Spheroids Cytotoxic Response to an Anticancer Peptide
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  • Release Date: 21 Dec 2021
  • 3D cell culture
  • anticancer peptides
  • breast cancer
  • cell monolayers
Video Introduction

This video is adapted from 10.3390/pharmaceutics13111863

Breast cancer (BC) is the most commonly diagnosed cancer in women and one of the most common causes of cancer-related deaths. Despite intense research efforts, BC treatment still remains challenging. Improved drug development strategies are needed for impactful benefit to patients. Current preclinical studies rely mostly on cell-based screenings, using two-dimensional (2D) cell monolayers that do not mimic in vivo tumors properly. Researchers explored the development and characterization of three-dimensional (3D) models, named spheroids, of the most aggressive BC subtypes (triple-negative breast cancer-TNBC; and human-epidermal growth receptor-2-HER2+), using the liquid overlay technique with several selected cell lines. In these cell line-derived spheroids, authors studied cell density, proliferation, ultrastructure, apoptosis, reactive oxygen species (ROS) production, and cell permeabilization (live/dead). The results showed a formation of compact and homogeneous spheroids on day 7 after seeding 2000 cells/well for MDA-MB-231 and 5000 cells/well for BT-20 and BT-474. Next, they compared the efficacy of a model anticancer peptide (ACP) in cell monolayers and spheroids. Overall, the results demonstrated spheroids to be less sensitive to treatment than cell monolayers, revealing the need for more robust models in drug development.

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Neves, V.; Cavaco, M.; Andreu, D.; Castanho, M. Spheroids Cytotoxic Response to an Anticancer Peptide. Encyclopedia. Available online: https://encyclopedia.pub/video/video_detail/150 (accessed on 29 February 2024).
Neves V, Cavaco M, Andreu D, Castanho M. Spheroids Cytotoxic Response to an Anticancer Peptide. Encyclopedia. Available at: https://encyclopedia.pub/video/video_detail/150. Accessed February 29, 2024.
Neves, Vera, Marco Cavaco, David Andreu, Miguel Castanho. "Spheroids Cytotoxic Response to an Anticancer Peptide" Encyclopedia, https://encyclopedia.pub/video/video_detail/150 (accessed February 29, 2024).
Neves, V., Cavaco, M., Andreu, D., & Castanho, M. (2021, December 21). Spheroids Cytotoxic Response to an Anticancer Peptide. In Encyclopedia. https://encyclopedia.pub/video/video_detail/150
Neves, Vera, et al. "Spheroids Cytotoxic Response to an Anticancer Peptide." Encyclopedia. Web. 21 December, 2021.