The antimicrobial properties of CNTs mostly depend on their composition, surface modification, specific microorganisms, and surrounding environment. Most of the possible antimicrobial mechanisms of CNTs are based on the invasion of the microorganism cell wall and the induction of structural damage. Oxidative stress induction via the production of toxic materials and reactive oxygen species (ROS) in which electrons are removed from the microbial surface and cell death would occur [11]. Some researchers confirm that when CNT size decreases, their surface-to-volume ratio increases and ends in stronger interaction with the microorganism cell membrane. They explain that disruption of the cell membrane, metabolic procedure, and morphology, as well as the enhanced efflux of plasmid DNA, RNA, and cytoplasmic materials, are the main mechanisms of action of CNTs’ bacteriostatic properties [11,12,13,14,15,16,17,18]. The use of CNTs as novel drug-delivery systems for antibiotics will also increase their bioavailability and facilitate targeted therapy. Kang et al. first announced the size-dependent antibacterial properties of SWNT against E. coli in 2007. Their complementary studies declare that SWCNTs (single-wall carbon nanotubes) are more toxic to microorganisms and Gram-negative and Gram-positive bacteria in comparison to MWCNTs (multi-wall carbon nanotubes) [11,19,20,21,22]. Better penetration into the cell wall would occur for CNTs with a smaller diameter.

Chen et al., Rodrigues et al., and Liu et al. reported that the antibacterial activity of CNTs may also depend on microorganism properties including type and morphology, the mechanical properties of cell surfaces, and the growth state [12,27,28,29]. Chen et al. declare the hypothesis of ‘‘nano-darts’’ as the main cause of bacteria death [27]. Gram-positive bacteria including Staphylococcus aureus and Bacillus subtilis are more susceptible to single-wall carbon nanotubes due to their spherical shape and membrane softness [15,26]. Biofilms, free-floating, and rod-shaped cells are more resistant to the bactericidal activities of CNTs [29]. CNTs are chemically stable cargoes for the delivery of therapeutic molecules including antibiotics and antimicrobials. CNTs loaded with antibiotics would be a promising strategy to combat antibacterial resistance Moreover, due to their intrinsic antimicrobial activity, the emergence of drug-resistant strains has added to the significance of studies being undertaken on carbon nanotubes. CNT’s’ exclusive features in improving the efficiency of drugs such as antibiotics, reducing drug dosage, and antibiotic resistance have been studied in the destruction of Acinetobacter bumanii and the obtained results proved to be satisfactory [30].

In addition, MWCNT nanofluids as compounds with prevalent antibiotics such as Kanamycin and Streptomycin are effective in displaying superior characteristics including increased penetration into the bacterial membrane, heightened efficiency in lower concentrations compared to prevalent treatment dosages, and lower bacterial resistance to antibiotics in the treatment of M. fortuitum [31].

Treatment of the resistant strain of Klebsiella pneumoniae using the +f-MCWNTs antibiotic is sufficient for proving the antibiotic efficiency in lower dosages, reducing antibiotic resistance, and increasing the permeability of the cell wall toward the antibiotic due to the presence of MWCNTs [32].

They would be new options for the production of medical devices and prosthetic implants [12,33,34,35,36,37]. Malek et al. showed that silicone materials decorated with aligned multi-wall carbon nanotubes can reduce the possibility of biofilm formation by up to 60% and might be suggested as new material for medical device manufacturing [38]. Vagos et al. also demonstrated that a polydimethylsiloxane (PDMS) matrix containing 10% pure MWNTs was effective in the 20% reduction of E. coli adherence in simulated conditions to the urinary tract and offered this material for urinary tract medical devices [39].

Despite the promising potential of CNTs in biomedicine, the hydrophobic structure and innate toxicity of pristine and pure single-wall and multi-wall carbon nanotubes might be a drawback. However, surface functionalization would increase interaction with the cellular membrane and antimicrobial activity of CNTs. This would normally happen as a result of better aqueous dispersion, improved biocompatibility, and reduced toxicity for human cells [12]. It seems that functionalized MWCNTs with amine, carboxyl, nitrogen ions, and ethanolamine show good antibacterial properties against E. coli and S. aureus when used in medical devices [40,41,42,43]. Amine-Functionalized MWCNTs were reported to significantly increase E. coli and S. aureus MIC in comparison to PCL (poly ε-caprolactone) [41]. MWCNTs being functionalized using acylation reactions, supplemented by the use of INH medicine to obtain a proper dosage of the nano-medicine and heighten the efficiency and lower antibiotic resistance, have been proven effective in the treatment of Tuberculosis [44].

