Fish Exposed to Ammonia Nitrogen: Comparison
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Ammonia nitrogen is the major oxygen-consuming pollutant in aquatic environments. Exposure to ammonia nitrogen in the aquatic environment can lead to bioaccumulation in fish, and the ammonia nitrogen concentration is the main determinant of accumulation. In most aquatic environments, fish are at the top of the food chain and are most vulnerable to the toxic effects of high levels of ammonia nitrogen exposure. In fish exposed to toxicants, ammonia-induced toxicity is mainly caused by bioaccumulation in certain tissues. Ammonia nitrogen absorbed in the fish enters the circulatory system and affects hematological properties. Ammonia nitrogen also breaks balance in antioxidant capacity and causes oxidative damage. In addition, ammonia nitrogen affects the immune response and causes neurotoxicity because of the physical and chemical toxicity. 

  • ammonia nitrogen
  • fish
  • oxidative stress
  • neurotoxicity
  • immune response

1. Introduction

Ammonia nitrogen is the final product of protein catabolism and metabolism. In farming water, ammonia nitrogen is mainly derived from the decomposition process of organic matter. It is the most common environmental limiting factor in aquaculture [1]. The ammonia concentration in naturally occurring water is approximately 0–0.2 mg/L, which is at a low level. However, with the development of culturing systems, the excessive concentration of ammonia nitrogen in farmed water has become a common environmental problem. Ammonia has the greatest impact on the physiological function of aquatic organisms. Ammonia can accumulate quickly and reach toxic concentrations [2]. There are two main reasons for the growth of the mass concentration of ammonia nitrogen in farming water: one is the decomposition of organic matter containing nitrogen such as feed residues and aquatic animal excreta [3]. High-density farming increases the burden of these substances, resulting in the accumulation of ammonia nitrogen. The second is industrial wastewater, domestic sewage discharge, etc. In various regions of fertilization of pesticides agriculture, the massive use of nitrogen fertilizers, mainly ammonium chloride and urea, entering the farming water with the washout of rainwater and surface runoff [4].
Ammonia nitrogen is the sum of ammonia present in water in two forms of ionized (NH4+) and non-ionic ammonia (NH3), which is also called total ammonia (TAN) [5]. The equilibrium reaction equation of both is
Ammonia nitrogen is the main product of fish metabolism, and most fish are sensitive to ammonia [6]. The increase of non-ionic ammonia in the water environment will inhibit the excretion of ammonia nitrogen in the fish and increase the concentration of ammonia in their blood and tissues, making the blood less capable of carrying oxygen and disrupting normal metabolism. Non-ionic ammonia is the main toxic form of physiological stress in fish and is about 300 to 400 times more toxic than ionized ammonia [4]. Non-ionic ammonia has good lipid solubility and carries no electric charge, making it more easily fuse with the phospholipid bilayer on the cell membrane, thus diffusing through the cell membrane to the hemolymph and increasing the concentration of ammonia nitrogen in fish, producing toxic effects [7]. Furthermore, non-ionic ammonia has good lipid solubility. It can easily diffuse into fish through cell membranes, causing damage to important organs, including gill tissue, resulting in respiratory difficulties and reduced feeding rate of fish, and inhibiting their growth and development [8]. The non-ionic ammonia which enters the fish directly affects the metabolism of enzymes, causing disorders of enzyme metabolism and reducing immunity, stimulating a series of toxic reactions in fish, such as excitement and convulsions until they die of exhaustion [9,10][9][10].
We comprehensively analyzed the main toxicological characteristics and physiological reaction processes of fish after exposure to ammonia (Figure 1). The toxicity of ammonia nitrogen is manifold, and therefore it is necessary to conduct an overall research and analysis to confirm the adverse effects of ammonia nitrogen on fish. Thus, the aim of this review was to summarize changes in ammonia accumulation patterns, oxidative stress, neurotoxicity and changes in immunological reactions in fish following exposure to ammonia nitrogen.
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Figure 1. Toxicological characteristics and physiological response process for fish exposed to ammonia [11]. AMM: ammonia; COR: cortisol; SOD: superoxide dismutase; CAT: catalase; GSH: glutathione; GST: Glutathione S-transferase; TBARS: Thiobarbituric reactive substances; MDA: malon dialdehyde; RBC: red blood cell count; Hb: hemoglobin; Ht: hematocrit; GLU: glucose; AST: aspartate transaminase; ALT: alanine transaminase; ALP: alkaline phosphatase; Ils: interleukins; TNFs: tumor necrosis factors; FER: feed efficiency ratio; SGR: specific growth—Reprinted from Ref. [11].

