Ecdysteroids: Comparison
Please note this is a comparison between Version 2 by Nora Tang and Version 1 by Rene LAFONT.

Ecdysteroid: member of a class of polyhydroxylated steroids found in invertebrate animals (zooecdysteroids; moulting hormones), plants (phytoecdysteroids) and fungi (mycoecdysteroids). Over 500 structural analogues are currently known. Biosynthetically, they derive from C27-, C28- or C29-sterols. The most frequently encountered analogue (in arthropods and plants) is 20-hydroxyecdysone (2β,3β,14α, 20R,22R,25-hexahydroxycholest-7-en-6-one). In arthropods, ecdysteroids occur universally and regulate development by inducing moulting and reproduction, where their action is mediated by high-affinity binding to an intracellular member of the class of nuclear receptor (NR) proteins (ecdysteroid receptor; EcR) dimerised with a second NR (USP/RxR). This receptor complex binds to specific DNA promoter sites and regulates gene expression. In plants, ecdysteroids are a class of secondary compounds, occurring in varying amounts in certain species, but not all in others. Phytoecdysteroids are believed to contribute to the reduction of invertebrate predation by acting as feeding deterrents or endocrine disruptors. Ecdysteroids also possess a wide range of positive pharmacological effects in mammals, where the mode of action involves moderate-affinity binding to plasma-membrane-bound receptors and not interaction with the classical NRs for vertebrate steroid hormones.

  • antifeedant
  • chemotaxonomy
  • crop protection
  • ecdysone
  • 20-hydroxyecdysone
  • moulting hormone
  • nutritional supplement
  • pharmaceutical
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References

  1. Koolman, J. (Ed.) Ecdysone: From Chemistry of Mode of Action; Thieme Verlag: Stuttgart, Germany, 1989; 482p.
  2. Dinan, L.; Savchenko, T.; Whiting, P. On the distribution of phytoecdysteroids in plants. Cell Mol. Life Sci. 2001, 58, 1121–1132.
  3. Dinan, L.; Harmatha, J.; Volodin, V.; Lafont, R. Phytoecdysteroids: Diversity, biosynthesis and distribution. In Ecdysone: Structures and Functions; Smagghe, G., Ed.; Springer Science & Business Media B.V.: Berlin, Germany, 2009; pp. 3–45.
  4. Lafont, R.; Harmatha, J.; Marion-Poll, F.; Dinan, L.; Wilson, I.D. The Ecdysone Handbook, 3rd ed.; Cybersales: Prague, Czech Republic, 2002; Available online: http://ecdybase.org/ (accessed on 1 April 2021).
  5. Butenandt, A.; Karlson, P. Über die Isolierung eines Metamorphose-Hormons der Insekten in kristallisierter Form. Z. Naturforsch. 1954, 9b, 389–391.
  6. Huber, R.; Hoppe, W. Zur Chemie des Ecdysons, VII: Die Kristall- und Molekülstrukturanalyse des Insektenverpuppungshormons ecdyson mit der automatisierten Faltmolekülmethode. Chem. Ber. 1965, 98, 2403–2424.
  7. Kannangara, J.R.; Mirth, C.K.; Warr, C.G. Regulation of ecdysone production in Drosophila by neuropeptides and peptide hormones. Open Biol. 2021, 11, 200373.
  8. Gilbert, L.I. Insect Endocrinology; Academic Press: London, UK, 2012; 577p.
  9. Belles, X. Insect Metamorphosis. From Natural History to Regulation of Developement and Evolution; Academic Press: London, UK, 2020; 289p.
  10. Denlinger, D.L.; Yocum, G.D.; Rinehart, J.P. Hormonal control of diapause. In Insect Endocrinology; Gilbert, L.I., Ed.; Academic Press: London, UK, 2012; pp. 430–463.
  11. Lafont, R.; Dauphin-Villemant, C.; Warren, J.T.; Rees, H. Ecdysteroid chemistry and biochemistry. In Insect Endocrinology; Gilbert, L.I., Ed.; Academic Press: London, UK, 2012; pp. 106–176.
  12. Yamanaka, N.; Okamoto, N. Molecular functions of ecdysteroids in insects. In Advances in Invertebrate (Neuro)endocrinology; Saleuddin, S., Lange, A.B., Orchard, I., Eds.; Apple Academic Press: Palm Bay, FL, USA, 2020; Volume 2, pp. 77–127.
  13. Pan, X.; Connacher, R.P.; O’Connor, M.B. Control of the insect metamorphic transition by ecdysteroid production and secretion. Curr. Opin. Insect Sci. 2021, 43, 11–20.
  14. Clever, U.; Karlson, P. Induktion von Puff-Verändungen in den Speichedrüsenchromosomen von Chironomus tentans durch Ecdyson. Exp. Cell. Res. 1960, 20, 623–626.
  15. Karlson, P. Chemistry and biochemistry of insect hormones. Angew. Chem. Internat. Edit. 1963, 2, 175–182.
  16. Ashburner, M.; Chihara, C.; Meltzer, P.; Richards, G. Temporal control of puffing activity in polytene chromosomes. Cold Spring Harb. Symp. Quant. Biol. 1974, 38, 662–665.
  17. Thummel, C.S. Ecdysone-regulated puff genes 2000. Insect Biochem. Molec. Biol. 2002, 32, 113–120.
  18. Nakanishi, K.; Koreeda, M.; Sasaki, S.; Chang, M.L.; Hsu, H.Y. Insect hormones. The structure of ponasterone A, an insect moulting hormone from the leaves of Podocarpus nakaii Hay. J. Chem. Soc. Chem. Commun. 1966, 915–917.
  19. Takemoto, T.; Ogawa, S.; Nishimoto, N. Isolation of the moulting hormones of insects from Achyranthes radix. Yakugaku Zasshi 1967, 87, 325–327.
  20. Galbraith, M.N.; Horn, D.H.S. An insect-moulting hormone from a plant. J. Chem. Soc. Chem. Commun. 1966, 905–906.
  21. Jizba, J.; Herout, V.; Sorm, F. Isolation of ecdysterone (crustecdysone) from Polypodium vulgare L. rhizomes. Tetrahedron Lett. 1967, 8, 1689–1691.
  22. Heinrich, G.; Hoffmeister, H. Ecdyson als Begleitsubstanz des Ecdysterons in Polypodium vulgare L. Experientia 1967, 23, 995.
  23. Kaplanis, J.N.; Thompson, M.J.; Robbins, W.E.; Bryce, M. Insect hormones: Alpha ecdysone and 20-hydroxyecdysone in bracken fern. Science 1967, 157, 1436–1438.
  24. Hocks, P.; Wiechert, R. 20-Hydroxyecdyson, isoliert aus Insekten. Tetrahedron Lett. 1966, 7, 2989–2993.
  25. Koreeda, M.; Nakanishi, K. 5β-Hydroxy-ecdysones and a revision of the structure of ponasterone C. Chem. Commun. 1970, 351–352.
  26. Blunt, J.W.; Lane, G.A.; Munro, M.H.G.; Russell, G.B. The absolute configuration at C-24 of the ecdysteroids dacrysterone, pterosterone and ponasterone C. Aust. J. Chem. 1979, 32, 779–782.
