Chimeric Antigen Receptor T-Cell Therapy: History
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Subjects: Cell & Tissue Engineering | Immunology | Medicine, Research & Experimental
Contributor:
Massimo Martino
,
Virginia Naso
,
Barbara Loteta
,
Filippo Antonio Canale
,
Marta Pugliese
,
Caterina Alati
,
Gerardo Musuraca
,
Davide Nappi
,
Anna Gaimari
,
Fabio Nicolini
,
Massimiliano Mazza
,
Sara Bravaccini
,
Daniele Derudas
,
Giovanni Martinelli
,
Claudio Cerchione

The treatment landscape for hematologic malignancies has changed since the recent approval of highly effective CAR-T. Chimeric antigen receptor T-cell therapy (CAR-T) is a type of immunotherapy in which a patient’s T cells are collected and genetically engineered to improve their ability to recognize and kill cancer cells. However, several issues are still unsolved and represent the challenges for the coming years. The lack of initial responses and early relapse are some hurdles to be tackled. Moreover, new strategies are needed to increase the safety profile or shorten the manufacturing process during CAR-T cells therapy production. Finally, the clinical experience with CAR-T cells for solid tumors has been less encouraging, and development in this setting is desirable

  • CAR-T
  • manufacturing
  • toxicities

1. Introduction

Chimeric antigen receptor T-cell therapy (CAR-T) is a type of immunotherapy in which a patient’s T cells, immune cells with anti-cancer activity, are collected and genetically engineered to improve their tropism and promote the elimination of cancer cells [1][2][3][4]. The modified cells are expanded in the laboratory and then returned to the patient to fight cancer. The year 2018 represents a milestone in the history of medicine: the Food and Drug Administration (FDA) [5][6] and the European Medicines Agency (EMA) [7] approved the first two products containing autologous T cells genetically modified ex vivo, tisagenlecleucel and axicabtageneciloleucel, for commercial use, revolutionizing the treatment landscape for relapsed or refractory (R/R) ALL and R/R non-Hodgkin lymphoma (NHL). However, history goes on, and in the last year, the FDA approved lisocabtagenemaraleucel in R/R NHL [8] and idecabtagenevicleucel in R/R multiple myeloma (MM) [9][10][11]. In addition, brexucabtageneautoleucel has been approved for treating adult patients with R/R mantle cell lymphoma [12] and is now being studied in patients with R/R B-ALL [13]. Moreover, the rolling submission of a biologics license application has been completed to support the approval of the investigational ciltacabtageneautoleucel in R/R MM [14]. Researchers also published data about an autologous CAR-T that uses a novel binding domain to target a B-cell maturation antigen (CARTddBCMA) in R/R MM, designed to reduce the risk of immunogenicity, while increasing stability [15]. The main characteristics of the constructs and the clinical indications are summarized in Table 1.
Table 1. Main characteristics of the CAR-T constructs and clinical indications.

Name

General Description

Therapeutic Indications

Tisagenlecleucel

Immunocellular therapy containing tisagenlecleucel, autologous T cells genetically modified ex vivo using a lentiviral vector encoding an anti-CD19 chimeric antigen receptor.

Pediatric and young adult patients up to and including 25 years of age with B-cell acute lymphoblastic leukemia that is refractory, in relapse post-transplant, or in second or later relapse.

Adult patients with R/R diffuse large B-cell lymphoma after two or more lines of systemic therapy.

Axicabtageneciloleucel

A CD19-directed genetically modified autologous T-cell immunotherapy. T cells are genetically modified ex vivo by retroviral transduction to express a chimeric antigen receptor comprising a murine anti-CD19 single-chain variable fragment linked to the CD28 co-stimulatory domain and CD3-zeta signaling domain.

After two or more lines of systemic therapy, adult patients with R/R diffuse large B-cell lymphoma and primary mediastinal large B-cell lymphoma.

Lisocabtagenemaraleucel

Anti-CD19 single-chain variable fragment (scFv) targeting domain for antigen specificity, a transmembrane domain, a 4-1BB costimulatory domain hypothesized to increase T-cell proliferation and persistence, and a CD3-zeta T-cell activation domain.

