Urine Metabolomics and Exercise in ME/CFS
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  • Release Date: 2023-04-10
  • ME/CFS
  • metabolomics
  • urine
  • exercise
Video Introduction

This video is adapted from 10.3390/ijms24043685

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a debilitating disease with unknown etiology or effective treatments. Post-exertional malaise (PEM) is a key symptom that distinguishes ME/CFS patients. Investigating changes in the urine metabolome between ME/CFS patients and healthy subjects following exertion may help us understand PEM. The aim of this pilot study was to comprehensively characterize the urine metabolomes of eight female healthy sedentary control subjects and ten female ME/CFS patients in response to a maximal cardiopulmonary exercise test (CPET). Each subject provided urine samples at baseline and 24 h post-exercise. A total of 1403 metabolites were detected via LC-MS/MS by Metabolon® including amino acids, carbohydrates, lipids, nucleotides, cofactors and vitamins, xenobiotics, and unknown compounds. Using a linear mixed effects model, pathway enrichment analysis, topology analysis, and correlations between urine and plasma metabolite levels, significant differences were discovered between controls and ME/CFS patients in many lipid (steroids, acyl carnitines and acyl glycines) and amino acid subpathways (cysteine, methionine, SAM, and taurine; leucine, isoleucine, and valine; polyamine; tryptophan; and urea cycle, arginine and proline). The most unanticipated discovery is the lack of changes in the urine metabolome of ME/CFS patients during recovery while significant changes are induced in controls after CPET, potentially demonstrating the lack of adaptation to a severe stress in ME/CFS patients.

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Hanson, M.; Glass, K.A.; Germain, A.; Huang, Y.V. Urine Metabolomics and Exercise in ME/CFS. Encyclopedia. Available online: https://encyclopedia.pub/video/video_detail/701 (accessed on 15 April 2024).
Hanson M, Glass KA, Germain A, Huang YV. Urine Metabolomics and Exercise in ME/CFS. Encyclopedia. Available at: https://encyclopedia.pub/video/video_detail/701. Accessed April 15, 2024.
Hanson, Maureen, Katherine A. Glass, Arnaud Germain, Yuhsin V. Huang. "Urine Metabolomics and Exercise in ME/CFS" Encyclopedia, https://encyclopedia.pub/video/video_detail/701 (accessed April 15, 2024).
Hanson, M., Glass, K.A., Germain, A., & Huang, Y.V. (2023, April 10). Urine Metabolomics and Exercise in ME/CFS. In Encyclopedia. https://encyclopedia.pub/video/video_detail/701
Hanson, Maureen, et al. "Urine Metabolomics and Exercise in ME/CFS." Encyclopedia. Web. 10 April, 2023.