The tumor necrosis factor receptor superfamily (TNFRSF) is characterized by the ability to bind tumor necrosis factor superfamily (TNFSF) ligands [25,28]. The TNFRSF comprises 29 receptors, including TNFR1 and TNFR2 and the TNFSF comprises 19 ligands [25,29]. TNFR1 is expressed on almost all cells [30] and has extracellular TNF-binding structures characterized by four repeated cysteine-rich domains (CRDs: CRD1, CRD2, CRD3, and CRD4) [31,32]. TNFR1 exists in its trimetric form before stimulation by TNF-α. Monomeric TNF receptors typically trimerize around TNF ligands that recruit TNF receptor-associated factors (TRAF) to the membrane and initiate a downstream signaling cascade [33,34]. TNFR1 has a cytoplasmic region, designated as the death domain (DD), which initiates a cytotoxic signal [35]. TNFR1 activation of NF-κB is dependent on several molecules such as the receptor-interacting protein kinase 1 (RIPK1, also known as RIP1), TNFR1-associated death domain (TRADD), TRAF2, and Fas-associated death domain (FADD) [36]. TNFR1 activates various responses. For example, TNFR1 promotes inflammatory responses, regulates cytokines, chemokines, adhesion molecules and other receptors, as well as induces the migration of neutrophils, and regulates the complement system [25]. The interaction of TNF-α and TNFRs is significantly involved in the development of lymphatic tissue; TNFR1-deficient mice show reduced numbers of Peyer’s patches and exhibit distressed germinal center formation in the white pulp of the spleen and follicular dendritic cell development and localization. TNFR1 is the likely principal receptor transducing TNF signals during lymphoid tissue development because TNFR2-deficient mice do not show changes in lymphatic organogenesis [37]. Studies have also shown that TNFR1 mediates the immune response necessary to combat infection by microbes such as mycobacteria [38] and Listeria monocytogenes [39], while TNFR2 absence has a negligible effect on supporting this response [40]. In addition, TNFR1 is the major receptor mediating host defense against viral infection [41].

The expression of TNFR2 is restricted compared to the ubiquitous expression of TNFR1. TNFR2 is expressed on endothelial cells, mesenchymal stem cells, and immune cells, such as macrophages, monocytes, T cells, B cells, and NK cells [42,43,44,45,46,47], and neural cells [48]. The extracellular region of TNFR2 contains CRDs (CRD1, CRD2, CRD3, and CRD4) as observed in TNFR1 [31,32], and TNFR2 exists in its trimetric form before stimulation by TNF-α. However, in contrast to TNFR1, TNFR2 lacks a death domain in the intracellular region of the receptor [49]. Although TNFR2 is primarily involved in cell proliferation, activation, and survival, it also induces apoptosis and enhances TNFR1-mediated cell death [50,51]. Upon activation of TNFR2, TRAF1 and TRAF2 interact with the TNFR2 cytoplasmic domain [34]. TNFR2’ signaling through TRAF2 induces JNK activation and uses ASK1 as a mediator to activate NF-κB and facilitate anti-apoptotic signals [52]. Several papers showed that TNFR1, not TNFR2, is the essential receptor for preventing bacterial infections [53,54,55], and TNFR2 has been shown to induce viral resistance in vivo [56]. TNFR1-deficient mice with polymicrobial septic shock attenuate the disease; however, TNFR2-deficient mice with polymicrobial septic shock experience worse symptoms and shorter survival [57]. Together, these studies indicate that TNFR 1 seems to mediate immune responses to bacterial and viral challenges, whereas TNFR2 may be primarily involved in the response to viral infections.

