Application of Citric Acid in the Food Industry: History
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Ewelina Książek

Citric acid finds broad applications in various industrial sectors, such as the pharmaceutical, food, chemical, and cosmetic industries. The bioproduction of citric acid uses various microorganisms, but the most commonly employed ones are filamentous fungi such as Aspergillus niger and yeast Yarrowia lipolytica.

  • citric acid
  • food additives
  • acidity regulator

1. Introduction

Citric acid (CA), also known as 2-hydroxypropane-1,2,3-tricarboxylic acid, is found in plant and animal tissues such as blood, bone, and muscle. For living organisms, citric acid is one of the essential carboxylic acids in the Krebs cycle, a series of reactions that oxidize glucose into carbon dioxide and water, releasing energy. Due to its harmless nature and chelating and sequestering properties for metal ions, citric acid has applications in the food, pharmaceutical, chemical, and even metallurgical industries [1][2]. The annual global production of citric acid currently reaches approximately 2.8 million tons, and the citric acid market is one of the fastest-growing segments in the food additive industry [3]. The continuous growth in citric acid production is attributed to its wide-ranging applications, not only in the food and pharmaceutical industries but also in biopolymer production, environmental protection, and biomedicine [4][5].
In industrial citric acid production, the dominant method is submerged fermentation involving strains of Aspergillus niger, yeast Yarrowia lipolytica, and some bacterial strains [6]. Aspergillus niger is considered the best among microorganisms in the commercial synthesis of citric acid due to its high production efficiency [7][8]. The development of citric acid production has significantly increased since the last century, thanks to biotechnology, which provides knowledge about fermentation techniques and product recovery; biochemistry, which provides insights into various factors influencing citric acid synthesis and inhibition; and molecular regulatory mechanisms and strategies to enhance citric acid production efficiency [1][9].

2. Physical and Chemical Properties

Citric acid [77–92–2], according to IUPAC nomenclature (International Union of Pure and Applied Chemistry), is also known as 2-hydroxypropane-1,2,3-tricarboxylic acid. Citric acid is a polyprotic α-hydroxy acid but can also be classified as a β-hydroxy acid (Figure 1) [8][10]. It is present in plants, animal cells, and physiological fluids. In small quantities, citric acid is found in citrus fruits, especially lemons and limes. In amounts exceeding 1% of the dry weight of the product, it is present in lemons (4–8%), blackberries (1.5–3.0%), grapefruits (1.2–2.1%), as well as oranges, raspberries, and strawberries in the range of 0.6–1.3% [11][12][13].
Figure 1. Chemical structure of citric acid.
Citric acid is an organic compound, a tricarboxylic hydroxy acid, with three carboxylic functional groups. It is a triprotic compound that undergoes three constant dissociations, which allows it to form three types of salts and exhibit buffering properties. The chemical and physical properties of citric acid are presented in Table 1 [14][15]. Citric acid forms crystalline mono-, di-, and tri-basic salts with various cations. From a technological perspective, the most important are calcium citrate, potassium citrate, and sodium citrate [16].
Citric acid is a weak acid in two crystalline forms: Anhydrous citric acid (C6H8O7) and monohydrated citric acid (C6H8O7·H2O). Anhydrous citric acid crystallizes from a hot concentrated solution above 36.6 °C, forming a white crystalline powder. On the other hand, monohydrated citric acid crystallizes from a cold solution at temperatures below 36.6 °C, forming colorless, transparent crystals [16][17]. Anhydrous citric acid absorbs a small amount of water at 25 °C and relative humidity in the 25 to 50% range. If the humidity is between 50% and 75%, it absorbs water significantly, while approaching 75% relative humidity takes the form of a monohydrate. The anhydrous form of citric acid is obtained when the relative humidity is less than 40%. Monohydrated citric acid slightly absorbs moisture at a relative humidity of 65–75% [17].
Citric acid is highly soluble in water and organic solvents such as ethanol, 2-propanol, ether, ethyl acetate, 1.4-dioxane, tetrahydrofuran, acetonitrile, and ethanol-water mixtures [18]. It has a higher solubility in alcohol than in water. Adding alcohol to an aqueous solution significantly increases the solubility of citric acid [19][20]. The solubility of citric acid in different solvents can be ranked as follows: Tetrahydrofuran < 1.4-dioxane < water < 2-propanol < ethanol < acetonitrile [21]. Citric acid does not dissolve in chloroform, toluene, benzene, carbon disulfide, or tetrachloride [17]. Its solubility increases with an increasing temperature of 20.55–60.05 °C [19][20][21].
When heated to 150 °C, citric acid remains stable, losing only its crystalline water. Above 175 °C, it undergoes a melting and decomposition process. Dehydration of citric acid leads to the formation of trans-aconitic acid. It is assumed that further thermal transformations of trans-aconitic acid due to dehydration result in the production of aconitic anhydride or a mixture of both isomers [15][22].
Citric acid can chelate metal ions by forming bonds between the metal, carboxyl, and hydroxyl groups of the citric acid molecule. Citric acid and its salts form complexes with copper, nickel, iron, magnesium, zinc, and tin. This valuable property helps prevent changes in chemical potential, precipitation of solids, or changes in chemical properties [15][23].
Citric acid esterifies with alcohols under typical conditions in the presence of catalysts such as sulfuric acid, p-toluenesulfonic acid, or ion-exchange resin. The esterification reaction of citric acid with alcohols, occurring at temperatures above 150 °C, does not require the presence of a catalyst. Citric acid forms polyesters with polyalcohols such as sorbitol and mannitol. Interrupting the esterification reaction before completion results in the formation of free carboxylic groups, forming salts [15].
Table 1. Chemical and physical properties of citric acid.
Properties Characteristic References
Molar mass Anhydrous: 192.12 g∙mol−1
Monohydrate: 210.14 g∙mol−1		 [11]
Appearance and form powdery, colorless transparent crystals or white, granular, fine powder
Anhydrous: monoclinic holohedral crystals. Monohydrate: rhombic crystals		 [14]
Melting point Anhydrous: 153° C
Monohydrate: ≈100 °C		 [8]
Boiling point None, decomposition into water and CO2 > 175 °C [24]
Vapor pressure 1.7 × 10−8 mmHg at 25 °C [18]
Density Anhydrous: 1.665 g∙cm−3 at 20 °C
Monohydrate: 1.542 g∙cm−3 at 20 °C		 [14]
Octanol/water partition coefficient logPOW = −1.72 ± 0.4 at 20 °C [10]
Partition coefficient logP = −1.198 ± 0.4 w 25 °C [14]
dissociation constant pK1 = 3.128
pK2 = 4.761 at 25 °C
pK3 = 6.396		 [14]
Henry’s constant KH = 2.3 ×·10−7 Pam3∙mol−1 [25]
Solubility
  • In water, the solubility increases with temperature:
  • solubility is 54% at 10 °C.
  • solubility is 84% at 100 °C.
  • In alcohol, the solubility is 59.1 g per 100 milliliters (g/100 mL) at 25 °C
  • In a mixture of ether and alcohol, the solubility is 162 g per 100 milliliters (g/100 mL) at 25 °C
 [14]