  27. Matsuoka, T.; Imai, S.; Sakai, M.; Kamada, M. Studies on phytoecdysones—A review of our works. Ann. Rep. Takeda Res. Lab 1969, 28, 221–271.
  28. Yen, K.-Y.; Yang, L.-L.; Okuyami, T.; Hikino, H.; Takemoto, T. Screening of Formosan ferns for phytoecdysones. I. Chem. Pharm. Bull. 1974, 22, 805–808.
  29. Volodin, V.; Chadin, I.; Whiting, P.; Dinan, L. Screening plants of European North-East Russia for ecdysteroids. Biochem. Syst. Ecol. 2002, 30, 525–578.
  30. Lafont, R.; Ho, R.; Raharivelomanana, P.; Dinan, L. Ecdysteroids in Ferns: Distribution, Diversity, Biosynthesis, and Functions. In Working with Ferns: Issues and Applications; Fernández, H., Kumar, A., Revilla, M.A., Eds.; Springer Science+Business Media: New York, NY, USA, 2011; pp. 305–319.
  31. Kovganko, N.V. Ecdysteroids and related compounds in fungi. Chem. Nat. Comp. 1999, 35, 597–611.
  32. Dinan, L.; Mamadalieva, N.; Lafont, R. The occurrence of ecdysteroids and ecdysteroid-related compounds in edible mushroom species. In Ecdybase.org; Cybersales: Prague, Czech Republic, 2019.
  33. Dinan, L. Distribution and levels of phytoecdysteroids within individual plants of species of Chenopodiaceae. Eur. J. Entomol. 1995, 92, 295–300.
  34. Chase, M.W.; Christenhusz, M.J.M.; Fay, M.F.; Byng, J.W.; Judd, W.S.; Mabberley, D.J.; Sennikov, A.N.; Soltis, P.S.; Stevens, P.F.; The Angiosperm Phylogeny Group. An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG IV. Bot. J. Linn. Soc. 2016, 181, 1–20.
  35. Dinan, L. Phytoecdysteroids: Biological aspects. Phytochemistry 2001, 57, 325–339.
  36. Tsukagoshi, Y.; Ohyama, K.; Seki, H.; Akashi, T.; Muranaka, T.; Suzuki, H.; Fujimoto, Y. Functional characterization of CYP71D443, a cytochrome P450 catalyzing C-22 hydroxylation in the 20-hydroxyecdysone biosynthesis of Ajuga hairy roots. Phytochemistry 2016, 127, 23–28.
  37. Dinan, L. Ecdysteroid structure-activity relationships. In Studies in Natural Product Chemistry 29; Atta-ur-Rahman, Ed.; Elsevier B.V.: Amsterdam, The Netherlands, 2003; pp. 3–71.
  38. Báthori, M.; Tóth, N.; Hunyadi, A.; Márki, A.; Zador, E. Phytoecdysteroids and anabolic-androgenic steroids—Structure and effects on Humans. Curr. Med. Chem. 2008, 15, 75–91.
  39. Harmatha, J.; Budesinsky, M.; Vokac, K. Photochemical transformation of 20-hydroxyecdysone: Production of monomeric and dimeric ecdysteroid analogues. Steroids 2002, 67, 127–135.
  40. Hunyadi, A.; Csábi, J.; Martins, A.; Molnár, J.; Balázs, A.; Tóth, G. Backstabbing P-gp: Side-chain cleaved ecdysteroid 2,3-dioxolanes hyper-sensitize MDR cancer cells to doxorubicin without efflux inhibition. Molecules 2017, 22, 199.
  41. Issaadi, H.M.; Béni, Z.; Tóth, T.; Dékány, M.; Hsieh, T.-J.; Balogh, G.T.; Hunyadi, A. Diversity-oriented synthesis through gamma radiolysis: Preparation of unusual ecdysteroid derivatives activating Akt and AMPK in skeletal muscle cells. Bioorg. Chem. 2021, 112, 104951.
  42. Dinan, L.; Balducci, C.; Guibout, L.; Lafont, R. Database of Human food plants and whether they have been assessed for the presence or absence of ecdysteroids. In Ecdybase.org; Cybersales: Prague, Czech Republic, 2019.
  43. Dinan, L.; Mamadalieva, N.; Lafont, R. Dietary phytoecdysteroids. In Handbook of Dietary Phytochemicals; Xiao, J., Sarker, S.D., Asakawa, Y., Eds.; Springer Nature Singapore Pte Ltd.: Singapore, 2020; pp. 1–54.
  44. Dinan, L.; Balducci, C.; Guibout, L.; Lafont, R. Small-scale analysis of phytoecdysteroids in seeds by HPLC-DAD-MS for the identification and quantification of specific analogues, dereplication and chemotaxonomy. Phytochem. Anal. 2020, 31, 643–661.
  45. Dinan, L.; Lafont, R. Compilation of the literature reports for the screening of vascular plants, algae, fungi and non-arthropod invertebrates for the presence of ecdysteroids. In Ecdybase.org; Cybersales: Prague, Czech Republic, 2019.
  46. Hendrix, S.D.; Jones, R.L. The activity of β-ecdysone in four gibberellin bioassays. Plant Physiol. 1972, 50, 199–200.
  47. Dreier, S.I.; Towers, G.H.N. Activity of ecdysterone in selected plant growth bioassays. J. Plant Physiol. 1988, 132, 509–512.
  48. Machackova, I.; Vagner, M.; Sláma, K. Comparison between the effects of 20-hydroxyecdysone and phytohormones on growth and development in plants. Eur. J. Entomol. 1995, 92, 309–316.
  49. Tarkowská, D.; Strnad, M. Plant ecdysteroids: Plant sterols with intriguing distributions, biological effects and relations to plant hormones. Planta 2016, 244, 545–555.
  50. Bakrim, A.; Lamhamdi, M.; Sayah, F.; Chibi, F. Effect of plant hormones and 20-hydroxyecdysone on tomato (Lycopersicum esculentum) seed germination and seedlings growth. Afr. J. Biotechnol. 2007, 6, 2792–2802.
  51. Turk, H. Progesterone promotes mitochondrial respiration at the biochemical and molecular level in germinating maize seed. Plants 2021, 10, 1326.
  52. Bajguz, A.; Koronka, A. Effect of ecdysone application on the growth and biochemical changes in Chlorella vulgaris cells. Plant Physiol. Biochem. 2001, 39, 707–715.
  53. Bajguz, A.; Dinan, L. Effects of ecdysteroids on Chlorella vulgaris. Physiol. Plant. 2004, 121, 349–357.
  54. Maršálek, B.; Šimek, M.; Smith, R.J. The effect of ecdysone on the cyanobacterium Nostoc 6720. Z. Naturforsch. 1992, 47c, 726–730.
  55. Uhlik, O.; Kamlar, M.; Kohout, L.; Jezek, R.; Harmatha, J.; Macek, T. Affinity chromatography reveals RuBisCO as an ecdysteroid-binding protein. Steroids 2008, 73, 1433–1440.
