3D-Bioprinting for Chronic Wound: History
View Latest Version
Please note this is an old version of this entry, which may differ significantly from the current revision.
Subjects: Dermatology | Medicine, Research & Experimental
Contributor:
Syafira Masri

Skin substitutes can provide a temporary or permanent treatment option for chronic wounds. The selection of skin substitutes depends on several factors, including the type of wound and its severity. Full-thickness skin grafts (SGs) require a well-vascularised bed and sometimes will lead to contraction and scarring formation. Besides, donor sites for full-thickness skin grafts are very limited if the wound area is big, and it has been proven to have the lowest survival rate compared to thick- and thin-split thickness. Tissue engineering technology has introduced new advanced strategies since the last decades to fabricate the composite scaffold via the 3D-bioprinting approach as a tissue replacement strategy. Considering the current global donor shortage for autologous split-thickness skin graft (ASSG), skin 3D-bioprinting has emerged as a potential alternative to replace the ASSG treatment. The three-dimensional (3D)-bioprinting technique yields scaffold fabrication with the combination of biomaterials and cells to form bioinks. 

  • 3D-bioprinting
  • cellular activity
  • precision medicine
  • bioinks
  • wound healing
  • biomaterials

1. Introduction

The skin substitution approach has been extensively accepted for clinical use to enhance wound closure and promote normal skin function [1]. Dry wound dressing, including gauze and bandages, are widely used in the early stage of wound healing [2]. The goal of wound dressings is to promote wound closure, enhance new tissue formation, and reduce scar formation. Clinically, the autologous split-thickness skin graft (ASSG) remains a gold standard for extensive wound treatments. It involves taking a specific thickness of healthy skin from other patients and reapplying the ASSG onto the injury site [1]. However, patients with severe burns may not receive adequate skin grafts and are at a greater risk of acquiring infections, including hepatitis B or C [3]. Besides, another traditional approach for chronic wound therapy is via fish skin acellular treatment [4]. This method is considered as one of the significant treatments due to its histological properties that promote cellular regulation and is rich with omega-3 fatty acids to supply to the local tissue [5][6].

1.1. Wound Healing

Wound healing is a dynamic and complex process that initiates the immune response for tissue repair [5]. Several types of wounds, including vascular ulcers, pressure ulcers, and diabetic ulcers, are primarily categorized as chronic wounds [6]. The abnormal pathological conditions of chronic wounds lead to a poor healing rate or excessive scar formation after recovery. Generally, the chronic wound is the most critical challenge related to skin problems. The wound healing phases start immediately after wound formation, followed by the inflammatory phase begins after the hemostasis phase is completed [7]. The hemostasis phase involves the activation of the enzyme precursors, which results in platelet aggregation at the wound site. Thus, the production of a fibrin clot (fibronectin and factor XIII) will be activated to prevent excessive blood loss [8][9]. Besides, the secretion of extracellular proteins, including plasma fibrinogen and fibronectin, promotes wound closure by accelerating cell migration, proliferation, and function [10]. Overlapping the hemostasis process, the inflammation phase helps to recruit the inflammatory cells to the wound area. In this cascade, the inflammatory cells will eliminate pathogens from the wound site and prevent severe complications. Within two to ten days of post-injury, the proliferation phase will take place, where new tissue formation begins with cell proliferation and migration of keratinocytes towards the lesion [11]. Finally, the tissue remodeling begins after several weeks of the injurious event and may last over more than a year [11]. During this phase, all of the essential cellular responses that were stimulated during injury are downregulated and eventually terminated [8]. Figure 1 shows the graphical abstract for wound healing phases, as discussed in the review paper of A.Przekora (2020) [12].
Ijms 23 00476 g001 550
Figure 1. Graphical abstract for wound healing phases [12]. Used under the Creative Commons License (http://creativecommons.org/licenses/by/4.0/) accessed on 13 November 2021.
Chronic wounds are more likely to occur by sustained stimulation, such as hyperglycemia, chronic inflammatory responses, or persistent tissue injury [13]. Non-healing wounds fail to complete the entire wound healing stages and usually have prolonged inflammatory phases. Interruption of the normal healing phase may result in additional phases of a chronic condition, which may indirectly increase the patient’s vulnerability to infection and, ultimately, damage the patient’s quality of life [14]. Problematic wound healing can occur due to a wide range of health conditions and pathologic developments, including chronic inflammation, persistent infections, “open wounds”, and cancerous wound transformation [15]. Diabetes mellitus (DM) has a serious complication that might result in diabetic foot ulcers (DFU). DFU has been related to poor wound healing progress due to cytokines and poor cellular responses, infections, poor vascularisation, and diebetic neuropathies [16]. The primary goal of wound healing is to prevent the wound from being infected by the pathogens from the external environment [17]. Thus, the neutrophil influx is an early inflammatory response required for the clearing of pathogens and cellular debris during cutaneous wounds [18]. Hence, faster wound repair is vital for wound healing treatment. Figure 2 shows the comparison of normal and chronic wound conditions.
Ijms 23 00476 g002 550
Figure 2. A comparison between normal and chronic wounds.
Tissue engineering has proposed a combination of cells, biomolecules, and biomaterials approach to replace the conventional skin graft. The complex structure of skin tissue requires a combination of several types of elements to form a biocompatible scaffold that mimics the native tissue. Thus, three-dimensional (3D) bioprinting is an innovative fabrication technique that combines selected cells with “inks” composed of biomaterials, crosslinkers, and growth factors to fabricate tissue-like structures for various applications. On the other hand, the use of 3D-bioprinted technology decreases the number of operations necessary for skin replacement. The 3D-shaped bioscaffolds open up new alternatives, such as broadening the range of structures accessible to treat injured skin tissues [19]. It allows for the precise placement of skin cells to replace damaged skin [20]. The bioscaffold has the potential to generate better properties for skin constructs with good elasticity, extensibility, and a high yield of skin reconstruction [1]. The network of blood vessels may be printed as well to ensure the long-term survivability of the skin tissue.

