HDAC6: Comparison
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Histone deacetylase (HDAC) 6 is a zinc-dependent enzyme of HDAC class IIb. HDAC6 is unique within the HDAC family due to a particular structure giving it unique biological functions implicated in all major cell pathways. This isoenzyme is mainly active in the cytoplasm and possesses two functional catalytic sites and an ubiquitin-binding domain. The deacetylase functions of HDAC6 targets multiple substrates including essentially α-tubulin and heat shock protein (HSP)90α which are key factors in cell regulatory networks through the regulation of the microtubule network and many protein functions, respectively. Accordingly, several studies have highlighted the role of HDAC6 in various pathological conditions. For instance HDAC6 overexpression frequently correlates with tumorigenesis and favor cell survival and metastasis. Therefore, HDAC6 represents an interesting potential therapeutic target.

  • histone deacetylase 6 inhibitor
  • personalized treatment
  • heat shock protein 90α
  • leukemia stem cells
  • imatinib resistance
  • targeted therapy

1. Introduction

Carcinogenesis is a multistep process whereby normal cells are transformed into malignant cells. The process is characterized by major biological changes shared by most neoplastic cells called hallmarks of cancer. These transformational events relies on multiple alterations at genetic and epigenetic levels leading to abnormal cell growth [1].

Over the past years protein lysine acetylation has emerged as a key post-translational modification in the coordination of tightly regulated biological functions and alterations of the acetylome profiles are associated with various pathological conditions such as cancer.

The acetylation status of lysine residues within histone and non-histone proteins is finely tuned by the concert action of histone acetyltransferases (HATs) and histone deacetylases (HDACs) catalyzing the addition and removal of the acetyl groups, respectively. Recently, there was a particular focus on HDAC6 coming from its unique properties to control multiple cellular pathways linked to cell growth, survival, and migration. Accordingly, the use of HDAC6 inhibitors alone or in combination with additional chemotherapeutic agents appear as a promising strategy to treat various cancers.

2. Histone Deacetylase 6

The HDAC6 protein is part of the HDAC family, which are enzymes catalyzing the deacetylation of proteins, which corresponds to the removal of an acetyl group from lysine residues [2]. The 18 HDACs found in mammals are divided into four classes according to their sequence homology. For classes I (HDAC1, 2, 3, and 8), IIa (HDAC4, 5, 7, and 9), IIb (HDAC6 and 10), and IV (HDAC11), the deacetylation of lysine occurs through a transfer of charge, and their essential component is a zinc ion (Zn2+) present at the bottom of the catalytic pocket of HDAC enzymes [3]. For class III [sirtuins (SIRT) 1-7] HDACs, the presence of a cofactor, nicotinamide adenine dinucleotide (NAD+), is essential for the reaction [4][5].
Class I HDACs are ubiquitously present in many human cell lines and tissues, while class II HDACs exhibit a specific expression profile in certain human tissues such as the heart (HDAC5), the breast (HDAC6), the ovary (HDAC7 and 9), and the kidney (HDAC10) [6][7].

2.1. Structure

Here, we will focus more specifically on HDAC6 belonging to class IIb. This enzyme is the only HDAC to possess two functional active catalytic sites, and has a nuclear localization sequence, a nuclear export sequence, and a repetitive region of eight consecutive serine-glutamic acid tetradecapeptides, a cytoplasmic retention signal, and is mainly present in the cytoplasm [8]. HDAC6 also has a C-terminal ubiquitin-binding domain required when binding to poly-ubiquitinated proteins (Figure 1).
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Figure 1. Protein structure of histone deacetylase 6 (HDAC6). The HDAC6 protein consists of 1215 amino acids. It has a nuclear localization sequence (NLS), a nuclear export sequence (NES), two functional active catalytic (SC) sites, a cytoplasmic retention signal of eight consecutive serine-glutamic acid tetradecapeptides (SE14), and a ubiquitin-binding domain (BUZ) at the C-terminal.

2.2. Function

The HDAC6 protein deacetylates many substrates [9] (Table 1) including α-tubulin, cortactin, and heat shock protein (HSP)90α, and is thus involved in many cell processes, some of which are described below [10].
Table 1. List of substrates specifically deacetylated by HDAC6.
Substrates Localization of the Substrate Deacetylated Lysine(s) Function of the Deacetylated Substrate Interaction Domains of HDAC6 Reference
14-3-3ζ Cytoplasm and nucleus 49, 120 Regulation of protein binding Bad and AS160 ND [11]
In addition to these known post-translational modifications, there are proteins interacting directly with the HDAC6 protein and inducing its inhibition by direct interaction (Table 3).
Table 3. Proteins that interact directly with the HDAC6 protein.
Protein Inhibiting HDAC6 by Direct Interaction Protein Function Protein Region Required for Interaction with HDAC6 HDAC6 Domain Interacting with the Protein Cellular Impact References
CYLD Deubiquitinase ND DD1/DD2 Cell proliferation, ciliogenesis [10]
β-catenin Cytoplasm and nucleus 49 Epidermal growth factor-induced nuclear localization and decreased expression of c-Myc ND [9]
Dysferlin Skeletal muscle membrane repair, myogenesis, cell adhesion, intercellular calcium signaling Domain C2 ND Myogenesis [34] Cortactin * Cytoplasm 87, 124, 161, 189, 198, 235, 272, 309, 319 Regulation of cell migration and actin filament binding DD1 and DD2 ND[9]
Increased deacetylation activity of α-tubulin [32]
Aurora ND Increased deacetylation activity of α-tubulin [10]
Mdp3 Stabilization factor of microtubules DNAJA1 Cytoplasm ND
DD: deacetylase domain; Mdp3: microtubule-associated protein (MAP) 7 domain-containing protein 3; ND: non determined; RanBPM: Ran-binding protein microtubule-organizing center; tau: tubulin-associated unit; TPPP1: tubulin polymerization-promoting protein-1.

3. HDAC6 in cancer

Several studies have demonstrated the influence of HDAC6 in neurodegenerative, cardiovascular and renal diseases, as well as in inflammation [40] and viral response [10]. The role of the HDAC6 protein in cancer is also now well better understood. Although its oncogenic or tumor suppressor potential is dependent on the type of cancer [28], its involvement in oncogenic cell transformation, tumor development, and cancer immunity regulation makes a strong therapeutic candidate [41].

HDAC6 is overexpressed in many types of cancer (Table 4) and may be implicated in disease progression.
Table 4. Deregulation of HDAC6 expression in different types of cancers.
Cancer Type Cancers Expression of HDAC6-Comments References
Solid tumors Bladder Overexpressed [41]
Melanoma Overexpressed [41]
Peripheral T-cell lymphoma
Overexpressed
[
46
]
CD: cluster of differentiation; HDAC6: histone deacetylase 6.

3.1. HDAC6 Inhibitors

The ability to specifically target HDAC6 would have valuable clinical utility in the treatment of these cancers. However, despite a large number of pan-HDAC inhibitors, very few compounds are capable of selectively inhibiting HDAC6 (Table 5). This type of inhibitor can be divided into 2 groups according to their chemical structure: benzamides and hydroxamates [28].
Table 5. List of HDAC6 inhibitors.
Class HDAC6 Inhibitor Binding Domain CI50 (nM) of the HDAC6 Activity in Vitro Selectivity Ratio for HDAC6 Compared to (Other HDACs) Inhibition of HDAC6 in Cellulo (µM)$ Effect on Cancer Cell Lines or Cancer Type References
Benzamides Trithiocarbonate derivative (12ac) ND 65 19 (HDAC1) 10 (lung cancer) CI50 = 8.2 µM (cervical cancer) [47]
  NQN-1 (2-benzyl-amino-naphthoquinone) ND 5540 Values non available (HDAC1, 2, 3, 4, 5, 7, 8, 9, 10, 11) 4 (chronic myeloid leukemia) CI50 = 0.8 µM (leukemia) [48]
Amino-terminal region ND Cell motility [
Lung Overexpressed41]35 [] Protein folding ND [12]
Region rich in proline ND Polarization and cell migration [10] ERK1 Cytoplasm and nucleus 72 Proliferation, mobility, and cell survival ND PKCζ[ ND Increased deacetylation activity of α-tubulin [10]
p62 Transport of misfolded proteins Between the ZZ domain and the TRAF6 link area DD2 Aggresome formation
Hydroxamates Hydroxamic acid containing a phenylalanine (4n) His215, His216, Tyr386, Phe283, and Tyr255 of DD1 and His610, His611, Tyr782, Phe620, and Phe680 of an HDAC6 homology model 1690 14 (HDAC1) 1 (colorectal carcinoma) IC50: 3 to > 50 µM (various cancer cell lines) [49] Oral squamous cell carcinoma Overexpressed-Enhanced expression in advanced stages [28][ 55 (HDAC1), 75 (HDAC2), 128 (HDAC3) 2.5 (neural cells)
CKD-506
ND
5
>400 (HDAC1, 2, 7, 8)
0.03 (Human PBMCs)
ND
[
100
]
Arg: arginine; Asp: aspartic acid; CI50: concentration inhibiting 50% of cell viability; DD: deacetylase domain; Glu: glutamic acid; HDAC: histone deacetylase; His: histidine; IC50: concentration inhibiting 50% of cell growth; Leu: leucine; ND: non determined; PBMC: peripheral blood mononuclear cell; Phe: phenylalanine; Pro: proline; SAHA: suberoylanilide hydroxamic acid; Ser: serine; Tyr: tyrosine.
The compounds ACY-241 (Citarinostat) and ACY-1215 (Ricolinostat) are derivatives of hydroxamic acid, which shows a specific inhibitory activity against HDAC6 with IC50 values of 2.6 and 5 nM, respectively. They are the only HDAC6 inhibitors in currently clinical trials (Table 6) [58][88]. To date, no HDAC6 inhibitor has yet been approved by the FDA, unlike pan HDAC inhibitors such as  romidepsin, suberoylanilide hydroxamic acid (SAHA, vorinostat), PXD101 (belinostat), and LBH589 (panobinostat) [101].
Table 6. HDAC6 Inhibitors in Clinical Trials in Cancer. Clinical studies include four phases. Phase I is performed on healthy volunteers to determine the maximum tolerated dose in humans. Phase II is performed on a limited patient population to determine the optimal dosage. Phase III is performed on several thousand patients and will demonstrate the therapeutic value of the drug and assess its benefit/risk. Phase IV is performed once the drug is marketed and allows to better characterize its adverse effects.
HDAC6 Inhibitor Clinical Trial Identification Phase of the Clinical Trial Pathology
ACY-241 NCT02400242 Ia/Ib Multiple myeloma
  NCT02935790 Ib Stage III and IV unresectable melanoma
  NCT02551185 Ib Advanced solid tumors
  NCT0263506142] Ib
13]
[ Foxp3 * Nucleus ND ND ND [14]
36]
  Hydroxamic acid containing a pyridylalanine (5a) Phe566 of DD2 of an HDAC6 homology model 3970 25 (HDAC1) ND IC50: 104 µM (breast cancer) Non-resectable non-small cell lung cancer[50 ND
ACY-1215 NCT02632071[ Ib51] CK2 Ser-458
Unresectable or metastatic breast cancer Improved formation and elimination of aggresomes [10]   HDAC9 Cytoplasm and nucleus ND Modulation of cell survival and arrest of cellular movement DD2 [15]
ACY-775 HDAC11 Nucleus ND Transcriptional activation of interleukin 10 ND [16]
HMGN2 Nucleus 2 Increased transcription of STAT5 ND [17]
Paxillin Focal adhesion
] Ovarian carcinoma Overexpressed-Enhanced expression in advanced stages [28][42] RanBPM Apoptosis, proliferation and cell migration   ND Aggresome formation [37] EGFR Tyr-570 Inhibition of deacetylation activity [33]
Breast Overexpressed-Prediction of a good or bad prognosis [28][43] ND 7.5 283 (HDAC1), 343 (HDAC2), 1496 (HDAC3) 2.5 (neural cells) ND [51] Tau Stabilization factor of microtubules Tubulin binding region SE14 domain Aggresome formation [36][38] Acetylation p300
Hepatocytic carcinoma
  NCT02787369 Ib Relapsed chronic lymphocytic leukemia Overexpressed-Enhanced expression in advanced stages [28]   ACY-1083 His573 and His574 of DD2 3 260 (HDAC1) 0.03 (neuroblastoma) ND [52][53] TPPP1
  NCT02091063 Ib/II Relapsed or refractory lymphoid malignancies Polymerization and acetylation of microtubulesLys-16 Inhibition of deacetylation activity [  10   Bavarostat
  NCT01997840Ser568 of DD2 60 >10000 (HDAC1, 2, 3), 188 (HDAC4), 317 (HDAC5), 78 (HDAC7), 142 (HDAC8), 87 (HDAC9), >17 (HDAC10), 167 (HDAC11) 10 (neural progenitor cells derived from induced pluripotent stem cells) ND [54 HSC70 Cytoplasm ND Protein folding ND [12]
] HSPA5 Cytoplasm 353 Ubiquitination of HSPA5 mediated by GP78 ND [18]
HSP90α Cytoplasm 294 Degradation and elimination of misfolded proteins and regulation of glucocorticoid receptors DD1, DD2 et BUZ [9]
ND]
CK2: casein kinase 2; EGFR: epidermal growth factor receptor; ERK1: extracellular signal-regulated kinase; GRK2: G protein-coupled receptor kinase 2; GSK3: glycogen synthase kinase 3; Lys: lysine; ND: non determined; PKCζ: protein kinase C isoform ζ; Ser: serine; Thr: threonine.
Under-expressed-HDAC6 suggested as a tumor suppressor
[
28
]
[
44
]
Ib/II Recurrent and refractory multiple myeloma Hematological Chronic lymphocytic leukemia Overexpressed-Observation on patient samples, cell lines and a transgenic mouse model [  42]
BRD9757
  NCT01583283ND 30 21 (HDAC1), 60 (HDAC2), 23 (HDAC3), 727 (HDAC4), 611 (HDAC5), 420 (HDAC7), 36 (HDAC8), >1000 (HDAC9) I/II Multiple myeloma recurrent or recurrent and refractory10 (cervical cancer) ND Acute myeloid leukemia Overexpressed [28][42]
  ACY-738 ND 1.7 Regulation of microtubule acetylation and β-catenin expression [39]
[
55
]
 