2. Application of Citric Acid in the Food Industry

From a health quality perspective, citric acid, when used as a food additive, has been approved as generally recognized as safe (GRAS) by the FAO/WHO Expert Committee on Food Additives, and its Acceptable Daily Intake (ADI) does not require limitation [26]. Derivatives of citric acid, such as calcium citrate, iron citrate, manganese citrate, potassium citrate, sodium citrate, diammonium citrate, isopropyl citrate, and stearyl citrate, have also received GRAS status as food additives [13].
Citric acid is characterized by its low production cost, easy accessibility, non-toxicity, biocompatibility, universality, and the safety of its decomposition products. It finds wide applications in the food, pharmaceutical, biomedical, chemical, agricultural, and environmental protection industries [27]. In food products, citric acid serves various functions, including acidity regulation, preservation, antioxidant properties, emulsification, flavor and aroma enhancement, buffering, and antibacterial activity. Citric acid’s ability to chelate metal ions and its buffering properties, when combined with citrates, make it an ideal additive in food and nutraceutical production [28][29][30].
Citric acid plays a significant role as an antioxidant in oil production and in limiting the oxidation of lipids in meat processing. It inhibits lipid oxidation by forming bonds between pro-oxidative metal ions and the carboxyl or hydroxyl groups of the acid. The antioxidant activity of citric acid in food depends on the dose applied and increases with higher acid concentrations [31].
In meat processing, citric acid reduces the pink color and increases the brightness of heat-treated meat. In cooked meat, citric acid limits the endogenous pink color and the color induced by sodium nitrite and nicotinamide. Reduction of the pink color in meat may also result from the chelation of heme iron by citric acid, preventing heme from binding with ligands that cause a pink color [32][33].
Citric acid and its salts are widely used in the food industry to prevent enzymatic browning [34]. Enzymatic browning of fruits and vegetables is a phenomenon that reduces shelf life and influences consumer decisions [35][36].
Citric acid is used as an additive in rinse water before deep freezing and in fruit syrups [37]. Adding citric acid to stored fruits and vegetables positively affects color retention and organoleptic quality and extends their shelf life. It can also be combined with other anti-browning agents, such as ascorbic acid [38].
The inhibition of citric acid’s polyphenol oxidase (PPO) activity is due to its pH-lowering capacity. PPO activity gradually decreases with increasing citric acid concentrations. However, the citric acid concentration needed to inhibit PPO activity varies depending on the PPO activity and buffer solutions used [34]. Radish slices immersed in a 0.3% aqueous citric acid solution showed no browning during storage. Querioz et al. found that a citric acid concentration of 100 mM inhibited the PPO activity of cashew apples [35]. However, a 10 mM citric acid solution inhibited banana PPO [39].
Citric acid also contributes to a decrease in the thermodynamic parameters of polyphenol oxidase. This is believed to be due to a reduction in the stability of PPO and the number of non-covalent bonds in the enzyme’s structure, leading to changes in the protein’s secondary and tertiary structure [34].
Citric acid can be considered a substance capable of controlling cellular respiration and contributing to the better preservation of fruits and vegetables during storage [36][40].

2.1. Newly Emerging Applications of Citric Acid

Research into new applications of citric acid in various industries is currently the subject of many studies [41][42][43][44][45][46]. One of the new applications of citric acid is the production of household detergents. Citric acid chelates Mg2+ and Ca2+ ions responsible for water hardness and does not contribute to the eutrophication of aquatic systems, unlike phosphates used in detergents [47]. New and innovative applications of citric acid in the food industry and beyond are expected to lead to increased production.