2. Bioaccumulation

The toxicity of ammonia to fish mostly results from bioaccumulation as well as excretion, metabolism and detoxification mechanisms caused by ammonia nitrogen uptake [12]. In water, ammonia is present as non-ionic ammonia (NH3) and ionized ammonium (NH4+), the latter accounting for a large proportion at normal water pH [3]. Most biofilms are not permeable to ammonium ions but are permeable to ammonia. Thus, the ammonia toxicity is owing to its non-ionic form (NH3), which can easily spread through gill membranes [13]. Furthermore, provided there is an outward gradient, the ammonia can be discharged into water as NH3 through gills, and this process is aided by Rhesus (Rh) glycoproteins [14]. However, under high ambient ammonia (HEA), the external ammonia flux through gills is decreased and the inverse internal flux of ammonia occurs. Therefore, ammonia levels in blood and tissues increased and acute and chronic toxic reactions were observed in fish [15,16][15][16].
Ammonia toxicity to fish has been demonstrated to follow a multifactorial pathogenesis. It is generally considered that NH3 enters the organism through the gills, epidermis, and intestinal mucosa of fish, which increases the blood pH and reduces oxygen carrying capacity of blood. Long-term exposure to ammonia also damages gill tissue, leading to gill tissue congestion, gill lamellae bending and adhesion, affecting gill gas exchange, inhibiting the respiratory function of the organism, leading to fish hypoxia and even death [7]. After ammonia diffuses into the tissues, the ammonia level increases [17], damaging the liver and kidney system, resulting in congestion, edema, liver coma and even death [18].
Ammonia can also damage the central nervous system of fish. Initially, researchers believed that mechanisms of ammonia poisoning of fish were similar to those of hepatic encephalopathy in mammals [19]. This is because high levels of non-ionic ammonia (NH3) in the brain synthesize high amounts of glutamine (Gln) catalyzed by glutamine synthetase, swelling neuroglia and activating the N-methyl-D-aspartate (NMDA) receptor [20]. Under normal conditions, the organism produces Gln mainly from glutamate (Glu) and NH4+ to remove excess NH4+ from the brain. Therefore, increased concentration of NH4+ in the brain is often accompanied by an increase in the level of Gln. While the excessive increase in Gln intracellularly leads to astrocyte swelling [21], triggering brain edema and promoting the release of Glu into the intercellular, causing intracranial hypertension and death [22]. In addition, NH4+ also causes depolarization of the neuronal surface. Both Glu and depolarized neurons accumulated intercellularly can activate NMDA receptors located on the neuronal surface. Excessive activation of this receptor promotes NO synthesis and activates Na+/K+-ATPase [23,24][23][24]. Activation of Na+/K+-ATPase accelerates energy depletion in the brain. The excessive amount of NO not only increased the oxidative stress in the organism, but also tends to produce extremely toxic hydrogen peroxide, which impairs the mitochondrial respiration, initiates ATP exhaustion and results in cell death [25,26][25][26]. This assumption has been accepted by most scholars for a long time. In the early 21st century, several studies confirmed that some fish, such as Cyprinus and mudskipper, accumulate glutamine after ammonia stress at concentrations much greater than those that constitute lethal concentrations in mammals [27,28][27][28]. The histological data suggested that the skull of fish has more cranial space than mammals and they can bear more cranial pressure [11]. Therefore, fish may have different defense mechanisms to ammonia toxicity than mammals.
Nevertheless, physical stress responses (or pathological processes) could be triggered by the production of metabolites in adaptive regulation, for example, the oxidative damage and inflammatory hyperinflammation, which could be the real reason for fish death [29]. Likewise, Hegazi et al. indicated that ammonia nitrogen stress triggers the formation of reactive oxygen species, which are also mediators of ammonia toxicity [30]. In recent years, with the continuous innovation of research methods in aquatic animals, new evidence suggests that ammonia toxicity in fish is associated with the over inflammatory response of the organism [31,32][31][32]. The exact mechanisms of ammonia toxicity to fish need to be further examined.