Cay10603
His499 of DD2 of an HDAC6 homology model 0.002 ND <1 to 1 µM (several pancreatic cancer cell lines) ND [56 Ib][57] Recurrent and refractory multiple myeloma
Acute lymphoblastic leukemia
 Overexpressed-Enhanced expression in advanced stages Citarinostat (ACY-241)[28] ND 2.6
 
14 (HDAC1), 17 (HDAC2), 18 (HDAC3 and 4), >7000 (HDAC4, 5,9), 2808 (HDAC7), 53 (HDAC8), 0.3 (ovarian cancer) CI50: 4.6 to 6.1 µM (ovarian and breast cancer) [58] Chronic lymphocytic leukemia Overexpressed-Correlated with longer survival [
 
  NCT01323751 I/II Multiple myeloma recurrent or recurrent and refractory α3β-cyclic tetrapeptide (23)28 ND 39 3 (HDAC1), 4 (HDAC3), 6 (HDAC8) 2 (acute lymphoblastic leukemia) IC50: 9 to > 20 µM (various cancer cell lines)
  NCT02856568[ Ib59 Unresectable or metastatic cholangiocarcinoma K-RAS * Cytoplasm 104 Cell proliferation ND [19]
]
] T-cell cutaneous lymphoma Overexpressed-Correlated with longer survival
  NCT02661815[28] Ib Ku70 Cytoplasm
Ovarian cancer, primary peritoneal cancer or platinum-resistant fallopian tubes Chronic myeloid leukemia539, 542 Suppression of apoptosis ND Overexpressed-Increased expression in CD34+ cells [45][9]
LC3B-II* Cytoplasm
Multiple myeloma OverexpressedND Regulation of autophagy ND [46][
NCT02189343   Compound containing a phenylisoxazole group as a surface recognition group (7) His499 of HDAC7 0.002 >100000 (HDAC1), >100000 (HDAC2), 210 (HDAC3), >3000000 (HDAC8), 45350 (HDAC10) ND IC50: 0.1 to 1 µM (various prostate cancer cell lines) [20]
56] MSH2 Cytoplasm and nucleus 845, 847, 871, 892 Reduced cellular sensitivity to DNA damaging agents and reduced DNA mismatch repair activities by downregulation of MSH2
  Compound containing a triazolylphenyl group (6b) ND 1.9 52 (HDAC1), 155 (HDAC2), 7 (HDAC3), 420 (HDAC8), 59 (HDAC10) ND IC50: <0.5 to 22 µM (several prostate cancer lines) [60]
  Compound containing a peptoid (2i)Mantle cell lymphoma OverexpressedDD1 [46[21]
Tyr301 of DD2 of an HDAC6 homology model 1.59 126 (HDAC2), >6000 (HDAC4), 40 (HDAC11) N IC50: 0.34 to 2.7 µM (various cancer cell lines) [61] ]
  3-aminopyrrolidinone derivative (33) ND 17 4359 (HDAC1), 11 (HDAC8) 0.3 (multiple myeloma) Good oral bioavailability [62] MYH9 Cytoplasm ND Regulation of binding to actin filaments ND [12
Diffuse large B cell lymphoma Overexpressed]
[
 46] 4-aminomethylaryl acid derivative (1a) ND 19 305 (HDAC1), 842 (HDAC2), 237 (HDAC3), 790 (HDAC4), 174 (HDAC5), 242 (HDAC7), 36 (HDAC8), 195 (HDAC0) 0.46 (cervical cancer) ND [63] PrxI Cytoplasm and nucleus 197 Antioxidant activity ND [22][23]
  4-hydroxybenzoic acid derivative (7b) ND 200 >50000 (HDAC1, 2, 8), >500000 (HDAC3, 10, 11) 50 (prostate cancer) IC50: 41 to 130 (several prostate and breast cancer cell lines) [64] PrxII Cytoplasm and nucleus 196 Antioxidant activity ND [22][23]
RIG-I Cytoplasm 858, 909 Recognition of viral RNA ND [24]
  4-hydroxybenzoic acid derivative (13a) ND 20000 25 (HDAC1), >5000 (HDAC2, 3, 4, 8, 10), >2500 (HDAC11) 50 (prostate cancer) IC50: 19 to 127 (several prostate and breast cancer cell lines) [64]
  Aminoteraline derivative (32) Phe620 and Phe680 of an HDAC6 homology model 50 126 (HDAC1), 2 (HDAC8) 2 (neuroblastoma) IC50 = 5.4 µM (neuroblastoma) [65] Sam68 Nucleus ND Alternative splicing ND [
 25]
Benzothiophene derivative (39) ND 14 ND Same effect as tubastatin A Does not target NF-κB and AP-1 at the transcriptional level [66] Survivin Nucleus 129 Anti-apoptotic function DD2 [9]
Tat Cytoplasm 28 Suppression of HIV transactivation DD2 and BUZ [26]
α-tubulin * Cytoplasm 40 Formation of immune synapses, viral infection, cell migration and chemotaxis DD1 or DD2 [9][27]
* Cortactin and LC3B-II are also deacetylated by SIRT1, K-RAS and α-tubulin are also deacetylated by SIRT2, and Foxp3 is also deacetylated by HDAC9 and SIRT1. AS160: Akt substrate of 160 kDa; Bad: Bcl-2 associated agonist of cell death; BUZ: binding-of-ubiquitin zinc; DD: deacetylase domain; DNAJA1: dnaJ homolog subfamily A member 1; ERK1: extracellular signal-regulated kinase 1; Foxp3: forkhead box P3; GP: glycoprotein; HDAC: histone deacetylase; HIV: human immunodeficiency virus; HMGN2: high mobility group nucleosomal binding domain 2; HSC: heat shock cognate; HSP (A): heat shock protein [family A (HSP70) member 5]; LC3B-II: microtubule-associated protein 1 light chain 3; MSH2: MutS protein homolog 2; MYH9: myosin heavy chain 9; ND: non determined; Prx: peroxiredoxin; RIG-I: retinoic acid-inducible gene I protein; Sam: Src-associated substrate in mitosis; STAT: signal transducer and transcriptional activator; Tat: twin-arginine translocation protein.
The HDAC6 protein plays an important role in the dynamism of two components of the cytoskeleton, actin filaments (or F-actin) and microtubules, α- and β-tubulin polymers, which are involved in particular in cell mobility and division. Cortactin, which improves the polymerization of actin filaments, and α-tubulin, a constituent of microtubules, are substrates of HDAC6. The deacetylation of cortactin by HDAC6 and SIRT1 leads to its binding to F-actin, improving its polymerization, and thus contributing to cytoskeletal dynamics (Figure 2A). The deacetylation of α-tubulin by HDAC6 and SIRT2 is associated with microtubule depolymerization, thus contributing to the dynamism of microtubules (Figure 2B), and to proteasome-independent protein degradation. When the proteasome is degraded, the polyubiquitinated misfolded proteins are transported to the microtubule-organizing center and are supported by HDAC6 via its ubiquitin binding domain, leading to the formation of aggresomes through deacetylation of cortactin. The aggresomes thus formed are subsequently removed after autophagosome fusion with lysosomes via autophagy (Figure 2C). A decrease in the acetylation of microtubule-associated protein 1 light chain 3 by HDAC6 was observed during autophagic degradation [28][29]. The HDAC6 protein is also involved in proteasome-dependent protein degradation via its interaction with HSP90α, a chaperone that stabilizes other proteins when deacetylated by HDAC6. In its acetylated form, HSP90α loses its chaperone function, which leads to the degradation of its client proteins by the proteasome (Figure 2D). An accumulation of misfolded proteins causes dissociation of the complex containing HSP90α, heat shock factor 1 (HSF) 1, chaperone valosin-containing protein/ATPase, and HDAC6. In complex in inactive form, during dissociation, the release of HSF1 induces the transcription of many HSPs (Figure 2E), and HDAC6 will allow its binding to misfolded proteins [28].
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Figure 2. The HDAC6 protein is involved in many cellular processes. HDAC6 is involved in F-actin polymerization (A), microtubule dynamics (B), anti-apoptotic activity (C), proteasome-dependent and -independent degradation (D), transcriptional activation of chaperone proteins (E), and autophagy (F). Ac: acetylated; HAT: histone acetyltransferase; HDAC: histone deacetylase; HSF: heat shock factor; HSP: heat shock protein; MTOC: microtubule organizing center; SIRT: sirtuin; VCP: valosin-containing protein/ATPase.
HDAC6 is also involved in apoptosis by deacetylating the Ku70 protein, which then forms a complex with BAX, a proapoptotic protein, allowing the inhibition of apoptosis (Figure 2F). Similarly, inhibition of the catalytic activity of HDAC6 promotes the dephosphorylation of AKT and ERK, associated with decreased cell proliferation and death of cancer cells [28].
Furthermore, HDAC6 regulates endocytosis and exocytosis vesicles. When the epidermal growth factor receptor (EGFR) receptor is bound to its ligand, it interacts with HDAC6 and inactivates it by phosphorylation, which then leads to the hyperacetylation of microtubules and finally the internalization of the receptor. The inhibition of HDAC6 induces the increase of the acetylation of peroxiredoxins 1 and 2, which are antioxidant enzymes, increasing their activity and causing a reduction in cell resistance to chemotherapy [28]. HDAC6 is involved in the process of autophosphorylation of tau protein, giving it the ability to form aggregates called neurofibrillary tangles that can cause neurotoxicity [30].

2.3. Post-Transcriptional Regulation

There is a lack of current data explaining the post-transcriptional regulation of HDAC6 protein. Nevertheless, some microRNAs stimulating cancer cell proliferation and metastasis formation (miR-22, miR-221, miR-433, and miR-548) [10], and stem cell differentiation (miR-26a) [31], are predicted to interact with HDAC6 protein, thus inducing a destabilization or repression of the translation of its mRNA.

