Five Rosmarinus Essential Oils: Comparison
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The present study reported the investigation of the chemical profile and the extraction yield of the essential oils (EOs) obtained from the dried aerial parts of four cultivars of Salvia rosmarinus (‘Boule’; ‘Vicomte de Noailles’; ‘Gorizia’; ‘Joyce de Baggio’) and the species S. jordanii, together with their antibacterial and antifungal activities. The phytochemical investigation evidenced a predominance of oxygenated monoterpenes in all the samples (57.5–77.1%), except in ‘Boule’, in which the hydrocarbon form prevailed (50.2%).

  • Rosmarinus officinalis
  • Rosmarinus eriocalyx
  • ‘Boule’
  • ‘Vicomte de Noailles’
  • ‘Gorizia’
  • ‘Joyce de Baggio’
  • hydrodistillation
  • agar diffusion test
  • MIC
  • Principal Component Analysis (PCA)

1. Introduction

The genus Rosmarinus L., belonging to the Lamiaceae family, was recently included in the genus Salvia L. [1]. Salvia subg. rosmarinus comprise three species of aromatic plants [2]: Salvia rosmarinus Spenn. (synonym of Rosmarinus officinalis L., and isonym of Salvia rosmarinus Schleid), Salvia jordanii J.B.Walker (synonym of Rosmarinus eriocalyx Jord. & Fourr.), and Salvia granatensis B.T.Drew (synonym of Rosmarinus tomentosus Hub.-Mor. & Maire) [3,4][3][4]; only the first two species are widely used in traditional medicine [5] and as cooking ingredients [6].
S. rosmarinus, commonly known as “rosemary”, is the most known species. It is an evergreen shrub, able to grow in every type of soil, with predilection for dry and rocky ones. It is no coincidence that it is widespread in the Mediterranean area, in particular in the coastal scrub [7]. It is widely used for its aromatic and medicinal properties [8], determined by its content in secondary metabolites. Among these, the essential oil (EO) responsible for the pleasant smell is the principal product obtained from this plant by industries [9], as it can be exploited for the treatment of skin, digestive, and renal problems, as well as for headaches [10]. On the basis of its main EO components, S. rosmarinus can be distinguished in different major chemotypes, including cineoliferous (1,8-cineole > 40%) [2], camphoriferous (camphor > 20%), verbenoniferoum (verbenone > 15%) [2,11][2][11], and α-pinene chemotype (α-pinene as the major component) [9]. The chemical composition of rosemary essential oil has been widely investigated in relation to different geographical locations of collection, environmental conditions, and seasonal periods. As reported by Cioni et al., soil and climatic conditions only partially modulate the biosynthesis of the main chemicals of the EO which, instead, is mainly determined by the genetic heritage of the plant [12]. Nevertheless, the differences are mainly quantitative rather than qualitative [5].
S. jordanii (ex. R. eriocalyx Jord. & Fourr., previously known as R. tournefortii De Noé), is an aromatic evergreen bush like rosemary, but it has been introduced into cultivation only in the last decades [13]. It is typical of Algeria, Spain and Morocco, preferring mountain rocky grounds and pastures. It differs from S. rosmarinus for some morphological characters such as the smaller leaves, the woolly calyx, and the prostrate growth [6]. As opposite to S. rosmarinus only few studies have been conducted on the chemical composition of S. jordanii essential oil, most of which also investigated its antibiotic activity [5,13,14,15][5][13][14][15].
The essential oils obtained from both S. rosmarinus and S. jordanii have been reported in numerous studies for their antibacterial and antifungal properties, as the aerial part of these plants have been widely used since ancient times as natural preservatives [9,16][9][16]. Abers et al. reported the essential oil of R. officinalis as a good broad-spectrum antibacterial agent [17], and Soulaimani et al. highlighted a better activity against Gram positive bacteria than against the Gram negative ones [18]. In recent times, the EOs, which are complex mixtures of secondary metabolites characterized by high volatility and strong smell [19], have received significant interest for their antibacterial and antifungal properties given the change in consumer behaviour toward a preference for natural products [16,20][16][20].

