HOCl and Reactive Halogenated Species
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  • Release Date: 2022-10-21
  • hypochlorous acid
  • myeloperoxidase
  • neutrophils
  • fluorescent probes
Video Introduction

This video is adapted from 10.3390/antiox11091719

Hypochlorous acid (HOCl) derived from hydrogen peroxide and chloride anion by myeloperoxidase (MPO) plays a significant role in physiological and pathological processes. Researchers report a phenoxazine-based fluorescent probe Celestine Blue B (CB) that is applicable for HOCl detection in living cells and for assaying the chlorinating activity of MPO. A remarkable selectivity and sensitivity (limit of detection is 32 nM), along with a rapid “turn-on” response of CB to HOCl was demonstrated. Furthermore, the probe was able to detect endogenous HOCl and reactive halogenated species by fluorescence spectroscopy, confocal microscopy, and flow cytometry techniques. Hence, CB is a promising tool for investigating the role of HOCl in health and disease and for screening the drugs capable of regulating MPO activity. 

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Sokolov, A.V.;  Reut, V.E.;  Kozlov, S.O.;  Kudryavtsev, I.V.;  Grudinina, N.A.;  Kostevich, V.A.;  Gorbunov, N.P.;  Grigorieva, D.V.;  Kalvinkovskaya, J.A.;  Bushuk, S.B.; et al. HOCl and Reactive Halogenated Species. Encyclopedia. Available online: https://encyclopedia.pub/video/video_detail/449 (accessed on 25 April 2024).
Sokolov AV,  Reut VE,  Kozlov SO,  Kudryavtsev IV,  Grudinina NA,  Kostevich VA, et al. HOCl and Reactive Halogenated Species. Encyclopedia. Available at: https://encyclopedia.pub/video/video_detail/449. Accessed April 25, 2024.
Sokolov, Alexey V., Veronika E. Reut, Stanislav O. Kozlov, Igor V. Kudryavtsev, Natalia A. Grudinina, Valeria A. Kostevich, Nikolay P. Gorbunov, Daria V. Grigorieva, Julia A. Kalvinkovskaya, Sergey B. Bushuk, et al. "HOCl and Reactive Halogenated Species" Encyclopedia, https://encyclopedia.pub/video/video_detail/449 (accessed April 25, 2024).
Sokolov, A.V.,  Reut, V.E.,  Kozlov, S.O.,  Kudryavtsev, I.V.,  Grudinina, N.A.,  Kostevich, V.A.,  Gorbunov, N.P.,  Grigorieva, D.V.,  Kalvinkovskaya, J.A.,  Bushuk, S.B.,  Varfolomeeva, E.Y.,  Fedorova, N.D.,  Gorudko, I.V., Panasenko, O., & Vasilyev, V.B. (2022, October 21). HOCl and Reactive Halogenated Species. In Encyclopedia. https://encyclopedia.pub/video/video_detail/449
Sokolov, Alexey V., et al. "HOCl and Reactive Halogenated Species." Encyclopedia. Web. 21 October, 2022.