2.4. Post-Translational Regulation

Post-translational modifications such as phosphorylation and acetylation have a significant impact on HDAC6 functions. Indeed, although EGFR induces an inhibitory phosphorylation of HDAC6, in the majority of cases it is established that the phosphorylation of HDAC6 improves its deacetylase activity, whereas acetylation decreases its enzymatic activity, preventing the deacetylation of α-tubulin. Examples of post-translational modifications of the HDAC6 protein influencing its activity are shown in Table 2.
Table 2. Post-translational modifications regulating the activity of HDAC6.
Post-Translational Modification Enzyme Target Site Consequences Reference
Phosphorylation GSK3β Ser-22 Increased deacetylation activity of α-tubulin [10]
ERK1 Ser-1035 Regulation of cellular motility [10]
GRK2
 
2,4-imidazolinedione derivative (10c)
ND
4.4
218 (HDAC1), 63 (HDAC2), 53 (HDAC3), > 20000 (HDAC4, 7, 8, 9, 11), 3386 (HDAC5), 37 (HDAC10)
1.6 (acute myeloid leukemia)
IC
50
: 0.2 to 0.8 µM (various cancer cell lines)
[67]
  Mercaptoacetamide derivative (2) ND 95.3 34 (HDAC1), 77 (HDAC2), 64 (HDAC8), 112 (HDAC10) ND At 10 µM protects cortical neurons from oxidative stress inducing death [68]
  N-Hydroxycarbonylbenylamino quinoline derivative (13) ND 0.291 32817 (HDAC1), 42955 (HDAC2), 26632 (HDAC3), 15250 (HDAC4), 10694 (HDAC5), 2436 (HDAC7), 4089 (HDAC8), 5258 (HDAC9), 33646 (HDAC10), 1292 (HDAC11) 0.1 (multiple myeloma) IC50: 9.1 to 40.6 µM (multiple myeloma) [69]
  Isoxazole-3-hydroxamate derivative (SS-208) His463, Pro464, Phe583, and Leu712 of DD2 12 116 (HDAC1), 1625 (HDAC4), 576 (HDAC5), 695 (HDAC7), 103 (HDAC8), 3183 (HDAC9), 427 (HDAC11) 5 (melanoma) ND [70]
  Phenothiazine derivative (7i) Phe620 and Phe680 of DD2 5 538 (HDAC1) 0.1 (acute myeloid leukemia) ND [71]
  Phenylhydroxamate derivative (2) Phe464 and His614 of DD2 3 27 (HDAC1) ND CI50: 0.65 to 2.77 (ovarian cancer and squamous cell carcinoma of the tongue) [61][72]
  Phenylsulfonylfuroxan derivative (5c) ND 7.4 33 (HDAC1), 51 (HDAC2), 45 (HDAC3), 4 (HDAC4), 46 (HDAC8), 82 (HDAC11) 0.013 (acute myeloid leukemia) IC50: 0.4 to 5.8 µM (various cancer cell lines) [73]
  Pyridone derivative (11e) Phe155 and Phe210 of HDAC2 2.46 8 (HDAC1), 52 (HDAC2), 127 (HDAC3), 2329 (HDAC4), 785 (HDAC5), 1512 (HDAC7), 77 (HDAC8), 2268 (HDAC9), 21 (HDAC10), 22 (HDAC11) ND IC50: 0.14 to 0.38 µM (various cancer cell lines) [74]
  Pyrimidinedione derivative (6) ND 12.4 138 (HDAC1), 444 (HDAC2) ND Induces arrest of the cell cycle in subG1 phase and death by apoptosis (colon cancer) [66][75]
  Quinazolin-4-one derivative (3f) ND 29 65 (HDAC1), 222 (HDAC2), 60 (HDAC18), 141 (HDAC11) Increases acetylation levels of α-tubulin and histone H3 at 10 μM ND [76]
  Sulfone derivative (36) ND 8 138 (HDAC8), 300 (HDAC11) 0.01 (unspecified) ND [66]
  Trichostatine A derivatives (M344, 16b) ND 88 3 (HDAC1) ND ND [77]
  Tubacin derivative (WT-161) Phe200, Phe201, Leu270, Arg194 of HDAC7 0.4 129 (HDAC3) 0.3 (multiple myeloma) IC50 = 3.6 µM (multiple myeloma)SangtingTaoCI50: 1.5 to 4.7 µM (multiple myeloma cell lines) [78]
  Tubastatin A derivative (Marbostat-100) Asp649, His651 et Asp742 of DD2 0.7 1106 (HDAC2), 247 (HDAC8) 0.05 (acute monocytic leukemia) Non-cytotoxic [79]
  Indolylsulfonylcinnamic hydroxamate (12) ND 5.2 60 (HDAC1), 223 (HDAC2) 0.1 (colon cancer) IC50: 0.4 to 2.5 µM (multiple cancer cell lines) [80]
  MAIP-032 DD2 58 38 (HDAC1) ND CI50: 3.87 µM (squamous cell carcinoma line of the tongue) [81]
  MPT0G211 ND 0.291 ND 0.1 (neuroblastoma) ND [30]
  N-hydroxy-4-[(N(2-hydroxyethyl)-2-phenylacetamido)methyl)-benzamide)] (HPB) His573 and His574 of DD2 31 37 (HDAC1) 8 (prostate cancer) ND [52][82]
  N-hydroxy-4-(2-[(2-hydroxyethyl)(phenyl)amino]-2-oxoethyl)benzamide (HPOB) Binding to zinc ion only via its OH group but does not displace the zinc-bound water molecule 56 52 (HDAC1) 16 (prostate cancer, adenocarcinoma, glioblastoma) Increases the effect on cell viability in combination with etoposide, dexamethasone or SAHA [83][84]
  N-hydroxy-4-(2-methoxy-5-(methyl(2-methylquinazolin-4-yl)-amino)phenoxy)butanamide (23bb) Tyr298 and Glu255 of an HDAC6 homology model 17 25 (HDAC1), 200 (HDAC8) 0.051 (cervical cancer) IC50: 14 to 104 nM (various cancer cell lines) [85]
  Nexturastat A DD2 of an HDAC6 homology model 5 604 (HDAC1) 0.01 (murine melanoma) IC50 = 14.3 µM (melanoma) [57][86]
  Oxazole hydroxamate (4g) Phe620, Phe680, Leu749, and Tyr782 of DD2 of an HDAC6 homology model 59 237 (HDAC1, 8) 10 (cervical cancer) IC50 = 10.2 µM (acute myeloid leukemia) [87]
  Ricolinostat (ACY-1215) DD2 of an HDAC6 homology model 4.7 12 (HDAC1), 10 (HDAC2), 11 (HDAC3), 1490 (HDAC4), 1064 (HDAC5), 298 (HDAC7), 21 (HDAC8), >2000 (HDAC9, 11) 0.62 (multiple myeloma) CI50: 2 to 8 µM (multiple myeloma cell lines) [57][88][89]
  Sahaquine ND ND ND 0.1 (glioblastoma) CI50: 10 µM (glioblastoma) [90]
  TC24 Ser568, His610, Phe679 and Tyr782 of HDAC6 ND ND 1 et 10 (gastric cancer) CI50: 10.2 to 17.2 µM (several gastric cancer cell lines) [91]
  Tetrahydroisoquinoline (5a) ND 36 1250 (HDAC1), >1000 (HDAC2, 4, 5, 7, 10, 11), 1278 (HDAC3), 58 (HDAC8) 0.21 (cervical cancer) ND [63]
  Thiazole ND 52 ND ND ND [63]
  Tubacin DD2 of an HDAC6 homology model 4 350 (HDAC1) 5 (prostate cancer)SangtingTao2.5 (acute lymphoblastic leukemia) IC50: 1.2 to 2 µM (acute lymphoblastic leukemia) [57][92][93]
  Tubastatin A His610, His611, Phe679, Phe680 and Tyr782 of HDAC6 15 1093 (HDAC1) 2.5 (unspecified) ND [91][92]
  Tubathian A ND 1.9 5790 (HDAC1) 0.1 (ovarian cancer) ND [94]
Other 3-hydroxypyridine-2-thione (3-HPT) Tyr306 of HDAC8 681 5 (HDAC8) ND Inactive against two prostate cancer cell lines and one acute T cell leukemia cell line [95]
  1-hydroxypyridine-2-thione (1HPT)-6-carboxylic acid DD 150 287 (HDAC1), 4733 (HDAC2), 473 (HDAC4), 233 (HDAC5), 1933 (HDAC7), 22 (HDAC8), 313 (HDAC9) ND CI50: 18 to 75 µM (leukemia) [96]
  Adamantylamino derivative (20a) ND 82 46 (HDAC1), 51 (HDAC4) ND ND [97]
  Mercaptoacetamide derivative (2b) ND 1.3 3615 (HDAC1) 10 (primary rat cortical culture) ND [98]
  Sulfamide derivative (13e) ND 440 >23 (HDAC1) 1 (bladder cancer) ND [99]
Undefined structure
It is important to note that inactivation of HDAC6 protein in mice does not result in abnormal development or major organ problems [94], suggesting that HDAC6 inhibition would have few side effects, unlike pan-HDAC inhibitors.

3.2 HDAC6 inhibitors in solid cancers

Despite the observation of a moderate overexpression of the HDAC6 protein in urothelial cancerous tissues, the inhibition of the protein had limited efficacy compared to the use of inhibitors targeting several HDACs [102]. On the other hand, HDAC6 inhibitors have notable anti-cancer properties in prostate cancer [64], breast cancer [103], melanoma [66], and ovarian cancer [29]. These effects could be explained by the implication of HDAC6 in metastasis formation by epithelial-mesenchymal transition induction via its recruitment by TGFβ [104], in cell migration via α-tubulin deacetylation and in angiogenesis via cortactin deacetylation [105]. In contrast to some selective HDAC6 inhibitors, currently approved pan-HDAC inhibitors failed to show any clinical benefits in solid tumors [94]. The reasons of such therapeutic failures, compared to the treatment of leukemia and lymphoma, are not fully understood; however, some hypotheses have been raised. For example, the hypothesis of some researchers is based on the cellular composition of solid tumors, which tend to arise from more differentiated cells with reduced epigenetic reprogramming capacity [106]. In addition, solid tumor complexity including genomic, epigenomic, and phenotypical changes, can be a part of the explanation [107]. Moreover, the lack of response of solid tumors treated with HDAC inhibitors could be due to the pharmacokinetic profile of those drugs, which generally have a short half-life [108]. For such reasons, some researchers are investigating new methods and routes of administration of these inhibitors. Accordingly, Wang et al. have demonstrated that the use of nanoparticles to administrate HDAC inhibitors allowing a slow release of the drug directly in solid tumors could induce a higher therapeutic efficacy than classic administration routes [109].

3.3. HDAC6 inhibitors in hematological malignancies

Similar to pan-HDAC inhibitors approved for the treatment of hematological cancers, specific HDAC6 inhibitors showed anti-cancer properties in various cancer types such as multiple myeloma [110], chronic lymphocytic leukemia [42],  acute myeloid leukemia (AML) [111], acute lymphoblastic leukemia (ALL) and chronic myeloid leukemia (CML) [doi: 10[112].1016/j.phrs.2020.105058]. 

3.3.1. Nuclear HDAC6 and Its Implication in Leukemia

The cytoplasmic localization of HDAC6 is well described. In leukemia cells, a significant amount of nuclear HDAC6 was revealed, considering the interaction between the nuclear localization sequence (NLS) of HDAC6 and importin-α, which translocates HDAC6 into the nucleus. This region can be heavily acetylated resulting in a reduction of the NLS-importin-α interaction [112113]. Consequently, the presence of HDAC6 in the nucleus of leukemia cells could be explained by red levels of acetylation within the NLS region, compared to other cell types. After nuclear translocation, HDAC6 interacts with nuclear proteins, transcriptional repressors, and transcription factors to regulate gene expression. For example, HDAC6 inhibition has been linked to increased expression of the pro-apoptotic protein BIM in acute myeloid leukemia cells [113114]. Moreover, in a model exhibiting significant nuclear HDAC6 levels, chemical HDAC6 inhibition reduces its nuclear localization and p53-HDAC6 interactions inducing cell cycle arrest and apoptosis via changes of p53 target gene expression [114115]. The specific nuclear localization of HDAC6 in leukemia cells might offer a therapeutic advantage to specifically target those cells.