3. Osteoclasts

3.1. TNFR1 and TNFR2 in Osteoclasts and the Role of the Interaction of TNF-α and TNFRs in Osteoclast Formation

RANKL is an essential factor for osteoclast differentiation [58,59] and binds to the membrane-bound receptor RANK on the surface of osteoclast precursors. RANK expression is induced by the macrophage colony-stimulating factor (M-CSF) [60]. While RANKL is a requisite molecule for osteoclast formation, some researchers reported that osteoclast formation still proceeds without RANKL in the presence of TNF-α. For example, osteoclasts form from bone marrow macrophages in vitro independent of RANKL [61,62]. Other researchers argued that TNF-α amplifies the effect of RANKL but does not act alone because TNF-α induces osteoclast formation, given that low concentrations of RANKL were also supplied [63]. To clarify this issue, TNF transgenic mice were crossed with RANK-/- mice, and the lack of RANK resulted in severe osteopetrosis even though TNF-α was over-expressed [64]. Thus, TNF-α mediated osteoclast formation depends on RANKL and is considered to enhance RANKL signaling rather than act alone. Nonetheless, there are particular settings in which TNF-α can induce osteoclast formation independent of RANKL if sufficient cofactors are present. For example, factors such as M-CSF and transforming growth factor-β (TGF-β) act in conjunction with TNF-α to induce osteoclast differentiation in mouse models lacking RANKL, RANK, and TRAF6. Although the formed osteoclasts were not functional, osteoclast functionality was recovered by TRAF6 activation via IL-1 [65]. These findings suggest that TNF-α-induced osteoclast formation acts independent of RANK/RANKL, given that additional cofactors are supplemented and that TRAF6 is essential for osteoclast activation and function. Further studies using different mouse models and culture conditions are necessary to clarify the role of these molecules together and alone.

TNF-α plays a central role in inflammatory osteoclast formation and affects cells involved in osteoclast formation, including macrophages, stromal cells, and T cells expressing RANKL, which enables their osteoclastogenic potential [66,67]. Our previous studies showed that TNF-α induces osteoclast formation and bone resorption in vivo, possibly targeting those cells [68,69,70,71,72,73]. Thus, a better understanding of the contribution of TNF-α in those target cell types may help identify critical therapeutic strategies. A previous study reported that osteoclast precursors are direct targets of TNF-α in vitro [63]. To test the contribution of TNF-α to osteoclast formation, we created four kinds of chimeric mice by manipulating the bone marrow cell content. We transplanted wild-type marrow into lethally irradiated mice lacking TNFRI and TNFRII. The result was mice with stromal cells and macrophages bearing TNFRs, mice with only stromal cells bearing TNFRs, mice with only macrophages bearing TNFRs, and mice with stromal cells and macrophages deficient in TNFRs. Osteoclast formation was evaluated after injection of TNF-α into the supracalvariae of these chimeric mice. The results indicated that stromal cells contribute more to osteoclast formation than macrophages, although both macrophages and stromal cells were found to be direct targets of TNF-α [68,74]. TNF-α acts on macrophages to increase their RANK expression and on stromal cells to increase their expression of RANKL and M-CSF, the latter being essential in inducing RANK expression after monocyte differentiation into osteoclast precursors [68,74].

Several reports showed that the compression force in orthodontic tooth movement (OTM) in mice induces the expression of TNF-α [75,76,77,78,79,80]. Our studies found that TNF-α plays an essential role in OTM in both TNFR1- and TNFR2-deficient mice, which experienced less tooth movement distance compared to wild-type mice [81,82,83,84,85,86]. Furthermore, we showed that chimeras made of TNFRs deficient mice and wild-type mice can be used to examine the type of cell targeted by TNF-α for osteoclast formation during OTM. These findings suggested that the response of stromal cells to TNF-α is a crucial factor for osteoclast formation and bone resorption in OTM [83].

Analysis of bone marrow cells derived from mice differentially expressing TNFR1 or TNFR2 revealed that TNFR1 induced osteoclast formation results are comparable to those obtained in wild-type mice, and marrow from mice expressing only TNFR2 could not stimulate osteoclast formation [87]. Moreover, in the OTM setting, the number of osteoclasts in TNFR1-deficient mice was lower than that in wild-type mice [75]. In contrast, when tooth movement was applied to mice deficient in either TNFR1 or TNFR2, less tooth movement and less osteoclast formation were observed in TNFR2-deficient mice compared with those observed in TNFR1-deficient mice [81]. These results suggested that selective signaling through TNFR1 or TNFR2 has different outcomes and that TNFR1 plays an essential role in TNF-α-induced osteoclast formation.

3.2. TNF-α Signaling Pathways via TNFR1 and TNFR2 for Osteoclast Formation

Understanding TNFR-TNF-α signaling goes hand in hand with understanding the signaling pathways involved in osteoclast differentiation by M-CSF and RANKL. M-CSF is one of the early cytokines involved in the differentiation of monocytes to osteoclasts and the survival of osteoclasts. Osteoclast precursors express the M-CSF receptor c-Fms, and M-CSF, and in turn, activates the mitogen-activated protein kinase (MAPK) pathways, PI3K, and Akt [60].

