Cholinergic-like Neurons
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  • Release Date: 2022-01-20
  • mesenchymal stem cells
  • umbilical cord
  • blood
  • membrane
  • biopolymer
Video Introduction

This video is adapted from 10.3390/membranes11080598

This video aimed to differentiate human mesenchymal stem cells (hMSCs) from the human umbilical cord in cholinergic-like neurons using a natural membrane. The isolation of hMSCs from Wharton’s jelly (WJ) was carried out using “explant” and mononuclear cells by the density gradient from umbilical blood and characterized by flow cytometry. hMSCs were seeded in a natural functional biopolymer membrane to produce neurospheres. RT-PCR was performed on hMSCs and neurospheres derived from the umbilical cord. Neural precursor cells were subjected to a standard cholinergic-like neuron differentiation protocol. Dissociated neurospheres, neural precursor cells, and cholinergic-like neurons were characterized by immunocytochemistry. 

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Athayde Teixeira De Carvalho, K.; Stricker, P.; Souza, D.; Irioda, A.C.; Mogharbel, B.; Leite, J.R.; R. De Araújo, A.; Graeff, C.F.; Chachques, J.C. Cholinergic-like Neurons. Encyclopedia. Available online: (accessed on 21 April 2024).
Athayde Teixeira De Carvalho K, Stricker P, Souza D, Irioda AC, Mogharbel B, Leite JR, et al. Cholinergic-like Neurons. Encyclopedia. Available at: Accessed April 21, 2024.
Athayde Teixeira De Carvalho, Katherine, Priscila Stricker, Daiany Souza, Ana Carolina Irioda, Bassam Mogharbel, Jose Roberto Leite, Alyne R. De Araújo, Carlos Fo Graeff, Juan C. Chachques. "Cholinergic-like Neurons" Encyclopedia, (accessed April 21, 2024).
Athayde Teixeira De Carvalho, K., Stricker, P., Souza, D., Irioda, A.C., Mogharbel, B., Leite, J.R., R. De Araújo, A., Graeff, C.F., & Chachques, J.C. (2022, January 20). Cholinergic-like Neurons. In Encyclopedia.
Athayde Teixeira De Carvalho, Katherine, et al. "Cholinergic-like Neurons." Encyclopedia. Web. 20 January, 2022.