  56. Marion-Poll, F.; Dinan, L.; Lafont, R. The role of phytoecdysteroids in the battle against phytophagous insects. In Biopesticides of Plant Origin; Regnault-Roger, C., Philogène, B.J.R., Vincent, C., Eds.; Lavoisier: Paris, France, 2008; pp. 301–319.
  57. Marion-Poll, F. Chapter 4: Tasting toxicants as bitter: Phytoecdysteroids. In SEB Experimental Biological Series; Taste, I., Newland, P., Cobb, M., Marion-Poll, F., Eds.; Taylor and Francis: London, UK, 2009; Volume 63, pp. 127–138.
  58. Bergamasco, R.; Horn, D.H.S. Distribution and role of insect hormones in plants. In Endocrinology of Insects; A. R. Liss Inc.: New York, NY, USA, 1983; pp. 627–654.
  59. Kubo, I.; Hanke, F.J. Chemical methods for isolating and identifying phytochemicals biologically active in insects. In Insect Plant Interactions; Miller, J.R., Miller, T.A., Eds.; Springer: New York, NY, USA, 1986; pp. 225–249.
  60. Blackford, M.; Clarke, B.; Dinan, L. Tolerance of Spodoptera littoralis (Lepidoptera: Noctuidae) to ingested phytoecdysteroids. J. Insect Physiol. 1996, 42, 931–936.
  61. Blackford, M.J.P.; Clarke, B.S.; Dinan, L. Distribution and metabolism ofexogenous ecdysteroids in the Egyptian Cotton Leafworm Spodoptera littoralis (Lepidoptera: Noctuidae). Arch. Insect Biochem. Physiol. 1997, 34, 329–346.
  62. Blackford, M.; Dinan, L. The tomato moth Lacanobia oleracea (Lepidoptera: Noctuidae) detoxifies ingested 20-hydroxyecdysone, but is susceptible to the ecdysteroid agonists RH-5849 and RH-5992. Insect Biochem. Molec. Biol. 1997, 27, 167–177.
  63. Blackford, M.; Dinan, L. The effects of ingested ecdysteroid agonists (20-hydroxyecdysone, RH5849 and RH5992) and an ecdysteroid antagonist (cucurbitacin B) on larval development of two polyphagous lepidopterans (Acherontia atropos and Lacanobia oleracea). Entomol. Exp. Appl. 1997, 83, 263–276.
  64. Blackford, M.; Dinan, L. The effects of ingested 20-hydroxyecdysone on the larvae of Aglais urticae, Inachis io, Cynthia cardui (Lepidoptera: Nymphalidae) and Tyria jacobaeae (Lepidoptera: Actiidae). J. Insect Physiol. 1997, 43, 315–327.
  65. Schmelz, E.A.; Grebenok, R.J.; Galbraith, D.W.; Bowers, W.S. Damage-induced accumulation of phytoecdysteroids in spinach: A rapid root response involving the octadecanoic acid pathway. J. Chem. Ecol. 1998, 24, 339–360.
  66. Schmelz, E.A.; Grebenok, R.J.; Galbraith, D.W.; Bowers, W.S. Insect-induced synthesis of phytoecdysteroids in spinach, Spinacia oleracea. J. Chem. Ecol. 1999, 25, 1739–1757.
  67. Soriano, I.R.; Riley, I.T.; Potter, M.J.; Bowers, W.S. Phytoecdysteroids: A novel defense against plant-parasitic nematodes. J. Chem. Ecol. 2004, 30, 1885–1899.
  68. Prasuna, A.L.; Osmani, Z. Inhibitory effects of ecdysterone on the adult emergence of Achaea janata L. Sci. Cult. 1983, 49, 112–114.
  69. Arnault, C.; Sláma, K. Dietary effects of phytoecdysones in the leek-moth, Acrolepiosis assesctella Zell. (Lepidoptera: Acrolepiidae). J. Chem. Ecol. 1986, 12, 1979–1985.
  70. Tanaka, Y.; Naya, S.-I. Dietary effect of ecdysone and 20-hydroxyecdysone on larval development of two lepidopteran species. Appl. Entomol. Zool. 1995, 30, 285–294.
  71. Kubo, I.; Klocke, J.A.; Asano, S. Insect ecdysis inhibitors from the East African medicinal plant Ajuga remota (Labiatae). Agric. Biol. Chem. 1981, 45, 1925–1927.
  72. Tanaka, Y.; Takeda, S. Ecdysone and 20-hydroxyecdysone supplements to the diet affect larval development in the silkworm, Bombyx mori, differently. J. Insect Physiol. 1993, 39, 805–809.
  73. Zhang, M.; Kubo, I. Metabolic fate of ecdysteroids in larval Bombyx mori and Heliothis virescens. Insect Biochem. Mol. Biol. 1993, 7, 831–843.
  74. Tanaka, Y.; Asaoka, K.; Takeda, S. Different feeding and gustatory responses to ecdysone and 20-hydroxyecdysone by larvae of the silkworm, Bombyx mori. J. Chem. Ecol. 1994, 20, 125–133.
  75. Marion-Poll, F.; Descoins, C. Taste detection of phytoecdysteroids in larvae of Bombyx mori, Spodoptera littoralis and Ostrinia nubilalis. J. Insect Physiol. 2002, 48, 467–476.
  76. Schmelz, E.A.; Grebenok, R.J.; Ohnmeiss, T.E.; Bowers, W.S. Interactions between Spinacia oleracea and Bradysia impatiens: A role for phytoecdysteroids. Arch. Insect Biochem. Physiol. 2002, 51, 204–221.
  77. Kubo, I.; Komatsu, S.; Asaka, Y.; de Boer, G. Isolation and identification of apolar metabolites of ingested 20-hydroxyecdysone in frass of Heliothis virescens larvae. J. Chem. Ecol. 1987, 13, 785–794.
  78. Hoffmann, K.H.; Thiry, E.; Lafont, R. 14-Deoxyecdysteroids in an insect (Gryllus bimaculatus). Z. Naturforsch. 1990, 45c, 703–708.
  79. Thiry, E.; Hoffmann, K.H. Dynamics of ecdysone and 20-hydroxyecdysone metabolism after injection and ingestion in Gryllus bimaculatus. Zool. Jb. Physiol. 1992, 96, 17–38.
  80. Robinson, P.D.; Morgan, E.D.; Wilson, I.D.; Lafont, R. The metabolism of ingested and injected ecdysone by the final instar larvae of Heliothis armigera. Physiol. Entomol. 1987, 12, 321–330.
  81. Mondy, N.; Caissa, C.; Pitoizet, N.; Delbecque, J.-P.; Corio-Costet, M.-F. Effects of the ingestion of Serratula tinctoria extracts, a plant containing phytoecdysteroids, on the development of the vineyard pest Lobesia botrana (Lepidoptera: Tortricidae). Arch. Insect Biochem. Physiol. 1997, 35, 227–235.
  82. Feyereisen, R.; Lagueux, M.; Hoffmann, J.A. Dynamics of ecdysone metabolism after ingestion and injection in Locusta migratoria. Gen. Comp. Endocrinol. 1976, 29, 319–327.
  83. Modde, J.-F.; Lafont, R.; Hoffmann, J.A. Ecdysone metabolism in Locusta migratoria larvae and adults. Int. J. Invert. Reprod. Devel. 1984, 7, 161–183.