3.3.2. HDAC6 in CML

3.3.2.1.Degradation of BCR-ABL via Deacetylation of HSP90α by HDAC6 in the Cytoplasm

Although little research exists on HDAC6 in the context of CML, this protein has a function that makes it particularly interesting in the context of such pathology. HDAC6 deacetylates heat shock protein (HSP90)α, which is involved in the stabilization of the oncogenic tyrosine kinase breakpoint cluster region-Abelson (BCR-ABL) protein [113114] protein. In the acetylated form, HSP90α loses its chaperone function, which leads to the degradation of its client proteins by the proteasome (Figure 3A). The importance of the acetylation status of HSP90α in the protein degradation of BCR-ABL makes HDAC6 inhibitors potentially promising molecules for the treatment of CML. Pan-HDAC inhibitors are capable of inducing the inhibition of HDAC6, as well as the downregulation of HDAC6 using si-RNA, which increases the acetylation of HSP90α, and in turn increases the ubiquitination of the BCR-ABL protein, decreasing its expression in K562 cells [115][116][117].

Cancers 12 00318 g006 550
Figure 3. Role of HDAC6 in chronic myeloid leukemia. (A) HDAC6 is implicated in proteasome-dependent protein degradation by its interaction with HSP90α, a chaperone protein which, when deacetylated by HDAC6, is involved in the stabilization of BCR-ABL. In its acetylated (Ac) form, HSP90α loses its chaperone function, which leads to ubiquitination (Ub) and subsequent degradation of BCR-ABL by the proteasome. (B) Imatinib-insensitive chronic myeloid leukemia (CML) leukemic stem cells (LSCs) overexpress HDAC6 compared to CML cells.
3.3.2.2. OverExpression of HDAC6 in CML Stem Cells
LSCs that are not targeted by TKI and are characterized by a capacity for self-renewal play a crucial role in CML relapse. Although HDAC6 is necessary for the repression of genes involved in the differentiation targeted by the Tip60-p400 complex in embryonic stem cells (ESCs) [117118], no study has provided evidence for this in LSCs, more differentiated. In contrast, studies have shown that several proteins in the HDAC family are overexpressed in LCSs of CML. Indeed, SIRT1 is activated by BCR-ABL via STAT5 and its expression is increased in LSCs compared to in CML cells [118119]. Finally, overexpression of isoforms of HDAC (HDAC1, HDAC2, HDAC3, HDAC4, and HDAC5) and in particular HDAC6 was more frequently observed in LSCs (CD34+ CD38-) isolated from patients with CML than in K562 cells [45] (Figure 3B), making it a protein of interest in the search for treatments to prevent relapse in patients with CML.

3.3.3 HDAC6 inhibitors in CLL

HDAC6 is upregulated in CLL patient samples, cell lines, and euTCL1 transgenic mouse models compared to normal controls. Accordingly, this pathology could be an interesting target for selective HDAC6 inhibitors, as genetic silencing of HDAC6 improves the survival of euTCL1 mice. Moreover, the chemical inhibitor ACY738 reduces the proliferation of CLL B cells leading to their apoptosis. Together with ibrutinib, this HDAC6 inhibitor triggers synergistic cell death in vivo [119120]. Beyond the direct effect on pathological B cells, HDAC6 inhibition improves CLL-induced immunosuppression of CLL T cells. HDAC6 inhibitors enhances immune checkpoint blockade in CLL so that combination treatment with ACY738 potentializes the in vivo antitumor effect of anti-PD-1 and anti-PD-L1 antibody treatments with increased cytotoxic CD8+ T cells [119120].

3.3.4 HDAC6 inhibitors in AML

Several HDAC6 inhibitors were assessed as single agents in AML. The HDAC6 inhibitor ST80 shows potent antileukemic activity in myeloid cell lines and primary AML blasts at low micromolar concentrations, leading to preferential acetylation of  a-tubulin [120121]. HDAC inhibitors with a central naphthoquinone structure selectively inhibit HDAC6 in the AML cell line MV4-11, further decreasing mutant FLT-3 protein and constitutively active signal transducer and activators of transcription (STAT)5 levels and reducing extracellular regulated kinase (ERK) phosphorylation [121122]. HDAC inhibitors with the 2-(oxazol-2-yl)phenol moiety as a novel zinc-binding group exhibited selective inhibition against HDAC1 and class IIb HDACs (HDAC6 and HDAC10) in the MV-4-11 AML cells [122123]. The in vivo potency of the selective and orally-available HDAC6 inhibitor N-Hydroxy-4-(2-methoxy-5-(methyl(2-methylquinazolin-4-yl)amino)phenoxy)butanamide 23bb was better against MV4-11 AML cells compared to SAHA or ACY-1215 [doi:10.1021/acs[124].jmedchem.5b01342]. The O-aminobenzamide-based HDAC inhibitor compound 13e down-regulates HDAC6 in MV4-11 cells. 13e induces apoptotic cell death and cycle arrest most likely mediated by a p53-dependent pathway [doi:10[125].1021/acs.jmedchem.8b00136]. The parthenolide-SAHA hybrid compound 26 more potently reduces the viability of the resistant HL-60/ADR AML cell line compared to SAHA, triggering intrinsic apoptosis and reducing the protein expression levels of HDAC1, HDAC6 and the multidrug resistance-associated protein 1 (ABCC1) leading to an intracellular accumulation of drugs [doi:10[126].1016/j.bioorg.2019.03.056]. The HDAC6-selective inhibitor PTG-0861 induces apoptosis in MV4-11 AML cells with limited cytotoxicity against non-malignant cells [doi:10[127].1016/j.ejmech.2020.112411].

In AML, inhibition of HDAC6 was essentially investigated in combination with other pharmacologically active compounds at a pre-clinical level. For instance, a combination of 17-(allylamino)-17-demethoxygeldanamycin (17-AAG), a synthetic derivative of the ansamycin benzoquinone antibiotic geldanamycin, with the HDAC6 inhibitor tubacin reduces the viability of primary AML samples, validating HDAC6 as a HSP90 client protein also in AML and that its hyperacetylation facilitates the anticancer potential of 17-AAG [doi:10[128].1182/blood-2008-03-143644]. LBH-589 and PXD101 inhibit HDAC1 and HDAC6 and synergize with cytarabine to induce cell death in pediatric AML, accompanied by DNA damage induction and increased Bim expression levels [doi:10[129].1371/journal.pone.0017138]. Similarly, Bim protein induction and inhibition of nuclear factor-kappa B (NF-kB) pathway were identified as a mechanistic basis for the synergistic anti-cancer effects of belinostat in combination with the proteasome inhibitor bortezomib in AML and ALL cells [doi:10[130].1111/j.1365-2141.2011.08591.x]. The selective JAK2/HDAC6 dual inhibitor 20a shows excellent in vivo antitumor efficacy in HEL AML mouse xenograft assays and synergizes with the antifungal drug fluconazole [doi:10[131].1021/acs.jmedchem.8b00393]. The selective HDAC6 inhibitor MPT0G211 combined with doxorubicin displays anti-cancer effect by inducing a DNA damage response associated with increased Ku70 acetylation and BAX activation in HL-60 and MOLT-4 AML cell lines. Accordingly, ectopic expression of HDAC6 successively reverses the apoptosis triggered by the combined treatment [doi:10[132].1186/s13148-018-0595-8].

3.3.5 HDAC6 inhibitors in ALL

The HDAC6 inhibitor tubacin enhances the anti-cancer effects of the Na+/K+-ATPase inhibitor ouabain or the proteasome inhibitor MG-13 against pre-B and T ALL cells in vitro and in vivo. These results suggest that selectively targeting HDAC6 alone or in combination with conventional chemotherapeutic drugs could provide a novel approach for ALL therapy [doi:10[133].3109/10428194.2011.570821]. Similarly, belinostat synergizes with the proteasome inhibitor bortezomib to kill ALL cells through Bim up-regulation and NF-kB inhibition. Altogether the perturbation of intracellular microtubular transport network, combined with the interference with protein homeostasis via proteasomal inhibition, could be a general and efficient mechanism explaining the synergistic effect observed [doi:10.1111/j[130].1365-2141.2011.08591.x]. MPT0G211 combined with vincristine interrupts ALL mitosis via interference with microtubular dynamics leading to apoptosis. In vivo, MPT0G211 plus doxorubicin or vincristine reduces tumor growth xenograft models [doi:10[132].1186/s13148-018-0595-8].

Remarkably, it has been shown that the inhibition of HDAC6 using either the pan-HDAC inhibitor trichostatin, the selective HDAC6 inhibitor tubacin, or a genetic knock-down efficiently reduces Notch3 signaling through a post-translational-mediated protein down-regulation, leading to enhanced apoptosis in T-ALL cells and impairing leukemia growth in mice xenografted with T-ALL cell lines and primary human T-ALL cells. These results highlights the therapeutic potential of HDAC6 targeting in Notch3-addicted tumors [doi:10[134].1038/s41388-018-0234-z].

3.3.6 HDAC6 inhibitors in other hematological malignancies

Inhibition of HDAC6 activity increases CD20 levels in B-cell tumor cell lines and malignant patient cells, potentializing the in vivo effect of anti-CD20 monoclonal antibodies like rituximab. Translation of CD20 mRNA is significantly enhanced after HDAC6 inhibition as CD20 mRNA was abundant within the polysomal fraction, indicating a post-transcriptional function of HDAC6. Collectively, these findings suggest HDAC6 inhibition is a rational therapeutic strategy to be implemented in combination therapies with anti-CD20 monoclonal antibodies and open up novel avenues for the clinical use of HDAC6 inhibitors [doi:10.1002/mc.22983][135].

The HDAC6 inhibitor A452 combined with the Bruton's tyrosine kinase inhibitor ibrutinib efficiently kills non-Hodgkin lymphoma cells, including follicular lymphoma [doi:10[135].1002/mc.22983].

The HDAC6 inhibitor KT-531 displays the highest anti-cancer potency against T-cell prolymphocytic leukemia (T-PLL) cells compared to other hematological neoplasms, together with safe differential toxicity compared to non-transformed cell lines. Accordingly, HDAC6 is overexpressed in primary T-PLL patient samples in which KT-531 exerts a potent anti-cancer activity. Moreover, a combination of KT-531 with various approved drugs including bendamustine, idasanutlin, and venetoclax shows promising synergistic effects against T-PLL patient cells [doi:10.1021/acs.jmedchem.1c00420][136].