2. Results and Discussions

2.1. Phytochemical Investigation

The complete compositions and the extraction yields of the essential oils (EOs) obtained from the dried aerial part of the samples are reported in Table 1. The following taxa acronyms were used: Boule = S. rosmarinus ‘Boule’, Gori = S. rosmarinus ‘Gorizia’, Joyce = S. rosmarinus ‘Joyce de Baggio’, Vicom = S. rosmarinus ‘Vicomte de Noailles’, and Jord = S. jordanii. Overall, 65 compounds were identified, accounting for 98.6–100% of the total composition.
Table 1. Complete composition and extraction yield (% w/w dry weight) of the essential oil obtained from the samples of S. rosmarinus and S. jordanii.
Peak Compounds l.r.i. Class. Relative Abundances (%) ±SD
Boule Gori Joyce Vicom Jord


(30 μg/disc)
1 tricyclene 922
S. ser. Typhimurium (S176)
mh - 0.1 ± 0.00
18 (S)
- - 0.1 ± 0.01
19 (S) 15 (R) 21 (S) 25 (S) 21(S)
2 α-thujene 926 mh - 0.2 ± 0.02 0.1 ± 0.00 0.2 ± 0.02 -
Y. enterocolitica (YU3) 22 (S) 27 (S) 17 (I) 0 (R) 32 (S) 22 (S)
3 α-pinene
L. monocytogenes (L1)
933 mh 37.3 ± 3.09 6.4 ± 0.10 25.6 ± 0.02 4.1 ± 0.04 3.1 ± 0.17
26 (S) 0 (R) 28 (S) 21 (S) 10 (R) 22 (S)
4 camphene 948 mh 2.9 ± 0.09
E. durans (EU157) 24 (S) 0 (R) 33 (S)
4.3 ± 0.04 2.0 ± 0.09 3.3 ± 0.10 3.9 ± 0.06
14 (R) 0 (R)
5 thuja-2,4(10)-diene 954 mh 0.3 ± 0.02 - 0.4 ± 0.07 - -
6 β-pinene 977 mh 0.5 ± 0.04 4.2 ± 0.40 1.8 ± 0.07 1.6 ± 0.03 0.9 ± 0.01
7 3-octanone 985 nt - 1.0 ± 0.17 - - -
8 myrcene 991 mh 2.0 ± 0.16 0.6 ± 0.01 0.9 ± 0.09 0.4 ± 0.03 0.2 ± 0.02
9 α-phellandrene 1006 mh - 1.2 ± 0.05 0.2 ± 0.00 - -
10 δ-3-carene 1011 mh - 0.4 ± 0.01 - - -
11 α-terpinene 1017 mh 0.2 ± 0.01 0.5 ± 0.02 0.5 ± 0.01 0.3 ± 0.01 0.7 ± 0.01
12 p-cymene 1025 mh 2.8 ± 0.22 1.0 ± 0.23 0.8 ± 0.08 1.8 ± 0.09 1.6 ± 0.06
13 limonene 1029 mh 3.3 ± 0.11 3.6 ± 0.05 2.1 ± 0.12 1.7 ± 0.14 1.7 ± 0.03
14 1,8-cineole 1031 om 11.4 ± 0.22 20.5 ± 0.73 23.9 ± 0.42 20.0 ± 0.64 11.5 ± 0.11
15 (Z)-β-ocimene 1036 om - - - - 1.2 ± 0.10
16 γ-terpinene 1058 mh 0.4 ± 0.03 1.0 ± 0.10 0.9 ± 0.02 0.6 ± 0.02 0.7 ± 0.02
17 cis-sabinene hydrate 1066 om - 0.3 ± 0.02 - 0.1 ± 0.01 0.1 ± 0.01
18 terpinolene 1089 mh 0.5 ± 0.02 0.6 ± 0.01 0.7 ± 0.03 0.4 ± 0.00 0.2 ± 0.01
19 trans-sabinene hydrate 1098 om - 0.1 ± 0.02 - - -
20 linalool 1101 om 1.5 ± 0.01 0.4 ± 0.04 2.1 ± 0.14 0.2 ± 0.01 -
21 filifolone 1108 om 0.2 ± 0.00 - - - -
22 fenchol 1114 om 0.1 ± 0.01 - - - -
23 cis-p-menth-2-en-1-ol 1122 om - - 0.2 ± 0.03 - -
