1. Autophagy in Cell Survival

The early concept that autophagy can serve as a pro-survival pathway came from the observation that autophagy is regulated by nutrient conditions and is increased in response to a reduction in the glucose or amino acid level in cells or tissues ^[1][2]. As the research tools and model organisms used to study autophagy expanded, starvation-induced autophagy was observed across a wide range of eukaryotic organisms ^[3]. The importance of autophagy for survival can be most easily demonstrated with a single-cell organism such as yeast; autophagy-defective yeast mutants display a tremendous decrease in viability under starvation conditions ^[4]. Similarly, atg5 knockout (KO) mice exhibit nutrient and energy insufficiency shortly after birth, accompanied by a significantly lower amino acid concentration in plasma and tissues despite their almost normal appearance upon birth. The early death of these mice can be rescued by milk ingestion ^[5].

Why is autophagy important for survival at the cellular level? Autophagy (or the autophagy machinery) facilitates cell survival in canonical and non-canonical ways. In canonical autophagy, two fundamental results are achieved: the degradation and recycling of cytoplasmic components. Although these two results are almost always coupled during the autophagy process, the cell could have a more urgent need for one versus the other under a given condition. For example, the cell can use selective autophagy to degrade unfavorable components, such as damaged organelles and pathogens. In these cases, the cell uses autophagy mainly for clearance (to prevent certain cellular components from causing further harm), but not for the degradation products gained from this process. In contrast, during nitrogen or amino acid starvation, the cell needs to acquire amino acids by degrading and recycling existing materials in the cell via autophagy. In these cases, it is the recycling function that is more important to the cell, and this is the main function of autophagy that most people envision when considering this process. In the following paragraphs, we discuss the applications of these two functions of autophagy. However, in addition to its canonical role in degradation, the autophagy machinery also facilitates cell survival in non-canonical ways that do not require the degradation step.

1.1. Autophagy for Clearance

Damaged macromolecules and organelles are generated over time and accumulate over the lifetime of the cell. If left unchecked, the accumulation of these components can lead to serious problems, such as DNA damage. By clearing these constituents, autophagy prevents them from causing further harm to the cell and thus facilitates cell survival. This clearance function of autophagy can be critical in terminally differentiated, non-dividing cells, such as neurons ^[6][7]. Both nonselective and selective autophagy promotes clearance of cytoplasmic components.

One example of autophagy in clearance is the removal of oxidized biomolecules (proteins, DNA, and lipids) when the cells are under oxidative stress ^[8]. Many signaling pathways, including those involving AMPK, MTOR, NFKB/NF-κB, HIF1A/HIF-1, and more, have been proposed to induce autophagy in response to oxidative stress ^{[8][9][10][11]}. Oxidative stress can also regulate autophagy more directly. Oxygen availability can affect the activity of KDM5C, which is responsible for the demethylation of ULK1. Upon hypoxia, the activity of KDM5C is reduced due to lack of oxygen, leading to the accumulation of symmetrical dimethylation at arginine 170 of ULK1. This modification of ULK1 activates its kinase activity, and thus increases autophagy flux ^[12].

Selective autophagy facilitates the turnover of damaged or superfluous organelles. For example, damaged mitochondria can lead to excessive reactive oxygen species, insufficient ATP supply, and/or apoptosis. In differentiated cells that cannot refresh their mitochondrial pool by cell division, mitophagy is proposed to be an important way to regulate mitochondria quality control and maintain homeostasis ^{[6][7][13][14][15][16]}. The accumulation of dysfunctional mitochondria has been hypothesized as one of the potential causes of Parkinson’s disease ^[17][18]. Autophagy can also participate in organelle homeostasis in response to environmental changes. For example, peroxisome biogenesis can be induced when yeast cells are incubated with oleic acid or methanol. If yeast cells are then provided with a preferred carbon source, such as glucose, cells use pexophagy (selective autophagy of peroxisomes) to degrade the excess peroxisomes ^[19][20].

In addition to its role in stress response, selective autophagy plays an integral part in development. For example, during yeast meiosis, the level of Atg40, the reticulophagy (selective autophagy of the endoplasmic reticulum) receptor, is induced, and reticulophagy is activated to remove a subset of this organelle. The loss of Atg40 and most of the Atg proteins leads to decreased sporulation efficiency ^[21]. In mammals, mitophagy is responsible for the clearance of mitochondria during the maturation of erythroid cells, and blocking mitophagy leads to reduced mature erythrocytes, causing anemia as well as shorter lifespan in mice ^[22][23]. The inherent roles of selective autophagy in development suggest a widespread role of this mode of autophagy.