2.1.1. Cross-linking Agent and Plasticizer

Citric acid can successfully be used in the process of cross-linking proteins [48], polysaccharides [49], and hydroxyapatite [50]. A breakthrough in using citric acid as a cross-linking agent came with the discovery by Rothenberg and Alberts from the University of Amsterdam. They demonstrated that glycerol and citric acid can polymerize, creating a water-soluble, biodegradable, and thermosetting resin. The combination of citric acid and glycerol at temperatures above 100 °C and below 130 °C under normal conditions leads to the formation of polyester resins through the Fischer esterification reaction [51].
The use of citric acid as a compatibilizer for various polysaccharides, including starch, thermoplastic starch, cotton, chitosan, and cellulose, is justified by its multi-carboxyl structure [52]. This allows it to be used as a cross-linking agent, plasticizer, and hydrolyzing agent [53].
The mechanism of the cross-linking reaction is based on the well-known Fischer esterification reaction between the carboxyl groups of citric acid and the hydroxyl groups of starch [54]. Citric acid can react with all three hydroxyl groups of starch. Esterification between starch and citric acid leads to mono-, di-, and tri-esters forming. Esterification primarily occurs within the branching points of amylopectin [55]. The formation of ester bonds can be catalyzed by reducing the pH or adding Lewis acids, which are chemical compounds capable of accepting an electron pair from a base [54]. In many previous studies on starch cross-linking, temperatures above 100 °C have initiated the cross-linking process [56]. Heating citric acid causes dehydration and the formation of an anhydride, which can react with starch to form starch citrate. With further heating, the citrate undergoes dehydration, and cross-linking can occur [57][58].
It is possible to conduct cross-linking reactions at lower temperatures (around 70 °C) using higher concentrations of citric acid. However, the efficiency of the reaction under these conditions is low because only a tiny amount of added citric acid participates in the cross-linking reaction. Furthermore, citric acid that has not reacted can act as a plasticizer [55]. As a plasticizer, citric acid increases the tensile strength of starch films. The improvement in tensile strength is more significant than when glycerol is used as a plasticizer [59]. Excessive citric acid concentration does not interact with starch molecules but can react with water, disrupting hydrogen bonds and reducing the matrix’s cohesion. This results in increased water solubility, susceptibility to deformation, and reduced thermal resistance [60].
FTIR spectroscopy and X-ray diffraction of starch films with citric acid have shown that citric acid can effectively inhibit starch recrystallization or retrogradation due to strong hydrogen bonding between starch and citric acid [54]. Additionally, citric acid-cross-linked starch films exhibit significantly higher tensile strength, up to 150% more than non-cross-linked films. However, achieving the appropriate increase in material strength requires an optimal amount of citric acid [61]. Citric acid concentrations below 5% act as a cross-linking agent and enhance the tensile strength of starch films. When the concentration increases from 5% to 30%, tensile strength decreases, but flexibility and material adhesiveness increase. This suggests that excess free citric acid is a plasticizer [62].
The main disadvantage of citric acid as a cross-linking and plasticizing agent in starch barrier films is starch degradation due to acid hydrolysis. Acid hydrolysis of starch glycosidic bonds involves the cleavage of these bonds, resulting in the protonation of oxygen and the addition of a water molecule, leading to the formation of a reducing sugar group. Effectively preventing starch hydrolysis during cross-linking in the presence of citric acid can be achieved by maintaining a pH of 4 or lower and a temperature below 105 °C [54].
As a cross-linking agent, citric acid strengthens bonds by incorporating covalent bonds that complement intermolecular hydrogen bonds, improving the resistance of starch films to moisture. Strong hydrogen bonds between the carboxyl groups of citric acid and the hydroxyl groups of starch result in improved interactions between molecules and reduced solubility of the films in water [60].
When citric acid is added in the range of 1% to 10% to thermoplastic starch, it significantly reduces water vapor permeability. This effect is due to replacing hydrophilic groups with hydrophobic ester groups that impede the diffusion of water vapor molecules through the matrix. However, water vapor permeability increases when the citric acid concentration exceeds 10%. This can be attributed to the plasticizing effect caused by an excess of citric acid. Increased citric acid concentration leads to enhanced chain mobility and increased interchain spaces, resulting from the attachment of free citric acid to the polymer chain. As a result, the water vapor diffusion coefficient increases, accelerating water vapor penetration through the starch films [63][64][65].
Starch films cross-linked with citric acid have higher thermal resistance [56]. The reason for this is that the cross-links are responsible for the resistance of cross-linked films. Cross-linked starch films exhibit significantly improved thermal resistance at temperatures above 320 °C [61].
The three carboxyl groups and one hydroxyl group in citric acid also allow it to cross-link glycerol, cellulose, and sebacic acid through condensation reactions, forming ester copolymers capable of drug delivery [8]. Gentamicin incorporated into the polymer effectively kills bacteria. Citric acid delivers ketoconazole as a cross-linking agent for beta-cyclodextrins on hydrogel hydroxypropylmethylcellulose (HPMC) membranes. The formation of drug-cyclodextrin complexes contributes to increased solubility and bioavailability of poorly soluble drugs [66].

2.1.2. Citric Acid in the Synthesis of Deep Eutectic Solvents

Deep eutectic solvents (DES) are homogeneous mixtures of two or more components capable of interacting with each other as a hydrogen bond acceptor (HBA) and a hydrogen bond donor (HBD). Deep eutectic solvent mixtures are formed by mixing two or more components in the appropriate molar ratio in the presence of heat. Additionally, this process does not require an additional purification step [67][68]. Deep eutectic solvents are one of the most promising discoveries in “green chemistry”. They can serve as an alternative to conventional organic solvents and have numerous advantages, such as renewability, reusability, biodegradability, non-toxicity, widespread availability, shallow vapor pressure, low flammability, and ease of preparation. Moreover, the components that produce deep eutectic solvents are inexpensive and safe [69][70].
Deep eutectic solvents have been divided into four types depending on their composition. Types I, II, and IV contain metal salts and are considered toxic and less sustainable than type III deep eutectic solvents. Type III deep eutectic solvents are synthesized from readily biodegradable and regenerable raw materials such as feed additive (choline chloride, ChCl), fertilizer (urea), antifreeze (ethylene glycol), sweetener (glycerol), and plant metabolites (sugars, sugar alcohols, and organic acids) [71]. The most frequently studied eutectics in the literature are type III, based on the combination of quaternary ammonium salts and a compound serving as a hydrogen bond donor. Type III is the most commonly used deep eutectic solvent due to the strong interaction of hydrogen bonds between the hydrogen bond acceptor (HBA) and the hydrogen bond donor (HBD). Many compounds have been successfully utilized to create deep eutectic solvents. HBAs are mainly quaternary ammonium or phosphonium salts, while HBDs are most commonly amides, alcohols, and carboxylic acids. Citric acid is one of the most commonly employed HBDs among carboxylic acids [68]. The most popular systems for producing deep eutectic solvents using choline chloride and citric acid are 1:1, 1:2, and 2:1 [72][73]. The presence of hydroxyl and carboxyl groups allows for the formation of sufficiently strong hydrogen bonds. Deep eutectic solvents based on choline chloride and carboxylic acids demonstrate greater extraction efficiency than traditional solvents such as water and ethanol [74].
Various molar ratios of choline chloride and citric acid monohydrate significantly influence the physicochemical properties of deep eutectic solvents. Adding citric acid monohydrate increases viscosity, surface tension, and density. Deep eutectic solvents with a higher molar ratio of choline chloride exhibit a higher melting point. Citric acid-based deep eutectic solvents can find broad industrial applications, particularly in extracting hydrophilic components from plant or animal materials [74].
In the studies conducted by Kurtulbaş et al., deep eutectic solvents were intentionally designed, incorporating a hydrogen bond donor (HBD) (glycerol and ethylene glycol) and a hydrogen bond acceptor (HBA) (citric acid) in a specified molar ratio (1:4) for the extraction of bioactive compounds (phenols and anthocyanins). In the current investigation, Hibiscus sabdariffa was extracted using microwave-assisted extraction (MAEX). The most effective extract from Hibiscus sabdariffa was obtained from a mixture of citric acid and ethylene glycol through microwave-assisted extraction [70].
Hu et al., investigated the molecular mechanisms of isoliquiritigenin extraction using deep eutectic solvents of choline chloride and citric acid. The results indicated that deep eutectic solvents exhibited higher efficiency in isoliquiritigenin extraction as an extraction solvent than ethanol with water. Additionally, the increased efficiency in isoliquiritigenin extraction was primarily attributed to the strong interaction between isoliquiritigenin and the extraction solvent and the rapid diffusion of isoliquiritigenin [68].

