TMDC Nanozymes: Application Perspective: Comparison
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Applications of TMDC NZs in different fields—starting from biosensing to different treatment fields like antibacterial, anti-inflammation activity and cancer therapy—are discussed in more details. 

  • nanozymes
  • transition metal dichalcogenides
  • biosensing
  • anticancer
  • antimicrobial
  • cytoprotection

1. Biosensing Applications

A biosensor is an analytical system that can detect a specific biological analyte and translate presence and/or concentration information into analytical data, such as electrical, optical, and thermal signals, using a simple, low-cost, and time-effective operation [1][2][3]. With the advent of nanotechnology, NZ biosensors, including TMDC-based, have witnessed enormous applicability in biomedical domain, particularly diagnostics, due to their intrinsic enzymatic capabilities [1]. To date, TMDC NZs have been used to detect a variety of biochemical analytes, including tiny biomolecules (such as glucose, cholesterol, glutathione (GSH), and cysteine) as well as macromolecules (e.g., proteins).
TMDC NZ-based biosensing strategies primarily take advantage of their POD-like activity, in which they can oxidize chromogenic substrates (such as TMB, ABTS, and OPD) in the presence of H2O2 to produce colored products that can be measured colorimetrically [4][5][6]. This NZ-based H2O2 biosensing is frequently coupled with analyte-specific oxidases such as glucose oxidase (GOx), cholesterol oxidase (ChOx), xanthine oxidase (XOx), and uricase to detect glucose, cholesterol, xanthine, and uric acid, respectively, in biological samples. First, a specific oxidase enzyme metabolizes the bioanalyte in the presence of oxygen to produce a specific acidic product and H2O2 as a byproduct. This H2O2 is further sensed colorimetrically by NZs as mentioned above. Notably, within the linear detection range, the intensity of color correlates directly with the amount of bioanalyte present in the samples. The GOx/WS2 biosensor system, for example, was used to detect glucose with a linear range of 5–300 μM and a detection limit of 2.9 μM [7]. Similarly, cholesterol was successfully detected at concentrations as low as 15 μM using a ChOx/Au nanoparticle-laden MoS2 nanoribbon system [8], whereas uricase/MoS2 nanoflakes sensor could detect uric acid within a range of 0.5–100 μM in human serum samples [9].
On the contrary, the detection regimes for cysteine and glutathione (GSH) differ substantially. The ability of these materials to prevent oxidation of colorimetric substrates or revert the oxidized colored product (produced via POD-/OD-like activity of NZs) to its pristine unoxidized form is the basis for their sensing [10]. The color intensity of the reaction mix is inversely proportional to the amount of cysteine or GSH present. Previously, WS2 nanomaterial with POD-like activity was used to estimate GSH levels as low as 0.061 nM and a linear detection range of 0.1–10 nM. GSH levels in human serum samples could be measured easily and without interference from other substances [10]. Similarly, cysteine was quantified using Hg2+ stimulated OD-like activity of MoS2 QDs-Ag NPs in the 1–100 μM range [11].
TMDC NZs can also be used to detect biomacromolecules, such as proteins, in a simple and label-free manner. To date, protein biosensing has been approached in a variety of ways. For instance, lipase was found to prevent POD-like activity of MoS2, allowing its detection at concentrations as low as 5 nM [12]. Other TMDC NZs-based protein detection strategies utilize nucleic acid aptamer probes due to their target (proteins or other biomolecules) selectivity, chemical stability, and ability to be synthesized in vitro [13]. ssDNA aptamer probe/MoS2 nanosheet system was used to detect carcinoembryonic antigen (CEA). In comparison to bare MoS2 nanosheets, the POD-like activity of aptamer/MoS2 was ~4.3 times higher, enabling greater oxidation of TMB substrate and consequently higher color intensity. However, when the target analyte, CEA, is present, the attached aptamer probe releases from the MoS2 nanosheet’s surface and binds with the protein, showing a reduced TMB oxidation. This drop in color intensity can be measured and is inversely proportional to the CEA concentration. Using this method, CEA could be detected in a linear range of 50–1000 ng/mL with the detection limit of 50 ng/mL [14]. Aptamer-anchored MoS2/PtCu nanocomposites with strong OD-like activity were used to detect mucin 1 positive cells with high sensitivity and selectivity. Cells such as MCF-7 and A549, which have mucin 1 overexpression, could be detected even in populations as small as 300 cells. The use of NZs with OD-like activity, as in this case, is often advantageous because it surpasses the use of cytotoxic H2O2, thus improving the biocompatibility and allowing the biosensor to be used in conjunction with living cells [15]. Besides, protein-specific antibodies [16] or antibody/aptamer probes [17] were also physically/chemically conjugated onto TMDC NZs to detect Salmonella typhimurium-specific surface proteins and human epididymis-specific protein 4 (HE4) proteins, respectively.
Table 1 summarizes some of the recent TMDC-based NZs that have been used for molecular and macromolecular biosensing so far.
Table 1. TMDC NZs for biosensing applications.
 TMDC NZs for biosensing applications.
Analyte DetectedNanozyme SystemActivityAssisting EnzymeDetection TypeSubstrate EmployedLinear RangeDetection LimitStabilityBiological SamplesRef.
