Squamous Cell Carcinoma | Encyclopedia MDPI

Squamous Cell Carcinoma: Comparison

Please note this is a comparison between Version 1 by Jing Wang and Version 2 by Rita Xu.

More than 90% of all head and neck cancers (HNCs) are head and neck squamous cell carcinomas (HNSCCs) arising from the mucosal surfaces of the upper aerodigestive tract. HNSCCs are the the sixth most prevalent cancer worldwide, and are often associated with either carcinogens, such as alcohol and tobacco use, or oncogenic human papillomavirus (HPV) infection. HNSCCs have been found to be diverse with a high rate of genetic heterogeneity, resulting in hyper-activation of oncogenes (e.g.,

PIK3CA

and

HRAS

) and loss-of-function mutations in tumor suppressor genes (e.g.,

TP53

CASP8

, and

NOTCH1

). HNSCC cohorts from The Cancer Genome Atlas (TCGA) RNA-seq data and clinical data show patients with

PIK3CA

alterations, including amplification and gain, also have a higher chance of harboring

TP53

mutations. In addition, these patients bearing both mutations have a significantly worse 10-year survival prognosis compared with their wildtype cohort counterparts.

HNSCC translational research
HNSCC preclinical models
Head and neck squamous cell carcinoma
PIK3CA/TP53
TCGA
gene mutations
deletions
amplifications
Squamous Cell Carcinoma
SCC

1. Introduction

Head and neck cancers (HNC) are a heterogeneous group of tumors arising from the mucosal surfaces of the upper aerodigestive tract [1]. Collectively, HNC is the sixth most prevalent cancer worldwide [1]. Some 90% of all HNCs are head and neck squamous cell carcinomas (HNSCCs) and HNSCCs are often associated with either carcinogens, such as alcohol and tobacco use, or oncogenic human papillomavirus (HPV) infection ^[2][3][2,3], thereby categorized as HPV(−) or HPV(+) HNSCCs. HNSCCs have been found to be diverse with a high rate of genetic heterogeneity, resulting in hyper-activation of oncogenes (e.g., PIK3CA and HRAS) and loss-of-function mutations in tumor suppressor genes (e.g., TP53, CASP8, and NOTCH1) ^[4][5][4,5]. Phosphoinositide 3-kinase (PI3K) is a frequently deregulated pathway in HNSCCs with a phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) gene mutation rate of approximately 16% and gene amplification of more than 30% in tumors ^[6][7][6,7]. PI3Ks are activated by receptor tyrosine kinases (RTKs), such as epidermal growth factor receptor (EGFR), and consist of different classes of enzymes vital for differentiation, proliferation and cell survival [8]. Mammalian target of rapamycin (mTOR) complexes (mTORC1 and mTORC2) and protein kinase B (also known as AKT) are also involved in this pathway that can activate transcription and other signaling molecules of the PI3K pathway [9]. Monoclonal antibodies (mAbs) that inhibit EGFR have been used for both HPV(−) and HPV(+) subtypes of HNSCCs; however, they were found to have limited efficacy and elicited resistance [10].

Another highly mutated gene in HNSSCs is the tumor protein p53 (TP53) gene, with over 80% of HPV(−) HNSCCs harboring loss-of-function mutations in TP53; however, TP53 mutations occur much less frequently in HPV(+) HNSCCs (~3%) [4]. TP53 is a tumor-suppressor gene encoding a transcription factor that maintains DNA repair, cell cycle, senescence and apoptosis [11]. These attributes make p53 an important cell sensor for oncogene activation and DNA damage. It has been found that the degradation of p53 is associated with HPV E6 oncoproteins [3]. Although there have been several therapies that target p53 in hopes to restore p53 function, they have yet to be proven effective in clinical trials [12]. By and large, TP53 mutations are associated with poor HNSCC prognosis and overall survival with increased rate of recurrence and resistance to therapies. It remains poorly understood whether HNSCCs harboring different genetic alterations exhibit differential immune tumor microenvironment (TME). For instance, it is unknown whether HNSCCs with the double mutations in TP53 and PIK3CA have a more immunosuppressive TME.

Prior studies have generated murine models that mimicked the alterations of PIK3CA or p53 in HNSCCs. Transgenic mice that overexpressed wild-type PIK3CA in head and neck epithelium were generated; however, PIK3CA overexpression alone was not sufficient to initiate HNSCC formation [6]. Nevertheless, these PIK3CA Tg mice were much more susceptible to 4-nitroquinoline 1 oxide (4NQO)-induced HNSCC carcinogenesis [6]. Conditional deletion of p53 in mouse epithelial cells with K14.CrePR1 led to SCC development in about half of mice after 20 months [13]. To establish a mouse model that more closely resembles the genetic alterations in HNSCCs and allows us to better investigate immune evasion mechanisms of HNSCCs, we generated a novel genetic model by deleting p53 and constitutively activating PIK3CA in mouse keratin 15-expressing (K15⁺) stem cells, which leads to the development of multilineage tumors including SCCs, termed Keratin-15-p53-PIK3CA (KPPA) tumors.