After two or more lines of systemic therapy, adult patients with R/R large B-cell lymphoma, including diffuse large B-cell lymphoma, not otherwise specified, high-grade B-cell lymphoma, primary mediastinal large B-cell lymphoma, and follicular lymphoma grade 3B.

Brexucabtageneautoleucel

Autologous peripheral blood T-lymphocytes (PBTL) that have been transduced with a retroviral vector expressing a chimeric antigen receptor (CAR) consisting of an anti-CD19 single-chain variable fragment (scFv) coupled to the zeta chain of the T-cell receptor (TCR)/CD3 complex (CD3 zeta) and the costimulatory signaling domain CD28.

Treatment of adult patients with R/R mantle cell lymphoma.

Idecabtagenevicleucel

Anti B-Cell maturation antigen (BCMA) scFv fused to the CD137 (4-1BB) co-stimulatory and CD3ζ signaling domains.

Adult patients with R/R multiple myeloma after four or more prior lines of therapy, including an immunomodulatory agent, a proteasome inhibitor, and an anti-CD38 monoclonal antibody.

Ciltacabtageneautoleucel

BCMA-targeted T-cell therapies are directed against two BCMA epitopes (VH1 and VH2) to confer improved affinity for BCMA-expressing cells.

Not authorized. Trials ongoing in R/R multiple myeloma to both immunomodulatory agents and proteasome inhibitors, or with at least three prior lines of therapy and previously exposed to anti-CD38 monoclonal antibody.
The approval by the FDA and EMA and the available therapies mentioned above have significantly impacted the treatment of several hematologic malignancies. Moreover, out of the 600 active trials, ongoing FDA and EMA estimate to approve 10–20 gene therapy products per year by 2025.

2. Improving Responses

With the longer follow-up reported, we are closer to answering the question of whether sustained remissions are possible with CAR-T cells monotherapy. Unfortunately, despite initially impressive in-depth responses, more than half of the patients experienced a relapse. The failure is primarily mediated by antigen escape and T-cell exhaustion. In total, 33% of the relapses in ZUMA-1 were CD19 negative. Similarly, the loss of BCMA expression contributes to disease relapse in MM .
One strategy to improve efficacy is finding novel targets and designing constructs targeting more than one antigen, such as the dual-targeting CD19/CD22 CAR in B-cell malignancies , dual CD19/CD20 CAR in lymphomas and leukemia [16], and CD19/BCMA-specific CAR in MM [17]. CD22-directed CAR-T cells have shown efficacy against leukemia [18] and lymphoma [19], representing the first alternative CAR target with comparable efficacy to CD19 CAR-T. Dual-targeting CARs or combination therapy may prevent relapses due to escape variants; therefore, they are the way forward.
Combining allo-SCT and CAR-T cells therapy is an attractive area of research to improve the outcomes further. Allo-SCT has been used in several clinical trials after CAR-T cells treatment and showed efficacy and favorable prognosis in R/R B-ALL [20][21]. Considering the high heterogeneity among and within patients, different CAR-T cell products, and continuously improved treatment strategies, consolidative allo-SCT cannot be generalized; however, it is recommended for high-risk r/r B-ALL patients with no history of allo-SCT and achieved MRD-negative CR after anti-CD19 CAR-T therapy [21]. Patients without risk factors could achieve long-term remissions with a continuous expression of the CAR in durable and functional CAR-T cells [22].