Autophagy can also clear intracellular protein aggregates and pathogenic microbes. For example, ubiquitinated protein aggregates in yeast cells can be recognized by the receptor Cue5 via its ubiquitin-binding CUE domain, and its Atg8-family interacting motif/AIM recruits Atg8–PE. Recent research identified CCT2 as the autophagy receptor for polyQ-HTT (huntingtin) protein aggregates in mammalian cells ^[24]. Receptors, such as SQSTM1 and OPTN in mammalian cells, possess both a ubiquitin-binding domain and LC3-interacting region/LIR. These and other receptors are involved in the selective autophagic degradation of intracellular pathogens via xenophagy ^[25][26].

By fine-tuning selective autophagy, the cell can sequester/degrade different cargoes in response to different signals or stress. One of the many ways to achieve this is through the regulation of autophagy receptors. For example, the iron level in the cell can be regulated by ferritin, which can store and release iron. The level of NCOA4, the autophagy receptor for ferritinophagy (selective autophagy of ferritin), is regulated by ubiquitin-dependent proteasomal degradation in an iron-dependent manner. In response to iron depletion, NCOA4 is stabilized, facilitating the autophagic degradation of ferritin and hence iron release ^[27][28]. As will be described later in this review, mitochondria damage enhances the mitochondrial recruitment of mitophagy receptors, such as OPTN, to initiate mitophagy ^[7]. Importantly, mitophagy removes damaged mitochondria under mild stress and thus prevents intrinsic apoptosis mediated by the release of mitochondrial contents, such as CYCS/cytochrome c, into the cytosol, which is addressed later on in this review.

Although the sequestration and subsequent degradation of cargoes are almost always coupled in autophagy, temporal homeostasis could be restored solely by the sequestration step as it sequesters the unfavorable compartments from the cytoplasm, or the damaged parts from an organelle. This can be considered as an extension of the clearance role of autophagy. For example, it was reported that the clearance of dysfunctional parts of the endoplasmic reticulum (ER) via sequestration within an autophagosome may be sufficient to temporarily restore homeostasis without the subsequent degradation of the cargo ^[29]. Similarly, the sequestration of damaged parts of mitochondria into autophagosomes could prevent harm from excess reactive oxygen species without relying on the breakdown of the organelle.

1.2. Autophagy for Recycling

The recycling function of autophagy provides cells with raw material for synthesizing new molecules and producing energy. The degradation and recycling of cytoplasm are especially important when cells are experiencing stress, and are in nutrient-limiting conditions; however, it is critical to regulate autophagy to avoid excessive degradation that can lead to cell death. The regulation of autophagy under normal and stress conditions has been extensively studied, and there are excellent reviews that cover this topic ^{[30][31][32][33]}. Here, we briefly provide two examples of how the cell coordinates autophagy for its pro-survival role in response to stress, as well as the major regulatory pathways involved.

The target of rapamycin (TOR) pathway is one of the key regulators for autophagy in response to nutrient stress ^[31][34]. When nutrients are abundant, TOR complex 1 (TORC1/MTORC1) is active. In yeast, phosphorylation of Atg1 and Atg13 by active TORC1 inhibits the activity of the Atg1 complex. During nutrient starvation, TORC1 is inactivated, preventing the inhibitory phosphorylation of Atg1 and Atg13 and leading to increased autophagy induction. Similar to TORC1, mammalian MTORC1 can phosphorylate and inhibit proteins that participate in autophagy, including ULK1. TORC1/MTORC1 also regulates autophagy transcriptionally by directly or indirectly regulating the activity or localization of transcriptional regulators for ATG genes, underscoring the complexity of autophagy regulation in response to nutrient levels ^[37][39][40]. The regulation of TORC1/MTORC1 on autophagy also extends to other aspects, such as the mRNA stability of ATG genes and histone modification ^[37][39][40].

