Chloroplasts: Comparison
Please note this is a comparison between Version 1 by Shunping Yan and Version 2 by Rita Xu.

The chloroplast is a semi-autonomous organelle with its own genome. The expression of chloroplast genes depends on both chloroplasts and the nucleus. In chloroplast, NAC102 associates with the chloroplast genome, interacts with chloroplast RNA polymerases, and regulates chloroplast gene expression.

  • chloroplast
  • gene expression
  • NAC102
  • nucleus
  • transcription factor

1. Introduction

One of the major differences between plants and animals is that plants contain chloroplasts, which carry out photosynthesis, produce various metabolites, and sense external cues. Chloroplasts are believed to derive from cyanobacterium in an endosymbiotic event. During evolution, most bacteria genes were transferred to the host nucleus and only a small portion of genes were retained on the own genome [1][2][1,2]. Therefore, the crosstalk between chloroplasts and the nucleus is essential to maintain cellular homeostasis, which is achieved through anterograde (nucleus-to-chloroplasts) and retrograde (chloroplasts-to-nucleus) signaling [3][4][3,4].
As a semi-autonomous organelle, the gene expression in chloroplasts is controlled by both chloroplasts and the nucleus [2]. For example, both plastid-encoded RNA polymerase (PEP) and the nuclear-encoded RNA polymerase (NEP) contribute to chloroplast gene expression [5][6][5,6]. While the expression of photosynthetic genes, such as psbA, psbB, and rbcL, is preferentially dependent on PEP, the expression of the housekeeping genes, including ribosomal RNAs and the core subunits of the PEP, is dependent on NEP [7][8][9][7,8,9]. NEP is a phage-type RNA polymerase with a single subunit, encoded by two nuclear genes, rpoTp and rpoTmp, in Arabidopsis. PEP is a bacteria-type RNA polymerase that consists of four core subunits (α, β, β′, and β″), encoded by rpoA, rpoB, rpoC1, and rpoC2 in the chloroplast genome, and a promoter-recognizing sigma factor (σ) encoded by the nuclear genes [10][11][10,11]. Furthermore, PEP forms a big complex with many PEP-associated proteins (PAPs) to regulate gene expression. All PAPs are encoded by the nuclear genome [12][13][14][12,13,14].
In addition to PAPs, many other nucleus-encoded proteins have been reported to regulate chloroplast gene expression at the transcriptional level or the post-transcriptional level (including RNA splicing, RNA editing, and translation) [13][15][16][13,15,16]. Although much progress has been made towards understanding their functions in post-transcriptional regulation, how they regulate chloroplast gene expression at the transcriptional level is less well-understood.
In plants, the NAC (NAM, ATAF, and CUC) family is one of the largest plant-specific transcription factors and plays important roles in plant development and stress responses [17][18][17,18]. Previous studies have revealed that the transcription factor NAC102 is involved in oxidative stress responses and the nac102 mutant is sensitive to excessive light [19][20][21][19,20,21].

2. Plant Materials and Growth Condition

All Arabidopsis thaliana used in this study were in the Columbia (Col-0) background. The nac102 mutant (SALK_030702) was obtained from ABRC. The transgenic lines were generated by the floral dip method [22]. Arabidopsis was grown on half-strength (1/2) Murashige and Skoog (MS) medium or in soil under long-day conditions (16 h of light and 8 h of dark) at 22 ± 2 °C.

3. Vector Constructions

For localization analysis of NAC102, the coding sequence of enhanced yellow fluorescent protein (eYFP) was fused to the C-terminus of the NAC102 fragment (NAC102-eYFP) using fusion PCR. The NAC102ΔN43-eYFP was generated by using NAC102-eYFP as a template. To generate NAC102N43-eYFP, NAC102N60-eYFP, and NAC102N80-eYFP, the 43, 60 and 80 aa N-terminal parts of NAC102 were cloned and fused to eYFP, respectively. To generate NAC102-eYFPNES, the coding sequence of nuclear export sequence (NES) (CLPPLERLTLD) [23] was further fused C-terminally to NAC102-eYFP (Figure 1). To generate TRXz-CFP, the coding sequence of cyan fluorescent protein (CFP) was fused to the C-terminus of TRXz. All fusion fragments were inserted into pFGC5941 at the NcoI and XbaI sites. For Y2H assays, the coding sequence of NAC102 was inserted into pGBKT7 at the NdeI and PstI sites. The coding sequences of the subunits of PEP and NEP were inserted into pGADT7 at the NdeI and PstI sites. All recombination reactions were performed using a Lightening Cloning kit (Biodragon, Beijing, China). The primers used for vector constructions are listed in the Supplementary Materials, Table S1.
Figure 1. A schematic diagram of NAC102 fusion proteins.

