Liquid chromatography operated at intermediate pressures—higher than gravity columns and lower than analytical HPLC—constitutes medium-pressure liquid chromatography (MPLC). Glass or plastic columns packed with relatively large-particle silica or bonded phases are eluted by a pump at typical pressures of a few to tens of bar; the purpose is preparative isolation of milligram-to-gram quantities rather than high-efficiency analytical quantification [1]. The retention mechanisms are the same as in other liquid chromatographies (normal phase, reversed phase, silver-ion, or ion exchange); the defining attributes are the pressure range, particle size, and preparative scale [2]. Conceptual boundaries exclude flash chromatography on disposable cartridges when that technique is distinguished by still lower pressure and shorter beds, analytical HPLC, and gravity column chromatography. Essential features are a pumped liquid mobile phase, a packed bed designed for load rather than maximum plate number, and a fraction-collection scheme [3]. Combinations with macroporous-resin pre-fractionation remain MPLC at the resolving step [4].
- medium-pressure liquid chromatography
- MPLC
- preparative chromatography
- packed column
Analytical Chemistry and Chromatography • Spectroscopy • Chemistry • Physical Sciences
References
- Rong Wang; Xiaoguo Peng; Liming Wang; Beibei Tan; Jiangyun Liu; Yulin Feng; Shilin Yang; Preparative purification of peoniflorin and albiflorin from peony rhizome using macroporous resin and medium‐pressure liquid chromatography. J. Sep. Sci. 2012, 35, 1985-1992, 10.1002/jssc.201200120.
- Petra Weber; Matthias Hamburger; Nina Schafroth; Olivier Potterat; Flash chromatography on cartridges for the separation of plant extracts: Rules for the selection of chromatographic conditions and comparison with medium pressure liquid chromatography. Fitoter. 2011, 82, 155-161, 10.1016/j.fitote.2010.08.013.
- Pankaj P. Daramwar; Prabhakar Lal Srivastava; Balaraman Priyadarshini; Hirekodathakallu V. Thulasiram; Preparative separation of α- and β-santalenes and (Z)-α- and (Z)-β-santalols using silver nitrate-impregnated silica gel medium pressure liquid chromatography and analysis of sandalwood oil. Anal. 2012, 137, 4564-4570, 10.1039/c2an35575b.
- Yun Wang; Hui Liu; Lifeng Shen; Lan Yao; Yinlian Ma; Dingrong Yu; Jianhong Chen; Puling Li; Ying Chen; Cun Zhang; et al. Isolation and purification of six iridoid glycosides from gardenia jasminoides fruit by medium‐pressure liquid chromatography combined with macroporous resin chromatography. J. Sep. Sci. 2015, 38, 4119-4126, 10.1002/jssc.201500705.