Glycated hemoglobin (HbA1c) is the gold standard for measuring glucose levels in the diagnosis of diabetes due to the excellent stability and reliability of this biomarker. HbA1c is a stable glycated protein formed by the reaction of glucose with hemoglobin (Hb) in red blood cells, which reflects average glucose levels over a period of two to three months without suffering from the disturbance of the outside environment. A number of simple, high-efficiency, and sensitive electrochemical sensors have been developed for the detection of HbA1c. Indirect type electrochemical HbA1c sensors work based on the measurement of fructosyl valine/fructosyl valine histidine (FV or FVH), which is a form of enzymatic determination. According to the type of enzymes, indirect sensors are divided into fructosyl amino acid oxidase (FAO) type, fructosyl peptide oxidase (FPOX) type, and molecularly imprinted catalyst (MIC) type sensors. These enzymes usually need to be processed with nanotechnology or imprinting technology to immobilize them on the electrode surface.