3. Safety Profile

With the available clinical algorithms, multidisciplinary staff must maintain competency in managing complications. Healthcare institutions must regularly review management guidelines based on current evidence, and host and product variables. Nurses play a fundamental role in educating caregivers and patients to identify and initiate early management of toxicities. Moreover, collaboration with the Department of Intensive Care is essential to tackle the most impactful complications.
A higher incidence of cytokine release syndrome (CRS) and immune cell-associated neurotoxicity syndrome (ICANS) could be associated with several factors, such as construct with a CD28 co-stimulatory domain [23], CD8+ CAR-T dose [24], bridging therapies in patients with the more aggressive disease [25], and the use of different grading systems to classify toxicity. The American Society for Blood and Marrow Transplantation criteria are the most objective and easy to use [26].
IL-6 released by macrophages and monocytes plays a significant role in the pathogenesis of toxicities, and extensive research efforts are focused on better understanding the mechanisms of these toxicities and defining strategies to mitigate them [27]. In addition, identifying biomarkers that are easily applicable in clinical practice will improve the treatment of post-CAR-T infusion toxicity [28].
Strategies with a risk-adapted earlier use of corticosteroids and tocilizumab to mitigate CRS are evolving [29]. The treatment of ICANS has largely been limited to supportive care and corticosteroids. More recently, some early clinical data investigated the use of Anakinra as a promising agent in the prevention and treatment of severe ICANS [30]. The investigation of adding a suicide gene that monoclonal antibodies may trigger, such as rituximab or cetuximab, or by using the CRISPR/Cas9 genome editing technology to clone a specific CAR construct to the T-cell receptor α constant (TRAC) locus resulting in uniform CAR expression and enhanced T-cell potency, needs to be considered in future CAR-T production [31]. Novel strategies are developing to interrupt these mechanisms without attenuating CAR-T efficacy. For example, the granulocyte-macrophage colony-stimulating factor (GM-CSF) is now known to promote the production of proinflammatory cytokines in CRS. Strategies to reduce GM-CSF production are being explored, such as editing out GM-CSF from effector CAR-T cells [32].
The long-term incidence of patients presenting with B-cell aplasia and, in general, patients with leukopenia, thrombocytopenia, and anemia, should not be underestimated. Therefore, future CAR constructs could target a clonally restricted B-cell marker, such as a kappa and lambda light chain of immunoglobulins, which could maintain anti-tumor activity without compromising humoral immunity, preventing B-cell aplasia [33]. Another strategy could be deleting the target antigen on normal hematopoietic stem cells, as recently shown by Kim et al. [34].

4. Manufacture Process

One of the future objectives must be the reduction of the production times of the CAR-T.
Possible solutions for reducing manufacturing time include novel manufacturing processes, decentralized manufacturing, and exploring novel non-viral vector approaches of genetic engineering with a faster turnaround time [35]. For example, Jackson et al. showed that it is possible to manufacture CAR-T cells within a hospital setting using a GMP-compliant closed system [36]. As a result, the authors reduced the turnaround cell manufacturing time to eight days, lowering the cost and expediting patient treatment. Such a fast turnaround time is one of the main advantages of local manufacturing over centralized CAR-T manufacturing strategies.
Research is now pushing toward the next generation of CAR-T therapies, allogenic or off-the-shelf treatments that can be mass-manufactured from a healthy donor (HD)’s cells and used for multiple patients [37]. Off-the-shelf therapies have the potential to treat 100 patients per batch of allogeneic CAR-T, similar to a drug product, and have the advantage of being available immediately with no risk of delay or failed manufacturing. Although this technology is still being developed, sights are already set on the manufacturing efficiency and how it would compare to autologous CAR-T therapy production. HD sources include peripheral blood mononuclear cells, “universal donors” such as umbilical cord blood, induced pluripotent stem cells, embryonic stem cells, or HLA-silenced CD34+ progenitor stem cells [38]. UCART19 is a product investigated in children and adults with R/R B-ALL, with a manageable safety profile in heavily pretreated pediatric and adult patients with R/R B-cell ALL. However, allogeneic cells risk immune rejection by host T cells and allo-reactivation of the CAR-T cells via the TCR receptor against host tissues, causing graft versus host disease (GVHD) [39]. Many trials are currently employing off-the-shelf products, including a few practices with gene-edited deletion of the surface TRAC molecule to prevent GVHD [40].