H2O2MoS2POD-like
. Besides, F-MoS2, when coupled with hyaluronic acid (HA), could reduce the excess of ROS and prevent the depolymerization of HA in artificial synovial fluid [3664]. TMDC NZ with CAT-/SOD-like activity was used to mitigate the pathology of Alzheimer’s disease by targeting neuronal mitochondria with (3-carboxypropyl)triphenyl-phosphonium bromide-conjugated 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(PEG)-2000]-functionalized MoS2 QDs. When tested in vitro in murine-derived microglia BV2 cells, this nano-formulation dramatically decreased oxidative stress, downregulated pro-inflammatory cytokines, and elevated anti-inflammatory cytokines. Furthermore, in vitro (in BV2 cells) and in vivo (in an Alzheimer’s disease mouse model) tests revealed that these nanomaterials were able to reduce amyloid-beta (Aβ) aggregation-mediated neurotoxicity and eliminate Aβ aggregates. These were attributed to switching microglial polarization from pro-inflammatory M1 to anti-inflammatory M2, presenting a novel pathway to mitigate Alzheimer’s disease pathology [3728].
Table 12. TMDC NZs for therapeutic applications.
Applications TMDCs Material Activity Mimics Targeting Molecule (if Any) Therapeutic Mechanism Therapeutic Mediators Light Characteristics (if Involved) Activity Assessed Against In Vivo Evaluation Ref.
Microbial Cells Mammalian Cells
 ColorimetricTMB0.125–1.75 μM0.08 μM Lake water[18]
Disinfection and wound healing MoS2/rGO POD-like, OD-like, CAT-like   ROS-mediated H2O2 Xenon lamp (100 mW/cm2) Chloramphenicol-resistant E. coli and S. aureus   S. aureus-infected full-skin defect mice models [2050]
H2O2N-Doped MoS2POD-like ColorimetricTMB  6 months 
Fe3O4@MoS2-Ag POD-like   Ag+ ion-mediated toxicity, ROS-mediated, PTT H2O2, Ag[19]
+ ions NIR (808 nm, 1 W/cm2) E. coli     [3843] H2O2Au NRs-anchored MoS2/CPOD-like ColorimetricTMB10–200 μM
citraconic anhydride modified PEI-MoS2 POD-like1.82 μM Cancer cells   Disruption of surface charge, ROS-mediated H2O2, 2-nitrobenzaldehyde UV light (365 nm) E. coli and S. aureus[  20]
E. coli and S. aureus-infected full-skin defect mice models [2454] H2O2MoS2/PpyPOD-like ColorimetricTMB50–2000 μM45 μM  
WS2 QDs-Van@lipo POD-like, OD-like   ROS-mediated, PTT, Chemotherapy H2O2, vancomycine[21 NIR (808 nm, 1 W/cm2)]
E. coli and Mu50 (vancomycin-intermediate S. aureus strain)   Mice models with Mu50-infected abscess [2555] GlucoseMoS2POD-likeGOxColorimetricTMB5–150 μM1.2 μM 
Cu NW-supported MoS2 NSHuman serum[22]
POD-like   ROS-mediated, PTT H2O2 NIR (808 nm, 1 W/cm2) E. coli and S. aureus   MRSA-infected full-skin defect mice models [3936] Glucose
N-doped MoS2, N-doped WSMoS2 QDsPOD-likeGOxFluorometric 10–1500 μM5.16 μM Fetal bovine serum2[23]
POD-like   ROS-mediated H2O2   Ampicillin resistant E. coli and B. subtilis   Ampicillin resistant E. coli-infected full-skin defect mice models [4065] GlucosePTCA-MoS2POD-likeGOxColorimetricTMB20–800 µM18.3 μM2 months (at 4 °C)Human serum[24]
Lysozyme exfoliated MoS2 NSs POD-like   ROS-mediated H2O2   Ampicillin-resistant E. coli and B. subtilis     [2353GlucoseMoS2-MIL-101(Fe)POD-likeGOxColorimetricTMB0.01−15 μM0.01 μM1 monthHuman serum[25]
]
PEG-MoS2 NFs POD-like   ROS-mediated, Photothermal therapy (PTT) H2O2 NIR (808 nm, 1 W/cm2) Ampicillin-resistant E. coli and B. subtilis   Ampicillin resistant E. coli-infected full-skin defect mice models [2656] GlucoseMoS2@MgFe2O4POD-likeGOxColorimetric
CMSF-MoSeTMB, ABTS5–200 μM2 μM1 monthHuman serum[26]
2 NSs POD-like   ROS-mediated H2O2   E. coli and B. subtilis   E. coli-infected full-skin defect mice models [2252] GlucoseCysteine- MoS2 NFPOD-likeGOx
Anticancer therapyColorimetricABTS50–1000 μM33.51 μM Human serum[27 Glucose responsive, TMZ-loaded chitosan-MoS2 POD-like   ROS-mediated, GSH depletion, hypoxia induced TPZ activation H2O2 and TPZ]
    A549 cells A549 tumor-bearing mice models [3159] GlucoseDextran-MoSe2POD-likeGOxColorimetricTMB40–400 µM28 µM10 daysHuman serum[
AuNBPs@MoS2 POD-like28]
  ROS-mediated, PTT H2O2 NIR laser (808 nm, 2.0 W/cm2GlucoseChitosan-MoSe2POD-likeGOxColorimetricTMB5–60 µM0.71 μM>1 monthHuman serum[29]
)   HeLa cells   [3361]
LNP-PEG-PEI coated, Dox loaded MoS2 NFs POD-like LNP nucleolar translocation signal peptide ROS-mediated, CT, PTT, PDT Dox NIR laser (808 nm, 3.0 W/cm2)   4T1 cells 4T1 tumor-bearing mice models [3260] GlucoseSDS–MoS2POD-likeGOx
MoSe2/CoSe2ColorimetricTMB5–500 μM@PEG0.57 μM Human serum[ POD-like, CAT-like30]
  ROS-mediated, GSH depletion, PTT H2O2 NIR laser (808 nm, 1.0 W/cm2)   HepG2 cells Tumor-bearing mice models [3058] GlucoseAuNPs@MoS2 QDPOD-likeGOxColorimetricTMB20–400 μM0.068 μM12 days