References

  1. Michael Schnekenburger; Cristina Florean; Mario Dicato; Marc Diederich; Epigenetic alterations as a universal feature of cancer hallmarks and a promising target for personalized treatments. Current Topics in Medicinal Chemistry 2015, 16, 745-776, 10.2174/1568026615666150825141330.
  2. Losson, H.; Schnekenburger, M.; Dicato, M.; Diederich, M. Natural Compound Histone Deacetylase Inhibitors (HDACi): Synergy with Inflammatory Signaling Pathway Modulators and Clinical Applications in Cancer. Molecules 2016, 21, 1608.
  3. Finnin, M.S.; Donigian, J.R.; Cohen, A.; Richon, V.M.; Rifkind, R.A.; Marks, P.A.; Breslow, R.; Pavletich, N.P. Structures of a histone deacetylase homologue bound to the TSA and SAHA inhibitors. Nature 1999, 401, 188–193.
  4. Carafa, V.; Rotili, D.; Forgione, M.; Cuomo, F.; Serretiello, E.; Hailu, G.S.; Jarho, E.; Lahtela-Kakkonen, M.; Mai, A.; Altucci, L. Sirtuin functions and modulation: From chemistry to the clinic. Clin. Epigenetics 2016, 8, 61.
  5. Mei, Z.; Zhang, X.; Yi, J.; Huang, J.; He, J.; Tao, Y. Sirtuins in metabolism, DNA repair and cancer. J. Exp. Clin. Cancer Res. 2016, 35, 182.
  6. Thiagalingam, S.; Cheng, K.H.; Lee, H.J.; Mineva, N.; Thiagalingam, A.; Ponte, J.F. Histone deacetylases: Unique players in shaping the epigenetic histone code. Ann. N. Y. Acad. Sci. 2003, 983, 84–100.
  7. De Ruijter, A.J.; van Gennip, A.H.; Caron, H.N.; Kemp, S.; van Kuilenburg, A.B. Histone deacetylases (HDACs): Characterization of the classical HDAC family. Biochem. J. 2003, 370, 737–749.
  8. Bertos, N.R.; Gilquin, B.; Chan, G.K.; Yen, T.J.; Khochbin, S.; Yang, X.J. Role of the tetradecapeptide repeat domain of human histone deacetylase 6 in cytoplasmic retention. J. Biol. Chem. 2004, 279, 48246–48254.
  9. Li, Y.; Shin, D.; Kwon, S.H. Histone deacetylase 6 plays a role as a distinct regulator of diverse cellular processes. FEBS J. 2013, 280, 775–793.
  10. Zheng, K.; Jiang, Y.; He, Z.; Kitazato, K.; Wang, Y. Cellular defence or viral assist: The dilemma of HDAC6. J. Gen. Virol. 2017, 98, 322–337.
  11. Mortenson, J.B.; Heppler, L.N.; Banks, C.J.; Weerasekara, V.K.; Whited, M.D.; Piccolo, S.R.; Johnson, W.E.; Thompson, J.W.; Andersen, J.L. Histone deacetylase 6 (HDAC6) promotes the pro-survival activity of 14-3-3zeta via deacetylation of lysines within the 14-3-3zeta binding pocket. J. Biol. Chem. 2015, 290, 12487–12496.
  12. Zhang, L.; Liu, S.; Liu, N.; Zhang, Y.; Liu, M.; Li, D.; Seto, E.; Yao, T.P.; Shui, W.; Zhou, J. Proteomic identification and functional characterization of MYH9, Hsc70, and DNAJA1 as novel substrates of HDAC6 deacetylase activity. Protein Cell 2015, 6, 42–54.
  13. Wu, J.Y.; Xiang, S.; Zhang, M.; Fang, B.; Huang, H.; Kwon, O.K.; Zhao, Y.; Yang, Z.; Bai, W.; Bepler, G.; et al. Histone deacetylase 6 (HDAC6) deacetylates extracellular signal-regulated kinase 1 (ERK1) and thereby stimulates ERK1 activity. J. Biol. Chem. 2018, 293, 1976–1993.
  14. Beier, U.H.; Wang, L.; Han, R.; Akimova, T.; Liu, Y.; Hancock, W.W. Histone deacetylases 6 and 9 and sirtuin-1 control Foxp3+ regulatory T cell function through shared and isoform-specific mechanisms. Sci. Signal. 2012, 5, ra45.
  15. Salian-Mehta, S.; Xu, M.; McKinsey, T.A.; Tobet, S.; Wierman, M.E. Novel Interaction of Class IIb Histone Deacetylase 6 (HDAC6) with Class IIa HDAC9 Controls Gonadotropin Releasing Hormone (GnRH) Neuronal Cell Survival and Movement. J. Biol. Chem. 2015, 290, 14045–14056.
  16. Gao, L.; Cueto, M.A.; Asselbergs, F.; Atadja, P. Cloning and functional characterization of HDAC11, a novel member of the human histone deacetylase family. J. Biol. Chem. 2002, 277, 25748–25755.
  17. Medler, T.R.; Craig, J.M.; Fiorillo, A.A.; Feeney, Y.B.; Harrell, J.C.; Clevenger, C.V. HDAC6 Deacetylates HMGN2 to Regulate Stat5a Activity and Breast Cancer Growth. Mol. Cancer Res. 2016, 14, 994–1008.
  18. Chang, Y.W.; Tseng, C.F.; Wang, M.Y.; Chang, W.C.; Lee, C.C.; Chen, L.T.; Hung, M.C.; Su, J.L. Deacetylation of HSPA5 by HDAC6 leads to GP78-mediated HSPA5 ubiquitination at K447 and suppresses metastasis of breast cancer. Oncogene 2016, 35, 1517–1528.
  19. Yang, M.H.; Laurent, G.; Bause, A.S.; Spang, R.; German, N.; Haigis, M.C.; Haigis, K.M. HDAC6 and SIRT2 regulate the acetylation state and oncogenic activity of mutant K-RAS. Mol. Cancer Res. 2013, 11, 1072–1077.
  20. Liu, K.P.; Zhou, D.; Ouyang, D.Y.; Xu, L.H.; Wang, Y.; Wang, L.X.; Pan, H.; He, X.H. LC3B-II deacetylation by histone deacetylase 6 is involved in serum-starvation-induced autophagic degradation. Biochem. Biophys. Res. Commun. 2013, 441, 970–975.
  21. Zhang, M.; Xiang, S.; Joo, H.Y.; Wang, L.; Williams, K.A.; Liu, W.; Hu, C.; Tong, D.; Haakenson, J.; Wang, C.; et al. HDAC6 deacetylates and ubiquitinates MSH2 to maintain proper levels of MutSalpha. Mol. Cell 2014, 55, 31–46.
  22. Perkins, A.; Nelson, K.J.; Parsonage, D.; Poole, L.B.; Karplus, P.A. Peroxiredoxins: Guardians against oxidative stress and modulators of peroxide signaling. Trends Biochem. Sci. 2015, 40, 435–445.
  23. Parmigiani, R.B.; Xu, W.S.; Venta-Perez, G.; Erdjument-Bromage, H.; Yaneva, M.; Tempst, P.; Marks, P.A. HDAC6 is a specific deacetylase of peroxiredoxins and is involved in redox regulation. Proc. Natl. Acad. Sci. USA 2008, 105, 9633–9638.
  24. Moreno-Gonzalo, O.; Mayor, F., Jr.; Sanchez-Madrid, F. HDAC6 at Crossroads of Infection and Innate Immunity. Trends Immunol. 2018, 39, 591–595.
  25. Nakka, K.K.; Chaudhary, N.; Joshi, S.; Bhat, J.; Singh, K.; Chatterjee, S.; Malhotra, R.; De, A.; Santra, M.K.; Dilworth, F.J.; et al. Nuclear matrix-associated protein SMAR1 regulates alternative splicing via HDAC6-mediated deacetylation of Sam68. Proc. Natl. Acad. Sci. USA 2015, 112, E3374–E3383.
  26. Huo, L.; Li, D.; Sun, X.; Shi, X.; Karna, P.; Yang, W.; Liu, M.; Qiao, W.; Aneja, R.; Zhou, J. Regulation of Tat acetylation and transactivation activity by the microtubule-associated deacetylase HDAC6. J. Biol. Chem. 2011, 286, 9280–9286.
  27. Matsuyama, A.; Shimazu, T.; Sumida, Y.; Saito, A.; Yoshimatsu, Y.; Seigneurin-Berny, D.; Osada, H.; Komatsu, Y.; Nishino, N.; Khochbin, S.; et al. In vivo destabilization of dynamic microtubules by HDAC6-mediated deacetylation. EMBO J. 2002, 21, 6820–6831.
  28. Seidel, C.; Schnekenburger, M.; Dicato, M.; Diederich, M. Histone deacetylase 6 in health and disease. Epigenomics 2015, 7, 103–118.
  29. Haakenson, J.; Zhang, X. HDAC6 and ovarian cancer. Int. J. Mol. Sci. 2013, 14, 9514–9535.
  30. Fan, S.J.; Huang, F.I.; Liou, J.P.; Yang, C.R. The novel histone de acetylase 6 inhibitor, MPT0G211, ameliorates tau phosphorylation and cognitive deficits in an Alzheimer’s disease model. Cell Death Dis. 2018, 9, 655.
  31. Lee, S.W.; Yang, J.; Kim, S.Y.; Jeong, H.K.; Lee, J.; Kim, W.J.; Lee, E.J.; Kim, H.S. MicroRNA-26a induced by hypoxia targets HDAC6 in myogenic differentiation of embryonic stem cells. Nucleic Acids Res. 2015, 43, 2057–2073.
  32. Lafarga, V.; Aymerich, I.; Tapia, O.; Mayor, F., Jr.; Penela, P. A novel GRK2/HDAC6 interaction modulates cell spreading and motility. EMBO J. 2012, 31, 856–869.
  33. Williams, K.A.; Zhang, M.; Xiang, S.; Hu, C.; Wu, J.Y.; Zhang, S.; Ryan, M.; Cox, A.D.; Der, C.J.; Fang, B.; et al. Extracellular signal-regulated kinase (ERK) phosphorylates histone deacetylase 6 (HDAC6) at serine 1035 to stimulate cell migration. J. Biol. Chem. 2013, 288, 33156–33170.
  34. Di Fulvio, S.; Azakir, B.A.; Therrien, C.; Sinnreich, M. Dysferlin interacts with histone deacetylase 6 and increases alpha-tubulin acetylation. PLoS ONE 2011, 6, e28563.
  35. Tala, S.X.; Chen, J.; Zhang, L.; Liu, N.; Zhou, J.; Li, D.; Liu, M. Microtubule stabilization by Mdp3 is partially attributed to its modulation of HDAC6 in addition to its association with tubulin and microtubules. PLoS ONE 2014, 9, e90932.
  36. Yan, J.; Seibenhener, M.L.; Calderilla-Barbosa, L.; Diaz-Meco, M.T.; Moscat, J.; Jiang, J.; Wooten, M.W.; Wooten, M.C. SQSTM1/p62 interacts with HDAC6 and regulates deacetylase activity. PLoS ONE 2013, 8, e76016.
  37. Salemi, L.M.; Almawi, A.W.; Lefebvre, K.J.; Schild-Poulter, C. Aggresome formation is regulated by RanBPM through an interaction with HDAC6. Biol. Open 2014, 3, 418–430.
  38. Perez, M.; Santa-Maria, I.; Gomez de Barreda, E.; Zhu, X.; Cuadros, R.; Cabrero, J.R.; Sanchez-Madrid, F.; Dawson, H.N.; Vitek, M.P.; Perry, G.; et al. Tau--an inhibitor of deacetylase HDAC6 function. J. Neurochem. 2009, 109, 1756–1766.
  39. Schofield, A.V.; Gamell, C.; Bernard, O. Tubulin polymerization promoting protein 1 (TPPP1) increases beta-catenin expression through inhibition of HDAC6 activity in U2OS osteosarcoma cells. Biochem. Biophys. Res. Commun. 2013, 436, 571–577.
  40. Batchu, S.N.; Brijmohan, A.S.; Advani, A. The therapeutic hope for HDAC6 inhibitors in malignancy and chronic disease. Clin. Sci. (Lond.) 2016, 130, 987–1003.
  41. Li, T.; Zhang, C.; Hassan, S.; Liu, X.; Song, F.; Chen, K.; Zhang, W.; Yang, J. Histone deacetylase 6 in cancer. J. Hematol. Oncol. 2018, 11, 111.
  42. Maharaj, K.; Powers, J.J.; Achille, A.; Deng, S.; Fonseca, R.; Pabon-Saldana, M.; Quayle, S.N.; Jones, S.S.; Villagra, A.; Sotomayor, E.M.; et al. Silencing of HDAC6 as a therapeutic target in chronic lymphocytic leukemia. Blood Adv. 2018, 2, 3012–3024.