3. Hematological Parameters

The inflow of toxic materials to the water environment affects water parameters and leads to alterations in the fish hematological profile [33,34][33][34]. Toxic exposure can adversely affect the blood oxygen carrying capacity and the blood electrolyte balance, especially ammonia exposure can induce the accumulation of ammonia in fish circulatory system. As ammonia has high affinity for blood hemoglobin, it displaces oxygen and influences some hematological properties [35]. Hematological properties are significant indicators to assess fish health status after exposed to different environmental stresses and chemical toxicity [36,37,38,39][36][37][38][39]. Ammonia enters the fish circulatory system and causes metabolic disturbances and fatal responses, such as oxidative stress, immune response and genetic expression [40]. Exposure to ammonia also affects the fish circulatory system, particularly hematological indexes such as red blood cell count (RBC), hematocrit (Ht) and hemoglobin (Hb) [32]. In general, exposure to toxic substances decreases hematological properties like RBC, Ht and Hb owing to the hemolysis and red blood cells destruction and may cause anemia [41]. Praveena et al. suggested that the decrease in RBC and the concentration of hemoglobin resulting from toxicity exposure might be attributed to the destructive effects of toxicity, but the decrease in the concentration of hemoglobin resulted in a potential impairment of tissue function because of the inadequate oxygen supply to the tissues [42]. Gao et al. observed significant declines in Hb content, Ht and blood RBC counts after exposure to high concentrations of ammonia in Takifugu rubripes, suggesting that the fish was anemic by exposure to ammonia [40]. Hoseini et al. revealed that the increase in radicals exposed to ammonia could lead to attack on RBCs, leading to their destruction [43]. Researches have revealed that the ammonia toxicity induces the inhibition of hematopoiesis by destroying the production sites of red blood cells [44]. The onset of anemia symptoms may be due to destruction of red blood cells or injury to hematopoietic tissues after exposure to ammonia. Serum proteins are considered to be reliable indicators of fish immune status and health [45]. David et al. attributed the decreased protein content in the toxically exposed fish to the disruption or collapse of cell functions and the concomitant impairment of protein synthetic mechanisms [46]. Asthana et al. reported that high concentration of ammonia resulted in the deamination of proteins and increased the degradation of proteins [47]. Ammonia exposure induced a reduction in hematological properties like RBC, Ht and Hb in fish, which is considered to account for the stress-induced decline in Hb content and Hb synthesis rate. Thus, it may exert a toxic effect by inducing disturbances in tissue oxygenation.
Ammonia absorbed in fish diffuses through cell membranes into the blood system and causes accumulation. Therefore, hematological parameters usually act as sensitive indicators to evaluate the toxicity of ammonia to fish [48]. The effects of ammonia on fish hematological parameters are shown in Table 1, regarding the route of exposure (freshwater, seawater, waterborne exposure). The decrease in hematological properties induced by ammonia exposure is manifested by disruption of RBC and alteration of the small or large red blood cell anemic state. Das et al. reported changes in blood characteristics (such as RBCs and hemoglobin) of Cirrhinus mrigala after exposure to ammonia [49]. This phenomenon causes tissue damage due to ammonia toxicity and hemodilution after hemolysis. Iheanacho et al. showed that changes in RBC content, hematocrit values and hemoglobin concentration reflected the fish defensive mechanisms against stress induced by exposure to environmental toxicity [50]. Kim et al. revealed that the ammonia exposure greatly decreased the levels of hematocrit and hemoglobin in juvenile hybrid grouper [48]. These authors considered that fish tissues might be under hypoxic conditions because of ammonia exposure and may result in the inhibition and depletion of hematopoietic potential under that condition.
Table 1. Hematological parameters in fish exposed to ammonia.
Exposure Route Exposure Type Fish Specie Ammonia