Similar results were reported by Zardini et al. [42]. Another study was conducted to investigate the level of pro-inflammatory cytokines in macrophages derived from THP1 and A549 cell strains contaminated by Klebsiella pneumoniae as the resistant strain, which was treated by f-MWCNTs+cip.

Numerous challenges have been reported in counteracting nosocomial infections. Carbon nanotubes, as functionalized nanofluids, are considered an appropriate approach for the treatment of this category of infections. The simultaneous prescription of functionalized carbon nanotubes as well as meropenem, in a nanofluid environment, was significantly effective in reducing the growth of the Pseudomonas aeruginosa strain. Moreover, through heightening drug stability, the carbon nanotubes were effective in reducing Pseudomonas aeruginosa antibiotic resistance in lower dilutions compared to antibiotics [46].

The effectiveness of MWCNT nanofluids, being functionalized with a carboxylic acid, is vastly different from the effectiveness of non-functionalized multi-walled carbon nanotubes. It seems that this function has been modified after bacteria’s exposure to the nanofluid and, possibly, the lower bacterial growth rate can be attributed to the connection between functionalized MWCNT nanofluids and the bacterial membrane. This has resulted in the destruction of the membrane’s integrity and heightened antibiotic efficiency. Thus, functionalized MWCNTs would have antimicrobial impacts on Staphylococcus aureus and would overcome the antibiotic resistance of this strain [47].

In another study, different modes of drugs were examined as free-standing medicine, as functionalized MWCNTs, non-functionalized MWCNTs, a drug in combination with a non-functionalized MWCNT nanofluid, and as a drug in combination with a functionalized MWCNT nanofluid on the Klebsiella pneumoniae strain. The results suggest that the drug in combination with the functionalized MWCNT nanofluid was highly effective in inhibiting bacterial growth [48].

Highlighting the antimicrobial activity of amine-MWCNTs was associated with stronger interaction between the cationic nature of amine-MWCNT and negatively charged cells of bacteria and extensive cell membrane lysis, which led to its bactericidal effect. These outcomes suggested functionalized carbon nanotubes as novel nano-antimicrobial materials for the construction of medical devices and implants [12].

Carbon nanotubes are proposed as promising materials in the battle against antimicrobial drug resistance (AMR). MWCNT nanofluid conjugated with Isoniazid and Fluoxetine with a nano-drug delivery system is highly effective in treating infections as well as reducing drug resistance in Mycobacterium Tuberculosis clinical strains [49]. Covalent conjugation of cephalexin with PEGylated MWCNTs improved the bactericidal activity of cephalexin against Gram-negative and Gram-positive bacteria simply by their anti-adhesive characteristic [50]. Azithromycin conjugated with SWCNTs was also reported to show higher antibacterial activity against Micrococcus luteus [51]. Titanium discs coated with Rifampicin-MWCNTs showed better inhibition for the formation of a Staphylococcus epidermis biofilm [52].

Antimicrobial photodynamic therapy (APT) is a non-antibiotic agent for bacterial contamination. This idea was well supported by research performed on an NIR application along with carbon nanotubes conjugated with photosensitizers including porphyrin and DTTC (3,3′ –diethylthiatricarbocanine fluorophores) [53,54]. The results support the idea that APT would help kill Pseudomonas aeruginosa by increasing the temperature after laser irradiation [53,54].

CNTs conjugated with Antimicrobial peptides (AMP) including EP (epsilon-polylysine), PLL (polyelectrolytes poly (l-lysine), PGA (poly (L-glutamic acid), and Nisin were also investigated for application in medical devices. High antibacterial activities against E coli, P. aeruginosa, and Staphylococcus epidermis were also reported [55,56,57].

Cell lysis by lytic enzymes such as LSZ (lysozyme), Lysostaphin, and Laccase have been proposed as promising alternative antimicrobials specially designed to combat methicillin-resistant S. aureus, biofilm formation, and anti-sporicidal activity of B. cereus/B. anthracis [58,59,60].