1.2. Current Trend of 3D-Bioprinting for Chronic Wound

Although skin has a highly complex structure, bioprinting techniques are the most reliable and convenient transfer of cells with accurate printing outputs and mimic native skin tissue [21]. In skin tissue engineering, 3D-bioprinting is continuously changing as researchers innovate and propel the field ahead. The recent trend in using the 3D-bioprinting approach for chronic wound healing treatment is still under study with several limitations. Figure 3 shows the current trend of the publications for chronic wound healing treatment by using a 3D-bioprinting approach from the year 2010 until 2020. A comprehensive search strategy was followed to collect the digital publication records on Web of Science. The search was limited to articles published from the year 2010 until 2020. The search query consists of seven terms including “3D-bioprinting”, “bioinks”, “three-dimensional”, “tissue engineering”, “skin cells”, “skin regeneration”, and “wound healing”. The publication summary (Figure 3) indicates that the research for chronic wound healing treatment by using 3D-bioprinting was highest in the years 2018 and 2019 compared to the previous eight years. The researchers used different types of biomaterials as bioinks. However, most of the biomaterial entails certain limitations, and the bioinks used successfully met the skin cells’ ideal conditions, including dermal fibroblasts (DFs) and keratinocytes (KCs).
Ijms 23 00476 g003 550
Figure 3. The current trend of SCI-indexed publications on Web of Science for chronic wound healing treatment by using a 3D-bioprinting approach.

2. Human Skin Structure

Skin is the largest organ of the human body with three different complex layers (epidermis, dermis, and hypodermis) and several other components, including the extracellular matrix (ECM), blood capillaries (veins and arteries), nerves, and hair follicles [12]. It is essential for maintaining skin integrity and stability for appropriate function in retaining body homeostasis [22]. Figure 4 shows the illustration of the complexity of human skin structure.
Ijms 23 00476 g004 550
Figure 4. Complex human skin structure (epidermis, dermis, and hypodermis).
The epidermis layer is abundant with keratinocytes to protect the skin from external infections, whereas the dermis layer acts as the skin’s appendages [23]. The dermis is made up of fewer cellular constituents, primarily fibroblasts [24]. The dermis layer lies within a complex connective tissue structure occupied with nerves, hair follicles, glands, and blood vessels for nutrient transportation [21]. Dermal fibroblasts (DFs) are the most abundant cells that occupy the dermis layer of the skin [25]. The dermis is composed of two connective tissues that interact to form an interconnected network of collagenous and elastin fibers produced by DFs [26]. The well-vascularisation inside the dermal layer will supply nutrients to the DFs. In the skin, DFs are responsible for the secretion of growth factors and extracellular matrix (ECM) for tissue regeneration [27]. The subcutaneous tissue, or hypodermis, is a fibrofatty layer that is loosely connected to the dermis layer of the skin [28]. The hypodermis is mainly composed of adipose tissue, which serves as an energy storage and insulation system for the body as well as a cushion for the skin. A muscle layer can be found adjacent to this layer, which overlies either bony prominences or interior tissues and organs. It is also the site of the formation of certain blood vessels that extend into the dermis [29].