Differentiation involves the expression of RANK on the surface of osteoclast precursors, which are activated by RANKL, leading to the induction of a plethora of effectors. The downstream signals include MAPK pathways, such as family-mediated JNK, p38, NF-κB, and the AP-1 family, such as c-Fos and TRAF [88]. NF-κB- and c-Fos-deficient mice showed osteopetrosis symptoms, suggesting that NF-κB and c-Fos are essential molecules for osteoclast formation [89,90,91]. TRAF6-deficient mice were used to examine the role of TRAF6 for RANKL-induced osteoclast formation; these mice exhibited reduced numbers of osteoclasts and osteopetrosis symptoms [92,93]. The transcription factors AP-1 and NF-κB promote osteoclast formation and activate the nuclear factor of activated T-cell c1 (NFATc1). NFATc1 is required for osteoclast formation as the master transcription factor in osteoclast formation [94]. NFATc1 is activated by calcineurin-mediated dephosphorylation in osteoclast formation [95]. NFATc1 migrates into the nucleus and binds to promoters of osteoclast-related genes such as TRAP, the calcitonin receptor, and cathepsin K genes [94]. The osteoclast-associated receptor (OSCAR) binds to adaptor molecules associated with immunoreceptor tyrosine-based activation motifs (ITAM) in osteoclast precursors and activates NFATc1 as co-stimulatory signals for RANKL [96].

Previous studies examined whether TRAF6 is required for TNF-α-induced osteoclast formation using osteoclast precursors from TRAF6-deficient mice. The results showed that TNF-α cannot induce osteoclast formation and suggested that TRAF6 was required for TNF-α-induced osteoclast formation in vitro [97]. TRAF6 is not a common adaptor protein for TNFRs. TNF-α synergistically enhances RANKL-induced osteoclast formation but does not act alone, because TNF-α can induce osteoclast formation with RANKL [63]. These studies suggest that TNF-α-induced osteoclast formation might be necessary for the existence of RANKL, and further research is required to explore this possibility. Another study analyzed the role of TRAF2 in TNF-α-induced osteoclast formation using fetal liver cells from TRAF2-deficient mice and found that osteoclast formation induced by TNF-α was severely inhibited. Fewer osteoclasts were formed via RANKL from progenitors from TRAF2-deficient mice than those from wild-type mice [98], indicating that TRAF2 signaling is essential for TNF-α-induced osteoclast formation. The results suggested that TRAF2 signaling promotes TRAF6 signaling in RANKL-induced osteoclast formation. Another study reported that TNF-α-induced osteoclast formation was attenuated in TRAF5-deficient mice in vitro [98]. Further studies are needed to elucidate which TRAF is essential for TNF-α-induced osteoclast formation.

TNF-α signaling induces different biological reactions depending on the receptor activated, either TNFR1 or TNFR2, because each receptor mediates distinct intracellular signals. A study in TNFR1- or TNFR2-deficient mice for TNF-α-induced osteoclast formation in vitro revealed that TNFR1 enhanced osteoclast formation and TNFR2 inhibited osteoclast formation [87]. TNF-α induces activation of MAPKs through phosphorylation of ERK, p38, and JNK in osteoclast precursors [69,85,99]. In addition, TNF-α induces phosphorylation of ATF2 downstream of MAPKs [99] and induces phosphorylation of IκB in osteoclast precursors [85,86,100].

TNF-α has been shown to support osteoclast survival [101] by activating Akt phosphorylation in osteoclasts. TNF-α-induced signaling is inhibited by the PI3K inhibitor LY294002 and the Src family kinase-selective inhibitor, PP1. These inhibitors decreased osteoclast survival. Furthermore, TNF-α-enhanced ERK phosphorylation was observed. In contrast, treatment with PD98059, which is a specific inhibitor of the ERK activating kinase MEK-1, resulted in the inhibition of TNF-α-induced phosphorylation of ERK and the survival of osteoclasts [101]. TNF-α acts mainly via NF-κB activation, leading to the transcription and production of anti-apoptotic factors [102]. TNF-α signaling converges on the mammalian target of rapamycin (mTOR) as part of the anti-apoptotic action of osteoclasts. The TNF-α signaling intermediates for mTOR/ribosomal protein S6K activation include PI3K, ERK, and Akt. Specific inhibitors of these factors inhibited TNF-α-activated S6K and enhanced osteoclast apoptosis. Furthermore, mTOR regulates protein translation through S6K, 4E-BP1, and S6, and the inhibition of these molecules affects protein translation and induces osteoclast apoptosis [103].

