PHT1-5 in Allotetraploid Brassica Napus: Comparison
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Phosphate (Pi) transporters play critical roles in Pi acquisition and homeostasis. However, currently little is known about these genes in oil crops. In this study, we aimed to characterize the five Pi transporter gene families (PHT1-5) in allotetraploid

Brassica napus

. We identified and characterized 81 putative PHT genes in

B. napus

(

BnaPHTs

), including 45 genes in PHT1 family (

BnaPHT1s

), four

BnaPHT2s

, 10

BnaPHT3s

, 13

BnaPHT4s

and nine

BnaPHT5s

. Phylogenetic analyses showed that the largest PHT1 family could be divided into two groups (Group I and II), while PHT4 may be classified into five, Groups I-V. Gene structure analysis revealed that the exon-intron pattern was conservative within the same family or group. The sequence characteristics of these five families were quite different, which may contribute to their functional divergence. Transcription factor (TF) binding network analyses identified many potential TF binding sites in the promoter regions of candidates, implying their possible regulating patterns. Collinearity analysis demonstrated that most

BnaPHTs

were derived from an allopolyploidization event (~40.7%) between

Brassica rapa

and

Brassica oleracea 

ancestors, and small-scale segmental duplication events (~39.5%) in the descendant. RNA-Seq analyses proved that many

BnaPHTs

were preferentially expressed in leaf and flower tissues. The expression profiles of most colinearity-pairs in

B. napus

are highly correlated, implying functional redundancy, while a few pairs may have undergone neo-functionalization or sub-functionalization during evolution. The expression levels of many

BnaPHTs

tend to be up-regulated by diifferent hormones inductions, especially for IAA, ABA and 6-BA treatments. qRT-PCR assay demonstrated that six

BnaPHT1s

(

BnaPHT1.11

,

BnaPHT1.14

,

BnaPHT1.20

,

BnaPHT1.35

,

BnaPHT1.41

,

BnaPHT1.44

) were significantly up-regulated under low- and/or rich- Pi conditions in

B. napus

roots. 

  • Brassica napusL.
  • PHT gene family
  • phylogenetic analysis
  • expression analysis
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References