5. Solid Tumors

Several small trials on CAR-T cells in solid tumors have been conducted; however, the research is still in its early stages. Most of the data published so far are small, preliminary results of clinical trials. No findings have shown sustained, long remissions [41]. Several factors hinder the effectiveness of cell therapy against solid tumors. First, intra-tumor heterogeneity is one where cells of a solid tumor do not all present the same mix or sufficient expression for the antigens at their surfaces; thus, CAR-T cells could spare some cancer cells that will, later on, induce a relapse [42]. An immune suppressive tumor microenvironment (TME) is the second factor. Solid tumors can interfere with proper inflammation, producing immune-suppressing agents such as the checkpoint molecule PD-L1 that will prevent immune activation. The accessibility of CAR-T cells to the tumor mass is a third critical factor. A solid group of cells stacked in layers is difficult for T cells to infiltrate. While some trials deliver the cells systemically, others aim to improve efficacy by administering CAR-T cells directly to the tumor site [43].

Several studies are underway to combine CAR-T with cytokine administration, checkpoint blockade, oncolytic viruses, radiation, and vaccines [44]. In addition, investigators have explored the use of T cells to deliver viruses into tumors directly. For example, combining CAR-T infusion with the local delivery of an oncolytic adenovirus encoding RANTES and IL-15 in preclinical models has improved homing to and the persistence of CAR-T cells at tumor sites [44].

The composition of immune cells in the tumor microenvironment is an essential element for the heterogeneity of tumors, and creates interesting yet challenging complexities when investigating dynamic interactions between cancer and immune cells [45]. Tumor transcriptomics data are informative; however, they do not immediately indicate immune cell compositions, which require computational inference. The computational algorithms are based on two categories: deconvolution approaches and gene signature [46]. Deconvolution methods define the problem as mathematical equations that model the gene expression of a tissue sample as the weighted sum of the expression profiles from the cells in the population mix. Gene signature-based approaches utilize a list of cell-type-specific gene sets. These two complementary categories of algorithms have demonstrated variable performance advantages in estimating specific immune cell types in different tumors [47]. The algorithms could help the user gain more comprehensive and robust results with CAR-T cells.

6. Cost

Since the first CAR-T cells therapies gained FDA approval in 2017, the one-time treatments have led to unprecedented response rates in patients with R/R lymphoid malignancies, with remarkable price tags of about $373,000 for a single infusion.

In the late 1980s, the Italian hospitals developed a calculating tariff method based on diagnosis-related groups (DRG)[48]. The DRGs have also been applied in North America [49]. DRGs are awarded by a “grouper” program based on International Classification of Diseases diagnoses, physical characteristics (gender, age), procedures, the presence of complications or comorbidities, and discharge status. By the DRG identified and the length of hospital stay, the region pays the cost of hospitalization. DRGs include all the actions necessary to treat and diagnose the patient for each treatment because patients within each category are clinically similar and are expected to employ the same level of hospital resources (fixed price).

According to DRG, CAR-T cells therapy in Italy is remunerated at $59,806 in most authorized centers. However, the actual repayment of the DRG does not correspond to the cost of the CAR-T cells procedure and, in general, of a transplant procedure [68]. Furthermore, the price does not account for manufacturing the product, managing potential long-term complications, or managing other therapy lines after relapse. The DRG model finds complex applications in this scenario, and maybe a novel model should be explicitly applied for cell therapies. For example, activity-based costing (ABC) is a tool developed to improve efficiency and control cost. The procedure is based on the concept that the production of a product or the performance of a service spends activities that consume resources [50] [51]. ABC endeavors to assign costs to each of these activities, and resources so that total costs can be better understood and administrated. Finally, the pharmaceutic company introduced an outcome-based pricing model: if the treatment does not work, no one pays for it. That proposal is exciting with many problems, the least of which is that the definition of “not working” is unclear [48].

7. Conclusions

CAR-T cells therapy has shown unprecedented results in patients without curative options. Future work focusing on target identification, toxicity management, and manufacturing time shortening will broaden this exciting therapy’s clinical applicability and sustainability, with more prolonged remissions without additional treatment.