Cytoprotection MoS2 NSHuman serum, tear and saliva CAT-like, SOD-like, POD-like   Scavenging oxidative stress species     E. coli and S. aureus[31 A549 cells  ]
[3563] GlucosePVP-MoS2 NPs
Neurodegeneration TPP-MoS2POD-likeGOxColorimetricTMB1000–10,000 μM320 μM Fetal bovine serum[32]
 QDs CAT-like, SOD-like TPP (mitochondrial targeting) Scavenging oxidative stress species       BV-2 cells Amyloid precursor protein/presenilin 1 (APP/PS1) double transgenic mice [3728] GlucoseWS2POD-likeGOxColorimetricTMB5–300 μM2.9 μM Human serum[
Osteoarthritis Fullerene-like MoS2 CAT-like, SOD-like33]
  Scavenging oxidative stress species       HUVECs   [3664] GlucoseWS2 NS + Ag NCsPOD-likeGOxChemiluminescenceSodium bicarbonate0.03–20 μM0.0013 μM Human serum[34]
GlucoseHemin-WS2POD-likeGOxColorimetricTMB5–200 µM1.5 μM Human serum[35]
GlucoseWSe2POD-likeGOxColorimetricTMB10–60 μM10 μM  [36]
GlucoseVS2POD-likeGOxColorimetricTMB5–250 µM1.5 µM  [37]
CholesterolMoS2 NSPOD-likeChOxColorimetricTMB2–200 μM0.76 μM Human serum[38]
CholesterolMoS2 nanoribbons–AuNPsPOD-likeChOxColorimetricTMB40–1000 μM15 μM Human serum[39]
CholesterolOxidized GSH-modified MoS2 NSsPOD-likeChOxColorimetricTMB5.36–800 μM5.36 μM Mouse serum[40]
GSHWS2 NSsPOD-like ColorimetricTMB0.1–10 nM0.061 nm Human serum[41]
Uric acidMoS2 NFsPOD-likeUricaseColorimetricTMB0.5–100 μM0.3 μM Human serum[42]
XanthineMoSe2POD-likeXOxColorimetricTMB10–320 µM1.964 μM Human serum[43]
CysteineMoS2 QDs-Ag NPs (stimulated by Hg (II) ion)OD-like ColorimetricTMB1–100 μM0.82 μM1 monthHuman serum[44]
CEAAptamer/MoS2 NSsPOD-like ColorimetricTMB50–1000 ng/mL50 ng/mL Human serum[45]
LipaseMoS2 NPsPOD-like ColorimetricTMB5–200 nM4.8 nM  [46]
Mucin 1Aptamer-MoS2/PtCuOD-likeNAColorimetricTMBNA300 cells of MCF-7 MCF-7, A549, HEK293, and HepG2[47]

2. Therapeutics

2.1. Antibacterial Activity

The annual increase in the cases of bacterial infections is one of the most challenging aspects of the global public health and safety [1848]. In particular, an alarming rise in bacterial drug resistance—as a result of uncontrolled use of antibiotics—has heightened concerns in this context [1949]. Significant efforts are being made to overcome this challenge through the development of novel antibacterial agents with greater efficacy and specificity than conventional antibiotics. Recently, a new type of antibacterial therapy with broadband antimicrobial capability, known as nanozyme-mediated antibacterial therapy (NABT), has been introduced. It entails the use of NZs, including TMDC-based, with POD-/OD-like enzymatic activities to regenerate ROS, which then exerts antibacterial effects via oxidation of the bacterial membrane’s polysaccharides, proteins, and lipids [2050].
The antibacterial activity of TMDC NZs, along with their relatively biocompatible nature, may be advantageous during the wound healing process. Bacterial infections have already been shown to delay healing by increasing inflammatory responses at the wound site [2151]. As a result, using appropriate antibacterial agents could help to restore and balance the accurate healing microenvironment and avoid any delays. MoSe2 nanosheets/carboxyl-modified silk fibroin based wound dressing exerted considerable antibacterial effects on Escherichia coli and Bacillus subtilis due to their POD-like activity. The studies were conducted both in vitro and in vivo in E. coli-infected full-skin defect mice model in the presence of low amounts of H2O2 [2252]. Another study used lysozyme, an enzyme capable of hydrolyzing bacterial cell wall peptidoglycan, as an exfoliating agent to generate MoS2 nanosheets. These nanomaterials demonstrated enhanced antibacterial activity against ampicillin-resistant E. coli and B. subtilis, which was attributed synergistically to the antibacterial activity of lysozyme and the POD-like activity of MoS2 nanosheets [2353].
Another intriguing study was conducted by Niu and his colleagues, who used a combination of citraconic anhydride-modified polyethyleneimine (PEI)-MoS2 nanosheets and a photoacid generator molecule, 2-nitrobenzaldehyde (2-NBA). When 2-NBA was exposed to 365 nm light, the pH of the solution decreased, which activated the POD-like activity of the NZs to produce ROS and impart antibacterial effects. Furthermore, the irradiation time changed the charge of the nanomaterial from negative to positive, thanks to the photoreactive characteristics of citraconic anhydride, allowing Gram selectivity for the developed antimicrobial system [2454].