  1. Plos Genetics Staff; The Plos Genetics Staff; Correction: Fungal Infection Induces Sex-Specific Transcriptional Changes and Alters Sexual Dimorphism in the Dioecious Plant Silene latifolia.. PLOS Genetics 2015, 11, e1005662, 10.1371/journal.pgen.1005662.
  2. Stephen R. Mudge; Anne L. Rae; Eugene Diatloff; Frank W. Smith; Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis.. The Plant Journal 2002, 31, 341-353, 10.1046/j.1365-313X.2002.01356.x.
  3. Fang Liu; Xiao-Jian Chang; Ying Ye; Weibo Xie; Ping Wu; Xingming Lian; Comprehensive Sequence and Whole-Life-Cycle Expression Profile Analysis of the Phosphate Transporter Gene Family in Rice. Molecular Plant 2011, 4, 1105-1122, 10.1093/mp/ssr058.
  4. Chunxia Zhang; Sen Meng; Mingjun Li; Zhong Zhao; Genomic Identification and Expression Analysis of the Phosphate Transporter Gene Family in Poplar. Frontiers in Plant Science 2016, 7, 798, 10.3389/fpls.2016.01398.
  5. Tingting Sun; Mingjun Li; Yun Shao; Lingyan Yu; Fengwang Ma; Comprehensive Genomic Identification and Expression Analysis of the Phosphate Transporter (PHT) Gene Family in Apple. Frontiers in Plant Science 2017, 8, 225, 10.3389/fpls.2017.00426.
  6. Duoliya Wang; Sulian Lv; Ping Jiang; Yinxin Li; Roles, Regulation, and Agricultural Application of Plant Phosphate Transporters. Frontiers in Plant Science 2017, 8, , 10.3389/fpls.2017.00817.
  7. R. A. Narang; Analysis of Phosphate Acquisition Efficiency in Different Arabidopsis Accessions. Plant Physiology 2000, 124, 1786-1799, 10.1104/pp.124.4.1786.
  8. Pierre Daram; Silvia Brunner; Christine Rausch; Cyrill Steiner; Nikolaus Amrhein; Marcel Bucher; Pht2;1 Encodes a Low-Affinity Phosphate Transporter from Arabidopsis. The Plant Cell 1999, 11, 2153, 10.2307/3871016.
  9. Ballachanda N. Devaiah; Ramaiah Madhuvanthi; Athikkattuvalasu S. Karthikeyan; Kashchandra G. Raghothama; Phosphate Starvation Responses and Gibberellic Acid Biosynthesis Are Regulated by the MYB62 Transcription Factor in Arabidopsis. Molecular Plant 2008, 2, 43-58, 10.1093/mp/ssn081.
  10. Hui Wang; Qian Xu; You-Han Kong; Yun Chen; Jun-Ye Duan; Wei-Hua Wu; Yi-Fang Chen; Arabidopsis WRKY45 transcription factor activates PHOSPHATE TRANSPORTER1;1 expression in response to phosphate starvation.. Plant Physiology 2014, 164, 2020-9, 10.1104/pp.113.235077.
  11. Karin Ljung; Auxin metabolism and homeostasis during plant development. Development 2013, 140, 943-950, 10.1242/dev.086363.
  12. Peter Hedden; Gibberellin Metabolism and Its Regulation. Journal of Plant Growth Regulation 2001, 20, 317-318, 10.1007/s003440010039.
  13. Y Deng; L Zhao; L Zhang; [Effects of sand and hormone treatment on seed germination of Bupleurum chinese DC. and B. falcatum L].. China Journal of Chinese Materia Medica 1996, 21, , null.
  14. Ngwon Baek; Hyun Jin Chun; Dae-Jin Yun; Min Chul Kim; Cross-talk between Phosphate Starvation and Other Environmental Stress Signaling Pathways in Plants. Molecules and Cells 2017, 40, 697-705, 10.14348/molcells.2017.0192.
  15. T. R. Bates; J. P. Lynch; Stimulation of root hair elongation in Arabidopsis thaliana by low phosphorus availability. Plant, Cell & Environment 1996, 19, 529-538, 10.1111/j.1365-3040.1996.tb00386.x.
  16. Jonathan Lynch; Kathleen M Brown; Ethylene and plant responses to nutritional stress. Physiologia Plantarum 1997, 100, 613-619, 10.1034/j.1399-3054.1997.1000324.x.
  17. Glena A. Gilbert; J. Diane Knight; Deborah L. Allan; Carroll P. Vance; Proteoid Root Development of Phosphorus Deficient Lupin is Mimicked by Auxin and Phosphonate. Annals Of Botany 2000, 85, 921-928, 10.1006/anbo.2000.1133.
  18. Hongfang Jia; Yongxia Yang; Huifang Shao; Songtao Zhang; Lizhi Wang; Hong Cui; Guohua Xu; OsPht1;8, a phosphate transporter, is involved in auxin and phosphate starvation response in rice. Journal of Experimental Botany 2017, 68, 5057-5068, 10.1093/jxb/erx317.
  19. Yu Li; Xue Wang; Hao Zhang; Sheliang Wang; Xiangsheng Ye; Lei Shi; Fangsen Xu; Guangda Ding; Molecular identification of the phosphate transporter family 1 (PHT1) genes and their expression profiles in response to phosphorus deprivation and other abiotic stresses in Brassica napus.. PLOS ONE 2019, 14, e0220374, 10.1371/journal.pone.0220374.
  20. Martin, A.C.; del Pozo, J.C.; Iglesias, J.; Rubio, V.; Solano, R.; de la Pena, A.; Leyva, A.; Paz-Ares, J. Influence of cytokininson the expression of phosphate starvation responsive genes in Arabidopsis. Plant J. 2000, 24, 559–567.
  21. Heungsop Shin; Hwa-Soo Shin; Gary R. Dewbre; Maria J. Harrison; Phosphate transport inArabidopsis: Pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low- and high-phosphate environments. The Plant Journal 2004, 39, 629-642, 10.1111/j.1365-313x.2004.02161.x.
  22. Shubin Sun; Mian Gu; Yue Cao; Xinpeng Huang; Xiao Zhang; Penghui Ai; Jianning Zhao; Xiaorong Fan; Guohua Xu; A constitutive expressed phosphate transporter, OsPht1;1, modulates phosphate uptake and translocation in phosphate-replete rice.. Plant Physiology 2012, 159, 1571-81, 10.1104/pp.112.196345.
  23. Estelle Remy; T. R. Cabrito; Rita A Batista; Miguel C Teixeira; Isabel Sá-Correia; Paula Duque; The Pht1;9 and Pht1;8 transporters mediate inorganic phosphate acquisition by the Arabidopsis thaliana root during phosphorus starvation. New Phytologist 2012, 195, 356-371, 10.1111/j.1469-8137.2012.04167.x.
  24. Daniel P. Schachtman; Ryoung Shin; Nutrient Sensing and Signaling: NPKS. Annual Review of Plant Biology 2007, 58, 47-69, 10.1146/annurev.arplant.58.032806.103750.
  25. Sally E. Smith; Iver Jakobsen; Mette Grønlund; F. Andrew Smith; Roles of Arbuscular Mycorrhizas in Plant Phosphorus Nutrition: Interactions between Pathways of Phosphorus Uptake in Arbuscular Mycorrhizal Roots Have Important Implications for Understanding and Manipulating Plant Phosphorus Acquisition1. Plant Physiology 2011, 156, 1050-1057, 10.1104/pp.111.174581.
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