  84. Lafont, R. Phytoecdysteroids and moulting control. In New Strategies for Locust Control; Rembold, H., Ed.; ATSAF: Bonn, Germany, 1994; pp. 39–44.
  85. Weirich, G.F.; Thompson, M.J.; Svoboda, J.A. In vitro ecdysteroid conjugation by enzymes of Manduca sexta midgut cytosol. Arch. Insect Biochem. Physiol. 1986, 3, 109–126.
  86. Weirich, G.E.; Feldlaufer, M.F.; Svoboda, J.A. Ecdysone oxidase and 3-oxoecdysteroid reductases in Manduca sexta: Developmental changes and tissue distribution. Arch. Insect Biochem. Physiol. 1993, 23, 199–211.
  87. Rharrabe, K.; Alla, S.; Maria, A.; Sayah, F.; Lafont, R. Diversity of detoxification pathways of ingested ecdysteroids among phytophagous insects. Arch. Insect Biochem. Physiol. 2007, 65, 65–73.
  88. Rharrabe, K.; Maria, A.; Sayah, F.; Lafont, R. Metabolism of ingested 20-hydroxyecdysone in Plodia interpunctella larvae (Lepidoptera, Pyralidae). Moroccan J. Biol. 2008, 4/5, 55–62.
  89. Rharrabe, K.; Sayah, F.; Lafont, R. Dietary effects of four phytoecdysteroids on growth and development of the Indian meal moth, Plodia interpunctella. J. Insect Sci. 2010, 10, 13.
  90. Dhadialla, T.S.; Carlson, G.R.; Le, D.P. New insecticides with ecdysteroidal and juvenile hormone activity. Ann. Rev. Entomol. 1998, 43, 545–569.
  91. Chandrakala, M.; Maribashetty, V.; Jyoth, I.H. Application of phytoecdysteroids in sericulture. Curr. Sci. 1998, 74, 341–346.
  92. Trivedy, K.; Nirmal Kumar, S.; Dandin, S.B. Phytoecdysteroid and its use in sericulture. Sericologia 2006, 46, 57–78.
  93. Lafont, R.; Dinan, L. Innovative and future applications for ecdysteroids. In Ecdysone, Structures and Functions; Smagghe, G., Ed.; Georg Thieme-Verlag: Stuttgart, Germany, 2009; pp. 551–578.
  94. Sreejit, C.M.; Sasidharan, K.; Bose, C.; Thomas-Matthew, P.; Banerji, A. Effect of phytoecdysteroids isolated from Diploclisia glaucescens (BLUME) Diels and Coscinium fenestratum (Gaertn.) Colebr. and juvenile hormone analogue isolated from Cullen corylifolium (L.) Medik. on economic parameters of Bombyx mori L. under field condition. Int. J. Curr. Adv. Res. 2018, 7, 10746–10752.
  95. Kumar, D.; Maheshwari, G.; Pandey, J.P.; Kishore, R.; Rai, S.; Sahay, A. Phyto-ecdysteroids modulated synchronisation of cocoon-spinning in tasar silkworm, Antheraea mylitta D. Singapore J. Sci. Res. 2016, 6, 1–6.
  96. Kholodova, Y.D. Phytoecdysteroids: Biological effects, application in agriculture and complementary medicine. Ukr. Biokhim. Zh. 2001, 73, 21–29.
  97. Kanazawa, A.; Tanaka, N.; Kashiwada, K. Nutritional requirement of prawns-IV. The dietary effect of ecdysones. Bull. Jpn. Soc. Sci. Fish. 1972, 38, 1067–1071.
  98. Cho, G.; Itami, T. Plant extract as cholesterol substitute in shrimp. Aqua Feed. Formul. Beyond 2004, 1, 16–17. Available online: http://www.feedware.com/aqua/magazine/v1i2/additivesV1I22004.pdf (accessed on 1 April 2021).
  99. Waiho, K.; Ikhwanuddin, M.; Baylon, J.C.; Jalilah, M.; Rukminasari, N.; Fujaya, Y.; Fazhan, H. Moult induction methods in soft-shell crab production. Aquac. Res. 2021, 52, 4026–4042.
  100. Lehmann, M.; Vorbrodt, H.-M.; Adam, G.; Koolman, J. Antiecdysteroid activity of brassinosteroids. Experientia 1988, 44, 355–356.
  101. Voigt, B.; Whiting, P.; Dinan, D. The ecdysteroid agonist/antagonist and brassinosteroid-like activities of synthetic brassinosteroid/ecdysteroid hybrid molecules. Cell. Mol. Life Sci. 2001, 58, 1133–1140.
  102. Tarkowská, D.; Krampolová, E.; Strnad, M. Plant triterpenoid crosstalk: The interaction of brassinosteroids and phytoecdysteroids in Lepidium sativum. Plants 2020, 9, 1325.
  103. Thussagunpanit, J.; Jutamanee, K.; Homvisasevongsa, S.; Suksamrarn, A.; Yamagami, A.; Nakano, T.; Asami, T. Characterization of synthetic ecdysteroid analogues as functional mimics of brassinosteroids in plant growth. J. Steroid Biochem. Mol. Biol. 2017, 172, 1–8.
  104. Kovganko, N.V.; Ananich, S.K. Progress in the chemical synthesis of brassinosteroids. Chem. Nat. Compd. 2002, 38, 122–141.
  105. Kovganko, N.V.; Kashkan, Z.N.; Chernov, Y.G.; Ananich, S.K.; Sokolov, S.N.; Survillo, V.I. Synthesis of ecdysteroids and related compounds. Chem. Nat. Compd. 2003, 39, 411–437.
  106. Horn, D.H.S. The Ecdysones. In Naturally Occurring Insecticides; Jacobson, M., Crosby, D.G., Eds.; Marcell Dekker, Inc.: New York, NY, USA, 1971; pp. 333–459.
  107. Lafont, R.; Morgan, E.D.; Wilson, I.D. Chromatographic procedures for ecdysteroids. J. Chromatogr. A 1994, 658, 31–53.
  108. Báthori, M. Phytoecdysteroids effects on mammalians, isolation and analysis. Mini Rev. Med. Chem. 2002, 2, 285–293.
  109. Ho, R.; Girault, J.-P.; Cousteau, P.-Y.; Bianchini, J.-P.; Raharivelomana, P.; Lafon, T.R. Isolation of a new class of ecdysteroid conjugates (glucosyl-ferulates) using a combination of liquid chromatographic methods. J. Chromatogr. Sci. 2008, 46, 102–110.
  110. Sovová, H.; Opletal, L.; Sajfrtová, M.; Bártlová, M. Supercritical fluid extraction of cynaropicrin and 20-hydroxyecdysone from Leuzea carthamoides DC. J. Sep. Sci. 2008, 31, 1387–1392.
  111. Zheng, Y.; Liu, B.; Chen, M.; Chen, T. Supercritical fluid extraction of ecdysterone from the roots of Achyranthes bidentata BL. J. Sep. Sci. 2008, 31, 1393–1398.
