Transposon-based mutagenesis is the generation of heritable genetic changes through the insertion or movement of transposable elements. In plants, insertion into a gene can disrupt its activity, and the inserted sequence provides a molecular tag for locating the affected genomic region [1]. Introduced transposition systems can mobilize a nonautonomous element when the necessary transposition functions are supplied separately [2]. Collections of plants carrying different insertions contain mutations distributed across multiple genes; the insertion sites and associated phenotypes characterize individual lines [3]. Transposable elements can modify coding sequences, alter nearby regulatory sequences, or promote genome rearrangements. Their effects include changes in the amount, location, or timing of gene expression, as well as changes in protein sequence and gene function [4].
- transposable elements
- insertional mutagenesis
- plant genetics
- transposition
References
- John Doebley; Adrian Stec; Lauren Hubbard; The Evolution of Apical Dominance in Maize. Nat. 1997, 386, 485-488, 10.1038/386485a0.
- David A. Jones; Colwyn M. Thomas; Kim E. Hammond-Kosack; Peter J. Balint-Kurti; Jonathan D. G. Jones; Isolation of the Tomato Cf-9 Gene for Resistance to Cladosporium fulvum by Transposon Tagging. Sci. 1994, 266, 789-793, 10.1126/science.7973631.
- Yunhe Jiang; Zhaoxia Cai; Weibo Xie; Tuan Long; Huihui Yu; Qifa Zhang; Rice Functional Genomics Research: Progress and Implications for Crop Genetic Improvement. Biotechnol. Adv. 2012, 30, 1059-1070, 10.1016/j.biotechadv.2011.08.013.
- Damon Lisch; How Important are Transposons for Plant Evolution?. Nat. Rev. Genet. 2012, 14, 49-61, 10.1038/nrg3374.