Multimodal therapy is usually considered to be more efficient and effective at imparting antibacterial effects. NZs were combined with photothermal and chemotherapy in a study by encapsulating WS2 quantum dots (WS2 QDs) and vancomycin in a thermal-sensitive liposome. The use of WS2 QDs benefited in two ways: (i) their POD-like activity allowed the generation of ROS and (ii) their photothermal property resulted in heat generation (via 808 nm NIR laser irradiation), causing liposomal rupturing at the targeted site, resulting in a reduction in drug doses required. This anti-biofilm agent demonstrated excellent anti-biofilm activity, eradicating both E. coli and Mu50 (vancomycin-intermediate Staphylococcus aureus strain) both in vitro and in vivo [2555]. Owing to POD-like activity and photothermal properties, PEG-functionalized MoS2 nanoflowers imparted an efficient antimicrobial effect and improved wound healing rate in ampicillin-resistant E. coli-infected full-skin defect mice models [2656]. Another study used mesoporous ruthenium nanoparticle that was loaded and capped with ascorbic acid prodrug and hyaluronic acid, respectively. Ciprofloxacin-coated MoS2 nanosheets were further bound to the outer surface of the nanocomposite. Post-administration, hyaluronidase enzyme (produced by bacteria) would reduce the hyaluronic acid capping degradation and release of ascorbic acid and MoS2 at the infected wound site. Ascorbic acid/MoS2-mediated reactive radical generation, and ruthenium nanoparticles-mediated photothermal therapy, could synergistically eliminate multidrug-resistant bacterial strains in vitro. Furthermore, this therapeutic agent demonstrated promising efficacy in S. aureus-infected mice models (tested for biofilm dispersion inhibition) and S. aureus and Pseudomonas aeruginosa infected mice models (tested for wound healing). On the other hand, Ciprofloxacin loading did not affect the antibacterial potency of these nanocomposites [2718].

2.2. Cancer Therapy

Cancer is one of the leading causes of death due to its late diagnosis and insufficient effects of currently available treatments (e.g., chemotherapy, radiation therapy, and surgical treatment) [2829]. NZs, including those based on TMDC, have recently gained prominence in cancer treatment. NZ-mediated cancer therapy, like antibacterial systems, uses POD-/OD-like activities to generate ROS and cause cancer cells to die [2957].
NZ-mediated cancer therapy is often limited by the lower availability of intra-tumoral H2O2. To address this challenge, recently, MoSe2/CoSe2@PEG nanosheets were synthesized. Using dissolved O2 and photoexcited electrons, this system was able to produce H2O2 via a sequential single-electron transfer mechanism. Furthermore, this NZ system showed potent dual POD- and CAT-like activities, which ensured efficient generation of ●OH and O2, respectively. ●OH caused mitochondrial damage, whereas O2 alleviated hypoxia and served as a source of H2O2. The anticancer effects were amplified by the nanomaterial’s excellent photothermal characteristics, as well as redox disruptions (through intracellular GSH reduction). Besides, biodegradability and urinal/fecal elimination (within two weeks post-administration) are other notable features of this therapeutic system [3058].
In another study, a glucose-responsive, H2O2 self-supplying nano-catalytic reactor was developed by self-assembly of GOx, tirapazamine (TPZ) and chitosan on the surface of MoS2 nanosheets. The catalytic mechanisms involved in the cascade are as follows: (i) catalysis of intra-tumoral glucose by GOx (in the presence of O2) to produce H2O2 and lower the pH; (ii) utilization of H2O2 by POD-like activity of MoS2 nanosheets to produce ROS—to damage the cancer cells. Meanwhile, depletion of O2 would activate TPZ, whereas MoS2 could utilize GSH to disturb cellular redox balance, further amplifying the anticancer effects. This therapeutic agent demonstrated potent anticancer effects on A549 cells in vitro and A549 tumor-bearing mice models in vivo. In contrast, even at concentrations as high as 100 g/mL, no cytotoxicity was observed in normal human umbilical vein endothelial cells (HUVEC). Furthermore, under in vivo conditions, these nanomaterials did not accumulate in normal organs, but instead degraded and were cleared out of the body, indicating minimal toxicity to normal tissues [3159].
Another significant challenge in the field of nanomedicine is the development of advanced theranostic platforms with both therapeutic and diagnostic capabilities. In this regard, 3D porous MoS2 nanoflowers were synthesized, then loaded with doxorubicin and coated with PEG-PEI (conjugated with LIM Kinase 2 protein (LMP) nucleolar translocation signal peptide). LMP peptide improved the nanomaterials’ nuclear targetability in cancer cells. Thus, these materials were able to specifically target the cancer cells and could exert potent anticancer effects both in vitro (4T1 cells) and in vivo (4T1 tumor bearing mice model) through pH-responsive/NIR-enhanced doxorubicin delivery into the tumor cells, NIR-induced photothermal effects along with ROS generation due to POD-like activity of MoS2 nanoflowers. Furthermore, the excellent photoacoustic properties of these materials allowed for real-time tracking post-intravenous in the 4T1 tumor bearing mice models [3260]. A smart hybrid NZ based on MoS2-coated bipyramidal gold nanostructure was developed for anticancer therapy and two-photon bioimaging. This hybrid nanomaterial produced considerable ROS due to the POD-like activity of MoS2, which was augmented further by irradiation with 808 nm NIR laser due to localized plasmonic effects. Such synergistic ROS generation exerted significant anticancer effects in HeLa cells, as confirmed by two-photon luminescence imaging [3361].

2.3. Anti-Inflammatory Effect

Apart from the applications listed above, TMDC NZs, particularly those with CAT/SOD-like activity, have also been used as antioxidant materials to provide cytoprotective effects and treat inflammatory diseases/conditions such as osteoarthritis and neurodegeneration [3462][3563][3664]. For example, MoS2 nanosheets with CAT/SOD-like activity were synthesized those were able to quench and reduce the levels of free radicals like nitric oxide (●NO), ●OH, and nitrogen-centered free radicals (●DPPH). Furthermore, treating H2O2-exposed A549 cells with these nanomaterials dramatically reduced oxidative stress [3563]. Fullerene-like MoS2 (F-MoS2) is another interesting TMDC-NZs with CAT-/SOD-like activities under physiological settings that appropriate it for using for the non-surgical treatment of osteoarthritis. F-MoS2 was able to catalyze ●O2– into H2O2 and then produce water and O2. Interestingly F-MoS2 was used to protect HUVEC cells from oxidative stress induced by H2O2

References

  1. Cai, S.; Yang, R. Two-Dimensional Nanomaterials with Enzyme-Like Properties for Biomedical Applications. Front. Chem. 2020, 8, 1109. Guo, X.; Wang, Y.; Wu, F.; Ni, Y.; Kokot, S. A colorimetric method of analysis for trace amounts of hydrogen peroxide with the use of the nano-properties of molybdenum disulfide. Analyst 2015, 140, 1119–1126.