  112. Debien, I.C.N.; Vardanega, R.; Santos, D.T.; Meireles, M.A.A. Optimization of pressurized liquid extraction of ecdysteroids from Brazilian Ginseng (Pfaffia glomerata) roots. In Proceedings of the III Iberoamerican Conference on Supercritical Fluids, Cartagena de Indias, Columbia, 1–5 April 2013; p. 9.
  113. Debien, C.N.; Meireles, M.A.A. Supercritical fluid extraction of beta-ecdysone from Brazilian ginseng (Pfaffia glomerata) roots. Food Public Health 2014, 4, 67–73.
  114. Bajkacz, S.; Rusin, K.; Wolny, A.; Adamek, J.; Erfurt, K.; Chorbok, A. Highly efficient extraction procedures based on natural deep eutectic solvents of ionic liquids for determination of 20-hydroxyecdysone in spinach. Molecules 2020, 25, 4736.
  115. Lafont, R.; Pennetier, J.-L.; Andrianjafintino, M.; Claret, J. Sample processing for high-performance liquid chromatography of ecdysteroids. J. Chromatogr. 1982, 236, 137–149.
  116. Wilson, I.D.; Morgan, E.D.; Murphy, S.J. Sample preparation for the chromatographic determination of ecdysteroids using solid-phase extraction methods. Anal. Chim. Acta 1990, 236, 145–155.
  117. Lafont, R.; Blais, C.; Harmatha, J.; Wilson, I.D. Ecdysteroids: Chromatography. Module in Chemistry, Molecular Sciences and Chemical Engineering; Elsevier: Amsterdam, The Netherlands, 2013; p. 15.
  118. Dinan, L.; Harmatha, J.; Lafont, R. TLC of sterols, steroids and related triterpenoids. In Thin Layer Chromatography in Phytochemistry; Waksmundska-Hajnos, M., Sherma, J., Kowalska, T., Eds.; CRC Press: Boca Raton, FL, USA, 2008; pp. 575–603.
  119. Báthori, M.; Szendrei, K.; Herke, I. Application of combined chromatographic techniques in the screening and purification of ecdysteroids. Chromatographia 1986, 21, 234–238.
  120. Báthori, M.; Pongrácz, Z.; Tóth, G.; Simon, A.; Kandra, L.; Kele, Z.; Ohmacht, R. Isolation of a new member of the ecdysteroid glycoside family: 2-deoxy-20-hydroxyecdysone 22-O-β-D-glucopyranoside. J. Chromatogr. Sci. 2002, 40, 409–415.
  121. Báthori, M.; Pongrácz, Z.; Ohmacht, R.; Mathé, I. Preparative scale purification of shidasterone, 2-deoxy-polypodine B and 9α,20-dihydroxyecdysone from Silene italica ssp. nemoralis. J. Chromatogr. Sci. 2004, 42, 275–279.
  122. Kubo, I.; Klocke, J.A.; Ganjian, I.; Ichikawa, N.; Matsumoto, T. Efficient isolation of ecdysteroids from Ajuga plants by high-performance liquid chromatography and droplet counter-current chromatography. J. Chromatogr. 1983, 257, 157–161.
  123. Fang, L.; Li, J.; Zhou, J.; Wang, X.; Guo, L. Isolation and purification of three ecdysteroids from the stems of Diploclisia glaucescens by high-speed countercurrent chromatography and their anti-inflammatory activities in vitro. Molecules 2017, 22, 1310.
  124. Issaadi, H.M.; Tsai, Y.-C.; Chang, F.-R.; Hunyadi, A. Centrifugal partition chromatography in the isolation of minor ecdysteroids from Cyanotis arachnoidea. J. Chromatogr. B 2017, 1054, 44–49.
  125. Lafont, R.; Martin-Sommé, G.; Chambet, J.-C. Separation of ecdysteroids by using high-pressure liquid chromatography on microparticulate supports. J. Chromatogr. 1979, 170, 185–194.
  126. Lafont, R.; Sommé-Martin, G.; Mauchamp, B.; Maume, B.F.; Delbecque, J.-P. Analysis of ecdysteroids by high-peformance liquid chromatography and coupled gas-liquid chromatography-mass spectrometry. In Progress in Ecdysone Research; Hofmann, J.A., Ed.; Elsevier/North Holland Biomedical Press: Amsterdam, The Netherlands, 1980; pp. 45–68.
  127. Lafont, R.; Kaouadji, N.; Morgan, E.D.; Wilson, I.D. Selectivity in the high-performance liquid chromatography of ecdysteroids. J. Chromatogr. A 1994, 658, 55–67.
  128. Dinan, L.; Harmatha, J.; Lafont, R. HPLC of steroids. In High Performance Liquid Chromatography in Phytochemical Analysis; Waksmundska-Hajnos, M., Sherma, J., Eds.; CRC Press: Boca Raton, FL, USA, 2011; pp. 679–708.
  129. Raynor, M.W.; Kithini, J.P.; Bartle, K.D.; Games, D.E.; Mylchreest, I.C.; Lafont, R.; Morgan, E.D.; Wilson, I.D. Packed column suercritical-fluid chromatography and linked supercritical-fluid chromatography-mass spectrometry for the analysis of phytoecdysteroids from Silene nutans and Silene otites. J. Chromatogr. 1989, 467, 292–298.
  130. Shim, J.-H.; Wilson, I.D.; Morgan, E.D. Boronic esters as derivatives for supercritical fluid chromatography of ecdysteroids. J. Chromatogr. A 1993, 639, 281–285.
  131. Isaac, R.E.; Rees, H.H. Isolation and identification of ecdysteroid phosphates and acetylecdysteroid phosphates from developing eggs of the locust, Schistocerca gregaria. Biochem. J. 1984, 221, 459–464.
  132. Evershed, R.P.; Prescott, M.C.; Kabbouh, M.; Rees, H.H. High-performance liquid chromatography/mass spectrometry with thermospray ionization of free ecdysteroids. Rapid Commun. Mass Spectrom. 1993, 7, 477–481.
  133. Girault, J.-P.; Lafont, R. The complete 1H-NMR assignment of ecdysone and 20-hydroxyecdysone. J. Insect Physiol. 1988, 7, 701–706.
  134. Hikino, H.; Okuyama, T.; Konno, C.; Takemoto, T. Carbon-13 nuclear magnetic resonance spectra of phytoecdysones. Chem. Pharm. Bull. 1975, 23, 125–132.
  135. Filho, J.G.S.; Duringer, J.; Mala, G.L.A.; Tavares, J.F.; Xavier, H.S.; da Silva, M.S.; da-Cunha, E.V.; Barbosa-Filho, J.M. Ecdysteroids from Vitex species: Distribution and compilation of their 13C-NMR spectral data. Chem. Biodivers. 2008, 5, 707–713.
  136. Girault, J.-P. Determination of ecdysteroids structure by 1D and 2D NMR. Russ. J. Plant Physiol. 1998, 45, 306–309.
  137. Wilson, I.D.; Morgan, E.D.; Lafont, R.; Wright, B. High-performance liquid chromatography coupled to nuclear magnetic resonance spectroscopy. Application to the ecdysteroids of Silene otites. J. Chromatogr. A 1998, 799, 333–336.