2.1.3. Antibacterial Agent

Using organic acids to control bacterial flora in food, extend shelf life, and improve the safety of plant- and animal-derived products has become a common practice in the food industry. In Europe, the legal basis for using organic acids as agents contributing to the safety of animal-derived products is Regulation 853/2004 of the European Parliament and the Council [75][76][77].
Citric acid effectively combats pathogenic microflora in fresh and processed pork, beef, poultry, and fresh vegetables and fruits [78][79][80][81][82]. Its antibacterial activity involves penetrating through the cell membranes, where the pH is higher than in the surrounding environment. The mechanism of citric acid’s antibacterial action is related to acidifying the cytoplasm, disrupting metabolic processes, or accumulating the dissociated acid anion to a toxic level [83][84]. Organic acids are weak acids, so they do not completely dissociate in an aqueous environment, and their microbiological activity depends on the degree of dissociation and the pH of the food product. Reducing the pH increases the concentration of the acid, reduces the polarity of the molecules, improves acid diffusion through microbial cell membranes into the cells, and consequently increases antibacterial activity [84][85]. The effectiveness of organic acids also depends on the acid concentration, acid properties, temperature, exposure time, and microbial susceptibility [82][86].
Citric acid in concentrations ranging from 0.1 to 3.0% restricts the growth of bacteria such as Listeria monocytogenes, Escherichia coli, Salmonella typhimurium, and Vibrio parahaemoliticus [75][78][80][82].
Citric acid exhibits synergistic effects when used in mixtures with other organic acids. A mixture of caprylic and citric acids significantly inhibits the growth of bacteria. The synergy between citric and caprylic acids is associated with the loss of cell membrane integrity and changes in its permeability. The mechanism of the synergistic action of both acids involves damaging or destabilizing the cell membrane, leading to increased permeability and, consequently, cell death. Damage to the bacterial membrane allows hydrogen ions to penetrate, resulting in a strong bactericidal effect [79][87][88].
Combining citric acid with other decontamination methods, such as ozonation, UV-C radiation, and ultrasonication, can significantly impact the inactivation of microorganisms in fresh food [89].
The effectiveness of citric acid’s antibacterial action varies and depends on many factors. Citric acid exhibits optimal antibacterial effects in a low pH environment, at low temperatures, and when used in high concentrations. Available literature sources report that using citric acid at concentrations above 2% may cause adverse sensory changes in food products [84][90]. The antibacterial effectiveness of citric acid also depends on the initial amount of microflora on the product’s surface. Depending on the initial bacterial count, citric acid reduces the number of microorganisms by 1 to 2 log cfu∙g−1 [75].

2.1.4. Deamidation of Gluten

Wheat gluten is widely used in the food industry, serving various purposes, such as emulsifiers and imparting cohesiveness and elasticity. However, its utility is limited due to its low solubility under neutral conditions. A practical method to enhance the properties of gluten is deamidation using carboxylic acids, including citric acid [91][92].
The deamidation reaction transforms amidic groups into carboxylic groups, mainly glutamine and asparagine residues. This transformation results in increased electrostatic repulsion, the disruption of hydrogen bonds, and the dissociation of polymers, ultimately improving the solubility of gluten. While treating gluten with citric acid, the availability of peptide bonds and hydrogen ions influences the competition between hydrolysis and deamidation. The degree of hydrolysis of deamidated gluten decreases with increased citric acid concentration and treatment time, contributing to an increase in soluble protein content after deamidation [45][93]. Deamidation by citric acid increases the solubility of gluten to around 70% at pH 7. The shift of the protein’s isoelectric point towards acidic pH confirms that deamidation increases the quantity of protein polyelectrolytes, resulting in improved solubility at neutral pH. Deamidation involves the cleavage of peptide bonds, indicating that it is primarily responsible for increasing protein solubility rather than hydrolysis [93][94].
Deamidation of gluten with citric acid significantly enhances its emulsifying, foaming, and elasticity properties. Emulsions stabilized with deamidated gluten feature smaller emulsion droplet sizes, indicating their ability to reduce surface tension. High emulsion stability results from the increased flexibility of the gliadin molecule or its molecular rearrangement [93]. The improvement in the foaming capacity of deamidated gluten is attributed to the increased molecule flexibility, leading to enhanced protein adsorption and anchoring at phase boundaries [95][96]. Deamidation also leads to changes in the secondary conformation of the protein, driven by increased electrostatic repulsion and a reduction in hydrogen bonds [94]. The secondary structure of gluten consists of 34.5% α-helices, 17.3% β-turns, and 44.8% β-sheets. Deamidation increases α-helices and β-turns while reducing β-sheets [92][95].
Citric acid exhibits a solid capability to break peptide bonds in gluten, which leads to a transformation in the tertiary structure of the protein. Protein fractions of gluten with a higher molecular weight are more susceptible to degradation than those with a lower molecular weight. After deamidation with citric acid, the presence of sulfhydryl groups in gluten has been confirmed, while the tertiary structure becomes less compact [95][96].
Deamidation with citric acid can contribute to the improvement of the nutritional properties of gluten. From a nutritional standpoint, gluten is not considered a good protein source due to its deficiency in lysine and threonine. Deamidation with citric acid increases the overall quantity of essential amino acids, including lysine [93].

2.1.5. Extractant

Citric acid can be an effective pectin extractor from fruit pomace instead of toxic mineral acids such as sulfuric or nitric acid. Pectin extraction using citric acid can reduce waste from fruit and vegetable processing and limit the harmful environmental impact of wastewater from conventional extraction methods [97][98].
The process of pectin extraction typically occurs at around 97 °C with a pH of 2.5 using water acidified with citric acid [30][97].
Extracting pectin from citrus fruit peels using citric acid allows for obtaining pectins with a high galacturonic acid content, essential for their application as a gelling agent. Pectins also have a high molecular weight, indicating a high content of neutral sugars. The viscosity of the extracted pectins increases with a higher concentration of citric acid. Although the extraction process may yield lower efficiency than traditional methods, it results in pectins of high purity [99].
The pectins extracted from cocoa husks were characterized by a high degree of acetylation, contained rhamnogalacturonan, and had side chains rich in galactose. Despite their high degree of acetylation, these pectins formed gels in a low pH environment and at a high glucose concentration, suggesting their potential use as an additive in acidic products [100].

