1. Radiative Transfer Equation

The RTE is a differential equation describing radiance [math]\displaystyle{ L(\vec{r},\hat{s},t) }[/math]. It can be derived via conservation of energy. Briefly, the RTE states that a beam of light loses energy through divergence and extinction (including both absorption and scattering away from the beam) and gains energy from light sources in the medium and scattering directed towards the beam. Coherence, polarization and non-linearity are neglected. Optical properties such as refractive index [math]\displaystyle{ n }[/math], absorption coefficient μ_a, scattering coefficient μ_s, and scattering anisotropy [math]\displaystyle{ g }[/math] are taken as time-invariant but may vary spatially. Scattering is assumed to be elastic. The RTE (Boltzmann equation) is thus written as:

[math]\displaystyle{ \frac{\partial L(\vec{r},\hat{s},t)/c}{\partial t} = -\hat{s}\cdot \nabla L(\vec{r},\hat{s},t)-\mu_tL(\vec{r},\hat{s},t)+\mu_s\int_{4\pi}L(\vec{r},\hat{s}',t)P(\hat{s}',\hat{s})d\Omega' + S(\vec{r},\hat{s},t) }[/math]

where

• [math]\displaystyle{ c }[/math] is the speed of light in the tissue, as determined by the relative refractive index
• μ_t[math]\displaystyle{ = }[/math]μ_a+μ_s is the extinction coefficient
• [math]\displaystyle{ P(\hat{s}',\hat{s}) }[/math] is the phase function, representing the probability of light with propagation direction [math]\displaystyle{ \hat{s}' }[/math] being scattered into solid angle [math]\displaystyle{ d\Omega }[/math] around [math]\displaystyle{ \hat{s} }[/math]. In most cases, the phase function depends only on the angle between the scattered [math]\displaystyle{ \hat{s}' }[/math] and incident [math]\displaystyle{ \hat{s} }[/math] directions, i.e. [math]\displaystyle{ P(\hat{s}',\hat{s})=P(\hat{s}'\cdot\hat{s}) }[/math]. The scattering anisotropy can be expressed as [math]\displaystyle{ g=\int_{4\pi}(\hat{s}'\cdot\hat{s})P(\hat{s}'\cdot\hat{s})d\Omega }[/math]
• [math]\displaystyle{ S(\vec{r},\hat{s},t) }[/math] describes the light source.

2. Diffusion Theory

2.1. Assumptions

In the RTE, six different independent variables define the radiance at any spatial and temporal point ([math]\displaystyle{ x }[/math], [math]\displaystyle{ y }[/math], and [math]\displaystyle{ z }[/math] from [math]\displaystyle{ \vec{r} }[/math], polar angle [math]\displaystyle{ \theta }[/math] and azimuthal angle [math]\displaystyle{ \phi }[/math] from [math]\displaystyle{ \hat{s} }[/math], and [math]\displaystyle{ t }[/math]). By making appropriate assumptions about the behavior of photons in a scattering medium, the number of independent variables can be reduced. These assumptions lead to the diffusion theory (and diffusion equation) for photon transport. Two assumptions permit the application of diffusion theory to the RTE:

• Relative to scattering events, there are very few absorption events. Likewise, after numerous scattering events, few absorption events will occur and the radiance will become nearly isotropic. This assumption is sometimes called directional broadening.
• In a primarily scattering medium, the time for substantial current density change is much longer than the time to traverse one transport mean free path. Thus, over one transport mean free path, the fractional change in current density is much less than unity. This property is sometimes called temporal broadening.

It should be noted that both of these assumptions require a high-albedo (predominantly scattering) medium.

2.2. The RTE in the Diffusion Approximation

Radiance can be expanded on a basis set of spherical harmonics [math]\displaystyle{ Y }[/math]_{n, m}. In diffusion theory, radiance is taken to be largely isotropic, so only the isotropic and first-order anisotropic terms are used: [math]\displaystyle{ L(\vec{r},\hat{s},t) \approx\ \sum_{n=0}^{1} \sum_{m=-n}^{n}L_{n,m}(\vec{r},t)Y_{n,m}(\hat{s}) }[/math] where [math]\displaystyle{ L }[/math]_{n, m} are the expansion coefficients. Radiance is expressed with 4 terms; one for n = 0 (the isotropic term) and 3 terms for n = 1 (the anisotropic terms). Using properties of spherical harmonics and the definitions of fluence rate [math]\displaystyle{ \Phi(\vec{r},t) }[/math] and current density [math]\displaystyle{ \vec{J}(\vec{r},t) }[/math], the isotropic and anisotropic terms can respectively be expressed as follows:

• [math]\displaystyle{ L_{0,0}(\vec{r},t)Y_{0,0}(\hat{s})=\frac{\Phi(\vec{r},t)}{4\pi} }[/math]
• [math]\displaystyle{ \sum_{m=-1}^{1}L_{1,m}(\vec{r},t)Y_{1,m}(\hat{s})=\frac{3}{4\pi}\vec{J}(\vec{r},t)\cdot \hat{s} }[/math]

Hence we can approximate radiance as

[math]\displaystyle{ L(\vec{r},\hat{s},t)=\frac{1}{4\pi}\Phi(\vec{r},t)+\frac{3}{4\pi}\vec{J}(\vec{r},t)\cdot \hat{s} }[/math]

Substituting the above expression for radiance, the RTE can be respectively rewritten in scalar and vector forms as follows (The scattering term of the RTE is integrated over the complete [math]\displaystyle{ 4\pi }[/math] solid angle. For the vector form, the RTE is multiplied by direction [math]\displaystyle{ \hat{s} }[/math] before evaluation.):

[math]\displaystyle{ \frac{\partial \Phi(\vec{r},t)}{c\partial t} + \mu_a\Phi(\vec{r},t) + \nabla \cdot \vec{J}(\vec{r},t) = S(\vec{r},t) }[/math]

[math]\displaystyle{ \frac{\partial \vec{J}(\vec{r},t)}{c\partial t} + (\mu_a+\mu_s')\vec{J}(\vec{r},t) + \frac{1}{3}\nabla \Phi(\vec{r},t) = 0 }[/math]

The diffusion approximation is limited to systems where reduced scattering coefficients are much larger than their absorption coefficients and having a minimum layer thickness of the order of a few transport mean free path.

2.3. The Diffusion Equation

Using the second assumption of diffusion theory, we note that the fractional change in current density [math]\displaystyle{ \vec{J}(\vec{r},t) }[/math] over one transport mean free path is negligible. The vector representation of the diffusion theory RTE reduces to Fick's law [math]\displaystyle{ \vec{J}(\vec{r},t)=\frac{-\nabla \Phi(\vec{r},t)}{3(\mu_a+\mu_s')} }[/math], which defines current density in terms of the gradient of fluence rate. Substituting Fick's law into the scalar representation of the RTE gives the diffusion equation:

[math]\displaystyle{ \frac{1}{c}\frac{\partial \Phi(\vec{r},t)}{\partial t} + \mu_a\Phi(\vec{r},t) - \nabla \cdot [D\nabla\Phi(\vec{r},t)] = S(\vec{r},t) }[/math]

[math]\displaystyle{ D=\frac{1}{3(\mu_a+\mu_s')} }[/math] is the diffusion coefficient and μ'_s[math]\displaystyle{ =(1-g) }[/math]μ_s is the reduced scattering coefficient.

Notably, there is no explicit dependence on the scattering coefficient in the diffusion equation. Instead, only the reduced scattering coefficient appears in the expression for [math]\displaystyle{ D }[/math]. This leads to an important relationship; diffusion is unaffected if the anisotropy of the scattering medium is changed while the reduced scattering coefficient stays constant.

3. Solutions to the Diffusion Equation

For various configurations of boundaries (e.g. layers of tissue) and light sources, the diffusion equation may be solved by applying appropriate boundary conditions and defining the source term [math]\displaystyle{ S(\vec{r},t) }[/math] as the situation demands.

3.1. Point Sources in Infinite Homogeneous Media

A solution to the diffusion equation for the simple case of a short-pulsed point source in an infinite homogeneous medium is presented in this section. The source term in the diffusion equation becomes [math]\displaystyle{ S(\vec{r},t, \vec{r'},t')=\delta(\vec{r}-\vec{r'})\delta(t-t') }[/math], where [math]\displaystyle{ \vec{r} }[/math] is the position at which fluence rate is measured and [math]\displaystyle{ \vec{r'} }[/math] is the position of the source. The pulse peaks at time [math]\displaystyle{ t' }[/math]. The diffusion equation is solved for fluence rate to yield