Concentration		 Exposure Time Response

Concentration		 Response * Reference
RBC (Billion/mL)
Sea water Waterborne

exposure		 Takifugu rubripes 5, 50, 100, 150 mg/L 24, 48, 96 h 100, 150 mg/L − Gao et al. [40] 2021
Freshwater
81][66]. 2021 Jun-Hwan Kim, Young-Bin Yu, Jae-Ho Choi.
Table 2 shows the responses of antioxidant enzymes in fish exposed to ammonia. The oxidative stress in fish exposed to ammonia stress is indicated by changes in the production of ROS in fish. One of the main defense strategies to reduce ROS production is by raising the activity of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) [35,65,66,67,68,69][35][65][67][68][69][70]. Antioxidant enzymes are widely present in tissues and are most abundant in hepatocytes [70,71][71][72]. Under stress, fish can protect the structure and function of cell membranes from peroxides by converting O2− to H2O2 through SOD, GSH and CAT and by breaking down cytotoxic H2O2 into oxygen and water [72][73].
Table 2. Antioxidant enzyme responses such as SOD, CAT and GST in fish exposed to ammonia.
Exposure Route Exposure

Type		 Fish Specie Ammonia

Concentration		 Exposure Periods Response

Concentration		 Target Organs Response * Reference
SOD (Superoxide dismutase)
Sea water Waterborne

exposure		 Dicentrarchus labrax 20 mg/L 12, 48, 84, 180 h 20 mg/L Blood × Sinha et al. [76][74] 2015
Epinephelus fuscoguttatus ♀ Waterborne

exposure		 Cyprinus carpio 106mg/L 24 h 106 mg/L − × E. lanceolatus ♂Hoseini et al. 1, 2, 4, 8 mg/L[ 1week, 2 wk 4, 8 mg/L Liver, Gill + Kim et al. [48] 202043] 2019
Megalobrama amblycephala 5, 10, 15, 20 mg/L 9 weeks 20 mg/L − Zhang et al. [11] 2019
Scophthalmus maximus 5, 20, 40 mg/L 24, 48, 96 h 20, 40 mg/L Ht (%)
Liver + Jia et al. [98][75] 2020
Chlamys farreri 20 mg/L 1, 12, 24 d 20 mg/L Blood + Wang et al. [85][76] 2012 Sea water Waterborne

exposure		 Takifugu rubripes 5, 50, 100, 150 mg/L
Takifugu rubripes 5, 50, 100, 150 mg/L24, 48, 96 h 24 h50, 100, 150 mg/L − 50, 100, 150 mg/L Gill + Gao et al. [40]Gao et al. [40] 2021
2021 Piaractus

mesopotamicus		 1, 2, 3 mg/L 96 h 2, 3 mg/L + Edison et al. [57][51] 2015
Freshwater
48, 96 h 50, 100, 150 mg/L Gill − Waterborne

exposure		 Cyprinus carpio 106 mg/L 24 h 10 6mg/L − Hoseini et al. [43] 2019
Freshwater Waterborne

exposure		 Carassius auratus 10, 50 mg/L 30 d 10, 50 mg/L Liver − Qi et al. [9] 2017 Megalobrama amblycephala 5, 10, 15, 20 mg/L 9 weeks 20 mg/L −
Megalobrama amblycephala 5, 10, 15, 20 mg/LZhang et al. 9 weeks 20 mg/L Liver[11] 2019
− Zhang et al. [11] 2019 Hb (g/L)
Cyprinus carpio 106 mg/L 24 h 106 mg/L Blood × Hoseini et al. [43] 2019 Sea water Waterborne

exposure		 Takifugu rubripes 5, 50, 100, 150 mg/L 24, 48, 96 h 50, 100, 150 mg/L − Gao et al. [40] 2021
Oreochromis niloticus 5, 10 mg/L 70 days 5, 10 mg/L Liver, Muscle + Hegazi et al. [87][77] 2010 Piaractus

