP53 abnormalities and presence of immune cell infiltrates in ovarian high-grade serous carcinoma. Mod. Pathol. 2012, 25, 740–750.
- Whitehair, R.; Peres, L.C.; Mills, A.M. Expression of the Immune Checkpoints LAG-3 and PD-L1 in High-grade Serous Ovarian Carcinoma. Int. J. Gynecol. Pathol. 2019, 39, 558–566.
- Dai, Y.; Sun, C.; Feng, Y.; Jia, Q.; Zhu, B. Potent immunogenicity in BRCA1-mutated patients with high-grade serous ovarian carcinoma. J. Cell. Mol. Med. 2018, 22, 3979–3986.
- Jenzer, M.; Keß, P.; Nientiedt, C.; Endris, V.; Kippenberger, M.; Leichsenring, J.; Stögbauer, F.; Haimes, J.; Mishkin, S.; Kudlow, B.; et al. The BRCA2 mutation status shapes the immune phenotype of prostate cancer. Cancer Immunol. Immunother. 2019, 68, 1621–1633.
- Tumeh, P.C.; Harview, C.L.; Yearley, J.H.; Shintaku, I.P.; Taylor, E.J.M.; Robert, L.; Chmielowski, B.; Spasic, M.; Henry, G.; Ciobanu, V.; et al. PD-1 blockade induces responses by inhibiting adaptive immune resistance. Nature 2014, 515, 568–571.
- Rosenberg, J.E.; Hoffman-Censits, J.; Powles, T.; van der Heijden, M.S.; Balar, A.V.; Necchi, A.; Dawson, N.; O’Donnell, P.H.; Balmanoukian, A.; Loriot, Y.; et al. Atezolizumab in patients with locally advanced and metastatic urothelial carcinoma who have progressed following treatment with platinum-based chemotherapy: A single-arm, multicentre, phase 2 trial. Lancet 2016, 387, 1909–1920.
- Helmink, B.A.; Reddy, S.M.; Gao, J.; Zhang, S.; Basar, R.; Thakur, R.; Yizhak, K.; Sade-Feldman, M.; Blando, J.; Han, G.; et al. B cells and tertiary lymphoid structures promote immunotherapy response. Nature 2020, 577, 549–555.
- Aguiar, P.N.; De Mello, R.A.; Hall, P.; Tadokoro, H.; De Lima Lopes, G. PD-L1 expression as a predictive biomarker in advanced non-small-cell lung cancer: Updated survival data. Immunotherapy 2017, 9, 499–506.
- Telli, M.L.; Timms, K.M.; Reid, J.; Hennessy, B.; Mills, G.B.; Jensen, K.C.; Szallasi, Z.; Barry, W.T.; Winer, E.P.; Tung, N.M.; et al. Homologous recombination deficiency (hrd) score predicts response to platinum-containing neoadjuvant chemotherapy in patients with triple-negative breast cancer. Clin. Cancer Res. 2016, 22, 3764–3773.
- Chen, H.; Wu, J.; Zhang, Z.; Tang, Y.; Li, X.; Liu, S.; Cao, S.; Li, X. Association between BRCA status and triple-negative breast cancer: A meta-analysis. Front. Pharmacol. 2018, 9, 909.
- Swoboda, A.; Nanda, R. Immune Checkpoint Blockade for Breast Cancer. In Cancer Treatment and Research; Springer International Publishing: New York, NY, USA, 2018; Volume 173, pp. 155–165.
- Schmid, P.; Adams, S.; Rugo, H.S.; Schneeweiss, A.; Barrios, C.H.; Iwata, H.; Diéras, V.; Hegg, R.; Im, S.-A.; Shaw Wright, G.; et al. Atezolizumab and Nab-Paclitaxel in Advanced Triple-Negative Breast Cancer. N. Engl. J. Med. 2018, 379, 2108–2121.
- Emens, L.A.; Molinero, L.; Loi, S.; Rugo, H.S.; Schneeweiss, A.; Diéras, V.; Iwata, H.; Barrios, C.H.; Nechaeva, M.; Nguyen Duc, A.; et al. Atezolizumab and nab-Paclitaxel in Advanced Triple-Negative Breast Cancer: Biomarker Evaluation of the IMpassion130 Study (CHB); Arkhangelsk Regional Clinical. J. Nat. Cancer Inst. 2021, djab004.