TNF-α was indicated to induce actin ring formation, which is a required osteoclast formation in vitro [104]. Previous studies reported that TNF-α regulated forming and a precursor zone for the matured sealing-ring and the nascent sealing zone by L-plastin (LPL) phosphorylation [105,106,107]. TNF-α stimulates the assembly of actin aggregates at the early stage of sealing-ring formation, independent of αvβ3 integrin signaling. TNF-α changed the expression and phosphorylation levels of LPL. In osteoclasts plated on dental bone slices and treated with TNF-α, changes in actin organization on the nascent sealing zone and sealing-ring formation are mutually related with cortactin proteins, and LPL phosphorylation was observed [108]. The MAPK pathway is one of the signaling pathways leading to LPL phosphorylation [109,110]. PKA and PKC induce phosphorylation of LPL in other cell types; however, both PKA and PKC were not observed as downstream of the TNFR1 interacted with TNF-α in osteoclasts [111]. Further studies examined the molecular mechanism of L-plastin phosphorylation and the subsequent formation of the nascent sealing zone in TNF-α-treated osteoclasts. The results showed that the TNF-α signaling pathway via TNFR1 compromised an Src-PI3K-TRAF-6-Rho/Rho-kinase axis for activating the phosphorylation of LPL and regulating nascent sealing zone formation [111].

The signaling pathways activated via TNFR1 and TNFR2 have been intensively studied in a variety of other cell types; few studies have examined TNF-α-signaling in osteoclast precursors/osteoclasts. Further studies are needed to elucidate the relationship between the signaling downstream of the interaction of TNF-α with TNFRs in osteoclast differentiation.

4. Osteoblasts

4.1. TNFR1 and TNFR2 in Osteoblasts

Osteoblasts differentiate from multipotent mesenchymal stem cells (MSCs) capable of producing adipocytes, chondrocytes, fibroblasts, and skeletal muscle cells [112,113,114,115,116]. MSCs commit to the osteoblastic pathway under the transcriptional control of Runx2 (also known as Cbfa1, AML-3, or Pepb2αA) via subnuclear localization and binding to its target DNA foci and the transcription factor osterix (Osx) acting downstream of Runx2 [117,118,119,120,121].

Reports on the expression of TNFRs by osteoblasts have yielded inconsistent results. While studies have agreed on the expression of TNFR1, TNFR2 has been considered by some authors as “not detected” in MG63 osteosarcoma cells and in non-transformed osteoblasts made from mesenchymal stem cells using unamplified gene screening [122]. While TNFR1 is generally expressed among all cell types (except erythrocytes), TNFR2 expression is restricted to immune cells, endothelial cells, and a few other cell types, including MSCs, which express an osteogenic proliferative potential [123,124]. In this review, we continue with the presumption that osteoblasts express both TNFRs because TNFR2-deficient osteoblasts exhibit a phenotype distinct from that of wild-type or TNFR1-deficient osteoblasts [125,126,127]. In the following sections, signaling in osteoblasts through TNFRs is discussed in the inhibition of osteoblast differentiation, apoptosis, and negatively influencing bone remodeling.

4.2. The Role of the Interaction of TNF-α and TNFRs in Osteoblast Differentiation

TNF-α inhibits osteoblast differentiation in cells from multiple cell sources: fetal calvarial cells, the MC3T3 pre-osteoblastic cell line, and murine marrow stromal cells [128,129]. Inhibition occurs in a temporal fashion at a critical time in cell culture (days 2–14 of a 21-day culture) and at very low doses [130]. TNFR-deficient mice exhibit an increased peak of basal bone mass and TNFR1-deficient mice display an increased basal bone mass in different anatomic positions and overall, because of increased bone formation [127].