4. NAC102 Localizes in Both Chloroplasts and Nucleus

In Arabidopsis thaliana, there are 105 NAC transcription factors [24][26], among which NAC102 (AT5G63790) is predicted to localize in both chloroplast and nucleus according to TAIR (; 1 June 2016). To our knowledge, no transcription factors have been reported to localize in the chloroplast. Therefore, we decided to study NAC102 further. NAC102 has two alternative transcripts, AT5G63790.1 and AT5G63790.2. As shown in Figure S1, compared to AT5G63790.1, AT5G63790.2 encodes a protein with an additional 10 amino acids at the N-terminus. To confirm the subcellular localization of NAC102, both NAC102 variants were fused with enhanced yellow fluorescent protein (eYFP) and were transiently expressed in Arabidopsis protoplasts. The CFP-tagged E2Fa transcription factor (E2Fa-CFP) was used as the nucleus marker and the chlorophyll fluorescence was used as the chloroplast marker. As shown in Figure 2, both AT5G63790.1-eYFP and AT5G63790.2-eYFP localized in chloroplast and nucleus. In the following study, we only used AT5G63790.1. To further confirm its localization, we generated the transgenic Arabidopsis expressing NAC102-eYFP (AT5G63790.1) driven by the 35S promoter (35S:NAC102-eYFP). Consistently, the eYFP fluorescence was detected in both chloroplast and nucleus (Figure 2C).
Figure 2. NAC102 localizes in both the nucleus and the chloroplast. (A,B) The localization of NAC102-eYFP when it was transiently expressed in Arabidopsis protoplasts. E2Fa-CFP was used as the nucleus marker and the chlorophyll fluorescence was used as the chloroplast marker. Both isoforms of NAC102, AT5G63790.1 (A) and AT5G63790.2 (B), were shown. (C) The localization of NAC102-eYFP in epidermal cells derived from NAC102 (AT5G63790.1) transgenic lines. The nucleus was stained with DAPI. The pictures were captured using confocal microscopy. Bars = 10 µm.

5. The N-Terminal Sequence of NAC102 Is Necessary and Sufficient for Its Chloroplast Localization

Most nucleus-encoded chloroplast proteins contain transit peptides at their N-terminus to direct them to the chloroplast. To map the transit peptide of NAC102, we performed a prediction using the ChloroP server [25][27]. However, no transit peptide was identified. Interestingly, when the NAC102 orthologs from other plant species were aligned, we found that the N-terminal 43 amino acid residues (N43) of NAC102 from Arabidopsis thaliana is very unique (Figure S2). We speculated that N43 is necessary for its chloroplast localization. To test this hypothesis, we transiently expressed the truncated NAC102-eYFP without N43 (NAC102ΔN43-eYFP) in Arabidopsis protoplasts. In support of the hypothesis, the NAC102ΔN43-eYFP was only detected in the nucleus (Figure 3A). To investigate whether N43 is sufficient for its chloroplast localization, we fused eYFP with N43 (N43-eYFP) and examined its localization. As shown in Figure 3B, the N43-eYFP could not localize in chloroplasts, suggesting that more amino acid residues are required for the chloroplast localization of NAC102. Therefore, we investigated the localization of eYFP fused to N-terminal 60 or 80 amino acid residues (N60 or N80). We found that the N80-eYFP but not N60-eYFP could localize in chloroplasts (Figure 3C,D). These results suggested that the N-terminal sequence of NAC102 is necessary and sufficient for its chloroplast localization.
Figure 3. The effects of the N-terminal sequence of NAC102 on its chloroplast localization. The fusion proteins were transiently expressed in Arabidopsis protoplasts. NAC102ΔN43-eYFP (A) represented the truncated NAC102-eYFP without N-terminal 43 amino acid residues (N43); NAC102N43-eYFP (B), NAC102N60-eYFP (C), and NAC102N80-eYFP (D) represented eYFP fused with N-terminal 43, 60 or 80 amino acid residues, respectively. E2Fa-CFP was used as the nucleus marker and the chlorophyll fluorescence was used as the chloroplast marker. The pictures were captured using confocal microscopy. Bars = 10 µm.
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