3. 3D-Bioprinting for Chronic Wound

Nowadays, in parallel with the advance of technology, direct printing of living cells and biomaterials have opened up new possibilities for 3D tissue engineering and regenerative medicine [30]. The final product for the 3D-bioscaffolds is in the form of a hydrogel. Hydrogels are widely perceived as one of the excellent wound dressings [31]. The selection of bioinks must meet certain criteria, including printing resolution, gelation, viscoelasticity, mechanical properties, and biocompatibility to maintain the viability of the cells upon bioprinting [32]. The interaction of cells with the components of the bioinks needs to be considered for developing a harmoniously organized tissue [33]. Previously, the generation of autologous single-layer keratinocytes, single layer fibroblasts, and bilayer skin in prior work (MyDermTM) was successfully implanted in patients [34]. The success of this work has proven that tissue replacement can be accomplished by using the patient’s cell with a combination of autologous biomaterial. Besides, this approach also eliminated the risks of immune rejection upon post-implantation failure. Consequently, it is preferable to use a biomaterial that maintains a homogenous solution of encapsulated cells with minimal cell sedimentation [31].

3.1. In Vitro Skin 3D-Bioprinting

In vitro skin bioprinting aims to improve the tissue maturation progress before transplantation to the wound site is performed [35]. As a result, this approach allows rapid wound healing progress and tissue regeneration. The usage of appropriate bioinks allows the composite scaffold to achieve adequate pore sizes, improve mechanical strength, and optimize the biodegradation rate for future clinical applications [36]. The bioinks optimization step is designed to provide a cell-friendly environment that promotes cell proliferation rate. However, the most challenging aspect of skin bioprinting is to combine various types of cells in the bioinks for skin tissue reconstruction. Dermal fibroblasts (DFs) and keratinocytes (KCs) are the major cells involved in skin model development [37]. Figure 5 shows the in vitro 3D-bioprinting of the skin layer by using DFs and KCs at different layers.
Ijms 23 00476 g005 550
Figure 5. In vitro 3D-bioprinting using extrusion-based bioprinting.
The 3D microenvironment is required to facilitate cell development and maturation. The DFs and KCs easily isolated from any healthy skin biopsies samples using the standard operative procedure. Skin tissue promotes oxygen transportations and nutrients to all surrounding tissue; it is critical for developing a new tissue/organ with a vascularized structure. Fortunately, 3D-bioprinting opens up new possibilities for constructing adaptable skin models with vascularization and complex macrostructures [37]. Some researchers are susceptible to using in situ skin bioprinting against in vitro bioprinting due to several limitations during the handling and implantation procedure. An in vitro skin bioprinting study discovered that certain reconstructed 3D-skin models exhibited significant fragile micro and macro-structures. This may result in structural impairments such as swelling, contraction, or distortion upon transplantation. Furthermore, in vitro bioprinting is subject to a significant risk of contamination during transportation and manual implantation [38].

3.2. In Situ Skin 3D-Bioprinting

To date, significant progress in tissue engineering has been proved by introducing in situ bioprinting technique. The basic principle for in situ bioprinting is performing a bioprinting method of pre-cultured cells directly onto the skin injury site and allowing for skin maturation at the wound area [35]. The in situ bioprinting approach provides a novel delivery bioinks approach for cell deposition at the injury site. Figure 6 shows the deposition of bioinks in a mouse wound by using the inkjet-based bioprinting technique. In situ bioprinting of the skin construct directly on the wound site is dependent on the patient’s body acting as a “bioreactor” for the functional maturation of the bioprinted tissue [39]. However, the wounds were scanned first to get accurate information on the wound topography, which was then used to direct the printing head to deposit the bioinks onto the injury site [40].
Ijms 23 00476 g006 550
Figure 6. In situ bioprinting for the wound by using inkjet-based bioprinting technique.
Overall, the laser wound scanner aids in the creation of a precise shape/map of the lost skin, and the bioinks will be printed out to this region [35]. The major advantage of the in situ bioprinting technique is that it facilitates the removal of artificial microenvironment formation, which is essential in newly formed tissue. In situ bioprinting approach provide rapid coverage towards the larger wound area [39].