TNFR1 is the receptor responsible for TNF-α-mediated osteoblast differentiation inhibition, while TNFR2 is dispensable in this process. While wild-type or TNFR2-deficient MSCs cultured with TNF-α exhibit the complete inhibition of osteoblast differentiation, nodular formation, and mineralization as well as decreased alkaline phosphatase activity and osteocalcin expression, TNFR1-deficient MSCs are completely unresponsive to TNF-α and exhibit no effects on osteoblast differentiation. Researchers reasoned that cytotoxicity might be why TNF-α inhibits osteoblast differentiation—because reducing the pool of precursor cells led to a decrease in the number of available osteoblasts. However, MC3T3 apoptotic cell death was not the reason why TNF-α blocks differentiation to osteoblasts, because treatment with caspase inhibitors did not reverse the inhibitory effects of TNF-α [125]. TNFR1 signaling is sufficient to inhibit osteoblast differentiation, which cannot be attributed to increased cell death. TNFR2, although dispensable, confers some protection against the actions of TNF-α [125,130]. TNFR2 did not affect osteoblast differentiation, and it decreased cell sensitivity to TNF-α when cells were treated with murine TNF-α; however, it showed no effect when cells were treated with human TNF-α. This is due to the ability of TNF-α to activate mouse TNFR1 and TNFR2 to similar degrees, whereas human TNF-α activates mouse TNFR1 but does not stimulate mouse TNFR2 [50].

The protective effect of TNFR2 on osteoblast differentiation is puzzling as TNFR2 has been proposed to assist TNFR1 signaling through ‘’ligand passing,’’ in which TNFR2 increases the concentration of TNF-α around TNFR1 due to the kinetic properties of TNF-α association and dissociation from TNFR2 [131]. Conversely, the protective effects of TNFR2 can be explained by the presence of a proteolytic cleavage site encoded by exon 6 in the TNFR2 gene. Cleavage at the extracellular domain of TNFR2 allows it to be released as an active soluble receptor (sTNFR2) that competes with TNFR1, antagonizing TNF-α effects [132]. Alternatively, other studies suggested that, at specific concentrations, TNFR2 may dampen the effect of TNF-α and alter the strength of signaling through TNFR1 in the same cell [133]. TNFR2 is strongly activated by mTNF-α, leading to cellular death, but is resistant to activation by sTNF-α; this observation, in addition to the kinetics of receptor binding, may explain the different response of osteoblasts harboring one of either receptor [134].

TNF-α inhibits osteoblast differentiation distal to the signaling of known osteogenic inductive proteins (IGF-I, BMP-2, BMP-6, and LMP-1) and by directly affecting Runx2 activity at multiple levels. First, TNF-α regulates Runx2 at the transcriptional level by decreasing mRNA expression of the two major Runx2 isoforms (P1/MASNS to 50%, P2/MRIPV to 90%), and second, by reducing mRNA half-life and stability, which translates into a reduction in the Runx2 nuclear content as well as a reduction in Runx2 binding to promoter regions [128,130]. Interestingly, TNF-α antiproliferative and apoptotic effects on osteoblasts may be facilitated by Runx2 itself; hMSCs transfected with siRNA designed to inhibit Runx2 expression are less sensitive than hMSCs transfected with a nonspecific siRNA. This may explain the conflicting results TNF-α has on osteoblast apoptosis (see below) as TNF-α effects on osteoblasts require active Runx2 expression [129].

Various post-transcriptional and post-translational mechanisms regulate Runx2 expression and activity. BMP-2 regulates Runx2 via stabilizing its binding to Smads and preventing its degradation by Smurf1/2 [135,136]. TNF-α upregulates Smurf1/2 activity, leading to degradation of Runx2. Furthermore, Smurf1-/- mice are rescued from TNF-α degradation of Runx2 [137,138]. TNF-α directly reduces alkaline phosphatase activity and reduces BMP-2/BMP-4-induced alkaline phosphatase activity when added to MC3T3 cells. Inhibiting osteoinductive signaling molecules is another mechanism in which TNF-α negatively affects osteoblasts without inhibiting Runx2 directly in a mechanism that involves NF-κB inhibition of TGF-β and Smad signaling [127,139]. BMP-2 and BMP-4, in turn, exert inhibiting effects on TNF-α by blocking TNF-α-mediated apoptosis of pluripotent mesenchymal C2C12 cells via preventing the caspase 8–mediated cleavage of Bid [140]. BMPs suppress the signal initiated by TNF-α binding to TNFR1 through stabilizing Smad signaling [140,141,142].