The AMP-activated protein kinase (AMPK) pathway promotes autophagy in response to energy stress. AMPK-mediated induction of autophagy facilitates the turnover of macromolecules to provide substrates for catabolic pathways ^[35]. AMPK is activated when the cellular AMP:ATP ratio is high ^[36][37][38]. Activated AMPK phosphorylates and activates many autophagy-related proteins, including ULK1, BECN1 (a homolog of yeast Vps30/Atg6), ATG9A, and subunits of the class III PtdIns3K complex. AMPK also inhibits the formation of other different PIK3C3/VPS34-containing complexes involved in autophagy-independent processes ^[39]. In addition, AMPK stimulates autophagy by mediating the upregulation of autophagy-related genes through the phosphorylation of transcription factors. For example, AMPK phosphorylates FOXO3 (forkhead box O3), promoting its nuclear translocation and transcriptional activity; FOXO3 target genes include ULK1, ATG4B, ATG14, ATG12, BECN1, and BNIP3. AMPK can also suppress MTOR activity, which, as described above, can lead to an increase in autophagy ^[39][41][42].

2. Pro-Survival Roles of the Autophagy Machinery Independent of Clearance or Vacuolar/Lysosomal Degradation

In addition to its primary role in degradation, the autophagy machinery performs multiple tasks. From a general viewpoint, the autophagy machinery can (1) recognize specific cargoes, (2) partition the cargo(es) into a double-membrane structure, and (3) deliver the double-membrane structure to the vacuole/lysosome. In addition to degradation, this process can also be viewed as a general partitioning and delivery system in the cell. In the following paragraphs, we describe the role of the autophagy machinery in the cytoplasm-to-vacuole targeting (Cvt) pathway and secretory autophagy. These pathways expand the pro-survival function of the autophagy machinery.

2.1. The Cvt Pathway

Most resident vacuolar proteins are delivered to this organelle through a portion of the traditional secretory pathway, from the ER to the Golgi apparatus and then being diverted to endosomes and the vacuole. Yeast cells can also utilize autophagy to deliver proteins in the cytosol to the vacuole through the Cvt pathway. The Cvt pathway is biosynthetic, taking enzymes from their site of synthesis in the cytosol to their site of function, the vacuole, but it essentially uses the autophagy machinery for transport ^[40][41]. During this process, specific vacuolar enzymes are recognized by autophagy receptors, followed by the recruitment of core autophagy machinery for sequestration within a double-membrane Cvt vesicle; as with other types of selective autophagy, the Cvt vesicle is closely apposed to the cargo (e.g., precursor aminopeptidase I [prApe1]) and excludes bulk cytoplasm. The completed Cvt vesicle fuses with the vacuole to deliver the cargo, which in this case is not degraded, but may be subject to proteolytic activation. The cargo specificity in the Cvt pathway is accomplished by specific cargo receptors. For example, Atg19 is the receptor for the hydrolases Ams1, prApe1, and Ape4. An autophagic mechanism thus serves as a delivery system in the Cvt pathway, ensuring proper vacuole function ^[40]. The discovery of the Cvt pathway is an important complement to the understanding of the function of the autophagy machinery. While this pathway is not found in mammalian cells, the secretory autophagy pathway, as described below, is similar to the Cvt pathway, as both pathways selectively sequester specific cargoes from the cytoplasm and deliver them to a specific destination.

2.2. Secretory Autophagy

Secretory autophagy utilizes both the partitioning and delivery functions of autophagy. While many secreted proteins rely on the signal sequence at their N terminus to enter the ER and follow the conventional ER–Golgi secretory pathway, for secreted proteins that lack a signal sequence, unconventional secretion pathways are used ^[42][43]. One such pathway is secretory autophagy, in which cytoplasmic secretory cargoes are engulfed within autophagosomes, which then fuse with the plasma membrane instead of lysosomes. One of the advantages of secretory autophagy is that it can secrete proteins directly from the cytoplasm ^[44][45].

One of the well-established cargoes identified in secretory autophagy is IL1B (interleukin 1 beta). IL1B is subject to sequestration in LC3-positive vesicles, followed by the fusion of such vesicles with the plasma membrane ^[46]. IL1B is recruited to phagophores via its interaction with TRIM16. TRIM16 interacts with the R-SNARE SEC22B for its recruitment to LC3-II⁺ sequestration membranes ^[47]. SEC22B on the secretory autophagosome interacts with SNARE proteins SNAP23, SNAP29, and STX3 (syntaxin 3) to allow fusion with the plasma membrane to accomplish the secretion of IL1B ^[47]. SEC22B may have a critical role in directing the secretory autophagosome away from the degradative pathway, causing it to fuse with the plasma membrane instead of a lysosome ^[45][47].