4. Natural Biomaterials

A desirable property of bioinks should enhance the physicochemical properties, including the rheological, mechanical, chemical, and biological properties of the fabricated scaffold to mimic the native tissues. A hydrogel that resembles the composition of the ECM has received much attention. Natural-based bioinks have become the most favored bioinks for tissue engineering applications due to their non-immunogenic, biocompatibility, biodegradability, and hydrophilicity properties [41]. Table 1 summarizes the comparison of the biomaterial properties. On the other hand, synthetic-based bioinks provide better opportunities for tissue/organs construction [42]. The optimization of the bioinks should lead to an acceptable level of cellular activities, including cell migration, cell proliferation, cell viability, protein/gene expression, as in Figure 7.
Ijms 23 00476 g007 550
Figure 7. Cellular activities that the bioinks can influence.
Table 1. Properties of natural-based bioinks.

Type of Bioinks

Sources

Properties

References

DECM

Majority composed of ECM

dECM-based bioinks have viscoelastic behavior and rheological properties of dECMs, including shear viscosity and shear modulus that can preserve cells during printing. Besides, it is a biodegradable and low cytotoxicity biomaterials.

[43][44]

Collagen

Bovine, porcine, murine, and marine

Low viscosity, high shear stress, low viscosity, and weak mechanical strength.

[45][46][47]

Gelatin

Bovine, porcine

Has controllable mechanical properties depending on the concentrations, temperature-dependent, reversible state from solid to gel, and its challenging to optimize the temperature and its viscosity

[48][49]

Alginate

Algae

has high shear-thinning properties and a faster polymerization time after printing. However, alginate do not have cell adhesion sites

[50][51][52]

Cellulose

Plant or bacterial ECM

Naturally occurring, biocompatible, biodegradable, and abundant biopolymer, high solubility in water and numerous carboxyl groups

[53][54]

Silk

Silkworms and spiders

low concentration and viscosity, slow biodegradation rate

[52][55][56]

Fibrinogen

Plasma protein

Biocompatibility, biodegradability, adjustable mechanical properties, nanofibrous structural characteristics, and low viscosity properties

[57][58]

Chitosan

Chitin

Biocompatibility, antibacterial properties, thermosensitive, and low mechanical strength

[59][60][61]