In line with the established role of TNF-α in inhibiting osteoblast differentiation, TNF-α also decreases Osx mRNA expression and Osx promoter activity in M3CT3 cells; however, it does not affect the brief half-life of Osx mRNA as it does with Runx2 mRNA. TNF-α inhibition of Osx promoter activity and mRNA expression is due to the activation of the MEK/ERK pathway, while p38 and JNK are not involved in this process [15]. NF-κB activation by TNF-α positively influenced Osx promoter activity in MC3T3 cells; the blockage of NF-κB did not affect TNF-α action, and overexpression of NF-κB concomitant with TNF-α treatment did not inhibit Osx transcription. This is due to the binding of NF-κB to a low-affinity enhancer element of the Osx promoter that cannot be sufficiently stimulated by the low levels of NF-κB activated by TNF-α. One study demonstrated that the regulation of NF-κB activity helps balance the effect of TNF-α in cases of severe inflammation, dampening the catabolic effects of TNF-α on bone [15]. A study in another cell model, C2C12, showed that NF-κB activation by TNF-α conferred protection against osteoblast apoptosis [140].

TNFR1 and TNFR2 activate the NF-κB pathway using TRADD and the TRAF1/TRAF2 complex. Furthermore, signaling through TRAF1/2 to activate NF-κB promotes cell survival, for the most part, while the case is not the same for TNFR1 signaling through TRADD, which promotes survival or apoptosis [133]. Which of the two receptors regulates Osx activity needs further study.

After reducing Runx2 and osterix mRNA expressions, TNF-α suppresses alkaline phosphatase and α1(I) procollagen gene expressions. The inhibition of skeletal-specific matrix proteins, osteonectin, and osteopontin is only seen with TNF-α but not with other inhibitory cytokines, such as IL-1β, which are also reported to inhibit osteogenic differentiation [114].

4.3. The Role of the Interaction of TNF-α and TNFRs in Osteoblast Apoptosis

TNF-α apoptotic effects on osteoblasts depend on the cell differentiation stage. While cells expressing low levels of Runx2 show inhibition of differentiation, mature osteoblasts expressing high Runx2 levels are susceptible to apoptosis by TNF-α signaling through TNFR1 [129]. TNF-α induces apoptosis in MC3T3 osteoblastic cells, an effect amplified by serum starvation [143], and induces DNA fragmentation at 20 ng/mL, as observed by BrdU-labeled DNA staining after 24 h [144]. TNF-α also reduces proliferation and induces early and late apoptosis of MSCs in a dose-dependent manner [145,146]. Other studies reported contradicting results; TNF-α had no effect on MSC viability or apoptosis, but it did decrease MC3T3 viability only after complete differentiation of osteoblasts occurred; it also increased caspase 3 activity slightly but significantly [125,130]. In another report, TNF-α treatment did not increase apoptosis or proliferation of primary human osteoblasts or the MG63 osteoblastic cell line; however, pretreatment of cells with TNF-α increased Fas expression on the cell surface, and the incubation of cells with anti-Fas IgM significantly increased Fas-mediated apoptosis of these cells [147]. Differences in osteoblast susceptibility to induction of apoptosis may be influenced by the cell differentiation stage and culture conditions in the studies, with cells in polygonal nodular osteoblast-assuming shape being more susceptible to apoptotic stimuli than less differentiated cells [122].

Transient caspase activation, in particular, caspases 2, 3, and 8 by BMP-4 is important for osteoblast differentiation, and the inhibition of these caspases leads to reduced ALP and PTH activity in MC3T3 cells. Paradoxically, triggering the caspase cascade signal is important for apoptosis; however, the critical difference in whether apoptosis or differentiation occurs is in the level of differentiation of the cells when treated with TNF-α. These results reconcile the finding that caspase activation is essential for osteoblast differentiation with the lack of apoptosis that is seen in not fully differentiated cells. Cells that have already assumed the osteoblastic phenotype are susceptible to TNF-α-induced caspase activation and apoptosis. In contrast, cells that have not yet differentiated and that are still pre-osteoblastic require the activation of the caspase pathway to assume the mature osteoblastic phenotype [144].

