2. Applications Oriented Studies from Laboratory to Pilot Scale
Conventional suspensions prepared from phytocompounds have antifungal effects. The antifungal range of a nanoemulsion made by ultrasonication using
Cleome viscosa essential oil and Triton-x-100 was studied. Essential oil nanoemulsion (EONE) was evaluated with foodborne pathogenic
Candida albicans at a minimum inhibitory and fungicidal dosage. The MIC and MFC values for
C. albicans isolates ranged from 16.5 to 33 mL/mL, with a considerable reduction in biofilm. Fourier transformed infrared spectroscopy corroborated the shift in compositional fingerprinting, and spectroscopic analysis revealed a drop in chitin levels in cell walls. In
C. albicans cells, EONE and its biologically active compounds cause massive damage
[15][105].
Several techniques have proven that the primary components of EOs have antioxidant, antibacterial, and antifungal effects. Tea tree oil, lemon oil, cinnamon oil, clove oil, and thyme oil, among other EOs from local plants, have positively influenced antibacterial and antioxidant activity, along with expanded cereal shelf lives and enhanced food security. In addition, terpenes and volatile aromatic chemicals, for example, are important EO categories that help food hygiene without affecting quality. For example, EOs might be utilized as an additional preservative to extend the shelf life of grains and cereals because of their numerous effects, including antioxidant and antibacterial properties
[16][106].
The antifungal and anti-aflatoxigenic activities of 5′-hydroxy-aurapten (5′-HA) on
A. flavus isolated from nuts (
Lotus lalambensis) were investigated. In this study, 5′-HA demonstrated a higher antifungal potential against
A. flavus, having a minimum inhibitory concentration of 62.5 mg/L. It was found that 5′-HA had reduced conidia germination for
A. flavus by 60% at a dose of 40 mg/mL in the early (A, B, C), middle (L, M, N), and late (P, Q, W) stages of the aflatoxin biosynthesis pathway. Moreover, 5′-HA also inhibited the synthesis of aflatoxins, AFB1 and AFB2, by 50% and 23.3%, respectively. 5′-HA increased the efficacy of enzymatic antioxidants CAT (Catalase) and SOD (Superoxide dismutase) by 56.25% and 66.66%, respectively. The anti-aflatoxigenic mechanism of 5′-HA is thought to work by increasing the expression profile of the transcription factors
atfA and
atfB by 2- and 2.5-fold, respectively
[17][107]. Sodium lignosulfonate was found to be an antifungal compound due to its fungistatic activity against
M. circinelloides,
A. amoenus, and
P. solitum. These strains were obtained from spoiled alfalfa hay (
Medicago sativa). Sodium lignosulfonate (NaL) had superior preservation properties for the ground high-moisture hay as a substrate
[18][108]. In comparison to spoiled hay, sodium lignosulfonate and PRP had a protective effect against hay proteolysis at a concentration of 0.5%, as assessed by a decrease in ammoniacal nitrogen (NH
3-N). Preservatives can prevent plant proteins from deteriorating, retaining their biological worth, according to these studies.
Natamycin is an antifungal medicine with poor solubility that is used in food products to address the base of cheese and sausages. This use does not risk the customer’s safety. For beverage preservation, a highly soluble natamycin–cyclodextrin integral membrane was created. This approach results in high drug concentrations that are dangerously above the acceptable limit. In addition to assessing an adequate daily natamycin food intake, researchers must investigate natamycin’s impact on the intestinal bacteria as a reservoir for tolerance, which results from the amount of feces in one’s system to be abnormally high. Foods having natamycin, introduced and blended uniformly, such as yoghurt, and even the administration of cyclodextrin intercalation to drinks and wine, all contribute to natamycin levels and fecal
Candida spp. drug exposure.
Candida spp. have established natamycin tolerance in the bowels of persons who have been treated with natamycin for fungal diseases. As a consequence, it is impossible to figure out the likelihood that using natamycin to keep yoghurt and beverages promotes
Candida spp. polyene tolerance
[19][109].
The bioactivity of
Lactobacillus brevis AM7 during fermentation with bread hydrolysate was evaluated against the fungus (20% to 70%). Using Liquid Chromatography, nine antifungal compounds (with 10–17 amino acid residues and masses spanning 1083.6 to 1980.7 Da) were investigated, all of which were expressed in wheat protein sequences. Bread hydrolysate fermented by
L. brevis AM7, non-fermented bread hydrolysate, and a slurry composed of water and bread combination were all used to make bread and compared with conventional wheat bread. Compared to the other pieces of bread, those fermenting hydrolysate (18 and 22% of the dough weight) had the maximum mold-free shelf life, extending up to 10 days until mold appeared. Moreover, the fermentation hydrolysate had the fewest adverse influences on bread texture, demonstrating biotechnology’s beneficial impact and potential
[20][22]. The essential oil of
Thymus algeriensis was studied as a possible soft cheese preservative. We devised a novel method for determining the essential oil’s ability to preserve soft cheese. During 30 days of storage at 4 °C with 25 L of essential oil introduced, there was no contamination of
Penicillium aurantiogriseum. Minimum inhibitory concentrations for antifungals varied from 0.01 to 0.04 mg/mL range. According to the data, the oil was active with a half-maximal inhibitory activity of 0.132 mg/mL. The volatile components in the oil were determined by using gas chromatography, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectrometry. The most frequent constituent in the oil was discovered to be carvacrol, which made up 80.9% of the overall amount, followed by p-cymene (7.7%)
[21][110].
Both people and the environment are put at risk by chemical preservatives and fungicides. Bio-preservatives, such as lactic acid bacteria (LAB), on the other hand, are efficient, secure, and biodegradable, as well as add adequate beneficial health effects. The antifungal activity of strain RM1 was the highest amongst 23 rod-shaped LAB isolates collected from Egyptian traditionally fermented milk (Rayeb). Strain RM1 was distinguished from genetically similar Lactobacillus species by 16S rRNA phylogenetic analysis and distinctive phenotypic traits, indicating that it is a distinct species whereby the name Lactobacillus sp. RM1 is suggested. Lactobacillus sp. RM1 cell-free supernatant (CFS) has considerable and broad antifungal effects, mostly against toxigenic fungi and pathogenic bacteria.
Lactobacillus spp. RM1 has antifungal capabilities and the ability to prolong the shelf life of wheat grains, implying that it could be used as a natural food preservative
[22][65]. Antimicrobial substances generated or expelled by LAB can counteract foodborne illnesses, making it a possible alternative to artificial preservatives
[23][111]. Natural preservatives such as LAB are effective, safe, and biodegradable, with added health advantages. LAB is also frequently used as a bio-preservative to increase the shelf life of food products while in storage
[24][25][112,113]. Organic acids, short-chain fatty acids, hydrogen peroxide, reuterin, diacetyl, bacteriocins, and bacteriocin-like inhibitory compounds are some of the antifungal substances produced by LAB
[26][22][27][19,65,114],
Lactococcus lactis spp.
lactis ATCC 19435 inhibits fungal growth and ochratoxin A synthesis in fungal growth conditions
[28][29][115,116]. Antifungal compounds found in LAB have been proven effective in decreasing yeasts and molds that degrade food
[30][117]. Therefore, to eliminate toxic fungus and increase the quality, safety, and shelf life of food and agricultural products, it is critical to look for natural, food-grade antifungal chemicals from LAB.