This entry is adapted from the peer-reviewed paper 10.3390/ijms23010476

References

  1. He, P.; Zhao, J.; Zhang, J.; Li, B.; Gou, Z.; Gou, M.; Li, X. Bioprinting of skin constructs for wound healing. Burns Trauma 2018, 6, 1–10.
  2. Gao, Y.; Li, Z.; Huang, J.; Zhao, M.; Wu, J. In situformation of injectable hydrogels for chronic wound healing. J. Mater. Chem. B 2020, 8, 8768–8780.
  3. Maniţă, P.G.; García-Orue, I.; Santos-Vizcaíno, E.; Hernández, R.M.; Igartua, M. 3D Bioprinting of Functional Skin Substitutes for Chronic Wound Treatment: From Current Achievements to Future Goals. SSRN Electron. J. 2020, 14, 25.
  4. Patel, M.; Lantis, J.C., II. Fish skin acellular dermal matrix: Potential in the treatment of chronic wounds. Chronic Wound Care Manag. Res. 2019, 6, 59–70.
  5. Tort, S.; Demiröz, F.T.; Coşkun Cevher, Ş.; Sarıbaş, S.; Özoğul, C.; Acartürk, F. The effect of a new wound dressing on wound healing: Biochemical and histopathological evaluation. Burns 2020, 46, 143–155.
  6. Catanzano, O.; Quaglia, F.; Boateng, J.S. Wound dressings as growth factor delivery platforms for chronic wound healing. Expert Opin. Drug Deliv. 2021, 18, 737–759.
  7. Han, G.; Ceilley, R. Chronic Wound Healing: A Review of Current Management and Treatments. Adv. Ther. 2017, 34, 599–610.
  8. Smith, P.C.; Martínez, C.; Martínez, J.; McCulloch, C.A. Role of Fibroblast Populations in Periodontal Wound Healing and Tissue Remodeling. Front. Physiol. 2019, 10, 270.
  9. Ellis, S.; Lin, E.J.; Tartar, D. Immunology of Wound Healing. Curr. Dermatol. Rep. 2018, 7, 350–358.
  10. Jara, C.P.; Wang, O.; Paulino do Prado, T.; Ismail, A.; Fabian, F.M.; Li, H.; Velloso, L.A.; Carlson, M.A.; Burgess, W.; Lei, Y.; et al. Novel fibrin-fibronectin matrix accelerates mice skin wound healing. Bioact. Mater. 2020, 5, 949–962.
  11. Tottoli, E.M.; Dorati, R.; Genta, I.; Chiesa, E.; Pisani, S.; Conti, B. Skin wound healing process and new emerging technologies for skin wound care and regeneration. Pharmaceutics 2020, 12, 735.
  12. Przekora, A. A Concise Review on Tissue Engineered Artificial Skin Grafts for Chronic Wound Treatment: Can We Reconstruct Functional Skin Tissue In Vitro? Cells 2020, 9, 1622.
  13. Xu, Z.; Han, S.; Gu, Z.; Wu, J. Advances and Impact of Antioxidant Hydrogel in Chronic Wound Healing. Adv. Healthc. Mater. 2020, 9, 1901502.
  14. Sallehuddin, N.; Nordin, A.; Idrus, R.B.H.; Fauzi, M.B. Nigella sativa and its active compound, thymoquinone, accelerate wound healing in an in vivo animal model: A comprehensive review. Int. J. Environ. Res. Public Health 2020, 17, 4160.
  15. Avishai, E.; Yeghiazaryan, K.; Golubnitschaja, O. Impaired wound healing: Facts and hypotheses for multi-professional considerations in predictive, preventive and personalised medicine. EPMA J. 2017, 8, 23–33.
  16. Ezhilarasu, H.; Vishalli, D.; Dheen, S.T.; Bay, B.H.; Kumar Srinivasan, D. Nanoparticle-based therapeutic approach for diabetic wound healing. Nanomaterials 2020, 10, 1234.
  17. Singh, S.; Young, A.; McNaught, C.E. The physiology of wound healing. Surgery 2017, 35, 473–477.
  18. Kim, M.H.; Liu, W.; Borjesson, D.L.; Curry, F.R.E.; Miller, L.S.; Cheung, A.L.; Liu, F.T.; Isseroff, R.R.; Simon, S.I. Dynamics of neutrophil infiltration during cutaneous wound healing and infection using fluorescence imaging. J. Investig. Dermatol. 2008, 128, 1812–1820.
  19. Zulkiflee, I.; Fauzi, M.B. Gelatin-polyvinyl alcohol film for tissue engineering: A concise review. Biomedicines 2021, 9, 979.
  20. Javaid, M.; Haleem, A. 3D bioprinting applications for the printing of skin: A brief study. Sens. Int. 2021, 2, 100123.
  21. Correia Carreira, S.; Begum, R.; Perriman, A.W. 3D Bioprinting: The Emergence of Programmable Biodesign. Adv. Healthc. Mater. 2020, 9, 1900554.