mesopotamicus		 1, 2, 3 mg/L 96 h
CAT (Catalase)2, 3 mg/L − Edison et al. [57][51] 2015
Freshwater Waterborne

exposure		 Cyprinus carpio 106 mg/L 24 h 106 mg/L − Hoseini et al. [43] 2019
Sea water Waterborne

exposure		 Dicentrarchus labrax 20 mg/L 12, 48, 84, 180 h 20 mg/L Blood + Sinha et al. [76][74] 2015 Megalobrama amblycephala 5, 10, 15, 20 mg/L 9 weeks 20 mg/L − Zhang et al. [11] 2019
Scophthalmus maximus 5, 20, 40 mg/L 24, 48, 96 h 20, 40 mg/L Liver + Jia et al. [ Glucose (mg/dL)
98][75] 2020
Takifugu rubripes 5, 50, 100, 150 mg/L 24 h 50, 100, 150 mg/L Gill + Gao et al. [40] 2021 Sea water Waterborne

exposure		 Takifugu rubripes 5, 50, 100, 150 mg/L 24, 48, 96 h 50, 100, 150 mg/L + Gao et al. [40] 2021
48, 96 h 50, 100, 150 mg/L Gill − Litopenaeus vannamei 0.32, 0.44, 0.60 mg/L 6 h, 12 h, 1 day, 2 days 0.32, 0.44, 0.60 mg/L + Cui et al.
Freshwater Waterborne

exposure		[53] Carassius auratus 10, 50 mg/L[ 30 days52] 2017
10, 50 mg/L Liver × Qi et al. [9] 2017 Piaractus

mesopotamicus		 1, 2, 3 mg/L 96 h 2, 3 mg/L + Edison et al. [57][51] 2015
Freshwater Waterborne

exposure		 Pelteobagrus fulvidraco 100 mg/L 24, 48, 72 h 100 mg/L + Zhao et al. [52][53] 2021
Megalobrama amblycephala 5, 10, 15, 20 mg/L 9 weeks 20 mg/L − Zhang et al. [11] 2019
Cyprinus carpio 0.5 mg/L 24 h 0.5 mg/L + Mirghaed et al. [58][54] 2019
Total protein (g/dL)
Sea water Waterborne

exposure		 Takifugu rubripes 5, 50, 100, 150 mg/L 24, 48, 96 h 50, 100, 150 mg/L − Gao et al. [40] 2021
Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂ 1, 2, 4, 8 mg/L 1week, 2 weeks 8 mg/L − Kim et al. [48] 2020
Freshwater Waterborne

exposure		 Pelteobagrus fulvidraco 100 mg/L 24, 48, 72 h 100 mg/L + Zhao et al. [52][53] 2021
Inject Ctenopharynodon idellus 9 μL 96 h 9 μL × Xing et al. [59][55] 2016
AST (U/L)
Freshwater Waterborne

exposure		 Pelteobagrus fulvidraco 100 mg/L 24, 48, 72 h 100 mg/L + Zhao et al. [52][53] 2021
Megalobrama amblycephala 5, 10, 15, 20 mg/L 9 weeks 20 mg/L + Zhang et al. [11] 2019
Cyprinus carpio 106 mg/L 24 h 106 mg/L + Hoseini et al. [43] 2019
ALT (U/L)
Sea water Waterborne

exposure		 Takifugu rubripes 5, 50, 100, 150 mg/L 24, 48, 96 h 50, 100, 150 mg/L + Gao et al. [40] 2021
Freshwater Waterborne

exposure		 Pelteobagrus fulvidraco 100 mg/L 24, 48, 72 h 100 mg/L + Zhao et al. [52][53] 2021
Megalobrama amblycephala 5, 10, 15, 20 mg/L 9 weeks 20 mg/L × Zhang et al. [11] 2019
Cyprinus carpio 106 mg/L 24 h 106 mg/L + Hoseini et al. [43] 2019
ALP (U/L)
Freshwater Waterborne

