Bacteroides Species in Children with Type 1 Diabetes
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  • Release Date: 2022-01-18
  • type 1 diabetes
  • Phocaeicola dorei
  • Parabacteroides distasonis
  • Bacteroides uniformis
  • invasion
  • phage
Video Introduction

This video is adapted from 10.3390/microorganisms9071436

In the previous study the enrichment of the intestinal proteome of type 1 diabetes (T1D) children with Bacteroides proteins was observed, which led them to their current study that aimed to isolate and characterize Bacteroides species from fecal samples of T1D and control children. Repetitive sequence-based PCR (rep-PCR) was used for typing the isolated Bacteroides species. The antibiotic susceptibility and mucinolytic activity of the isolates was determined. The quantification of specific bacterial groups in the fecal samples was determined by qPCR. The ability to adhere and invade the human colonic cell line HT29-MTX-E12 of strains of P. dorei, B. uniformis and P. distasonis was determined and their whole genome sequencing was performed. The results showed similar numbers of Bacteroides species in T1D and control samples, but unique Bacteroides species and a higher recovery of P. distasonis from T1D samples was observed. Rep-PCR grouped the different Bacteroides species, but no discrimination by origin was achieved. T1D children showed a significant increase in Proteobacteria and a depletion in Lactobacillus sp. All tested P. dorei, B. uniformis and P. distasonis were able to adhere to HT29-MTX-E12 cells but significant differences (p < 0.05) in the ability to invade was observed. The highest ability to invade was exhibited by P. distasonis PtF D14MH1 and P. dorei PtFD16P1, while B. uniformis strains were unable to invade. The damage to tight junctions was also observed. The presence of Lactobacillus sp. inhibited the invasion ability of P. distasonis PtF D14MH1 but not P. dorei PtFD16P1. Sequences of agonist peptides of the human natural preproinsulin and the insulin B chain insB:9-23 peptide mimics were identified. The results reported in the study stresses the continued efforts required to clarify the link between T1D and gut microbiota.

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Faleiro, M.L.;  Calha, M.; Cardoso, Y. Bacteroides Species in Children with Type 1 Diabetes. Encyclopedia. Available online: (accessed on 22 April 2024).
Faleiro ML,  Calha M, Cardoso Y. Bacteroides Species in Children with Type 1 Diabetes. Encyclopedia. Available at: Accessed April 22, 2024.
Faleiro, Maria Leonor, Manuela Calha, Yameric Cardoso. "Bacteroides Species in Children with Type 1 Diabetes" Encyclopedia, (accessed April 22, 2024).
Faleiro, M.L.,  Calha, M., & Cardoso, Y. (2022, January 18). Bacteroides Species in Children with Type 1 Diabetes. In Encyclopedia.
Faleiro, Maria Leonor, et al. "Bacteroides Species in Children with Type 1 Diabetes." Encyclopedia. Web. 18 January, 2022.