Enzyme adsorption is the accumulation of enzyme molecules at a material surface through noncovalent interactions between the protein and the interface. Adsorbed enzymes form a carrier-associated catalytic preparation in which the support retains protein during reaction and separation [1][2]. Surface area, pore architecture, and protein–surface contacts determine how enzyme molecules occupy the material and which portions remain exposed to solution [3]. Hydrophobic supports can bind lipases through interfacial activation, stabilizing a surface-associated conformation with an accessible catalytic region. Changes in solvent composition or the presence of detergents can release the adsorbed enzyme [4]. Adsorption therefore produces a reversible association. Enzyme loading, the proportion of activity retained, and accessibility of substrates describe distinct properties of the resulting immobilized preparation [5].
- enzyme immobilization
- protein–surface interactions
- noncovalent binding
- desorption
Enzyme Immobilization Techniques·Molecular Biology·Biochemistry, Genetics and Molecular Biology·Life Sciences
References
- Robert DiCosimo; Joseph McAuliffe; Ayrookaran J. Poulose; Gregory Bohlmann; Industrial Use of Immobilized Enzymes. Chem. Soc. Rev. 2013, 42, 6437-6474, 10.1039/c3cs35506c.
- Ulf Hanefeld; Lucia Gardossi; Edmond Magner; Understanding Enzyme Immobilisation. Chem. Soc. Rev. 2008, 38, 453-468, 10.1039/b711564b.
- Shakeel Ahmed Ansari; Qayyum Husain; Potential Applications of Enzymes Immobilized on/in Nano Materials: A Review. Biotechnol. Adv. 2012, 30, 512-523, 10.1016/j.biotechadv.2011.09.005.
- Rafael C. Rodrigues; Jose J. Virgen-Ortíz; José C.S. dos Santos; Ángel Berenguer-Murcia; Andres R. Alcantara; Oveimar Barbosa; Claudia Ortiz; Roberto Fernandez-Lafuente; Immobilization of Lipases on Hydrophobic Supports: Immobilization Mechanism, Advantages, Problems, and Solutions. Biotechnol. Adv. 2019, 37, 746-770, 10.1016/j.biotechadv.2019.04.003.
- Roger A. Sheldon; Sander van Pelt; Enzyme Immobilisation in Biocatalysis: Why, What and How. Chem. Soc. Rev. 2013, 42, 6223-6235, 10.1039/c3cs60075k.