exposure		 Pelteobagrus fulvidraco 100 mg/L 24, 48, 72 h 100 mg/L × Zhao et al. [52][53] 2021
Megalobrama amblycephala 5, 10, 15, 20 mg/L 9 weeks 20 mg/L × Zhang et al. [11] 2019
Cyprinus carpio 106 mg/L 24 h 106 mg/L + Hoseini et al. [43] 2019
* +: increase, −: decrease, ×: no effect.
The balance of glucose levels is maintained by balancing the production of glucose and the storage of glucose as glycogen [51][56]. Glucose metabolism meets the energy requirements of the organs and tissues, which can mediate the ammonia response. Zhao et al. reported a significant increase in glucose in juvenile yellow catfish, Pelteobagrus fulvidraco, exposed to ammonia [52][53]. Long-term exposure to ammonia results in a dramatic elevation of blood glucose in Litopenaeus vannamei owing to the impaired glucose metabolism in the liver [53][52]. Lower glucose levels were critical for reducing tissue injuries and maintaining low levels of gene expression of pro-inflammatory cytokines in stressful conditions [32]. Changes in glucose levels in fish caused by ammonia exposure were attributed to stress responses or disturbances in homeostasis.
Enzyme plasma components including aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) are recognized as credible and sensitive biological indicators for evaluating damages to the liver and other fish organs following environmental stress [54][57]. Plasma ALT and AST levels play important roles in indicating hepatopancreatic functions and injuries, and can be used as sensitive indicators of hepatocyte integrity [55][58]. Zhao et al. revealed that AST and ALT levels were significantly increased in juvenile yellow catfish, Pelteobagrus fulvidraco, exposed to ammonia, which may be due to damage to cell membranes and liver [52][53]. Hoseini et al. recorded significant increases in ALT, AST and ALP of Cyprinus carpio following ammonia exposure, which may be due to damage to cell membranes [43]. Zhang et al. reported that ALP levels were significantly increased in Megalobrama amblycephala exposed to ammonia [11]. ALP is an important indicator reflecting liver damage, so the upward trend in ALP in Megalobrama amblycephala is thought to be attributed to liver damage and stress caused by ammonia exposure. Peyghan et al. reported that ammonia exposure induced a remarkable increase in ALP in Cyprinus carpio, indicating that hematological parameters were affected [56][59].
Ammonia enters the circulation and disrupts blood proteins participated in lipid metabolism, immune defense, blood coagulation and molecular transport, when fish are exposed to ammonia [11,13][11][13]. Specifically, ammonia particles may affect various blood physiologies through chemical and physical interactions in the blood. Various studies have confirmed changes in various hematological properties following ammonia exposure, and hematological parameters may be a reliable indicator for assessing fish toxicity.

4. Oxidative Stress

Oxidative stress is one of the toxicity mechanisms of ammonia nitrogen stress in aquatic animals [7]. It has been shown that the increase in the concentration of ammonia nitrogen in aquaculture water can result in the production of reactive oxygen species (ROS) in aquatic animals [60]. ROS combines with unsaturated fatty acids and cholesterol on the cell membrane to produce lipid peroxidation, which leads to reduced mobility and greater cell membrane permeability. Disturbance of the distribution of proteins across the cell membrane leads to cell membrane dysfunction and apoptosis [61,62][61][62]. In order to counteract antioxidant stress and maintain the balance of the redox state of cells, antioxidant defense systems have evolved to function at different levels to avoid or repair this damage [31]. The mechanism of oxidative stress in fish exposed to ammonia is shown in Figure 2. Studies have reported that the activities of antioxidant enzymes can be elicited in low concentrations of pollutants and disrupted in high concentrations [63,64][63][64]. When physiological antioxidant system is unable to counteract the increased levels of stress-generated ROS, cellular oxidative stress occurs [65].
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Figure 2. Oxidative stress mechanisms in fish exposed to ammonia [81][66]. H2O2: hydrogen peroxide; SOD: superoxide dismutase; CAT: catalase; GSH: glutathione; GST: Glutathione S-transferase; TBARS: Thiobarbituric reactive substances; MDA: malon dialdehyde; GPx: glutathione peroxide; GR: glutathione reductase; GSSG: glutathione; NADPH: nicotinamide adenine dinucleotide phosphate—Reprinted with permission from Ref. [
Megalobrama amblycephala
5, 10, 15, 20 mg/L
9 weeks
20 mg/L
Liver
−
Zhang et al.
[
11
]
2019
Cyprinus carpio 106 mg/L 24 h 106 mg/L Blood − Hoseini et al. [43] 2019
Corbicula fluminea 10, 25 mg/L 24, 48 h 10 mg/L Digestive gland + Zhang et al. [84][78] 2020
10, 25 mg/L 24, 48 h 10 mg/L Gill ×
10, 25 mg/L 24, 48 h 25 mg/L Digestive gland −
10, 25 mg/L 24, 48 h 25 mg/L Gill +
GST (Glutathione-S-transferase)
Sea water Waterborne