  2. Mohankumar, P.; Ajayan, J.; Mohanraj, T.; Yasodharan, R. Recent developments in biosensors for healthcare and biomedical applications: A review. Measurement 2021, 167, 108293. Feng, L.; Zhang, L.; Zhang, S.; Chen, X.; Li, P.; Gao, Y.; Xie, S.; Zhang, A.; Wang, H. Plasma-Assisted Controllable Doping of Nitrogen into MoS2 Nanosheets as Efficient Nanozymes with Enhanced Peroxidase-Like Catalysis Activity. ACS Appl. Mater. Interfaces 2020, 12, 17547–17556.
  3. Makvandi, P.; Zarepour, A.; Zheng, X.; Agarwal, T.; Ghomi, M.; Sartorius, R.; Zare, E.N.; Zarrabi, A.; Wu, A.; Maiti, T.K.; et al. Non-spherical nanostructures in nanomedicine: From noble metal nanorods to transition metal dichalcogenide nanosheets. Appl. Mater. Today 2021, 24, 101107. Sun, H.; Gao, Y.; Hu, N.; Zhang, Y.; Guo, C.; Gao, G.; Ma, Z.; Ivan Ivanovich, K.; Qiu, Y. Electronic coupling between molybdenum disulfide and gold nanoparticles to enhance the peroxidase activity for the colorimetric immunoassays of hydrogen peroxide and cancer cells. J. Colloid Interface Sci. 2020, 578, 366–378.
  4. Feng, L.; Zhang, L.; Zhang, S.; Chen, X.; Li, P.; Gao, Y.; Xie, S.; Zhang, A.; Wang, H. Plasma-Assisted Controllable Doping of Nitrogen into MoS2 Nanosheets as Efficient Nanozymes with Enhanced Peroxidase-Like Catalysis Activity. ACS Appl. Mater. Interfaces 2020, 12, 17547–17556. Lei, J.; Lu, X.; Nie, G.; Jiang, Z.; Wang, C. One-Pot Synthesis of Algae-Like MoS2/PPy Nanocomposite: A Synergistic Catalyst with Superior Peroxidase-Like Catalytic Activity for H2O2 Detection. Part. Part Syst. Charact. 2015, 32, 886–892.
  5. Lei, J.; Lu, X.; Nie, G.; Jiang, Z.; Wang, C. One-Pot Synthesis of Algae-Like MoS2/PPy Nanocomposite: A Synergistic Catalyst with Superior Peroxidase-Like Catalytic Activity for H2O2 Detection. Part. Part Syst. Charact. 2015, 32, 886–892. Lin, T.; Zhong, L.; Guo, L.; Fu, F.; Chen, G. Seeing diabetes: Visual detection of glucose based on the intrinsic peroxidase-like activity of MoS2 nanosheets. Nanoscale 2014, 6, 11856–11862.
  6. Sun, H.; Gao, Y.; Hu, N.; Zhang, Y.; Guo, C.; Gao, G.; Ma, Z.; Ivan Ivanovich, K.; Qiu, Y. Electronic coupling between molybdenum disulfide and gold nanoparticles to enhance the peroxidase activity for the colorimetric immunoassays of hydrogen peroxide and cancer cells. J. Colloid Interface Sci. 2020, 578, 366–378. Wang, X.; Wu, Q.; Jiang, K.; Wang, C.; Zhang, C. One-step synthesis of water-soluble and highly fluorescent MoS2 quantum dots for detection of hydrogen peroxide and glucose. Sens. Actuators B Chem. 2017, 252, 183–190.
  7. Lin, T.; Zhong, L.; Song, Z.; Guo, L.; Wu, H.; Guo, Q.; Chen, Y.; Fu, F.; Chen, G. Visual detection of blood glucose based on peroxidase-like activity of WS2 nanosheets. Biosens. Bioelectron. 2014, 62, 302–307. Sreeramareddygari, M.; Somasundrum, M.; Surareungchai, W. In situ polymerization and covalent functionalisation of trithiocyanuric acid by MoS2 nanosheets resulting in a novel nanozyme with enhanced peroxidase activity. New J. Chem. 2020, 44, 5809–5818.
  8. Nirala, N.R.; Pandey, S.; Bansal, A.; Singh, V.K.; Mukherjee, B.; Saxena, P.S.; Srivastava, A. Different shades of cholesterol: Gold nanoparticles supported on MoS2 nanoribbons for enhanced colorimetric sensing of free cholesterol. Biosens. Bioelectron. 2015, 74, 207–213. Dong, W.; Chen, G.; Hu, X.; Zhang, X.; Shi, W.; Fu, Z. Molybdenum disulfides nanoflowers anchoring iron-based metal organic framework: A synergetic catalyst with superior peroxidase-mimicking activity for biosensing. Sens. Actuators B Chem. 2020, 305, 127530.