  43. Li, A.; Chen, P.; Leng, Y.; Kang, J. Histone deacetylase 6 regulates the immunosuppressive properties of cancer-associated fibroblasts in breast cancer through the STAT3-COX2-dependent pathway. Oncogene 2018, 37, 5952–5966.
  44. Qian, H.; Chen, Y.; Nian, Z.; Su, L.; Yu, H.; Chen, F.J.; Zhang, X.; Xu, W.; Zhou, L.; Liu, J.; et al. HDAC6-mediated acetylation of lipid droplet-binding protein CIDEC regulates fat-induced lipid storage. J. Clin. Invest. 2017, 127, 1353–1369.
  45. Bamodu, O.A.; Kuo, K.T.; Yuan, L.P.; Cheng, W.H.; Lee, W.H.; Ho, Y.S.; Chao, T.Y.; Yeh, C.T. HDAC inhibitor suppresses proliferation and tumorigenicity of drug-resistant chronic myeloid leukemia stem cells through regulation of hsa-miR-196a targeting BCR/ABL1. Exp. Cell Res. 2018, 370, 519–530.
  46. Cosenza, M.; Pozzi, S. The Therapeutic Strategy of HDAC6 Inhibitors in Lymphoproliferative Disease. Int. J. Mol. Sci. 2018, 19, 2337.
  47. Dehmel, F.; Weinbrenner, S.; Julius, H.; Ciossek, T.; Maier, T.; Stengel, T.; Fettis, K.; Burkhardt, C.; Wieland, H.; Beckers, T. Trithiocarbonates as a novel class of HDAC inhibitors: SAR studies, isoenzyme selectivity, and pharmacological profiles. J. Med. Chem. 2008, 51, 3985–4001.
  48. Inks, E.S.; Josey, B.J.; Jesinkey, S.R.; Chou, C.J. A novel class of small molecule inhibitors of HDAC6. ACS Chem. Biol. 2012, 7, 331–339.
  49. Schafer, S.; Saunders, L.; Eliseeva, E.; Velena, A.; Jung, M.; Schwienhorst, A.; Strasser, A.; Dickmanns, A.; Ficner, R.; Schlimme, S.; et al. Phenylalanine-containing hydroxamic acids as selective inhibitors of class IIb histone deacetylases (HDACs). Bioorg. Med. Chem. 2008, 16, 2011–2033.
  50. Schafer, S.; Saunders, L.; Schlimme, S.; Valkov, V.; Wagner, J.M.; Kratz, F.; Sippl, W.; Verdin, E.; Jung, M. Pyridylalanine-containing hydroxamic acids as selective HDAC6 inhibitors. ChemMedChem 2009, 4, 283–290.
  51. Jochems, J.; Boulden, J.; Lee, B.G.; Blendy, J.A.; Jarpe, M.; Mazitschek, R.; Van Duzer, J.H.; Jones, S.; Berton, O. Antidepressant-like properties of novel HDAC6-selective inhibitors with improved brain bioavailability. Neuropsychopharmacology 2014, 39, 389–400.
  52. Porter, N.J.; Mahendran, A.; Breslow, R.; Christianson, D.W. Unusual zinc-binding mode of HDAC6-selective hydroxamate inhibitors. Proc. Natl. Acad. Sci. USA 2017, 114, 13459–13464.
  53. Krukowski, K.; Ma, J.; Golonzhka, O.; Laumet, G.O.; Gutti, T.; van Duzer, J.H.; Mazitschek, R.; Jarpe, M.B.; Heijnen, C.J.; Kavelaars, A. HDAC6 inhibition effectively reverses chemotherapy-induced peripheral neuropathy. Pain 2017, 158, 1126–1137.
  54. Strebl, M.G.; Campbell, A.J.; Zhao, W.N.; Schroeder, F.A.; Riley, M.M.; Chindavong, P.S.; Morin, T.M.; Haggarty, S.J.; Wagner, F.F.; Ritter, T.; et al. HDAC6 Brain Mapping with [(18)F]Bavarostat Enabled by a Ru-Mediated Deoxyfluorination. ACS Cent. Sci. 2017, 3, 1006–1014.
  55. Wagner, F.F.; Olson, D.E.; Gale, J.P.; Kaya, T.; Weiwer, M.; Aidoud, N.; Thomas, M.; Davoine, E.L.; Lemercier, B.C.; Zhang, Y.L.; et al. Potent and selective inhibition of histone deacetylase 6 (HDAC6) does not require a surface-binding motif. J. Med. Chem. 2013, 56, 1772–1776.
  56. Kozikowski, A.P.; Tapadar, S.; Luchini, D.N.; Kim, K.H.; Billadeau, D.D. Use of the nitrile oxide cycloaddition (NOC) reaction for molecular probe generation: A new class of enzyme selective histone deacetylase inhibitors (HDACIs) showing picomolar activity at HDAC6. J. Med. Chem. 2008, 51, 4370–4373.
  57. Sixto-Lopez, Y.; Bello, M.; Rodriguez-Fonseca, R.A.; Rosales-Hernandez, M.C.; Martinez-Archundia, M.; Gomez-Vidal, J.A.; Correa-Basurto, J. Searching the conformational complexity and binding properties of HDAC6 through docking and molecular dynamic simulations. J. Biomol. Struct. Dyn. 2017, 35, 2794–2814.
  58. Huang, P.; Almeciga-Pinto, I.; Jarpe, M.; van Duzer, J.H.; Mazitschek, R.; Yang, M.; Jones, S.S.; Quayle, S.N. Selective HDAC inhibition by ACY-241 enhances the activity of paclitaxel in solid tumor models. Oncotarget 2017, 8, 2694–2707.
  59. Olsen, C.A.; Ghadiri, M.R. Discovery of potent and selective histone deacetylase inhibitors via focused combinatorial libraries of cyclic alpha3beta-tetrapeptides. J. Med. Chem. 2009, 52, 7836–7846.
  60. Chen, Y.; Lopez-Sanchez, M.; Savoy, D.N.; Billadeau, D.D.; Dow, G.S.; Kozikowski, A.P. A series of potent and selective, triazolylphenyl-based histone deacetylases inhibitors with activity against pancreatic cancer cells and Plasmodium falciparum. J. Med. Chem. 2008, 51, 3437–3448.
  61. Diedrich, D.; Hamacher, A.; Gertzen, C.G.; Alves Avelar, L.A.; Reiss, G.J.; Kurz, T.; Gohlke, H.; Kassack, M.U.; Hansen, F.K. Rational design and diversity-oriented synthesis of peptoid-based selective HDAC6 inhibitors. Chem. Commun. (Camb.) 2016, 52, 3219–3222.
  62. Lin, X.; Chen, W.; Qiu, Z.; Guo, L.; Zhu, W.; Li, W.; Wang, Z.; Zhang, W.; Zhang, Z.; Rong, Y.; et al. Design and synthesis of orally bioavailable aminopyrrolidinone histone deacetylase 6 inhibitors. J. Med. Chem. 2015, 58, 2809–2820.
  63. Blackburn, C.; Barrett, C.; Chin, J.; Garcia, K.; Gigstad, K.; Gould, A.; Gutierrez, J.; Harrison, S.; Hoar, K.; Lynch, C.; et al. Potent histone deacetylase inhibitors derived from 4-(aminomethyl)-N-hydroxybenzamide with high selectivity for the HDAC6 isoform. J. Med. Chem. 2013, 56, 7201–7211.
  64. Seidel, C.; Schnekenburger, M.; Mazumder, A.; Teiten, M.H.; Kirsch, G.; Dicato, M.; Diederich, M. 4-Hydroxybenzoic acid derivatives as HDAC6-specific inhibitors modulating microtubular structure and HSP90alpha chaperone activity against prostate cancer. Biochem. Pharmacol. 2016, 99, 31–52.
  65. Tang, G.; Wong, J.C.; Zhang, W.; Wang, Z.; Zhang, N.; Peng, Z.; Zhang, Z.; Rong, Y.; Li, S.; Zhang, M.; et al. Identification of a novel aminotetralin class of HDAC6 and HDAC8 selective inhibitors. J. Med. Chem. 2014, 57, 8026–8034.
  66. Wang, X.X.; Wan, R.Z.; Liu, Z.P. Recent advances in the discovery of potent and selective HDAC6 inhibitors. Eur. J. Med. Chem. 2018, 143, 1406–1418.
  67. Liang, T.; Hou, X.; Zhou, Y.; Yang, X.; Fang, H. Design, Synthesis, and Biological Evaluation of 2,4-Imidazolinedione Derivatives as HDAC6 Isoform-Selective Inhibitors. ACS Med. Chem. Lett. 2019, 10, 1122–1127.
  68. Kozikowski, A.P.; Chen, Y.; Gaysin, A.; Chen, B.; D’Annibale, M.A.; Suto, C.M.; Langley, B.C. Functional differences in epigenetic modulators-superiority of mercaptoacetamide-based histone deacetylase inhibitors relative to hydroxamates in cortical neuron neuroprotection studies. J. Med. Chem. 2007, 50, 3054–3061.
  69. Lee, H.Y.; Nepali, K.; Huang, F.I.; Chang, C.Y.; Lai, M.J.; Li, Y.H.; Huang, H.L.; Yang, C.R.; Liou, J.P. (N-Hydroxycarbonylbenylamino)quinolines as Selective Histone Deacetylase 6 Inhibitors Suppress Growth of Multiple Myeloma in Vitro and in Vivo. J. Med. Chem. 2018, 61, 905–917.
  70. Shen, S.; Hadley, M.; Ustinova, K.; Pavlicek, J.; Knox, T.; Noonepalle, S.; Tavares, M.T.; Zimprich, C.A.; Zhang, G.; Robers, M.B.; et al. Discovery of a New Isoxazole-3-hydroxamate-Based Histone Deacetylase 6 Inhibitor SS-208 with Antitumor Activity in Syngeneic Melanoma Mouse Models. J. Med. Chem. 2019, 62, 8557–8577.
  71. Vogerl, K.; Ong, N.; Senger, J.; Herp, D.; Schmidtkunz, K.; Marek, M.; Muller, M.; Bartel, K.; Shaik, T.B.; Porter, N.J.; et al. Synthesis and Biological Investigation of Phenothiazine-Based Benzhydroxamic Acids as Selective Histone Deacetylase 6 Inhibitors. J. Med. Chem. 2019, 62, 1138–1166.
  72. Porter, N.J.; Osko, J.D.; Diedrich, D.; Kurz, T.; Hooker, J.M.; Hansen, F.K.; Christianson, D.W. Histone Deacetylase 6-Selective Inhibitors and the Influence of Capping Groups on Hydroxamate-Zinc Denticity. J. Med. Chem. 2018, 61, 8054–8060.
  73. Duan, W.; Li, J.; Inks, E.S.; Chou, C.J.; Jia, Y.; Chu, X.; Li, X.; Xu, W.; Zhang, Y. Design, synthesis, and antitumor evaluation of novel histone deacetylase inhibitors equipped with a phenylsulfonylfuroxan module as a nitric oxide donor. J. Med. Chem. 2015, 58, 4325–4338.
  74. Cho, M.; Choi, E.; Yang, J.S.; Lee, C.; Seo, J.J.; Kim, B.S.; Oh, S.J.; Kim, H.M.; Lee, K.; Park, S.K.; et al. Discovery of pyridone-based histone deacetylase inhibitors: Approaches for metabolic stability. ChemMedChem 2013, 8, 272–279.
  75. Liu, Y.M.; Lee, H.Y.; Lai, M.J.; Pan, S.L.; Huang, H.L.; Kuo, F.C.; Chen, M.C.; Liou, J.P. Pyrimidinedione-mediated selective histone deacetylase 6 inhibitors with antitumor activity in colorectal cancer HCT116 cells. Org. Biomol. Chem. 2015, 13, 10226–10235.
  76. Yu, C.W.; Chang, P.T.; Hsin, L.W.; Chern, J.W. Quinazolin-4-one derivatives as selective histone deacetylase-6 inhibitors for the treatment of Alzheimer’s disease. J. Med. Chem. 2013, 56, 6775–6791.
  77. Heltweg, B.; Dequiedt, F.; Marshall, B.L.; Brauch, C.; Yoshida, M.; Nishino, N.; Verdin, E.; Jung, M. Subtype selective substrates for histone deacetylases. J. Med. Chem. 2004, 47, 5235–5243.
  78. Hideshima, T.; Qi, J.; Paranal, R.M.; Tang, W.; Greenberg, E.; West, N.; Colling, M.E.; Estiu, G.; Mazitschek, R.; Perry, J.A.; et al. Discovery of selective small-molecule HDAC6 inhibitor for overcoming proteasome inhibitor resistance in multiple myeloma. Proc. Natl. Acad. Sci. USA 2016, 113, 13162–13167.