The application of bio-nanofilms in medical devices and implants, which are generally silver or metal-coated CNT-based films, was found to be effective against a broad range of bacteria through reduced bacterial adhesion [12]. Among different Bio-nanofilms, AgNPs (silver nanoparticles), AgNPs-DNA (silver nanoparticles stabilized with DNA), ZnHa (zinc hydroxyapatite), and PdNPs (palladium nanoparticles) were the most evaluated CNT-metal conjugates for antibacterial activity against E. coli, A. aureus, B subtilis, P aeruginosa, S. epidermis, and K. pneumonia [61,62,63,64,65,66,67].

Polymers have been widely applied to develop CNT Nanocomposites with improved structural, mechanical, biocompatibility, biological stability, and cost effectiveness [12,28,68]. Among various polymeric CNT composites, PEG (polyethylene glycol), PLGA (poly(lactic-co-glycolic acid), PEI (Poly ethyleneimine), and polypyrrole composites were found to achieve significant inactivation of a broad spectrum of Gram-negative and Gram-positive bacteria, including E. coli and S. epidermis, up to 98% when used as a wound dressing or in medical devices [37,68,69,70,71].

Chitosan-MWCNT nanocomposites have been enormously explored as probable antimicrobial surfaces for wide implementation in biomedical applications including wound dressing, tissue engineering, biosensing, and drug delivery [72]. It has been stated that the incorporation of MWCNTs would be an added value that intensifies the innate antibacterial/antifungal activity of chitosan. Carboxymethyl chitosan (CMCS), aminohydrazide, and aminosalicyl-hydrazid-cross-linked chitosan are some examples of chemically modified chitosan exploited with functionalized MWCNTs to achieve stronger antimicrobial activity against Gram-positive bacteria [34,35,73]. Shi et al. and Pramanik et al. [70,74] studied the antimicrobial and anti-adhesive properties of functionalized-MWCNT/hyperbranched poly(ester amide) (HBPEA) thin films. The higher the loading of MWCNTs, the less cell adhesion was observed to this functionalized MWCNT-HBPEA, and specific Gram-positive antibacterial activity was achieved. PEG-functionalized CNTs and thermoplastic polyurethane (TPU)-PEG electro-spun Nanofibers were highlighted as promising CNT/polymer biomaterials with less possibility of auto aggregation [70]. Anti-adhesive properties of CNT/polymer Nanocomposites are partially related to the surface smoothness and uniformity of the polymer coating as well as π–π interactions [70,74,75,76,77,78].

Polymeric surfactants are another tool in the preparation of stable dispersed CNTs. Researchers declare that the antibacterial activity of single-walled carbon nanotubes (SWCNTs) is dispersed in surfactant solutions. Sodium cholate showed the weakest antibacterial activity against S. enterica, E. coli, and Enterococcus faecium in comparison to sodium dodecylbenzene sulfonate and sodium dodecyl sulfate. It was reported that increasing nanotube concentrations up to 1.5 mg/mL will potentiate the antibacterial activity of CNTs as an effective alternative to antibiotics, especially regarding multidrug-resistant bacterial strains [13].

It could be concluded that metals, antimicrobial agents, and polymers play key roles in the antibacterial and AMR properties of CNT-based compounds. The synergistic effect of CNT-based antimicrobials was suggested to be caused by the inhibition of the cell wall, inhibition of protein synthesis, increase in cell membrane permeability, loss of membrane integrity and potential, protein dysfunction, oxidative stress, promotion of microorganism cell wall contact, and change in surface hydrophobicity and roughness as a bacterial anti-adhesion strategy [12,55,56,57].

Graphene materials (GMs) are one of the new carbon-based, alternative strategies for using materials with inherent antibacterial properties to prevent infection. Recent research has suggested that the antibacterial activity of graphene and graphene-derived materials occurs after direct physical and chemical interactions between GMs and bacteria, which cause the lethal degradation of cellular components, mainly proteins, nucleic acids, and lipids. GMs tend to accumulate in membrane proteoglycans, leading to membrane damage. GMs interrupt the replication phase by interacting with the hydrogen groups of the RNA/DNA of bacteria. They can also indirectly determine bacterial death after entering the physiological environment by activating the inflammatory pathway caused by active species [14].

The analysis results suggest that the graphene sheet is capable of automatically penetrating the dimer protein. The penetration of graphene sheets into the protein–protein interface would destabilize the Protein–Protein Interaction (PPI) by disturbing the hydrophobic interactions resulting in the decomposition of the protein complex [79].