This entry is adapted from the peer-reviewed paper 10.3390/ijms232113332

References

  1. Kamb, A.; Go, W.Y. Cancer T-cell therapy: Building the foundation for a cure. F1000Research 2020, 9, 1295.
  2. Martino, M.; Alati, C.; Canale, F.; Musuraca, G.; Martinelli, G.; Cerchione, C. A Review of Clinical Outcomes of CAR T-Cell Therapies forB-Acute Lymphoblastic Leukemia. Int. J. Mol. Sci. 2021, 22, 2150.
  3. Martino, M.; Canale, F.A.; Alati, C.; Vincelli, I.D.; Moscato, T.; Porto, G.; Loteta, B.; Naso, V.; Mazza, M.; Nicolini, F.; et al. CART-Cell Therapy: Recent Advances and New Evidence in Multiple Myeloma. Cancers 2021, 13, 2639.
  4. Schepisi, G.; Cursano, M.C.; Casadei, C.; Menna, C.; Altavilla, A.; Lolli, C.; Cerchione, C.; Paganelli, G.; Santini, D.; Tonini, G.; et al. CAR-T cell therapy: A potential new strategy against prostate cancer. J. Immunother. Cancer 2019, 7, 258.
  5. FDA Approves Tisagenlecleucel for Adults with Relapsed or Refractory Large B-Cell Lymphoma. Available online: https://www.fda.gov/drugs/resources-information-approved-drugs/fda-approves-tisagenlecleucel-adults-relapsed-or-refractory-large-b-cell-lymphoma (accessed on 5 March 2018).
  6. FDA Approves Axicabtagene Ciloleucel for Large B-Cell Lymphoma. Available online: https://www.fda.gov/drugs/resources-information-approved-drugs/fda-approves-axicabtagene-ciloleucel-large-b-cell-lymphoma (accessed on 9 August 2022).
  7. First Two CAR-T Cell Medicines Recommended for Approval in the European Union. Available online: https://www.ema.europa.eu/en/news/first-two-car-t-cell-medicines-recommended-approval-european-union (accessed on 9 August 2022).
  8. FDA Approves Lisocabtagene Maraleucel for Relapsed or Refractory Large B-Cell Lymphoma. Available online: https://www.fda.gov/drugs/resources-information-approved-drugs/fda-approves-lisocabtagene-maraleucel-relapsed-or-refractory-large-b-cell-lymphoma (accessed on 9 August 2022).
  9. FDA Approves Idecabtagene Vicleucel for Multiple Myeloma. Available online: https://www.fda.gov/drugs/resources-information-approved-drugs/fda-approves-idecabtagene-vicleucel-multiple-myeloma (accessed on 9 August 2022).
  10. Chen, Y.; Nagarajan, C.; Tan, M.S.; Martinelli, G.; Cerchione, C. BCMA-targeting approaches for treatment of multiple myeloma. Panminerva Medica 2021, 63, 28–36.
  11. Juan, M. CAR T cells targeting options in the fight against multiple myeloma. Panminerva Medica 2021, 63, 37–45.
  12. FDA Approves Brexucabtagene Autoleucel for Relapsed or Refractory Mantle Cell Lymphoma. Available online: https://www.fda.gov/drugs/resources-information-approved-drugs/fda-approves-brexucabtagene-autoleucel-relapsed-or-refractory-mantle-cell-lymphoma (accessed on 9 August 2022).
  13. Shah, B.D.; Ghobadi, A.; Oluwole, O.O.; Logan, A.; Boissel, N.; Cassaday, R.D.; Forcade, E.; Bishop, M.R.; Topp, M.S.; Tzachanis, D.; et al. Phase 2 results of the ZUMA-3 study evaluating KTE-X19, an anti-CD19 chimeric antigen receptor (CAR) T-cell therapy, in adult patients (pts) with relapsed/refractory B-cell acute lymphoblastic leukemia (R/R B-ALL). J. Clin. Oncol. 2021, 39, 7002.