Caution should be taken when using the term “secretory autophagy”. Sometimes this term is confused with the term “autophagy-dependent secretion” ^[45]; however, these two processes are not necessarily identical. For example, the secretion of some cargoes depends on autophagy, but does involve autophagosomes; rather, autophagy degrades molecules that suppress their secretion. This distinction is especially important when interpreting genetic interactions between autophagy and the secretion of a cargo. Despite this caveat, it is important to acknowledge that the secretion of some aggregation-prone proteins is affected by autophagy, including amyloid beta (Aβ), and SNCA/α-synuclein, further emphasizing the role of autophagy in cell survival ^[44][45].

In addition to its relatively well-characterized role in the Cvt pathway and secretory autophagy, the autophagy machinery (or autophagy genes) has been implicated in many other cellular events, ranging from DNA repair to cell division to T-cell differentiation. A recent review provides a more comprehensive description of the non-canonical functions of the autophagy machinery ^[48], which are becoming a new research hotspot ^[49][50][51].

3. Autophagy in Cell Death

Autophagy is associated with cell death originally because of electron microscopy-based observations of autophagic structures in dying cells ^[52][53]. Some of these reports have been controversial as the observation of autophagy proximate to cell death could be due to a pro-survival activation of autophagy (antagonizing cell death) instead of a pro-death role ^[54][55][56]. Continued research has confirmed the participation of autophagy in cell death progression in Dictyostelium discoideum, Caenorhabditis elegans, Drosophila, and mammalian cell lines ^[55]. For example, mutation in many autophagy genes in C. elegans, including atg3, atg2, or atg5, reduces germ cell death induced by gamma radiation ^[57]. Similarly, autophagy is required for cell death induced by treatment with chemotherapeutic drugs in Bax- and Bak1-deficient mouse embryonic fibroblasts ^[58]. Although the specifics of the experimental manipulation may limit the interpretation in regard to the physiological role of autophagy in cell death, this research not only indicates the capability of the autophagy process in participating in cell death, but also provides insight into the role of autophagy under pathologically relevant conditions.

Autophagy-Dependent Cell Death

Regulated cell death (RCD) refers to cell death caused by genetically encoded mechanisms for the elimination of cells that are irreversibly damaged, superfluous, and/or potentially harmful, or for the programmed elimination of cells during development. RCD operates at the level of an organism or a colony to eliminate useless or (potentially) harmful cells, or allow dying cells to release molecules that alert the organism or colony about a threat ^[56]. Recent research has shown that autophagy plays an essential or facilitating role in multiple types of RCD. According to the Nomenclature Committee on Cell Death, autophagy-dependent cell death (ACD) is defined as a type of RCD that relies on the autophagic machinery or components thereof. In addition to autophagy-dependent cell death, autophagy also facilitates the process of ferroptosis, FAS-driven extrinsic RCD, necroptosis, and autosis (a specific instance of autophagy-dependent cell death) ^[56].

Many findings of ACD are obtained from studies on development, as these studies circumvent the caveats of human intervention, such as using RCD-inducing drugs. The regulated cell death of Drosophila salivary glands and midgut cells during development are well-established examples of ACD. The RCD of Drosophila salivary glands is reduced when either apoptosis or autophagy is blocked. The removal of salivary glands is further delayed when both RCD and autophagy are delayed, indicating that both processes are functional in this event ^[59]. The developmental RCD of Drosophila midgut can be severely delayed by mutation or knockdown of Atg1, Atg2, or Atg18, but not by inhibition of apoptosis ^[60]. Consistent with autophagy playing a role during salivary or midgut RCD, the expression of many Atg genes is upregulated during these two processes ^[60][61]. An increased number of GFP-Atg8a puncta (corresponding to autophagosomes, Atg8a is the functional Atg8 paralog in Drosophila melanogaster) under confocal microscopy, as well as an increased number of autolysosome-like structures under transmission electron microscopy, are observed during developmental RCD of Drosophila midgut ^[62].

Genetic studies using RNAi-mediated screening indicate that there is a deviation in the use of enzymes in ACD of Drosophila midgut from the canonical autophagy process. Specifically, Atg3, involved in lipidation of Atg8a, and Atg7, involved in both the Atg8 and Atg12 conjugation steps, are not required for RCD in Drosophila midgut ^[63]. Instead, another E1 enzyme in ubiquitination, Uba1, is required in this process ^[62]. Along these lines, Atg5, Atg12, and Atg16, which act together as an E3 enzyme for Atg8a conjugation, are also not required during this process; nor are Atg6 and Atg14, both of which are involved in the nucleation step of autophagy, despite the fact that these proteins are all required for starvation-induced autophagy in Drosophila fat body cells ^[55]. Whether or not the context-specific usage of proteins in autophagy is part of the tight regulation of this process requires further investigation.