2. HNSCC Patients with Double Genetic Alterations in PIK3CA and TP53 Exhibited Worse Prognosis and More Immunosuppressive TME

The Cancer Genome Atlas (TCGA) RNA-seq data and clinical data of HNSCC cohorts were obtained from the cBioPortal (hhttps://cbioportal.org) (see dettp://cbails ioportal.orgn Methods). In analyzing the dataset of HNSCC patients (TCGA, PanCancer Atlas, n = 489 samples), we found that the patients with PIK3CA alterations, including amplification and gain, also have a higher chance of harboring TP53 mutations (Figure 1A). After merging two datasets (see details in Methods), we divided the patients into four different groups: PIK3CA^Amp/TP53^Mutated (n = 294), PIK3CA^Amp/TP53^WT (n = 85), PIK3CA^WT/TP53^Mutated (n = 56), and PIK3CA^WT/TP53^WT (n = 54). Kaplan Meier curves of five-year survival were shown for four different groups, and PIK3CA^Amp/TP53^Mutated group exhibited a hazard ratio of 1.61 (95% CI, 0.94–2.75) compared to PIK3CA^WT/TP53^WT group (Figure 1B). PIK3CA^Amp/TP53^Mutated group’s 10-year survival hazard ratio was 1.8 (95% CI, 1.06–3.07), and showed a significantly worse prognosis than PIK3CA^WT/TP53^WT group (Supplemental Figure S1).

Figure 1. Analysis of The Cancer Genome Atlas (TCGA) datasets of Head and Neck Squamous cell carcinoma (HNSCC) patients. (A) Association between PIK3CA gene alterations and TP53 gene mutation in HNSCC patients (TCGA, PanCancer Atlas, n = 489 samples). The Cancer Genome Atlas (TCGA) RNA-seq data and clinical data of HNSCC cohorts were obtained from the cBioPortal (hhttps://cbioportal.org) (see dettp://cbails ioportal.orgn Method). Patients with PIK3CA^Amp/gain (n = 359) had a higher chance to harbor TP53 mutations than patients with PIK3CA^WT (n = 130). (B) Kaplan-Meier overall 5-year survival curves of HNSCC patients in 4 groups (PIK3CA^Amp/TP53^Mutated, PIK3CA^Amp/TP53^WT, PIK3CA^WT/TP53^Mutated, and PIK3CA^WT/TP53^WT) as described in Methods. Only patients with available survival data were included for this analysis (n = 487). (C) A radar plot of the cell types that reached significance in the omnibus Kruskal-Wallis test when comparing among the 4 groups. The scale is per 1000 cells. (D) Box and whisker plots of CD8 T cells, resting macrophages (M0), and M1 macrophages. The expression of CD8 T cell signature genes: PIK3CA^Amp/TP53^Mutated group (3.77 ± 5.32) was significantly lower than groups of PIK3CA^WT/TP53^WT (8.97 ± 7.86) and PIK3CA^Amp/TP53^WT (8.22 ± 8.12). The expression of M0 signature genes: PIK3CA^Amp/TP53^Mutated group (21.07 ± 14.08) was significantly higher than groups of PIK3CA^WT/TP53^WT (15.25 ± 12.32) and PIK3CA^Amp/TP53^WT (13.93 ± 11.23). The expression of M1 signature genes: PIK3CA^Amp/TP53^Mutated group (5.35 ± 5.05) was significantly lower than groups of PIK3CA^WT/TP53^WT (8.17 ± 5.62) and PIK3CA^Amp/TP53^WT (6.92 ± 5.17).

We uploaded RNA-seq data of HNSCC patients onto CIBERSORT (see details in Methods), which estimated the relative proportions of 22 immune cell types (Supplemental Figure S2), with a more in-depth dissection shown in Supplemental Table S1. Both innate and adaptive immune cells varied in their expression levels depending on the genetic alterations in 4 groups (Figure 1C). In particular, we found that the expression of CD8 T cell signature genes was significantly lower in PIK3CA^Amp/TP53^Mutated group, compared with PIK3CA^WT/TP53^WT and PIK3CA^Amp/TP53^WT groups (Figure 1D). In addition, PIK3CA^Amp/TP53^Mutated group had significantly lower expression of activated NK cell-associated genes compared with PIK3CA^WT/TP53^WT and PIK3CA^WT/TP53^Mutated groups (Supplemental Table S1). PIK3CA^Amp/TP53^Mutated group expressed significantly higher level of resting macrophage (M0) signature genes but lower level of activated macrophage (M1) genes than PIK3CA^WT/TP53^WT and PIK3CA^Amp/TP53^WT groups (Figure 1D). We conclude that HNSCCs with the genotype of PIK3CA^Amp/TP53^Mutated appear to have a highly immunosuppressive TME.

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