4.4. The Role of the Interaction of TNF-α and TNFRs in Osteoblast for Bone Remodeling

TNFR activation in osteoblasts generally inhibits bone formation and stimulatory bone resorption. Downstream activation of TNFRs inhibits transcription and translation of genes favoring bone formation, including α-collagen, alkaline phosphatase, osteocalcin, insulin-like growth factor 1, parathyroid hormone receptor, platelet-derived growth factor receptor, and N-cadherin genes. TNF-α induces transcriptional activation of genes in osteoblasts favoring indirect bone resorption, such as IL-6, tPA, uPA, PAI-1, gelatinases, MMPs, TIMPs, NF-κB, ICAM-1, CSF-1, GM-CSF, and G-CSF genes [130,139,148,149,150,151,152,153,154,155,156,157,158,159]. These effects on osteoblasts are elicited by activating TNFRs through TNF-α; notably, these effects were not tested on a receptor type basis, but we can only speculate through studying their downstream effectors.

The intracellular domains of TNFR1 and TNFR2 share no homology; however, TNFR1 and TNFR2 activate nearly the same pathways via different mechanisms. Upon activation, both receptors are inert and signal through TRAFs. The main difference between the two receptors is the presence of a DD only in TNFR1. The DD associates with the silencer of the death domain (SODD); upon activation of TNFR1, SODD frees the cytoplasmic domain to associate with the TRADD protein, which then recruits TRAFs to propagate the downstream signal of the receptor. The main pathways activated by TNFRs are the NF-κB, MAPK family, G protein family, and caspase pathways. Some differences are present in the selectivity and efficiency of either receptor to activate these pathways; however, their functions overlap considerably. The downstream activation of both receptors is required for the full effects seen in osteoblasts compared with effects observed upon activation of just one of the two receptors [160,161].

While TNF-α is generally inhibitory of bone formation, TNF-α shows a dual function in fracture healing. In the early stages of fracture healing, a brief but necessary inflammatory stimulus recruits osteoblast progenitors cells capable of forming osteoblasts. This observation was demonstrated in two models of fracture repair—marrow ablation and simple transverse fractures—in which TNFR-deficient mice experienced delayed healing. On day three after model establishment in wild-type mice, young osteoblasts appeared in the marrow space, while only granulation tissue appeared in the marrow cavity in TNFR-deficient mice. In the marrow ablation model in TNFR-deficient mice, type I collagen and osteocalcin mRNA expressions were reduced to 50% of levels in wild-type mice. There was a complete absence of the initial intramembranous bone formation on the periosteal surfaces in the fracture repair model. In TNFR-deficient mice, endochondral bone formation was delayed but the inhibition of osteogenesis was not observed; the intramembranous bone formation was initially completely inhibited [162].

In bone resorption induction, TNF-α is a RANKL synergist, and in bone marrow stromal cells, it induces RANKL expression through activating p38 MAPK; inhibiting p38 signaling arrests its expression. TNF-α also induces other MAPK family members, including JNK and ERK [163]. TNF-α induces IL-1 expression in osteoblasts. IL-1 acts by activating MAPKs and NF-κB, eliciting RANKL expression and promoting osteoclast formation [99,164]. TNF-α-induced RANKL and IL-1 expressions are attenuated by IL-4 via suppressing p38 activation, which inhibits osteoclast formation and bone destruction in vitro and in vivo [99, 69].

The effects of TNF-α on osteoblasts obtained from different cell sources converge towards a common outcome. Fetal calvarial cells and murine marrow stromal cells differentiation to osteoblasts is halted when cultured with TNF-α. Additionally, MC3T3 cell line treatment with TNF-α exhibits an inhibition of differentiation via decreasing Runx2 and Osx mRNA expressions and increased apoptotic changes. MSCs, in addition to experiencing inhibition of differentiation, experience reduced nodular formation and mineralization, decreased alkaline phosphatase and osteocalcin expression when signaling through TNFR1. They also experience apoptotic changes once their differentiation to the osteoblastic phenotype takes place, as MSCs apoptosis was shown to be facilitated by Runx2 expression. Cells from the C2C12 line experience apoptosis with TNF-α treatment which can be overcome with BMP-2 and BMP-4, and activation of the NF-κB pathway. Primary human osteoblasts and the MG63 osteoblastic cell line also experience apoptotic changes when treated with TNF-α due to increased Fas expression.