Graphene is a carbon layer of the graphite structure, composed of hybridized carbon atoms linked by longitudinal bonds tightly packed into a honeycomb lattice to form a two-dimensional crystal [80,81]. Graphene derivatives include graphene oxide (GO) and reduced graphene oxide (rGO), which are created by chemical modifications in graphene to improve its properties and can be used in various fields [82]. GO have oxygen functional groups including hydroxyl, carboxyl, carbonyl, and epoxy which are mostly obtained from an oxidized graphene molecule and graphite acid oxidation. GO is insoluble in organic solvents such as alcohol, and toluene due to its strong hydrophilic properties. Furthermore, it is remarkably effective in force harvesting and electronic applications [82,83]. The GO structure includes many functional groups, which are capable of covalently binding to biological molecules and growth factors to strengthen cellular proliferation and differentiation. It can be deciphered that hydrophilic surfaces such as GO would be easily proliferated; however, upon regulating the rGO’s (hydrophilic) oxygen level and the use of appropriate additives, an efficient material for TE and medical purposes would be developed [84].

rGO is obtained by chemical or thermal reduction of oxygen in functional groups in GO material. In addition, rGO has a wide surface area and strength, high reactivity, and biocompatibility [85,86]. The antimicrobial properties of GMs depend largely on their lateral size, the number of layers, particle shape, surface modifications, agglomeration, and dispersion. Lateral size is a determining factor in the antimicrobial effectiveness of GMs. Research has confirmed that the larger the GM lateral size, the stronger the absorption capacity attributed to the higher surface energies.

According to the conducted research, the number of graphene layers has a major influence on its antimicrobial activity. Increasing the number of layers of GMs could enhance thickness and diminish dispersion. Moreover, the number of layers of GMs could increase the tendency to aggregate, leading to less contact between GMs and microorganisms. Generally, the number of layers influences the surface features that induce the basal plane’s antimicrobial activity, which shows both the edges and surface of GMs are important factors in antimicrobial activity. An example of this is the study carried out by Mangadlao, which revealed that an increased number of GO sheets have a stronger antimicrobial effect against E. coli [87,88].

The particle shape considerably influences the antimicrobial activity of nanoparticles. Studies have shown that nanoparticle shapes are essential for their interaction with the lipid bilayer in a translocation process. Additionally, the easy permeation of graphene nanoparticles into the cell membrane owing to the low energy barrier of these sharp-corner protruded particles can be caused by antimicrobial activity [89,90]. Akhavan and Ghaderi et al. reported that the sharp edges of graphene oxide nanowalls (GONWs) and graphene oxide nanowalls (RGNWs) significantly reduced the rate of survival of both E. coli and Staphylococcus aureus (S. aureus) [91].

The interaction between GMs and other molecules, such as proteins, lipids, DNA/RNA, and other materials is crucial for antimicrobial activity. The tendency of intact graphene to agglomerate potentially reduces its contact with other particles [88]. Surface modifications of graphene through covalent and noncovalent modulation have been found to play an important role in preventing particle agglomeration and, as a result, affect their antimicrobial activities [87,92,93]. Recent research has suggested that rGO has stronger antimicrobial activity than GO against S. aureus and E. coli [94]. According to other reports, rGO can inhibit the proliferation of E. coli, while no cytotoxicity has been observed in the case of GO [95].

Research has suggested that the antimicrobial effect of GMs may be increased by the effect of covalent modulation with oxygen-containing groups. Oxygen groups can influence the GMs’ amphipathic and chelating effect of the generators, which subsequently alters their antimicrobial activities [96,97]. Consequently, GMs can affect the survival of microorganisms through adsorption interactions between GMs and molecules, ions, and other substances [14,88].

GMs, due to the high surface energies. are susceptible to agglomeration that modulates the edge and surface characteristics of the nanoparticles and changes their antimicrobial activities. In the case of CNTs, one of the main factors that drive their antimicrobial activity is the tendency to aggregate, which reduces the surface area and changes the shape of the nanomaterials [98]. The density of GMs weakens their dispersibility and absorption, which changes the efficiency of the blades and thus reduces their interaction with microorganisms. It has been reported that rGO is stronger than GO in bacterial inactivation. This is attributed to the entrapment of E. coli and its ability to gradually cover the bacteria during the formation of rGO beads in suspension [88,99].

Different experimental conditions should be considered when evaluating the antimicrobial activities of GMs. Experimental conditions such as the state of the material applied, the type of bacteria (aerobic and anaerobic), the medium applied (in vitro and in vivo), and the genus of microorganisms such as the shape (rod and round) and class (Gram-positive and -negative). Controlling the growth of microorganisms is very important because each microorganism has its own capacity in physicochemical conditions. It has been reported that the antimicrobial activity of the rough surface of graphene layers is stronger against P. aeruginosa than against S. aureus. This phenomenon was interpreted as an indicator of the antimicrobial effect degree and is highly dependent on the selected bacterial species [88,100].