  9. Wang, X.; Yao, Q.; Tang, X.; Zhong, H.; Qiu, P.; Wang, X. A highly selective and sensitive colorimetric detection of uric acid in human serum based on MoS2-catalyzed oxidation TMB. Anal. Bioanal. Chem. 2019, 411, 943–952. Zhang, Y.; Zhou, Z.; Wen, F.; Tan, J.; Peng, T.; Luo, B.; Wang, H.; Yin, S. A flower-like MoS2-decorated MgFe2O4 nanocomposite: Mimicking peroxidase and colorimetric detection of H2O2 and glucose. Sens. Actuators B Chem. 2018, 275, 155–162.
  10. Li, L.; Wang, Q.; Chen, Z. Colorimetric detection of glutathione based on its inhibitory effect on the peroxidase-mimicking properties of WS2 nanosheets. Microchim. Acta 2019, 186, 257. Yu, J.; Ma, D.; Mei, L.; Gao, Q.; Yin, W.; Zhang, X.; Yan, L.; Gu, Z.; Ma, X.; Zhao, Y. Peroxidase-like activity of MoS2 nanoflakes with different modifications and their application for H2O2 and glucose detection. J. Mater. Chem. B 2018, 6, 487–498.
  11. Ojha, R.P.; Mishra, R.; Singh, P.; Nirala, N.R.; Prakash, R. A composite prepared from MoS2 quantum dots and silver nanoparticles and stimulated by mercury(II) is a robust oxidase mimetic for use in visual determination of cysteine. Microchim. Acta 2020, 187, 74. Jiang, G.; Lin, T.; Qin, Y.; Zhang, X.; Hou, L.; Sun, Y.; Huang, J.; Liu, S.; Zhao, S. Accelerating the peroxidase-like activity of MoSe2 nanosheets at physiological pH by dextran modification. Chem. Commun. 2020, 56, 10847–10850.
  12. Nandu, N.; Salih Hizir, M.; Roberston, N.M.; Ozturk, B.; Yigit, M.V. Masking the Peroxidase-Like Activity of the Molybdenum Disulfide Nanozyme Enables Label-Free Lipase Detection. ChemBioChem 2019, 20, 1861–1867. Liu, D.; Wang, D.; Jing, X.; Zhao, X.; Xi, D.; Dang, D.; Meng, L. Continuous phase regulation of MoSe2 from 2H to 1T for the optimization of peroxidase-like catalysis. J. Mater. Chem. B 2020, 8, 6451–6458.
  13. Bunka, D.H.J.; Stockley, P.G. Aptamers come of age—At last. Nat. Rev. Microbiol. 2006, 4, 588–596. Zhao, K.; Gu, W.; Zheng, S.; Zhang, C.; Xian, Y. SDS–MoS2 nanoparticles as highly-efficient peroxidase mimetics for colorimetric detection of H2O2 and glucose. Talanta 2015, 141, 47–52.
  14. Zhao, L.; Wang, J.; Su, D.; Zhang, Y.; Lu, H.; Yan, X.; Bai, J.; Gao, Y.; Lu, G. The DNA controllable peroxidase mimetic activity of MoS2 nanosheets for constructing a robust colorimetric biosensor. Nanoscale 2020, 12, 19420–19428. Vinita; Nirala, N.R.; Prakash, R. One step synthesis of 2-QDs composite as a robust peroxidase- mimetic for instant unaided eye detection of glucose in serum, saliva and tear. Sens. Actuators B Chem. 2018, 263, 109–119.
  15. Qi, C.; Cai, S.; Wang, X.; Li, J.; Lian, Z.; Sun, S.; Yang, R.; Wang, C. Enhanced oxidase/peroxidase-like activities of aptamer conjugated MoS2/PtCu nanocomposites and their biosensing application. RSC Adv. 2016, 6, 54949–54955. Yu, J.; Ma, X.; Yin, W.; Gu, Z. Synthesis of PVP-functionalized ultra-small MoS2 nanoparticles with intrinsic peroxidase-like activity for H2O2 and glucose detection. RSC Adv. 2016, 6, 81174–81183.
  16. Lu, L.; Ge, Y.; Wang, X.; Lu, Z.; Wang, T.; Zhang, H.; Du, S. Rapid and sensitive multimode detection of Salmonella typhimurium based on the photothermal effect and peroxidase-like activity of MoS2@Au nanocomposite. Sens. Actuators B Chem. 2021, 326, 128807. Lin, T.; Zhong, L.; Song, Z.; Guo, L.; Wu, H.; Guo, Q.; Chen, Y.; Fu, F.; Chen, G. Visual detection of blood glucose based on peroxidase-like activity of WS2 nanosheets. Biosens. Bioelectron. 2014, 62, 302–307.
  17. Zhang, S.; Chen, Y.; Huang, Y.; Dai, H.; Lin, Y. Design and application of proximity hybridization-based multiple stimuli-responsive immunosensing platform for ovarian cancer biomarker detection. Biosens. Bioelectron. 2020, 159, 112201. Khataee, A.; Haddad Irani-nezhad, M.; Hassanzadeh, J. Improved peroxidase mimetic activity of a mixture of WS2 nanosheets and silver nanoclusters for chemiluminescent quantification of H2O2 and glucose. Microchim. Acta 2018, 185, 190.
  18. Nikfarjam, N.; Ghomi, M.; Agarwal, T.; Hassanpour, M.; Sharifi, E.; Khorsandi, D.; Ali Khan, M.; Rossi, F.; Rossetti, A.; Nazarzadeh Zare, E.; et al. Antimicrobial Ionic Liquid-Based Materials for Biomedical Applications. Adv. Funct. Mater. 2021, 31, 2104148. Chen, Q.; Chen, J.; Gao, C.; Zhang, M.; Chen, J.; Qiu, H. Hemin-functionalized WS2 nanosheets as highly active peroxidase mimetics for label-free colorimetric detection of H2O2 and glucose. Analyst 2015, 140, 2857–2863.