- Voorwerk, L.; Slagter, M.; Horlings, H.M.; Sikorska, K.; van de Vijver, K.K.; de Maaker, M.; Nederlof, I.; Kluin, R.J.C.; Warren, S.; Ong, S.F.; et al. Immune induction strategies in metastatic triple-negative breast cancer to enhance the sensitivity to PD-1 blockade: The TONIC trial. Nat. Med. 2019, 25, 920–928.
- Matulonis, U.A.; Shapira-Frommer, R.; Santin, A.D.; Lisyanskaya, A.S.; Pignata, S.; Vergote, I.; Raspagliesi, F.; Sonke, G.S.; Birrer, M.; Provencher, D.M.; et al. Antitumor activity and safety of pembrolizumab in patients with advanced recurrent ovarian cancer: Results from the phase II KEYNOTE-100 study. Ann. Oncol. 2019, 30, 1080–1087.
- Disis, M.L.; Taylor, M.H.; Kelly, K.; Beck, J.T.; Gordon, M.; Moore, K.M.; Patel, M.R.; Chaves, J.; Park, H.; Mita, A.C.; et al. Efficacy and Safety of Avelumab for Patients with Recurrent or Refractory Ovarian Cancer: Phase 1 b Results from the JAVELIN Solid Tumor Trial. JAMA Oncol. 2019, 5, 393–401.
- Matsuo, K.; Spragg, S.E.; Ciccone, M.A.; Blake, E.A.; Ricker, C.; Pham, H.Q.; Roman, L.D. Nivolumab use for BRCA gene mutation carriers with recurrent epithelial ovarian cancer: A case series. Gynecol. Oncol. Rep. 2018, 25, 98–101.
- Antonarakis, E.S.; Piulats, J.M.; Gross-Goupil, M.; Goh, J.; Ojamaa, K.; Hoimes, C.J.; Vaishampayan, U.; Berger, R.; Sezer, A.; Alanko, T.; et al. Pembrolizumab for treatment-refractory metastatic castration-resistant prostate cancer: Multicohort, open-label phase II KEYNOTE-199 study. J. Clin. Oncol. 2020, 38, 395–405.
- Sharma, P.; Pachynski, R.K.; Narayan, V.; Flechon, A.; Gravis, G.; Galsky, M.D.; Mahammedi, H.; Patnaik, A.; Subudhi, S.K.; Ciprotti, M.; et al. Initial results from a phase II study of nivolumab (NIVO) plus ipilimumab (IPI) for the treatment of metastatic castration-resistant prostate cancer (mCRPC.; CheckMate 650). J. Clin. Oncol. 2019, 37, 142.
- Sharma, P.; Pachynski, R.K.; Narayan, V.; Fléchon, A.; Gravis, G.; Galsky, M.D.; Mahammedi, H.; Patnaik, A.; Subudhi, S.K.; Ciprotti, M.; et al. Nivolumab Plus Ipilimumab for Metastatic Castration-Resistant Prostate Cancer: Preliminary Analysis of Patients in the CheckMate 650 Trial. Cancer Cell 2020, 38, 489–499.e3.
- Fradet, Y.; Bellmunt, J.; Vaughn, D.J.; Lee, J.L.; Fong, L.; Vogelzang, N.J.; Climent, M.A.; Petrylak, D.P.; Choueiri, T.K.; Necchi, A.; et al. Randomized phase III KEYNOTE-045 trial of pembrolizumab versus paclitaxel, docetaxel, or vinflunine in recurrent advanced urothelial cancer: Results of >2 years of follow-up. Ann. Oncol. 2019, 30, 970–976.
- Alva, A.; Csőszi, T.; Ozguroglu, M.; Matsubara, N.; Geczi, L.; Cheng, S.Y.; Fradet, Y.; Oudard, S.; Vulsteke, C.; Morales Barrera, R.; et al. LBA23—Pembrolizumab (p) combined with chemotherapy (C) vs C alone as first-line (1 L) therapy for advanced urothelial carcinoma (UC): KEYNOTE-361. Ann. Oncol. 2020, 31 (Suppl. S4), S1142–S1215.