2.1.6. Inhibition of Protein Adhesion

The issue of protein adhesion to steel surfaces poses numerous challenges in food production and processing. Proteins adhering to equipment surfaces can serve as a nutrient source for microorganisms, leading to product contamination. Removing allergens and preventing cross-contamination is a critical point in the food production process. One method to inhibit the adhesion of chicken egg white proteins to stainless steel surfaces is to use a citric acid solution [101].
The effect of inhibiting protein adhesion by citric acid involves changing the surface charge of steel from positive to negative in an environment with a pH of 7.4 due to the attachment of dissociated carboxyl groups from the acid. This leads to the repulsion of negatively charged protein molecules such as ovalbumin or ovomucoid [46][101].

This entry is adapted from the peer-reviewed paper 10.3390/molecules29010022

References

  1. Amato, A.; Becci, A.; Beolchini, F. Citric acid bioproduction: The technological innovation change. Crit. Rev. Biotechnol. 2020, 40, 199–212.
  2. Hu, W.; Li, W.J.; Yang, H.Q.; Chen, J. Hong Current strategies and future prospects for enhancing microbial production of citric acid. Appl. Microbiol. Biotechnol. 2019, 103, 201–209.
  3. Citric Acid Market Size, Trends and Forecast 2023–2028. Available online: https://www.imarcgroup.com/citric-acid-manufacturing-plant (accessed on 3 October 2023).
  4. Max, B.; Salgado, J.M.; Rodríguez, N.; Cortés, S.; Converti, A.; Domínguez, J.M. Biotechnological production of citric acid. Braz. J. Microbiol. 2010, 41, 862–875.
  5. Behera, B.C.; Mishra, R.; Mohapatra, S. Microbial citric acid: Production, properties, application, and future perspectives. Food Front. 2021, 2, 62–76.
  6. Wang, B.; Zhao, S.; Tan, F.; Li, H.; Chu, R.; Wang, X.; Sun, H.; Zhang, M. A novel green production process of citric acid on the pilot scale by directly recycling its extraction effluent. J. Clean. Prod. 2020, 277, 124068.
  7. Lende, S.V.; Karemore, H.; Umekar, M.J.; Lende, S.V.; Karemore, H.; Umekar, M.J. Review on production of citric acid by fermentation technology. GSC Biol. Pharm. Sci. 2021, 17, 85–93.
  8. Lambros, M.; Tran, T.H.; Fei, Q.; Nicolaou, M. Citric Acid: A Multifunctional Pharmaceutical Excipient. Pharmaceutics 2022, 14, 972.
  9. Mores, S.; de Vandenberghe, L.P.S.; Magalhães Júnior, A.I.; de Carvalho, J.C.; de Mello, A.F.M.; Pandey, A.; Soccol, C.R. Citric acid bioproduction and downstream processing: Status, opportunities, and challenges. Bioresour. Technol. 2021, 320, 124426.
  10. Chemspider Search and Share Chemistry. Citric Acid |C6H8O7| ChemSpider. Royal Society of Chemistry. Available online: http://www.chemspider.com/Chemical-Structure.305.html?rid=4438fe13-8026-4f48-8dd8-a2625612fc65 (accessed on 22 June 2023).
  11. Apelblat, A. Physicochemical Properties of Inorganic Citrates. In Citric Acid; Springer International Publishing: Berlin/Heidelberg, Germany, 2014; pp. 267–357.
  12. Dziezak, J.D. Encyclopedia of Food Sciences and Nutrition; Academic Press: San Diego, CA, USA, 2003.
  13. Behera, B.C. Citric acid from Aspergillus niger: A comprehensive overview. Crit. Rev. Microbiol. 2020, 46, 727–749.
  14. Fiume, M.; Heldreth, B. On the Safety Assessment of Citric Acid, Inorganic Citrate Salts, and Alkyl Citrate Esters as Used in Cosmetics. 2012. Available online: http://www.cir-safety.org/sites/default/files/citric032012FR.pdf (accessed on 22 June 2014).
  15. Verhoff, F. Ullmann’s Encyclopedia of Industrial Chemistry; Wiley-VCH Verlag GmbH & Co. KGaA: Weinheim, Germany, 2011.
  16. Leśniak, W. Biotechnologia Żywności: Procesy Fermentacji i Biosyntezy; Wydawnictwo Uniwersytetu Ekonomicznego we Wrocławiu: Wrocław, Poland, 2002.
  17. Poerwono, H.; Higashiyama, K.; Kubo, H.; Poernomo, A.T.; Suharjono; Sudiana, I.K.; Indrayanto, G. Citric Acid. Anal. Profiles Drug Subst. Excip. 2001, 28, 1–76.
  18. The National Center for Biotechnology Information. Citric Acid. Pubchem Compound. Available online: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=311 (accessed on 22 June 2023).
  19. Oliveira, M.L.N.; Malagoni, R.A.; Franco, M.R. Solubility of citric acid in water, ethanol, n-propanol and in mixtures of ethanol+water. Fluid Phase Equilib. 2013, 352, 110–113.
  20. Yang, H.; Wang, J. Solubilities of 3-Carboxy-3-hydroxypentanedioic Acid in Ethanol, Butan-1-ol, Water, Acetone, and Methylbenzene. J. Chem. Eng. Data 2011, 56, 1449–1451.
  21. Daneshfar, A.; Baghlani, M.; Sarabi, R.S.; Sahraei, R.; Abassi, S.; Kaviyan, H.; Khezeli, T. Solubility of citric, malonic, and malic acids in different solvents from 303.2 to 333.2K. Fluid Phase Equilib. 2012, 313, 11–15.
  22. Wyrzykowski, D.; Hebanowska, E.; Nowak-Wiczk, G.; Makowski, M.; Chmurzyński, L. Thermal behaviour of citric acid and isomeric aconitic acids. J. Therm. Anal. Calorim. 2010, 104, 731–735.
  23. Leśniak, W. Selekcja wysokoaktywnych szczepów Aspergillus niger dla fermentacji. Pr. Nauk. Akad. Ekon. Wrocławiu. Technol. 1977, 118, 21–37.
  24. Organisation for Economic Co-Operation and Development. SIDS Initial Assessment Report for 11th SIAM. Citric Acid. Available online: http://www.inchem.org/documents/sids/sids/77929.pdf (accessed on 22 June 2022).