  14. Berdeja, J.G.; Madduri, D.; Usmani, S.Z.; Jakubowiak, A.; Agha, M.; Cohen, A.D.; Stewart, A.K.; Hari, P.; Htut, M.; Lesokhin, A.; et al. Ciltacabtagene autoleucel, a B-cell maturation antigen-directed chimeric antigen receptor T-cell therapy in patients with relapsed or refractory multiple myeloma (CARTITUDE-1): A phase 1b/2 open-label study. Lancet 2021, 398, 314–324.
  15. Frigault, M.J.; O’Donnell, E.; Raje, N.S.; Cook, D.; Yee, A.; Rosenblatt, J.; Gibson, C.; Logan, E.; Avigan, D.; Bishop, M.R.; et al. Phase 1 Study of CART-ddBCMA, a CAR-T therapy utilizing a novel synthetic binding domain, for the treatment of subjects with relapsed and refractory multiple myeloma. J. Clin. Oncol. 2021, 39, 8015.
  16. Shah, N.N.; Johnson, B.D.; Schneider, D.; Zhu, F.; Szabo, A.; Keever-Taylor, C.A.; Krueger, W.; Worden, A.A.; Kadan, M.J.; Yim, S.; et al. Bispecific anti-CD20, anti-CD19 CAR T cells for relapsed B cell malignancies: A phase 1 dose escalation and expansion trial. Nat. Med. 2020, 26, 1569–1575.
  17. Yan, L.; Qu, S.; Shang, J.; Shi, X.; Kang, L.; Xu, N.; Zhu, M.; Zhou, J.; Jin, S.; Yao, W.; et al. Sequential CD19 and BCMA-specific CAR T-cell treatment elicits sustained remission of relapsed and/or refractory myeloma. Cancer Med. 2020, 10, 563–574.
  18. Killock, D. Anti-CD22 CAR T cells in ALL. Nat. Rev. Clin. Oncol. 2020, 17, 391.
  19. Baird, J.H.; Frank, M.J.; Craig, J.; Patel, S.; Spiegel, J.Y.; Sahaf, B.; Oak, J.S.; Younes, S.F.; Ozawa, M.G.; Yang, E.; et al. CD22-directed CAR T-cell therapy induces complete remissions in CD19-directed CAR–refractory large B-cell lymphoma. Blood 2021, 137, 2321–2325.
  20. Zhao, Y.-L.; Liu, D.-Y.; Sun, R.-J.; Zhang, J.-P.; Zhou, J.-R.; Wei, Z.-J.; Xiong, M.; Cao, X.-Y.; Lu, Y.; Yang, J.-F.; et al. Integrating CAR T-Cell Therapy and Transplantation: Comparisons of Safety and Long-Term Efficacy of Allogeneic Hematopoietic Stem Cell Transplantation After CAR T-Cell or Chemotherapy-Based Complete Remission in B-Cell Acute Lymphoblastic Leukemia. Front. Immunol. 2021, 12, 605766.
  21. Zhang, M.; Huang, H. How to Combine the Two Landmark Treatment Methods—Allogeneic Hematopoietic Stem Cell Transplantation and Chimeric Antigen Receptor T Cell Therapy Together to Cure High-Risk B Cell Acute Lymphoblastic Leukemia? Front. Immunol. 2020, 11, 611710.
  22. Jacoby, E. The role of allogeneic HSCT after CAR T cells for acute lymphoblastic leukemia. Bone Marrow Transplant. 2019, 54, 810–814.
  23. Lu, P.; Lu, X.-A.; Zhang, X.; Xiong, M.; Zhang, J.; Zhou, X.; Qi, F.; Yang, J.; He, T. Which is better in CD19 CAR-T treatment of r/r B-ALL, CD28 or 4-1BB? A parallel trial under the same manufacturing process. J. Clin. Oncol. 2018, 36, 3041.
  24. DeAngelo, D.; Ghobadi, A.; Park, J.; Dinner, S.; Mannis, G.; Lunning, M.; Khaled, S.; Fathi, A.; Gojo, I.; Wang, E.; et al. Clinical outcomes for the phase 2, single-arm, multicenter trial of JCAR015 in adult B-ALL (ROCKET study). J. Immunother. Cancer 2017, 5 (Suppl. S2).
