Honey bees collect a multitude of substances from plants, including nectar, pollen, and a lesser-known resinous substance called propolis. Honey bees line their colonies with propolis to fill in cracks and potentially aid in their defense against pathogens such as fungi, bacteria, and viruses. Different plants contain different types of chemicals that are collected by bees to form propolis, and so one would expect the plants that bees visit to influence the quality of the propolis contained within honey bee colonies. This video explored the chemical composition and antibacterial effects of propolis collected from apiaries that were surrounded by different types of land use patterns in Iowa, USA. Propolis samples collected from colonies that were surrounded by the highest levels of agriculture had the lowest abundance of chemical compounds and also the lowest antimicrobial activity detected for two of the bacteria species studied. These results add to a growing body of work that suggests that high intensity agricultural land use negatively impacts multiple aspects of honey bee colony health.