Brassinosteroids in Growth and Development of Plants: History
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Subjects: Agriculture, Dairy & Animal Science
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Hakim Manghwar

Brassinosteroids (BRs) are a group of polyhydroxylated steroidal phytohormones that are required for the development, growth, and productivity of plants. These hormones are involved in regulating the division, elongation, and differentiation of numerous cell types throughout the entire plant life cycle.

  • brassinosteroids (BRs)
  • plant
  • biotic stress
  • abiotic stress

1. Introduction

Plants are exposed to a wide range of biotic and abiotic stresses throughout their life cycle and need to constantly regulate their physiological and developmental processes for responding to numerous internal and external stimuli [1]. Various biotic and abiotic stresses significantly contribute to major global crop production losses by primarily influencing the stress tolerance/adaptive ability of plants [2]. Plants utilize various signaling molecules, including hormones for mediating the plant response to the number of stresses [3][4]. Phytohormones have been widely considered as the natural activators for plant growth and development. They maintain healthy life in plants, and play an essential role in defense mechanisms against various stresses [5]. Phytohormones initiate a signaling cascade that involves a number of molecular players, which lead to an ideal generic pathway [6]. Brassinosteroids (BRs) are an important group of plant hormones involved in regulating plant growth and development, and they help plants to adapt to the environment [7]. Biosynthesis and signaling of the hormone have been extensively studied since its discovery, particularly in the Arabidopsis (Arabidopsis thaliana), which led to a comprehensive understanding of BR synthesis and its signaling pathways [8][9].

2. Discovery of BRs in Different Plant Species

BRs were initially discovered in Brassica napus pollen on the basis of their ability to promote growth [10]. BRs have been discovered as stimulants for plant cell elongation and division. BRs were subsequently named ‘brassins’. Brassinolide (BL), the most active BR, was isolated in 1979 [11]. The most significant finding was the isolation of Brassinosteroid insensitive 1 (BRI1)—a receptor kinase that triggers an intracellular signaling cascade in response to extracellular BR perception [12]. Since the discovery of BL, a huge number of chemically different BRs have been discovered throughout the plant kingdom, including green algae and land plants, suggesting that BRs evolved early during plant evolution. BRs were identified as plant hormones after discovering BR-deficient mutants in A. thaliana [13]. Among all BRs studied to date, Castasterone (CS), Typhasterol (TY), Brassinolide (BL), 6-deoxocastasterone (6-deoxoCS), 28-norcastasterone (28-norCS), and Teasterone (TE) are commonly present in various plant species throughout different environments [14][15][16].

3. Occurrence of BRs in Plants

BRs have been found in organs and all parts of the plants, such as leaves, stems, roots, flowers, pollen, anthers, and seeds [17][18]. BR is universally distributed in all growing tissues of higher plants, but significantly higher concentrations have been detected in seed, pollen, and fruit [19]. The level of BR in the young tissues (1–100 ng/g fresh weight) is normally higher than in mature ones (0.01–0.1 ng/g fresh weight) [20]. BL and CS are the most significant BRs because of their higher biological activity and widespread distribution in plants. However, due to its commercial availability, 24-epibrassinolide (EBR) is the most commonly used BR for studying the physiological effects of exogenous steroid phytohormones on plants [21].
After the BL discovery, about 69 BRs have been identified in 64 plant species, including 6 gymnosperms, 53 angiosperms (41 dicotyledons and 12 monocotyledons), 1 pteridophyte (Equisetum arvense), 3 algae (Hydrodictyon reticulatum, Cystoseira myrica, and Chlorella Vulgaris) and 1 bryophyte (Marchantia polymorpha) [17][18], a moss (Physcomitrella patens), lycophytes (Selaginella moellendorffii and S. uncinata), and 13 fern species [22]. The concentration of 6-deoxotyphasterol (6-deoxoTY) concentration was found to be 6400-fold greater than BL in the pollen of Cupressus arizonica. Additionally, the highest concentration of BR, 6.4 mg 6-deoxoTY per kilogram (kg) pollen, has been found in C. arizonica [17]. Only 52 BRs have been reported in terms of their biological activities in plants [16]. The CS, BL, TY, 6-deoxoCS, TE, and 28-norCS are the most abundant BRs in plants [14][15][20]. The most extensive variety of BRs (2 conjugated and 25 free forms) was found in unripe bean (Phaseolus vulgaris) seeds [20]. Other legumes having fewer BR members have been found in shoots, seeds, and pollen, and their quantity was between 0.007 and 628l g/g fresh weight [14].