Summarizing GMs’ advantages, conductivity, mechanical properties, antibacterial properties, detection, and water decontamination are worth mentioning. GMs possess wide-ranging antibacterial and antiviral applications. GMs could be used for industrial water treatment to delete ions, bacteria, and other contaminants [101,102,103,104,105]. GMs’ activity is not targeted toward specific receptors or pathways, so resistance could be developed by bacteria after long exposure, which is the disadvantage of GMs application [14].

CNTs toxicity to human cells is a major concern that should be addressed carefully while focusing on its antimicrobial properties. Various kinds of research confirm that diameter, length, residual catalyst, metal content, surface coating, electronic structure, and dispersibility affects CNTs toxicity in bacteria and human cells [18]. The results of animal research suggest that long-time exposure to CNT would result in permanent inflammation, lung cancer, fibrosis, and the destruction of genes within the lung. The presence of MWCNTs within the human body would result in the production of cytokines such as TNF- α and IL-1 β from the immune cells involved in the development of toxicity. Moreover, SWCNTs would result in acute effects including inflammation, granuloma synthesis, collagen deposition, fibrosis, and genotoxicity within human lungs; however, the use of novel methods such as functionalization would assist researchers in the development of nanotubes with higher length, width, and curvature values, though with lower toxicity [106].

CNTs that are prepared by Arc discharge, electrolysis, laser ablation, chemical vapor deposition (CVD), and sono chemical/hydrothermal methods are suitable as electrochemical biosensors and antimicrobials. The synthesis method and extra modifying additives are critical parameters in CNTs’ applications. For example, carbon-nanotube array-based microfluidic devices and Molybdenum disulfide-MWCNTs (MoS₂-MWCNTs) are CNT-based biosensors with improved selectivity due to the negatively charged carboxyl group on MWCNTs for virus identification and chloramphenicol/dopamine detection, respectively [18,107,108,109]. In another study, the impacts of Bromocriptine (BRC)-conjugated MWCNTs on lung cancer cells (i.e., A549 and QU-DB) and MRC5 have been studied using MTT and Flow Cytometry tests.

The beneficial aspect of CNTs might seem a revolutionary strategy against increasing microbial infections in clinics and hospitals caused by ignorant usage of antimicrobial agents.

CNTs’ antimicrobial potency has attracted attention and interest in the usage of CNTs as coatings or dressings in medical devices and hospital settings to prevent nosocomial infections [18,58,111,112,113,114]. Membrane damage, ROS activation, suppressed metabolic activity oxidative stress, extraction of phospholipids, and DNA/RNA release are considered the main mechanisms for insight into CNTs’ antibacterial activity [18].

Functionalized CNTs with strong oxidizing groups will significantly improve their aqueous dispersivity for biotechnological applications [18,115]. Aggregation and dispersivity properties might be considered. Short-length SWCNTs show higher bactericidal activity due to the higher self-aggregation possibility [18]. Smaller diameters cause more damage to the cell membrane through more cell–surface interactions. Meanwhile, the presence of amorphous carbon species as impurities and carboxyl groups on CNTs’ surface directly affects CNTs’ toxicity and antibacterial activity. Therefore, highly purified, short-length, small-diameter, functionalized CNTs could be considered unique selective bactericidal agents [27,116,117,118,119].

Carbon nanomaterials are nanostructures containing impurities based on the applied synthesis, preparation, and purification methods. Metallic, nanographitic, and amorphous carbon-based impurities are the commonly found impurities in CNTs. Pumera et al. impressively explained how such impurities are capable of dramatically influencing redox properties as one of the mechanisms involved in their antimicrobial activity [120].

Waterborne diseases considerably influence human health and cause high mortality worldwide. Antibiotics have been known to treat bacterial strains, and their excessive use enhances bacterial resistance. Hence, there is a strong need to find other methods of water disinfection with more efficient microbial control. CNTs have shown strong antimicrobial properties due to their remarkable structure. Among waterborne diseases, typhoid fever, cholera, and dysentery can be mentioned, which significantly affect human health and are the cause of high mortality worldwide. Clean, pathogen-free drinking water is necessary for living organisms. Removing pathogens from contaminated water is an essential requirement for human health and the environment. The process of removing pathogens from water is difficult due to the fluctuating concentration of pathogens and the type of pathogens present in the incoming water. Chlorine, ozone, and chlorine dioxide are common disinfectants that can control microbial growth, but they have short-term reactivity and can be problematic due to the formation of toxic disinfection byproducts. Therefore, it is important to extend an alternative technique that can effectively improve the reliability of disinfection [16,121,122].