  138. Wilson, I.D.; Morgan, E.D.; Lafont, R.; Shockcor, J.P.; Lindon, J.C.; Nicholson, J.K.; Wright, B. High performance liquid chromatography coupled to nuclear magnetic resonance spectroscopy and mass spectrometry applied to plant products: Identification of ecdysteroids from Silene otites. Chromatographia 1999, 49, 374–378.
  139. Wilson, I.D. Multiple hyphenation of liquid chromatography with nuclear magnetic resonance spectroscopy, mass spectrometry and beyond. J. Chromatogr. A 2000, 892, 315–327.
  140. Louden, D.; Handley, A.; Taylor, S.; Lenz, E.; Miller, S.; Wilson, I.D.; Sage, A.; Lafont, R. Spectroscopic characterisation and identification of ecdysteroids using high-performance liquid chromatography combined with on-line UV-diode array, FT-infrared and 1H-nuclear magnetic resonance spectroscopy and time of flight mass spectrometry. J. Chromatogr. A 2001, 910, 237–246.
  141. Louden, D.; Handley, A.; Lafont, R.; Taylor, S.; Sinclair, I.; Lenz, E.; Orton, T.; Wilson, I.D. HPLC analysis of ecdysteroids in plant extracts using superheated deuterium oxide with multiple on-line spectroscopic analysis (UV, IR, 1H NMR, and MS). Anal. Chem. 2002, 74, 288–294.
  142. Claude, E.; Tower, M.; Lafont, R.; Wilson, I.D.; Plumb, R.S. High performance thin-layer chromatography of plant ecdysteroids coupled with desorption electrospray ionisation-ion mobility-time of flight high resolution mass spectrometry (HPTLC/DESI/IM/ToFMS). Chromatographia 2020, 83, 1029–1035.
  143. Jaroszewski, J.W. Hyphenated NMR methods in natural products research, part 1: Direct hyphenation. Planta Med. 2005, 71, 691–700.
  144. Borst, D.W.; O’Connor, J.D. Trace analysis of ecdysones by gas-liquid chromatography, radioimmunoassay and bioassay. Steroids 1974, 24, 637–656.
  145. Borst, D.W.; O’Connor, J.D. Arthropod molting hormone: Radioimmune assay. Science 1972, 178, 418–419.
  146. De Reggi, M.L.; Hirn, M.H.; Delaage, M.A. Radioimmunoassay of ecdysone an application to Drosophila larvae and pupae. Biochem. Biophys. Res. Comm. 1975, 66, 1307–1315.
  147. Porcheron, P.; Foucrier, J.; Gros, C.; Pradelles, P.; Cassier, P.; Dray, F. Radioimmunoassay of arthropod moulting hormone: β-ecdysone antibodies production and 125I-iodinated tracer preparation. FEBS Lett. 1976, 61, 159–162.
  148. Horn, D.H.; Wilkie, J.S.; Sage, B.A.; O’Connor, J.D. A high affinity antiserum specific for the ecdysone nucleus. J. Insect Physiol. 1976, 22, 901–905.
  149. Spindler, K.D.; Beckers, C.; Gröschel-Stewart, U.; Emmerich, H. A radioimmunoassay for arthropod moulting hormones, introducing a novel method of immunogen coupling. Hoppe Seylers Z Physiol Chem. 1978, 359, 1269–1272.
  150. Reum, L.; Koolman, J. Analysis of ecdysteroids by radioimmunoassay: Comparison of three different antisera. Insect Biochem. 1979, 9, 135–142.
  151. Soumoff, C.; Horn, D.H.; O’Connor, J.D. Production of a new antiserum to arthropod molting hormone and comparison with two other antisera. J. Steroid Biochem. 1981, 14, 429–435.
  152. Reum, L.; Klinger, W.; Koolman, J. A New Immunoassay for Ecdysteroids Based on Chemiluminescence; Kricka, L.J., Ed.; Academic Press: London, UK, 1984; pp. 249–252.
  153. Reum, L.; Koolman, J. Radioimmunoassay of ecdysteroids. In Ecdysone; Koolman, J., Ed.; Georg Thieme Verlag: Stuttgart, Germany, 1989; pp. 131–143.
  154. Warren, J.T.; Smith, W.; Gilbert, L.I. Simplification of the ecdysteroid radioimmunoassay by the use of protein A from Staphylococcus aureus. Experientia 1984, 40, 393–394.
  155. Porcheron, P.; Moriniere, M.; Grassi, J.; Pradelles, P. Development of an enzyme immunoassay for ecdysteroids using acetylcholinesterase as label. Insect Biochem. 1989, 19, 117–122.
  156. Royer, C.; Porcheron, P.; Pradelles, P.; Mauchamp, B. Development and use of an enzymatic tracer for an enzyme immunoassay of makisterone A. Insect Biochem. Mol. Biol. 1993, 23, 193–197.
  157. Von Glyscinski, U.; Delbecque, J.-P.; Böcking, D.; Sedlmeier, D.; Dirksen, H.; Lafont, R. Three new antisera with high sensitivity to ecdysone, 3-dehydroecdysone and other A-ring derivatives: Production and characterization. Eur. J. Entomol. 1995, 92, 75–79.
  158. Pascual, N.; Bellés, X.; Delbecque, J.-P.; Hua, Y.-J.; Koolman, J. Quantification of ecdysteroids by immunoassay: Comparison of enzyme immunoassay and radioimmunoassay. Z. Naturforsch. 1995, 50c, 862–867.
  159. McKinney, D.A.; Strand, M.R.; Brown, M.R. Evaluation of ecdysteroid antisera for a competitive enzyme immunoassay and extraction procedures for the measurement of mosquito ecdysteroids. Gen. Comp. Endocrinol. 2017, 253, 60–69.
  160. Cymborowski, B. Bioassays for ecdysteroids. In Ecdysone: From Chemistry to Mode of Action; Koolman, J., Ed.; Thieme Verlag: Stuttgart, Germany, 1989; pp. 144–149.
  161. Dinan, L. Ecdysteroid structure and hormonal activity. In Ecdysone: From Chemistry to Mode of Action; Koolman, J., Ed.; Thieme Verlag: Stuttgart, Germany, 1989; pp. 345–354.
  162. Cherbas, L.; Yonge, C.D.; Cherbas, P.; Williams, C.M. The morphological response of Kc-H cells to ecdysteroids: Hormonal specificity. Wilhelm Roux’ Arch. 1980, 189, 1–15.
  163. Clément, C.Y.; Bradbrook, D.A.; Lafont, R.; Dinan, L. Assessment of a microplate-based bioassay for the detection of ecdysteroid-like or antiecdysteroid activities. Insect Biochem. Molec. Biol. 1993, 23, 187–193.
  164. Dinan, L.; Hormann, R.E. Ecdysteroid agonists and antagonists. In Comprehensive Molecular Insect Science, Vol. 3, Endocrinology; Gilbert, L.I., Iatrou, K., Gill, S.S., Eds.; Elsevier: Amsterdam, The Netherlands, 2005; pp. 197–242.
  165. Wang, J.-L.; Ruan, D.-C.; Cheng, Z.-Y.; Yang, C.-R. The dynamic variations of 20-hydroxyecdysone in Cyanotis arachnoidea. Acta Bot. Yunnanica 1996, 18, 459–464.