  25. Nasta, S.D.; Hughes, M.E.; Namoglu, E.C.; Landsburg, D.J.; Chong, E.A.; Barta, S.K.; Frey, N.V.; Gerson, J.N.; Maity, A.; Plastaras, J.; et al. A Characterization of Bridging Therapies Leading up to Commercial CAR T-Cell Therapy. Blood 2019, 134, 4108.
  26. Lee, D.W.; Santomasso, B.D.; Locke, F.L.; Ghobadi, A.; Turtle, C.J.; Brudno, J.N.; Maus, M.V.; Park, J.H.; Mead, E.; Pavletic, S.; et al. ASTCT Consensus Grading for Cytokine Release Syndrome and Neurologic Toxicity Associated with Immune Effector Cells. Biol. Blood Marrow Transplant. 2019, 25, 625–638.
  27. Siegler, E.L.; Kenderian, S.S. Neurotoxicity and Cytokine Release Syndrome After Chimeric Antigen Receptor T Cell Therapy: Insights into Mechanisms and Novel Therapies. Front. Immunol. 2020, 11, 1973.
  28. Hong, R.; Hu, Y.; Huang, H. Biomarkers for Chimeric Antigen Receptor T Cell Therapy in Acute Lymphoblastic Leukemia: Prospects for Personalized Management and Prognostic Prediction. Front. Immunol. 2021, 12, 627764.
  29. Banerjee, R.; Marsal, J.; Huang, C.-Y.; Lo, M.; Kambhampati, S.; Kennedy, V.E.; Arora, S.; Wolf, J.L.; Martin, T.G.; Wong, S.W.; et al. Early Time-to-Tocilizumab after B Cell Maturation Antigen-Directed Chimeric Antigen Receptor T Cell Therapy in Myeloma. Transplant. Cell. Ther. 2021, 27, 477.e1–477.e7.
  30. Munugala, N.; Dashkevych, U.; Husnain, M. Role of anakinra in the management of icans after CAR T-cell therapy for lymphoma. J. Clin. Oncol. 2022, 40, e19506.
  31. Eyquem, J.; Mansilla-Soto, J.; Giavridis, T.; van der Stegen, S.J.C.; Hamieh, M.; Cunanan, K.M.; Odak, A.; Gönen, M.; Sadelain, M. Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection. Nature 2017, 543, 113–117.
  32. Sachdeva, M.; Duchateau, P.; Depil, S.; Poirot, L.; Valton, J. Granulocyte–macrophage colony-stimulating factor inactivation in CAR T-cells prevents monocyte-dependent release of key cytokine release syndrome mediators. J. Biol. Chem. 2019, 294, 5430–5437.
  33. Ramos, C.A.; Savoldo, B.; Torrano, V.; Ballard, B.; Zhang, H.; Dakhova, O.; Liu, E.; Carrum, G.; Kamble, R.T.; Gee, A.P.; et al. Clinical responses with T lymphocytes targeting malignancy-associated k light chains. J. Clin. Investig. 2016, 126, 2588–2596.
  34. Kim, M.Y.; Yu, K.-R.; Kenderian, S.S.; Ruella, M.; Chen, S.; Shin, T.-H.; Aljanahi, A.A.; Schreeder, D.; Klichinsky, M.; Shestova, O.; et al. Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia. Cell 2018, 173, 1439–1453.e19.
  35. Yang, J.; He, J.; Zhang, X.; Wang, Z.; Zhang, Y.; Cai, S.; Sun, Z.; Ye, X.; He, Y.; Shen, L.; et al. A Feasibility and Safety Study of a New CD19-Directed Fast CAR-T Therapy for Refractory and Relapsed B Cell Acute Lymphoblastic Leukemia. Blood 2019, 134 (Suppl. S1), 825.
  36. Jackson, Z.; Roe, A.; Sharma, A.A.; Lopes, F.B.T.P.; Talla, A.; Kleinsorge-Block, S.; Zamborsky, K.; Schiavone, J.; Manjappa, S.; Schauner, R.; et al. Automated Manufacture of Autologous CD19 CAR-T Cells for Treatment of Non-hodgkin Lymphoma. Front. Immunol. 2020, 11, 1941.