  25. Apelblat, A. Dissociation Equilibria in Solutions with Citrate Ions. In Citric Acid; Springer International Publishing: Berlin/Heidelberg, Germany, 2014; pp. 143–212.
  26. West, T.P. Citric Acid Production by Aspergillus niger Using Solid-State Fermentation of Agricultural Processing Coproducts. Appl. Biosci. 2023, 2, 1–13.
  27. Dhillon, G.S.; Brar, S.K.; Kaur, S.; Verma, M. Bioproduction and extraction optimization of citric acid from Aspergillus niger by rotating drum type solid-state bioreactor. Ind. Crops Prod. 2013, 41, 78–84.
  28. Vargas, D.A.; Medina, J.V. Citric Acid: Synthesis, Properties and Applications; Nova Science: New York, NY, USA, 2012; Available online: https://www.novapublishers.com/catalog/product_info.php?products_id=23842 (accessed on 4 April 2017).
  29. Tric acid and salts Handling/Processing. Citric Acid and salts Handling/Processing. Published Online 2015. Available online: https://www.ams.usda.gov/sites/default/files/media/CitricAcid892TR2015.pdf (accessed on 16 April 2017).
  30. Ciriminna, R.; Chavarría-Hernández, N.; Inés Rodríguez Hernández, A.; Pagliaro, M. Pectin: A new perspective from the biorefinery standpoint. Biofuels Bioprod. Biorefining 2015, 9, 368–377.
  31. Ke, S.; Huang, Y.; Decker, E.A.; Hultin, H.O. Impact of citric acid on the tenderness, microstructure and oxidative stability of beef muscle. Meat Sci. 2009, 82, 113–118.
  32. Sammel, L.M.; Claus, J.R. Citric acid and sodium citrate effects on pink color development of cooked ground turkey irradiated pre- and post-cooking. Meat Sci. 2006, 72, 567–573.
  33. Sammel, L.M.; Claus, J.R.; Greaser, M.L.; Richards, M.P. Investigation of mechanisms by which sodium citrate reduces the pink color defect in cooked ground turkey. Meat Sci. 2006, 72, 585–595.
  34. Liu, W.; Zou, L.; Liu, J.; Zhang, Z.; Liu, C.; Liang, R. The effect of citric acid on the activity, thermodynamics and conformation of mushroom polyphenoloxidase. Food Chem. 2013, 140, 289–295.
  35. Queiroz, C.; da Silva, A.J.R.; Lopes, M.L.M.; Fialho, E.; Valente-Mesquita, V.L. Polyphenol oxidase activity, phenolic acid composition and browning in cashew apple (Anacardium occidentale L.) after processing. Food Chem. 2011, 125, 128–132.
  36. Goyeneche, R.; Agüero, M.V.; Roura, S.; Di Scala, K. Application of citric acid and mild heat shock to minimally processed sliced radish: Color evaluation. Postharvest Biol. Technol. 2014, 93, 106–113.
  37. Lücke, F.-K. Encyclopedia of Food Safety; Elsevier: Amsterdam, The Netherlands, 2014; ISBN 9780123786135.
  38. Manolopoulou, E. Effect of Storage Conditions on the Sensory Quality, Colour and Texture of Fresh-Cut Minimally Processed Cabbage with the Addition of Ascorbic Acid, Citric Acid and Calcium Chloride. Food Nutr. Sci. 2011, 2, 956–963.
  39. Zhuang, X.; Zhang, H.; Yang, J.; Qi, H. Preparation of levoglucosan by pyrolysis of cellulose and its citric acid fermentation. Bioresour. Technol. 2001, 79, 63–66.
  40. Goyeneche, R.; Di Scala, K.; Roura, S. Biochemical characterization and thermal inactivation of polyphenol oxidase from radish (Raphanus sativus var. sativus). LWT-Food Sci. Technol. 2013, 54, 57–62.
  41. Rastegar, H.; Shoeibi, S.; Yazdanpanah, H.; Amirahmadi, M.; Khaneghah, A.M.; Campagnollo, F.B.S.; Sant’Ana, A. Removal of aflatoxin B1 by roasting with lemon juice and/or citric acid in contaminated pistachio nuts. Food Control 2017, 71, 279–284.
  42. Ghorpade, V.S.; Yadav, A.V.; Dias, R.J. Citric acid crosslinked β-cyclodextrin/carboxymethylcellulose hydrogel films for controlled delivery of poorly soluble drugs. Carbohydr. Polym. 2017, 164, 339–348.
  43. Olaimat, A.N.; Al-Nabulsi, A.A.; Osaili, T.M.; Al-Holy, M.; Ayyash, M.M.; Mehyar, G.F.; Jaradat, Z.W.; Ghoush, M.A. Survival and inhibition of Staphylococcus aureus in commercial and hydrated tahini using acetic and citric acids. Food Control 2017, 77, 179–186.
  44. Mielcarek, A.; Rodziewicz, J.; Janczukowicz, W.; Dabrowska, D.; Ciesielski, S.; Thornton, A.; Struk-Sokołowska, J. Citric acid application for denitrification process support in biofilm reactor. Chemosphere 2017, 171, 512–519.
  45. Liao, L.; Han, X.; Chen, L.; Ni, L.; Liu, Z.; Zhang, W.; Chen, Q. Comparative characterization of the deamidation of carboxylic acid deamidated wheat gluten by altering the processing conditions. Food Chem. 2016, 210, 520–529.
  46. Hagiwara, T.; Hagihara, S.; Handa, A.; Sasagawa, N.; Kawashima, R.; Sakiyama, T. Pretreatment with citric acid or a mixture of nitric acid and citric acid to suppress egg white protein deposit formation on stainless steel surfaces and to ease its removal during cleaning. Food Control 2015, 53, 35–40.
  47. Ciriminna, R.; Meneguzzo, F.; Delisi, R.; Pagliaro, M. Citric acid: Emerging applications of key biotechnology industrial product. Chem. Cent. J. 2017, 11, 22.
  48. Reddy, N.; Reddy, R.; Jiang, Q. Crosslinking biopolymers for biomedical applications. Trends Biotechnol. 2015, 33, 362–369.
  49. Seligra, P.G.; Medina Jaramillo, C.; Famá, L.; Goyanes, S. Biodegradable and non-retrogradable eco-films based on starch–glycerol with citric acid as crosslinking agent. Carbohydr. Polym. 2016, 138, 66–74.