24 α-campholenal 1125 om - - - 0.2 ± 0.01 -
25 chrysanthenone 1126 om 0.8 ± 0.05 0.2 ± 0.06 0.2 ± 0.01 - -
26 trans-pinocarveol 1139 om 0.2 ± 0.02 0.1 ± 0.02 - - -
27 cis-verbenol 1142 om - 0.1 ± 0.03 0.1 ± 0.03 - -
28 camphor 1145 om 7.7 ± 0.22 16.9 ± 1.35 3.3 ± 0.50 42.2 ± 0.52 33.4 ± 0.38
29 trans-pinocampone 1160 om 0.3 ± 0.01 - 0.2 ± 0.01 0.2 ± 0.02 -
30 pinocarvone 1163 om 0.2 ± 0.00 0.3 ± 0.02 0.3 ± 0.03 0.3 ± 0.07 -
31 borneol 1165 om 2.5 ± 0.11 6.5 ± 0.00 3.7 ± 0.26 0.6 ± 0.13 14.6 ± 0.01
32 isopinocampheol 1173 om 0.4 ± 0.04 - - - -
33 cis-pinocamphone 1174 om - 0.8 ± 0.02 0.8 ± 0.01 0.5 ± 0.00 -
34 4-terpineol 1177 om 1.4 ± 0.08 0.9 ± 0.08 1.1 ± 0.01 1.2 ± 0.02 2.8 ± 0.03
35 p-cymen-8-ol 1185 om 0.1 ± 0.01 - - 0.2 ± 0.01 -
36 α-terpineol 1191 om 2.6 ± 0.19 2.0 ± 0.24 2.4 ± 0.09 2.8 ± 0.07 2.9 ± 0.06
37 myrtenol 1195 om 0.2 ± 0.02 0.2 ± 0.02 0.4 ± 0.14 0.2 ± 0.03 -
38 verbenone 1210 om 12.8 ± 2.67 1.9 ± 0.15 14.9 ± 0.27 2.7 ± 0.03 0.6 ± 0.02
39 trans-carveol 1219 om 0.1 ± 0.06 - - 0.2 ± 0.04 -
40 carvone 1244 om - - - 0.1 ± 0.03 -
41 geraniol 1254 om 0.5 ± 0.08 - 3.9 ± 0.15 - -
42 trans-ascaridol glycol 1268 om - 0.4 ± 0.10 - - -
43 geranial 1271 om - - 0.3 ± 0.02 - -
44 bornyl acetate 1286 om 3.9 ± 0.45 6.5 ± 0.50 2.6 ± 0.17 0.2 ± 0.01 10.8 ± 0.06
45 myrtenyl acetate 1326 om - - 0.1 ± 0.00 - -
46 eugenol 1357 pp - - - - 0.4 ± 0.02
47 α-copaene 1376 sh - 0.3 ± 0.05 - - -
48 geranyl acetate 1385 om - - 0.5 ± 0.03 - -
49 (Z)-jasmone 1397 nt 0.4 ± 0.07 - - - -
50 methyl eugenol 1407 pp - - 0.3 ± 0.02 - -
51 β-caryophyllene 1419 sh 0.2 ± 0.03 6.7 ± 1.51 1.1 ± 0.15 0.6 ± 0.06 3.1 ± 0.08
19 (S)
52 α-humulene 1453 sh - 1.9 ± 0.39 0.3 ± 0.04 - 3.2 ± 0.10
53 γ-muurolene 1477 sh - 0.4 ± 0.08 - - -
54 bicyclogermacrene 1496 sh - 0.3 ± 0.05 - - -
55 trans-γ-cadinene 1514 sh - 0.4 ± 0.07 - - -
56 δ-cadinene 1524 sh - 0.9 ± 0.20 - - -
57 caryophyllene oxide 1582 os 0.4 ± 0.09 4.3 ± 0.16 0.6 ± 0.10 1.5 ± 0.24 0.8 ± 0.02
58 humulene oxide II 1608 os 0.3 ± 0.07 0.5 ± 0.08 - - 0.6 ± 0.03
59 caryophylla-4(14),8(15)-dien-5-ol (unidentified isomer) 1633 os - 0.2 ± 0.06 - 0.3 ± 0.04 -
60 T-cadinol 1641 os - 0.3 ± 0.03 - 0.2 ± 0.03 -
61 α-bisabolol oxide B 1655 os - - - 0.8 ± 0.06 -
62 14-hydroxy-9-epi-(E)-caryophyllene 1670 os - - - 7.1 ± 1.76 0.2 ± 0.01
63 α-bisabolol 1685 os - - - - 0.4 ± 0.01
64 trans-ferruginol 2325 od 0.2 ± 0.04 0.2 ± 0.01 - - -
  Total identified (%)     98.6 ± 0.06 98.7 ± 0.31 99.1 ± 0.16 96.6 ± 0.16 100 ± 0.03
     
