Classical taxonomy underlies most taxonomic studies of species description in the subfamily Triatominae. The morphological and morphometric studies applied in the last described taxa are: morphological study of the head, thorax, abdomen, and male and female genitalia (with optical microscopy (OM) and/or scanning electronic microscopy (SEM)), and morphometric study of the head, thorax, abdomen and appendices (using OM) [5,6,7,15,16,17,132].

Although the use of morphological and morphometric characters is essential to describe a new taxon (since the diagnosis of the species needs to be made based on specimens that will be deposited, such as vouchers, in entomological collections), evolutionary events of cryptic speciation [14] and phenotypic plasticity [14] present in the subfamily Triatominae can make it difficult to diagnose a taxon only by morphological studies. Classic examples of this can be seen in the genus Rhodnius Stål, 1859: R. montenegrensis Rosa et al., 2012 [13] and R. marabaensis Souza et al., 2017 [15] represent two of the four paraphyletic strains of R. robustus Larrousse, 1927 [134,135] (the application of integrative taxonomy allowed description of the species from specimens initially characterized as R. robustus [136]). On the other hand, was demonstrated that R. taquarussiensis Rosa et al., 2017 (species described by integrative taxonomy [20]) represented only an intraspecific polymorphism of R. neglectus Lent, 1954 [19] (from studies of molecular taxonomy combined with experimental crosses it was possible to synonymize the species [19]).

Morphological convergence events can also hinder the classic taxonomy of these vectors [129]. The paraphyletic genus Triatoma Laporte, 1832 needs several studies from a taxonomic and systematic point of view [137]. Triatoma tibiamaculata (Pinto, 1926), for example, is a species that has morphological characteristics that bring it together and groups it (until now) as a Triatoma [138]. However, the generic status of this vector has been questioned several times [134,137,138]—since it presents cytogenetic [139], structural [140] and phylogenetic [137,138] characteristics that bring it closer to Panstrongylus (which highlights the importance of studies with integrative taxonomy).

The first phylogenetic trees with molecular markers were published only in 1998 [141], giving rise to the phylogenetic systematics and molecular taxonomy of these vectors. Although no species of triatomine has been described by molecular taxonomy, the combination of phylogenetic analyses with morphological and morphometric studies in species description studies (integrative taxonomy) has been a trend in the last decade [5,13,14,15,16,17], since it provides greater reliability of the specific status of the taxa and allows, above all, to understand the evolutionary history of the species.

In addition to the contributions mentioned above, molecular taxonomy and phylogenetic systematics allowed the evaluation and re-validation of the taxonomic status of some species: reinclusion of Linshcosteus Distant, 1904 genus in Triatomini tribe (extinguishing the Linshcosteini tribe) [30]; inclusion of Psammolestes Bergroth, 1911 species in the genus Rhodnius [30] (proposal not accepted by the scientific community due to the differences that support the generic status of Psammolestes [17]); inclusion of the species T. flavida Neiva, 1911, and N. obscura Maldonado & Farr, 1962 in the genus Nesotriatoma Usinger, 1944 [142]; confirmation of the generic status of Nesotriatoma [132]; inclusion of species T. spinolai Porter, 1934, M. gajardoi Frias, Henry & Gonzalez, 1998, T. eratyrusiformis Del Ponte, 1929, and T. breyeri Del Ponte, 1929 in the genus Mepraia Mazza, Gajardo & Jörg, 1940 [142] (partially accepted suggestion, being the Mepraia genus currently composed of M. spinolai, M. gajardoi, and M. parapatrica Frías-Lasserre, 2010 [4,143]); confirmation of the generic status of Mepraia [137]; and inclusion of T. dimidiata (Latreille, 1811) in the Meccus Stål, 1859 genus (genus that later was considered invalid and the Meccus species started to be considered as Triatoma [137,144,145]).

Although the International Code of Zoological Nomenclature does not consider groupings of triatomines to be complexes or subcomplexes [146], Justi et al. [137] suggests that these groupings should represent monophyletic groups. In the genus Triatoma, for example, studies based on phylogenetic systematics evaluated the position of several species that had been grouped mainly by geographic distribution and morphological similarities and proposed regrouping and/or the creation of new monophyletic groups [137,147,148]. Species well defined as natural groups (monophyletic) are currently the T. brasiliensis [149,150], T. sordida [151], T. rubrovaria [151], T. infestans [137], and T. vitticeps [148] subcomplexes.

The data integration in the integrative taxonomy can be done by cumulation or congruence [152]. The use of combined tools to delimit a species of triatomine occurred for the first time in 1998 by Frias et al. [111] who combined morphological, morphometric, cytogenetic, and reproductive barriers data to describe M. gajardoi . However, only in the last decade has the integrative taxonomy has been more applied in the study of these vectors.

This tendency to integrate different analyses to characterize a taxon, made it possible to resolve ancient taxonomic issues, such as the description by T. mopan Dorn et al. (2018) and T. huehuetenanguensis Lima-Cordón et al. (2019) from specimens initially characterized as T. dimidiata [16,17,153,154] and the recent description of T. rosai Alevi et al., 2020 from the allopatric population of T. sordida (Stål, 1859) from Argentina [5,155,156]. In addition, the specific status of T. bahiensis Sherlock & Serafim, 1967 (a species that for more than three decades has been synonymous with T. lenti Sherlock & Serafim, 1967 [101]) has been revalidated based on integrative taxonomy [149].

On the other hand, even if the integrative taxonomy provides more robustness in the characterization of the new taxa (decreasing the chance of synonymization), does not prevent this event can occur (as mentioned above for R. taquarussuensis which has been synonymous with R. neglectus Lent, 1954 [19]). Although morphological, morphometric, and cytogenetic intraspecific variation had been described in the genus Rhodnius [157,158], the description of R. taquarussuensis was based on these factors [20]. Thus, synonymization event occurred through phylogenetic analyses and experimental crosses [19]. We suggest that integrative taxonomy work should include molecular studies and, whenever possible, reproductive barriers to confirm the taxon specific status following the biological concept of species [159,160,161].

In general, most articles of description based on integrative taxonomy combine only morphological and morphometric data with molecular analyses. However, it is worth mentioning that in 2020 the description of T. rosai was published based on morphometric, morphological, molecular data, and experimental crosses that have been combined with information from the literature about the species (cytogenetic data [155,156], electrophoresis pattern [155], cuticular hydrocarbons pattern [162], geometric morphometry [163], cycle, and average time of life [164,165,166] as well as geographic distribution [18,42,43,44,50,51]), becoming the most complete article of species description of the subfamily Triatominae [5].