  19. Tan, S.-A.; Agarwal, T.; Kar, S.; Borrelli, M.R.; Maiti, T.K.; Makvandi, P. A progressive review on paper-based bacterial colorimetric detection and antimicrobial susceptibility testing. In Food, Medical, and Environmental Applications of Polysaccharides; Pal, K., Banerjee, I., Sarkar, P., Bit, A., Kim, D., Anis, A., Maji, S., Eds.; Elsevier: Amsterdam, The Netherlands, 2021; pp. 687–718. ISBN 978-0-12-819239-9. Chen, T.M.; Wu, X.J.; Wang, J.X.; Yang, G.W. WSe2 few layers with enzyme mimic activity for high-sensitive and high-selective visual detection of glucose. Nanoscale 2017, 9, 11806–11813.
  20. Wang, L.; Gao, F.; Wang, A.; Chen, X.; Li, H.; Zhang, X.; Zheng, H.; Ji, R.; Li, B.; Yu, X.; et al. Defect-Rich Adhesive Molybdenum Disulfide/rGO Vertical Heterostructures with Enhanced Nanozyme Activity for Smart Bacterial Killing Application. Adv. Mater. 2020, 32, 2005423. Huang, L.; Zhu, W.; Zhang, W.; Chen, K.; Wang, J.; Wang, R.; Yang, Q.; Hu, N.; Suo, Y.; Wang, J. Layered vanadium(IV) disulfide nanosheets as a peroxidase-like nanozyme for colorimetric detection of glucose. Microchim. Acta 2018, 185, 7.
  21. Agarwal, T.; Tan, S.-A.; Onesto, V.; Law, J.X.; Agrawal, G.; Pal, S.; Lim, W.L.; Sharifi, E.; Moghaddam, F.D.; Maiti, T.K. Engineered herbal scaffolds for tissue repair and regeneration: Recent trends and technologies. Biomed. Eng. Adv. 2021, 2, 100015. Lin, T.; Zhong, L.; Chen, H.; Li, Z.; Song, Z.; Guo, L.; Fu, F. A sensitive colorimetric assay for cholesterol based on the peroxidase-like activity of MoS2 nanosheets. Microchim. Acta 2017, 184, 1233–1237.
  22. Huang, X.-W.; Wei, J.-J.; Liu, T.; Zhang, X.-L.; Bai, S.-M.; Yang, H.-H. Silk fibroin-assisted exfoliation and functionalization of transition metal dichalcogenide nanosheets for antibacterial wound dressings. Nanoscale 2017, 9, 17193–17198. Nirala, N.R.; Pandey, S.; Bansal, A.; Singh, V.K.; Mukherjee, B.; Saxena, P.S.; Srivastava, A. Different shades of cholesterol: Gold nanoparticles supported on MoS2 nanoribbons for enhanced colorimetric sensing of free cholesterol. Biosens. Bioelectron. 2015, 74, 207–213.
  23. Ma, D.; Xie, C.; Wang, T.; Mei, L.; Zhang, X.; Guo, Z.; Yin, W. Liquid-Phase Exfoliation and Functionalization of MoS2 Nanosheets for Effective Antibacterial Application. ChemBioChem 2020, 21, 2373–2380. Ma, D.; Yu, J.; Yin, W.; Zhang, X.; Mei, L.; Zu, Y.; An, L.; Gu, Z. Synthesis of Surface-Modification-Oriented Nanosized Molybdenum Disulfide with High Peroxidase-Like Catalytic Activity for H2O2 and Cholesterol Detection. Chem. A Eur. J. 2018, 24, 15868–15878.
  24. Niu, J.; Sun, Y.; Wang, F.; Zhao, C.; Ren, J.; Qu, X. Photomodulated Nanozyme Used for a Gram-Selective Antimicrobial. Chem. Mater. 2018, 30, 7027–7033. Li, L.; Wang, Q.; Chen, Z. Colorimetric detection of glutathione based on its inhibitory effect on the peroxidase-mimicking properties of WS2 nanosheets. Microchim. Acta 2019, 186, 257.
  25. Xu, M.; Hu, Y.; Xiao, Y.; Zhang, Y.; Sun, K.; Wu, T.; Lv, N.; Wang, W.; Ding, W.; Li, F.; et al. Near-Infrared-Controlled Nanoplatform Exploiting Photothermal Promotion of Peroxidase-like and OXD-like Activities for Potent Antibacterial and Anti-biofilm Therapies. ACS Appl. Mater. Interfaces 2020, 12, 50260–50274. Wang, X.; Yao, Q.; Tang, X.; Zhong, H.; Qiu, P.; Wang, X. A highly selective and sensitive colorimetric detection of uric acid in human serum based on MoS2-catalyzed oxidation TMB. Anal. Bioanal. Chem. 2019, 411, 943–952.
  26. Yin, W.; Yu, J.; Lv, F.; Yan, L.; Zheng, L.R.; Gu, Z.; Zhao, Y. Functionalized Nano-MoS2 with Peroxidase Catalytic and Near-Infrared Photothermal Activities for Safe and Synergetic Wound Antibacterial Applications. ACS Nano 2016, 10, 11000–11011. Wu, X.; Chen, T.; Wang, J.; Yang, G. Few-layered MoSe2 nanosheets as an efficient peroxidase nanozyme for highly sensitive colorimetric detection of H2O2 and xanthine. J. Mater. Chem. B 2018, 6, 105–111.