E. faecium (EU107) 7 (R) 0 (R) 30 (S)
  Boule Gori Joyce Vicom Jord
0 (R)
  Monoterpene hydrocarbons (mh)   50.2 ± 3.77 A 24.0 ± 0.54 C 36.0 ± 0.19 B 14.3 ± 0.46 D 14.2 ± 0.49 D
  Oxygenated monoterpenes (om)   46.9 ± 3.42 C 57.5 ± 2.08 B 60.9 ± 0.04 B 71.9 ± 1.55 A 77.1 ± 0.25 A
0 (R)
  Sesquiterpene hydrocarbons (sh)   0.2 ± 0.03 C 10.7 ± 2.35 A 1.3 ± 0.19 C 0.6 ± 0.06 C 6.3 ± 0.18 B
18 (S)
  Oxygenated sesquiterpenes (os)   0.7 ± 0.16 C 5.3 ± 0.33 B 0.6 ± 0.10 C 9.8 ± 2.12 A 2.0 ± 0.07 C
  Oxygenates diterpenes (od)   0.2 ± 0.04 A 0.2 ± 0.01 A - B - B - B
  Phenylpropanoids (pp)   - - 0.3 ± 0.02 - 0.4 ± 0.02
  Other non-terpene derivates (nt)   0.4 ± 0.07 B 1.0 ± 0.17 A - C - C - C
  EO Extraction yield (%w/w)   0.57 ± 0.02 C 1.17 ± 0.16 B 0.76 ± 0.04 C 2.25 ± 0.15 A 0.71 ± 0.04 C
According to Flamini et al. (2020) [2] monoterpenes are the main class of compounds in Rosmarinus genus: indeed, the EO obtained from S. rosmarinus ‘Boule’ was characterized by a predominance of their hydrocarbon derivatives (50.2%), while those obtained from the other four samples presented more oxygenated ones.
Nevertheless, monoterpene hydrocarbons were well-represented in all the samples, accounting for up to 50.2% in Boule, followed by Joyce (36.0%), Gori (24.0%), Jord, and Vicom (14.3 and 14.2%, respectively). α-Pinene, camphene, β-pinene, and limonene were the main chemicals of this class, but only the first reached considerable relative amounts, up to 37.3% in ‘Boule’ and 25.6% in ‘Joyce’.
Oxygenated monoterpenes, indeed, resulted more abundant in Jord and Vicom (77.1% and 71.9%, respectively), followed by Joyce and Gori (60.9% and 57.5%, respectively) and Boule (46.9%). Within this chemical class, 1,8-cineole (11.4–23.9%), camphor (3.3–42.2%), borneol (0.6–14.6%), 4-terpineol 0.9–2.8%), α-terpineol (2.0–2.9%), verbenone (0.6–14.9%), and bornyl acetate (0.2–10.8%) were the most representative compounds, as they were detected in all the samples, even though with a high variability in their relative abundances.
Sesquiterpenes were also detected in appreciable relative amounts: the EOs obtained from Gori and Jord presented a predominance of the hydrocarbons form, and Vicom of the oxygenated form. In Boule and Joyce, this chemical class was poorly represented. β-Caryophyllene (0.2–6.7%) and caryophyllene oxide (0.4–4.3%) were identified in each sample; noteworthy was the amount of 14-hydroxy-9-epi-(E)-caryophyllene in Vicom (7.1%).
All the most representative compounds detected in the EOs were typical chemicals of the essential oils of S. rosmarinus [9] and S. jordanii [5,14][5][14].
The EO extraction yield presented significant differences among the samples: Vicom was the most productive one (2.25% w/w), followed by Gori (1.17% w/w), while Joyce, Jord, and Boule presented the lowest yields (0.76 > 0.71 > 0.56% w/w, respectively).
This is the first time that the essential oil composition of the four cultivars of S. rosmarinus (‘Boule’; ‘Vicomte de Noailles’; ‘Gorizia’; ‘Joyce de Baggio’) was reported to better employ this plant material not only as ornamental display items but also as derivative products for industrial use.