exposure		 Dicentrarchus labrax 20 mg/L 12, 48, 84, 180 h 20 mg/L Blood + Sinha et al. [76][74] 2015
Takifugu rubripes 5, 50, 100, 150 mg/L 24 h 50, 100, 150 mg/L Gill + Gao et al. [40] 2021
48, 96 h 50, 100, 150 mg/L Gill −
Epinephelus fuscoguttatus♀ × E. lanceolatus ♂ 1, 2, 4, 8 mg/L 1 week 4, 8 mg/L Liver, Gill + Kim et al. [48] 2020
Epinephelus fuscoguttatus♀ × E. lanceolatus ♂ 1, 2, 4, 8 mg/L 2 weeks 4, 8 mg/L Liver, Gill −
Freshwater Waterborne

exposure		 Carassius auratus 10, 50 mg/L 30 days 10, 50 mg/L Liver × Qi et al. [9] 2017
Paralichthys orbignyanus 5, 10 mg/L 70 days 5, 10 mg/L Liver, Muscle + Hoseini et al. [43] 2019
Cyprinus carpio L. 10, 20, 30 mg/L 6, 24, 48 h 30 mg/L Liver + Li et al. [10] 2019
10, 20, 30 mg/L 6, 24, 48 h 10, 20, 30 mg/L Gill +
* +: increase, −: decrease, ×: no effect.
SOD is first defense against antioxidant stress [73][79]. As a primary defense mechanism against antioxidant stress, SOD transforms superoxide radicals into hydrogen peroxide (H2O2). SOD activity in ammonia-exposed fish is usually increased due to defense mechanisms against ROS production [74][80]. Changes in SOD concentration appear to be associated with differences ammonia tolerance of fish. The SOD activity of rainbow trout, carp, goldfish and Dicentrarchus labrax did not change significantly under ammonia stress [75,76][81][74]. SOD activity decreased significantly in Carassius auratus and Litopenaeus vannamei [9,77][9][82]. While in Takifugu obscurus and Pelteobagrus vachelli, SOD activity increased significantly [78,79][83][84]. However, excessive accumulation of free radicals inhibits antioxidant enzyme capacity to scavenge ROS, and a significant downward trend in SOD activity is observed after a remarkable increase in SOD activity. Kim et al. found that SOD activity in the liver and gills significantly increased when juvenile hybrid grouper exposure to ammonia. However, SOD activity in the gills had a downward trend when fish was subjected to high levels of ammonia. [48]. Sun et al. revealed that when bighead carps were exposed to high concentrations of ammonia, the SOD activity of bighead carp, Hypophthalmythys nobilis, larvae first increased and then decreased [80][85]. The SOD activity may be stimulated in response to excessive ROS production, but then SOD activity declines because they are unable to perform under higher ammonia concentrations. Ammonia exposure also leads to reduction in antioxidant enzymes as the energy expended in response to antioxidant stress. CAT is the major antioxidant enzyme to eliminate H2O2, a by-product of SOD, thus reducing its toxic effects [35,69,82][35][70][86]. Xue et al. showed that ammonia exposure can depress normal ROS-mediated oxidative processes and reported a reduction of CAT activity in Cyprinus carpio following ammonia exposure [83][87]. Zhang et al. reported that ammonia exposure decreases SOD and CAT in the digestive gland of Corbicula fluminea after an initial increase. This phenomenon is thought to be caused by the inhibition of antioxidant enzymes, as the ROS generated in the tissues are not cleared immediately. The activity of CAT also declined with increasing the concentration of ammonia, which indicated the oxidative damage and stress [84][78]. In addition, Wang et al. concluded that the short time increase in antioxidant enzyme activity with treatment was not sufficient to fully counteract stress-induced cellular damage [85][76]. In normal conditions, ROS are quickly removed by the antioxidant defense system. However, stimulated by large amounts of ammonia, excess ROS were produced, disrupting the cell membrane, forming lipid peroxides and oxidized proteins, and the balance between oxidants and antioxidants was