The detailed mechanism by which autophagy facilitates ACD remains to be illustrated. Because autophagy flux relies on lysosomal degradation, it is likely that autophagy-dependent cell death also requires the lysosome ^[55][64][65]. The involvement of autophagy in development indicates that the cell has a flexible utilization of molecules involved in this process, but many questions remain regarding the involvement of autophagy in cell death. For example, does autophagy function to degrade major cellular compartments to achieve cell death? Does autophagy promote cell death by degrading survival factors? Is selective autophagy or nonselective autophagy required for ACD?

4. Crosstalk between Autophagy and Regulated Cell Death

The dual role of autophagy in cell survival and cell death makes the question of the interplay between autophagy and RCD more intriguing and important. Recent research has revealed many examples of crosstalk between these two pathways. In mouse hepatocytes that are depleted of Casp1, there is a decrease in the autophagy markers LC3 and BECN1, as well as decreased clearance of mitochondria compared to wild-type cells; overexpressing BECN1 restores the clearance of mitochondria ^[66]. TP53/p53, a major tumor suppressor that can induce cell death, interacts with RB1CC1/FIP200 (a potential human homolog of Atg17) and BECN1 ^[67][68]. These results indicate there is crosstalk between autophagy and RCD.

Further investigation uncovered some of the details of the crosstalk between these two pathways. A number of autophagy proteins are substrates of caspases. For example, stimulation of death receptors, such as FAS and TNFRSF10A/TRAILR1, by death ligands, such as TNF/TNF-α or TNFSF10/TRAIL, leads to a significant increase in ATG3 protein degradation by the initiator CASP8, leading to inhibition of autophagy during extrinsic apoptosis ^[69]. Similarly, initiator caspases CASP9 and CASP10, as well as executioner caspases CASP3 and CASP6, can cleave ATG5, leading to decreased autophagy flux ^[70].

Whereas some of the known autophagy-related caspase substrates experience a loss of function in autophagy upon cleavage by caspases, human ATG4D and BECN1 are proposed to gain pro-apoptotic functions after being cleaved. Among the four human paralogs ATG4A to ATG4D, ATG4D contains a canonical caspase cleavage sequence (DEVD63K) in its N terminus ^[71]. ATG4D is cleaved by CASP3 in vitro ^[71]. Researchers also observed an increased ANXA5/annexin V staining in HeLa cells overexpressing ΔN63 ATG4D, suggesting a role of this ATG4D fragment in apoptosis. Consistent with ATG4D being a caspase substrate in vitro, in human A431 cells and HeLa cells treated with the apoptosis inducer staurosporine, full-length ATG4D is lost, and this can be prevented by co-treatment with the caspase inhibitor Z-VAD-FMK. Interestingly, mutating the catalytic site does not abolish the effect of ΔN63 ATG4D on apoptosis. Further investigation showed that ATG4D can be imported to the mitochondrial matrix, regardless of caspase cleavage. Upon treatment with the mitochondrial uncoupler carbonyl cyanide m-chlorophenyl hydrazone (CCCP), the mitochondrial pool of ATG4D sensitizes cells to cell death ^[72]. Together, these data suggest a role of ATG4D in apoptosis independent of its already known function in autophagy.

BECN1 also demonstrates an interesting balance between its roles in autophagy and apoptosis: (1) BECN1 interacts with BCL2-family proteins via the BH3 domain, positioning itself at the interface between autophagy and apoptosis ^[73]. The association of BECN1 with the BCL2 family inhibits the pro-autophagic role of BECN1 ^[74]. (2) BECN1 is the substrate of multiple caspases, including CASP3, and CASP6 to CASP10 ^[70], and caspase cleavage inhibits BECN1-mediated autophagy. Interestingly, the C-terminal cleavage product of BECN1 can localize at the mitochondria and sensitize the cells to apoptosis, suggesting that caspase cleavage of BECN1 helps coordinate autophagy and apoptosis by inhibiting the former and promoting the latter ^[74][75].