  37. Benjamin, R.; Graham, C.; Yallop, D.; Jozwik, A.; Mirci-Danicar, O.C.; Lucchini, G.; Pinner, D.; Jain, N.; Kantarjian, H.; Boissel, N.; et al. Genome-edited, donor-derived allogeneic anti-CD19 chimeric antigen receptor T cells in paediatric and adult B-cell acute lymphoblastic leukaemia: Results of two phase 1 studies. Lancet 2020, 396, 1885–1894.
  38. Townsend, M.H.; Bennion, K.; Robison, R.A.; O’Neill, K.L. Paving the way towards universal treatment with allogenic T cells. Immunol. Res. 2020, 68, 63–70.
  39. Qasim, W. Allogeneic CAR T cell therapies for leukemia. Am. J. Hematol. 2019, 94, S50–S54.
  40. Poirot, L.; Philip, B.; Schiffer-Mannioui, C.; Le Clerre, D.; Chion-Sotinel, I.; Derniame, S.; Potrel, P.; Bas, C.; Lemaire, L.; Galetto, R.; et al. Multiplex Genome-Edited T-cell Manufacturing Platform for “Off-the-Shelf” Adoptive T-cell Immunotherapies. Cancer Res. 2015, 75, 3853–3864.
  41. Wagner, J.; Wickman, E.; DeRenzo, C.; Gottschalk, S. CAR T Cell Therapy for Solid Tumors: Bright Future or Dark Reality? Mol. Ther. 2020, 28, 2320–2339.
  42. 63. Marofi, F.; Motavalli, R.; Safonov, V.A.; Thangavelu, L.; Yumashev, A.V.; Alexander, M.; Shomali, N.; Chartrand, M.S.; Pathak, Y.; Jarahian, M.; et al. CAR T cells in solid tumors: Challenges and opportunities. Stem Cell Res. Ther. 2021, 12, 81. https://doi.org/10.1186/s13287-020-02128-1.
  43. Hanahan, D.; Coussens, L.M. Accessories to the crime: Functions of cells recruited to the tumor microenvironment. Cancer Cell 2012, 21, 309–322. https://doi.org/10.1016/j.ccr.2012.02.022.
  44. Nishio, N.; Diaconu, I.; Liu, H.; Cerullo, V.; Caruana, I.; Hoyos, V.; Bouchier-Hayes, L.; Savoldo, B.; Dotti, G. Armed Oncolytic Virus Enhances Immune Functions of Chimeric Antigen Receptor–Modified T Cells in Solid Tumors. Cancer Res. 2014, 74, 5195–5205. https://doi.org/10.1158/0008-5472.can-14-0697.
  45. Mc Granahan, N.; Swanton, C. Clonal heterogeneity and tumor evolution: Past, present, and the future. Cell 2017, 168, 613–662
  46. Finotello, F.; Trajanoski, Z. Quantifying tumor-infiltrating immune cells from transcriptomics data. Cancer Immunol. Immu-nother. 2018, 67, 1031–1040
  47. Li, T.; Fu, J.; Zeng, Z.; Cohen, D.; Li, J.; Chen, Q.; Li, B.; Liu, X.S. TIMER2.0 for analysis of tumor-infiltrating immune cells. Nu-cleic Acids Res. 2020, 48, W509–W514. https://doi.org/10.1093/nar/gkaa407
  48. Mistichelli, J. Diagnosis Related Groups (DRGs) and the Prospective Payment System: Forecasting Social Implications; Georgetown University: Washington, DC, USA, 1984.
  49. Fetter, R.B.; Freeman, J.L. Diagnosis-related groups: Product line management within hospitals. Acad. Manag. Rev. 1986, 11, 41–54.
  50. Martino, M.; Console, G.; Russo, L.; Meliado’, A.; Meliambro, N.; Moscato, T.; Irrera, G.; Messina, G.; Pontari, A.; Morabito, F. Autologous Stem Cell Transplantation in Patients with Multiple Myeloma: An Activity-based Costing Analysis, Comparing a Total Inpatient Model Versus an Early Discharge Model. Clin. Lymphoma Myeloma Leuk. 2017, 17, 506–512. https://doi.org/10.1016/j.clml.2017.05.018.
  51. Cooper, R.; Kaplan, R.S. The promise--and peril--of integrated cost systems. Harv. Bus. Rev. 1998, 76, 109–119.
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