Statistical Analysis

The first axis of PCA explained 62.4% of variance, the second axis (PCA2) a further 25.1% (Figure 1). Vicom segregated alone, while the other taxa were distributed into two groups, one formed by Gori and Jord, and the other one made up by Boule and Joyce. Nine chemical compounds showed a significant discriminative function between the taxa. Vicom was characterized by high amounts of camphor (28) and 14-hydroxy-9-epi-(E)-caryophyllene (62). Gori and Jord differed in their predominance of camphene (4), borneol (31), bornyl acetate (44), and α-humulene (52). Lastly, Boule and Joyce were characterized by high amounts of α-pinene (3), myrcene (8), and verbenone (38).
Antibiotics 10 01090 g001 550
Figure 1. PCA of the matrix 5 taxa × 18 compounds. Compounds with a Pearson correlation coefficient > 0.8 with the first two PCA axes are shown. Abbreviations of chemical compounds: 3 = α-pinene, 4 = camphene, 8 = myrcene, 28 = camphor, 31 = borneol, 38 = verbenone, 44 = bornyl acetate, 52 = α-humulene, 62 = 14-hydroxy-9-epi-(E)-caryophyllene.

2.2. Antimicrobial Investigation

In vitro antibiotic sensitivity tests detected multi-resistance of the assayed bacterial isolates (Table 2).
Table 2. The inhibition zones expressed in millimeters resulted from the application of different antibiotics against the selected bacterial strains (S: susceptible; R: resistant; I: intermediate).
Antibiotics  
STRAINS Tetracycline

(30 μg/disc)		 Ceftazidime

(30 μg/disc)		 Rifampicin

(30 μg/disc)		 Cephalexin

(30 μg/disc)		 Cefotaxime

(30 μg/disc)		 Chloramphenicol
E. faecalis
(EU37)
10 (R) 0 (R) 15 (R) 13 (R) 18 (I) 19 (S)
Enterococci emerged as the bacterial isolate with resistance to most antibiotics. Enterococcus spp. have a strong ability to acquire, express and transfer antimicrobial resistance [21], and our findings are in agreement with other studies reported in the literature [22].

43. Conclusions

All the analysed EOs were characterized by a predominance of oxygenated monoterpenes (57.5–77.1%), except that of ‘Boule’, in which the hydrocarbon form prevailed (50.2%). Considering the whole chemical composition, a total of nine compounds showed a significant discriminative function between the samples. ‘Vicomte de Noailles’ was characterized by high amounts of camphor and 14-hydroxy-9-epi-(E)-caryophyllene; ‘Gorizia’ and Jord differed for their predominance of camphene, borneol, bornyl acetate, and α-humulene and, lastly, ‘Boule’ and ‘Joyce de Baggio’ were characterized by high amounts of α-pinene, myrcene, and verbenone.
 

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