Brady et al. developed the first SWCNT filter as a PVDF microporous membrane filter for water disinfection via the removal and inactivation of viruses and bacteria from an aqueous medium. These nanofillers were found specifically effective against E. coli and S. aureus [18,123,124]. Ali et al. also disclosed surface functionalization and novel nanocomposites made of CNTs, iron oxide, titanium oxide, ferric oxides, and silver nanoparticles as promising agents for disinfection and decontamination of drinking water from E. coli, S. aureus, and P. aeruginosa [16,18,124,125].

The interaction opportunity of CNTs with bacterial cells and the antibacterial activity of CNTs are increased in higher dispersivity [126]. According to Liu et al., individually dispersed SWNTs in a Tween-20 saline solution have stronger antibacterial activity than SWNT beads. They hypothesized that individually dispersed SWNTs act as multiple mobile “nano-darts” in solution and constantly attack bacterial cells, leading to the disruption of bacterial cell integrity and causing cell death [27]. Polymer conjugation is another strategy to potentiate the antimicrobial activity of CNTs. Molecular weight, chemical composition, surface charge, and functional groups of polymers directly affect the bactericidal properties of CNTs [16,120].

External factors such as CNTs’ dosage, the culture medium, treatment time, and bacterial species are important. In recent research, bactericidal behavior was found to be dependent on incubation time. It has been observed that Gram-positive Bacillus subtilis showed more cell inactivation after longer incubation with SWNTs [123,127].

Lilly et al. also found that SWCNTs and conjugated SWCNT-H₂O₂ are both effective in the deactivation of B. anthracis spores in comparison to non-treated with MWCNT and/or unconjugated oxidizing agents such as H₂O_2, NaOCL at the same concentration. This phenomenon was explained through the synergistic antimicrobial effect of each component [16,128]. For example, Arias and Yang notified that SWCNTs functionalized with hydroxyl and carboxyl groups exhibited extremely strong antibacterial activity in Gram-positive and Gram-negative species while amine-functionalized SWCNTs were considerably less effective. Steric hindrance and less direct contact caused by the long amine-terminated chain were suggested as the reason for this huge difference in antimicrobial potency [16,129].

From a safety point of view, CNTs’ interaction with biological systems may give rise to allergy, cytotoxicity, DNA destruction, and protein malfunctions [130]. Different levels of toxicity would occur depending on the size, shape, length, diameter, surface coating, surface charges, stability, and dispersivity of CNTS and the tissue type and mode of interaction with human cells. Therefore, toxicity evaluation is very critical for the commercialization of CNTs as novel antimicrobial agents [131,132,133].

TiO₂ (Titanium oxide) is one of the most expensive and widely used photocatalysts with bactericidal properties. Researchers tend to design a combination of TiO₂ and ZnO (Zinc oxide) and semiconductors to achieve high photosensitivity, redox potential, and photocatalytic activity with lower cost and toxicity [134]. Researchers evaluated the step-by-step inactivation of E. coli by photocatalysis. They declare that bacterial cell membranes are damaged by the process of photocatalysis caused by oxidative stress. Carré et al. had similar results on the photocatalytic effect of lipids and proteins on the elimination of E. coli by photocatalysis. Siddiqi et al. reported that photo-excited ZnO nanoparticles diffusing through the cell wall would inactivate the cytoplasmic protein and carbohydrate via the release of ROS molecules. Takao et al. suggested that the presence of a peptidoglycan layer increases the bactericide effect of photocatalysis. Rodríguez-González et al. also described the existence of lesions in the bacterial cell wall caused by ROS molecules and metal particles [134]. Kerek et al. showed that the photocatalyst coating of graphene with TiO₂ and ZnO caused a significant (p < 0.001) reduction in pathogen numbers compared to the control. It is assumed that photocatalysis and titanium coatings of carbon-based material (CNTs and Graphene) would be a potential alternative to fighting antimicrobial resistance, which has significant bacterial reduction capacity against environmental pathogens [134].