  22. Masri, S.; Fauzi, M. Current Insight of Printability Quality Improvement Strategies in Natural-Based Bioinks for Skin Regeneration and wound healing. Polymers 2021, 13, 1011.
  23. Salleh, A.; Fauzi, M.B. The in vivo, in vitro and in ovo evaluation of quantum dots in wound healing: A review. Polymers 2021, 13, 191.
  24. Zhong, S.P.; Zhang, Y.Z.; Lim, C.T. Tissue scaffolds for skin wound healing and dermal reconstruction. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 2010, 2, 510–525.
  25. Stunova, A.; Vistejnova, L. Dermal fibroblasts—A heterogeneous population with regulatory function in wound healing. Cytokine Growth Factor Rev. 2018, 39, 137–150.
  26. Salimian Rizi, V. Ce Pte Us Pt. Mater. Res. Express 2019, 1–12.
  27. Chowdhury, S.R.; Jing, L.S.; Zolkafli, M.N.H.B.; Zarin, N.A.B.M.A.; Abdullah, W.A.B.W.; Md Mothar, N.A.B.; Maarof, M.; Abdullah, N.A.H. Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing. Sains Malays. 2019, 48, 637–644.
  28. Bader, D.L.; Worsley, P.R. Technologies to monitor the health of loaded skin tissues. Biomed. Eng. Online 2018, 17, 40.
  29. Woo, W.M. Skin structure and biology. Augment. Cust. Strateg. CRM Digit. Age. 2019, pp. 1–14. Available online: https://onlinelibrary.wiley.com/doi/abs/10.1002/9783527814633.ch1 (accessed on 13 November 2021).
  30. Lee, H.R.; Park, J.A.; Kim, S.; Jo, Y.; Kang, D.; Jung, S. 3D microextrusion-inkjet hybrid printing of structured human skin equivalents. Bioprinting 2021, 22, e00143.
  31. Hu, H.; Xu, F.-J. Rational design and latest advances of polysaccharide-based hydrogels for wound healing. Biomater. Sci. 2020, 8, 2084–2101.
  32. Hospodiuk, M.; Dey, M.; Sosnoski, D.; Ozbolat, I.T. The bioink: A comprehensive review on bioprintable materials. Biotechnol. Adv. 2017, 35, 217–239.
  33. Desanlis, A.; Albouy, M.; Rousselle, P.; Thepot, A.; Desanlis, A.; Albouy, M.; Rousselle, P.; Thepot, A.; Santos, M. Dos Validation of an implantable bioink using mechanical extraction of human skin cells: First steps to a 3D bioprinting treatment of deep second degree burn. J. Tissue Eng. Regen. Med. 2021, 15, 37–48.
  34. Seet, W.T.; Maarof, M.; Anuar, K.K.; Chua, K.; Wahab, A.; Irfan, A.; Ng, M.H.; Aminuddin, B.S.; Hj, B.; Ruszymah, I. Shelf-Life Evaluation of Bilayered Human Skin Equivalent, MyDermTM. PLoS ONE 2012, 7, e40978.
  35. Varkey, M.; Visscher, D.O.; van Zuijlen, P.P.M.; Atala, A.; Yoo, J.J. Skin bioprinting: The future of burn wound reconstruction? Burn. Trauma 2019, 7, 1–12.
  36. Augustine, R. Skin bioprinting: A novel approach for creating artificial skin from synthetic and natural building blocks. Prog. Biomater. 2018, 7, 77–92.
  37. Kim, B.S.; Kwon, Y.W.; Kong, J.S.; Park, G.T.; Gao, G.; Han, W.; Kim, M.B.; Lee, H.; Kim, J.H.; Cho, D.W. 3D cell printing of in vitro stabilized skin model and in vivo pre-vascularized skin patch using tissue-specific extracellular matrix bioink: A step towards advanced skin tissue engineering. Biomaterials 2018, 168, 38–53.
  38. Singh, S.; Choudhury, D.; Yu, F.; Mironov, V.; Naing, M.W. In situ bioprinting—Bioprinting from benchside to bedside? Acta Biomater. 2020, 101, 14–25.
  39. Murphy, S.V.; De Coppi, P.; Atala, A. Opportunities and challenges of translational 3D bioprinting. Nat. Biomed. Eng. 2020, 4, 370–380.
  40. Askari, M.; Naniz, M.A.; Kouhi, M.; Saberi, A.; Zolfagharian, A.; Bodaghi, M. Biomaterials Science. Biomater. Sci. 2021, 9, 535–573.
  41. Xu, J.; Zheng, S.; Hu, X.; Li, L.; Li, W.; Parungao, R.; Wang, Y.; Nie, Y.; Liu, T.; Song, K. Advances in the Research of Bioinks Based on Natural Collagen, Polysaccharide and Their Derivatives for Skin 3D Bioprinting. Polymers 2020, 12, 1237.
  42. Ahadian, S.; Khademhosseini, A. Handheld Skin Printer: In-Situ Formation of Planar Biomaterials and Tissues. Physiol. Behav. 2019, 176, 139–148.
  43. Khoshnood, N.; Zamanian, A. Decellularized extracellular matrix bioinks and their application in skin tissue engineering. Bioprinting 2020, 20, e00095.