  27. Liu, Y.; Lin, A.; Liu, J.; Chen, X.; Zhu, X.; Gong, Y.; Yuan, G.; Chen, L.; Liu, J. Enzyme-Responsive Mesoporous Ruthenium for Combined Chemo-Photothermal Therapy of Drug-Resistant Bacteria. ACS Appl. Mater. Interfaces 2019, 11, 26590–26606. Ojha, R.P.; Mishra, R.; Singh, P.; Nirala, N.R.; Prakash, R. A composite prepared from MoS2 quantum dots and silver nanoparticles and stimulated by mercury(II) is a robust oxidase mimetic for use in visual determination of cysteine. Microchim. Acta 2020, 187, 74.
  28. Makvandi, P.; Baghbantaraghdari, Z.; Zhou, W.; Zhang, Y.; Manchanda, R.; Agarwal, T.; Wu, A.; Maiti, T.K.; Varma, R.S.; Smith, B.R. Gum polysaccharide/nanometal hybrid biocomposites in cancer diagnosis and therapy. Biotechnol. Adv. 2021, 48, 107711. Zhao, L.; Wang, J.; Su, D.; Zhang, Y.; Lu, H.; Yan, X.; Bai, J.; Gao, Y.; Lu, G. The DNA controllable peroxidase mimetic activity of MoS2 nanosheets for constructing a robust colorimetric biosensor. Nanoscale 2020, 12, 19420–19428.
  29. Ma, J.; Qiu, J.; Wang, S. Nanozymes for Catalytic Cancer Immunotherapy. ACS Appl. Nano Mater. 2020, 3, 4925–4943. Nandu, N.; Salih Hizir, M.; Roberston, N.M.; Ozturk, B.; Yigit, M.V. Masking the Peroxidase-Like Activity of the Molybdenum Disulfide Nanozyme Enables Label-Free Lipase Detection. ChemBioChem 2019, 20, 1861–1867.
  30. Li, Y.; Jia, R.; Lin, H.; Sun, X.; Qu, F. Synthesis of MoSe2/CoSe2 Nanosheets for NIR-Enhanced Chemodynamic Therapy via Synergistic In-Situ H2O2 Production and Activation. Adv. Funct. Mater. 2021, 31, 2008420. Qi, C.; Cai, S.; Wang, X.; Li, J.; Lian, Z.; Sun, S.; Yang, R.; Wang, C. Enhanced oxidase/peroxidase-like activities of aptamer conjugated MoS2/PtCu nanocomposites and their biosensing application. RSC Adv. 2016, 6, 54949–54955.
  31. Mei, L.; Ma, D.; Gao, Q.; Zhang, X.; Fu, W.; Dong, X.; Xing, G.; Yin, W.; Gu, Z.; Zhao, Y. Glucose-responsive cascaded nanocatalytic reactor with self-modulation of the tumor microenvironment for enhanced chemo-catalytic therapy. Mater. Horizons 2020, 7, 1834–1844.
  32. Jiang, H.; Du, Y.; Chen, L.; Qian, M.; Yang, Y.; Huo, T.; Yan, X.; Ye, T.; Han, B.; Wang, Y.; et al. Multimodal theranostics augmented by transmembrane polymer-sealed nano-enzymatic porous MoS2 nanoflowers. Int. J. Pharm. 2020, 586, 119606.
  33. Maji, S.K.; Yu, S.; Chung, K.; Sekkarapatti Ramasamy, M.; Lim, J.W.; Wang, J.; Lee, H.; Kim, D.H. Synergistic Nanozymetic Activity of Hybrid Gold Bipyramid–Molybdenum Disulfide Nanostructures for Two-Photon Imaging and Anticancer Therapy. ACS Appl. Mater. Interfaces 2018, 10, 42068–42076.
  34. Yim, D.; Lee, D.-E.; So, Y.; Choi, C.; Son, W.; Jang, K.; Yang, C.-S.; Kim, J.-H. Sustainable Nanosheet Antioxidants for Sepsis Therapy via Scavenging Intracellular Reactive Oxygen and Nitrogen Species. ACS Nano 2020, 14, 10324–10336.
  35. Chen, T.; Zou, H.; Wu, X.; Liu, C.; Situ, B.; Zheng, L.; Yang, G. Nanozymatic Antioxidant System Based on MoS2 Nanosheets. ACS Appl. Mater. Interfaces 2018, 10, 12453–12462.
  36. Chen, T.; Zou, H.; Wu, X.; Chen, Y.; Situ, B.; Zheng, L.; Yang, G. Fullerene-like MoS2 Nanoparticles as Cascade Catalysts Improving Lubricant and Antioxidant Abilities of Artificial Synovial Fluid. ACS Biomater. Sci. Eng. 2019, 5, 3079–3088.
  37. Ren, C.; Li, D.; Zhou, Q.; Hu, X. Mitochondria-targeted TPP-MoS2 with dual enzyme activity provides efficient neuroprotection through M1/M2 microglial polarization in an Alzheimer’s disease model. Biomaterials 2020, 232, 119752.
  38. Wei, F.; Cui, X.; Wang, Z.; Dong, C.; Li, J.; Han, X. Recoverable peroxidase-like Fe3O4@MoS2-Ag nanozyme with enhanced antibacterial ability. Chem. Eng. J. 2021, 408, 127240.
  39. Cao, F.; Zhang, L.; Wang, H.; You, Y.; Wang, Y.; Gao, N.; Ren, J.; Qu, X. Defect-Rich Adhesive Nanozymes as Efficient Antibiotics for Enhanced Bacterial Inhibition. Angew. Chem. Int. Ed. 2019, 58, 16236–16242.
  40. Wang, T.; Zhang, X.; Mei, L.; Ma, D.; Liao, Y.; Zu, Y.; Xu, P.; Yin, W.; Gu, Z. A two-step gas/liquid strategy for the production of N-doped defect-rich transition metal dichalcogenide nanosheets and their antibacterial applications. Nanoscale 2020, 12, 8415–8424.
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