  166. Bandara, B.M.R.; Jayasinghe, L.; Karunaratne, V.; Wanningama, G.P.; Bokel, M.; Kraus, W. Ecdysterone from stem of Diploclisia glaucescens. Phytochemistry 1989, 28, 1073–1075.
  167. Fujimoto, Y.; Ohyama, K.; Nomura, K.; Hyodo, R.; Takahashi, K.; Yamada, J.; Morisaki, M. Biosynthesis of sterols and ecdysteroids in Ajuga hairy roots. Lipids 2000, 35, 279–288.
  168. Duport, C.; Spagnoli, R.; Degryse, E.; Pompon, D. Self-sufficient biosynthesis of pregnenolone and progesterone in engineered yeast. Nat. Biotechnol. 1998, 16, 186–189.
  169. Szczerbara, F.M.; Chandelier, C.; Villeret, C.; Masurel, A.; Bourot, S.; Duport, C.; Blanchard, S.; Groisillier, A.; Testet, E.; Costaglioli, P.; et al. Total biosynthesis of hydrocortisone from a simple carbon source in yeast. Nat. Biotechnol. 2003, 21, 143–149.
  170. Dinan, L.; Dioh, W.; Veillet, S.; Lafont, R. 20-Hydroxyecdysone, from plant extracts to clinical use: Therapeutic potential for the treatment of neuromuscular, cardio-metabolic and respiratory diseases. Biomedicines 2021, 9, 492.
  171. Kametani, T.; Tsubuki, M. Strategies for the synthesis of ecdysteroids. In Ecdysone: From Chemistry to Mode of Action; Koolman, J., Ed.; Thieme Verlag: Stuttgart, Germany, 1989; pp. 74–96.
  172. Thiem, B.; Kikowska, M.; Malinski, M.P.; Kruszka, D.; Napierala, M.; Florek, E. Ecdysteroids: Production in plant in vitro cultures. Phytochem. Rev. 2017, 16, 603–622.
  173. John, R.; Shajitha, P.P.; Devassy, A.; Mathew, L. Effect of elicitation and precursor feeding on accumulation of 20-hydroxyecdysone in Achyranthes aspera Linn. cell suspension cultures. Physiol. Molec. Biol. Plants 2018, 24, 275–284.
  174. Malinski, M.P.; Kikowska, M.; Kruszka, D.; Napierala, M.; Florek, E.; Sliwinska, E.; Thiem, B. Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): A new potential source of phytoecdysteroids? Plant Cell Tissue Organ Cult. 2019, 139, 39–52.
  175. Corio-Costet, M.-F.; Chapuis, L.; Delbecque, J.-P.; Ustache, K. Genetic transformation of Serratula tinctoria (Dyer’s Savory) for ecdysteroid production. In Biotechnology in Agriculture and Forestry 45: Transgenic Medicinal Plants; Bajaj, Y.P.S., Ed.; Springer: Berlin/Heidelberg, Germany, 1999; pp. 284–297.
  176. Skala, E.; Grabkowska, R.; Sitarek, P.; Kuzma, L.; Blauz, A.; Wysokinska, H. Rhaponticum carthamoides regeneration through direct and indirect organogenesis, molecular profiles and secondary metabolite production. Plant Cell Tiss. Organ Cult. 2015, 123, 83–98.
  177. Chajra, H.; Salwinski, A.; Guillaumin, A.; Mignard, B.; Hannewald, P.; Duriot, L.; Warnault, P.; Guillet-Claude, C.; Fréchet, M.; Bourgaud, F. Plant Milking Technology—An Innovative and Sustainable Process to Produce Highly Active Extracts from Plant Roots. Molecules 2020, 25, 4162.
  178. Xu, S.; Li, Y. Yeast as a promising heterologous host for steroid bioproduction. J. Indust. Microbiol. Biotechnol. 2020, 47, 829–843.
  179. Chen, J.; Tang, J.; Yongyan, X.; Zhubo, D.; Bi, C.; Xi, C.; Fan, F.; Zhang, X. Production of 14α-hydroxysteroids by a recombinant Saccharomyces cerevisiae biocatalyst expressing of a fungal steroid 14α-hydroxylation system. Appl. Microbiol. Biotechnol. 2019, 103, 8363–8374.
  180. Findeisen, E. Ecdysteroide in Menschlicher Nahrung. Ph.D. Thesis, University of Marburg, Marburg, Germany, 2004.
  181. Grebenok, R.J.; Ripa, P.V.; Adler, J.H. Occurrence and levels of ecdysteroids in spinach. Lipids 1991, 26, 666–668.
  182. Kumpun, S.; Maria, A.; Crouzet, S.; Evrard-Todeschi, N.; Girault, J.-P.; Lafont, R. Ecdysteroids from Chenopodium quinoa Willd., an ancient Andean crop of high nutritional value. Food Chem. 2011, 125, 1226–1234.
  183. Sautour, M.; Canon, F.; Miyamoto, T.; Dongmo, A.; Lacaille-Dubois, M.A. A new ecdysteroid and other constituents from two Dioscorea species. Biochem. Syst. Ecol. 2008, 36, 559–563.
  184. Lafont, R.; Dinan, L. Practical uses for ecdysteroids in mammals including humans: An update. J. Insect Sci. 2003, 3, 7.
  185. Ambrosio, G.; Wirth, D.; Joseph, J.F.; Mazzarino, M.; de la Torre, X.; Botrè, F.; Parr, M.K. How reliable is dietary supplement labelling?—Experiences from the analysis of ecdysterone supplements. J. Pharm. Biomed. Anal. 2020, 177, 112877.
  186. Hunyadi, A.; Herke, I.; Lenglyel, K.; Báthori, M.; Kele, Z.; Simon, A.; Tóth, G.; Szendrei, K. Ecdysteroid-containing food supplements from Cyanotis arachnoidea on the European market: Evidence for spinach product counterfeiting. Sci. Rep. 2016, 6, 37322.
  187. Sláma, K.; Lafont, R. Insect hormones—Ecdysteroids: Their presence and action in vertebrates. Eur. J. Entomol. 1995, 92, 355–377.
  188. Matsuda, T.; Kawaba, T.; Yamamoto, Y. Pharmacological studies of insect metamorphosing steroids from Achyranthis radix. Nippon. Yakurigaku Zasshi (Folia Pharmacol. Jpn.) 1970, 66, 551–563.
  189. Ogawa, S.; Nishimoto, N.; Matsuda, H. Pharmacology of ecdysones in Vertebrates. In Invertebrate Endocrinology and Hormonal Heterophylly; Burdette, W.J., Ed.; Springer: Berlin, Germany, 1974; pp. 341–344.
  190. Uchiyama, M.; Yoshida, T. Effect of ecdysterone on carbohydrate and lipid metabolism. In Invertebrate Endocrinology and Hormonal Heterophylly; Burdette, W.J., Ed.; Springer: Berlin, Germany, 1974; pp. 401–416.
  191. Uchiyama, M.; Otaka, T. Phytoecdysones and protein metabolism in mammalia. In Invertebrate Endocrinology and Hormonal Heterophylly; Burdette, W.J., Ed.; Springer: Berlin, Germany, 1974; pp. 375–400.
