A multienzyme complex is a physical assembly of multiple enzyme components that perform coordinated catalytic reactions. Covalently attached carrier groups or mobile protein domains can transfer intermediates between active sites within the assembly [1]. The organization may include a central oligomeric core bearing repeated catalytic components, with additional enzymes attached around it [2]. Some complexes channel an intermediate directly from one reaction site to the next without complete equilibration with the surrounding solution. Complexes can be stable assemblies or form through transient associations among their components [3]. Internal tunnels provide another route for intermediate transfer, as in an assembly that connects two catalytic sites while coordinating their activities through conformational changes [4]. Protein interfaces and flexible linkers organize these spatial and kinetic relationships [5].
- enzyme assembly
- catalytic subunits
- protein scaffolds
- coordinated catalysis
References
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- Andrea Mattevi; Galya Obmolova; Egbert Schulze; Kor H. Kalk; Adrie H. Westphal; Arie de Kok; Wim G. J. Hol; Atomic Structure of the Cubic Core of the Pyruvate Dehydrogenase Multienzyme Eomplex. Science 1992, 255, 1544-1550, 10.1126/science.1549782.
- Y.-H. Percival Zhang; Substrate Channeling and Enzyme Complexes for Biotechnological Applications. Biotechnol. Adv. 2011, 29, 715-725, 10.1016/j.biotechadv.2011.05.020.
- Michael F. Dunn; Dimitri Niks; Huu Ngo; Thomas R.M. Barends; Ilme Schlichting; Tryptophan Synthase: the Workings of a Channeling Nanomachine. Trends Biochem. Sci. 2008, 33, 254-264, 10.1016/j.tibs.2008.04.008.
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