  50. Sun, D.; Chen, Y.; Tran, R.T.; Xu, S.; Xie, D.; Jia, C.; Wang, Y.; Guo, Y.; Zhang, Z.; Guo, J.; et al. Citric Acid-based Hydroxyapatite Composite Scaffolds Enhance Calvarial Regeneration. Sci. Rep. 2014, 4, 6912.
  51. Alberts, A.H.; Rothenberg, G. Process for Preparing Foamed Polymer. Available online: https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2012052385&recNum=239&docAn=EP2011068076&queryString948=IC/C08G-63/00&maxRec=3738 (accessed on 8 April 2017).
  52. Olsson, E.; Hedenqvist, M.S.; Johansson, C.; Järnström, L. Influence of citric acid and curing on moisture sorption, diffusion and permeability of starch films. Carbohydr. Polym. 2013, 94, 765–772.
  53. Garcia, P.S.; Grossmann, M.V.E.; Yamashita, F.; Mali, S.; Dall’Antonia, L.H.; Barreto, W.J. Citric acid as multifunctional agent in blowing films of starch/PBAT. Quim. Nova 2011, 34, 1507–1510.
  54. Olsson, E.; Menzel, C.; Johansson, C.; Andersson, R.; Koch, K.; Järnström, L. The effect of pH on hydrolysis, cross-linking and barrier properties of starch barriers containing citric acid. Carbohydr. Polym. 2013, 98, 1505–1513.
  55. Menzel, C.; Olsson, E.; Plivelic, T.S.; Andersson, R.; Johansson, C.; Kuktaite, R.; Järnström, L.; Koch, K. Molecular structure of citric acid cross-linked starch films. Carbohydr. Polym. 2013, 96, 270–276.
  56. Shi, R.; Zhang, Z.; Liu, Q.; Han, Y.; Zhang, L.; Chen, D.; Tian, W. Characterization of citric acid/glycerol co-plasticized thermoplastic starch prepared by melt blending. Carbohydr. Polym. 2007, 69, 748–755.
  57. Ma, X.; Chang, P.R.; Yu, J.; Stumborg, M. Properties of biodegradable citric acid-modified granular starch/thermoplastic pea starch composites. Carbohydr. Polym. 2009, 75, 1–8.
  58. Fajd, E.; Marton, G. Starch Citrate as an Ion Exchange Material–Preparation and Investigation. Hung. J. Ind. Chem. 2004, 32.
  59. Wing, R.E. Starch Citrate: Preparation and Ion Exchange Properties. Starch-Starke 1996, 48, 275–279.
  60. Ghanbarzadeh, B.; Almasi, H.; Entezami, A.A. Improving the barrier and mechanical properties of corn starch-based edible films: Effect of citric acid and carboxymethyl cellulose. Ind. Crops Prod. 2011, 33, 229–235.
  61. Reddy, N.; Yang, Y. Citric acid cross-linking of starch films. Food Chem. 2010, 118, 702–711.
  62. Shi, R.; Bi, J.; Zhang, Z.; Zhu, A.; Chen, D.; Zhou, X.; Zhang, L.; Tian, W. The effect of citric acid on the structural properties and cytotoxicity of the polyvinyl alcohol/starch films when molding at high temperature. Carbohydr. Polym. 2008, 74, 763–770.
  63. Garcia, P.S.; Grossmann, M.V.E.; Shirai, M.A.; Lazaretti, M.M.; Yamashita, F.; Muller, C.M.O.; Mali, S. Improving action of citric acid as compatibiliser in starch/polyester blown films. Ind. Crops Prod. 2014, 52, 305–312.
  64. Abdillahi, H.; Chabrat, E.; Rouilly, A.; Rigal, L. Influence of citric acid on thermoplastic wheat flour/poly(lactic acid) blends. II. Barrier properties and water vapor sorption isotherms. Ind. Crops Prod. 2013, 50, 104–111.
  65. Chabrat, E.; Abdillahi, H.; Rouilly, A.; Rigal, L. Influence of citric acid and water on thermoplastic wheat flour/poly(lactic acid) blends. I: Thermal, mechanical and morphological properties. Ind. Crops Prod. 2012, 37, 238–246.
  66. Ghorpade, V.S.; Yadav, A.V.; Dias, R.J. Citric acid crosslinked cyclodextrin/hydroxypropylmethylcellulose hydrogel films for hydrophobic drug delivery. Int. J. Biol. Macromol. 2016, 93, 75–86.
  67. Hu, Y.; Liang, P.; Wang, Z.; Zhu, H.; Liu, Q. Developing amino acid-citric acid-based deep eutectic solvent for food applications: Preparation, characterization, antibacterial activity, biosafety, and formation mechanism exploration. Sustain. Chem. Pharm. 2023, 36, 101317.
  68. Hu, Y.; Wang, Z.; Liang, P.; Zhu, H.; Liu, Q. Exploring the molecular mechanisms of isoliquiritin extraction using choline chloride-citric acid deep eutectic solvents. Sustain. Chem. Pharm. 2023, 33, 101099.
  69. Dai, Y.; van Spronsen, J.; Witkamp, G.J.; Verpoorte, R.; Choi, Y.H. Natural deep eutectic solvents as new potential media for green technology. Anal. Chim. Acta 2013, 766, 61–68.
  70. Qin, H.; Hu, X.; Wang, J.; Cheng, H.; Chen, L.; Qi, Z. Overview of acidic deep eutectic solvents on synthesis, properties and applications. Green Energy Environ. 2020, 5, 8–21.
  71. Zaib, Q.; Eckelman, M.J.; Yang, Y.; Kyung, D. Are deep eutectic solvents really green?: A life-cycle perspective. Green Chem. 2022, 24, 7924–7930.
  72. Liu, Y.; Friesen, J.B.; McAlpine, J.B.; Lankin, D.C.; Chen, S.N.; Pauli, G.F. Natural Deep Eutectic Solvents: Properties, Applications, and Perspectives. J. Nat. Prod. 2018, 81, 679–690.
  73. Omar, K.A.; Sadeghi, R. Physicochemical properties of deep eutectic solvents: A review. J. Mol. Liq. 2022, 360, 119524.
  74. Koigerova, A.; Gosteva, A.; Samarov, A.; Tsvetov, N. Deep Eutectic Solvents Based on Carboxylic Acids and Glycerol or Propylene Glycol as Green Media for Extraction of Bioactive Substances from Chamaenerion angustifolium (L.) Scop. Molecules 2023, 28, 6978.
  75. González-Fandos, E.; Herrera, B.; Maya, N. Efficacy of citric acid against Listeria monocytogenes attached to poultry skin during refrigerated storage. Int. J. Food Sci. Technol. 2009, 44, 262–268.
  76. Koutsoumanis, K.; Skandamis, P. 18—New research on organic acids and pathogen behaviour. In Advances in Microbial Food Safety; Elsevier: Amsterdam, The Netherlands, 2013; pp. 355–384. ISBN 9780857094384.