  192. Lafont, R.; Serova, M.; Didry-Barca, B.; Raynal, S.; Guibout, L.; Dinan, L.; Veillet, S.; Latil, M.; Dioh, W.; Dilda, P. 20-Hydroxyecdysone activates the protective arm of the RAAS via Mas receptor. BioRiv 2020.
  193. Parr, M.K.; Zhao, P.; Haupt, O.; Tchoukouegno Ngueu, S.; Hengevoss, J.; Fritzemeier, K.-H.; Piechotta, M.; Schlörer, N.; Muhn, P.; Zheng, W.-Y.; et al. Estrogen receptor beta is involved in skeletal muscle hypertrophy induced by the phytoecdysteroid ecdysterone. Mol. Nutr. Food Res. 2014, 58, 1861–1872.
  194. Gorelick-Feldman, J.; Cohick, W.; Raskin, I. Ecdysteroids elicit a rapid Ca2+ flux leading to Akt activation and increased protein synthesis in skeletal muscles. Steroids 2010, 75, 632–637.
  195. Detmar, M.; Dumas, M.; Bonté, F.; Meybeck, A.; Orfanos, C.E. Effects of ecdysterone on the differentiation of normal human keratinocytes in vitro. Eur. J. Dermatol. 1994, 4, 558–562.
  196. Syrov, V.N.; Khushbaktova, Z.A. Wound-healing effect of ecdysteroids. Dokl Akad Nauk. Resp Uzb. 1996, 12, 47–50.
  197. Li, Y.L.; Wu, X.; Zhang, C.; Zhenggao, Y.; Wang, W. Effect of ecdysterone paste on wound healing in rabbits. J. Xinxiang Med Coll. 2008, 25, 116–118.
  198. Zhou, Y.; Wu, X.; Wu, C.; Zhang, Y.; Zhang, Z.; Wang, W. The healing promoting effect of ecdysterone on experimental skin wound in rabbit. J. Pract. Med. 2010, 14, 2494–2496.
  199. Ho, R.; Teai, T.; Meybeck, A.; Raharivelomanana, P. UV-protective effects of phytoecdysteroids from Microsorum grossum extracts on human dermal fibroblasts. Nat. Prod. Commun. 2015, 10, 33–36.
  200. Napierała, M.; Nawrot, J.; Gornowicz-Porowska, J.; Florek, E.; Moroch, A.; Admaski, Z.; Kroma, A.; Miechowicz, I.; Nowak, G. Separation and HPLC characterization of active natural steroids in a standardized extract from the Serratula coronata herb with antiseborrheic dermatitis activity. Int. J. Environ. Res. Public Health 2020, 17, 6453.
  201. Smetanin, B.Y. The Influence of Preparations of Plant Origin on Physical Work Capacity; The Russian Ministry of Public Health: Moscow, Russian, 1986.
  202. Simakin, S.Y.; Panyushkin, V.V.; Portugalov, S.N.; Kostina, L.V.; Martisorov, E.G. Combined application of preparation Ecdysten and product Bodrost during training in cyclic sports. Sports Sci. Bull. 1988, 2, 29–31.
  203. Gadzhieva, R.M.; Portugalov, S.N.; Paniushkin, V.V.; Kondrat’eva, I.I. A comparative study of the anabolic action of Ecdysten, Leveton and Prime Plus, preparations of plant origin. Eksp Klin Farmakol. 1995, 58, 46–48.
  204. Azizov, A.P.; Seifulla, R.D.; Ankudinova, I.A.; Kondrat’eva, I.I.; Borisova, I.G. Effect of the antioxidants elton and leveton on the physical work capacity of athletes. Eksp Klin Farmakol. 1998, 61, 60–62.
  205. Semenov, V. Trial of the Leuzea-containing preparation «Rus-Olympic» under extreme conditions. In Leuzea and Your Health; Antoshechkin, A., Ed.; Ceptima Publishing Inc.: Clearwater, FL, USA, 2000.
  206. Emirova, L.R.; Rozhkova, E.A.; Paniushkin, V.V.; Mirzolan, R.S.; Seifulla, N.R. The effects of cytamines and their combinations with Ecdysten, Apilak, Vitamax and Essentiale on the work capacity of athletes. Eksp Klin Farmakol. 2004, 67, 66–68.
  207. Wilborn, C.D.; Taylor, L.W.; Campbell, B.I.; Kerksick, C.; Rasmussen, C.J.; Greenwood, M.; Kreider, R.B. Effects of methoxyisoflavone, ecdysterone, and sulfo-polysaccharide supplementation on training adaptations in resistance-trained males. J. Int. Soc. Sports Nutr. 2006, 3, 19–27.
  208. Safarova, D.D.; Tursunov, N.B. Aspects of sports medicine: The effect of Ekdisten. Sci. Sports Curr. Trends 2016, 12, 52–57.
  209. Isenmann, E.; Ambrosio, G.; Joseph, J.F.; Mazzarino, M.; de la Torre, X.; Zimmer, P.; Kazlauskas, R.; Goebel, C.; Botrè, F.; Diel, P.; et al. Ecdysteroids as non-conventional anabolic agent: Performance enhancement by ecdysterone supplementation in humans. Arch. Toxicol. 2019, 93, 1807–1816.
  210. WADA. Summary of Major Modifications and Explanatory Notes. 2020 Prohibited List. Available online: https://www.wada-ama.org/sites/default/files/wada_2020_english_summary_of_modifications_.pdf (accessed on 1 April 2021).
  211. Dioh, W.; Chabane, M.; Tourette, C.; Azbekyan, A.; Morelot-Panzini, C.; Hajjar, L.A.; Lins, M.; Nair, G.B.; Whitehouse, T.; Mariani, J.; et al. Testing the efficacy and safety of BIO101, for the prevention of respiratory deterioration, in patients with COVID-19 pneumonia (COVA study): A structured summary of a study protocol for a randomised controlled trial. Trials 2021, 22, 42.
  212. Sláma, K. Ecdysteroids: Insect hormones, plant defensive factors or human medicine? Phytoparasitica 1993, 20, 3–8.
  213. Esposito, D.; Rathisanabapathy, T.; Poulev, A.; Komarnytsky, S.; Raskin, I. Akt-dependent anabolic activity of natural and synthetic brassinosteroids in rat skeletal muscle cells. J. Med. Chem. 2011, 54, 4057–4066.
  214. Esposito, D.; Kizelsztein, P.; Komarnytski, S.; Raskin, I. Hypoglycemic effects of brassinosteroid in diet-induced obese mice. Am. J. Physiol. Endocrinol. Metab. 2012, 303, E652–E658.
  215. Esposito, D.; Rathisanabapathy, T.; Schmidt, B.; Shakarjian, M.P.; Komarnytsky, S.; Raskin, I. Acceleration of cutaneous wound healing by brassinosteroids. Wound Repair Regen. 2013, 21, 688–696.
  216. Abu Bakar, M.H.; Azmi, M.N.; Shariff, K.A.; Tan, J.S. Withaferin A protects against high-fat diet-induced obesity via attenuation of oxidative stress, inflammation, and insulin resistance. Appl. Biochem. 2019, 188, 241–259.
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