4. Chemical Structure of BRs

BRs have been categorized into three major types on the basis of each steroid molecule’s carbon number (i.e., C27, C28, and C29) [23]. The 5α-cholestane skeleton is the basic structure of C27-BRs, 5α-ergostane for C28-BRs, while 5α-stigmastane is the basic structure of C29-BRs. The structure of these hormones differs because of the type and orientation of oxygenated functions of A and B rings, and the number and position of functional groups in the side chain of the molecule. These changes occur as the result of oxidation and reduction reactions during biosynthesis. In general, BRs have been classified into conjugated (5) free, and (64) compounds [23].
BRs have vicinal hydroxyl groups in relation to the A-ring at C-2α and C-3α. BRs with α and β-hydroxyls, or ketone at the C-3 position are the progenitors of the BRs with 2α, 3α-vicinal hydroxyls. BR containing 2α, 3β-, 2β, 3α-, or 2β, 3β-vicinal hydroxyls maybe the precursors of 2α, 3α-vicinal hydroxyls. Two 2α, 3α-vicinal hydroxyl groups on the A-ring are responsible for overall structural attributes of the most active BRs, such as BL and CS. The declining order of activity 2α, 3α > 2α, 3β > 2β, 3α > 2β, 3β suggests that the α-oriented hydroxyl group at C-2 is essential for biological activity of BRs in plants [24][25]. On the basis of cholestane side chain, BRs have been divided by different substituents into C-23, C-24, and C-25: 23-oxo (4 compounds), 24-methylene (3 compounds), 24S-ethyl (4 compounds), 24R-methyl (5 compounds), 24S-methyl (23 compounds), C-25, 24-ethylidene (3 compounds), 24-methyl-25-methyl (3 compounds), 24-methylene-25-methyl (6 compounds) without substituent at C-23 (3 compounds), without substituent at C-24 (8 compounds), and without substituents at C-23, C-24 (2 compounds) [26][27][28].
BRs are classified into 6-oxo (6-ketone) (34 compounds), and 6-deoxo (non-oxidized) (21 compounds), and 7-oxalactone (12 compounds) types depending on B-ring oxidation stage. However, only one fourth type BR with hydroxyl group at C-6, such as 6α-hydroxycastasterone (6α-OH-CS) has been observed. In contrast, two compounds, for instance, 28-nor-(22S)-22-hydroxycampestrol (28-nor-22-OHCR) and (22S)-22-hydroxycampestrol (22-OHCR) were identified as a fifth type of BRs. Generally, 7-oxalactone BRs have been observed to show stronger biological activity compared to 6-deoxo type, and 6-oxo type. Sometimes 6-oxo BRs show activity similar to 7-oxalactone compounds, while non-oxidized BRs exhibit essentially little activity in the bean internode test or very little in the rice lamina inclination test [29][30][31].