[math]\displaystyle{ \Phi(\vec{r},t;\vec{r'},t)=\frac{c}{[4\pi Dc(t-t')]^{3/2}}\exp\left[-\frac{\mid \vec{r}-\vec{r'} \mid ^2}{4Dc(t-t')}\right]\exp[-\mu_ac(t-t')] }[/math]

The term [math]\displaystyle{ \exp\left[-\mu_ac(t-t')\right] }[/math] represents the exponential decay in fluence rate due to absorption in accordance with Beer's law. The other terms represent broadening due to scattering. Given the above solution, an arbitrary source can be characterized as a superposition of short-pulsed point sources. Taking time variation out of the diffusion equation gives the following for a time-independent point source [math]\displaystyle{ S(\vec{r})=\delta(\vec{r}) }[/math]:

[math]\displaystyle{ \Phi(\vec{r})=\frac{1}{4\pi Dr}\exp(-\mu_{\mathrm{eff}}r) }[/math]

[math]\displaystyle{ \mu_{\mathrm{eff}}=\sqrt{\frac{\mu_a}{D}} }[/math] is the effective attenuation coefficient and indicates the rate of spatial decay in fluence.

3.2. Boundary Conditions

Fluence rate at a boundary

Consideration of boundary conditions permits use of the diffusion equation to characterize light propagation in media of limited size (where interfaces between the medium and the ambient environment must be considered). To begin to address a boundary, one can consider what happens when photons in the medium reach a boundary (i.e. a surface). The direction-integrated radiance at the boundary and directed into the medium is equal to the direction-integrated radiance at the boundary and directed out of the medium multiplied by reflectance [math]\displaystyle{ R_F }[/math]:

[math]\displaystyle{ \int_{\hat{s}\cdot \hat{n}\lt 0}L(\vec{r},\hat{s},t)\hat{s}\cdot \hat{n} d\Omega=\int_{\hat{s}\cdot \hat{n}\gt 0}R_F(\hat{s}\cdot \hat{n})L(\vec{r},\hat{s},t)\hat{s}\cdot \hat{n}d\Omega }[/math]

where [math]\displaystyle{ \hat{n} }[/math] is normal to and pointing away from the boundary. The diffusion approximation gives an expression for radiance [math]\displaystyle{ L }[/math] in terms of fluence rate [math]\displaystyle{ \Phi }[/math] and current density [math]\displaystyle{ \vec{J} }[/math]. Evaluating the above integrals after substitution gives:^[1]

Figure 21: The steps in representing a pencil beam incident on a semi-infinite anisotropically scattering medium as two isotropic point sources in an infinite medium. https://handwiki.org/wiki/index.php?curid=1510613

[math]\displaystyle{ \frac{\Phi(\vec{r},t)}{4}+\vec{J}(\vec{r},t)\cdot \frac{\hat{n}}{2}=R_{\Phi}\frac{\Phi(\vec{r},t)}{4}-R_{J}\vec{J}(\vec{r},t)\cdot \frac{\hat{n}}{2} }[/math]

• [math]\displaystyle{ R_{\Phi}=\int_{0}^{\pi/2}2\sin \theta \cos \theta R_F(\cos \theta)d\theta }[/math]
• [math]\displaystyle{ R_{J}=\int_{0}^{\pi/2}3\sin \theta (\cos \theta)^2 R_F(\cos \theta)d\theta }[/math]

Substituting Fick's law ([math]\displaystyle{ \vec{J}(\vec{r},t)=-D\nabla \Phi(\vec{r},t) }[/math]) gives, at a distance from the boundary z=0,^[1]

[math]\displaystyle{ \Phi(\vec{r},t)=A_z\frac{\partial \Phi(\vec{r},t)}{\partial z} }[/math]

• [math]\displaystyle{ A_z=2D\frac{1+R_{\mathrm{eff}}}{1-R_{\mathrm{eff}}} }[/math]
• [math]\displaystyle{ R_{\mathrm{eff}}=\frac{R_{\Phi}+R_{J}}{2-R_{\Phi}+R_J} }[/math]

The extrapolated boundary

It is desirable to identify a zero-fluence boundary. However, the fluence rate [math]\displaystyle{ \Phi(z=0, t) }[/math] at a physical boundary is, in general, not zero. An extrapolated boundary, at [math]\displaystyle{ z }[/math]_b for which fluence rate is zero, can be determined to establish image sources. Using a first order Taylor series approximation,