  79. Sellmer, A.; Stangl, H.; Beyer, M.; Grunstein, E.; Leonhardt, M.; Pongratz, H.; Eichhorn, E.; Elz, S.; Striegl, B.; Jenei-Lanzl, Z.; et al. Marbostat-100 Defines a New Class of Potent and Selective Antiinflammatory and Antirheumatic Histone Deacetylase 6 Inhibitors. J. Med. Chem. 2018, 61, 3454–3477.
  80. Lee, H.Y.; Tsai, A.C.; Chen, M.C.; Shen, P.J.; Cheng, Y.C.; Kuo, C.C.; Pan, S.L.; Liu, Y.M.; Liu, J.F.; Yeh, T.K.; et al. Azaindolylsulfonamides, with a more selective inhibitory effect on histone deacetylase 6 activity, exhibit antitumor activity in colorectal cancer HCT116 cells. J. Med. Chem. 2014, 57, 4009–4022.
  81. Mackwitz, M.K.W.; Hamacher, A.; Osko, J.D.; Held, J.; Scholer, A.; Christianson, D.W.; Kassack, M.U.; Hansen, F.K. Multicomponent Synthesis and Binding Mode of Imidazo[1,2 -a]pyridine-Capped Selective HDAC6 Inhibitors. Org. Lett. 2018, 20, 3255–3258.
  82. Lee, J.H.; Yao, Y.; Mahendran, A.; Ngo, L.; Venta-Perez, G.; Choy, M.L.; Breslow, R.; Marks, P.A. Creation of a histone deacetylase 6 inhibitor and its biological effects [corrected]. Proc. Natl. Acad. Sci. USA 2015, 112, 12005–12010.
  83. Lee, J.H.; Mahendran, A.; Yao, Y.; Ngo, L.; Venta-Perez, G.; Choy, M.L.; Kim, N.; Ham, W.S.; Breslow, R.; Marks, P.A. Development of a histone deacetylase 6 inhibitor and its biological effects. Proc. Natl. Acad. Sci. USA 2013, 110, 15704–15709.
  84. Hai, Y.; Christianson, D.W. Histone deacetylase 6 structure and molecular basis of catalysis and inhibition. Nat. Chem. Biol. 2016, 12, 741–747.
  85. Yang, Z.; Wang, T.; Wang, F.; Niu, T.; Liu, Z.; Chen, X.; Long, C.; Tang, M.; Cao, D.; Wang, X.; et al. Discovery of Selective Histone Deacetylase 6 Inhibitors Using the Quinazoline as the Cap for the Treatment of Cancer. J. Med. Chem. 2016, 59, 1455–1470.
  86. Bergman, J.A.; Woan, K.; Perez-Villarroel, P.; Villagra, A.; Sotomayor, E.M.; Kozikowski, A.P. Selective histone deacetylase 6 inhibitors bearing substituted urea linkers inhibit melanoma cell growth. J. Med. Chem. 2012, 55, 9891–9899.
  87. Senger, J.; Melesina, J.; Marek, M.; Romier, C.; Oehme, I.; Witt, O.; Sippl, W.; Jung, M. Synthesis and Biological Investigation of Oxazole Hydroxamates as Highly Selective Histone Deacetylase 6 (HDAC6) Inhibitors. J. Med. Chem. 2016, 59, 1545–1555.
  88. Santo, L.; Hideshima, T.; Kung, A.L.; Tseng, J.C.; Tamang, D.; Yang, M.; Jarpe, M.; van Duzer, J.H.; Mazitschek, R.; Ogier, W.C.; et al. Preclinical activity, pharmacodynamic, and pharmacokinetic properties of a selective HDAC6 inhibitor, ACY-1215, in combination with bortezomib in multiple myeloma. Blood 2012, 119, 2579–2589.
  89. Wang, F.; Zhong, B.W.; Zhao, Z.R. ACY 1215, a histone deacetylase 6 inhibitor, inhibits cancer cell growth in melanoma. J. Biol. Regul. Homeost. Agents 2018, 32, 851–858.
  90. Zhang, I.; Beus, M.; Stochaj, U.; Le, P.U.; Zorc, B.; Rajic, Z.; Petrecca, K.; Maysinger, D. Inhibition of glioblastoma cell proliferation, invasion, and mechanism of action of a novel hydroxamic acid hybrid molecule. Cell Death Discov 2018, 4, 41.
  91. Dong, J.; Zheng, N.; Wang, X.; Tang, C.; Yan, P.; Zhou, H.B.; Huang, J. A novel HDAC6 inhibitor exerts an anti-cancer effect by triggering cell cycle arrest and apoptosis in gastric cancer. Eur. J. Pharmacol. 2018, 828, 67–79.
  92. Butler, K.V.; Kalin, J.; Brochier, C.; Vistoli, G.; Langley, B.; Kozikowski, A.P. Rational design and simple chemistry yield a superior, neuroprotective HDAC6 inhibitor, tubastatin A. J. Am. Chem. Soc. 2010, 132, 10842–10846.
  93. Aldana-Masangkay, G.I.; Rodriguez-Gonzalez, A.; Lin, T.; Ikeda, A.K.; Hsieh, Y.T.; Kim, Y.M.; Lomenick, B.; Okemoto, K.; Landaw, E.M.; Wang, D.; et al. Tubacin suppresses proliferation and induces apoptosis of acute lymphoblastic leukemia cells. Leuk. Lymphoma 2011, 52, 1544–1555.
  94. Depetter, Y.; Geurs, S.; De Vreese, R.; Goethals, S.; Vandoorn, E.; Laevens, A.; Steenbrugge, J.; Meyer, E.; de Tullio, P.; Bracke, M.; et al. Selective pharmacological inhibitors of HDAC6 reveal biochemical activity but functional tolerance in cancer models. Int. J. Cancer 2019, 145, 735–747.
  95. Patil, V.; Sodji, Q.H.; Kornacki, J.R.; Mrksich, M.; Oyelere, A.K. 3-Hydroxypyridin-2-thione as novel zinc binding group for selective histone deacetylase inhibition. J. Med. Chem. 2013, 56, 3492–3506.
  96. Muthyala, R.; Shin, W.S.; Xie, J.; Sham, Y.Y. Discovery of 1-hydroxypyridine-2-thiones as selective histone deacetylase inhibitors and their potential application for treating leukemia. Bioorg. Med. Chem. Lett. 2015, 25, 4320–4324.
  97. Itoh, Y.; Suzuki, T.; Kouketsu, A.; Suzuki, N.; Maeda, S.; Yoshida, M.; Nakagawa, H.; Miyata, N. Design, synthesis, structure--selectivity relationship, and effect on human cancer cells of a novel series of histone deacetylase 6-selective inhibitors. J. Med. Chem. 2007, 50, 5425–5438.
  98. Segretti, M.C.; Vallerini, G.P.; Brochier, C.; Langley, B.; Wang, L.; Hancock, W.W.; Kozikowski, A.P. Thiol-Based Potent and Selective HDAC6 Inhibitors Promote Tubulin Acetylation and T-Regulatory Cell Suppressive Function. ACS Med. Chem. Lett. 2015, 6, 1156–1161.
  99. Wahhab, A.; Smil, D.; Ajamian, A.; Allan, M.; Chantigny, Y.; Therrien, E.; Nguyen, N.; Manku, S.; Leit, S.; Rahil, J.; et al. Sulfamides as novel histone deacetylase inhibitors. Bioorg. Med. Chem. Lett. 2009, 19, 336–340.
  100. Choi, E.W.; Song, J.W.; Ha, N.; Choi, Y.I.; Kim, S. CKD-506, a novel HDAC6-selective inhibitor, improves renal outcomes and survival in a mouse model of systemic lupus erythematosus. Sci. Rep. 2018, 8, 17297.
  101. Schnekenburger, M.; Florean, C.; Dicato, M.; Diederich, M. Epigenetic alterations as a universal feature of cancer hallmarks and a promising target for personalized treatments. Curr. Top. Med. Chem. 2016, 16, 745–776.
  102. Rosik, L.; Niegisch, G.; Fischer, U.; Jung, M.; Schulz, W.A.; Hoffmann, M.J. Limited efficacy of specific HDAC6 inhibition in urothelial cancer cells. Cancer Biol. Ther. 2014, 15, 742–757.
  103. Hsieh, Y.L.; Tu, H.J.; Pan, S.L.; Liou, J.P.; Yang, C.R. Anti-metastatic activity of MPT0G211, a novel HDAC6 inhibitor, in human breast cancer cells in vitro and in vivo. Biochim. Biophys. Acta Mol. Cell Res. 2019, 1866, 992–1003.
  104. Shan, B.; Yao, T.P.; Nguyen, H.T.; Zhuo, Y.; Levy, D.R.; Klingsberg, R.C.; Tao, H.; Palmer, M.L.; Holder, K.N.; Lasky, J.A. Requirement of HDAC6 for transforming growth factor-beta1-induced epithelial-mesenchymal transition. J. Biol. Chem. 2008, 283, 21065–21073.
  105. Kaluza, D.; Kroll, J.; Gesierich, S.; Yao, T.P.; Boon, R.A.; Hergenreider, E.; Tjwa, M.; Rossig, L.; Seto, E.; Augustin, H.G.; et al. Class IIb HDAC6 regulates endothelial cell migration and angiogenesis by deacetylation of cortactin. EMBO J. 2011, 30, 4142–4156.
  106. Morel, D.; Jeffery, D.; Aspeslagh, S.; Almouzni, G.; Postel-Vinay, S. Combining epigenetic drugs with other therapies for solid tumours—Past lessons and future promise. Nat. Rev. Clin. Oncol. 2020, 17, 91–107.
  107. Valdespino, V.; Valdespino, P.M. Potential of epigenetic therapies in the management of solid tumors. Cancer Manag. Res. 2015, 7, 241–251.
  108. Grassadonia, A.; Cioffi, P.; Simiele, F.; Iezzi, L.; Zilli, M.; Natoli, C. Role of Hydroxamate-Based Histone Deacetylase Inhibitors (Hb-HDACIs) in the Treatment of Solid Malignancies. Cancers 2013, 5, 919–942.
  109. Wang, E.C.; Min, Y.; Palm, R.C.; Fiordalisi, J.J.; Wagner, K.T.; Hyder, N.; Cox, A.D.; Caster, J.M.; Tian, X.; Wang, A.Z. Nanoparticle formulations of histone deacetylase inhibitors for effective chemoradiotherapy in solid tumors. Biomaterials 2015, 51, 208–215.
  110. Brindisi, M.; Saraswati, A.P.; Brogi, S.; Gemma, S.; Butini, S.; Campiani, G. Old but Gold: Tracking the New Guise of Histone Deacetylase 6 (HDAC6) Enzyme as a Biomarker and Therapeutic Target in Rare Diseases. J. Med. Chem. 2020, 63, 23–39.
  111. Hackanson, B.; Rimmele, L.; Benkisser, M.; Abdelkarim, M.; Fliegauf, M.; Jung, M.; Lubbert, M. HDAC6 as a target for antileukemic drugs in acute myeloid leukemia. Leuk. Res. 2012, 36, 1055–1062.
  112. Liu, Y.; Peng, L.; Seto, E.; Huang, S.; Qiu, Y. Modulation of histone deacetylase 6 (HDAC6) nuclear import and tubulin deacetylase activity through acetylation. J. Biol. Chem. 2012, 287, 29168–29174.Hélène Losson; Sruthi Reddy Gajulapalli; Manon Lernoux; Jin-Young Lee; Aloran Mazumder; Déborah Gérard; Carole Seidel; HyungGu Hahn; Christo Christov; Mario Dicato; et al.Gilbert KirschByung Woo HanMichael SchnekenburgerMarc Diederich The HDAC6 inhibitor 7b induces BCR-ABL ubiquitination and downregulation and synergizes with imatinib to trigger apoptosis in chronic myeloid leukemia. Pharmacological Research 2020, 160, 105058, 10.1016/j.phrs.2020.105058.
  113. Kramer, O.H.; Mahboobi, S.; Sellmer, A. Drugging the HDAC6-HSP90 interplay in malignant cells. Trends Pharmacol. Sci. 2014, 35, 501–509.Liu, Y.; Peng, L.; Seto, E.; Huang, S.; Qiu, Y. Modulation of histone deacetylase 6 (HDAC6) nuclear import and tubulin deacetylase activity through acetylation. J. Biol. Chem. 2012, 287, 29168–29174.
