Enzyme adsorption is the accumulation of enzyme molecules at a material surface through noncovalent interactions between the protein and the interface. Adsorbed enzymes form a carrier-associated catalytic preparation in which the support retains protein during reaction and separation [1][2]. Surface area, pore architecture, and protein–surface contacts determine how enzyme molecules occupy the material and which portions remain exposed to solution [3]. Hydrophobic supports can bind lipases through interfacial activation, stabilizing a surface-associated conformation with an accessible catalytic region. Changes in solvent composition or the presence of detergents can release the adsorbed enzyme [4]. Adsorption therefore produces a reversible association. Enzyme loading, the proportion of activity retained, and accessibility of substrates describe distinct properties of the resulting immobilized preparation [5].
- enzyme immobilization
- protein–surface interactions
- noncovalent binding
- desorption
References
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- Rafael C. Rodrigues; Jose J. Virgen-Ortíz; José C.S. dos Santos; Ángel Berenguer-Murcia; Andres R. Alcantara; Oveimar Barbosa; Claudia Ortiz; Roberto Fernandez-Lafuente; Immobilization of Lipases on Hydrophobic Supports: Immobilization Mechanism, Advantages, Problems, and Solutions. Biotechnol. Adv. 2019, 37, 746-770, 10.1016/j.biotechadv.2019.04.003.
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