  77. Schmidt, S.E.; Taylor, T.M.; Davidson, P.M. Chemical Preservatives and Natural Antimicrobial Compounds. In Food Microbiology; American Society of Microbiology: Washington, DC, USA, 2013; pp. 765–801.
  78. Al-Nabulsi, A.A.; Olaimat, A.N.; Osaili, T.M.; Shaker, R.R.; Zein Elabedeen, N.; Jaradat, Z.W.; Abushelaibi, A.; Holley, R.A. Use of acetic and citric acids to control Salmonella Typhimurium in tahini (sesame paste). Food Microbiol. 2014, 42, 102–108.
  79. Choi, M.J.; Kim, S.A.; Lee, N.Y.; Rhee, M.S. New decontamination method based on caprylic acid in combination with citric acid or vanillin for eliminating Cronobacter sakazakii and Salmonella enterica serovar Typhimurium in reconstituted infant formula. Int. J. Food Microbiol. 2013, 166, 499–507.
  80. Mahmoud, B.S.M. The efficacy of grape seed extract, citric acid and lactic acid on the inactivation of Vibrio parahaemolyticus in shucked oysters. Food Control 2014, 41, 13–16.
  81. García-Soto, B.; Fernández-No, I.C.; Barros-Velázquez, J.; Aubourg, S.P. Use of citric and lactic acids in ice to enhance quality of two fish species during on-board chilled storage. Int. J. Refrig. 2014, 40, 390–397.
  82. Bermúdez-Aguirre, D.; Barbosa-Cánovas, G.V. Disinfection of selected vegetables under nonthermal treatments: Chlorine, acid citric, ultraviolet light and ozone. Food Control 2013, 29, 82–90.
  83. Alakomi, H.-L.; Puupponen-Pimiä, R.; Aura, A.-M.; Helander, I.M.; Nohynek, L.; Oksman-Caldentey, K.-M.; Saarela, M. Weakening of salmonella with selected microbial metabolites of berry-derived phenolic compounds and organic acids. J. Agric. Food Chem. 2007, 55, 3905–3912.
  84. Mani-López, E.; García, H.S.; López-Malo, A. Organic acids as antimicrobials to control Salmonella in meat and poultry products. Food Res. Int. 2012, 45, 713–721.
  85. Doores, S. Organic acid. In Antimicrobials in Foods, 3rd ed.; Davidson, P.M.B.A.L., Ed.; Marcel Dekker Inc: New York, NY, USA, 2005; pp. 95–136.
  86. Radkowski, M.; Mikołajczyk, A. Wplyw kwasu cytrynowego na unieszkodliwianie paleczek Salmonella w miesie drobiowym. Med. Weter 2009, 65, 840–843.
  87. Seo, S.; Jung, D.; Wang, X.; Seo, D.J.; Lee, M.H.; Lee, B.-H.; Choi, C. Combined effect of lactic acid bacteria and citric acid on Escherichia coli O157:H7 and Salmonella Typhimurium. Food Sci. Biotechnol. 2013, 22, 1171–1174.
  88. Rey, M.S.; García-Soto, B.; Fuertes-Gamundi, J.R.; Aubourg, S.; Barros-Velázquez, J. Effect of a natural organic acid-icing system on the microbiological quality of commercially relevant chilled fish species. LWT-Food Sci. Technol. 2012, 46, 217–223.
  89. Chen, Y.; Nielsen, J. Biobased organic acids production by metabolically engineered microorganisms. Curr. Opin. Biotechnol. 2016, 37, 165–172.
  90. Tamblyn, K.C.; Conner, D.E. Bactericidal Activity of Organic Acids against Salmonella typhimurium Attached to Broiler Chicken Skin. J. Food Prot. 1997, 6, 610–737.
  91. Qiu, C.; Sun, W.; Cui, C.; Zhao, M. Effect of citric acid deamidation on in vitro digestibility and antioxidant properties of wheat gluten. Food Chem. 2013, 141, 2772–2778.
  92. He, W.; Zhao, W.; Yang, R. Effects of wheat gluten modified by deamidation-heating with three different acids on the microstructure of model oil-in-water emulsion and rheological–physical property of ice cream. Food Hydrocoll. 2019, 87, 679–690.
  93. Qiu, C.; Sun, W.; Zhao, Q.; Cui, C.; Zhao, M. Emulsifying and surface properties of citric acid deamidated wheat gliadin. J. Cereal Sci. 2013, 58, 68–75.
  94. Liao, L.; Liu, T.; Zhao, M.; Zhao, H.; Cui, C. Aggregation behavior of wheat gluten during carboxylic acid deamidation upon hydrothermal treatment. J. Cereal Sci. 2011, 54, 129–136.
  95. Liao, L.; Luo, Y.; Zhao, M.; Wang, Q. Preparation and characterization of succinic acid deamidated wheat gluten microspheres for encapsulation of fish oil. Colloids Surf. B. Biointerfaces 2012, 92, 305–314.
  96. Dai, Y.; Zhong, W.; Hu, X.; Ji, X.; Sun, Q.; Wang, R.; Yu, D.; Wu, F.; Wang, L. Conformational and functional changes from deamidation of wheat gluten with electrochemical treatment. J. Sci. Food Agric. 2023, 103, 5677–5686.
  97. Canteri-Schemin, M.H.; Fertonani, H.C.R.; Waszczynskyj, N.; Wosiacki, G. Extraction of pectin from apple pomace. Braz. Arch. Biol. Technol. 2005, 48, 259–266.
  98. Yapo, B.M. Lemon juice improves the extractability and quality characteristics of pectin from yellow passion fruit by-product as compared with commercial citric acid extractant. Bioresour. Technol. 2009, 100, 3147–3151.
  99. Kurita, O.; Fujiwara, T.; Yamazaki, E. Characterization of the pectin extracted from citrus peel in the presence of citric acid. Carbohydr. Polym. 2008, 74, 725–730.
  100. Vriesmann, L.C.; Teófilo, R.F.; Lúcia de Oliveira Petkowicz, C. Extraction and characterization of pectin from cacao pod husks (Theobroma cacao L.) with citric acid. LWT-Food Sci. Technol. 2012, 49, 108–116.
  101. Sakiyama, T.; Sato, K.; Tsuda, S.; Sugiyama, H.; Hagiwara, T. Citric acid pretreatment for suppressing adhesion of major egg allergens to a stainless steel surface. Food Control 2013, 32, 702–706.
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