  44. Lee, B.H.; Lum, N.; Seow, L.Y.; Lim, P.Q.; Tan, L.P. Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications. Materials 2016, 9, 797.
  45. Sheehy, E.J.; Cunniffe, G.M.; Brien, F.J.O. Collagen-Based Biomaterials for Tissue Regeneration and Repair 5. In Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair; Elsevier: Amsterdam, The Netherlands, 2018.
  46. Mariod, A.A.; Adam, H.F. Review: Gelatin, source, extraction and industrial applications. Acta Sci. Pol. Technol. Aliment. 2013, 12, 135–147.
  47. Dalby, M.J. Materials Today Bio A tough act to follow: Collagen hydrogel modi fi cations to improve mechanical and growth factor loading capabilities. Mater. Today Bio 2021, 10, 100098.
  48. Jang, K.S.; Park, S.J.; Choi, J.J.; Kim, H.N.; Shim, K.M.; Kim, M.J.; Jang, I.H.; Jin, S.W.; Kang, S.S.; Kim, S.E.; et al. Therapeutic efficacy of artificial skin produced by 3d bioprinting. Materials 2021, 14, 5177.
  49. Wang, Y.; Rudym, D.D.; Walsh, A.; Abrahamsen, L.; Kim, H.J.; Kim, H.S.; Kirker-Head, C.; Kaplan, D.L. In vivo degradation of three-dimensional silk fibroin scaffolds. Biomaterials 2008, 29, 3415–3428.
  50. Gopinathan, J.; Noh, I. Recent trends in bioinks for 3D. Biomater. Res. 2018, 22, 11.
  51. Sarker, B.; Rompf, J.; Silva, R.; Lang, N.; Detsch, R.; Kaschta, J.; Fabry, B.; Boccaccini, A.R. Alginate-based hydrogels with improved adhesive properties for cell encapsulation. Int. J. Biol. Macromol. 2015, 78, 72–78.
  52. Mohamed, A.L.; Soliman, A.A.F.; Abobakr, E.; Abou-zeid, N.Y.; Nada, A.A. International Journal of Biological Macromolecules Hydrogel bioink based on clickable cellulose derivatives: Synthesis, characterization and in vitro assessment. Int. J. Biol. Macromol. 2020, 163, 888–897.
  53. Chouhan, D.; Mandal, B.B. Silk biomaterials in wound healing and skin regeneration therapeutics: From bench to bedside. Acta Biomater. 2020, 103, 24–51.
  54. Pérez-Rigueiro, J.; Elices, M.; Plaza, G.R.; Guinea, G.V. Similarities and differences in the supramolecular organization of silkworm and spider silk. Macromolecules 2007, 40, 5360–5365.
  55. Mabrouk, M.; El-bassyouni, G.T.; Beherei, H.H. Inorganic additives to augment the mechanical properties of 3D-printed systems. In Advanced 3D-Printed Systems and Nanosystems for Drug Delivery and Tissue Engineering; Elsevier: Amsterdam, The Netherlands, 2020.
  56. Zarrintaj, P.; Manouchehri, S.; Ahmadi, Z.; Saeb, M.R.; Urbanska, A.M.; Kaplan, D.L.; Mozafari, M. Agarose-based biomaterials for tissue engineering. Carbohydr. Polym. 2018, 187, 66–84.
  57. Daikuara, L.Y.; Chen, X.; Yue, Z.; Skropeta, D.; Wood, F.M.; Fear, M.W.; Wallace, G.G. 3D Bioprinting Constructs to Facilitate Skin Regeneration. Adv. Funct. Mater. 2021, 2105080.
  58. Veiga, A.; Silva, I.V.; Duarte, M.M.; Oliveira, A.L. Current trends on protein driven bioinks for 3d printing. Pharmaceutics 2021, 13, 1444.
  59. Xu, J.; Fang, H.; Zheng, S.; Li, L.; Jiao, Z.; Wang, H.; Nie, Y.; Liu, T.; Song, K. A biological functional hybrid scaffold based on decellularized extracellular matrix/gelatin/chitosan with high biocompatibility and antibacterial activity for skin tissue engineering. Int. J. Biol. Macromol. 2021, 187, 840–849.
  60. Suo, H.; Zhang, J.; Xu, M.; Wang, L. Low-temperature 3D printing of collagen and chitosan composite for tissue engineering. Mater. Sci. Eng. C 2021, 123, 111963.
  61. Ahmed, S.; Ikram, S. Chitosan Based Scaffolds and Their Applications in Wound Healing. Achiev. Life Sci. 2016, 10, 27–37.
More
© Text is available under the terms and conditions of the Creative Commons Attribution (CC BY) license; additional terms may apply. By using this site, you agree to the Terms and Conditions and Privacy Policy.
This entry is offline, you can click here to edit this entry!
Video Production Service
Feedback