disrupted. The body’s detoxification function was severely inhibited. Glutathione S-transferase (GST) functions in the second stage of the fish detoxification metabolism by conjugating to xenobiotics and clearing them from the cells, and the activity of GST is usually triggered in fish exposed to environmental toxins [69][70]. Thus, GST plays a key role in homeostasis and foreign body dissociation, protecting tissues from oxidative stress of toxicant exposure [86][88]. Many scholars have reported that ammonia exposure affects GST activity in fish through the induction of oxidative stress [10,75,87][10][81][77]. Maltez et al. suggested that GST activity in Brazilian flounder (Paralichthys orbignyanus) increased as a result of ammonia exposure and that ROS increased by ammonia exposure stimulated antioxidant defense [88][89]. Kim et al. reported that GST activity in juvenile hybrid grouper initially increased significantly and tended to decrease with higher ammonia exposure concentration and time [48]. Li et al. also reported a significant upward trend and subsequent downward trend in GST activity in carp following exposure to acute ammonia gas [10]. The decrease in GST after the initial increase may be caused by excessive ROS production, which is consistent with the changes observed in SOD activity. Thiobarbituric reactive substances (TBARS) was used as a measure of lipid peroxidation [89][90]. It is well known that oxidative stress is caused by lipid peroxidation, leading to loss of cellular function [90][91]. TBARS is a sensitive indicator for estimating lipid peroxidation as its products are produced by peroxidation of membrane lipids [91][92]. Li et al. reported a gradual rise in brain TBARS levels in Pelteobagrus fulvidraco exposed to high ammonia. They suggested that elevated brain TBARS levels could be an essential factor in the pathogenesis of ammonia toxicity in fish exposed to high concentrations of ammonia [92][93]. Almroth et al. suggested that lipid peroxidation in fish occurs during oxidative stress from environmental toxicants [93][94]. Aldehydes and ketones scan to crosslink with nucleophilic moieties of proteins, nucleic acids and aminophospholipids, and greater levels of TBARS result in increased cytotoxicity and accelerated cell and tissue damage [94][95]. In particular, as fish contain many highly unsaturated fatty acids (HUFA), TBARS can be used as a biomarker of oxidative stress [95][96]. Excessive ROS production induces oxidative damage when fish were exposed to toxic substances, such as ammonia nitrogen. Excess ROS may damage cell membranes, form lipid peroxides and oxidize proteins [33]. Usually, MDA is used as a biomarker of lipid peroxidation because it is an important product of membrane lipid peroxidation following free radical attack on biological membranes [96,97][97][98]. The changes of MDA content can indirectly reflect the level of disruption of biofilm system. Li et al. found no significant changes in the MDA content of Cyprinus carpio L. during exposure to 10 mg/L ammonia water. However, after 48 h of exposure to 30 mg/L ammonia, MDA levels increased significantly [10]. Xue et al. found that MDA levels had an upward trend when Cyprinus carpio exposed to ammonia [83][87]. The glutathione redox system could be triggered by ammonia stress. However, the antioxidant reaction is not sufficient to prevent oxidative damage caused by increased ammonia concentration. Ammonia exposure has toxic effects on fish through the induction of oxidative stress and ROS production, and antioxidant enzymes in fish such as SOD, CAT, GST, TBARS and MDA are the main indicators to reflect oxidative stress caused by ammonia.

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