5. Role of BRs in Growth and Development of Plants

BRs are steroid hormones that play various roles in the growth and development of plants [32][33][34] (Figure 1). BRs regulate various developmental and physiological processes in plants, such as expansion, cell division, stem cell maintenance, vascular development, elongation of different cell types, and floral transition [35][36][37][38]. Moreover, they play diverse roles in hypocotyl elongation [39], root growth [7][40], shoot growth [28], stomata patterning [41][42], pollen tube growth, seed germination, and pollen germination and development [43], treachery element differentiation [44], xylem formation [45][46], xylem differentiation, photomorphogenesis and plant reproduction [47][48], and senescence [49]. BRs have the ability to activate the cell cycle during seed germination [50], regulate cell cycle progression [51], control leaf cells’ proliferation [52], and induce excessive growth in hydroponically grown plants [53]. BRs also regulate the abiotic and biotic stress responses and stomata development [7][34][54]. Moreover, BR plays a vital role in regulating male and female fertility in crops [55][56]. BR plays a role in etiolation and promotes the elongation of stigma [19], plant architecture, thermo-tolerance, proton transport, tiller number, leaf angle, and leaf size [57][58]. In addition, the exogenous application of BR or alteration in their biosynthesis and signaling could improve crop yields [59][60].
/media/item_content/202201/61f35e0b9d98eijms-23-01012-g001.png
Figure 1. Role of Brassinosteroids (BRs) in growth and development of plants.
BRs are also involved in the regulation of several genes in plants (Table 1). In a study, Chen et al. [61] found that BRs induced WRKY46, WRKY54, and WRKY70 genes that were observed to play positive roles in BR-regulated plant growth in A. thaliana. In another study, the histone lysine methyltransferase SDG8 is involved in BR-regulated gene expression. The knockout mutant sdg8 displayed a reduced growth phenotype with compromised BR responses in A. thaliana [62]. In A. thaliana, BR regulates the seed development and affects the seed size/weight and number by transcriptionally modulating the genes and pathways that regulate the development of the seed and ovule [63][64]. Furthermore, BRs regulate root Nitrogen foraging response in A. thaliana during mild Nitrogen deficiency. A Brassinosteroid signaling kinase 3 (BSK3) gene is involved in the elongation of primary root during mild Nitrogen deficiency [65].
BR regulates the grain yield and plant architecture in rice [23]. The gene products of BRD1 and D11 are involved in the biosynthesis of BR, and affect the height of rice plants [66]. The OsDwarf2/OsDwarf1 reduction encodes a C-6 oxidase needed for BR biosynthesis in rice, reducing second internode and seed length elongation [66][67]. In rice, BRs mediate the effects of N fertilization on spikelet development and contribute to promote spikelet growth by increasing the level of antioxidant system (AOS) and energy charge during panicle development [68]. In maize, inadequate BR biosynthesis causes male sterility due to failure of pollen and anther growth [55][56]. In cotton, both the fiber initiation and elongation of cultured cotton ovules have been reported to require BRs [69][70]. Moreover, an exogenous application of EBR delays the vegetative to generative transition in monocotyledonous wheat (Triticum aestivum L.). Brassinazole, a BR inhibitor, accelerates the transition and heading stage [71]. The application of BR accelerated winter rapeseed maturation by 4 to 8 days [72].
Priming of lucerne seeds with BL considerably increased length, vigor, and fresh and dry shoot and root weights [73]. The application of BR promoted the ripening of grape berry [74]. Treatment with EBR was observed to substantially increase sucrose synthase (sucrolytic) and soluble sugars content in berries [75]. Treatment with BR helped in reducing the decay of jujube fruits, likely due to its ability to postpone senescence and induce disease resistance [76]. Additionally, in potato tubers, the BRs have been shown to promote apical meristem growth [77], accelerating the cell division rate in isolated protoplasts of Petunia hybrida [78]. In Pharbitis nil, CS and BL treatments inhibit flowering in combination with the inductive photoperiod, implying that BR works in tandem with environmental cues to ensure the proper reproductive transition [19][79].
BRs can participate in physiological processes in response to stress by tuning plant growth, and improving plant performance by interacting with plant growth regulators or other plant hormones [80][81]. The disruption of BR signaling affects several developmental processes, including seed development [64], pollen development [82], and flowering time [83]. Plants with BR deficiency are dwarfed [84], and exhibit altered stomatal development [41][85], reduced male fertility, shortened hypocotyls, petioles and internodes, downward curled leaves, and delayed flowering [82]. Moreover, BR-deficient plants also have a compact plant structure because of the reduced lamina inclination. However, BR deficiency can reduce grain size, seed fertility and tiller number [9], improper stomatal distribution, and reduce seed germination [86]. BR-insensitive and -deficient mutants are often referred to as late flowering due to their retarded growth [87][88]. In contrast, plants over accumulating BRs display elongation of petioles and hypocotyls and increased height [89].
Table 1. Involvement of various Brassinosteroid--regulated genes in plant growth and development.

Gene

Description of Gene

Crop/Plant

Role in Growth

Reference

CESA

The CESA gene superfamily, encoding the catalytic subunits of cellulose synthase

Arabidopsis

(A. thaliana)

Plays a role in regulating the cellulose synthesis

[90]

CYCD3;1

Cell division markers

Arabidopsis

(A. thaliana)

Needed for normal cell cycle progression

[51]

Histone lysine methyltransferase SDG8

In Arabidopsis, there are 43 SET Domain Groups (SDG), which contain proteins with conserved SET domains

Arabidopsis

(A. thaliana)

Involved in BR-regulated gene expression

[62]

WRKY46, WRKY54,

and WRKY70

The WRKY family TFs are composed of over 70 members in Arabidopsis

Arabidopsis

(A. thaliana)

Play positive roles in

BR-regulated plant growth and drought stress

[61]

Brassinazole-resistant 1 (BZR1), and BES1-interacting MYC-like proteins (BIMs)

BZR1; BR-activated transcription factor (TF) and BIMs; bHLH TF

Arabidopsis

(A. thaliana)

BR signaling promotes vegetative growth by inhibiting the floral transition

[91]

Transcripts of autophagy-related genes (ATGs)

Autophagy-related genes

Tomato

(Solanum lycopersicum)

Enhanced level of BR triggers ATGs and formation of autophagosomes

[92]

VvHMGR

Plays a role in the mevalonate (MVA) pathway

Grape berries

(Vitis vinifera)

Involved in increasing

the anthocyanin content and promoting coloration. Accumulates the fruit sugar components, and decreases the tartaric acid content

[93]

This entry is adapted from the peer-reviewed paper 10.3390/ijms23031012

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