  114. Ryu, H.W.; Shin, D.H.; Lee, D.H.; Choi, J.; Han, G.; Lee, K.Y.; Kwon, S.H. HDAC6 deacetylates p53 at lysines 381/382 and differentially coordinates p53-induced apoptosis. Cancer Lett. 2017, 391, 162–171.Kramer, O.H.; Mahboobi, S.; Sellmer, A. Drugging the HDAC6-HSP90 interplay in malignant cells. Trends Pharmacol. Sci. 2014, 35, 501–509.
  115. Bali, P.; Pranpat, M.; Bradner, J.; Balasis, M.; Fiskus, W.; Guo, F.; Rocha, K.; Kumaraswamy, S.; Boyapalle, S.; Atadja, P.; et al. Inhibition of histone deacetylase 6 acetylates and disrupts the chaperone function of heat shock protein 90: A novel basis for antileukemia activity of histone deacetylase inhibitors. J. Biol. Chem. 2005, 280, 26729–26734.Ryu, H.W.; Shin, D.H.; Lee, D.H.; Choi, J.; Han, G.; Lee, K.Y.; Kwon, S.H. HDAC6 deacetylates p53 at lysines 381/382 and differentially coordinates p53-induced apoptosis. Cancer Lett. 2017, 391, 162–171.
  116. Rao, R.; Fiskus, W.; Yang, Y.; Lee, P.; Joshi, R.; Fernandez, P.; Mandawat, A.; Atadja, P.; Bradner, J.E.; Bhalla, K. HDAC6 inhibition enhances 17-AAG--mediated abrogation of hsp90 chaperone function in human leukemia cells. Blood 2008, 112, 1886–1893.Bali, P.; Pranpat, M.; Bradner, J.; Balasis, M.; Fiskus, W.; Guo, F.; Rocha, K.; Kumaraswamy, S.; Boyapalle, S.; Atadja, P.; et al. Inhibition of histone deacetylase 6 acetylates and disrupts the chaperone function of heat shock protein 90: A novel basis for antileukemia activity of histone deacetylase inhibitors. J. Biol. Chem. 2005, 280, 26729–26734.
  117. Chen, P.B.; Hung, J.H.; Hickman, T.L.; Coles, A.H.; Carey, J.F.; Weng, Z.; Chu, F.; Fazzio, T.G. Hdac6 regulates Tip60-p400 function in stem cells. Elife 2013, 2, e01557.Rao, R.; Fiskus, W.; Yang, Y.; Lee, P.; Joshi, R.; Fernandez, P.; Mandawat, A.; Atadja, P.; Bradner, J.E.; Bhalla, K. HDAC6 inhibition enhances 17-AAG--mediated abrogation of hsp90 chaperone function in human leukemia cells. Blood 2008, 112, 1886–1893.
  118. Kuo, Y.H.; Qi, J.; Cook, G.J. Regain control of p53: Targeting leukemia stem cells by isoform-specific HDAC inhibition. Exp. Hematol. 2016, 44, 315–321.Chen, P.B.; Hung, J.H.; Hickman, T.L.; Coles, A.H.; Carey, J.F.; Weng, Z.; Chu, F.; Fazzio, T.G. Hdac6 regulates Tip60-p400 function in stem cells. Elife 2013, 2, e01557.
  119. Kamira Maharaj; John J. Powers; Melanie Mediavilla-Varela; Alex Achille; Wael Gamal; Steven Quayle; Simon S. Jones; Eva Sahakian; Javier Pinilla-Ibarz; HDAC6 Inhibition Alleviates CLL-Induced T-Cell Dysfunction and Enhances Immune Checkpoint Blockade Efficacy in the Eμ-TCL1 Model. Frontiers in Immunology 2020, 11, -, 10.3389/fimmu.2020.590072.Kuo, Y.H.; Qi, J.; Cook, G.J. Regain control of p53: Targeting leukemia stem cells by isoform-specific HDAC inhibition. Exp. Hematol. 2016, 44, 315–321.
  120. Björn Hackanson; Leander Rimmele; Marco Benkißer; Mahmoud Abdelkarim; Manfred Fliegauf; Manfred Jung; Michael Lübbert; HDAC6 as a target for antileukemic drugs in acute myeloid leukemia. LKamira Maharaj; John J. Powers; Melanie Mediavilla-Varela; Alex Achille; Wael Gamal; Steven Quayle; Simon S. Jones; Eva Sahakian; Javier Pinilla-Ibarz; HDAC6 Inhibition Alleviates CLL-Induced T-Cell Dysfunction and Enhances Immune Checkpoint Blockade Efficacy in the Eμ-TCL1 Model. Frontieukemrs ia Research n Immunology 2012, 36, 1055-1062, 10.1016/j.leukres.2012.02.026.0, 11, -, 10.3389/fimmu.2020.590072.
  121. Elizabeth S. Inks; Benjamin J. Josey; Sean R. Jesinkey; C. James Chou; A Novel Class of Small Molecule Inhibitors of HDAC6. ACS ChBjörn Hackanson; Leander Rimmele; Marco Benkißer; Mahmoud Abdelkarim; Manfred Fliegauf; Manfred Jung; Michael Lübbert; HDAC6 as a target for antileukemic drugs in acute myeloid leukemia. Leukemical Biology Research 2011, 7, 331-339, 10.1021/cb200134p.2, 36, 1055-1062, 10.1016/j.leukres.2012.02.026.
  122. Youxuan Li; Patrick M. Woster; Discovery of a new class of histone deacetylase inhibitors with a novel zinc binding group. MedElizabeth S. Inks; Benjamin J. Josey; Sean R. Jesinkey; C. James Chou; A Novel Class of Small Molecule Inhibitors of HDAC6. ACS ChemCical Biolomm gy 20114, 6, 613-618, 10.1039/c4md00401a., 7, 331-339, 10.1021/cb200134p.
  123. Youxuan Li; Patrick M. Woster; Discovery of a new class of histone deacetylase inhibitors with a novel zinc binding group. MedChemComm 2014, 6, 613-618, 10.1039/c4md00401a.
  124. Zhuang Yang; Taijin Wang; Fang Wang; Ting Niu; Zhuowei Liu; Xiaoxin Chen; Chaofeng Long; Minghai Tang; Dong Cao; Xiaoyan Wang; et al.Wei XiangYuyao YiLiang MaJingsong YouLijuan Chen Discovery of Selective Histone Deacetylase 6 Inhibitors Using the Quinazoline as the Cap for the Treatment of Cancer. Journal of Medicinal Chemistry 2015, 59, 1455-1470, 10.1021/acs.jmedchem.5b01342.
  125. Xiaoyang Li; Yuri K. Peterson; Elizabeth S. Inks; Richard A. Himes; Jiaying Li; Yingjie Zhang; Xiujie Kong; C. James Chou; Class I HDAC Inhibitors Display Different Antitumor Mechanism in Leukemia and Prostatic Cancer Cells Depending on Their p53 Status. Journal of Medicinal Chemistry 2018, 61, 2589-2603, 10.1021/acs.jmedchem.8b00136.
  126. Weizhi Ge; Zhongquan Liu; Yu Sun; Tianpeng Wang; Hongyu Guo; Xinyi Chen; Shengzu Li; Mengmeng Wang; Yue Chen; Yahui Ding; et al.Quan Zhang Design and synthesis of parthenolide-SAHA hybrids for intervention of drug-resistant acute myeloid leukemia. Bioorganic Chemistry 2019, 87, 699-713, 10.1016/j.bioorg.2019.03.056.
  127. Justyna M. Gawel; Andrew E. Shouksmith; Yasir S. Raouf; Nabanita Nawar; Krimo Toutah; Shazreh Bukhari; Pimyupa Manaswiyoungkul; Olasunkanmi O. Olaoye; Johan Israelian; Tudor B. Radu; et al.Aaron D. CabralDiana SinaAbootaleb SedighiElvin D. de AraujoPatrick T. Gunning PTG-0861: A novel HDAC6-selective inhibitor as a therapeutic strategy in acute myeloid leukaemia. European Journal of Medicinal Chemistry 2020, 201, 112411, 10.1016/j.ejmech.2020.112411.
  128. Rekha Rao; Warren Fiskus; Yonghua Yang; Pearl Lee; Rajeshree Joshi; Pravina Fernandez; Aditya Mandawat; Peter Atadja; James E. Bradner; Kapil Bhalla; et al. HDAC6 inhibition enhances 17-AAG–mediated abrogation of hsp90 chaperone function in human leukemia cells. Blood 2008, 112, 1886-1893, 10.1182/blood-2008-03-143644.
  129. Xuelian Xu; Chengzhi Xie; Holly Edwards; Hui Zhou; Steven A. Buck; Yubin Ge; Inhibition of Histone Deacetylases 1 and 6 Enhances Cytarabine-Induced Apoptosis in Pediatric Acute Myeloid Leukemia Cells. PLOS ONE 2011, 6, e17138, 10.1371/journal.pone.0017138.
  130. Yun Dai; Shuang Chen; Li Wang; Xin-Yan Pei; Lora B. Kramer; Paul Dent; Steven Grant; Bortezomib interacts synergistically with belinostat in human acute myeloid leukaemia and acute lymphoblastic leukaemia cells in association with perturbations in NF-κB and Bim. British Journal of Haematology 2011, 153, 222-235, 10.1111/j.1365-2141.2011.08591.x.
  131. Yahui Huang; Guoqiang Dong; Huanqiu Li; Na Liu; Wannian Zhang; Chunquan Sheng; Discovery of Janus Kinase 2 (JAK2) and Histone Deacetylase (HDAC) Dual Inhibitors as a Novel Strategy for the Combinational Treatment of Leukemia and Invasive Fungal Infections. Journal of Medicinal Chemistry 2018, 61, 6056-6074, 10.1021/acs.jmedchem.8b00393.
  132. Huang-Ju Tu; Yi-Jyun Lin; Min-Wu Chao; Ting-Yi Sung; Yi-Wen Wu; Yi-Ying Chen; Mei-Hsiang Lin; Jing-Ping Liou; Shiow-Lin Pan; Chia-Ron Yang; et al. The anticancer effects of MPT0G211, a novel HDAC6 inhibitor, combined with chemotherapeutic agents in human acute leukemia cells. Clinical Epigenetics 2018, 10, 1-13, 10.1186/s13148-018-0595-8.
  133. Grace I. Aldana-Masangkay; Agustin Rodriguez-Gonzalez; Tara Lin; Alan K. Ikeda; Yao-Te Hsieh; Yong-Mi Kim; Brett Lomenick; Kazuo Okemoto; Elliot M. Landaw; Dongpeng Wang; et al.Ralph MazitschekJames E. BradnerKathleen M. Sakamoto Tubacin suppresses proliferation and induces apoptosis of acute lymphoblastic leukemia cells. Leukemia & Lymphoma 2011, 52, 1544-1555, 10.3109/10428194.2011.570821.
  134. Marica Pinazza; Margherita Ghisi; Sonia Anna Minuzzo; Valentina Agnusdei; Gianluca Fossati; Vincenzo Ciminale; Laura Pezzè; Yari Ciribilli; Giorgia Pilotto; Carolina Venturoli; et al.Alberto AmadoriStefano Indraccolo Histone deacetylase 6 controls Notch3 trafficking and degradation in T-cell acute lymphoblastic leukemia cells. Oncogene 2018, 37, 3839-3851, 10.1038/s41388-018-0234-z.
  135. Ng Hoon Lee; Go Woon Kim; So Hee Kwon; The HDAC6‐selective inhibitor is effective against non‐Hodgkin lymphoma and synergizes with ibrutinib in follicular lymphoma. Molecular Carcinogenesis 2019, 58, 944-956, 10.1002/mc.22983.
  136. Krimo Toutah; Nabanita Nawar; Sanna Timonen; Helena Sorger; Yasir S. Raouf; Shazreh Bukhari; Jana von Jan; Aleksandr Ianevski; Justyna M. Gawel; Olasunkanmi O. Olaoye; et al.Mulu GeletuAyah AbdeldayemJohan IsraelianTudor B. RaduAbootaleb SedighiMuzaffar N. BhattiMuhammad Murtaza HassanPimyupa ManaswiyoungkulAndrew E. ShouksmithHeidi A. NeubauerElvin D. de AraujoTero AittokallioOliver H. KrämerRichard MorigglSatu MustjokiMarco HerlingPatrick T. Gunning Development of HDAC Inhibitors Exhibiting Therapeutic Potential in T-Cell Prolymphocytic Leukemia. Journal of Medicinal Chemistry 2021, 64, 8486-8509, 10.1021/acs.jmedchem.1c00420.
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