Conversely, regulators of RCD can also be controlled by autophagy or autophagy gene products. Depletion of the Atg5 gene significantly represses apoptosis in mouse embryonic fibroblasts treated with SKI-I (a pan-sphingosine kinase inhibitor), accompanied by suppressed CASP8 activity. Upon SKI-I treatment, CASP8 is recruited to ATG5-positive autophagic membranes, leading to the suggestion that autophagy facilitates the activation of this caspase ^[76]. ATG7 interacts with CASP9 in multiple human tumor cell lines. Overexpressing ATG7 in living cells inhibits the processing of the CASP9 prodomain, suggesting that ATG7-CASP9 interaction sequesters CASP9 and prevents its activation ^[77]; whereas, in a cell-free system, an increased dose of ATG7 inhibits CASP9 protease activity ^[77]. In cells undergoing apoptosis, knockdown of ATG12 inhibits BAX activation and CYCS/cytochrome-c release from mitochondria. Additionally, the antiapoptotic effect of the MCL1 protein can be mitigated by the expression of ATG12, but not the expression of ATG12^V95A, a mutant defective in binding to MCL1 ^[78].

What is the biological relevance of having crosstalk between autophagy and RCD? One of the advantages could be that such crosstalk helps the cell make a definitive “survival” and “death” decision under a given condition and avoid wasting energy. Compared to executing both the pro-survival autophagy and pro-death RCD in response to any stress, it would be more beneficial for the cell to execute only autophagy under mild stress allowing cell survival, and switch to mainly RCD under strong stress conditions, or in response to developmental needs. As mentioned above, the binding of ATG7 to CASP9 weakens the activity of the latter. However, in response to the apoptosis-inducing drug staurosporine, the interaction of ATG7-CASP9 is disrupted, freeing CASP9 from inhibition ^[77]. In this way, cells coordinate the two pathways not only to avoid the waste of energy in having two antagonistic pathways functioning at the same time, but also to focus on one pathway under a given condition. From this perspective of cost and efficiency, perhaps it is not surprising to see that many molecules regulate both autophagy and RCD in a coordinated manner. Another potential advantage of having such a steep gradient of response concerns the context of tissue damage. A steeper switch in the survival or death decision can help limit the damaged area by executing RCD where needed, while allowing cells with less damage to use autophagy to survive. Such a model is proposed for ischemic infarction ^[73].

The explanation above, however, does not explain findings, such as the pro-apoptotic role of ATG12 via binding to BCL2 ^[78]. Thus, the interplay between autophagy and cell death appears to be more complex and intricate. During the course of scientific research, many of the genes involved in the autophagy and RCD pathways were originally named based on the pathways they were first identified within. The pro-death role of autophagy indicates a more complex picture and inspires researchers to view the autophagy process and the genes involved from different perspectives, and the same applies to genes involved in regulated cell death. Consistent with this notion, there are many genes “moonlighting” in both pathways ^[55][70].

The Mitochondrion Is One of the Juncture Points for Autophagy and Regulated Cell Death

As the target of mitophagy, and a platform to initiate apoptosis, the mitochondrion is another convergent point between autophagy and RCD. Mitochondria contain apoptotic factors, such as ENDOG (endonuclease G) and CYCS/cytochrome c. During intrinsic apoptosis, mitochondrial outer-membrane permeabilization (MOMP) is changed, leading to changes in mitochondrial membrane potential and the release of apoptotic factors from mitochondria ^[56]. For example, once released into the cytosol, CYCS/cytochrome c can interact with and cause the oligomerization of APAF1, forming the apoptosome to initiate apoptosis. As mitophagy degrades mitochondrial contents via lysosomal degradation, it could be used to antagonize mitochondria-mediated intrinsic apoptosis, which requires the release of mitochondrial contents, such as CYCS/cytochrome c, into the cytosol. The clearance of damaged mitochondria by mitophagy can therefore increase the threshold for apoptosis initiation. The antagonizing effect of mitophagy and intrinsic apoptosis allows the cells to degrade just one or a few mitochondria when the stress is mild to avoid the execution of apoptosis. For example, the inhibition of mitophagy promotes B5G1-induced apoptosis in drug-resistant cancer cells ^[79]. However, this notion might be too simple to reflect the full relationship between mitophagy and apoptosis, as PINK1 can regulate BCL2-family proteins, and PRKN activation can both facilitate or inhibit cell death ^[80][81]. As mitochondria perform multiple functions from energy and metabolite production to regulating apoptosis, it is not surprising that mitochondrial turnover is under complicated regulation and that there is intricate crosstalk between the pathways involved. The crosstalk between mitophagy and apoptosis has been intensively studied, and the relationship between the two pathways has also been covered by previous reviews ^[81].

This entry is adapted from the peer-reviewed paper 10.3390/biom12070866