- Powles, T.; van der Heijden, M.S.; Castellano, D.; Galsky, M.D.; Loriot, Y.; Petrylak, D.P.; Ogawa, O.; Park, S.H.; Lee, J.L.; De Giorgi, U.; et al. Durvalumab alone and durvalumab plus tremelimumab versus chemotherapy in previously untreated patients with unresectable, locally advanced or metastatic urothelial carcinoma (DANUBE): A randomised, open-label, multicentre, phase 3 trial. Lancet Oncol. 2020, 21, 1574–1588.
- Teo, M.Y.; Seier, K.; Ostrovnaya, I.; Regazzi, A.M.; Kania, B.E.; Moran, M.M.; Cipolla, C.K.; Bluth, M.J.; Chaim, J.; Al-Ahmadie, H.; et al. Alterations in DNA damage response and repair genes as potential marker of clinical benefit from PD-1/PD-L1 blockade in advanced urothelial cancers. J. Clin. Oncol. 2018, 36, 1685–1694.
- Hsiehchen, D.; Hsieh, A.; Samstein, R.M.; Wang, T.; Morris, L.G.T.; Correspondence, H.Z.; Lu, T.; Beg, M.S.; Gerber, D.E.; Zhu, H. DNA Repair Gene Mutations as Predictors of Immune Checkpoint Inhibitor Response beyond Tumor Mutation Burden ll. Cell Rep Med. 2020, 1, 100034.
- Liu, J.F.; Gordon, M.; Veneris, J.; Braiteh, F.; Balmanoukian, A.; Eder, J.P.; Oaknin, A.; Hamilton, E.; Wang, Y.; Sarkar, I.; et al. Safety, clinical activity and biomarker assessments of atezolizumab from a Phase I study in advanced/recurrent ovarian and uterine cancers. Gynecol. Oncol. 2019, 154, 314–322.
- Marabelle, A.; Le, D.T.; Ascierto, P.A.; Di Giacomo, A.M.; de Jesus-Acosta, A.; Delord, J.P.; Geva, R.; Gottfried, M.; Penel, N.; Hansen, A.R.; et al. Efficacy of pembrolizumab in patients with noncolorectal high microsatellite instability/ mismatch repair–deficient cancer: Results from the phase II KEYNOTE-158 study. J. Clin. Oncol. 2020, 38, 1–10.
- Boudadi, K.; Suzman, D.L.; Anagnostou, V.; Fu, W.; Luber, B.; Wang, H.; Niknafs, N.; White, J.R.; Silberstein, J.L.; Sullivan, R.; et al. Ipilimumab plus nivolumab and DNA-repair defects in AR-V7-expressing metastatic prostate cancer. Oncotarget 2018, 9, 28561–28571.
- Song, P.; Yang, D.; Wang, H.; Cui, X.; Si, X.; Zhang, X.; Zhang, L. Relationship between the efficacy of immunotherapy and characteristics of specific tumor mutation genes in non-small cell lung cancer patients. Thorac. Cancer 2020, 11, 1647–1654.
- Labriola, M.K.; Zhu, J.; Gupta, R.; McCall, S.; Jackson, J.; Kong, E.F.; White, J.R.; Cerqueira, G.; Gerding, K.; Simmons, J.K.; et al. Characterization of tumor mutation burden, PD-L1 and DNA repair genes to assess relationship to immune checkpoint inhibitors response in metastatic renal cell carcinoma. J. Immunother. Cancer 2020, 8, 319.
- Hugo, W.; Zaretsky, J.M.; Sun, L.; Song, C.; Moreno, B.H.; Hu-Lieskovan, S.; Berent-Maoz, B.; Pang, J.; Chmielowski, B.; Cherry, G.; et al. Genomic and Transcriptomic Features of Response to Anti-PD-1 Therapy in Metastatic Melanoma. Cell 2016, 165, 35–44.
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